CN110317838A - Method for preparing ethanol by adopting cassava alcohol residues - Google Patents

Method for preparing ethanol by adopting cassava alcohol residues Download PDF

Info

Publication number
CN110317838A
CN110317838A CN201810265075.0A CN201810265075A CN110317838A CN 110317838 A CN110317838 A CN 110317838A CN 201810265075 A CN201810265075 A CN 201810265075A CN 110317838 A CN110317838 A CN 110317838A
Authority
CN
China
Prior art keywords
phase product
alcohol
solid phase
solid
hydrolytic process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201810265075.0A
Other languages
Chinese (zh)
Other versions
CN110317838B (en
Inventor
岳军
徐友海
胡世洋
宁艳春
屈海峰
王继艳
惠继星
金刚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Petrochina Co Ltd
Original Assignee
Petrochina Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Petrochina Co Ltd filed Critical Petrochina Co Ltd
Priority to CN201810265075.0A priority Critical patent/CN110317838B/en
Publication of CN110317838A publication Critical patent/CN110317838A/en
Application granted granted Critical
Publication of CN110317838B publication Critical patent/CN110317838B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention provides a method for preparing ethanol by adopting cassava alcohol residues. The method comprises the following steps: carrying out a first hydrolysis process on the cassava alcohol residues under the catalysis of alkali to obtain a first solid-phase product; under the catalysis of acid, carrying out a second hydrolysis process on the first solid-phase product to obtain a second solid-phase product; and carrying out saccharification and fermentation on the second solid-phase product to obtain the ethanol. Under the action of alkali, the cassava alcohol residues can be hydrolyzed by the first hydrolysis process, so that lignin is dissolved in water, and meanwhile, some phenolic substances can be generated, and the adsorption of the phenolic substances on the first solid-phase product can inhibit the subsequent alcohol fermentation process. In the second hydrolysis process, under the action of acid, the phenolic substance reacts with the acid and then is removed from the first solid-phase product, so as to obtain a second solid-phase product. The second hydrolysis process can remove the above phenolic substances inhibiting the fermentation process, and thus the second hydrolysis process is beneficial to improving the yield of ethanol in the fermentation process.

Description

The method that ethyl alcohol is prepared using cassava alcohol slag
Technical field
The present invention relates to biological chemical fields, in particular to a kind of method for preparing ethyl alcohol using cassava alcohol slag.
Background technique
China since Car-Use Ethanol Gasoline Promotion, has successively been established, using corn wheat as the Ji of raw material since 2001 Woods alcohol fuel, Henan day hat, Anhui rich Yuan Dengshuojia alcohol fuel company and cassava are China Oil and Food Import and Export Corporation's North Sea alcohol fuel of raw material Co., Ltd.Currently, striving grain with grain-production alcohol fuel and people, country, which has expressly provided, to forbid continuing developing grain alcohol item Mesh, and there are no relevant regulations in terms of cassava produces alcohol fuel, therefore are had become not using cassava production alcohol fuel Come the trend developed.However, how generated cassava alcohol slag handles, gives during using cassava production alcohol fuel Enterprise causes great burden.
According to the literature, 1 ton of Cassava-based ethanol of every production can about generate 0.33 ton of cassava alcohol slag, produce 300000 tons of cassava per year Alcohol fuel factory generates about 100,000 tons of cassava alcohol slag for 1 year.Currently, the processing mode of cassava alcohol slag, is mainly burned Or landfill, not still to the significant wastage of resource, while seriously polluting environment.
Contain higher cellulose and hemicellulose in cassava alcohol slag, if by preprocess method appropriate to cassiri Smart slag is pre-processed, and is converted ethyl alcohol for the cellulose in cassava alcohol slag, will be mitigated environmental pollution, is cassava alcohol slag It recycles and a practicable approach is provided.And existing there is a problem of that alcohol yied is low using cassava alcohol slag method.
Summary of the invention
It is existing to solve the main purpose of the present invention is to provide a kind of method for preparing ethyl alcohol using cassava alcohol slag There is a problem of that alcohol yied is low using the method that cassava alcohol slag prepares ethyl alcohol.
To achieve the goals above, a kind of method that ethyl alcohol is prepared using cassava alcohol slag is provided according to the present invention, side Method includes: that cassava alcohol slag is carried out the first hydrolytic process, obtains the first solid product under the catalytic action of alkali;In acid Under catalytic action, the first solid product is subjected to the second hydrolytic process, obtains second solid phase product;And by second solid phase product into Row diastatic fermentation, obtains ethyl alcohol.
Further, this method further includes adding in the first hydrolytic process to the reaction system of the first hydrolytic process The step of heat;Preferably, the temperature of the first hydrolytic process is 150~220 DEG C, and heating time is 1~2h.
Further, alkali is the mixed liquor of the hydrogen peroxide of 30wt% and the ammonium hydroxide of 5~15wt%, and the volume of hydrogen peroxide is ammonia The 10~20% of water volume.
Further, method further includes heating in the second hydrolytic process to the reaction system of the second hydrolytic process The step of;Preferably, the heating temperature of the second hydrolytic process is 160~210 DEG C, and heating time is 40~75min.
Further, acid is the aqueous sulfuric acid of 3~5wt% of concentration.
Further, before carrying out diastatic fermentation process, the above method further include: in being washed to second solid phase product Property after, then using ethanol water carry out alcohol wash processing;And ethyl alcohol is recycled by the way of flash distillation after alcohol washes processing, it obtains Second solid phase product.
Further, the volume fraction of the middle ethyl alcohol of ethanol water is 10~20%, and temperature is 80~100 DEG C, and alcohol is washed The time for the treatment of process is 30~60min.
Further, diastatic fermentation includes carrying out the mixture of second solid phase product, cellulase, inorganic salts and yeast The process of fermentation.
Further, the total amount relative to the reaction system of diastatic fermentation, the additional amount of cellulase are 15~25FPU/ g。
Further, inorganic salts are selected from urea and diammonium hydrogen phosphate;Preferably, the dosage of urea is 2~4g/L;It is preferred that Ground, the dosage of diammonium hydrogen phosphate are 1~2g/L.
Further, this method further includes the steps that supplementing the second hydrolysate during diastatic fermentation;Preferably, it mends The number for filling second solid phase product process is 2~4 times, and the time interval of the adjacent process of feed supplement twice is for 24 hours;Preferably, it mends After filling second solid phase product, the concentration of second solid phase product is 200~260g/L.
Further, the contents on dry basis of the lignin in second solid phase product be less than 10wt%, moisture content be 5~ 20wt%;Preferably, the partial size of cassava alcohol slag is less than 0.9mm.
It applies the technical scheme of the present invention, in the presence of alkali, carrying out the first hydrolytic process can be such that cassava alcohol slag sends out Unboiled water solution, to make lignin dissolution into water.In second hydrolytic process, under the action of an acid, the inhibition that the first hydrolysis generates The substance of alcoholic fermentation is reacted with acid to be removed, this can further decrease inhibition of the lignin to fermentation process, so as to Greatly improve the content of ethyl alcohol.
Specific embodiment
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase Mutually combination.Below in conjunction with embodiment, the present invention will be described in detail.
It is existing the method for ethyl alcohol to be prepared using cassava alcohol slag that there are alcohol yieds is low as described in background technique The problem of.In order to solve the above-mentioned technical problem, this application provides a kind of method for preparing ethyl alcohol using cassava alcohol slag, the party Method includes that cassava alcohol slag is carried out the first hydrolytic process, obtains the first solid product under the catalytic action of alkali;In urging for acid Under change effect, the first solid product is subjected to the second hydrolytic process, obtains second solid phase product;And second solid phase product is carried out Diastatic fermentation obtains ethyl alcohol.
Due to containing a large amount of lignin in cassava alcohol slag, the presence of lignin will affect the production of ethyl alcohol in fermentation process Rate.Originally in the presence of alkali, carrying out the first hydrolytic process can be such that cassava alcohol slag hydrolyzes, so that lignin dissolution be made to arrive In water, while some phenolic substancess can also be generated, which, which is adsorbed on the first solid product, can inhibit subsequent Alcoholic fermentation process.In second hydrolytic process, under the action of an acid, above-mentioned phenolic substances reacted with acid after from the first solid product Upper removing obtains second solid phase product.Second hydrolytic process can remove the phenolic substances of above-mentioned inhibition fermentation process, thus After the second hydrolytic process, be conducive to the yield for improving ethyl alcohol in fermentation process.
While the superiority in order to prove processing method in the application, inventor have made intensive studies: first to cassiri Smart slag carries out sour processing, then carries out subsequent alkali process and fermentation process again.It is made using the technique that the method prepares ethyl alcohol Ethanol production it is lower.It traces it to its cause as in sour treatment process, No. 5 positions of lignin G unit can be used as reaction site can be with Condensation reaction occurs for lignin other structures unit, and then generates condensation property lignin, and the generation of condensation property lignin is so that wood Element removing becomes difficult, thus affects the process of alcohol prepared by fermenting.
And as first carried out alkali process to cassava alcohol slag, that most of lignin can be removed by hydrolysis, through the first solid-liquid After separation, the first solid product is obtained.When first solid product to be carried out to the second hydrolytic process under subsequent acidic environment, suppression The phenolic substances of preparing alcohol fermentation is reacted with acid to be removed, so as to reduce its inhibition to fermentation.Thus pass through the second water The content for be conducive to improving ethyl alcohol obtained in fermentation process of solution preocess.It is thus this for cassava alcohol slag to contain wood For the more material of quality, it is clear that the sour treatment effect of progress is more preferable after first carrying out alkali process, this is because in alkali process process Middle lignin can be easier to be removed, so that the hydrolysis of advantageous fibre element enzyme greatly improves the content of ethyl alcohol in turn.
Specifically, the above method includes: cassava alcohol slag to be carried out the first hydrolytic process, so under the catalytic action of alkali First time separation of solid and liquid process is carried out afterwards, obtains the first solid slag (the first solid product) and filtrate;It, will under the catalytic action of acid First solid slag carries out the second hydrolytic process, then carries out second of separation of solid and liquid process, and obtaining the second solid slag, (second solid phase produces Object);And the second solid slag is subjected to diastatic fermentation, obtain ethyl alcohol.
Be conducive to improve the removal rate of lignin using above-mentioned preparation method, while the yield of ethyl alcohol can also be improved.? In a kind of preferred embodiment, method further includes carrying out in the first hydrolytic process to the reaction system of the first hydrolysis The step of heating.
Under base catalysis, cassava alcohol slag is able to carry out initial hydrolysis.In the first hydrolytic process, the first hydrolysis is produced Objects system is heated, and the lignin in above-mentioned solid reactant can be made further to be hydrolyzed, to improve lignin Removal rate, and then be conducive to improve ethyl alcohol yield.
Preferably, the temperature of the first hydrolytic process is 150~220 DEG C, and heating time is 1~2h.First hydrolytic process The heating time of temperature includes but is not limited to above range, and limited be conducive to further increase within the above range it is wooden The removal rate of element.
Preferably, in the first hydrolytic process, alkali is the mixed liquor of the hydrogen peroxide of 30wt% and the ammonium hydroxide of 5~15wt%, double The volume of oxygen water is the 10~20% of the volume of ammonium hydroxide.
In a preferred embodiment, the above method further includes in the second hydrolytic process, to the second hydrolytic process Reaction system the step of being heated.In second of hydrolytic process, the second hydrolysate system is heated, Neng Goujia The extent of reaction of phenols and acid that fast first hydrolytic process generates further improves to improve the removal rate of phenolic substances The yield of ethyl alcohol during diastatic fermentation.
Preferably, the heating temperature of the second hydrolytic process is 160~210 DEG C, and heating time is 40~75min.Second water The heating time of the temperature of solution preocess includes but is not limited to above range, and is limited and be conducive within the above range further Improve the removal rate of phenolic substances in the second hydrolytic process.
Preferably, in the second hydrolytic process, acid is the aqueous sulfuric acid of 3~5wt% of concentration.
During diastatic fermentation, nutriment needed for needing to be added fermenting microbe.Preferably, above-mentioned diastatic fermentation packet Include the process that the mixture of second solid phase product, cellulase, inorganic salts and yeast ferments.
In a preferred embodiment, before carrying out diastatic fermentation process, the above method further include: consolidate second After phase product is washed to neutrality, alcohol is then carried out using ethanol water and washes processing;And using the side of flash distillation after alcohol washes processing Formula recycles ethyl alcohol, obtains second solid phase product.It will further can be obtained through the second hydrolytic process using aforesaid operations process The lignin on solid product surface improve ethyl alcohol during diastatic fermentation so as to further decrease the content of lignin Yield.
It is highly preferred that the volume fraction of the middle ethyl alcohol of ethanol water is 10~20%, temperature is 80~100 DEG C, and alcohol is washed The time for the treatment of process is 30~60min.
In a preferred embodiment, the total amount relative to the reaction system of diastatic fermentation, the addition of cellulase Amount is 15~25FPU/g.Cellulase, which is added, can speed up the percent hydrolysis of cellulose.And the additional amount of cellulase includes but Be not limited to above range, and limited be conducive to further increase within the above range cellulose in fermentation process be hydrolyzed into it is small The percent hydrolysis of molecule carbohydrate, and then improve the yield of ethyl alcohol.
During diastatic fermentation, need to be added nutriment of the inorganic salts as fermenting microbe indispensability.A kind of preferred In embodiment, inorganic salts include but is not limited to urea and diammonium hydrogen phosphate.Preferably, the dosage of urea is 2~4g/L;It is preferred that Ground, the dosage of diammonium hydrogen phosphate are 1~2g/L.
In order to improve the yield of ethyl alcohol during diastatic fermentation, it is preferable that this method further includes during diastatic fermentation The step of supplementing second solid phase product.
Preferably, the number for supplementing second solid phase product is 2~4 times, and the time interval of the adjacent process of feed supplement twice is 24h;
Preferably, after supplementing second solid phase product, the concentration of second solid phase product is 200~260g/L.By diastatic fermentation The concentration of second solid phase product limits within the above range in the process, is conducive to improve fermentation efficiency.
Preferably, the contents on dry basis of lignin is less than 10wt% in second solid phase product, and moisture content is 5~20wt%; Preferably, the partial size of cassava alcohol slag is less than 0.9mm.
The application is described in further detail below in conjunction with specific embodiment, these embodiments should not be understood as limitation originally Apply for range claimed.
The contents on dry basis of lignin is 20%~23wt% in cassava alcohol slag in the embodiment of the present application, and moisture content is 5%-10wt%.
Embodiment 1
It is added in 1L autoclave after weak aqua ammonia is mixed with hydrogen peroxide, wherein the weight percentage of weak aqua ammonia is The volume of the ammonium hydroxide 500mL of 10wt%, hydrogen peroxide (30wt%) are 50mL, obtain lye (pre-treatment solvents).By 60g cassiri Smart slag is added in autoclave, after closed, impregnates cassava alcohol slag in pre-treatment solvents, to carry out the first water Solution preocess, wherein the time impregnated is 3h.After immersion, start temperature program, at 170 DEG C, handles 1h.After treatment Carry out decrease temperature and pressure, after pressure drops to normal pressure, will treated that cassava alcohol slag is filtered, filter residue (the is obtained after filtering One solid product).
Above-mentioned filter residue is put into autoclave, and the dilution heat of sulfuric acid 300mL that weight percentage is 3% is added, To carry out the second hydrolytic process, wherein the temperature of the second hydrolytic process is 150 DEG C, the processing time is 50min.After treatment, After pressure drops to normal pressure, material is released, and carries out the solid material (second for filtering after being washed to neutrality, obtaining after filtering Solid product).It to dry matter is 10wt%, substrate material as fermentation into above-mentioned solid material plus after water.
Urea and diammonium hydrogen phosphate (additive amount is respectively 2g/L, 1g/L) are added into above-mentioned substrate material, then 121 Sterilize 30min at DEG C, after sterilizing, is cooled to room temperature.Cellulase and work are added in material after stating sterilizing then up Saccharomyces cerevisiae after change carries out diastatic fermentation at 35 DEG C, wherein the additional amount of cellulase is 15FPU/g substrate, is added and lives Saccharomyces cerevisiae after change makes the amount of the saccharomyces cerevisiae of fermentation liquid reach 108cfu/mL.Fermentation carries out feed supplement afterwards for 24 hours, and feed supplement is hair The substrate material of ferment.It feed supplement 2 times, is divided into for 24 hours.Make the concentration of substrate of total material in 200g/L, above-mentioned material fermentation after feed supplement After 96h, the concentration of ethyl alcohol can achieve 50g/L.
Embodiment 2
It is added in 1L autoclave after weak aqua ammonia is mixed with hydrogen peroxide, wherein the weight percentage of weak aqua ammonia is The volume of the ammonium hydroxide 500mL of 10wt%, the hydrogen peroxide being added (30wt%) are 100mL, obtain lye (pre-treatment solvents).It will 60g cassava alcohol slag is added in autoclave, after closed, impregnates cassava alcohol slag in pre-treatment solvents, with The first hydrolytic process is carried out, wherein the time impregnated is 4h.After immersion, start temperature program, at 190 DEG C, handles 1h. After treatment carries out decrease temperature and pressure, and after pressure drops to normal pressure, by treated, cassava alcohol slag is filtered, after filtering To filter residue (the first solid product).
Above-mentioned filter residue is put into autoclave, and the dilution heat of sulfuric acid 400mL that weight percentage is 4% is added, To carry out the second hydrolytic process, wherein the temperature of the second hydrolytic process is 170 DEG C, the processing time is 60min.After treatment, After pressure drops to normal pressure, material is released, and carries out the solid material (second for filtering after being washed to neutrality, obtaining after filtering Solid product).It to dry matter is 10wt%, substrate material as fermentation into above-mentioned solid-state material plus after water.
Urea and diammonium hydrogen phosphate (additive amount is respectively 2g/L, 1g/L) are added into above-mentioned substrate material, then 121 Sterilize 30min at DEG C, after sterilizing, after being cooled to room temperature.State then up sterilizing after material in be added cellulase and Saccharomyces cerevisiae after activation carries out diastatic fermentation at 35 DEG C, and cellulase is added, and the additional amount of cellulase is 15FPU/g Substrate, the saccharomyces cerevisiae after activation is added, makes the amount of the saccharomyces cerevisiae of fermentation liquid reach 108Cfu/mL, fermentation are mended afterwards for 24 hours Material, feed supplement be the substrate material to ferment, feed supplement 2 times, are divided into for 24 hours.Make the concentration of substrate of total material in 240g/L after feed supplement, on Material fermentation 120h is stated, the concentration of ethyl alcohol can achieve 60g/L.
Embodiment 3
With the difference of embodiment 1 are as follows: without heating in the first hydrolytic process, the second hydrolytic process is also without heating. The concentration of ethyl alcohol can achieve 21g/L.
Embodiment 4
With the difference of embodiment 1 are as follows: the heating temperature in the first hydrolytic process is 250 DEG C, heating time 1h.Ethyl alcohol it is dense Degree can achieve 28g/L.
Embodiment 5
With the difference of embodiment 1 are as follows: the heating temperature in the second hydrolytic process is 280 DEG C, heating time 1h.Ethyl alcohol it is dense Degree can achieve 30g/L
Embodiment 6
With the difference of embodiment 1 are as follows: lye is sodium hydroxide.The concentration of ethyl alcohol can achieve 24g/L.
Embodiment 7
With the difference of embodiment 1 are as follows: acid solution is the nitric acid of 3~5wt%.The concentration of ethyl alcohol can achieve 33g/L.
Embodiment 8
With the difference of embodiment 1 are as follows: the additional amount of cellulase is 10FPU/g.The concentration of ethyl alcohol can achieve 37g/L.
Embodiment 9
With the difference of embodiment 1 are as follows: the dosage 8g/L of urea.The concentration of ethyl alcohol can achieve 43g/L.
Embodiment 10
With the difference of embodiment 1 are as follows: the dosage of diammonium hydrogen phosphate is 5g/L.The concentration of ethyl alcohol can achieve 40g/L.
Embodiment 11
With the difference of embodiment 1 are as follows: without feed supplement.The concentration of ethyl alcohol can achieve 20g/L.
Embodiment 12
With the difference of embodiment 1 are as follows: the concentration of substrate is 300g/L during diastatic fermentation.The concentration of ethyl alcohol can achieve 42g/L。
Embodiment 13
With the difference of embodiment 1 are as follows: the contents on dry basis of the lignin in cassava alcohol slag is 15wt%, and moisture content is 20wt%.
The concentration of ethyl alcohol can achieve 38g/L.
Embodiment 14
With the difference of embodiment 1 are as follows: it is washed using the solid material that deionized water obtains the second hydrolytic process to neutrality, Then it uses the volume fraction of ethyl alcohol to carry out alcohol for 15% ethanol water and washes journey, recycle second finally by the mode of flash distillation Alcohol obtains second solid phase product.The concentration of ethyl alcohol can achieve 55g/L.
Comparative example 1
The untreated cassava alcohol slag of 20g is put into 300mL triangular flask, adds 100mL water, while urea and phosphorus is added Sour hydrogen diammonium (additive amount is respectively 0.2g and 0.1g), then sterilize at 121 DEG C 30min, after sterilizing, is cooled to room Temperature.Saccharomyces cerevisiae after cellulase and activation are added in material after stating sterilizing then up, carries out saccharification hair at 35 DEG C Ferment, wherein the additional amount of cellulase is 15FPU/g substrate, the saccharomyces cerevisiae 1mL of activation is added, so that the quantity of yeast is not Lower than 108cfu/mL.Fermentation carries out feed supplement afterwards for 24 hours, and feed supplement is the substrate material of fermentation.It feed supplement 2 times, is divided into for 24 hours.Feed supplement Making concentration of alcohol of the concentration of substrate of total material in 200g/L, the 96h post-fermentation mash that ferments afterwards is 17g/L.
It can be seen from the above description that the above embodiments of the present invention realized the following chievements:
Comparing embodiment 1 to 14 and comparative example 1 using method provided by the present application it is found that be conducive to improve the production of ethyl alcohol Amount.
Comparing embodiment 1 and 3 to 5 is it is found that by the limit temperature of the first hydrolytic process and the second hydrolytic process in the application Preferably be conducive to improve the yield of ethyl alcohol in range.
Comparing embodiment 1,7 and 8 is it is found that using the production for being conducive to improve ethyl alcohol in the application lye preferably and acid solution Amount.
Comparing embodiment 1 and 8 to 10 is it is found that be limited to the application for the dosage of cellulase, urea and diammonium hydrogen phosphate Preferably be conducive to improve the yield of ethyl alcohol in range.
Comparing embodiment 1,11 is it is found that feed supplement process is conducive to improve the yield of ethyl alcohol.
Comparing embodiment 1,12 is it is found that the concentration of substrate during diastatic fermentation is limited in the application preferably range Be conducive to improve the yield of ethyl alcohol.
Comparing embodiment 1,13 is it is found that be limited to this for the contents on dry basis of the lignin in cassava alcohol slag and moisture content Be conducive to improve the yield of ethyl alcohol in application preferably range.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (12)

1. a kind of method for preparing ethyl alcohol using cassava alcohol slag, which is characterized in that the described method includes:
Under the catalytic action of alkali, the cassava alcohol slag is subjected to the first hydrolytic process, obtains the first solid product;
Under the catalytic action of acid, first solid product is subjected to the second hydrolytic process, obtains second solid phase product;And
The second solid phase product is subjected to diastatic fermentation, obtains ethyl alcohol.
2. the method according to claim 1, wherein the method also includes in first hydrolytic process, The step of reaction system of first hydrolytic process is heated;
Preferably, the temperature of first hydrolytic process is 150~220 DEG C, and heating time is 1~2h.
3. method according to claim 1 or 2, which is characterized in that the alkali is the hydrogen peroxide and 5~15wt% of 30wt% Ammonium hydroxide mixed liquor, the volume of the hydrogen peroxide is the 10~20% of the ammonium hydroxide volume.
4. according to the method in any one of claims 1 to 3, which is characterized in that the method also includes described second In hydrolytic process, the step of heating to the reaction system of second hydrolytic process;Preferably, second hydrolytic process Heating temperature be 160~210 DEG C, heating time be 40~75min.
5. according to the method described in claim 4, it is characterized in that, the aqueous sulfuric acid that the acid is 3~5wt% of concentration.
6. the method according to claim 1, wherein before carrying out the diastatic fermentation process, the method Further include: after the second solid phase product is washed to neutrality, alcohol is then carried out using ethanol water and washes processing;And described Alcohol recycles ethyl alcohol by the way of flash distillation after washing processing, obtains the second solid phase product.
7. according to the method described in claim 6, it is characterized in that, the volume fraction of the middle ethyl alcohol of the ethanol water is 10 ~20%, temperature is 80~100 DEG C, and the alcohol is used to wash the time for the treatment of process as 30~60min.
8. the method according to claim 1, wherein the diastatic fermentation include by the second solid phase product, The process that the mixture of cellulase, inorganic salts and yeast ferments.
9. according to the method described in claim 8, it is characterized in that, the total amount of the reaction system relative to the diastatic fermentation, The additional amount of the cellulase is 15~25FPU/g.
10. according to the method described in claim 8, it is characterized in that, the inorganic salts are selected from urea and diammonium hydrogen phosphate;
Preferably, the dosage of the urea is 2~4g/L;
Preferably, the dosage of the diammonium hydrogen phosphate is 1~2g/L.
11. the method according to claim 1, wherein the method also includes during the diastatic fermentation The step of supplementing the second solid phase product;
Preferably, the number of the supplement second solid phase product process is 2~4 times, and between the time of the adjacent process of feed supplement twice It is divided into for 24 hours;
Preferably, after supplementing the second solid phase product, the concentration of the second solid phase product is 200~260g/L.
12. the method according to claim 1, wherein the butt of the lignin in the second solid phase product contains Amount is less than 10wt%, and moisture content is 5~20wt%;Preferably, the partial size of the cassava alcohol slag is less than 0.9mm.
CN201810265075.0A 2018-03-28 2018-03-28 Method for preparing ethanol by adopting cassava alcohol residues Active CN110317838B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810265075.0A CN110317838B (en) 2018-03-28 2018-03-28 Method for preparing ethanol by adopting cassava alcohol residues

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810265075.0A CN110317838B (en) 2018-03-28 2018-03-28 Method for preparing ethanol by adopting cassava alcohol residues

Publications (2)

Publication Number Publication Date
CN110317838A true CN110317838A (en) 2019-10-11
CN110317838B CN110317838B (en) 2021-08-03

Family

ID=68109994

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810265075.0A Active CN110317838B (en) 2018-03-28 2018-03-28 Method for preparing ethanol by adopting cassava alcohol residues

Country Status (1)

Country Link
CN (1) CN110317838B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101696427A (en) * 2009-11-03 2010-04-21 天津科技大学 Method for producing fuel ethanol and 2,3-butanediol by using fibrous matter
CN101736630A (en) * 2008-11-21 2010-06-16 安琪酵母股份有限公司 Pretreatment method for biorefinery of lignocellulose
CN102758028A (en) * 2011-04-26 2012-10-31 中国石油大学(北京) Method for pre-treating lignocellulose raw material and for preparing reducing sugar by conversion
CN103045655A (en) * 2012-12-14 2013-04-17 国家海洋局第一海洋研究所 Method for preparing bio-ethanol with suaeda salsa as raw material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101736630A (en) * 2008-11-21 2010-06-16 安琪酵母股份有限公司 Pretreatment method for biorefinery of lignocellulose
CN101696427A (en) * 2009-11-03 2010-04-21 天津科技大学 Method for producing fuel ethanol and 2,3-butanediol by using fibrous matter
CN102758028A (en) * 2011-04-26 2012-10-31 中国石油大学(北京) Method for pre-treating lignocellulose raw material and for preparing reducing sugar by conversion
CN103045655A (en) * 2012-12-14 2013-04-17 国家海洋局第一海洋研究所 Method for preparing bio-ethanol with suaeda salsa as raw material

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
岳军,等: "木薯酒精渣的预处理及补料同步糖化发酵制取乙醇", 《化工进展》 *
徐友海,等: "木薯酒精渣同步糖化发酵制乙醇的工艺研究", 《化工科技》 *

Also Published As

Publication number Publication date
CN110317838B (en) 2021-08-03

Similar Documents

Publication Publication Date Title
Liu et al. Process optimization for deep eutectic solvent pretreatment and enzymatic hydrolysis of sugar cane bagasse for cellulosic ethanol fermentation
Kumar et al. Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review
Bajpai et al. Pretreatment of lignocellulosic biomass
JP7149332B2 (en) Method for producing cellulose, low-dispersion hemicellulose and lignin-dissociated polyphenols from fibrous biomass
CN100999739A (en) Process for treating straw by vapour explosive and alkaline oxydol oxydizing
CN110230228A (en) The method of stalk coproduction cellulosic material, furfural and lignin
CN106191135A (en) Lignocellulose is the biorefinery method of raw material coproduction multi-product
CN102671634B (en) Modified cellulose adsorbent and preparation method as well as application thereof
CN102336728A (en) Method for preparing furfural from fiber pentosan hydrolysate by using composite solid acid as catalyst
CN103993053B (en) A kind of water and the method for ammonia coupling preprocessing biomass
Huang et al. A Review of Lignocellulosic Biomass Pretreatment Technologies
CN110066840A (en) A kind of method that hydro-thermal-microwave coupled ion liquid two-step method lignocellulosic material improves enzymolysis efficiency
KR101417836B1 (en) Apparatus for producing cellulosic biomass hydrolysis material by Two-step acid treatment process and method thereof
WO2018133619A1 (en) Method for producing sugar by hydrolyzing thick mash of agricultural and forest biomass raw material
CN103266148B (en) Preprocessing method capable of effectively improving efficiency of generating fermentable sugar by bamboo cellulose enzyme hydrolysis
CN101235605B (en) Lignocellulose pretreatment method and system with acid recovery
CN110317838A (en) Method for preparing ethanol by adopting cassava alcohol residues
CN103045694B (en) Comprehensive utilization method of lignocellulose biomass
CN111576070A (en) Method for removing poplar lignin by recycling acetic acid-hydrogen peroxide pretreatment liquid
CN110963908A (en) Method for preparing levulinic acid by hydrolyzing cellulose based on molten salt hydrate
CN115747263B (en) Method for improving ethanol yield through mixed cellulose fermentation
CN103045693B (en) Comprehensive utilization method of lignocellulose biomass
CN104387505B (en) The extracting method of xylan in a kind of spirit distiller grain
JP2019129771A (en) Production methods of cellulose-containing materials and production methods of bioethanol, as well as lignin-containing glycerin and production methods thereof
Elgharbawy et al. Advances of bioethanol production using ionic liquids

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant