CN110317722A - A kind of tobacco black shank resistance identification apparatus and identification method - Google Patents
A kind of tobacco black shank resistance identification apparatus and identification method Download PDFInfo
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- CN110317722A CN110317722A CN201910705503.1A CN201910705503A CN110317722A CN 110317722 A CN110317722 A CN 110317722A CN 201910705503 A CN201910705503 A CN 201910705503A CN 110317722 A CN110317722 A CN 110317722A
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- 241000208125 Nicotiana Species 0.000 title claims abstract description 81
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 81
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 239000000725 suspension Substances 0.000 claims abstract description 26
- 238000011081 inoculation Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000012797 qualification Methods 0.000 claims abstract description 5
- 230000004913 activation Effects 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 7
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- 235000010585 Ammi visnaga Nutrition 0.000 claims description 3
- 244000153158 Ammi visnaga Species 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000012549 training Methods 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 2
- 238000009331 sowing Methods 0.000 claims description 2
- 238000011177 media preparation Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 3
- 230000001717 pathogenic effect Effects 0.000 abstract description 2
- 230000008653 root damage Effects 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 6
- 235000019504 cigarettes Nutrition 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 241001002356 Valeriana edulis Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The invention discloses a kind of tobacco black shank resistance identification apparatus and identification methods, are related to disease-resistant crops identification technology field.The method of the present invention is the following steps are included: prepared by black shank bacterium activation, inducing spore suspension, Tobacco Germplasm Resources seedlings samples to be identified infect, counts simultaneously qualification result.The present invention carries out Resistance Identification using water planting tobacco seedlings, the process of identification also carries out in water planting environment, maintain the integrality of plant root, prevent root damage it is unbalanced caused by experimental error, and help to observe incidence and pathogenic process, for being precisely controlled inoculation intensity, the accuracy of enhancing identification Tobacco Germplasm Resources black shank fastness.
Description
Technical field
The invention belongs to disease-resistant crops identification technology fields, identify and fill more particularly to a kind of tobacco black shank resistance
It sets and identification method.
Background technique
Tobacco black shank (PhytophthoraparasiticaIt var.nicotiane is) most crushing on tobacco leaf production
One of disease, be the most important factor for restricting each cigarette district leaf tobacco production in China, flue-cured tobacco, air-curing of tobacco leaves, suncured tabacco, white rib can be endangered
All cultivation tobaccos such as cigarette, Turkish tobaccos.1896, BreddeHaan had found tobacco black shank in Java for the first time.Nineteen twenty-four,
Cook observes this disease in Bo Duoli again.Hereafter, each main production cigarette district in the world reports tobacco black shank successively
Situation of the harm, now the disease is throughout temperate zone, each cigarette district in subtropical zone and the torrid zone.In the 1940s, lesion be expanded to Yellow River basin,
Each production cigarette of the Yangtze river basin and Pearl River Delta saves, and in these areas, tobacco black shank has resulted in heavy losses.According to incomplete system
Meter, onset area is up to 76373hm to China's tobacco black shank every year on average2, 2869.26 ten thousand kg of production loss, quality loss is super
1.23 hundred million yuan are crossed, tobacco virus is only second to.Production practices show that preventing and treating most economical, effective, the environmentally friendly measure of balck shank is exactly
Breeding resistant variety, Resistance Identification become naturally important one in the work such as anti-source digging utilization, breeding for disease resistance, resistance monitoring
Ring.Therefore, accurately and effectively Resistance Identification method is most important to the identification of tobacco resistant material and the breeding of disease-resistant variety
's.
Bacterium paddy inocalation method is mainly used to the identification of the anti-balck shank germplasm materials of tobacco at present.After millet is cooked wherein
Black shank bacterium is cultivated as inoculation source, tobacco seedlings basal part of stem is gently scratched into mouth with pocket knife, is inoculated with 0.5-1g bacterium paddy, 2-3 after inoculation
Week investigation incidence.
That there are problems is as follows for the technology at present: 1, cannot accurately control inoculation intensity by the inoculation of bacterium paddy, therefore cannot be quasi-
Really identify the difference of the anti-balck shank of Tobacco Germplasm Resources；2, since environmental Kuznets Curves accuracy is not high, disease time is too long；3, Bu Nengzhun
Really control inoculation position, mycelia and spore may be infected by root and basal part of stem.
Summary of the invention
The purpose of the present invention is to provide a kind of tobacco black shank resistance identification apparatus and identification methods, for being precisely controlled
It is inoculated with intensity, the accuracy of enhancing identification Tobacco Germplasm Resources black shank fastness.
The present invention is achieved by the following technical solutions:
The present invention be a kind of tobacco black shank resistance identification apparatus and identification method, the identification method the following steps are included:
Black shank bacterium activation:
Oat medium is prepared, and pours into 25mL culture medium in each batch cultur ware；In the culture medium of the culture dish
Middle inoculation black shank bacterium；The culture dish for connecting bacterium is sealed, token types, date are marked；It is placed in 28 DEG C of incubators；Training
Feeding 7d is activated；Expand with the tobacco black shank bacterium of culture to 7d numerous, 28 DEG C of cultures to 14d, progress spore suspension
Preparation；
Spore suspension preparation:
After 15mL sterilizes 0.1% potassium nitrate solution is added in black shank bacterium culture dish；4 DEG C of dark culturing 20min；25 DEG C of light
According to culture 25min；Liquid in culture dish is moved into 50mL centrifuge tube again；Unify spore suspension concentration by blood counting chamber
To 1 × 104A/mL；
Tobacco Germplasm Resources seedlings samples to be identified infect:
Tobacco Germplasm Resources seedlings samples to be identified are placed in floating plate holes, spore suspension is poured into identification apparatus transparent tube
In, the floating plate with Tobacco Germplasm Resources seedlings samples to be identified is placed into, and cover hood, is infected under 25 DEG C of dark surrounds
3h；
Count simultaneously qualification result:
Hood is taken away, and changes the spore suspension in device into clear water after cultivating 5d under 25 DEG C of light environments and counts tobacco seedlings
Incidence, and tobacco black shank resistance situation is identified according to statistical result.
Further, a kind of tobacco black shank resistance identification method, further include sample selection to be identified: tobacco is broadcast
Kind, to Tobacco Germplasm Resources growth of seedling to small cross phase, transplants into water planting seed plate, grow to the Tobacco Germplasm Resources seedling of grand cross phase
It can be used as and infect sample.
Further, the preparation method of the oat medium are as follows: weigh oat 33g and pour into pot, deionized water is added
500ml boils；It boils to oat in after grume, uses filtered through gauze；Filtrate is taken, 1000ml is settled to；18g agar is added；Sterilizing
Obtain oat medium.
Further, the method for the inoculation of medium black shank bacterium of the culture dish are as follows: with punch in the black shin of a ware
It is punched in disease, black shank bacterium is placed on to the center of oat medium using toothpick, convenient for the growth of bacterium.
Further, the method that the Tobacco Germplasm Resources seedlings samples to be identified infect further include: if you need to accurately carry out each portion
Position inoculation, using bracket by floating plate height be fixed, and adjust spore suspension depth make its be dipped to the tobacco seedlings tip of a root,
Root hair, main root or rhizome junction.
A kind of tobacco black shank resistance identification apparatus, including transparent tube, floating plate and hood, the transparent tube is for containing
Spore suspension and clear water are put, the floating plate surface offers several holes, and floating plate week side is equipped with and hole phase
Logical open slot, described hole and open slot are for clamping Tobacco Germplasm Resources seedlings samples to be identified.
Further, a kind of tobacco black shank resistance identification apparatus, further includes bracket, and the bracket is cylinder
Shape.
The invention has the following advantages:
The present invention carries out Resistance Identification using water planting tobacco seedlings, and the process of identification also carries out in water planting environment, maintains plant root
The integrality in portion, prevent root damage it is unbalanced caused by experimental error, and help to observe incidence and pathogenic process.
The present invention is precisely controlled by activation system to black shank bacterium and its spore suspension induction system, Neng Goujing
The really concentration and vigor of control spore suspension, accurate control inoculation intensity, the accuracy of enhancing identification germplasm resistance.
The present invention mainly infects the characteristic of tobacco root and basal part of stem using balck shank, cooperates branch bridge joint by floating plate
Kind, adjustable tobacco seedlings immerse the depth of spore suspension, so as to accurately control inoculation position.
Quickly and efficiently, conidium vitality is strong for spore suspension abductive approach of the invention, can observe tobacco at the 5th day
The difference of germplasm black shank fastness, determination rates are high.
Operation of the present invention is simple and fast, infects environment (illumination, humidity) by glare shield controls black shank bacterium, and control
Inoculation temperature improves infect efficiency, saves qualification time.
Hole is arranged by each floating plate in the present invention, compares and repeats for Disease Resistance Identification, and groove is arranged beside hole,
Convenient for tobacco seedlings are put into and avoid causing to damage to plant.
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, will be described below to embodiment required
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without creative efforts, it can also be obtained according to these attached drawings other attached
Figure.
Fig. 1 is a kind of step flow chart of tobacco black shank resistance identification method of the present invention.
Fig. 2 is a kind of structural schematic diagram of the floating plate of tobacco black shank resistance identification apparatus of the present invention.
Fig. 3 is a kind of structural schematic diagram of the bracket of tobacco black shank resistance identification apparatus of the present invention.
Fig. 4 is that the structure of a kind of transparent tube of tobacco black shank resistance identification apparatus of the present invention, floating plate and hood is shown
It is intended to.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, a kind of tobacco black shank resistance identification method of the invention, comprising the following steps:
One, sample selection to be identified: tobacco sowing is transplanted to Tobacco Germplasm Resources growth of seedling to small cross phase into water planting seed plate,
The Tobacco Germplasm Resources seedling for growing to the grand cross phase, which can be used as, infects sample.
Two, black shank bacterium activates:
It prepares oat medium: weighing oat 33g and pour into pot, deionized water 500ml is added, boils；It boils to oat in sticky
After shape, filtered through gauze is used；Filtrate is taken, 1000ml is settled to；18g agar is added；Sterilizing obtains oat medium；
25mL culture medium is poured into each batch cultur ware；In the inoculation of medium black shank bacterium of culture dish: with punching
Device punches in a ware balck shank, and black shank bacterium is placed on to the center of oat medium using toothpick, convenient for the growth of bacterium；
The culture dish for connecting bacterium is sealed, token types, date are marked；It is placed in 28 DEG C of incubators；Culture 7d lives
Change；Carry out expanding numerous, 28 DEG C of cultures to 14d, progress spore suspension preparation with the tobacco black shank of culture to 7d.
Three, prepared by spore suspension:
After 15mL sterilizes 0.1% potassium nitrate solution is added in black shank bacterium culture dish；4 DEG C of dark culturing 20min；25 DEG C of light
According to culture 25min；Liquid in culture dish is moved into 50mL centrifuge tube again；Unify spore suspension concentration by blood counting chamber
To 1 × 104A/mL.
Four, Tobacco Germplasm Resources seedlings samples to be identified infect:
Tobacco Germplasm Resources seedlings samples to be identified are placed in floating plate holes, spore suspension is poured into identification apparatus transparent tube
In, the floating plate with Tobacco Germplasm Resources seedlings samples to be identified is placed into, and cover hood, is infected under 25 DEG C of dark surrounds
3h；
If you need to accurately carry out the inoculation at each position, floating plate height is fixed using bracket, and adjust spore suspension depth
Degree makes it be dipped to the tobacco seedlings tip of a root, root hair, main root or rhizome junction.
Five, statistics and qualification result:
Hood is taken away, and changes the spore suspension in device into clear water after cultivating 5d under 25 DEG C of light environments and counts tobacco seedlings
Incidence, and tobacco black shank resistance situation is identified according to statistical result.
As in Figure 2-4, a kind of tobacco black shank resistance identification apparatus of the invention, including transparent tube, floating plate and screening
Light shield, for transparent tube for holding spore suspension and clear water, floating plate surface offers hole, floating plate week side be equipped with and hole
The open slot that hole communicates, opening slot number is 6, and circumferentially array opens up, and hole and open slot are to be identified for clamping
Tobacco Germplasm Resources seedlings samples.
Wherein, further include bracket, as shown in figure 3, bracket is cylindrical shape, can be used for supporting floating plate.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (7)
1. a kind of tobacco black shank resistance identification method, it is characterised in that: the following steps are included:
Black shank bacterium activation:
Oat medium is prepared, and pours into 25mL culture medium in each batch cultur ware；In the culture medium of the culture dish
Middle inoculation black shank bacterium；The culture dish for connecting bacterium is sealed, token types, date are marked；It is placed in 28 DEG C of incubators；Training
Feeding 7d is activated；Expand with the tobacco black shank bacterium of culture to 7d numerous, 28 DEG C of cultures to 14d, progress spore suspension
Preparation；
Spore suspension preparation:
After 15mL sterilizes 0.1% potassium nitrate solution is added in black shank bacterium culture dish；4 DEG C of dark culturing 20min；25 DEG C of light
According to culture 25min；Liquid in culture dish is moved into 50mL centrifuge tube again；Unify spore suspension concentration by blood counting chamber
To 1 × 104A/mL；
Tobacco Germplasm Resources seedlings samples to be identified infect:
Tobacco Germplasm Resources seedlings samples to be identified are placed in floating plate holes, spore suspension is poured into identification apparatus transparent tube
In, the floating plate with Tobacco Germplasm Resources seedlings samples to be identified is placed into, and cover hood, is infected under 25 DEG C of dark surrounds
3h；
Count simultaneously qualification result:
Hood is taken away, and changes the spore suspension in device into clear water after cultivating 5d under 25 DEG C of light environments and counts tobacco seedlings
Incidence, and tobacco black shank resistance situation is identified according to statistical result.
2. a kind of tobacco black shank resistance identification method according to claim 1, which is characterized in that further include sample to be identified
Product selection: tobacco sowing transplants into water planting seed plate to Tobacco Germplasm Resources growth of seedling to small cross phase, grows to the grand cross phase
Tobacco Germplasm Resources seedling can be used as and infect sample.
3. a kind of tobacco black shank resistance identification method according to claim 1, which is characterized in that the oat medium
Preparation method are as follows: weigh oat 33g and pour into pot, be added deionized water 500ml, boil；It boils to oat in after grume, uses
Filtered through gauze；Filtrate is taken, 1000ml is settled to；18g agar is added；Sterilizing obtains oat medium.
4. a kind of tobacco black shank resistance identification method according to claim 1, which is characterized in that the training of the culture dish
Support the method that black shank bacterium is inoculated in base are as follows: punched in a ware balck shank with punch, black shank bacterium is placed on using toothpick
The center of oat medium.
5. a kind of tobacco black shank resistance identification method according to claim 1, which is characterized in that the tobacco to be identified
The method that germplasm seedlings samples infect further include: if you need to accurately carry out the inoculation at each position, using bracket by floating plate height into
Row is fixed, and adjusting spore suspension depth makes it be dipped to the tobacco seedlings tip of a root, root hair, main root or rhizome junction.
6. a kind of tobacco black shank resistance identification apparatus, which is characterized in that described including transparent tube, floating plate and hood
For placket for holding spore suspension and clear water, the floating plate surface offers several holes, the floating plate week side set
There are the open slot communicated with hole, described hole and open slot for clamping Tobacco Germplasm Resources seedlings samples to be identified.
7. a kind of tobacco black shank resistance identification apparatus according to claim 6, which is characterized in that it further include bracket, institute
Bracket is stated as cylindrical shape.
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| CN201910705503.1A CN110317722A (en) | 2019-08-01 | 2019-08-01 | A kind of tobacco black shank resistance identification apparatus and identification method |
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| CN103081678A (en) * | 2013-01-14 | 2013-05-08 | 中国农业科学院烟草研究所 | Rapid identification method of tobacco black shank resistance |
| CN203424098U (en) * | 2013-08-14 | 2014-02-12 | 中国烟草总公司郑州烟草研究院 | Floating plate for plant hydroponics |
| CN104152532A (en) * | 2014-08-07 | 2014-11-19 | 贵州省烟草科学研究院 | Method for identifying bacterial wilt resistance of tobacco seedlings |
| CN104745672A (en) * | 2015-03-04 | 2015-07-01 | 河南省农业科学院烟草研究所 | Method for rapidly identifying black shank resistance of tobaccos |
| CN105075698A (en) * | 2015-09-25 | 2015-11-25 | 云南省烟草农业科学研究院 | Tobacco black shank resistance single plant identification method |
| CN105766573A (en) * | 2016-03-08 | 2016-07-20 | 云南省烟草农业科学研究院 | Method for inoculating wound tobacco root system with phytophthora nicotianae |
| CN106613136A (en) * | 2016-11-16 | 2017-05-10 | 云南省烟草农业科学研究院 | Method for screening tobacco plants resistant to tomato spotted wilt virus |
| CN208211124U (en) * | 2017-09-27 | 2018-12-11 | 安徽省农业科学院农业工程研究所 | A kind of plant liquid culture apparatus |
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