CN110359276A - Poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush and its preparation method and application - Google Patents
Poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush and its preparation method and application Download PDFInfo
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- CN110359276A CN110359276A CN201910691794.3A CN201910691794A CN110359276A CN 110359276 A CN110359276 A CN 110359276A CN 201910691794 A CN201910691794 A CN 201910691794A CN 110359276 A CN110359276 A CN 110359276A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M14/00—Graft polymerisation of monomers containing carbon-to-carbon unsaturated bonds on to fibres, threads, yarns, fabrics, or fibrous goods made from such materials
- D06M14/08—Graft polymerisation of monomers containing carbon-to-carbon unsaturated bonds on to fibres, threads, yarns, fabrics, or fibrous goods made from such materials on to materials of synthetic origin
- D06M14/12—Graft polymerisation of monomers containing carbon-to-carbon unsaturated bonds on to fibres, threads, yarns, fabrics, or fibrous goods made from such materials on to materials of synthetic origin of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M14/14—Polyesters
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/16—Synthetic fibres, other than mineral fibres
- D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M2101/32—Polyesters
Abstract
Poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush of the present invention and its preparation method and application, the present invention are used for Thermosensitive Material Used for Controlled Releasing of Medicine.The material uses polyisopropyl acrylamide, decanedioic acid and glycerine for raw material, preparation method is first with decanedioic acid and glycerine at 160 DEG C, poly- decanedioic acid glyceride is synthesized under nitrogen environment, poly- decanedioic acid glyceride is passed through after electrospinning later, by polyisopropyl acrylamide molecular brush by ATRP reactive grafting in PGS fiber surface, preparation has temperature sensitive fiber, makes the fiber solidifying molding of PGS finally by heat cure.Present invention process is simple, the micro/nano fibrous membrane material large specific surface area of preparation, and flexibility is good, tensile strength is high, and the molecule brush configuration with temperature switch effectively controls the control and release of drug, has important application value in terms of biological support and medicament slow release.
Description
Technical field
The invention belongs to bio-medical organizational project medicament slow release fields, and in particular to polyisopropyl acrylamide
(PNIPAM) preparation of molecular brush PGS base tunica fibrosa.
Background technique
The development of organizational engineering provides a kind of technological means of regeneration, i.e., will have good biocompatibility,
Degradability and absorbable biomaterial (bracket) mix according to a certain percentage with cell, make cell adhesion in biomaterial
Cell-material composite is formed on (bracket)；By the tissue of the compound implanting to human body or lesions position, with biological material
Material is gradually degraded and absorbs in vivo, and the cell of implantation is constantly proliferated in vivo and extracellular matrix secretion, ultimately forms phase
The tissue or organ answered, to achieve the purpose that repair wound and Reconstruction of The Function.This material is tissue engineering bracket material, is changed
The treatment mode for having become surgery traditional " with wound repair wound ", marches toward the new stage of hurtless measure reparation.
In recent years, during high molecular material is applied with being widely used in bio-medical material, and medicament slow release macromolecule material
Material is popular research topic.Medicament slow release is that pharmaceutical activity molecule is combined (compound, packing etc.) in polymer carrier
Get up, launch in bioactivity body by diffusion, the modes such as infiltration, when pharmaceutical activity molecule is to be single concentration and is lasting
Between release, to give full play to the curative effect of medicine.The delivery mode of this drug can efficiently control the dosage of drug, drop
The toxic side effect of low drug reduces drug resistance, improves the stability and utilization rate of drug；The targeting of drug additionally may be implemented
Conveying reduces medicining times.The controlled release mechanism of different high molecular materials, drug is different, therefore bears in tissue stent material
Drug is carried to act on injury, can effectively accelerate the recovery of injury.
Biodegradable polymer has huge potentiality in the every field of bioengineering, as organizational project, drug are defeated
It send, and induction in vivo.Since many biomedical devices are implanted in the mechanically dynamic environments of human body, so implantation material is necessary
Form is maintained in the case where no mechanical stimulus and is recovered from various modifications.In many cases, these implantation materials
Matrix and bracket preferably be made of biodegradable polymer, property be similar to natural extracellular matrix (ECM), one
Soft, the tough and tensile and elastic protein network of kind provides mechanical stability and structural intergrity for tissue and organ.Therefore, one
The soft biodegradable elastomer of kind can recover from relatively large deformation, this is conducive to keep implantation material
Normal function, without generating mechanical stimulus to host.Master is to be applied hydrogel, Elastin peptide and poly-hydroxy fatty acid
Ester (PHA).
Poly- decanedioic acid glyceride (PGS) is that a kind of good toughness, cost performance be high and material with good cell compatibility,
Microstructure is similar to vulcanizate, because polymer long-chain carries out crosslinking and its hydrogen bond action forms three-dimensional structure, with water
Gel is compared, and has stronger toughness；It compares with Elastin peptide with disimmune, no cytotoxicity and cheap excellent
Point；With deformation recovery capability more larger range of than PHA.In addition, PGS material has loads Large molecule active drug well
Ability, internal cross-linked structure and polar group can provide good attachment site for active drug molecule.PNIPAM is one
Kind has the high molecular material of responsive to temperature type, and when being lower than the temperature of LUST, the expansion of molecule interchain has hydrophily, and is higher than
When LUST, the hydrogen bond action of molecule interchain is connected to together.As a kind of switching material of temperature-responsive, widely answered
For in medicament slow release, water-oil separating, memory or temperature-sensitive switch material.
Summary of the invention
The purpose of the present invention is drug release in organizational project there are aiming at the problem that, a kind of poly- isopropyl acrylamide is provided
Amine (PNIPAM) molecular brush PGS base tunica fibrosa and preparation method thereof, the present invention utilize electrostatic spinning technique and ATRP reaction mechanism
Polyisopropyl acrylamide (PNIPAM) molecular brush PGS base fiber film material is prepared in the method combined with heat cure, tool
There is the performance of excellent adsorption activity macromolecular, can be used as pharmaceutical carrier.
The technical solution adopted by the invention is as follows:
The poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush, the non-1-10 μ of fibre diameter in tunica fibrosa
M, fiber surface are grafted with the polyisopropyl acrylamide strand of certain length, the polyisopropyl acrylamide polymerization degree n
=200-1000.
The preparation method of the poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush, comprising the following steps:
(1) decanedioic acid and glycerine are added in three-necked flask with the molar ratio of 1:1, by mechanical stirring, in 160 DEG C of temperature
Degree is lower to react 10h, and nitrogen stream is blown in reaction process, obtains PGS prepolymer；
(2) PGS prepolymer and TEA are dissolved in anhydrous methylene chloride, stream blows 30min under a nitrogen, by a certain amount of BiBB
It is dropwisely added in solution, reaction for 24 hours, keeps nitrogen atmosphere, magnetic agitation, after reaction, carbon is used in aqueous precipitation filtering in the process
Sour hydrogen sodium solution and saturated sodium chloride solution are washed three times, after dry, obtained macromolecular photoinitiator PGS-Br, are saved under nitrogen；
(3) PGS-Br obtained above is mixed with PGS and is dissolved in DCM/DMF in the mixed solvent, a certain amount of PLA is added and helps
It spins, configures certain density spinning solution, electrospinning in syringe is added, fiber is collected on roller receiver, is put at room temperature
12h is set, solvent is made sufficiently to volatilize, obtains tunica fibrosa；
(4) obtained tunica fibrosa is placed in flask, catalyst CuBr/CuBr2 is added in another flask and leads to nitrogen
NIPAM monomer and PMDETA ligand are transferred in the flask containing tunica fibrosa, in freeze-thaw nitrogen by 30min with syringe
After replacement cycles, above-mentioned catalyst is rapidly joined in the flask containing tunica fibrosa and is reacted, afterwards plus water terminate reaction, and spend from
Sub- water washes off monomer or chain substance on film；
(5) finally obtained tunica fibrosa is placed in a vacuum drying oven, is solidified under vacuum condition.
Preferably, PGS:BiBB:TEA molar ratio is 1:1.2:1.25 in the step (2).
Preferably, PLA concentration is 8wt% in the step (3), PGS-Br/PGS molar ratio is 1:3-7, PGS concentration
For 25-35wt%；Electrospinning environment temperature is 25 DEG C, humidity 15%, and high voltage power supply output voltage is 20KV, spinning solution flow velocity
For 0.6mL/h, distance is 20cm between reception device and spinning nozzle.
Preferably, monomer NIPAM is excessive in the step (4), reaction time 30-60min.
Preferably, curing time is for 24 hours in the step (5).
The application of the poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush, as answering for pharmaceutical carrier
With.
Polyisopropyl acrylamide (PNIPAM) molecular brush PGS base fiber film material answering in medicament slow release of the invention
With, with before pertinent literature report compared with, have the advantage that
(1) polyisopropyl acrylamide of the invention (PNIPAM) molecular brush PGS base fiber film material, cannot in view of PGS
Injection forms fiber in electrostatic field, by electrostatic spinning, PGS can be made to have a fibre morphology under spinning-aid agent effect, and one
Aspect increases the fibre forming property of PGS, effectively increases the specific surface area of material, and on the other hand, after PGS is at fibre, surface area increases,
Be conducive to the generation of PNIPAM, more PNIPAM molecular brush can be formed in fiber surface, improve Drug loading capacity；PNIPAM
Forming amount on the surface PGS is excessive to be unfavorable for, and carries that medicine space is small, is unfavorable for carrying the progress of medicine, formation of the PNIPAM on the surface PGS
It is big to measure too small meeting drugloading rate, but the burst release of drug can be generated；
(2) polyisopropyl acrylamide of the invention (PNIPAM) molecular brush PGS base fiber film material has preferable thin
Born of the same parents' compatibility can effectively increase sticking, migrate and being proliferated for cell；
(3) polyisopropyl acrylamide of the invention (PNIPAM) molecular brush PGS base fiber film material, by PGS as interior
Nuclear material can not only increase the flexibility of material, because of the performance of its excellent adsorption activity macromolecular, to enhance drug suction
Attached performance, and the release of drug can be effectively controlled by the cladding of outer layer PNIPAM by temperature, when being lower than LUST,
The PNIPAM of outer layer provides the release channel of macromolecular, and channel is closed when being higher than LUST, efficiently controls drug release.
Detailed description of the invention
Fig. 1 is that electrostatic spinning of the present invention tests easy device figure；
Fig. 2 is the nucleus magnetic hydrogen spectrum figure and infrared spectrogram of the macromole evocating agent PGS-Br of this experiment synthesis；
Fig. 3 is the scanning electron microscope (SEM) photograph of this experiment PGS base tunica fibrosa.
Specific embodiment
The synthetic example of macromolecular photoinitiator PGS-Br:
(1) decanedioic acid and glycerine are added in three-necked flask with the molar ratio of 1:1, by mechanical stirring, in 160 DEG C of temperature
Degree is lower to react 10h, and nitrogen stream is blown in reaction process, obtains PGS prepolymer；
(2) PGS prepolymer and TEA are dissolved in anhydrous methylene chloride, stream blows 30min under a nitrogen, by a certain amount of BiBB
It is dropwisely added in solution, reaction for 24 hours, keeps nitrogen atmosphere, magnetic agitation, after reaction, carbon is used in aqueous precipitation filtering in the process
Sour hydrogen sodium solution and saturated sodium chloride solution are washed three times, after dry, obtained macromolecular photoinitiator PGS-Br, are saved under nitrogen；
Wherein PGS:BiBB:TEA molar ratio is 1:1.2:1.25.Characterization by the nuclear magnetic spectrogram carried out to product, at 1.94ppm
Appearance-CH3Peak, illustrate on PGS to cause group in successfully grafting, be successfully prepared macromole evocating agent.
Embodiment 1
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 35% of 1:4, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 30min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 2
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 35% of 1:3, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 30min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 3
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 35% of 1:7, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 30min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 4
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 30% of 1:4, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 30min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 5
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 25% of 1:4, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 30min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 6
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 35% of 1:4, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 60min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 7
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 25% of 1:3, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 60min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Embodiment 8
By PGS-Br and PGS with the DCM/DMF solution of the PGS/PGS-Br of the proportional arrangement 25% of 1:7, and configure 8wt%
PLA solution.Sufficiently dissolution under magnetic stirring, is added in syringe, is connected to syringe needle, is 20KV in high-voltage electricity, flow velocity is
0.6mL/h, receiving distance is spinning under conditions of 20cm, and tunica fibrosa is collected on roller；
Tunica fibrosa places 12h, volatile residue solvent at room temperature；ATRP reaction unit is added in tunica fibrosa afterwards, is added single
Body NIPAM, ligand PMEDTA, freeze-thaw circulation water removal deoxygenation, are added catalyst CuBr/CuBr2, 60min is reacted, water is added
Reaction is terminated, and rinses tunica fibrosa with deionized water；
Finally tunica fibrosa is put into 120 DEG C of vacuum oven and is solidified for 24 hours.
Application Example
Tunica fibrosa after above-mentioned spinning: being first soaked in the solution containing drug by sustained release experiment, will using deionized water
The drug of fiber surface is washed off, and after surface grafting molecular brush, it is carried out medicine controlled releasing experiment in PBS solution.
In vitro cell experiment: in vitro experiment, seeding cells on tunica fibrosa, detection cell adhesion, migration and increasing
Situation is grown, with the biological safety of test material.
Claims (7)
1. the poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush, it is characterised in that: fiber is straight in tunica fibrosa
Non- 1-10 μm of diameter, fiber surface is grafted with the polyisopropyl acrylamide strand of certain length, the poly- isopropyl acrylamide
Amine polymerization degree n=200-1000.
2. the preparation method of the poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush, it is characterised in that including with
Lower step:
(1) decanedioic acid and glycerine are added in three-necked flask with the molar ratio of 1:1, through mechanical stirring, at a temperature of 160 DEG C
10h is reacted, nitrogen stream is blown in reaction process, obtains PGS prepolymer；
(2) PGS prepolymer and TEA are dissolved in anhydrous methylene chloride, stream blows 30min under a nitrogen, dropwise by a certain amount of BiBB
Ground is added in solution, and reaction for 24 hours, keeps nitrogen atmosphere, magnetic agitation, after reaction, bicarbonate is used in aqueous precipitation filtering in the process
Sodium solution and saturated sodium chloride solution are washed three times, after dry, obtained macromolecular photoinitiator PGS-Br, are saved under nitrogen；
(3) PGS-Br obtained above is mixed with PGS and is dissolved in DCM/DMF in the mixed solvent, a certain amount of PLA is added and helps spinning,
Certain density spinning solution is configured, electrospinning in syringe is added, fiber is collected on roller receiver, is placed at room temperature
12h makes solvent sufficiently volatilize, and obtains tunica fibrosa；
(4) obtained tunica fibrosa is placed in flask, catalyst CuBr/CuBr2 is added in another flask and leads to nitrogen 30min,
NIPAM monomer and PMDETA ligand are transferred in the flask containing tunica fibrosa with syringe, followed in the displacement of freeze-thaw nitrogen
After ring, above-mentioned catalyst is rapidly joined in the flask containing tunica fibrosa and is reacted, afterwards plus water terminates reaction, and is washed with deionized water
Fall the monomer or chain substance on film；
(5) finally obtained tunica fibrosa is placed in a vacuum drying oven, is solidified under vacuum condition.
3. preparation method according to claim 2, it is characterised in that: PGS:BiBB:TEA molar ratio in the step (2)
For 1:1.2:1.25.
4. preparation method according to claim 2, it is characterised in that: in the step (3), PLA concentration is 8wt%,
PGS-Br/PGS molar ratio is 1:3-7, and PGS concentration is 25-35wt%；Electrospinning environment temperature is 25 DEG C, humidity 15%, high pressure
Electric power output voltage is 20KV, and spinning solution flow velocity is 0.6mL/h, and distance is 20cm between reception device and spinning nozzle.
5. preparation method according to claim 2, it is characterised in that: monomer NIPAM is excessive in the step (4), reaction
Time is 30-60min.
6. preparation method according to claim 2, it is characterised in that: curing time is for 24 hours in the step (5).
7. the application of the poly- decanedioic acid glycerol ester group tunica fibrosa of polyisopropyl acrylamide molecular brush prepared by claim 1,
It is characterized in that: the application as pharmaceutical carrier.
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Cited By (2)
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|---|---|---|---|---|
| CN111719243A (en) * | 2020-06-09 | 2020-09-29 | 紫罗兰家纺科技股份有限公司 | PGS/SF electrospun membrane and preparation method thereof |
| CN112695411A (en) * | 2020-12-28 | 2021-04-23 | 浙江理工大学 | Preparation method of temperature response type nanofiber dressing |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111719243A (en) * | 2020-06-09 | 2020-09-29 | 紫罗兰家纺科技股份有限公司 | PGS/SF electrospun membrane and preparation method thereof |
| CN111719243B (en) * | 2020-06-09 | 2022-03-01 | 紫罗兰家纺科技股份有限公司 | PGS/SF electrospun membrane and preparation method thereof |
| CN112695411A (en) * | 2020-12-28 | 2021-04-23 | 浙江理工大学 | Preparation method of temperature response type nanofiber dressing |
| CN112695411B (en) * | 2020-12-28 | 2022-07-01 | 浙江理工大学 | Preparation method of temperature response type nanofiber dressing |
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