CN110367326A - A kind of meat products biology composite preservative and its preservation method - Google Patents
A kind of meat products biology composite preservative and its preservation method Download PDFInfo
- Publication number
- CN110367326A CN110367326A CN201910781670.4A CN201910781670A CN110367326A CN 110367326 A CN110367326 A CN 110367326A CN 201910781670 A CN201910781670 A CN 201910781670A CN 110367326 A CN110367326 A CN 110367326A
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- Prior art keywords
- meat products
- antistaling agent
- lactic acid
- electric field
- spraying
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- 235000013622 meat product Nutrition 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000002131 composite material Substances 0.000 title claims abstract description 27
- 239000003755 preservative agent Substances 0.000 title claims abstract description 22
- 230000002335 preservative effect Effects 0.000 title claims abstract description 21
- 238000004321 preservation Methods 0.000 title abstract description 7
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 71
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 64
- 238000005507 spraying Methods 0.000 claims abstract description 60
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000004310 lactic acid Substances 0.000 claims abstract description 32
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 32
- 239000007921 spray Substances 0.000 claims abstract description 26
- 229920001661 Chitosan Polymers 0.000 claims abstract description 15
- 108010039918 Polylysine Proteins 0.000 claims abstract description 13
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 13
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 13
- 241001122767 Theaceae Species 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 10
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- 238000007590 electrostatic spraying Methods 0.000 claims abstract description 7
- ODLHGICHYURWBS-LKONHMLTSA-N trappsol cyclo Chemical compound CC(O)COC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)COCC(O)C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1COCC(C)O ODLHGICHYURWBS-LKONHMLTSA-N 0.000 claims abstract description 4
- 230000005684 electric field Effects 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 23
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 17
- 238000005520 cutting process Methods 0.000 claims description 16
- 229910052750 molybdenum Inorganic materials 0.000 claims description 16
- 239000011733 molybdenum Substances 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 7
- 238000009920 food preservation Methods 0.000 claims description 6
- 239000003595 mist Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 abstract description 53
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract description 12
- 238000010008 shearing Methods 0.000 abstract description 7
- 238000011282 treatment Methods 0.000 abstract description 7
- 239000002253 acid Substances 0.000 abstract description 5
- 238000007654 immersion Methods 0.000 abstract description 4
- 238000007610 electrostatic coating method Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 23
- 230000000694 effects Effects 0.000 description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 21
- 239000000523 sample Substances 0.000 description 21
- 239000011344 liquid material Substances 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 15
- 235000013372 meat Nutrition 0.000 description 11
- 238000007605 air drying Methods 0.000 description 10
- 235000013305 food Nutrition 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 229920000858 Cyclodextrin Polymers 0.000 description 9
- 239000008223 sterile water Substances 0.000 description 9
- 238000002525 ultrasonication Methods 0.000 description 9
- 239000011521 glass Substances 0.000 description 8
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 8
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 8
- 229960000935 dehydrated alcohol Drugs 0.000 description 7
- 229960004756 ethanol Drugs 0.000 description 7
- 235000019441 ethanol Nutrition 0.000 description 7
- 238000003958 fumigation Methods 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 230000005686 electrostatic field Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 230000003078 antioxidant effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 229960000583 acetic acid Drugs 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 4
- 239000012496 blank sample Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241001071864 Lethrinus laticaudis Species 0.000 description 3
- 238000000889 atomisation Methods 0.000 description 3
- 239000012362 glacial acetic acid Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
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- 238000009825 accumulation Methods 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- CBNDAMRASFVNKT-UHFFFAOYSA-N 2,5-dihydroxy-2-methylpentanoic acid Chemical compound OC(=O)C(O)(C)CCCO CBNDAMRASFVNKT-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000001116 FEMA 4028 Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 1
- 235000011175 beta-cyclodextrine Nutrition 0.000 description 1
- 229960004853 betadex Drugs 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000002800 charge carrier Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000008204 material by function Substances 0.000 description 1
- 238000005399 mechanical ventilation Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/10—Coating with a protective layer; Compositions or apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/025—Discharge apparatus, e.g. electrostatic spray guns
- B05B5/03—Discharge apparatus, e.g. electrostatic spray guns characterised by the use of gas, e.g. electrostatically assisted pneumatic spraying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of meat products biology composite preservative and its preservation methods, composite preservative includes the chitosan of 1%-3%, the hydroxypropyl-β-cyclodextrin of 1%-2%, the glycerol of 0.1%-1%, the tea polyphenols of 0.1%-0.15%, the epsilon-polylysine of 0.05%-0.1% and the lactic acid aqueous solution of surplus.By the way that the biological composite preservative is equably sprayed at meat products surface using electrostatic spraying processes, formed with a thickness of the film for 0.005-0.018mm.Antistaling agent selection of the present invention lactic acid replaces traditional acetic acid, and using lactic acid to prepare antistaling agent as acid solution not only can guarantee good dissolubility, but also can improve the bacteriostasis property of antistaling agent, while having good environment friendly and safety.Spraying method is assisted using high-pressure electrostatic, compared to tradition spray, immersion process and the electrostatic coating method being atomized using electric current shearing force, there is more preferably controllability, continuity, avoids introducing pollution in treatment process, it is easy to operate.
Description
Technical field
The present invention relates to meat products New Cut Flower Fresh Keeping spraying technology, especially a kind of meat products biology composite preservative and its
Preservation method belongs to food preservative technology field.
Background technique
China is meat products production and consumption big country, and according to data statistics, national meat products yield has just been broken through within 2017
16000000 tons, wherein low-temperature meat product yield accounts for meat products total output 64.3% up to 10,720,000 tons；High temperature meat products yield
Up to 5,950,000 tons, meat products total output 35.7% is accounted for.However meat product water activity is larger, easily cooperating microorganisms, this is to draw
One of an important factor for sending out food-safety problem, lipid material abundant easily oxidative rancidity, complicated enzyme activity will destroy food
Flavor, lead to food quality deterioration.In order to extend the shelf-life of meat product, ensures food safety, corresponding guarantor should be given
Fresh processing guarantees the fresh of meat.
Crude antistaling agent is the functional materials in naturally occurring organism, with antibacterial, antioxidant activity, compared to
Traditional chemical preservative has more safety and environment friendly, passes through answering between the bio-preservative of different proportion
Match, can satisfy the anti-corrosive fresh-keeping needs of different food products.
Traditional antistaling agent processing method often based on impregnating, spray, often results in antistaling agent and is distributed not in food surface
Uniformly, poor adhesive force, material waste are serious.Conventional electrostatic spraying method is atomized drop using electric current shearing force, for
Voltage requires high, and voltage is applied directly in solution and antioxidant composition in liquid material is be easy to cause to be denaturalized, liquid material spray efficiency compared with
It is low.
Summary of the invention
The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, provide a kind of natural composite antistaling agent,
Addition natural antiseptic agent assigns its good antibacterial, anti-oxidation function, has good fresh-keeping effect and environment friendly.This hair
It is bright to further provide for a set of antistaling agent electrostatic auxiliary spray equipment, it solves traditional antistaling agent and is unevenly distributed in food surface, it is attached
Put forth effort difference, the serious problem of material waste makes antistaling agent apply more uniform, and adhesive force more preferably, is forming condition of equivalent thickness film
Under conditions of layer, required liquid material is less.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is as follows:
A kind of meat products biology composite preservative, the component including following mass percent:
Surplus is lactic acid aqueous solution.
Wherein, chitosan is as thickener, fruit glaze agent, and for the preparation of food edible film, but its water-soluble is poor,
The acid solution for being dissolved in certain dilution is generally selected, experiment discovery not only can make solution reach ideal using lactic acid
Solubility, the antibacterial attribute of lactic acid itself can also improve the fresh keeping property of solution.
Tea polyphenols are as plant anti-oxidation component, mainly as antioxidant.
Hydroxypropyl-β-cyclodextrin has the active constituent in solution, antioxidant composition as stabilizer/eliminating smell agent
Certain protective role, and can effectively remove the bad smell in solution.
The adhesion of antistaling agent and surface of material, optimization film forming effect can be improved as thickener/surfactant in glycerol
Fruit.
Epsilon-polylysine is as the antibacterial component of biology, mainly as bacteriostatic preservative.
Preferably, the mass concentration of the lactic acid aqueous solution is 2~5%.
Claimed meat products biology composite preservative conductivity is 1420-1670 μ s/cm.
Above-mentioned meat products biology composite preservative is prepared via a method which: chitosan being added in lactic acid aqueous solution, together
When hydroxypropyl-β-cyclodextrin is added, stirred 2~3 hours at 50~60 DEG C, add glycerol, tea polyphenols, epsilon-polylysine,
Filtering, ultrasonic wave exclude bubble after mixing evenly at room temperature to obtain the final product.
Further, the present invention provides the method that above-mentioned meat products biology composite preservative carries out food preservation, by institute
The biological composite preservative stated equably is sprayed at meat products surface using electrostatic spraying processes, is formed with a thickness of 0.005-
The film of 0.018mm.
Specifically, the electrostatic spraying processes carry out the sprinkling of antistaling agent, the electrostatic spray dress using electrostatic atomizer
It sets including spraying box, master control cabinet, fluid reservoir and compressed air hose；
The spraying box bottom is equipped with idler wheel, and idler wheel is equipped with the charging tray for placing meat products to be sprayed；Spraying box top
Portion is equipped with electric field case, the electric field case bottom opening；
The feed liquid pipe of the fluid reservoir enters the electric field case in spraying box after master control cabinet, together with compressed air hose
It is connected to the feed liquid spout of electric field box top；
The master control cabinet connects the wire cutting molybdenum filament in electric field bottom portion by high voltage transmission line.
Antistaling agent liquid material is mixed in feed liquid nozzle with compressed air, and uniform mist is dispersed under the action of air stream shear forces
Drop, wafts and brims in air-flow encapsulation under gravity vertically downwards；Electric field case interior lines cutting molybdenum wire is below spout
Annular section is surrounded, principle of corona discharge is utilized after energization, strong electrical field is generated in annular section, electric field domain shape, size can
It is obtained according to actual needs by adjusting the annular section that wire electrode surrounds.
The droplet wafted and brimed with downwards charges rapidly in high voltage electric field, and the meat products to be sprayed in charging tray compares droplet and is in
Heteropolar current potential, since electrostatic adsorption and meat products are combined closely when the droplet under charge carrier state is infected with meat products；Together
When homocharge droplet between there are electrostatic repulsions, the accumulation phenomena of meat products surface the same area is effectively reduced, after spraying
Natural air drying or mechanical ventilation drying.
The flow controller for controlling feed velocity in feed liquid pipe is equipped in the master control cabinet, and for cutting online
The HV generator that high-voltage electrostatic field is formed around molybdenum filament is cut, HV generator can provide negative high voltage, make electric field
Charge on wire cutting molybdenum filament band in case.
Further, the wire cutting molybdenum filament is fixed on electric field bottom portion by insulating support；The insulating support is two
A, left and right places and is parallel to each other, and each bracket is equipped with one group of through-hole, and wire cutting molybdenum filament passes through through-hole and is wrapped in two insulation
Between bracket；The wire cutting molybdenum filament one terminates negative high voltage electrostatic, and the other end is wrapped in through hole, is not formed into a loop.
Preferably, feed velocity is 5-10L/h in the flow controller control feed liquid pipe；The HV generator
Generating voltage is 30-65kV；Gas pressure is 2-4kg/cm in the compressed air hose2；So that spraying mist droplet particle size control exists
250-448nm；The feed liquid spout single spin time is 10-30s.
The utility model has the advantages that
1, currently, the preparation of chitosan-based antistaling agent is often using acetic acid as acid solution, the present invention selects to be taken with lactic acid
Generation.Lactic acid inherently has certain bacteriostasis property, is usually used in the fresh-keeping of meat and meat products, while can also provide certain acid
Property, using lactic acid as acid solution preparation antistaling agent not only can guarantee good dissolubility, but also can improve the bacteriostasis property of antistaling agent,
There is good environment friendly and safety simultaneously.Antistaling agent, green non-pollution, safety are prepared using natural function ingredient
Property is reliable.
2, the present invention assists spraying method using high-pressure electrostatic, compared to tradition spray, immersion process, has and more preferably may be used
Control property, continuity avoid introducing pollution in treatment process, easy to operate.It is quiet compared to being atomized using electric current shearing force
EFI coating method, the present invention are atomized using the air stream shear forces of gas-pressurized, voltage are not applied directly in liquid material,
Safer, equipment is simple, and cost is reasonable, is conducive to further industrialization promotion.Using gas-pressurized to antistaling agent liquid material
It is atomized, atomizing effect is then optimized by electrostatic field, it is more efficient compared to the method using the atomization of electric current shearing force, together
When can preferably protect antioxidant content, antibacterial substance in antistaling agent.
Detailed description of the invention
The present invention is done with reference to the accompanying drawings and detailed description and is further illustrated, of the invention is above-mentioned
And/or otherwise advantage will become apparent.
Fig. 1 is the structural schematic diagram that electrostatic of the present invention assists spray equipment.
Fig. 2 is the total plate count curve that small griskin sample is sprayed under different current field conditions.
Fig. 3 is the total plate count curve comparison figure of comparative example 1 and embodiment 1.
Fig. 4 is the total plate count curve comparison figure of comparative example 2 and embodiment 1.
Fig. 5 is the total plate count curve comparison figure of comparative example 3 and embodiment 1.
Fig. 6 is the total plate count curve comparison figure of comparative example 4 and embodiment 1.
Fig. 7 is variation of the different spraying methods with voltage change antistaling agent to Escherichia coli (E.Coli) bacteriostasis rate.
Fig. 8 is that 4 DEG C of sample states after preservation 5 days are sprayed after small griskin sample under different current field conditions.
Specific embodiment
According to following embodiments, the present invention may be better understood.
Structure depicted in Figure of description, ratio, size etc., only to cooperate the revealed content of specification, with
Understand for those skilled in the art and read, be not intended to limit the invention enforceable qualifications, therefore does not have technically
Essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size can be generated not influencing the present invention
Under effect and the purpose that can reach, should all still it fall in the range of disclosed technology contents can cover.Meanwhile
Cited such as "upper", "lower", "front", "rear", " centre " term in this specification are merely convenient to being illustrated for narration, and
It is non-that to limit the scope of the invention, relativeness is altered or modified, under the content of no substantial changes in technology, when
Also it is considered as the enforceable scope of the present invention.
Following embodiment is all made of the spraying that device as shown in Figure 1 carries out meat products biology composite preservative.The device packet
Include spraying box 1, master control cabinet 2, fluid reservoir 3 and compressed air hose 4.
1 bottom of spraying box is equipped with idler wheel 11, and idler wheel is equipped with the charging tray 12 for placing meat products to be sprayed；Spraying box 1
Top is equipped with electric field case 13,13 bottom opening of electric field case.The feed liquid pipe 31 of fluid reservoir 3 enters spray after master control cabinet 2
The electric field case 13 in case 1 is applied, is connected to the feed liquid spout 15 at 13 top of electric field case together with compressed air hose 4.Master control cabinet 2 is logical
Cross the wire cutting molybdenum filament 14 that high voltage transmission line 21 connects 13 bottom of electric field case.Wire cutting molybdenum filament 14 is fixed on electricity by insulating support
Field 13 bottom of case；The insulating support is two, and left and right places and is parallel to each other, and each bracket is equipped with one group of through-hole, and line is cut
Molybdenum filament 14 is cut to be wrapped in two insulating supports across through-hole；The wire cutting molybdenum filament 14 1 terminates negative high voltage electrostatic, another
End is wrapped in through hole, is not formed into a loop.
The flow controller for controlling feed velocity in feed liquid pipe 31 is equipped in master control cabinet 2, and for cutting online
Cut the HV generator that high-voltage electrostatic field is formed around molybdenum filament.
To guarantee that spray treatment reaches good film-formation result and fresh-keeping effect, We conducted a series of preliminary experiments.
2% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；1mL glycerol is added；It is stirred 2 hours at 50 DEG C；Ultrasonication bubble removing after filtering is made multiple
Close antistaling agent solution.
Liquid material flow velocity is adjusted, discovery atomizing effect when flow velocity is greater than 10L/h is bad, and liquid material is easily dripped at stock, and flow velocity is worked as
When less than 5L/h, liquid material spray efficiency is low, and longer spray time is needed just to can guarantee film-formation result, so selection 5-10L/h
Liquid material flow velocity is practical section；Corresponding, gas pressure is controlled in 2-4kg/cm2With preferable spraying effect.
Economically consider, selects feed velocity 5L/h, gas pressure 2kg/cm2Under the conditions of spray smooth surface glass plate, when
When without electric field-assisted, spraying 10s can guarantee liquid material uniform fold glass plate surface, and when spray time is less than 10s, liquid material is in irregular
Plaque-like is distributed in glass plate surface, cannot cover glass plate, while accumulation effect of the liquid material on treating material surface is limited, works as spraying
Extra liquid material falls off from surface of material when time is greater than 30s, causes to waste, so select 10~30s for practical spray time；It adjusts
Voltage is saved, unobvious for spraying effect improvement when observing that voltage is less than 30kV, 2 total plate count curve of attached drawing also illustrates that,
Auxiliary spraying antistaling agent can be effectively improved fresh-keeping effect under 40kV and the above voltage, under 65kV voltage for spraying effect, protect
Fresh effect has had clear improvement, so select 30-65kV for practical voltage range.
Select feed velocity 5L/h, gas pressure 2kg/cm2Under the conditions of spray smooth surface glass plate, select voltage 40kV, 50kV,
60kV, spraying 10s is parallel with two groups of 20s under each voltage conditions, by glass plate natural air drying after spray treatment, with laser film thickness gauge
Film forming thickness in glass plate is measured, every piece of glass plate takes three measurement points, the final film forming thickness for determining antistaling agent in smooth surface glass plate
For 0.005~0.018mm.
It is final to determine spraying parameter are as follows: it is 5-10L/h that flow controller, which controls feed velocity in feed liquid pipe 31,；It is described
HV generator generating voltage is 30-65kV；Gas pressure is 2-4kg/cm in the compressed air hose 42.Feed liquid spout
15 single spin times were 10-30s, were 0.005-0.018mm in the film thickness of meat products surface to be sprayed spraying antistaling agent.
Embodiment 1
2% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；It is stirred 2 hours at 50 DEG C；1mL glycerol is added, 0.2g epsilon-polylysine, 0.2g tea polyphenols are in molten
In liquid；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Use dehydrated alcohol pair
Fumigation is carried out in spraying box；After ethyl alcohol volatilizees completely, commercially available fresh small griskin about 100g is taken to be placed in clean charging tray
On；Antistaling agent feed velocity is adjusted to 5L/h, gas pressure 2kg/cm2, voltage 60kV；Material inlet valve is opened, is in antistaling agent
Mist is sprayed is pushed into spraying area for charging tray from left to right, timing contacting antistaling agent since fresh small griskin, spraying
20s；Valve is closed, material is taken out, is refrigerated at 4 DEG C after natural air drying.
Embodiment 2
3% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 4g and lactic acid solution is added；Weigh 4g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；It is stirred 2 hours at 50 DEG C；Be added 1mL glycerol, 0.15g epsilon-polylysine, 0.15g tea polyphenols in
In solution；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Use dehydrated alcohol
To carrying out fumigation in spraying box；After ethyl alcohol volatilizees completely, commercially available fresh grade breast about 70g is taken to be placed on clean charging tray；
Antistaling agent feed velocity is adjusted to 7L/h, gas pressure 2.5kg/cm2, voltage 40kV；Material inlet valve is opened, is in mist to antistaling agent
Shape is sprayed is pushed into spraying area for charging tray from left to right, and timing contacting antistaling agent since fresh grade breast sprays 15s；It closes
Valve takes out material, is refrigerated after natural air drying at 4 DEG C.
Embodiment 3
5% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 4g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；It is stirred 2 hours at 50 DEG C；2mL glycerol is added, 0.2g epsilon-polylysine, 0.2g tea polyphenols are in molten
In liquid；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Use dehydrated alcohol pair
Fumigation is carried out in spraying box；After ethyl alcohol volatilizees completely, commercially available fresh calf tenterloin about 90g is taken to be placed on clean charging tray；
Antistaling agent feed velocity is adjusted to 8L/h, gas pressure 3kg/cm2, voltage 50kV；Material inlet valve is opened, it is fog-like to antistaling agent
It sprays and charging tray is pushed into spraying area from left to right, timing contacting antistaling agent since fresh calf tenterloin sprays 25s；It closes
Valve takes out material, is refrigerated after natural air drying at 4 DEG C.
Comparative example 1
2% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；It is stirred 2 hours at 50 DEG C；1mL glycerol is added, 0.2g epsilon-polylysine, 0.2g tea polyphenols are in molten
In liquid；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Use dehydrated alcohol pair
Fumigation is carried out in spraying box；After ethyl alcohol volatilizees completely, commercially available fresh small griskin about 100g is taken to be placed in clean charging tray
On；Antistaling agent feed velocity is adjusted to 5L/h, gas pressure 2kg/cm2；Material inlet valve is opened under the conditions of no electric field-assisted, to
Charging tray is pushed into spraying area by antistaling agent ejection fog-like from left to right, is counted contacting antistaling agent since fresh small griskin
When, spray 20s；Valve is closed, material is taken out, is refrigerated at 4 DEG C after natural air drying.
The comparative example and Examples 1 to 3 products obtained therefrom total plate count curve are shown in Fig. 2, and the comparative example and blank sample are implemented
1 products obtained therefrom total plate count curve of example is shown in Fig. 3, it is seen that electric field-assisted atomizing spraying can significantly improve fresh-keeping effect.
Comparative example 2
2% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；It is stirred 2 hours at 50 DEG C；1mL glycerol is added, 0.2g epsilon-polylysine, 0.2g tea polyphenols are in molten
In liquid；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Take commercially available fresh piggy
Pig loin about 100g impregnates 20s；Material is taken out, is refrigerated at 4 DEG C after natural air drying.
When immersion treatment sample, antistaling agent dosage, which is subject to, to be guaranteed that sample to be processed is totally submerged and needs about 80mL antistaling agent
Liquid material, it is i.e. discarded after immersion treatment, the meat of different batches can not be handled, otherwise will lead to cross contamination.Electrostatic under on year-on-year basis
Auxiliary spraying method handles 20s under 5L/h spraying rate and about consumes 28mL sample.
The comparative example and blank sample, 1 products obtained therefrom total plate count curve of embodiment are shown in Fig. 4, it is seen that the present invention not only can
The usage amount of antistaling agent is enough effectively reduced, while fresh-keeping effect can be significantly improved.
Comparative example 3
2% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；1mL glycerol is added, is stirred 2 hours at 50 DEG C；Ultrasonication bubble removing after filtering is made multiple
Close antistaling agent solution；Using dehydrated alcohol to carrying out fumigation in spraying box；After ethyl alcohol volatilizees completely, take commercially available fresh small
Griskin about 100g is placed on clean charging tray；Antistaling agent feed velocity is adjusted to 5L/h, gas pressure 2kg/cm2, voltage
60kV；Material inlet valve is opened, charging tray is pushed into spraying area from left to right to antistaling agent ejection fog-like, from fresh piggy tenterloin
Meat contact antistaling agent starts timing, sprays 20s；Valve is closed, material is taken out, is refrigerated at 4 DEG C after natural air drying.
Compared with Example 1, which is not added with epsilon-polylysine and tea polyphenols, the comparative example and blank sample, reality
It applies 1 products obtained therefrom total plate count curve of example and sees Fig. 5, it is seen that the addition of epsilon-polylysine and tea polyphenols can effectively improve fresh-keeping
Effect.
Comparative example 4
2% glacial acetic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxypropyl-
Lactic acid solution is added in beta-cyclodextrin；It is stirred 2 hours at 50 DEG C；Be added 1mL glycerol, 0.2g epsilon-polylysine, 0.2g tea polyphenols in
In solution；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Use dehydrated alcohol
To carrying out fumigation in spraying box；After ethyl alcohol volatilizees completely, commercially available fresh small griskin about 100g is taken to be placed in clean material
On disk；Antistaling agent feed velocity is adjusted to 5L/h, gas pressure 2kg/cm2, voltage 60kV；Material inlet valve is opened, to antistaling agent
Charging tray is pushed into spraying area by ejection fog-like from left to right, timing contacting antistaling agent since fresh small griskin, spraying
20s；Valve is closed, material is taken out, is refrigerated at 4 DEG C after natural air drying.
Compared with Example 1, which prepares antistaling agent, the comparative example as base fluid using traditional glacial acetic acid solution
Fig. 6 is seen with blank sample, 1 products obtained therefrom total plate count curve of embodiment, it is seen that lactic acid solution substitutes glacial acetic acid solution as base
The antistaling agent that liquid is prepared has better fresh-keeping bacteriostatic performance.
Comparative example 5
50mL specification injector for medical purpose is taken, piston portion is sealed, replaces piston to push liquid material with peristaltic pump, and by high pressure
Line is installed at metal needle, so that simple high-voltage electrostatic spraying apparatus, i.e. electric current shearing force atomizing spraying be made.
LB solid medium 200mL is prepared, pour plate is several, stands solidification；Take Escherichia coli (E.Coli) enrichment training
Bacteria liquid carries out ten times of dilutions, until 10-4Dilution draws 100 μ L 10-4Dilution bacterium solution to plate is coated, be made to
Handle sample.
2% lactic acid solution 200mL is prepared with sterile water；It weighs chitosan 3g and lactic acid solution is added；Weigh 2g hydroxy propyl-Beta-
Lactic acid solution is added in cyclodextrin；It is stirred 2 hours at 50 DEG C；1mL glycerol is added, 0.2g epsilon-polylysine, 0.2g tea polyphenols are in molten
In liquid；It stirs 1 hour at room temperature；Composite fresh-keeping agent solution is made in ultrasonication bubble removing after filtering；Use dehydrated alcohol pair
Fumigation is carried out in spraying box；After ethyl alcohol volatilizees completely, sample to be processed is taken to be placed on clean charging tray；Adjust antistaling agent material
Flow velocity is to 5L/h, voltage 40kV, 50kV, 60kV；Stablize to antistaling agent and spray, charging tray is pushed into spraying area from left to right,
Timing contacting antistaling agent since fresh small griskin, sprays 8s；Power supply is closed, material is taken out, it is cold at 4 DEG C after natural air drying
Hiding.
Compared with Example 1, which is atomized the spraying method of antistaling agent using electric current shearing force, to ensure antistaling agent
It sprays that dosage is moderate, and spray time is reduced to 8s, and judges two kinds of spraying methods to the shadow of antistaling agent stability with bacteriostasis rate
It rings, the change curve of two kinds of antistaling agent spraying method sterilization rates is shown in Fig. 7.
Table 1 gives variation of the different spraying methods with voltage change antistaling agent to Escherichia coli (E.Coli) bacteriostasis rate.
Table 1
| Voltage (kV) | Gas-pressurized+electrostatic field atomization | The atomization of electric current shearing force |
| 0 | 91.35% | 91.12% |
| 30 | 93.43% | 93.06% |
| 40 | 97.08% | 96.43% |
| 50 | 95.72% | 94.18% |
| 60 | 94.68% | 90.51% |
Note: the calculation method of bacteriostasis rate is referring to formula: bacteriostasis rate=1-n1/n0× 100%, n in formula0It is flat for blank control
Plate clump count, n1For flat-plate bacterial colony number after addition chitosan；Wherein n0=5099.
By being tested above it is found that antistaling agent bacteriostasis rate can be improved under lower voltage effect in two kinds of spraying methods, but works as
After voltage increases to a certain size, sterilization rate continue to increase with voltage it is on a declining curve, this is because strong voltage effect under protect
Fresh dose of functional component is destroyed, fresh-keeping effect decline, and electrostatic auxiliary spraying method of the invention does not directly apply voltage
It is smaller to antistaling agent destruction in antistaling agent liquid material, therefore in contrast, especially under high voltage effect, for the guarantor of antistaling agent
Shield effect becomes apparent.
Table 2 gives the small griskin sample total plate count variation of Different treatments.
Table 2
Note: the safe national standard of measuring method reference food " the GB4789.2-2016 Food Microbiology inspection of total plate count
Test total plate count measurement ", unit CFU/g.
By being tested above it is found that can effectively extend piggy tenterloin using the method that high-voltage electrostatic field assists spraying antistaling agent
The shelf life of meat, according to national regulations, meat total plate count is more than the rotten meat of 6CFU/g, and the meat without any processing is big
General 5th day rotten, and comparative example 1,2 is probably rotten at the 6th day, and the method using high-voltage electrostatic field auxiliary spraying can then make meat
To 10 days, and after observing electric field-assisted spraying, there is downward trend to shelf life extension in total plate count, it was demonstrated that in spraying process
There are certain bactericidal effects to food material for electric field.
Fig. 8 gives small griskin sample is sprayed under different current field conditions after 4 DEG C of sample states after preservation 5 days, wherein a sample
Product are the spraying sample under no current field condition, and b sample is auxiliary spraying sample under 40kV voltage, and c sample is auxiliary under 50kV voltage
Spraying sample is helped, d sample is auxiliary spraying sample under 60kV voltage, is observed within stored refrigerated 5 days for 4 DEG C after processing, a, b sample table
There is canescence, surface tack in face, and meat fiber obviously follows the string, and has unusual odor, and c, d sample sensory evaluation are good,
In addition to slight peculiar smell, do not observe that meat is rotten.
The present invention provides the thinking and method of a kind of meat products biology composite preservative and its preservation method, specific implementations
There are many method and approach of the technical solution, the above is only a preferred embodiment of the present invention, it is noted that for this skill
For the those of ordinary skill in art field, various improvements and modifications may be made without departing from the principle of the present invention,
These modifications and embellishments should also be considered as the scope of protection of the present invention.Each component part being not known in the present embodiment can be used existing
Technology is realized.
Claims (9)
1. a kind of meat products biology composite preservative, which is characterized in that the component including following mass percent:
Surplus is lactic acid aqueous solution.
2. meat products biology composite preservative according to claim 1, which is characterized in that the quality of the lactic acid aqueous solution
Concentration is 2%-5%.
3. meat products biology composite preservative according to claim 1, which is characterized in that its conductivity is 1420-
1670μs/cm。
4. the preparation method of meat products biology composite preservative described in claim 1, which is characterized in that lactic acid is added in chitosan
In aqueous solution, while hydroxypropyl-β-cyclodextrin is added, is stirred 2~3 hours at 50~60 DEG C, add glycerol, tea polyphenols,
Epsilon-polylysine, filtering, ultrasonic wave exclude bubble after mixing evenly at room temperature to obtain the final product.
5. the method for carrying out food preservation using meat products biology composite preservative described in claim 1, which is characterized in that will
The biological composite preservative is equably sprayed at meat products surface using electrostatic spraying processes, is formed with a thickness of 0.005-
The film of 0.018mm.
6. the method for food preservation according to claim 5, which is characterized in that the electrostatic spraying processes use electrostatic
Mist device carries out the sprinkling of antistaling agent, and electrostatic atomizer includes spraying box (1), master control cabinet (2), fluid reservoir (3) and pressure
Contracting air hose (4)；
Spraying box (1) bottom is equipped with idler wheel (11), and idler wheel is equipped with the charging tray (12) for placing meat products to be sprayed；Spray
It applies and is equipped with electric field case (13) at the top of case (1), electric field case (13) bottom opening；
The feed liquid pipe (31) of the fluid reservoir (3) enters the electric field case (13) in spraying box (1) after master control cabinet (2), with
Compressed air hose (4) is connected to the feed liquid spout (15) at the top of electric field case (13) together；
The master control cabinet (2) connects the wire cutting molybdenum filament (14) of electric field case (13) bottom by high voltage transmission line (21).
7. the method for food preservation according to claim 6, which is characterized in that be equipped with and use in the master control cabinet (2)
It is quiet in the flow controller of control feed liquid pipe (31) interior feed velocity, and for forming high pressure around cutting on line molybdenum filament (14)
The HV generator of electric field.
8. the method for food preservation according to claim 6, which is characterized in that the wire cutting molybdenum filament (14) passes through exhausted
Edge bracket is fixed on electric field case (13) bottom；The insulating support is two, and left and right places and is parallel to each other, and is set on each bracket
There is one group of through-hole, wire cutting molybdenum filament (14) is wrapped in two insulating supports across through-hole；The wire cutting molybdenum filament (14) one
Negative high voltage electrostatic is terminated, the other end is wrapped in through hole, is not formed into a loop.
9. the method for food preservation according to claim 8, which is characterized in that the flow controller controls feed liquid pipe
(31) interior feed velocity is 5-10L/h；The HV generator generating voltage is 30-65kV；The compressed air hose (4)
Interior gas pressure is 2-4kg/cm2；Feed liquid spout (15) the single spin time is 10-30s.
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Application publication date: 20191025 |