Device and method for correcting same gas concentration values in multi-chamber tandem animal metabolism cabins
Technical Field
The invention relates to the technical field of detection of a multi-chamber linkage animal respiration heat measuring device, in particular to a device and a method for correcting the same gas concentration values in a multi-chamber linkage animal metabolism cabin.
Background
At present, when the oxygen consumption and the carbon dioxide of animals are measured internationally by using a closed or open animal breath heat measuring device with multi-chamber row (2-9 chambers); in the process of manufacturing the equipment, the volumes of the respiratory metabolism chambers are not necessarily completely the same, the volumes of the pipelines of the equipment are different, and even if the ventilation volume of each respiratory metabolism chamber is the same, the measured gas concentration value of each respiratory metabolism chamber is different in the actual test time operation process. In order to solve the problems, the breathing metabolism cabins are corrected by methods such as a latin square method, and then coefficients of oxygen consumption and carbon dioxide amount of each metabolism cabin are calculated, so that time and labor are wasted. On the premise of solving the automatic air pressure balance control device in the metabolic cabin of the animal respiratory heat measuring device, the problem of concentration value inconsistency in each respiratory metabolic cabin caused by rapidly correcting multi-chamber row (2-9 chambers) to measure a single gas concentration value is urgently solved. Therefore, in the process of actually measuring the respiratory metabolism gas production of the animals, the concentration value of the gas in each respiratory metabolism cabin is inaccurate, so that a detection system for correcting the same concentration of the gas in the metabolism cabin needs to be designed urgently.
Disclosure of Invention
The present invention aims to provide a device and a method for calibrating the same gas concentration value in a multi-chamber tandem animal metabolism chamber, so as to solve the problem of the prior art that a detection system for calibrating the same gas concentration in the metabolism chamber is needed to be designed.
In order to achieve the purpose, the invention provides the following technical scheme: the device for correcting the same concentration value of gas in the multi-chamber united animal metabolism cabin comprises a carbon dioxide gas steel cylinder, a primary pressure reducing valve, a secondary pressure reducing valve, a first adjustable gas flowmeter, an air inlet pump with a frequency converter, sixteen groups of ball valves, an eight-group suction metabolism cabin, sixteen groups of second adjustable gas flowmeters, sixteen groups of gas converters, a gas analyzer, a data acquisition controller, a computer and an air exhaust pump with a frequency converter, wherein the primary pressure reducing valve is fixed at the mouth of the carbon dioxide gas steel cylinder in a threaded manner, an output port of the primary pressure reducing valve is sequentially communicated and provided with the secondary pressure reducing valve and the first adjustable gas flowmeter through a pipeline, the first adjustable gas flowmeter is communicated and connected with a pipe joint through a pipeline and is provided with the air inlet pump and the eight groups of ball valves in parallel, the other ends of the, the output ports of the eight groups of second adjustable gas flow meters are communicated with the air inlet ends of the eight groups of inhalation metabolism cabins in a one-to-one correspondence manner through pipelines, the exhaust ends of the eight groups of respiration metabolism cabins are communicated with the air inlet ends of the other eight groups of second adjustable gas flow meters through pipelines, the air outlet ends of the eight groups of second adjustable gas flow meters are communicated with the air inlet ends of the other eight groups of ball valves, the air outlet ends of the eight groups of ball valves are communicated with the air inlet end of an exhaust pump through pipelines and pipe joints, the left side circulation interfaces of the eight groups of respiration metabolism cabins are communicated with the air inlet ends of the eight groups of gas converters through pipelines and pipe joints in a one-to-one correspondence manner, the right side circulation interfaces of the eight groups of respiration metabolism cabins are communicated with the air inlet ends of the other eight groups of gas converters through pipelines and pipe joints in a one-to-one correspondence manner, and the eight groups of gas, the gas analyzer is communicated and connected with any one of the respiratory metabolism cabins through a pipeline, the gas analyzer is in signal connection with a data acquisition control instrument, and the data acquisition control instrument is in signal connection with a computer.
Further, the adjusting range of the first adjustable gas flowmeter is 60-600mL/min, and the adjusting range of the second adjustable gas flowmeter is 3-30 mL/min.
Furthermore, the outer sides of the air inlet pump and the exhaust pump are fixedly connected with a sound-proof cover for the pump through a base.
Further, the gas analyzer is any one of a thermal conductivity type gas analyzer, an electrochemical type gas analyzer, and an infrared absorption type gas analyzer.
Further, the first adjustable gas flowmeter is communicated and connected with the ball valve through a 6mm silicone tube.
The method for correcting the same gas concentration value in the multi-chamber tandem animal metabolism cabin comprises the following steps: taking a poultry 6-chamber linkage respiration heat measuring device as an example, after a power supply device is started, a gas analyzer and a data acquisition control instrument are started simultaneously, and the temperature and the humidity are set; then respectively adjusting the measuring ranges of the second adjustable gas flow meters for air intake and exhaust of the 6 respiratory metabolism cabins to 20L/min, and enabling a computer to start recording data of the gas analyzer; after the equipment runs for 1 hour, opening a carbon dioxide gas steel cylinder and a secondary pressure reducing valve, adjusting the measuring range of a connected first adjustable gas flowmeter to 600mL/min, measuring the carbon dioxide concentration% value and the generated carbon dioxide L/min for 24 hours, intercepting data of 4-5 hours, and checking the carbon dioxide concentration% value and the carbon dioxide L/min of each respiratory metabolism cabin; by taking the carbon dioxide concentration value of the first respiratory metabolism cabin as a base number, respectively checking the error value of the carbon dioxide concentration value of each respiratory metabolism cabin and the first respiratory metabolism cabin; on the basis, the flow of a second adjustable gas flowmeter of each respiratory metabolism cabin is finely adjusted; after the second adjustable gas flowmeter is adjusted repeatedly in this way, the measured carbon dioxide value in each respiratory metabolism cabin is consistent; if the measured values of the carbon dioxide values are unified, marks can be made on the corresponding scales of the second adjustable gas flow meter on each respiratory metabolism cabin, and then repeated verification is repeatedly performed for 2-3 times.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, under the condition that no animal exists in the respiratory metabolism chambers, after standard carbon dioxide gas is additionally injected, under the condition that the equipment idles, the flow of the gas flowmeter is adjusted by a fine adjustment method after the carbon dioxide value in each respiratory metabolism chamber is measured, and the measured values of the carbon dioxide in the respiratory metabolism chambers of a plurality of animals are basically the same by adjusting the flow of the flowmeter for a plurality of times.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the structure of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
the device comprises a carbon dioxide gas steel cylinder 1, a primary pressure reducing valve 2, a secondary pressure reducing valve 3, a first adjustable gas flowmeter 4, an air inlet pump 5, a ball valve 6, a breathing metabolism cabin 7, a second adjustable gas flowmeter 8, a gas converter 9, a gas analyzer 10, a data acquisition controller 11, a computer 12 and an exhaust pump 13.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution: the device for correcting the same gas concentration value in the multi-chamber united animal metabolism cabin comprises a carbon dioxide gas steel cylinder 1, a primary pressure reducing valve 2, a secondary pressure reducing valve 3, a first adjustable gas flowmeter 4, an air inlet pump 5 with a frequency converter, sixteen groups of ball valves 6, eight groups of metabolic absorption cabins 7, sixteen groups of second adjustable gas flowmeters 8, sixteen groups of gas converters 9, a gas analyzer 10, a data acquisition controller 11, a computer 12 and an exhaust pump 13 with a frequency converter, wherein the primary pressure reducing valve 2 is fixed at the opening of the carbon dioxide gas steel cylinder 1 in a threaded manner, an output port of the primary pressure reducing valve 2 is sequentially communicated with the secondary pressure reducing valve 3 and the first adjustable gas flowmeter 4 through a pipeline, the first adjustable gas flowmeter 4 is communicated with a pipe joint through a pipeline and is provided with the air inlet pump 5 and the eight groups of ball valves 6 in parallel, the other ends of the eight groups of ball, the output ports of the eight groups of second adjustable gas flow meters 8 are communicated and connected with the gas inlet ends of the eight groups of suction metabolism cabins 7 in a one-to-one correspondence manner through pipelines, the gas outlet ends of the eight groups of suction metabolism cabins 7 are communicated and connected with the gas inlet ends of the other eight groups of second adjustable gas flow meters 8 through pipelines, the gas outlet ends of the eight groups of second adjustable gas flow meters 8 are communicated and connected with the gas inlet ends of the other eight groups of ball valves 6, the gas outlet ends of the eight groups of ball valves 6 are communicated and connected with the gas inlet end of the exhaust pump 13 through pipelines and pipe joints, the left side circulation interfaces of the eight groups of suction metabolism cabins 7 are communicated and connected with the gas inlet ends of the eight groups of gas converters 9 through pipelines and pipe joints in a one-to-one correspondence manner, the gas analyzers 10 are communicated and connected in series between the eight groups of gas converters 9 on both sides through pipelines, the gas analyzer 10 is communicated and connected with any one of the respiratory metabolism cabins 7 through a pipeline, the gas analyzer 10 is in signal connection with a data acquisition control instrument 11, and the data acquisition control instrument 11 is in signal connection with a computer 12.
Wherein the adjusting range of the first adjustable gas flowmeter 4 is 60-600mL/min, and the adjusting range of the second adjustable gas flowmeter 8 is 3-30 mL/min.
The outside of air intake pump 5 and exhaust pump 13 is through base fixedly connected with pump sound-proof housing, and noise interference animal is reduced.
The gas analyzer 10 is any one of a thermal conductivity type gas analyzer, an electrochemical type gas analyzer, and an infrared absorption type gas analyzer.
The first adjustable gas flowmeter 4 is communicated and connected with the ball valve 6 through a 6mm silicone tube.
The method for correcting the same gas concentration value in the multi-chamber tandem animal metabolism cabin comprises the following steps: taking a poultry 6-chamber linkage respiration heat measuring device as an example, after a power supply device is started, a gas analyzer 10 and a data acquisition controller 11 are started simultaneously, and the temperature and the humidity are set; then, the measuring ranges of the second adjustable gas flow meters 8 for air intake and exhaust of the 6 respiratory metabolism cabins 7 are respectively adjusted to 20L/min, so that the computer 12 starts to record the data of the gas analyzer 10; after the equipment runs for 1 hour, opening a carbon dioxide gas steel cylinder 1 and a secondary pressure reducing valve 3, adjusting the measuring range of a connected first adjustable gas flowmeter 4 to 600mL/min, measuring the carbon dioxide concentration% value and the generated carbon dioxide L/min for 24 hours, intercepting data of 4-5 hours, and checking the carbon dioxide concentration% value and the carbon dioxide L/min of each respiratory metabolism cabin 7; by taking the carbon dioxide concentration value of the first respiratory metabolism cabin 7 as a base number, respectively checking the error value of the carbon dioxide concentration value of each respiratory metabolism cabin 7 and the carbon dioxide concentration value of the first respiratory metabolism cabin 7; on the basis, the flow of the second adjustable gas flowmeter 8 of each respiratory metabolism cabin 7 is finely adjusted; after the second adjustable gas flowmeter 8 is repeatedly adjusted in this way, the measured carbon dioxide value in each respiratory metabolism cabin 7 is consistent; if the measured values of the carbon dioxide values are unified, marks can be marked on the corresponding scales of the second adjustable gas flow meter 8 on each respiratory metabolism cabin 7, and then repeated verification is repeatedly performed for 2-3 times.
After starting up, performing a pre-test, and adjusting the second adjustable gas flow meter 8 of No. 1-No. 16 inlet and outlet to 20L/min; using 99.9% carbon dioxide, the air input is 600mL/min, and measuring the carbon dioxide content to obtain the data: error rate before statistics: ± 0.0084, data as follows:
on the basis of the obtained data, the gas inflow of 99.9 percent of carbon dioxide is 600 mL/min; no. 3 and No. 4 are respectively finely adjusted; no. 7 and No. 8 second adjustable gas flow meters 8 to 19L/min; numbers 9, 10; no. 11 and No. 12 second adjustable gas flow meters 8 to 20.5L/min; the following data, statistical error rates, were obtained: the data deviation ranges are: ± 0.0054, and an improvement in accuracy of 35.71% after correction, wherein the reference numerals of the sixteen sets of second adjustable gas flow meters 8 are sequentially labeled from left to right and from top to bottom.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.