CN110385107A - A kind of hydrophily adsorbent for bilirubin and its preparation and application - Google Patents
A kind of hydrophily adsorbent for bilirubin and its preparation and application Download PDFInfo
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- CN110385107A CN110385107A CN201810343679.2A CN201810343679A CN110385107A CN 110385107 A CN110385107 A CN 110385107A CN 201810343679 A CN201810343679 A CN 201810343679A CN 110385107 A CN110385107 A CN 110385107A
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- adsorbent
- bilirubin
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/40—Aspects relating to the composition of sorbent or filter aid materials
- B01J2220/48—Sorbents characterised by the starting material used for their preparation
- B01J2220/4812—Sorbents characterised by the starting material used for their preparation the starting material being of organic character
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Abstract
The present invention relates to field of blood purification, and in particular to a kind of hydro-thermal method directly prepares adsorbent for bilirubin and its application.Specific preparation process be take water as a solvent, using glucose as carbon source, quaternary ammonium group is introduced by functionalized reagent of methacryl hydroxypropyltrimonium chloride, only need single step reaction that the fabulous adsorbent of hydrophily can be prepared.Adsorbent of the present invention has good elimination effect to the virulence factor bilirubin of hyperbilirubinemia.
Description
Technical field
The invention belongs to field of blood purification, and in particular to a kind of one step hydro thermal method prepare adsorbent for bilirubin preparation and
Using the adsorbent can be used for the treatment of life and health field hyperbilirubinemia.
Background technique
Hyperbilirubinemia (Hyperbilirubinemia) is since bilirubin generation is excessive or liver cell is red to gallbladder
Obstacle occurs for element intake, combination and discharge process, causes the red concentration of gallbladder in blood to increase, and then cause a system to tissue, organ
Arrange the illness of damage.Jaundice is the exterior representations of hyperbilirubinemia.The appearance of hyperbilirubinemia means that a variety of causes is led
The presence, such as hepatitis, cirrhosis, hepatic failure of serious disease in the liver and gallbladder etc. caused.
Internal excess bilirubin is quickly and effectively removed to alleviation state of an illness right and wrong for serious hyperbilirubinemia patient
It is often necessary.Blood purification therapy can remove plasma in high concentrations bilirubin fast and effeciently not only to alleviate bilirubin toxicity work
With, moreover it is possible to break vicious circle, improves organismic internal environment, temporarily substitute the partial function of liver cell, regenerated for liver cell
And liver function recovery creates conditions or waits chance to carry out liver transfer operation.Blood purification remove bilirubin method have haemodialysis,
Plasma exchange, molecular adsorbent recirculation system (Molecular Adsorption Recycling System, MARS) and blood
Perfusion (Hemoperfusion, HP) etc., wherein blood perfusion due to selective high, few side effects and it is at low cost the advantages that form
For the preferred therapy for treating hyperbilirubinemia.Blood perfusion, which refers to, guides to blood samples of patients in vitro, flows through equipped with adsorbent
Perfusion device is adsorbed using adsorbent and the in vivo interaction between harmful toxicant, and is removed, and blood is reached
The purpose of liquid purification.And safe and efficient adsorbent for bilirubin is the core and key that blood perfusion removes high concentration bilirubin.
The adsorbent for bilirubin clinically applied at present includes acticarbon, synthetic resin adsorbent, immuno absorbence
Agent and affinity adsorbent etc., but there are preparation process mostly complicated, higher cost or it is not environmentally friendly enough the problems such as.
The present invention is taken water as a solvent using hydro-thermal method, and glucose is carbon source, methacryl hydroxypropyltrimonium chloride
(MAPTAC) it is functionalized reagent, the fabulous adsorbent for bilirubin of hydrophily is prepared.
Summary of the invention
The present invention is taken water as a solvent using hydro-thermal method, and glucose is carbon source, and methacryl hydroxypropyltrimonium chloride is
Functionalized reagent introduces quaternary ammonium group, and heating obtains the fabulous adsorbent for bilirubin of hydrophily.
Specifically include following content:
The aqueous solution containing glucose and methacryl hydroxypropyltrimonium chloride is prepared, mixed solution is added and is reacted
Kettle, heating obtain adsorbent for bilirubin.
The mass percent of glucose is 10~20% in above-mentioned reaction, the matter of methacryl hydroxypropyltrimonium chloride
Measuring percentage is 1~20%.
The heating temperature is 160~200 DEG C, and the reaction time is 5~10 hours.
The adsorbent is directly prepared by hydro-thermal method, and absorption aglucon is quaternary ammonium group, and structural representation formula is as follows:
The adsorbent advantage is:
(1) carbon source is made with glucose, it is from a wealth of sources, it is at low cost；
(2) absorption aglucon is the quaternary ammonium group provided by methacryl hydroxypropyltrimonium chloride, and specificity is good；
(3) preparation-obtained adsorbent has good hydrophily.
The preparation process advantage of the adsorbent is:
(1) only need a step that adsorbent can be prepared using hydro-thermal method, it is easy to operate；
(2) nontoxic with water as solvent, environmental protection.
Detailed description of the invention
Fig. 1 adsorbent for bilirubin prepares schematic diagram.
Fig. 2 adsorbent for bilirubin helium ion microscope picture.As can be seen from the figure the microballoon of prepared adsorbent is straight
Diameter is 1~10 micron.
Fig. 3 adsorbent for bilirubin contact angle.As can be seen from the figure prepared adsorbent contact angle is 20~30 °, is said
The bright adsorbent has fabulous hydrophily.
Fig. 4 adsorbent for bilirubin N2Adsorption-desorption isothermal (a) and graph of pore diameter distribution (b).(a) is schemed according to BET equation meter
The specific surface area of calculating is in 30m2/ g is hereinafter, explanation there's almost no pore structure.Exist from can be seen that in adsorbent in figure (b)
Seldom micropore and mesoporous.
Fig. 5 adsorbent for bilirubin adsorption isotherm (a) and dynamic absorption curve (b).From figure (a) it can be seen that its absorption
Capacity increases with the increase of bilirubin equilibrium concentration in a certain range, after equilibrium concentration reaches 150mg/L, adsorption capacity
It is not further added by, about 140mg/g；From figure (b) it can be seen that adsorption process reaches balance in 1h or so, it is to initial concentration
The bilirubin solution adsorption capacity of 200mg/L is about 70mg/g.
Specific embodiment
Embodiment 1
2g glucose is dissolved in 17.6mL water, the methacryl that 0.4g (380 μ L) mass fraction is 50% is added
Hydroxypropyltrimonium chloride (CAS:51410-72-1) solution, mixed solution is added in 50mL reaction kettle, then by reaction kettle
It is put into baking oven, 180 DEG C are heated 6 hours, colourless, the vacuum at 60 DEG C that obtained product is successively washed till supernatant with water and ethyl alcohol
It is spare after 12 hours dry.
The above-mentioned product of 5mg is weighed, is added in the 5mL brown centrifuge tube equipped with 4mL 200mg/L bilirubin solution.Centrifugation
Pipe is placed in 37 DEG C, and concussion speed is that 2h is vibrated under dark condition in the isothermal vibration device of 200r/min, is centrifuged, supernatant is taken to exist
Its absorbance is surveyed under 438nm wavelength, and the mass concentration of bilirubin solution after absorption is calculated by bilirubin standard curve.Bilirubin is inhaled
The calculation formula of attached capacity is as follows:
Q=(C0-C)V/m
Wherein, Q (mg/g) is adsorbance, C0(mg/L) and C (mg/L) is the mass concentration for adsorbing front and back bilirubin solution,
V (L) is bilirubin solution volume, and m (g) is the quality of adsorbent material.
The bilirubin solution adsorption capacity that prepared adsorbent is 200mg/L to concentration is 70mg/g.
Embodiment 2
2g glucose is dissolved in 14mL water, the methacryl propyl that 4g (3.8mL) mass fraction is 50% is added
Trimethyl ammonium chloride (CAS:51410-72-1) solution, mixed solution is added in 50mL reaction kettle, then reaction kettle is put into
In baking oven, 180 DEG C are heated 6 hours, and it is colourless that obtained product is successively washed till supernatant with water and ethyl alcohol, are dried in vacuo at 60 DEG C
It is spare after 12 hours.
The above-mentioned product of 5mg is weighed, is added in the 5mL brown centrifuge tube equipped with 4mL 200mg/L bilirubin solution.Centrifugation
Pipe is placed in 37 DEG C, and concussion speed is that 2h is vibrated under dark condition in the isothermal vibration device of 200r/min, is centrifuged, supernatant is taken to exist
Its absorbance is surveyed under 438nm wavelength, and the mass concentration of bilirubin solution after absorption is calculated by bilirubin standard curve.Bilirubin is inhaled
The calculation formula of attached capacity is as follows:
Q=(C0-C)V/m
Wherein, Q (mg/g) is adsorbance, C0(mg/L) and C (mg/L) is the mass concentration for adsorbing front and back bilirubin solution,
V (L) is bilirubin solution volume, and m (g) is the quality of adsorbent material.
The bilirubin solution adsorption capacity that prepared adsorbent is 200mg/L to concentration is 107mg/g.
Claims (6)
1. a kind of preparation method of hydrophily adsorbent for bilirubin, which is characterized in that using the detailed process of hydro-thermal method are as follows: prepare
Aqueous solution containing glucose and methacryl hydroxypropyltrimonium chloride, mixed solution is added in reaction kettle, heating
Obtain adsorbent for bilirubin.
2. preparation method described in accordance with the claim 1, it is characterised in that: the mass percent of glucose is 10~20%, first
The mass percent of base acryloyl hydroxypropyltrimonium chloride is 1~20%；Hydrothermal temperature is 160~200 DEG C, when reaction
Between be 5~10 hours.
3. a kind of hydrophily adsorbent for bilirubin that preparation method as claimed in claim 1 or 2 prepares.
4. a kind of application of adsorbent described in claim 3, it is characterised in that: it is red that the adsorbent can be applied to gallbladder in solution
The absorption of element.
5. applying according to claim 4, it is characterised in that: the solution be hyperbilirubinemia patient blood and/
Or blood plasma.
6. adsorbent described in a kind of claim 3 is as the adsorbent for bilirubin in hyperbilirubinemia blood samples of patients purification process
Application.
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| CN201810343679.2A CN110385107B (en) | 2018-04-17 | 2018-04-17 | Hydrophilic bilirubin adsorbent and preparation and application thereof |
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| CN201810343679.2A CN110385107B (en) | 2018-04-17 | 2018-04-17 | Hydrophilic bilirubin adsorbent and preparation and application thereof |
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| CN110385107A true CN110385107A (en) | 2019-10-29 |
| CN110385107B CN110385107B (en) | 2021-09-14 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60251110A (en) * | 1984-05-28 | 1985-12-11 | Sumitomo Bakelite Co Ltd | Manufacture of activated carbon |
| JPH06198167A (en) * | 1992-12-28 | 1994-07-19 | Toyobo Co Ltd | Bilirubin adsorbent |
| RU2362733C1 (en) * | 2008-05-19 | 2009-07-27 | Институт проблем переработки углеводородов Сибирского отделения Российской Академии Наук (ИППУ СО РАН) | Method for processing of carbon mesoporous hemosorbent |
| CN101623622A (en) * | 2009-07-28 | 2010-01-13 | 中国科学院上海硅酸盐研究所 | Absorbent material and preparation thereof |
| CN102049242A (en) * | 2011-01-08 | 2011-05-11 | 广州康盛生物科技有限公司 | Anion resin for bilirubin absorption and preparation method thereof |
| CN107486176A (en) * | 2017-09-11 | 2017-12-19 | 广州康盛生物科技有限公司 | A kind of sorbing material for blood purification and preparation method thereof |
-
2018
- 2018-04-17 CN CN201810343679.2A patent/CN110385107B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60251110A (en) * | 1984-05-28 | 1985-12-11 | Sumitomo Bakelite Co Ltd | Manufacture of activated carbon |
| JPH06198167A (en) * | 1992-12-28 | 1994-07-19 | Toyobo Co Ltd | Bilirubin adsorbent |
| RU2362733C1 (en) * | 2008-05-19 | 2009-07-27 | Институт проблем переработки углеводородов Сибирского отделения Российской Академии Наук (ИППУ СО РАН) | Method for processing of carbon mesoporous hemosorbent |
| CN101623622A (en) * | 2009-07-28 | 2010-01-13 | 中国科学院上海硅酸盐研究所 | Absorbent material and preparation thereof |
| CN102049242A (en) * | 2011-01-08 | 2011-05-11 | 广州康盛生物科技有限公司 | Anion resin for bilirubin absorption and preparation method thereof |
| CN107486176A (en) * | 2017-09-11 | 2017-12-19 | 广州康盛生物科技有限公司 | A kind of sorbing material for blood purification and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘章锁等: "《危重急症血液净化治疗学》", 30 September 2017, 河南科学技术出版社 * |
| 谭腾飞等: "氨基功能化碳材料的水热制备及其对活性艳红K-2BP的吸附性能", 《环境工程学报》 * |
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