CN110346193A - A kind of organization chip and cell line chip of observation of cell signal conduction path - Google Patents
A kind of organization chip and cell line chip of observation of cell signal conduction path Download PDFInfo
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- CN110346193A CN110346193A CN201910661637.8A CN201910661637A CN110346193A CN 110346193 A CN110346193 A CN 110346193A CN 201910661637 A CN201910661637 A CN 201910661637A CN 110346193 A CN110346193 A CN 110346193A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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Abstract
The invention discloses the organization chips and cell line chip of a kind of observation of cell signal conduction path, including being sliced body, biopsy cavity is equipped in the slicer body, clamping device of rising progressively is equipped in the biopsy cavity, chip wax stone is placed on the clamping device of rising progressively, side is symmetrically equipped with baffle before and after the clamping device of rising progressively, the baffle lower end is fixedly arranged in the biopsy cavity, rotary shaft is equipped on rear side of the clamping device of rising progressively, described rotary shaft or so is installed with fixed link, blade is fixedly installed between the fixed link, the present invention is sliced chip wax stone by the movement of blade, so that the integrity degree during chip block sections is high, improve the qualification rate of organization chip obtained, secondly, organization chip obtained, which is placed directly in cold water, to be unfolded, continuity operates the structural stability that organization chip can be improved.
Description
Technical field
The present invention relates to organization chip preparation technical field, specially a kind of tissue core of observation of cell signal conduction path
Piece and cell line chip.
Background technique
The existing organization chip for observation of cell signal conduction path is by first preparing chip wax stone, thereafter to tissue
Wax stone is sliced and is made, wherein the slicer of existing organization chip preparation, chip wax stone is clamped in liftable
In clamping device, blade position is fixed, and organization chip is made under blade effect in the lifting of chip wax stone, and organization chip is directly attached
On blade front end face, during this, due to the poor toughness of paraffin embedded tissues, to ensure chip wax stone during the work time complete
Whole property, the region for needing clamping device to clamp chip wax stone is big, then be sliced the later period can not to clamp position be sliced, cause core
The waste of piece wax stone, and organization chip obtained is easily adhered to each other on blade, is not easy to the completion separation of organization chip,
Secondary, organization chip, which cannot be placed directly in cold water, to be unfolded, and pilot process easily causes the fragmentation and thawing of organization chip.
For this reason, it is necessary to further improve organization chip slicer in the prior art, to improve organization chip
Integrality and slice efficiency.
Summary of the invention
The purpose of the present invention is to provide the organization chips and cell line chip of a kind of observation of cell signal conduction path, use
In overcoming drawbacks described above in the prior art.
The organization chip and cell line chip of a kind of observation of cell signal conduction path according to the present invention, including slicer
Body, the slicer body is interior to be equipped with biopsy cavity, is equipped with clamping device of rising progressively in the biopsy cavity, puts on the clamping device of rising progressively
It is equipped with chip wax stone, side is symmetrically equipped with baffle before and after the clamping device of rising progressively, and the baffle lower end is fixedly arranged on the slice
Intracavitary, described rise progressively is equipped with rotary shaft on rear side of clamping device, and described rotary shaft or so is installed with fixed link, between the fixed link
It is fixedly installed with blade, conveying device is equipped on the left of the biopsy cavity, the rotary shaft left end and the conveying device power connect
It connects, the conveying device drives the rotary shaft back-and-forth motion to be sliced the chip wax stone, on the downside of the conveying device
Equipped with power transmission, the power transmission is connected by power with the conveying device and the clamping device of rising progressively, institute
It states and is equipped with power connector on front side of conveying device, be equipped with lifting device on the downside of the power connector, before the biopsy cavity
Side is equipped with cold water cavity, is equipped with water tank in the cold water cavity, the water tank is connected by power with the lifting device, sets in the water tank
There is cavity, the cold water cavity right end wall is equipped with the water pipe of circulation, is equipped with water supply pump in the water pipe, when the conveying device triggers
When the power connector works, then the lifting device can drive the water tank to go up and down, after the slice on the blade
The chip wax stone place in the cavity, make under the work of the water supply pump water in the cold water cavity from the right side to
Left flowing, then the chip wax stone flows to cold water cavity inner cold water drift and opens, and with subsequent operation.
Further technical solution, the chip wax stone are made of numerous tissue core proper alignments, wherein tissue core be
It acquires and gets from the donor tissue wax stone with case research, the acceptor wax block of blank is equipped with numerous and tissue core quantity
The tissue core of extraction is placed in the hole of acceptor wax block by corresponding hole, so that the chip wax stone is formed, the chip wax stone
Organization chip is formed after sectioning.
Further technical solution, the clamping device of rising progressively includes gripping block, and clamping slot, institute are equipped in the gripping block
It states rotation in clamping slot and is equipped with two-way lead screw, be threaded with gripping block on the two-way lead screw, the chip wax stone is located at institute
It states between gripping block, is communicated with the outside world on the left of the biopsy cavity and be equipped with connection chamber, the intracavitary sliding of connection is equipped with connection block, institute
Two-way lead screw left end is stated through the connection block and stretches to the external world, the two-way lead screw passes through bearing rotation with the block that is connected to
Connection, the two-way lead screw left end are installed with adjusting block, communicate in the biopsy cavity right end wall and are equipped with slot of rising progressively, the slot of rising progressively
Interior rotation is equipped with threaded shaft, and the gripping block right end is threadedly engaged with the threaded shaft to be connect, phase on the upside of the slot of rising progressively
Logical to be equipped with engagement chamber, it is intracavitary and be installed with first bevel gear that the engagement is protruded into the threaded shaft upper end, the first cone tooth
Wheel right end engagement, which is connected with, resets bevel gear, and the reset bevel gear right side is installed with reset shaft, and the reset shaft is right
End protrudes into the external world and is installed with reset switch block, and the first bevel gear rear end engagement is connected with second bevel gear, second cone
Gear does not engage with the reset bevel gear, is installed with rotary gear shaft at the second bevel gear axle center, after the rotary gear shaft
End is connected by power with the power transmission；The distance between described gripping block is adjusted in the adjusting block rotation, to press from both sides
The tight chip wax stone, the chip wax stone by and slice it is primary after, the gripping block, which rises, drives the chip wax stone to rise
Certain distance, this distance are equal to the thickness of required organization chip.
Further technical solution, the conveying device include conveyor chamber, and rotation is equipped with rotary screw rod in the conveyor chamber,
It is threaded with movable block on the rotary screw rod, is equipped with swivelling chute in the movable block, the rotary shaft left end extends to institute
It states in swivelling chute and the rotary shaft and swivelling chute left end wall is rotatablely connected, in the rotary shaft in the swivelling chute
It is installed with rotating wheel, the rotating wheel upper end is installed with shifting block, is installed between the rotating wheel lower end and the conveyor chamber multiple
Position spring, the conveyor chamber upper end wall is fixed there are four fixed block, and the movable block drives the rotary shaft to move forward, can lead to
The blade is crossed by the chip block sections, with the continued advancement of the movable block, in the fixed block and the shifting block
Mating reaction under so that the rotating wheel drives the blade reciprocation to swing, then the organization chip on the blade falls into institute
It states in cavity, communicates on front side of the conveyor chamber equipped with drive cavity, the drive cavity front bulkhead is installed with engine, the rotation silk
Bar front end is connected by power in the engine, is installed with third hand tap gear on the rotary screw rod in the drive cavity, institute
It states the engagement of third hand tap gear left end and is connected with the 4th bevel gear, the 4th bevel gear left side is installed with mandrel, the mandrel
Left end is connected by power with the power connector.
Further technical solution, the power transmission include communicated with the conveyor chamber unidirectionally turn chamber, it is described
Unidirectionally turn intracavitary rotation and be equipped with outer runner, the outer runner engages connection, list in the outer runner with the movable block lower end surface
It is connected with handle ratchet wheel to engagement, described unidirectionally to turn to communicate on the left of chamber equipped with connecting cavity, rotation is equipped with the 5th cone in the connecting cavity
Gear, the 5th bevel gear and the handle ratchet wheel are connected, and the 5th bevel gear rear end engagement is connected with the 6th bevel gear, institute
It states and is installed with connecting shaft at the 6th bevel gear axle center, it is dynamic by driving belt between the connecting shaft and the rotary gear shaft rear end
Power connection, then when the movable block moves forward, the blade is to the chip block sections, the clamping device of rising progressively at this time
Work, the movable block moves backward when engage with the outer runner, and the outer runner can drive the clamping device turn of rising progressively
It starts building to make, so that gripping block drives the chip wax stone to rise, preparation is sliced next time.
Further technical solution, the power connector include the slot of stirring communicated with the conveyor chamber, and described group
Rotation is equipped with poke rod in dynamic slot, described to stir rod rear end and described stir is installed with rotation spring between slot, described to stir slot
Interior sliding is equipped with link block, and the link block left end and described stir are installed with compression spring between slot, described to stir slot left end
Wall is fixedly installed with magnet block, the link block and the magnet block can magnetic connect, it is described to stir the rotation of slot left side and be equipped with
Drive splines set, the drive splines set left end spline are connected with splined sleeve, lead between the splined sleeve and the mandrel
It crosses synchronous toothed belt to be connected by power, splined shaft, the splined shaft and the non-spline of the splined sleeve is equipped in the splined sleeve
Connection, when drive splines set is connect with the splined shaft spline, then the rotary state of the splined sleeve can drive institute
Splined shaft rotation is stated, described to stir the extruding slot for being equipped on front side of slot and communicating with the conveyor chamber, sliding is equipped in the extruding slot
Crushing block is installed with extruding spring, is equipped with link slot on front side of the extruding slot between the crushing block left end and the extruding slot,
The extruding slot with it is described between be connected to be equipped with and push away slot, it is described to push away sliding in slot and be equipped with pushing block, be equipped in the pushing block and abut slot,
The abutting slot is abutted with the crushing block, and the interior sliding is equipped with connecting rod, and the connecting rod right end and the pushing block are connected,
The connection rod rear end is installed with adjustable spring, and the connecting rod left end is installed with resetting block, and institute is protruded into the resetting block rear end
It states and stirs in slot and the resetting block rear end can be abutted with the link block, then the movable block promotion crushing block is to moving to left
When dynamic, so that the resetting block moves back, the link block is separated with the magnet block, then the drive splines set and the flower
Key axis is detached from spline connection.
Further technical solution, the lifting device include the lifting chamber communicated with the cold water cavity, the lifting chamber
Interior rotation is equipped with elevating screw, and elevator is threaded on the elevating screw, and the elevator right end and the water tank are solid
Even, be equipped with rotation chamber on the upside of the lifting chamber, the intracavitary rotation of rotation is equipped with the rotation axis extended downwardly, the rotation axis and
It is connected by power between the elevating screw by connecting belt, is installed with torsional spring in the rotation axis, it is intracavitary to be located at the rotation
Rotation axis upper end be installed with the 7th bevel gear, the 7th bevel gear right end engagement is connected with the 8th bevel gear, the spline
Axis left end and the 8th bevel gear are connected, then the elevating screw can be driven to rotate when the splined shaft rotates, to control
The lifting of the water tank.
The beneficial effects of the present invention are: the present invention is sliced chip wax stone by the movement of blade, so that chip wax
Integrity degree in block slicing processes is high, the qualification rate of organization chip obtained is improved, secondly, organization chip obtained is directly placed
It is unfolded in cold water, continuity operates the structural stability that organization chip can be improved.
Detailed description of the invention
Fig. 1 is a kind of organization chip of observation of cell signal conduction path of the invention and the inside entirety of cell line chip
Structural schematic diagram；
Fig. 2 is " A-A " direction structure schematic diagram in Fig. 1 in the present invention；
Fig. 3 is " B-B " direction structure schematic diagram in Fig. 2 in the present invention；
Fig. 4 is " C-C " direction structure schematic diagram in Fig. 3 in the present invention；
Fig. 5 is " D-D " direction structure schematic diagram in Fig. 2 in the present invention；
Fig. 6 is " E-E " direction structure schematic diagram in Fig. 5 in the present invention；
Fig. 7 is " F " enlarged structure schematic diagram in Fig. 2 in the present invention；
Fig. 8 is " G " enlarged structure schematic diagram in Fig. 7 in the present invention.
Specific embodiment
Below with reference to Fig. 1-8, the present invention is described in detail, for sake of convenience, now such as to hereafter described orientation regulation
Under: hereafter described up and down direction is consistent with the up and down direction of Fig. 1 projection relation itself.
Referring to Fig.1-8, the organization chip of a kind of observation of cell signal conduction path of embodiment according to the present invention and thin
Born of the same parents system chip, including slice body 10, the slice body 10 is interior to be equipped with biopsy cavity 11, is equipped with folder of rising progressively in the biopsy cavity 11
Tight device 601 is placed with chip wax stone 13 on the clamping device 601 of rising progressively, and the 601 front and back side of clamping device of rising progressively is symmetrical
Be equipped with baffle 18,18 lower end of baffle is fixedly arranged in the biopsy cavity 11, and rotation is equipped on rear side of the clamping device 601 of rising progressively
Shaft 28, the rotary shaft 28 or so are installed with fixed link 29, and blade 30 is fixedly installed between the fixed link 29, described to cut
Conveying device 602 is equipped on the left of piece chamber 11,28 left end of rotary shaft is connected by power with the conveying device 602, the conveying
Device 602 drives the back-and-forth motion of rotary shaft 28 to be sliced the chip wax stone 13, sets on the downside of the conveying device 602
Dynamic transfer device 603, the power transmission 603 and the conveying device 602 and the clamping device 601 of rising progressively are dynamic
Power connects, and power connector 604 is equipped on front side of the conveying device 602, is equipped with lifting on the downside of the power connector 604
Device 605 is equipped with cold water cavity 45 on front side of the biopsy cavity 11, is equipped with water tank 46, the water tank 46 and institute in the cold water cavity 45
It states lifting device 605 to be connected by power, cavity 47 is equipped in the water tank 46, the 45 right end wall of cold water cavity is equipped with the water pipe of circulation
50, the water pipe 50 is interior to be equipped with water supply pump 49, when the conveying device 602 triggers the power connector 604 work, then
The lifting device 605 can drive the water tank 46 to go up and down, and the chip wax stone 13 after the slice on the blade 30 is put
It is placed in the cavity 47, flows the water in the cold water cavity 45 from right to left, then
The chip wax stone 13 flows to 45 inner cold water of cold water cavity drift and opens, and with subsequent operation.
Valuably or illustratively, the chip wax stone 13 is made of numerous tissue core proper alignments, wherein tissue core
It is to acquire to get from the donor tissue wax stone with case research, the acceptor wax block of blank is equipped with numerous and tissue core number
Corresponding hole is measured, the tissue core of extraction is placed in the hole of acceptor wax block, to form the chip wax stone 13, the chip
Wax stone 13 forms organization chip after sectioning.
Valuably or illustratively, the clamping device 601 of rising progressively includes gripping block 14, is equipped with folder in the gripping block 14
Tight slot 16, the interior rotation of the clamping slot 16 are equipped with two-way lead screw 19, are threaded with gripping block 17, institute on the two-way lead screw 19
Chip wax stone 13 is stated between the gripping block 17, is communicated with the outside world on the left of the biopsy cavity 11 and is equipped with connection chamber 21, it is described
It is connected to sliding in chamber 21 and is equipped with connection block 20, two-way 19 left end of lead screw is through the connection block 20 and stretches to the external world, institute
Two-way lead screw 19 is stated with the block 20 that is connected to by bearing rotation connection, two-way 19 left end of lead screw is installed with adjusting block 22,
It is communicated in the 11 right end wall of biopsy cavity and is equipped with slot 15 of rising progressively, rotation is equipped with threaded shaft 12, the folder in the slot 15 of rising progressively
Tight 14 right end of block is threadedly engaged with the threaded shaft 12 to be connect, and is communicated on the upside of the slot 15 of rising progressively and is equipped with engagement chamber 23, described
Protrude into the engagement chamber 23 and be installed with first bevel gear 26,26 right end of the first bevel gear engagement in 12 upper end of threaded shaft
It is connected with and resets bevel gear 91,91 right side of reset bevel gear, which is installed with, resets shaft 92,92 right end of reset shaft
It protrudes into the external world and is installed with and reset switch block 93,26 rear end of first bevel gear engagement is connected with second bevel gear 24, and described the
Two bevel gears 24 do not engage with the reset bevel gear 91, are installed with rotary gear shaft 25, institute at 24 axle center of second bevel gear
25 rear end of rotary gear shaft is stated to be connected by power with the power transmission 603；The clamping is adjusted in the rotation of adjusting block 22
The distance between block 17, to clamp the chip wax stone 13, the chip wax stone 13 by and slice it is primary after, the gripping block
14, which rise the drive chip wax stone 13, rises certain distance, this distance is equal to the thickness of required organization chip.
Valuably or illustratively, the conveying device 602 includes conveyor chamber 27, and rotation, which is equipped with, in the conveyor chamber 27 turns
Screw rod 32 is moved, movable block 31 is threaded on the rotary screw rod 32, is equipped with swivelling chute 33, the rotation in the movable block 31
28 left end of shaft extends in the swivelling chute 33 and the rotary shaft 28 is rotatablely connected with the 33 left end wall of swivelling chute, in institute
It states and is installed with rotating wheel 34 in the rotary shaft 28 in swivelling chute 33,34 upper end of rotating wheel is installed with shifting block 35, described
Reset spring 90 is installed between 34 lower end of rotating wheel and the conveyor chamber 27,27 upper end wall of conveyor chamber is fixed, and there are four solid
Determine block 36, the movable block 31 drives the rotary shaft 28 to move forward, can be by the blade 30 by the chip wax stone 13
Slice, with the continued advancement of the movable block 31, under the mating reaction of the fixed block 36 and the shifting block 35, so that institute
It states rotating wheel 34 and drives 30 reciprocally swinging of blade, then the organization chip on the blade 30 is fallen into the cavity 47, institute
It states and is communicated on front side of conveyor chamber 27 equipped with drive cavity 69,69 front bulkhead of drive cavity is installed with engine 71, the rotary screw rod
32 front ends are connected by power in the engine 71, are installed with triconodont on the rotary screw rod 32 in the drive cavity 69
Wheel 70,70 left end of the third hand tap gear engagement are connected with the 4th bevel gear 72, and 72 left side of the 4th bevel gear is installed with
Mandrel 73,73 left end of mandrel are connected by power with the power connector 604.
Valuably or illustratively, the power transmission 603 include communicated with the conveyor chamber 27 unidirectionally turn chamber
37, described unidirectionally to turn to be rotated in chamber 37 equipped with outer runner 38, the outer runner 38 engages connection with 31 lower end surface of movable block,
One way engagement is connected with handle ratchet wheel 39 in the outer runner 38, described unidirectionally to turn to communicate on the left of chamber 37 equipped with connecting cavity 40, described
Rotation is equipped with the 5th bevel gear 41 in connecting cavity 40, and the 5th bevel gear 41 is connected with the handle ratchet wheel 39, the 5th cone
The engagement of 41 rear end of gear is connected with the 6th bevel gear 42, and connecting shaft 43, the company are installed at 42 axle center of the 6th bevel gear
It is connected by power between 25 rear end of spindle 43 and the rotary gear shaft by driving belt 44, then the movable block 31 moves forward
When, the blade 30 is sliced the chip wax stone 13, the work of clamping device 601 of rising progressively described at this time, the movable block 31 to
When movement is engaged with the outer runner 38 afterwards, the outer runner 38 can drive 601 rotation work of clamping device of rising progressively, so that
Gripping block 14 drives the chip wax stone 13 to rise, and preparation is sliced next time.
Valuably or illustratively, the power connector 604 includes stirring slot 65 with what the conveyor chamber 27 communicated,
Described stir in slot 65 rotates equipped with poke rod 66, and 66 rear end of poke rod and described stir are installed with rotation bullet between slot 65
Spring 67, described stir in slot 65 are slided equipped with link block 64, and 64 left end of link block and described stir are installed between slot 65
Compression spring 68, the 65 left end wall of slot of stirring are fixedly installed with magnet block 62, and the link block 64 can with the magnet block 62
Magnetic connection, described to stir the rotation of 65 left side of slot equipped with drive splines set 63, the drive splines covers the connection of 63 left end splines
There is splined sleeve 60, is connected by power between the splined sleeve 60 and the mandrel 73 by synchronous toothed belt 61, the spline
Splined shaft 59 is equipped in sleeve 60, the splined shaft 59 is connect with the non-spline of the splined sleeve 60, when the drive splines set
63 with 59 spline of splined shaft when connecting, then the rotary state of the splined sleeve 60 can drive the splined shaft 59 to rotate,
Described to stir the extruding slot 82 for being equipped on front side of slot 65 and communicating with the conveyor chamber 27, sliding is equipped with crushing block in the extruding slot 82
81, it is installed between 81 left end of crushing block and the extruding slot 82 and squeezes spring 83, the company of being equipped on front side of the extruding slot 82
Access slot 76, is connected between the extruding slot 82 and described 76 to be equipped with and pushes away slot 80, described to push away sliding in slot 80 and be equipped with pushing block 79, described
It being equipped in pushing block 79 and abuts slot 84, the abutting slot 84 is abutted with the crushing block 81, and sliding is equipped with connecting rod 78 in described 76,
78 right end of connecting rod and the pushing block 79 are connected, and 78 rear end of connecting rod is installed with adjustable spring 77, the connecting rod
78 left ends are installed with resetting block 75, and described stir in slot 65 and 75 rear end of resetting block can be with is protruded into 75 rear end of resetting block
The link block 64 abuts, then when the movable block 31 pushes the crushing block 81 to be moved to the left, so that after the resetting block 75
It moves, the link block 64 is separated with the magnet block 62, then the drive splines set 63 is detached from spline with the splined shaft 59
Connection.
Valuably or illustratively, the lifting device 605 includes the lifting chamber 52 communicated with the cold water cavity 45, described
It goes up and down rotation in chamber 52 and is equipped with elevating screw 48, be threaded with elevator 51, the elevator 51 on the elevating screw 48
Right end and the water tank 46 are connected, and rotation chamber 56 are equipped on the upside of the lifting chamber 52, rotation is equipped with downward in the rotation chamber 56
The rotation axis 54 of extension is connected by power between the rotation axis 54 and the elevating screw 48 by connecting belt 53, described turn
Torsional spring 55 is installed on moving axis 54,54 upper end of rotation axis in the rotation chamber 56 is installed with the 7th bevel gear 58, described
The engagement of 7th bevel gear, 58 right end is connected with the 8th bevel gear 57, and 59 left end of splined shaft and the 8th bevel gear 57 are solid
Even, then the elevating screw 48 can be driven to rotate when the splined shaft 59 rotates, to control the lifting of the water tank 46.
When original state, movable block 31 is located at right limit position in conveyor chamber 27, then blade 30 is located at 18 rear side of baffle
The chip wax stone 13 of slice is placed in gripping block 14 by position, and rotation adjusting block 22 moves towards gripping block 17, from
And clamp chip wax stone 13, rotation manually resets switch block 93, so that threaded shaft 12 rotates, then gripping block 14 is gone up and down, and adjusts
The height of chip wax stone 13 prepares slice work.
When slice, 71 work belt of engine is moved rotary screw rod 32 and is rotated, then movable block 31 moves forward, before band cutting blade 30
It moves so that blade 30 is sliced chip wax stone 13, the organization chip formed after slice is located on blade 30,31 band of movable block
When cutting blade 30 is moved to 45 upside of cold water cavity, movable block 31 pushes poke rod 66 to rotate, so that link block 64 moves to left and magnet
The connection of 62 magnetic of block to be moved piece 31 and when the disengaging of poke rod 66, is stirred at this point, drive splines set 63 engage with splined shaft 59
Bar 66 restores initial position, and link block 64 is connect with magnet block 62 always at this time, then engine 71 can drive splined shaft 59 to rotate,
So that elevating screw 48 rotates, then elevator 51 drives water tank 46 to rise, and blade 30 is located in cavity 47 at this time, with movable block
31 lasting forward movements, under the effect of fixed block 36, so that rotating wheel 34 is swung, to swing with cutting blade 30, so that blade
Organization chip on 30 flows in cavity 47, then movable block 31, which moves forward to, pushes crushing block 81 when being moved to the left, pushing block 79 to
After move link block 64 is separated with magnet block 62 so that resetting block 75 moves back, then link block 64 restore initial position, pass at this time
Dynamic spline housing 63 is detached from splined shaft 59, under the effect of torsional spring 55 rotation axis 54 is rotated backward, then elevating screw 48 is reversed
Rotation, so that water tank 46 drives the organization chip in it to drop in cold water cavity 45, water supply pump 49 works meeting so that cold water cavity 45
Interior cold water flows from right to left, then the organization chip in cavity 47 flows in cold water cavity 45 and the smooth expansion of organization chip,
Convenient for subsequent observation use；
Engine 71 rotates backward work, so that movable block 31 moves backward, then crushing block 81 moves to right, and drives resetting block 75 backward
It is mobile to restore initial position, when movable block 31, which is moved rearwardly into, to be engaged with outer runner 38, as movable block 31 moves back so that outer
Runner 38 rotates, then handle ratchet wheel 39 rotates, and drives threaded shaft 12 to rotate by driving belt 44, then gripping block 14 drives chip
Wax stone 13 moves up a Duan Gaodu, and when engine 71 rotates forward, movable block 31 drives outer runner 38 to rotate, but handle ratchet wheel 39
It does not rotate, with the forward movement again of movable block 31, completes to be made next time to the slice work of chip wax stone 13 with this
Organization chip be unfolded naturally in cold water cavity 45.
The beneficial effects of the present invention are: the present invention is sliced chip wax stone by the movement of blade, so that chip wax
Integrity degree in block slicing processes is high, the qualification rate of organization chip obtained is improved, secondly, organization chip obtained is directly placed
It is unfolded in cold water, continuity operates the structural stability that organization chip can be improved.
Those skilled in the art can define, can in the case of not departing from overall spirit of the invention and design
To make the various modifications for above embodiments.It falls within the scope of protection of the present invention.Protection scheme of the invention
It is subject to claims appended hereto.
Claims (7)
1. a kind of organization chip and cell line chip of observation of cell signal conduction path, including slice body, the slicer
It is equipped with biopsy cavity in vivo, is equipped with clamping device of rising progressively in the biopsy cavity, is placed with chip wax stone on the clamping device of rising progressively,
Side is symmetrically equipped with baffle before and after the clamping device of rising progressively, and the baffle lower end is fixedly arranged in the biopsy cavity, described to rise progressively
Rotary shaft is equipped on rear side of clamping device, described rotary shaft or so is installed with fixed link, is fixedly installed with knife between the fixed link
Piece, the biopsy cavity left side are equipped with conveying device, and the rotary shaft left end is connected by power with the conveying device, the conveying dress
It sets and the rotary shaft back-and-forth motion is driven to be sliced the chip wax stone, power transmitting dress is equipped on the downside of the conveying device
It sets, the power transmission is connected by power with the conveying device and the clamping device of rising progressively, on front side of the conveying device
Equipped with power connector, it is equipped with lifting device on the downside of the power connector, is equipped with cold water cavity, institute on front side of the biopsy cavity
It states and is equipped with water tank in cold water cavity, the water tank is connected by power with the lifting device, and cavity, the cold water are equipped in the water tank
Chamber right end wall is equipped with the water pipe of circulation, and water supply pump is equipped in the water pipe, is connected by power dress described in the conveying device triggering
When setting work, then the lifting device can drive the water tank to go up and down, by the chip wax stone after the slice on the blade
It places in the cavity, flows the water in the cold water cavity from right to left, then it is described
Chip wax stone flows to cold water cavity inner cold water drift and opens, and with subsequent operation.
2. the organization chip and cell line chip of a kind of observation of cell signal conduction path according to claim 1, special
Sign is: the chip wax stone is made of numerous tissue core proper alignments, wherein tissue core is from case research
Acquisition is got in donor tissue wax stone, and the acceptor wax block of blank is equipped with numerous holes corresponding with tissue core quantity, by extraction
Tissue core is placed in the hole of acceptor wax block, so that the chip wax stone is formed, chip wax stone shape after sectioning
At organization chip.
3. the organization chip and cell line chip of a kind of observation of cell signal conduction path according to claim 1, special
Sign is: the clamping device of rising progressively includes gripping block, and clamping slot is equipped in the gripping block, and rotation is equipped in the clamping slot
Two-way lead screw is threaded with gripping block on the two-way lead screw, and the chip wax stone is described to cut between the gripping block
It is communicated with the outside world on the left of piece chamber and is equipped with connection chamber, the intracavitary sliding of connection is equipped with connection block, and the two-way lead screw left end is run through
The connection block and stretch to the external world, the two-way lead screw with it is described be connected to block and pass through bearing be rotatablely connected, the two-way lead screw
Left end is installed with adjusting block, communicates in the biopsy cavity right end wall and is equipped with slot of rising progressively, and rotation turns equipped with screw thread in the slot of rising progressively
Axis, the gripping block right end is threadedly engaged with the threaded shaft to be connect, and is communicated on the upside of the slot of rising progressively and is equipped with engagement chamber, described
Threaded shaft upper end protrudes into that the engagement is intracavitary and be installed with first bevel gear, and the first bevel gear right end engagement is connected with multiple
Position bevel gear, the reset bevel gear right side are installed with reset shaft, and the reset shaft right end protrudes into the external world and is installed with
Switch block is resetted, the first bevel gear rear end engagement is connected with second bevel gear, and tooth is bored in the second bevel gear and the reset
Wheel does not engage, and rotary gear shaft is installed at the second bevel gear axle center, and the rotary gear shaft rear end and power transmitting fill
It sets and is connected by power；The distance between described gripping block is adjusted in the adjusting block rotation, described to clamp the chip wax stone
Chip wax stone by and slice it is primary after, the gripping block, which rises, drives the chip wax stone to rise certain distance, this distance is equal to
The thickness of required organization chip.
4. the organization chip and cell line chip of a kind of observation of cell signal conduction path according to claim 1, special
Sign is: the conveying device includes conveyor chamber, and rotation is equipped with rotary screw rod, screw thread on the rotary screw rod in the conveyor chamber
It is connected with movable block, swivelling chute is equipped in the movable block, the rotary shaft left end extends in the swivelling chute and the rotation
Shaft and swivelling chute left end wall are rotatablely connected, and are installed with rotating wheel in the rotary shaft in the swivelling chute, described
Rotating wheel upper end is installed with shifting block, and reset spring, the conveyor chamber are installed between the rotating wheel lower end and the conveyor chamber
Upper end wall is fixed there are four fixed block, and the movable block drives the rotary shaft to move forward, can will be described by the blade
Chip block sections, with the continued advancement of the movable block, under the mating reaction of the fixed block and the shifting block, so that
The rotating wheel drives the blade reciprocation to swing, then the organization chip on the blade is fallen into the cavity, the conveying
Communicate equipped with drive cavity on front side of chamber, the drive cavity front bulkhead is installed with engine, the rotary screw rod front end be connected by power in
The engine is installed with third hand tap gear, the third hand tap gear left end on the rotary screw rod in the drive cavity
Engagement is connected with the 4th bevel gear, and the 4th bevel gear left side is installed with mandrel, and the mandrel left end and the power connect
Connection device is connected by power.
5. the organization chip and cell line chip of a kind of observation of cell signal conduction path according to claim 1, special
Sign is: the power transmission include communicated with the conveyor chamber unidirectionally turn chamber, it is described unidirectionally to turn intracavitary rotation and be equipped with
Outer runner, the outer runner engage connection with the movable block lower end surface, and one way engagement is connected with handle ratchet wheel in the outer runner,
Described unidirectionally to turn to communicate on the left of chamber equipped with connecting cavity, rotation is equipped with the 5th bevel gear, the 5th bevel gear in the connecting cavity
It is connected with the handle ratchet wheel, the 5th bevel gear rear end, which is engaged, is connected with the 6th bevel gear, at the 6th bevel gear axle center
It is installed with connecting shaft, is connected by power between the connecting shaft and the rotary gear shaft rear end by driving belt, then the movement
When block moves forward, the blade is to the chip block sections, the clamping device work of rising progressively described at this time, the movable block to
When movement is engaged with the outer runner afterwards, the outer runner can drive the clamping device rotation work of rising progressively, so that gripping block
The chip wax stone is driven to rise, preparation is sliced next time.
6. the organization chip and cell line chip of a kind of observation of cell signal conduction path according to claim 1, special
Sign is: the power connector includes the slot of stirring communicated with the conveyor chamber, it is described stir rotation in slot and be equipped with stir
Bar, it is described to stir rod rear end and described stir is installed with rotation spring between slot, it is described to stir sliding in slot and be equipped with link block, institute
It states link block left end and described stir is installed with compression spring between slot, the slot left end wall of stirring is fixedly installed with magnet block,
The link block and the magnet block can magnetic connect, it is described to stir the rotation of slot left side and be equipped with drive splines set, the transmission
Spline housing left end spline is connected with splined sleeve, is connected between the splined sleeve and the mandrel by synchronous tooth profile drive
It connects, splined shaft is equipped in the splined sleeve, the splined shaft is connect with the non-spline of the splined sleeve, when the drive splines
When set is connect with the splined shaft spline, then the rotary state of the splined sleeve can drive the splined shaft to rotate, and described group
The extruding slot communicated with the conveyor chamber is equipped on front side of dynamic slot, sliding is equipped with crushing block in the extruding slot, and the crushing block is left
Be installed with extruding spring between end and the extrudings slot, equipped with link slot on front side of the extrudings slot, the extruding slot with it is described it
Between connection be equipped with and push away slot, it is described to push away sliding in slot and be equipped with pushing block, be equipped in the pushing block and abut slot, the abuttings slot and described crowded
Briquetting abuts, and the interior sliding is equipped with connecting rod, and the connecting rod right end and the pushing block are connected, and the connection rod rear end is fixed
Have an adjustable spring, the connecting rod left end is installed with resetting block, the resetting block rear end protrude into it is described stir it is in slot and described multiple
Position block rear end can be abutted with the link block, then when the movable block pushes the crushing block to be moved to the left, so that the reset
Block moves back, and the link block is separated with the magnet block, then the drive splines set is detached from spline with the splined shaft and connect.
7. the organization chip and cell line chip of a kind of observation of cell signal conduction path according to claim 1, special
Sign is: the lifting device includes the lifting chamber communicated with the cold water cavity, and the intracavitary rotation of lifting is equipped with elevating screw,
Elevator is threaded on the elevating screw, the elevator right end and the water tank are connected, set on the upside of the lifting chamber
There is rotation chamber, the intracavitary rotation of rotation is equipped with the rotation axis extended downwardly, leads between the rotation axis and the elevating screw
It crosses connection belt to be connected by power, is installed with torsional spring in the rotation axis, the rotation axis upper end intracavitary positioned at the rotation is installed with
7th bevel gear, the 7th bevel gear right end engagement are connected with the 8th bevel gear, the splined shaft left end and the 8th cone
Gear is connected, then the elevating screw can be driven to rotate when the splined shaft rotates, to control the lifting of the water tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910661637.8A CN110346193A (en) | 2019-07-22 | 2019-07-22 | A kind of organization chip and cell line chip of observation of cell signal conduction path |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910661637.8A CN110346193A (en) | 2019-07-22 | 2019-07-22 | A kind of organization chip and cell line chip of observation of cell signal conduction path |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110346193A true CN110346193A (en) | 2019-10-18 |
Family
ID=68179656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910661637.8A Withdrawn CN110346193A (en) | 2019-07-22 | 2019-07-22 | A kind of organization chip and cell line chip of observation of cell signal conduction path |
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| Country | Link |
|---|---|
| CN (1) | CN110346193A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112763342A (en) * | 2020-12-30 | 2021-05-07 | 中航试金石检测科技(大厂)有限公司 | Composite material high-temperature compression tool and tensile testing machine |
-
2019
- 2019-07-22 CN CN201910661637.8A patent/CN110346193A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112763342A (en) * | 2020-12-30 | 2021-05-07 | 中航试金石检测科技(大厂)有限公司 | Composite material high-temperature compression tool and tensile testing machine |
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Application publication date: 20191018 |
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