CN110361680A - A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method - Google Patents
A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method Download PDFInfo
- Publication number
- CN110361680A CN110361680A CN201910542558.5A CN201910542558A CN110361680A CN 110361680 A CN110361680 A CN 110361680A CN 201910542558 A CN201910542558 A CN 201910542558A CN 110361680 A CN110361680 A CN 110361680A
- Authority
- CN
- China
- Prior art keywords
- data
- dimension
- pulse
- sampling module
- super
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
- G01R33/38—Systems for generation, homogenisation or stabilisation of the main or gradient magnetic field
- G01R33/387—Compensation of inhomogeneities
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
- G01R33/48—NMR imaging systems
- G01R33/54—Signal processing systems, e.g. using pulse sequences ; Generation or control of pulse sequences; Operator console
- G01R33/56—Image enhancement or correction, e.g. subtraction or averaging techniques, e.g. improvement of signal-to-noise ratio and resolution
- G01R33/565—Correction of image distortions, e.g. due to magnetic field inhomogeneities
- G01R33/56572—Correction of image distortions, e.g. due to magnetic field inhomogeneities caused by a distortion of a gradient magnetic field, e.g. non-linearity of a gradient magnetic field
Abstract
The super-resolution nuclear magnetic resonance phase sensitivity two dimension J that the invention proposes a kind of for obtaining molecular structure information and accurate measurement J coupling constant decomposes spectral method.This method is uncoupled the collective effect of module and echo train sampling module based on ZS, overcomes deficiency of the existing two dimension J spectral technology in practical application, high-resolution two dimension phase sensitivity J can be obtained under non-uniform magnetic field environment and decomposes spectrum.This method improves the spectra resolution rate that two-dimentional J decomposes spectrum, is of great significance for the structure detection of the complex sample with crowded spectral peak, extends the application field that two-dimentional J decomposes spectrum.Meanwhile on the basis of monochromatic pulses exciting method, invention further provides the sensitivity Enhancement Methods realized based on the sampling of polychrome pulse excitation.
Description
Technical field
The present invention relates to the detections of nuclear magnetic resonance (Nuclear Magnetic Resonance, NMR) Wave Spectrum molecular structure
Technology decomposes the universal method of spectrum more particularly, to a kind of acquisition super-resolution two dimension phase sensitivity J, and in non-uniform magnetic field environment
The lower accurate method for obtaining molecular structure information.
Background technique
As a kind of steady Noninvasive detection technique, NMR spectrum is learned in chemistry, biology and life science etc.
Section field has a wide range of applications.Especially two dimension J decomposes spectrum, as one of most important nuclear magnetic resonance technique, by two
Chemical shift and J coupling information are shown in a independent dimension respectively, provides more molecular structure informations, thus it is extensive
Ground is applied to the measurement of the ownership and J coupling constant of chemical constituent.Existing acquisition two dimension J decomposes most common method in spectrum
It is a kind of based on be continuously increased develop the time spin echo module realize.The sequence is by Nobel chemistry Prize winner earliest
What Switzerland's scientist's Ernest & Whitney was proposed in 1976.However, in practical applications, which is primarily present both sides limit
System.First, due to conventional two-dimensional J decompose spectrum experiment in phase-modulation effect, the technology obtain two-dimentional J decompose spectrum usually with
Absolute value mode is shown, reduces spectra resolution rate.This resolution problem further results in conventional two-dimensional J and decomposes spectral method hardly possible
To be applied to the crowded complex sample detection of spectral peak.Especially, for the sample detection comprising being coupled system by force, conventional two-dimensional J
It decomposes spectrum and often shows strong coupling artifact, to bring the difficulty of signal assignment.Second, conventional two-dimensional J decompose obtaining for spectrum
It takes and higher requirement often is proposed to uniformity of magnetic field, it usually needs can just obtain satisfied two dimension under uniform magnetic field environment
J decomposes spectrum.And for the detection of sample heterogeneous for toughness sample and biological tissue samples etc., the intrinsic magnetization of sample
Unevenly caused Magnetic field inhomogeneity usually masks all useful structural informations, and the magnetic susceptibility that this sample is intrinsic to rate
It is unevenly generally difficult to operate by shimming and eliminate.Therefore, the influence for reducing Magnetic field inhomogeneity degree and strong coupling artifact, obtains super
High-resolution phase sensitivity two dimension J decomposes spectrum, for analysis of the molecular structure and and the accurate measurement of J coupling there is important application meaning.
Summary of the invention
In view of the above technical problems, it is a primary object of the present invention to propose it is a kind of for obtain molecular structure information and
The super-resolution nuclear magnetic resonance phase sensitivity two dimension J of accurate measurement J coupling constant decomposes spectrum.This method is based on ZS and uncouples module and echo
The collective effect of chain sampling module overcomes conventional two-dimensional J and decomposes deficiency of the spectral technology in practical application, effectively eliminates
The influence of Magnetic field inhomogeneity, can obtain high-resolution under non-uniform magnetic field environment on space slice selective gradient direction (direction z)
Two-dimentional phase sensitivity J decomposes spectrum, can be used for structure elucidation and the component analysis of sample, is point of heterogeneous sample and biological tissue samples
Analysis identification provides a kind of detection means.This method improves the spectra resolution rate that two-dimentional J decomposes spectrum, for crowded with spectral peak
Complex sample structure detection and signal assignment be of great significance, greatly expand the application field that two-dimentional J decomposes spectrum.
In order to solve the above technical problems, the present invention provides a kind of super-resolution nuclear magnetic resonance two dimension J to decompose spectrum side
Method includes the following steps:
1) sample is sent into the test chamber of nuclear magnetic resonance spectrometer；
2) emit radio-frequency pulse and gradient pulse sequence, realize the evolution and separation of sample chemical displacement and J coupling, thus
Show chemical shift and J coupling information respectively in chemical shift peacekeeping J coupling dimension；
The pulse train is by pi/2 non-selective excitation pulse, and uncouple module and echo train sampling module of ZS is constituted；
The ZS uncouple module include first dephasing gradient pulse, a non-selective pulse of π, second dephasing ladder
Spend pulse, a π selective pulse and the space slice selective gradient applied simultaneously with selective pulse, third dephasing gradient arteries and veins
Punching；Wherein the direction of all gradient pulses is identical, and along Z-direction；
The echo train sampling module includes the sampling module for repeating 2N times, and each sampling module is by one section of sampling time
The non-selective pulse composition of T and π；
3) data sampling and Data Post are carried out, counting to obtain has resistance Magnetic field inhomogeneity and the strong coupling artifact of reduction excellent
The super-resolution nuclear magnetic resonance phase sensitivity two dimension J of gesture decomposes spectrum.
In a preferred embodiment: the process of the step 4) data processing is as follows:
(a) since the odd-times sampling module data collected in only echo train sampling module are the useful number needed
According to, odd and even data is separated during processing, individually extract odd-times data handled；
It (b) is a two-dimensional data matrix by odd-times data reconstruction；
(c) two-dimensional Fourier transform is carried out to obtained 2-D data, and carries out phase modulation operation, can be obtained a Zhang Chaogao
The phase sensitivity two dimension J of resolution decomposes spectrum.
In a preferred embodiment: specific steps are as follows: the specific practice of the step b of data processing are as follows:
By the data in first sampling module of the sampled data of each indirect dimension evolution incremental time in sampling time T
Point carries out sequential concatenation, arranges to reconstruct the first row of two-dimensional matrix, successively will be in the sampling time in (2N+1) a sampling module
Data point row for reconstruct two-dimensional matrix Nth row, to obtain required two-dimensional data matrix.
The present invention also provides a kind of super-resolution nuclear magnetic resonance two dimension J to decompose spectral method, includes the following steps:
1) sample is sent into the test chamber of nuclear magnetic resonance spectrometer；
2) emit radio-frequency pulse and gradient pulse sequence, realize the evolution and separation of sample chemical displacement and J coupling, thus
Show chemical shift and J coupling information respectively in chemical shift peacekeeping J coupling dimension；
The pulse train is by pi/2 non-selective excitation pulse, and uncouple module and echo train sampling module of ZS is constituted；
The ZS uncouple module include first dephasing gradient pulse, a non-selective pulse of π, second dephasing ladder
Spend pulse, a π selective pulse and the space slice selective gradient applied simultaneously with selective pulse, third dephasing gradient arteries and veins
Punching；Wherein the direction of all gradient pulses is identical, and along Z-direction；A π selective pulse is the multiple Frequency points of excitation
Polychrome pulse；
The echo train sampling module includes the sampling module for repeating 2N times, and each sampling module is by one section of sampling time
The non-selective pulse composition of T and π；
3) data sampling and Data Post are carried out, counting to obtain has resistance Magnetic field inhomogeneity and the strong coupling artifact of reduction excellent
The super-resolution nuclear magnetic resonance phase sensitivity two dimension J of gesture decomposes spectrum.
In a preferred embodiment: the process of the step 4) data processing is as follows:
(a) since the odd-times sampling module data collected in only echo train sampling module are the useful number needed
According to, odd and even data is separated during processing, individually extract odd-times data handled；
It (b) is a two-dimensional data matrix by odd-times data reconstruction；
(c) two-dimensional Fourier transform is carried out to obtained 2-D data, and carries out phase modulation operation, can be obtained a Zhang Chaogao
The phase sensitivity two dimension J of resolution decomposes spectrum.
In a preferred embodiment: specific steps are as follows: the specific practice of the step b of data processing are as follows:
By the data in first sampling module of the sampled data of each indirect dimension evolution incremental time in sampling time T
Point carries out sequential concatenation, arranges to reconstruct the first row of two-dimensional matrix, successively will be in the sampling time in (2N+1) a sampling module
Data point row for reconstruct two-dimensional matrix Nth row, to obtain required two-dimensional data matrix.
Compared to the prior art, technical solution of the present invention have it is following the utility model has the advantages that
1. the present invention is uncoupled the combination of module and echo train sampling module by ZS, the chemistry in final two-dimentional spectrogram
Displacement dimension only show pure chemistry displacement information, improves spectra resolution rate, alleviate complex sample detect in spectral peak is crowded asks
Topic, overcomes deficiency of the existing two dimension J spectral technology in practical application；Meanwhile the two-dimentional J that the technology obtains decomposes spectrum with phase sensitivity
Spectral model shows that the two-dimentional J for solving traditional absolute value mode decomposes the phase-modulation effect composed in experiment, further improves
Spectra resolution rate is of great significance for possessing the structure detection of complex sample of crowded spectral peak.
2. the present invention is uncoupled the flexible choice of π selective pulse time in module by ZS, for being coupled the shadow of artifact by force
Sound has certain inhibiting effect, is of great significance for the sample detection comprising being coupled system by force and signal assignment.
3. the present invention is uncoupled the use of slice selective gradient between module hollow by ZS, the office of conventional two-dimensional J spectral method is breached
It is sex-limited, the influence of coding direction (direction z) Magnetic field inhomogeneity is effectively eliminated, while in the spin theory at a slow speed for passing through sample
It is uneven to eliminate the direction xy sample, to obtain a high-resolution two dimension J spectrum in non-uniform magnetic field environment down-sampling, greatly expands
The application field of two-dimentional J spectrum.This method is suitable for conventional nuclear magnetic resonance chemical analyser, does not need any special hardware device, and
And be not necessarily to any special sample pretreatment process, it is simple and easy to do, for obtain complicated organic sample and biological tissue samples two
Dimension J decomposes spectrum and provides a kind of important means.
4. invention further provides a kind of applicable sensitivity to mention on the basis of basic super-resolution two dimension J spectral method
Phase sensitivity two dimension J spectral sensitivity is significantly increased by being based on the pulsed multi-channel sampling of polychrome in the high method of sampling
Energy.Theoretically, N times of sensitivity can be improved in N color pulse excitation.
Detailed description of the invention
Fig. 1 is that super-resolution nuclear magnetic resonance two dimension phase sensitivity J proposed by the present invention decomposes spectral method pulse sequence diagram；It is black in figure
Color Filled Rectangle item is the pulse of pi/2 non-selective radio frequency, and black hollow rectangle item is the pulse of π non-selective radio frequency, gaussian shape
Item is π selective pulse, and rectangular strip indicates dephasing gradient G1、G2、G3And space slice selective gradient GZ, T is each sampling module
Sampling time.
Fig. 2 is the one-dimensional hydrogen spectrum of routine obtained in the direction z Magnetic field inhomogeneity situation down-sampling, and sample is dissolved in deuterated acetone
Methacrylic acid fourth lipoprotein solution, sample concentration be 500 mMs every liter.Magnetic field inhomogeneity causes spectrogram line width to be about 400Hz.
Fig. 3 is in above-mentioned Magnetic field inhomogeneity, and the present invention proposes that super-resolution nuclear magnetic resonance two dimension phase sensitivity J decomposes spectrum
Method is applied to methacrylic acid fourth lipoprotein solution and obtains two dimension J decomposition spectrum, and wherein horizontal axis is chemical shift dimension, and the longitudinal axis is J coupling
Dimension.(embodiment 1)
Fig. 4 is the experiment proposed by the present invention for being applied to above-mentioned sample using the pulsed sensitivity Enhancement Method of polychrome
As a result (it is four color pulse excitations that example, which uses).This experiment is carried out under uniform magnetic field environment.Theoretically, compared to
Four times of spectrogram sensitivity can be improved in monochromatic pulses excitation, four color pulse excitations.(embodiment 2)
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method is present embodiments provided, following steps are included:
1) according to detection demand, prepare the solution example or biological tissue samples of detection, and ready test sample
It is fitted into nuclear magnetic resonance sample pipe.Then the sample cell is sent into the test chamber of nuclear magnetic resonance spectrometer；
2) emit radio-frequency pulse and gradient pulse sequence, the pulse train are by pi/2 non-selective excitation pulse, ZS is gone
Even module and echo train sampling module are constituted.According to timing, the ZS uncouple module include first dephasing gradient pulse, one
A non-selective pulse of π, second dephasing gradient pulse and apply one π selective pulse simultaneously with selective pulse
Space slice selective gradient, third dephasing gradient pulse.Wherein the direction of all gradient pulses is identical, and along Z-direction；Described
Echo train sampling module includes the sampling module for repeating 2N times, and each sampling module is non-selection by one section of sampling time T and a π
Property pulse composition.It is final to realize what sample chemical displacement and J were coupled by the collective effect of above-mentioned radio-frequency pulse and gradient pulse
Develop and separate, to show chemical shift and J coupling respectively on two independent dimensions (chemical shift peacekeeping J coupling dimension)
Information.
3) data sampling is carried out.The novel nuclear magnetic resonant impulse sequence of aforementioned present invention design is imported on nuclear magnetic resonance spectrometer
Column, and experiment parameter is set, data acquisition then is carried out to the NMR signal to be developed.
4) Data Post.After the completion of data acquisition, calls Data Post code to carry out data processing, can be obtained tool
Have to resist Magnetic field inhomogeneity and reduce the strong super-resolution nuclear magnetic resonance phase sensitivity two dimension J for being coupled artifact advantage and decomposes spectrum.
Step 2) ZS module of uncoupling is the phase (t that developed by spin echo1Non-selective pulse-the t of/2-π1/ 2),
One π selective pulse and the space slice selective gradient applied simultaneously with selective pulse form, wherein t1To tie up evolution indirectly
Time.Under the action of ZS uncouples module, excited nucleus is constant, coupling core (atomic nucleus with excitation nuclear phase coupling) 180 ° of overturning,
Therefore J coupling of the signal in sampling start time is met again, and only retains chemical shift evolution, shorter when the sampling time
When, it can be approximately zero that the J coupling of signal, which is developed,.With indirect dimension evolution time t1Increase, sampled.By each hits
Free damping signal in preceding T time carries out sequential concatenation, and carries out Fourier transformation to spliced signal, thus
A dimension (chemical shift dimension) in final two-dimentional spectrogram only shows pure chemistry displacement information, improves spectra resolution rate,
Alleviate the problem that spectral peak is crowded in complex sample detection.
The non-selective pulse of π in step 2) the echo train sampling module guarantees in the interval sampling before and after π pulse
Between the chemical shift evolution of sampled signal of 2T met again, only retain J coupling.Therefore in final two-dimentional spectrogram
Display J is coupled information to another dimension (J coupling dimension), to be applied to the measurement of the ownership and J coupling constant of chemical constituent.
Step 3) the experimental series are shown in that attached drawing 1, the experiment parameter include: direct dimension spectrum width SW, tie up spectrum width indirectly
SW1, the indirectly pulse width of dimension evolution incremental time number ni, π selective pulse and radio-frequency power, same with π selective pulse
When the space slice selective gradient G that appliesZ, dephasing gradient intensity G1、G2、G3And action time, sampling time T, sampling module repetition time
Number 2N, accumulative frequency nt.
The process of the step 4) data processing is as follows: (a) since the odd-times in only echo train sampling module samples mould
Block data collected are the useful data needed, during processing separate odd and even data, individually extract odd-times data
It is handled；It (b) is a two-dimensional data matrix by odd-times data reconstruction.Specific steps are as follows: when will each tie up evolution indirectly
Between increment sampled data first sampling module in data point in sampling time T carry out sequential concatenation, arrange as reconstruct two
The first row of matrix is tieed up, is successively reconstruct two-dimensional matrix by the data point row in the sampling time in (2N+1) a sampling module
Nth row, to obtain required two-dimensional data matrix；(c) two-dimensional Fourier transform is carried out to obtained 2-D data, and carried out
Phase modulation operation, the phase sensitivity two dimension J that can be obtained a super-resolution decompose spectrum.
The super-resolution two dimension J spectral method for the sensitivity enhancing that the present embodiment also proposes is sampled based on polychrome pulse excitation
It realizes.Its specific steps is substantially identical as above-mentioned basic super-resolution two dimension J spectral method, and specific difference is as follows:
π selective pulse in step 2) is the polychrome pulse for exciting multiple Frequency points, and needs generation more according to experiment
The pulse width and radio-frequency power of color pulse.(embodiment 2)
The present invention is proposed a kind of for accurate by the design of novel pulse sequence and the combination of data post processing method
The nuclear magnetic resonance phase sensitivity two dimension J for measuring J coupling constant and obtaining the super-resolution of molecular structure information decomposes spectrum.
A kind of above-mentioned super-resolution nuclear magnetic resonance phase sensitivity two dimension J for obtaining molecular structure information decomposes spectral method.
This method is uncoupled the collective effect of module and echo train sampling module based on ZS, is overcome existing two dimension J spectral technology and is actually being answered
With deficiency, effectively eliminate the influence of Magnetic field inhomogeneity on space slice selective gradient direction (direction z), can be uneven
High-resolution two dimension phase sensitivity J is obtained under magnetic field environment and decomposes spectrum, for the structural information for obtaining heterogeneous sample and biological tissue samples
Provide a kind of important means, so as to for sample Structural Identification and component analysis.This method improves conventional two-dimensional J points
The spectra resolution rate of spectrum unscrambling, the structure detection and signal assignment of the complex sample crowded for spectral peak are of great significance, significantly
Extend the application field of two-dimentional J spectrum.Meanwhile on the basis of monochromatic pulses exciting method, invention further provides based on more
The sensitivity Enhancement Method that the sampling of color pulse excitation is realized.
Embodiment 1 is that the present invention proposes that a kind of nuclear magnetic resonance phase sensitivity two dimension J of the super-resolution of monochromatic pulses excitation is decomposed
Spectral method (refers to Fig. 1-3).Embodiment 2 is the sensitivity enhancing proposed by the present invention realized based on the sampling of polychrome pulse excitation
Super-resolution two dimension J spectral method (refers to Fig. 4).
Sample used in example 1 and example 2 is dissolved in the methacrylic acid fourth lipoprotein solution of deuterated acetone, sample concentration
It is 500 mMs every liter.The instrument used is Varian 500MHz NMR spectrum view.To prove that this method resists magnetic field
The performance of inhomogeneities, the artificial direction tuningout z magnetic field during monochromatic pulses excitation experiment.It is proposed according to aforementioned present invention
Operating process is packed into laboratory sample, imports compiled pulse train as shown in Figure 1, corresponding experiment parameter is arranged.Particularly for
Sample used by this example, the design parameter of basic monochromatic pulses excitation experiment are provided that π selectivity monochromatic pulses
Time and radio-frequency power are respectively 20ms and 6dB；The slice selective gradient intensity G applied simultaneously with selective pulseZFor 0.73G/cm；
Dephasing gradient G1、G2、G3Intensity is respectively 11G/cm, -11G/cm, -22G/cm, action time 1.0ms；Directly tie up spectrum width SW
=10000Hz；Dimension spectrum width SW1=100Hz indirectly；Dimension evolution incremental time ni=70 indirectly；Each sampling module sampling time T
=20.0ms；Sampling module number of repetition 2N=160；The pulse train waiting time is 1s；Accumulative frequency nt=8.Entire experiment
Used time is about 15min.After the completion of data sampling, initial data is handled according to above-mentioned Data Post process, can be obtained
Two-dimentional phase sensitivity J as shown in Figure 3 decomposes spectrum, wherein the two-dimentional J spectrum longitudinal axis indicate the scalar coupling in detection molecules split merotype and
Corresponding coupling constant；Horizontal axis indicates nuclear chemical shift in different chemical environments.Therefore, which can be used as to test sample
Progress component analysis and Structural Identification an important evidence.π selective pulse in polychrome pulse excitation experiment in example 2
For polychrome pulse, burst length and radio-frequency power are respectively 20ms and 18dB, and the setting of remaining design parameter is tested with monochromatic pulses
It is identical.This experiment is carried out under uniform magnetic field environment.After the completion of data sampling, according to above-mentioned Data Post process pair
Initial data is handled, and experimental result is as shown in Figure 4.
Described above, only the present invention preferably implements example, cannot limit the scope of implementation of the present invention according to this.I.e. according to this
Equivalent changes and modifications made by patent of invention range and description, should still be within the scope of the present invention.
Claims (6)
1. a kind of super-resolution nuclear magnetic resonance two dimension J decomposes spectral method, it is characterised in that include the following steps:
1) sample is sent into the test chamber of nuclear magnetic resonance spectrometer；
2) emit radio-frequency pulse and gradient pulse sequence, the evolution and separation of sample chemical displacement and J coupling are realized, thus changing
Chemical shift and J coupling information are shown in displacement study peacekeeping J coupling dimension respectively；
The pulse train is by pi/2 non-selective excitation pulse, and uncouple module and echo train sampling module of ZS is constituted；
The ZS uncouple module include first dephasing gradient pulse, a non-selective pulse of π, second dephasing gradient arteries and veins
Punching, a π selective pulse and the space slice selective gradient applied simultaneously with selective pulse, third dephasing gradient pulse；
Wherein the direction of all gradient pulses is identical, and along Z-direction；
The echo train sampling module includes repeating 2N sampling module, each sampling module by one section of sampling time T with
The non-selective pulse composition of one π；
3) data sampling and Data Post are carried out, acquisition is counted with resistance Magnetic field inhomogeneity and reduction and is coupled artifact advantage by force
Super-resolution nuclear magnetic resonance phase sensitivity two dimension J decomposes spectrum.
2. a kind of super-resolution nuclear magnetic resonance two dimension J according to claim 1 decomposes spectral method, it is characterised in that: step
4) process of the data processing is as follows:
(a) since the odd-times sampling module data collected in only echo train sampling module are the useful data needed,
Odd and even data is separated in treatment process, odd-times data is individually extracted and is handled；
It (b) is a two-dimensional data matrix by odd-times data reconstruction；
(c) two-dimensional Fourier transform is carried out to obtained 2-D data, and carries out phase modulation operation, can be obtained a super-resolution
Phase sensitivity two dimension J decompose spectrum.
3. a kind of super-resolution nuclear magnetic resonance two dimension J according to claim 2 decomposes spectral method, it is characterised in that: specific
Step are as follows: the specific practice of the step b of data processing are as follows:
Data in first sampling module of the sampled data of each indirect dimension evolution incremental time in sampling time T are clicked through
Row sequential concatenation is arranged to reconstruct the first row of two-dimensional matrix, successively by the number in the sampling time in (2N+1) a sampling module
Strong point row is the Nth row of reconstruct two-dimensional matrix, to obtain required two-dimensional data matrix.
4. a kind of super-resolution nuclear magnetic resonance two dimension J decomposes spectral method, it is characterised in that include the following steps:
1) sample is sent into the test chamber of nuclear magnetic resonance spectrometer；
2) emit radio-frequency pulse and gradient pulse sequence, the evolution and separation of sample chemical displacement and J coupling are realized, thus changing
Chemical shift and J coupling information are shown in displacement study peacekeeping J coupling dimension respectively；
The pulse train is by pi/2 non-selective excitation pulse, and uncouple module and echo train sampling module of ZS is constituted；
The ZS uncouple module include first dephasing gradient pulse, a non-selective pulse of π, second dephasing gradient arteries and veins
Punching, a π selective pulse and the space slice selective gradient applied simultaneously with selective pulse, third dephasing gradient pulse；
Wherein the direction of all gradient pulses is identical, and along Z-direction；A π selective pulse is the more of the multiple Frequency points of excitation
Color pulse；
The echo train sampling module includes repeating 2N sampling module, each sampling module by one section of sampling time T with
The non-selective pulse composition of one π；
3) data sampling and Data Post are carried out, acquisition is counted with resistance Magnetic field inhomogeneity and reduction and is coupled artifact advantage by force
Super-resolution nuclear magnetic resonance phase sensitivity two dimension J decomposes spectrum.
5. a kind of super-resolution nuclear magnetic resonance two dimension J according to claim 4 decomposes spectral method, it is characterised in that: step
4) process of the data processing is as follows:
(a) since the odd-times sampling module data collected in only echo train sampling module are the useful data needed,
Odd and even data is separated in treatment process, odd-times data is individually extracted and is handled；
It (b) is a two-dimensional data matrix by odd-times data reconstruction；
(c) two-dimensional Fourier transform is carried out to obtained 2-D data, and carries out phase modulation operation, can be obtained a super-resolution
Phase sensitivity two dimension J decompose spectrum.
6. a kind of super-resolution nuclear magnetic resonance two dimension J according to claim 5 decomposes spectral method, it is characterised in that: specific
Step are as follows: the specific practice of the step b of data processing are as follows:
Data in first sampling module of the sampled data of each indirect dimension evolution incremental time in sampling time T are clicked through
Row sequential concatenation is arranged to reconstruct the first row of two-dimensional matrix, successively by the number in the sampling time in (2N+1) a sampling module
Strong point row is the Nth row of reconstruct two-dimensional matrix, to obtain required two-dimensional data matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910542558.5A CN110361680A (en) | 2019-06-21 | 2019-06-21 | A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910542558.5A CN110361680A (en) | 2019-06-21 | 2019-06-21 | A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110361680A true CN110361680A (en) | 2019-10-22 |
Family
ID=68217490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910542558.5A Pending CN110361680A (en) | 2019-06-21 | 2019-06-21 | A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110361680A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114487954A (en) * | 2022-04-14 | 2022-05-13 | 中国科学院精密测量科学与技术创新研究院 | Multichannel receiving and transmitting NMR method for accurately measuring field intensity and distribution of electromagnet |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030160616A1 (en) * | 2002-02-22 | 2003-08-28 | Kenji Asano | MRI apparatus |
| CN103472420A (en) * | 2013-09-30 | 2013-12-25 | 厦门大学 | Method for obtaining high resolution nuclear magnetic resonance heteronuclear spectrogram in unknown spatial distribution magnetic field |
| CN106814337A (en) * | 2017-01-21 | 2017-06-09 | 厦门大学 | A kind of nuclear magnetic resonance transverse relaxation measuring method without the interference of J coupling effects |
| CN106841270A (en) * | 2017-01-21 | 2017-06-13 | 厦门大学 | A kind of method for obtaining nuclear magnetic resonance two dimension phase sensitivity J spectrums |
| CN106872506A (en) * | 2017-03-15 | 2017-06-20 | 厦门大学 | A kind of supper-fast nuclear magnetic resonance two dimension J spectral methods for resisting non-uniform magnetic field |
| CN107505349A (en) * | 2017-09-27 | 2017-12-22 | 厦门大学 | A kind of nuclear magnetic resonance for differentiating true and false honey is uncoupled hydrogen spectral method |
| CN107894436A (en) * | 2017-10-30 | 2018-04-10 | 厦门大学 | A kind of fast two-dimensional J spectral methods applied to non-uniform magnetic field |
| CN108169273A (en) * | 2017-12-28 | 2018-06-15 | 厦门大学 | Realize that the twodimensional magnetic resonance monomer element localization J of pure absorption line shape decomposes spectral method |
-
2019
- 2019-06-21 CN CN201910542558.5A patent/CN110361680A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030160616A1 (en) * | 2002-02-22 | 2003-08-28 | Kenji Asano | MRI apparatus |
| CN103472420A (en) * | 2013-09-30 | 2013-12-25 | 厦门大学 | Method for obtaining high resolution nuclear magnetic resonance heteronuclear spectrogram in unknown spatial distribution magnetic field |
| CN106814337A (en) * | 2017-01-21 | 2017-06-09 | 厦门大学 | A kind of nuclear magnetic resonance transverse relaxation measuring method without the interference of J coupling effects |
| CN106841270A (en) * | 2017-01-21 | 2017-06-13 | 厦门大学 | A kind of method for obtaining nuclear magnetic resonance two dimension phase sensitivity J spectrums |
| CN106872506A (en) * | 2017-03-15 | 2017-06-20 | 厦门大学 | A kind of supper-fast nuclear magnetic resonance two dimension J spectral methods for resisting non-uniform magnetic field |
| CN107505349A (en) * | 2017-09-27 | 2017-12-22 | 厦门大学 | A kind of nuclear magnetic resonance for differentiating true and false honey is uncoupled hydrogen spectral method |
| CN107894436A (en) * | 2017-10-30 | 2018-04-10 | 厦门大学 | A kind of fast two-dimensional J spectral methods applied to non-uniform magnetic field |
| CN108169273A (en) * | 2017-12-28 | 2018-06-15 | 厦门大学 | Realize that the twodimensional magnetic resonance monomer element localization J of pure absorption line shape decomposes spectral method |
Non-Patent Citations (1)
| Title |
|---|
| 法拉: "《脉冲核磁共振波谱学导论 第1版》", 30 June 1989 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114487954A (en) * | 2022-04-14 | 2022-05-13 | 中国科学院精密测量科学与技术创新研究院 | Multichannel receiving and transmitting NMR method for accurately measuring field intensity and distribution of electromagnet |
| CN114487954B (en) * | 2022-04-14 | 2022-07-01 | 中国科学院精密测量科学与技术创新研究院 | Multichannel transmitting-receiving NMR method for accurately measuring field intensity and distribution of electromagnet |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106093099B (en) | A kind of method for obtaining high-resolution two dimension J and decomposing spectrum | |
| CN107144591B (en) | A method of measurement is independently with nuclear spin to indirect coupling mode | |
| CN106841270B (en) | A kind of method for obtaining nuclear magnetic resonance two dimension phase sensitivity J spectrums | |
| CN106872506B (en) | A kind of supper-fast nuclear magnetic resonance two dimension J spectral methods for resisting non-uniform magnetic field | |
| CN106814337B (en) | A kind of nuclear magnetic resonance transverse relaxation measurement method of no J coupling effect interference | |
| CN105158289B (en) | A kind of magnetic resonance detection method for biological tissue | |
| CN105651803B (en) | A kind of nuclear magnetic resonance two-dimensional diffusion sequence spectral method for any magnetic field environment | |
| CN103885013B (en) | Under a kind of non-uniform magnetic field, obtain the method that nuclear magnetic resonance two dimension J divides spectrum unscrambling | |
| CN103941204B (en) | A kind of method obtaining high resolution nuclear magnetic resonance Three Dimensional Spectrum under non-uniform magnetic field | |
| CN106645255B (en) | A kind of monomer element localization One-Dimensional Pure chemical shift nuclear magnetic resonance spectral method | |
| CN103744042B (en) | The method of nuclear magnetic resonance spin echo correlated spectroscopy is obtained under non-uniform magnetic field | |
| CN107015181A (en) | A kind of method that proton longitudinal relaxation time is measured under non-uniform magnetic field | |
| CN107907558B (en) | A kind of nuclear magnetic resonance diffusion spectral method based on purifying displacement study | |
| CN104237820A (en) | Method for acquiring two-dimensional J-resolved spectroscopy of magnetic resonance by single sweeping | |
| JP5364954B2 (en) | NMR measurement method | |
| Wu et al. | Ultrafast 2D COSY with constant-time phase-modulated spatial encoding | |
| CN103472420A (en) | Method for obtaining high resolution nuclear magnetic resonance heteronuclear spectrogram in unknown spatial distribution magnetic field | |
| CN110361680A (en) | A kind of super-resolution nuclear magnetic resonance two dimension J decomposition spectral method | |
| CN103645453B (en) | A kind of method obtaining the monomer element one dimension localization spectrum eliminating scalar coupling modulation | |
| CN107894436B (en) | A kind of fast two-dimensional J spectral method applied to non-uniform magnetic field | |
| CN105928965A (en) | Method for restraining nuclear magnetic resonance spectrum sampling truncation false peaks | |
| CN112132028A (en) | Method for obtaining high-quality nuclear magnetic resonance pure chemical shift spectrum | |
| CN105004748B (en) | A kind of method that the relevant spectrogram of two dimensional NMR is obtained under non-uniform magnetic field | |
| CN109884107B (en) | Method for measuring same-core indirect coupling network | |
| Zhang et al. | Fast high-resolution 2D NMR spectroscopy in inhomogeneous fields via Hadamard frequency encoding and spatial encoding |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191022 |