CN110383976A - A kind of Wheat Seed Vitality test method - Google Patents
A kind of Wheat Seed Vitality test method Download PDFInfo
- Publication number
- CN110383976A CN110383976A CN201810364201.8A CN201810364201A CN110383976A CN 110383976 A CN110383976 A CN 110383976A CN 201810364201 A CN201810364201 A CN 201810364201A CN 110383976 A CN110383976 A CN 110383976A
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- CN
- China
- Prior art keywords
- seed
- wheat seed
- wheat
- ttc
- vigor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of Wheat Seed Vitality test methods, utilize TTC (2, 3, 5- triphenyltetrazolium chloride) wheat seed internal structure is dyed, the vigor of seed is measured according to the color inside wheat seed, operation of the present invention is convenient, it may be implemented quickly to detect wheat seed, TTC is a kind of dissolubility photaesthesia compound, it is pyridine in respiratory chain-nucleotide structure enzyme system proton acceptor, it is combined with the succinate dehydrogenase in seed normal tissue, the vigor of seed is determined in conjunction with rear showed color by observing, detection time is fast, detection effect is good.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of Wheat Seed Vitality test method.
Background technique
With the raising of socio-economic development, people are higher and higher for the quality requirement of food, and food seed vitality
It is the important indicator of seed quality, because can seed vitality can determine seed and neatly emerge rapidly and healthy growth, is planting
Decisive role is played in object growth course.
Summary of the invention
Present invention seek to address that drawbacks described above, provides a kind of Wheat Seed Vitality test method.
In order to overcome defect present in background technique, the technical solution adopted by the present invention to solve the technical problems is: a kind of
Wheat Seed Vitality test method contaminates wheat seed internal structure using TTC (2,3,5- triphenyltetrazolium chloride)
Color measures the vigor of seed according to the color inside wheat seed.
In a preferred embodiment of the present invention, the wheat seed is the dry seed of storage, and dry seed is conducive to
It is completely combined with TTC, it is easier to judge the vigor of seed.
In a preferred embodiment of the present invention, the normal concentration of the TTC solution is 2%, and the dyeing effect of this solubility is most
Obviously, accuracy is higher
The beneficial effects of the present invention are: being dyed using TTC solution and Interior Seed structure, the side of this seed vitality test
Method is faster more efficient, and test effect is obvious, and accuracy is relatively high.
Specific embodiment
A kind of Wheat Seed Vitality test method, includes the next steps:
A. 500g dry seed is randomly selected in seed-box to be tested.
B. the 500g seed of selection is splitted with cutter from centre, seed is placed dyeing box after having cutd open.
C. conventional solubility be 2% TTC solution pour into dyeing box, stand 15-30min, allow seed internal structure sufficiently and
TTC fusion.
D. the seed dyed is taken out observation, if red is presented in Interior Seed structure, it was demonstrated that the vigor of seed is good, if seed
Red is not presented in internal structure, it was demonstrated that seed vitality is poor.
E. the seed that vigor is good and vigor is bad is planned as a whole, the good seed of vigor account for 80% be considered as test pass through.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to be said using the present invention
Equivalent structure or equivalent flow shift made by bright book content is applied directly or indirectly in other relevant technical fields,
Similarly it is included within the scope of the present invention.
Claims (3)
1. a kind of Wheat Seed Vitality test method, it is characterised in that: using TTC (2,3,5- triphenyltetrazolium chloride) to small
Wheat Seeds internal structure is dyed, and the vigor of seed is measured according to the color inside wheat seed.
2. the method for seed vitality test according to claim 1, it is characterised in that: the wheat seed is the drying of storage
Seed.
3. the method for seed vitality test according to claim 1, it is characterised in that: the normal concentration of the TTC solution is
2%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810364201.8A CN110383976A (en) | 2018-04-23 | 2018-04-23 | A kind of Wheat Seed Vitality test method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810364201.8A CN110383976A (en) | 2018-04-23 | 2018-04-23 | A kind of Wheat Seed Vitality test method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110383976A true CN110383976A (en) | 2019-10-29 |
Family
ID=68284290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810364201.8A Pending CN110383976A (en) | 2018-04-23 | 2018-04-23 | A kind of Wheat Seed Vitality test method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110383976A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002066972A2 (en) * | 2001-02-19 | 2002-08-29 | Bayer Bioscience N.V. | Methods and means for determining fitness in plants |
| CN107079632A (en) * | 2016-08-30 | 2017-08-22 | 安徽丰絮农业科技股份有限公司 | A kind of wheat seed quality determining method |
-
2018
- 2018-04-23 CN CN201810364201.8A patent/CN110383976A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002066972A2 (en) * | 2001-02-19 | 2002-08-29 | Bayer Bioscience N.V. | Methods and means for determining fitness in plants |
| CN107079632A (en) * | 2016-08-30 | 2017-08-22 | 安徽丰絮农业科技股份有限公司 | A kind of wheat seed quality determining method |
Non-Patent Citations (1)
| Title |
|---|
| 曹翠玲等编著: "《植物生理学教学实验指导》", 30 September 2016, 西北农林科技大学出版社 * |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191029 |
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| RJ01 | Rejection of invention patent application after publication |