CN110305859A - A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue - Google Patents
A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue Download PDFInfo
- Publication number
- CN110305859A CN110305859A CN201910702863.6A CN201910702863A CN110305859A CN 110305859 A CN110305859 A CN 110305859A CN 201910702863 A CN201910702863 A CN 201910702863A CN 110305859 A CN110305859 A CN 110305859A
- Authority
- CN
- China
- Prior art keywords
- bacteria residue
- polyoxin
- kasugarnycin
- added
- acid protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 56
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 229930182764 Polyoxin Natural products 0.000 title claims abstract description 35
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 22
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 20
- 108091005508 Acid proteases Proteins 0.000 claims abstract description 33
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 30
- 239000000843 powder Substances 0.000 claims abstract description 25
- 239000004382 Amylase Substances 0.000 claims abstract description 22
- 102000013142 Amylases Human genes 0.000 claims abstract description 22
- 108010065511 Amylases Proteins 0.000 claims abstract description 22
- 108010059892 Cellulase Proteins 0.000 claims abstract description 22
- 235000019418 amylase Nutrition 0.000 claims abstract description 22
- 229940106157 cellulase Drugs 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- 102000016943 Muramidase Human genes 0.000 claims abstract description 12
- 108010014251 Muramidase Proteins 0.000 claims abstract description 12
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 12
- 229960000274 lysozyme Drugs 0.000 claims abstract description 12
- 239000004325 lysozyme Substances 0.000 claims abstract description 12
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 12
- 150000001413 amino acids Chemical class 0.000 claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims description 28
- 238000002156 mixing Methods 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 21
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 12
- 239000000908 ammonium hydroxide Substances 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- 230000000007 visual effect Effects 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 238000004062 sedimentation Methods 0.000 claims description 4
- 235000019750 Crude protein Nutrition 0.000 claims description 3
- 235000002918 Fraxinus excelsior Nutrition 0.000 claims description 3
- 239000002956 ash Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002910 solid waste Substances 0.000 abstract description 6
- 235000010469 Glycine max Nutrition 0.000 abstract description 4
- 244000068988 Glycine max Species 0.000 abstract description 4
- 238000000855 fermentation Methods 0.000 abstract description 4
- 230000004151 fermentation Effects 0.000 abstract description 4
- 239000001913 cellulose Substances 0.000 abstract description 3
- 229920002678 cellulose Polymers 0.000 abstract description 3
- 239000012452 mother liquor Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 229920002472 Starch Polymers 0.000 abstract description 2
- 241001655322 Streptomycetales Species 0.000 abstract description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 150000003384 small molecules Chemical class 0.000 abstract description 2
- 239000008107 starch Substances 0.000 abstract description 2
- 235000019698 starch Nutrition 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- 125000003835 nucleoside group Chemical group 0.000 abstract 1
- 238000000386 microscopy Methods 0.000 description 5
- 239000003262 industrial enzyme Substances 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000011143 downstream manufacturing Methods 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/38—Nucleosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The present invention relates to a kind of method using kasugarnycin and polyoxin bacteria residue production biological nitrogen, the microorganism mixed fermentation is made of kasugarnycin bacteria residue, polyoxin bacteria residue, beancake powder, calcium carbonate, acid protease, lysozyme, amylase, cellulase etc..The present invention will produce the bacteria residue of kasugarnycin and the generation of polyoxin process, concentrated mother liquor is after multiple enzymatic hydrolysis, what is obtained contains a variety of amino acid, small peptide, nucleosides and other small-molecule substances, enzymolysis process has decomposed remaining starch in bacteria residue, soya-bean cake cellulose and streptomycete mycelia, it is low to solve bacteria residue solid waste utility value in kasugarnycin and polyoxin production process, the problems such as long processing period, pollution indiffusion can be reached, it solves on the spot, fermentation raw material recycles problem, improve the economic value of kasugarnycin and polyoxin bacteria residue as production solid waste, with very big economic value and social value.
Description
Technical field
The technical field that the invention belongs to ferment and digest, and in particular to a kind of to utilize kasugarnycin and polyoxin bacteria residue
The method of production biological nitrogen is main with kasugarnycin and polyoxin bacteria residue, beancake powder especially with a variety of industrial enzymes
The enzymolysis process and biological nitrogen preparation method of raw material.
Background technique
Country's agricultural raw material production and supply at present is insufficient, and raw material long-distance transportation cost directly affects product price, raw
Object nitrogen is after using modern biotechnology to carry out biofermentation processing traditional agricultural and sideline product again through purification, drying, crushing etc.
Biological products obtained after series of processes are a kind of infant industry nitrogen sources, in the industry such as fertilizer, feed, fermentation raw material or agriculture
Industry direction has wide application future.
In China, a large amount of antibiotic bacterium dregs pollution and subsequent processing waste are serious, the existing resource of antibiotic bacterium dregs
It is pollution sources again, reasonably handles extremely important.Biological nitrogen belongs to green product, utilizes the existing various industrial enzyme systems in market
The second decomposition that agent carries out solid waste utilizes, and can increase the productivity effect of manufacturing enterprise, and it is sustainable to be conducive to enhancing pharmaceutical manufacturer
Development.
Summary of the invention
In order to solve the problems in the existing technology, the present invention provides a kind of utilization kasugarnycin and polyoxin bacteria residue
The method for producing biological nitrogen, specially using kasugarnycin and polyoxin bacteria residue, beancake powder as primary raw material, by a variety of enzymes
The biological nitrogen that can be efficiently utilized obtained after preparation continuous enzymolysis.The present invention will produce kasugarnycin and polyoxin mistake
The bacteria residue that journey generates has decomposed bacteria residue Streptomyces mycelia, mycoprotein, cellulose, has increased small point after continuous enzymolysis
Sub- substance classes and content.The present invention solves bacteria residue downstream processing difficulty in kasugarnycin and polyoxin production process, mentions
The high economic value of kasugarnycin and polyoxin bacteria residue as production solid waste, the biological nitrogen of production is with multiple directions
Using future, there is very big economic value and social value.
To achieve the goals above, the technical solution adopted by the present invention is that: it is a kind of utilize kasugarnycin and polyoxin bacterium
The method that slag produces biological nitrogen, raw material is counted by weight ratio, by kasugarnycin bacteria residue 20-55%, polyoxin bacteria residue 20-
35%, beancake powder 5-20%, calcium carbonate 0.1-0.5%, lysozyme 0.05-0.15%, acid protease 0.1-0.5%, cellulase
0.05-0.5%, amylase 0.05-0.3% composition.
Further, the method includes the following steps:
(1) kasugarnycin bacteria residue, polyoxin bacteria residue, calcium carbonate are sequentially added into reactor tank, and ammonium hydroxide adjusts pH to 2.0-3.0
Between, temperature is controlled at 45-50 DEG C, after mixing evenly, acid protease is added, and is stirred to react 2-3h, and Centrifuge A sample precipitates ratio
Example no longer declines, and first stage reaction terminates；
(2) ammonium hydroxide adjusts pH between 5.0-6.0, and temperature is stirred to react 6-10h, mirror naturally, lysozyme is added into reactor tank
Mycelia situation is examined, is existed in the visual field without complete mycelia, acid protease is added again, temperature is controlled at 45-50 DEG C, reacts 2-
3h, Centrifuge A sample sedimentation fraction no longer decline, and second stage reaction terminates；
(3) cellulase, amylase are sequentially added into reactor tank, after mixing evenly, beancake powder is added into batch mixer, are reacted
Material mixes in batch mixer with beancake powder in tank, after mixing, digests 12-24h, interassay total reducing sugar and reduced sugar contain
Amount, cellulase and amylase activity, definite value to be measured is relatively stable, and phase III reaction terminates；
(4) acid protease is added again into material, reacts 4-8h again, will material after reaction, direct sterile pack,
Or it is packed after drying.
Further, it counts according to the weight ratio, in step (1), the acid protease of 0.05-0.2% is added.
Further, it counts according to the weight ratio, in step (2), the acid protease of 0.05-0.1% is added again.
Further, it counts according to the weight ratio, in step (4), the acid protease of 0.05-0.2% is added again.
Further, in each step, the additional amount of raw material meets the requirement of the weight proportion.
The crude protein of the biological nitrogen that the present invention also protects the method to be prepared, obtained biological nitrogen product is
55-75%, there is acid protease activity, and total amino acid is greater than 40%；Content of ashes is less than 10%.
Compared with prior art, technical solution of the present invention have it is following the utility model has the advantages that the present invention will to produce spring thunder mould
After multiple enzymatic hydrolysis, what is obtained contains a variety of amino acid, small peptide, core for element and the bacteria residue of polyoxin process generation, concentrated mother liquor
Glycosides and other small-molecule substances, enzymolysis process have decomposed remaining starch in bacteria residue, soya-bean cake cellulose and streptomycete mycelia.This hair
It is bright by a variety of industrial enzyme digestion reactions, solve the downstream processing of bacteria residue and mother liquor in kasugarnycin and polyoxin production process
The problems such as bacteria residue solid waste utility value is low in difficulty height, long processing period and kasugarnycin and polyoxin production process, energy
Enough reach pollution indiffusion, solve on the spot, fermentation raw material recycles problem, improves kasugarnycin and polyoxin bacteria residue
As the economic value of production solid waste, the biological nitrogen of production has the application future of multiple directions, has very big economic valence
Value and social value.
Specific embodiment
Substantive distinguishing features of the present invention can be embodied from following instance, but these examples are somebody's turn to do only as explanation without limiting
The embodiment of invention.
Embodiment 1
Raw material is by weight ratio are as follows: kasugarnycin bacteria residue 55%, polyoxin bacteria residue 35%, beancake powder 5%, calcium carbonate 0.5%, bacteriolyze
Enzyme 0.15%, acid protease 0.5%, cellulase 0.25%, amylase 0.05%.
Specific steps:
(1) kasugarnycin bacteria residue 55%, polyoxin bacteria residue 35%, calcium carbonate 0.5% are sequentially added into reactor tank, and ammonium hydroxide adjusts pH
To between 2.0-3.0, temperature is controlled at 45-50 DEG C, after mixing evenly, acid protease 0.2% is added, is stirred to react 2-3h, from
Heart sample pellet ratio no longer declines, and first stage reaction terminates；
(2) ammonium hydroxide adjusts pH between 5.0-6.0, and temperature is stirred to react 6- naturally, lysozyme 0.15% is added into reactor tank
10h, microscopy mycelia situation exist without complete mycelia in the visual field, and second stage reaction terminates；
(3) cellulase 0.25% is sequentially added into reactor tank, beans are added into batch mixer after mixing evenly for amylase 0.05%
Cake powder 5%, material after mixing, digests 12-24h, interassay total reducing sugar in batch mixer and beancake powder mixing in reactor tank
With content of reducing sugar, cellulase and amylase activity, definite value to be measured is relatively stable, and phase III reaction terminates；
(4) acid protease 0.3% is added into material, reacts 4-8h again, will material after reaction, direct sterile pack,
Or it is packed after drying.
Embodiment 2
Raw material is by weight ratio are as follows: kasugarnycin bacteria residue 40%, polyoxin bacteria residue 35%, beancake powder 20%, calcium carbonate 0.4%, molten
Bacterium enzyme 0.1%, acid protease 0.5%, cellulase 0.5%, amylase 0.3%.
Specific steps:
(1) kasugarnycin bacteria residue 40%, polyoxin bacteria residue 35%, calcium carbonate 0.4% are sequentially added into reactor tank, and ammonium hydroxide adjusts pH
To between 2.0-3.0, temperature is controlled at 45-50 DEG C, after mixing evenly, acid protease 0.1% is added, is stirred to react 2-3h, from
Heart sample pellet ratio no longer declines, and first stage reaction terminates；
(2) ammonium hydroxide adjusts pH between 5.0-6.0, and temperature is stirred to react 6- naturally, lysozyme 0.10% is added into reactor tank
10h, microscopy mycelia situation exist without complete mycelia in the visual field, and second stage reaction terminates；
(3) cellulase 0.5% is sequentially added into reactor tank, soya-bean cake is added into batch mixer after mixing evenly for amylase 0.3%
Powder 20%, material is in batch mixer and beancake powder mixing in reactor tank, after mixing, digests 12-24h, interassay total reducing sugar and
Content of reducing sugar, cellulase and amylase activity, definite value to be measured is relatively stable, and temperature is improved to 80-85 DEG C, reacts 2- again
3h, phase III reaction terminate；
(4) acid protease 0.4% is added into material, reacts 4-8h again, will material after reaction, direct sterile pack,
Or it is packed after drying.
Embodiment 3
Raw material is by weight ratio are as follows: kasugarnycin bacteria residue 55%, polyoxin bacteria residue 20%, beancake powder 5%, calcium carbonate 0.5%, bacteriolyze
Enzyme 0.05-0.15%, acid protease 0.35-0.45%, cellulase 0.05%, amylase 0.1%.
Specific steps:
(1) kasugarnycin bacteria residue 55%, polyoxin bacteria residue 20%, calcium carbonate 0.5% are sequentially added into reactor tank, and ammonium hydroxide adjusts pH
To between 2.0-3.0, temperature is controlled at 45-50 DEG C, after mixing evenly, acid protease 0.05% is added, is stirred to react 2-3h,
Centrifuge A sample sedimentation fraction no longer declines, and first stage reaction terminates；
(2) ammonium hydroxide adjusts pH between 5.0-6.0, and temperature is stirred to react 6- naturally, lysozyme 0.05% is added into reactor tank
10h, microscopy mycelia situation exist without complete mycelia in the visual field, and acid protease 0.1% is added again, and temperature is controlled in 45-50
DEG C, 2-3h is reacted, Centrifuge A sample sedimentation fraction no longer declines, and second stage reaction terminates；
(3) cellulase 0.05% is sequentially added into reactor tank, beans are added into batch mixer after mixing evenly for amylase 0.1%
Cake powder 5%, material after mixing, digests 12-24h, interassay total reducing sugar in batch mixer and beancake powder mixing in reactor tank
With content of reducing sugar, cellulase and amylase activity, definite value to be measured is relatively stable, and temperature is improved to 80-85 DEG C, then secondary response
2-3h, phase III reaction terminate；
(4) acid protease 0.2-0.3% is added into material, reacts 4-8h again, will material after reaction, it is directly sterile
It is packed after pack, or drying.
Embodiment 4
Raw material is by weight ratio are as follows: kasugarnycin bacteria residue 30%, polyoxin bacteria residue 30%, beancake powder 20%, calcium carbonate 0.1-
0.5%, lysozyme 0.1-0.15%, acid protease 0.35%, cellulase 0.2%, amylase 0.3%.
Specific steps:
(1) kasugarnycin bacteria residue 30%, polyoxin bacteria residue 30%, calcium carbonate 0.1% are sequentially added into reactor tank, and ammonium hydroxide adjusts pH
To between 5.0-6.0, temperature is stirred to react 6-10h, microscopy mycelia feelings naturally, lysozyme 0.1-0.15% is added into reactor tank
Condition, exists without complete mycelia in the visual field, and first stage reaction terminates；
(2) acid protease 0.15% is added into reactor tank, and temperature is controlled at 45-50 DEG C, reacts 2-3h, and Centrifuge A sample precipitates ratio
Example no longer declines, and second stage reaction terminates；
(3) cellulase 0.2% is sequentially added into reactor tank, soya-bean cake is added into batch mixer after mixing evenly for amylase 0.3%
Powder 20%, material is in batch mixer and beancake powder mixing in reactor tank, after mixing, digests 12-24h, interassay total reducing sugar and
Content of reducing sugar, cellulase and amylase activity, definite value to be measured is relatively stable, and temperature is improved to 80-85 DEG C, reacts 2- again
3h, phase III reaction terminate；
(4) acid protease 0.2% is added into material, reacts 4-8h again, will material after reaction, direct sterile pack,
Or it is packed after drying.
Embodiment 5
Raw material is by weight ratio are as follows: kasugarnycin bacteria residue 50-55%, polyoxin bacteria residue 30-35%, beancake powder 10%, calcium carbonate
0.1-0.5%, lysozyme 0.15%, acid protease 0.3%, cellulase 0.1-0.2%, amylase 0.15%.
Specific steps:
(1) kasugarnycin bacteria residue 50-55%, polyoxin bacteria residue 30-35%, calcium carbonate 0.4-0.5% are sequentially added into reactor tank,
Ammonium hydroxide adjusts pH between 5.0-6.0, and temperature is stirred to react 6-10h, microscopy naturally, lysozyme 0.15% is added into reactor tank
Mycelia situation, exists without complete mycelia in the visual field, and first stage reaction terminates；
(2) cellulase 0.1-0.2%, amylase 0.15% are sequentially added into reactor tank, after mixing evenly, are added into batch mixer
Enter beancake powder 10%, material mixes in batch mixer with beancake powder in reactor tank, after mixing, digests 12-24h, interassay
Total reducing sugar and content of reducing sugar, cellulase and amylase activity, definite value to be measured is relatively stable, and second stage reaction terminates；
(3) acid protease 0.3% is added into material, reacts 4-8h again, will material after reaction, direct sterile pack,
Or it is packed after drying.
The crude protein for the product that above embodiments obtain has acid protease activity, total amino acid between 55-75%
It is all larger than 40%, content of ashes is respectively less than 10%, and obtained biological nitrogen product has the application future of multiple directions, has very big
Economic value and social value.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
The change or replacement expected without creative work, should be covered by the protection scope of the present invention.Therefore, of the invention
Protection scope should be with protection scope defined by claims.
Claims (8)
1. a kind of method using kasugarnycin and polyoxin bacteria residue production biological nitrogen, which is characterized in that with kasugarnycin
It is primary raw material with polyoxin bacteria residue, beancake powder, obtains biological nitrogen after a variety of enzyme preparation continuous enzymolysis.
2. the method according to claim 1, wherein raw material is counted by weight ratio, by kasugarnycin bacteria residue 20-
55%, polyoxin bacteria residue 20-35%, beancake powder 5-20%, calcium carbonate 0.1-0.5%, lysozyme 0.05-0.15%, acid protease
0.1-0.5%, cellulase 0.05-0.5%, amylase 0.05-0.3% composition.
3. method according to claim 1 or 2, which comprises the steps of:
(1) kasugarnycin bacteria residue, polyoxin bacteria residue, calcium carbonate are sequentially added into reactor tank, and ammonium hydroxide adjusts pH to 2.0-3.0
Between, temperature is controlled at 45-50 DEG C, after mixing evenly, acid protease is added, and is stirred to react 2-3h, and Centrifuge A sample precipitates ratio
Example no longer declines, and first stage reaction terminates；
(2) ammonium hydroxide adjusts pH between 5.0-6.0, and temperature is stirred to react 6-10h, mirror naturally, lysozyme is added into reactor tank
Mycelia situation is examined, is existed in the visual field without complete mycelia, acid protease is added again, temperature is controlled at 45-50 DEG C, reacts 2-
3h, Centrifuge A sample sedimentation fraction no longer decline, and second stage reaction terminates；
(3) cellulase, amylase are sequentially added into reactor tank, after mixing evenly, beancake powder is added into batch mixer, are reacted
Material mixes in batch mixer with beancake powder in tank, after mixing, digests 12-24h, interassay total reducing sugar and reduced sugar contain
Amount, cellulase and amylase activity, definite value to be measured is relatively stable, and phase III reaction terminates；
(4) acid protease is added again into material, reacts 4-8h again, will material after reaction, direct sterile pack,
Or it is packed after drying.
4. according to the method described in claim 3, in step (1), 0.05- is added it is characterized in that, count according to the weight ratio
0.2% acid protease.
5. according to the method described in claim 3, in step (2), being added again it is characterized in that, count according to the weight ratio
The acid protease of 0.05-0.1%.
6. according to the method described in claim 3, in step (4), being added again it is characterized in that, count according to the weight ratio
The acid protease of 0.05-0.2%.
7. according to the method described in claim 3, it is characterized in that, the additional amount of raw material meets the weight in each step
The requirement of proportion.
8. the biological nitrogen that method of any of claims 1-7 is prepared, which is characterized in that obtained biological nitrogen
The crude protein of plain product is 55-75%, there is acid protease activity, and total amino acid is greater than 40%；Content of ashes is less than 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910702863.6A CN110305859A (en) | 2019-07-31 | 2019-07-31 | A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910702863.6A CN110305859A (en) | 2019-07-31 | 2019-07-31 | A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110305859A true CN110305859A (en) | 2019-10-08 |
Family
ID=68082438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910702863.6A Pending CN110305859A (en) | 2019-07-31 | 2019-07-31 | A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110305859A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
| CN112375693A (en) * | 2020-11-19 | 2021-02-19 | 陕西麦可罗生物科技有限公司 | Method for preparing microbial agent by utilizing natural bioflocculant to pretreat kasugamycin fermentation hyphae |
| CN112408681A (en) * | 2020-11-06 | 2021-02-26 | 陕西麦可罗生物科技有限公司 | Harmless comprehensive treatment process for different agricultural antibiotic fermentation hyphae and high-concentration organic wastewater |
| CN112568225A (en) * | 2020-12-09 | 2021-03-30 | 青岛中达农业科技有限公司 | Bactericide containing kasugamycin fermentation bacterial residues and metalaxyl-M |
| CN113749178A (en) * | 2020-06-02 | 2021-12-07 | 杭州俊丰生物工程有限公司 | Enzyme treatment method of beta-lactam antibiotic and intermediate bacterial dregs thereof |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463320A (en) * | 2007-12-17 | 2009-06-24 | 华北制药集团有限责任公司 | Biological nitrogen, and preparation and use thereof |
| CN101967182A (en) * | 2009-07-27 | 2011-02-09 | 中国科学院成都生物研究所 | Antimicrobial compound and preparation method and application thereof |
| CN103160554A (en) * | 2011-12-12 | 2013-06-19 | 山东方明药业集团股份有限公司 | Erythromycin bacteria residue novel treatment method |
| CN104045202A (en) * | 2014-05-22 | 2014-09-17 | 杭州师范大学 | Integrated autotrophic biological nitrogen removal collaborative chemical phosphorus removal reactor |
| US20150044730A1 (en) * | 2012-03-30 | 2015-02-12 | Shandong Honghe Sunkeen Biotechnology Co. Ltd. | Sugar preparation process by enzymatically hydrolyzing sweet potato dreg |
| CN104828928A (en) * | 2014-02-12 | 2015-08-12 | 同济大学 | Preparation method of filtering material of nitrogen and phosphorus removal bio-filter tank |
| CN105063121A (en) * | 2015-01-20 | 2015-11-18 | 河北圣雪大成制药有限责任公司 | Fermentation culture medium for improving spectinomycin yield, and optimization method thereof |
| CN106942470A (en) * | 2017-03-22 | 2017-07-14 | 南京致润生物科技有限公司 | Biological fermentation feed produced using agricultural wastes and its preparation method and application |
| CN108277161A (en) * | 2017-01-05 | 2018-07-13 | 天方药业有限公司 | Waste thallus processing method in a kind of production of spiramvcin and waste thallus circulation utilization method |
| CN109053238A (en) * | 2018-11-01 | 2018-12-21 | 福建省福抗药业股份有限公司 | A kind of organic fertilizer and preparation method thereof of aureomycin bacteria residue preparation |
| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
| CN113929528A (en) * | 2021-11-17 | 2022-01-14 | 塔里木大学 | Preparation process of fertilizer containing microbial nitrogen |
-
2019
- 2019-07-31 CN CN201910702863.6A patent/CN110305859A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463320A (en) * | 2007-12-17 | 2009-06-24 | 华北制药集团有限责任公司 | Biological nitrogen, and preparation and use thereof |
| CN101967182A (en) * | 2009-07-27 | 2011-02-09 | 中国科学院成都生物研究所 | Antimicrobial compound and preparation method and application thereof |
| CN103160554A (en) * | 2011-12-12 | 2013-06-19 | 山东方明药业集团股份有限公司 | Erythromycin bacteria residue novel treatment method |
| US20150044730A1 (en) * | 2012-03-30 | 2015-02-12 | Shandong Honghe Sunkeen Biotechnology Co. Ltd. | Sugar preparation process by enzymatically hydrolyzing sweet potato dreg |
| CN104828928A (en) * | 2014-02-12 | 2015-08-12 | 同济大学 | Preparation method of filtering material of nitrogen and phosphorus removal bio-filter tank |
| CN104045202A (en) * | 2014-05-22 | 2014-09-17 | 杭州师范大学 | Integrated autotrophic biological nitrogen removal collaborative chemical phosphorus removal reactor |
| CN105063121A (en) * | 2015-01-20 | 2015-11-18 | 河北圣雪大成制药有限责任公司 | Fermentation culture medium for improving spectinomycin yield, and optimization method thereof |
| CN108277161A (en) * | 2017-01-05 | 2018-07-13 | 天方药业有限公司 | Waste thallus processing method in a kind of production of spiramvcin and waste thallus circulation utilization method |
| CN106942470A (en) * | 2017-03-22 | 2017-07-14 | 南京致润生物科技有限公司 | Biological fermentation feed produced using agricultural wastes and its preparation method and application |
| CN109053238A (en) * | 2018-11-01 | 2018-12-21 | 福建省福抗药业股份有限公司 | A kind of organic fertilizer and preparation method thereof of aureomycin bacteria residue preparation |
| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
| CN113929528A (en) * | 2021-11-17 | 2022-01-14 | 塔里木大学 | Preparation process of fertilizer containing microbial nitrogen |
Non-Patent Citations (2)
| Title |
|---|
| 刘学艳等: "生物氮素在阿维菌素发酵中的应用研究", 《河北化工》 * |
| 马玉龙等，: "利用泰乐菌素菌渣生产复合酶制剂及效果研究", 《环境科学与技术》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
| CN113749178A (en) * | 2020-06-02 | 2021-12-07 | 杭州俊丰生物工程有限公司 | Enzyme treatment method of beta-lactam antibiotic and intermediate bacterial dregs thereof |
| CN112408681A (en) * | 2020-11-06 | 2021-02-26 | 陕西麦可罗生物科技有限公司 | Harmless comprehensive treatment process for different agricultural antibiotic fermentation hyphae and high-concentration organic wastewater |
| CN112375693A (en) * | 2020-11-19 | 2021-02-19 | 陕西麦可罗生物科技有限公司 | Method for preparing microbial agent by utilizing natural bioflocculant to pretreat kasugamycin fermentation hyphae |
| CN112568225A (en) * | 2020-12-09 | 2021-03-30 | 青岛中达农业科技有限公司 | Bactericide containing kasugamycin fermentation bacterial residues and metalaxyl-M |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110305859A (en) | A method of biological nitrogen is produced using kasugarnycin and polyoxin bacteria residue | |
| CN102115764B (en) | Method for producing alcohol by taking anaerobic effluent as ingredient water | |
| CN100521961C (en) | Process of producing high-protein microbial feed with plant medicine dregs as material through fermentation | |
| RU2495926C2 (en) | Nutritional additive for alcohol fermentation medium | |
| CN103053794A (en) | Method for preparing prebiotics fermented feed by using bagasse and molasses | |
| CN101603065A (en) | A kind of method of utilizing cellulose complex enzyme system to produce glucose and cellobiose | |
| CN104846034A (en) | Method for improving yield of bioethanol prepared by recycling and reusing cellulose | |
| CN102242157B (en) | Method for producing alcohol with wheat starch | |
| CN102533889A (en) | Method for continuously fermenting lysine | |
| CN102071224B (en) | Method for producing sorbitol and gluconate | |
| CN104726510A (en) | Method for preparing lysine through fermenting | |
| CN102899363B (en) | A kind of preparation method of citric acid fermentation broth | |
| CN109536537A (en) | A kind of industrialized producing technology for accelerating stalk hydrolysis acidification | |
| CN109161508A (en) | A kind of microbiological culture media and the preparation method and application thereof | |
| CN102533890A (en) | Production method of lysine | |
| CN102559794A (en) | Lysine preparing method | |
| CN102352383B (en) | Method for preparing succinic acid from bagasse by fermentation | |
| CN105586367A (en) | Method for conducting fermentative production of citric acid by adding saccharifying enzyme stage by stage based on pH responses | |
| CN107475303B (en) | Safe and environment-friendly biogas preparation method | |
| CN102533891A (en) | Production method of lysine | |
| CN114807269A (en) | Lignocellulose whole-cell saccharification technology adopting oxygen treatment method | |
| CN111334447A (en) | Fermentation process of high-yield cellulase clostridium | |
| CN105154494B (en) | A kind of method that lignocellulosic continuous enzymolysis mentions high glucose concentration | |
| CN103343147A (en) | Method for preparing dibutyl succinate from cassava raw materials | |
| CN101880634A (en) | Method for producing feed yeast by using corn steep liquor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191008 |