CN110339259B

CN110339259B - Medicine for removing mites and preparation method thereof

Info

Publication number: CN110339259B
Application number: CN201910646975.4A
Authority: CN
Inventors: 何彬生
Original assignee: Changsha Medical University
Current assignee: Changsha Medical University
Priority date: 2019-07-17
Filing date: 2019-07-17
Publication date: 2021-08-27
Anticipated expiration: 2039-07-17
Also published as: CN110339259A

Abstract

The invention discloses a medicament for removing mites and a preparation method thereof, wherein the medicament comprises the following components: ethanol, honeysuckle flower extract, phellodendron amurense extract, essence and pigment, wherein 0.5-0.8g of honeysuckle flower extract, 0.5-0.6g of phellodendron amurense extract, 2g of essence and 1g of pigment are dissolved in 1L of ethanol. The concentration of the ethanol is 35.5-39%. The preparation method of the medicine comprises the following steps: dissolving 0.5-0.8g of honeysuckle flower extract and 0.5-0.6g of phellodendron amurense extract in 130ml of 100-one organic solvent absolute ethyl alcohol, adding 2g of essence and 1g of pigment, then adding 260ml of 255-one organic solvent absolute ethyl alcohol, uniformly mixing, adding water to 1000ml, uniformly mixing, and filtering to obtain the extract. The medicine prepared by the invention solves the problems that metronidazole is an antibiotic which is not beneficial to long-term use, 10% of sulfur ointment has special smell, and patients are difficult to adhere to the sulfur ointment to influence the curative effect in the prior art, and the medicine removes mites, has good curative effect, no side effect and quick response, and has simple preparation method and high efficiency.

Description

Medicine for removing mites and preparation method thereof

Technical Field

The invention belongs to the field of skin science and technology, and relates to a medicine for removing mites and a preparation method thereof.

Background

Demodex, a permanent small parasitic mite, belongs to the order Eudermatales, Demodex family. The mite is mainly parasitized in hair follicle and sebaceous gland of human body, can cause the pathological changes of hair follicle expansion, epithelial deformation, sebaceous gland secretion obstruction and the like, and is also related to dermatosis such as acne rosacea, folliculitis, acne, seborrheic dermatitis and the like. The infection rate of domestic people to Demodex mites is rising continuously, and the facial Demodex mite infection has the most influence on the physical and mental health of people.

Currently, the main therapeutic agents for demodex are 2% metronidazole cream and 10% sulphur ointment. 2% metronidazole cream, treat folliculitis dermatitis with metronidazole cream, has avoided the adverse reaction that the oral medicament causes, the curative effect is precise, cheap, but metronidazole belongs to antibiotic and is unfavorable for long-term use; the 10% sulfur ointment has special odor, and patients are often hard to persist and the curative effect is affected.

Disclosure of Invention

In order to achieve the aim, the invention provides a medicament for removing mites and a preparation method thereof, which solve the problems that metronidazole existing in the prior art belongs to antibiotics and is not beneficial to long-term use, 10% of sulfur ointment has special smell, and patients are difficult to stick to the ointment to influence curative effect.

The invention also aims to provide a preparation method of the medicine.

In order to solve the technical problems, the invention adopts the technical scheme that the medicine for removing mites comprises the following components:

ethanol, honeysuckle flower extract, phellodendron amurense extract, essence and pigment, wherein 0.5-0.8g of honeysuckle flower extract, 0.5-0.6g of phellodendron amurense extract, 2g of essence and 1g of pigment are dissolved in 1L of ethanol.

Further, the concentration of the ethanol is 35.5-39%.

Further, the medicament for removing mites comprises the following components: ethanol, honeysuckle flower extract, phellodendron amurense extract, essence and pigment, wherein 0.8g of honeysuckle flower extract, 0.6g of phellodendron amurense extract, 2g of essence and 1g of pigment are dissolved in 1L of ethanol.

Further, the ethanol concentration is 38.5%.

The invention adopts another technical scheme that a preparation method of a medicament for removing mites comprises the following steps:

dissolving 0.5-0.8g of honeysuckle flower extract and 0.5-0.6g of phellodendron amurense extract in 130ml of 100-one organic solvent absolute ethyl alcohol, adding 2g of essence and 1g of pigment, then adding 260ml of 255-one organic solvent absolute ethyl alcohol, uniformly mixing, adding water to 1000ml, uniformly mixing, and filtering to obtain the extract.

Wherein, the volatile organic compounds of the lonicera macranthoides flower are determined by GC-MS, and 45 components are detected: wherein 10 kinds of hydrocarbon substances account for 44.03 percent of the total amount of volatile organic compounds, 8 kinds of acid compounds account for 24.14 percent, 9 kinds of alcohol compounds account for 6.34 percent, 9 kinds of ester compounds account for 8.452 percent, and 6 kinds of ketone substances account for 8.85 percent. The relative content of the components more than 1 percent is 22: wherein the content of palmitic acid is up to 12.34%, and the total content of 5 compounds such as n-nonadecane, n-nonacosane, heneicosane, pentadecane and n-dotriacontane is 35.10%. The volatile organic compounds of lonicera macranthoides flower have good anti-inflammatory and antibacterial effects. Phellodendron amurense extract: clearing heat and drying dampness, purging fire and removing steam, and removing toxic substance and treating sore, and can be used for treating damp-heat dysentery, pyocutaneous disease, eczema and pruritus, and has adjuvant effect in killing acarid. Honeysuckle flower, phellodendron amurense extract and ethanol are the effective components of the invention, and acaricidal experiments prove that the concentration set by the formula is the optimal concentration, and the concentration is ineffective when the concentration is too low, and skin discomfort or side effects of a human body can be generated when the concentration is too high. Essence: the product has certain fragrance and is easy to be accepted by consumers. Pigment: the product has certain color and luster, the appearance is satisfied by consumers, and the product is easy to accept. The preparation method comprises adding ethanol twice, dissolving various components for the first time, and diluting for the second time.

The invention has the beneficial effects that:

compared with the traditional medicine for treating demodex, the medicine for removing demodex prepared by the invention does not contain antibiotics, can be used for a long time, has no special bad smell (the medicine has pleasant aromatic smell and is derived from natural extracts and essences contained in the medicine), does not influence the use of a patient, and has good curative effect, no side effect, quick response, simple preparation method and high efficiency. The medicament of the invention not only can be used for killing demodex on human skin, has better effect than metronidazole, but also can kill dust mites more effectively and rapidly than similar products in the market, can be used for articles such as mattresses, pillows, bedding and the like, and has wide application; meanwhile, the product has volatility, no residue and trace after the product is acted, and good patient compliance.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a diagram of a sample screened for mite infested persons.

FIG. 2 is a microscopic (under 10-fold microscope) view of Demodex mites.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Dissolving 0.5g flos Lonicerae extract and 0.5g cortex Phellodendri extract in 100ml anhydrous ethanol, adding 2g essence and 1g pigment, adding 255ml anhydrous ethanol, mixing, adding water to 1000ml, mixing, and filtering.

Example 2

Dissolving 0.8g flos Lonicerae extract and 0.6g cortex Phellodendri extract in 120ml anhydrous ethanol, adding 2g essence, 1g pigment, and 265ml anhydrous ethanol, mixing, adding water to 1000ml, mixing, and filtering.

Example 3

Dissolving 0.7g flos Lonicerae extract and 0.5g cortex Phellodendri extract in 130ml anhydrous ethanol, adding 2g essence, 1g pigment, adding 260ml anhydrous ethanol, mixing, adding water to 1000ml, mixing, and filtering.

Example 4

Dissolving 0.9g flos Lonicerae extract and 0.8g cortex Phellodendri extract in 300ml anhydrous ethanol, adding 2g essence and 1g pigment, adding 450ml anhydrous ethanol, mixing, adding water to 1000ml, mixing, and filtering.

Example 5

Dissolving 0.4g flos Lonicerae extract and 0.4g cortex Phellodendri extract in 100ml anhydrous ethanol, adding 2g essence, 1g pigment, adding 150ml anhydrous ethanol, mixing, adding water to 1000ml, mixing, and filtering.

For the drugs for removing mites prepared in 5 groups of examples, the drug prepared in example 2 had the best efficacy, and the drugs prepared in examples 1 and 3 had the next best efficacy, the drug prepared in example 4 had an ethanol concentration of 75%, and the concentration thereof was too high, which irritated the skin of the human body, and thus it was not suitable for use, and the drug prepared in example 5 had an ethanol concentration of 25%, and the concentration thereof was too low, which resulted in poor mite-killing effect, and thus it was not suitable for use; experiments were conducted in the following examples using the drug prepared in example 2 and the prior art drug for mite removal in effect verification. 70% -75% ethanol concentration is high, so that protein coagulation denaturation effect is good, but irritation to skin is high correspondingly.

Example Effect verification

1. Preparation of Experimental materials

Experimental animals: demodex mites and dust mites

Experimental group 1.: pharmaceutical composition, Using the drugs prepared in example 2

Experimental group 2: metronidazole control group, 2% Metronidazole, dissolved in 38.5% ethanol

Experimental group 3: ethanol control group, using 38.5% ethanol

Experimental group 4: saline control group, saline

Experimental group 5: the control group was good at fortune Henry.

2. Experimental methods

(1) Screening of Demodex infested persons

The primary screening adopts a transparent adhesive tape method. The demodex infected person is required to stick transparent adhesive tapes on the two sides of the nose and the nasal alar before sleeping at night, take down the transparent adhesive tapes on a glass slide next morning and send the glass slide to a laboratory for microscopic examination.

The fine screen adopts an extrusion method. Selecting a person infected with the primarily screened demodex, scraping skin on two sides of the alar nose in a laboratory by using an acne compressor, placing the scraped object on a glass slide, adding a drop of normal saline, spreading, and performing microscopic examination.

(2) Demodex mite killing experiment

Select the fine-screen positive demodex infected persons, and use the squeezing method to take sebaceous gland secretions on double-concave glass slides in the laboratory as shown in fig. 1. Live mites (motile) were selected under a microscope, 80 microliters of the liquid medicine was added for treatment, after a cover glass was covered, a video was taken under a microscope at 4 × 10 times or manually observed, as shown in fig. 2, the worm stops moving and is recorded as dead, the dead time (accurate to min) is recorded, and the observation is stopped after 270min when the worm does not die.

(3) Mite killing experiment for dust mite

Firstly, dust mite samples are taken from bedding and beddings of student bedrooms and textile fibers absorbed by a dust collector.

Secondly, placing the textile fiber into a plate, observing under a microscope with 4 multiplied by 10 times, and determining whether live dust mites exist.

Thirdly, placing the live dust mites on a concave glass slide, adding 80 microliters of liquid medicine for treatment, covering a cover glass, and observing under a microscope 4 multiplied by 10 times.

Judging the death of dust mites: and continuously observing the dust mites for 30s every minute, wherein the dust mites do not move, changing to another classmate observation of the same group, and continuously observing the dust mites for two times by the two classmates to judge that the dust mites do not move and the dust mites die (the death time record is accurate to min).

3. Statistical analysis

Statistical analysis of the data obtained was performed using SPSS 17.0 software.

The death time of each group is tested by ANOVA, and the P <0.05 shows significant difference. And comparing every two after the fact by adopting an SNK (selective non-binding kinase) test method, wherein the difference of significance is that P is less than 0.05.

4. Screening results of mite infected persons

A total of 1600 20-year-old populations (students in school) are screened, 56 positive mites are detected after primary screening, and fine-screening live-insect examination and drug experiments are carried out.

TABLE 1 Demodex mite mortality time raw data

Grouping	Sample size (human)	Death time min (x + -s)
			Drug group	20	34.5±12.0
38.5% ethanol group	21	22.0±11.0
			Metronidazole group	22	61.5±20.0
Happy lucky Heng group	22	133.2±37.4
			Physiological saline group	20	140.4±23.6

TABLE 2 Demodex mite mortality time ANOVA test

	Sum of squares	df	Mean square	F	Significance of
						Between groups	254640.954	4	63660.238	118.824	0.000
In group	53575.180	100	535.752
						Total number of	308216.133	104

TABLE 3 Demodex mite mortality time

The group mean in the homogeneous subset will be displayed.

a. The harmonic mean sample size will be used 20.962.

b. The group sizes are not equal. The group-sized harmonic mean will be used. Class I error levels will not be guaranteed.

Table 4 raw data of death time of dust mites

TABLE 5 dust mite death time ANOVA test

	Sum of squares	df	Mean square	F	Significance of
						Between groups	82601.340	4	20650.335	20.683	0.000
In group	94847.900	95	998.399
						Total number of	177449.240	99

TABLE 6 dust mite mortality time

The group mean in the homogeneous subset will be displayed.

a. The harmonic mean sample size will be used-20.000.

5. Conclusion of the experiment

(1) The effect of the medicine of the invention on demodex is obviously different from that of metronidazole, normal saline and Happy Henry.

(2) The effect of the medicine of the invention on dust mites is obviously different from that of a metronidazole group, a normal saline group and a 38.5% ethanol group.

The medicament researched by the invention is effective to demodex, and has better effect than clinical metronidazole; it is also effective against dust mites, and has a similar but better effect than the clinical use of lucky henry. However, ethanol alone is effective on demodex and has poor effect on dust mites, but the ethanol alone is rarely used clinically for treating demodex infection. In clinical application, the medicine is applied to the skin surface of a demodex patient for 2-3 times every day, and is maintained for one week, and no toxic or side effect is found during the application of the medicine.

Claims

1. A medicament for killing mites, which is characterized by comprising the following components: dissolving 0.8g of lonicera macranthoides flower extract, 0.6g of phellodendron amurense extract, 2g of essence and 1g of pigment in 1L of ethanol;

the ethanol concentration is 38.5%.

2. A preparation method of a medicine for removing mites is characterized by comprising the following steps:

dissolving 0.8g Lonicera macranthoides flower extract and 0.6g cortex Phellodendri extract in 130ml anhydrous ethanol, adding 2g essence and 1g pigment, adding 255ml anhydrous ethanol, mixing, adding water to 1000ml, mixing, and filtering.