CN110301941A - A kind of extracting method of vaginal fluid - Google Patents

A kind of extracting method of vaginal fluid Download PDF

Info

Publication number
CN110301941A
CN110301941A CN201910575271.2A CN201910575271A CN110301941A CN 110301941 A CN110301941 A CN 110301941A CN 201910575271 A CN201910575271 A CN 201910575271A CN 110301941 A CN110301941 A CN 110301941A
Authority
CN
China
Prior art keywords
bottle
sample
vaginal fluid
preservation
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910575271.2A
Other languages
Chinese (zh)
Inventor
曾黎峰
熊文芳
吕正和
陈永洪
杜厚明
杨平平
匡小珠
李丹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangxi Karma Medical Equipment Co Ltd
Original Assignee
Jiangxi Karma Medical Equipment Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangxi Karma Medical Equipment Co Ltd filed Critical Jiangxi Karma Medical Equipment Co Ltd
Priority to CN201910575271.2A priority Critical patent/CN110301941A/en
Publication of CN110301941A publication Critical patent/CN110301941A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0263Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045Devices for taking samples of body liquids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045Devices for taking samples of body liquids
    • A61B2010/0074Vaginal or cervical secretions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Mechanical Engineering (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a kind of extracting methods of vaginal fluid, method includes the following steps: S1: preparing material, material includes sampling pad, saves bottle and cabinet;S2: determining the menstrual period for the person of being sampled, and avoids the time limit of its menstrual period determining sampling;S3: it allows the person of being sampled to dress the underpants for being fixed with sampling pad in defined time limit, vaginal fluid is collected by sampling pad;S4: after collecting expiration of time limit, sampling pad is removed from the underpants for the person of being sampled, and the filter layer of sampling pad is saved in liquid from removing and being moved in the sample preservation liquid saved in bottle on adsorption layer so that the cell adsorbed on filter layer, virus and DNA be made to diffuse to sample;S5: as needed, by the direct inspection of preservation bottle equipped with sample or preservation a period of time in cabinet can be placed into.Sampling pad of the invention easily can be removed or be separated by the simple operations for the person of being sampled.

Description

A kind of extracting method of vaginal fluid
Technical field
The present invention relates to gynaecology's health approach field, specially a kind of extracting method of vaginal fluid.
Background technique
Leukorrhea is vagina secretion, is mixed by vaginal mucosa exudate, cervical canal and endometrial gland juice It closes, is formed related with estrogen action.Under normal circumstances, the quality and quantity of leukorrhea changes with the menstrual cycle.Menstruation is dry After net, leukorrhea amount is few, color is white, in the pasty state.When midcycle ovary will ovulate, since cervix gland secretes vigorous, leukorrhea Increase, transparent, micro- glutinous, egg white sample.After ovulation 2~3 days, leukorrhea becomes muddy, thick glutinous and measure few.It passes through front and back, because of pelvic congestion, Vaginal mucosa exudate increases, and leukorrhea is often increased.
Check to vaginal fluid to be that women's health inspection is highly important, cervix cancer is the most common cancer of women One of disease is a kind of tumour, and pathogenesis is with respect to it is well known that be as caused by human papillomavirus.Polymerase chain Reaction (PCR) checks to be considered most sensitive and accurate in the screening methods of cervix cancer early diagnosis.PCR detection spirit enough It is quick, individual cells can be used and accurately detect cervix cancer.But for this inspection, it is necessary to collect cervical cell.Mesh Before, doctor is often used the typical instrument of brush or scraper such as and is directed toward the end of its cell collection portion, even if skilled practitioner makes Cervical cell is collected with such instrument, it is also possible to be injured vaginal wall or cervix, can be caused supervention infection.
Summary of the invention
The purpose of the present invention is to provide a kind of extracting method of vaginal fluid, sampling pad of the invention can pass through by The simple operations of sampling person are easily removed or are separated, convenient for extracting vaginal fluid.
To achieve the above object, the invention provides the following technical scheme: a kind of extracting method of vaginal fluid, including with Lower step:
S1: preparing material, and the material includes the sampling pad for the person's of being sampled wearing, the preservation equipped with Sample preservation liquid Bottle and for saves save bottle and storage sampling pad cabinet;
S2: determining the menstrual period for the person of being sampled, and avoids the time limit of its menstrual period determining sampling;
S3: it allows the person of being sampled to dress the underpants for being fixed with sampling pad in defined time limit, is received by sampling pad Collect vaginal fluid;
S4: collect expiration of time limit after, sampling pad is removed from the underpants for the person of being sampled, and by the filter layer of sampling pad from Removed on adsorption layer and move to save bottle in sample save liquid in, thus make the cell adsorbed on filter layer, virus and DNA diffuses to sample and saves in liquid;
S5: as needed, by the direct inspection of preservation bottle equipped with sample or preservation a period of time in cabinet can be placed into.
Preferably, according to S1, the sampling pad includes filter layer, adsorption layer and waterproof layer, and the filter layer is by size Vaginal fluid break-through is allowed to cross and prevent the grid of cervical cell break-through from forming simultaneously;
The adsorption layer is placed below filter layer and adsorbs the vaginal fluid that filter layer is crossed in break-through;
The waterproof layer is formed by impermeable material, and moisture is allowed to be discharged by the blade unit, but does not allow moisture logical Blade unit discharge is crossed, to prevent the vaginal fluid of absorption from leaking out, as the material with this feature, can be used by breathing freely Property polyethylene film made of lid, the size of waterproof layer is greater than or equal to the size of adsorption layer, and merges in its edge To adsorption layer.
Preferably, it according to S2, after the time limit is the person's of being sampled menstrual period, chooses 5-7 days and is limited as sampling date.
Preferably, according to S3, the sampling pad may include the attaching components for being attached to waterproof layer rear surface, and attaching components can To be adhered on the inner surface of wearer's underpants, allow sample sampling pad be kept fixed a period of time, to collect including The vaginal fluid of cervical cell.
Preferably, according to S4, the filter layer is formed as a superficial layer of the adsorption layer, and is equipped with a perforation line, with Convenient for separating the filter layer with the adsorption layer, the filter layer of vaginal fluid is adsorbed with from removing and roll from sampling pad Or fold, be then placed in the preservation bottle for saving liquid comprising sample, the sample save liquid can by by alcoholic solution with can The solution for optimizing polymerase chain reaction mixes to obtain, and the example of the alcoholic solution may include ethyl alcohol, methanol etc., and can be excellent The example for changing the solution of polymerase chain reaction may include PBS buffer solution, paraformaldehyde etc..
Preferably, according to S5, the preservation bottle equipped with sample can be fed directly to health examination mechanism, in health examination mechanism In, liquid is saved to sample and is centrifuged, the infection of HPV or STD is then detected for example, by the molecular analysis methods of PCR method, Or the preservation bottle equipped with sample is placed in cabinet and temporarily saves a period of time, it waits to be detected.
Preferably, according to S5, the store method of the preservation bottle equipped with sample are as follows:
A: low-temperature treatment is carried out by the intracorporal cooling module of case preservation bottle storage groove intracorporal to case, makes the intracorporal temperature of case Degree is maintained between 2-8 degree;
B: the filter layer for being adsorbed with the sampling pad of vaginal fluid being separated with adsorption layer, filter layer is rolled or folded, It is then placed in the preservation bottle for saving liquid comprising sample;
C: the preservation bottle in B is placed into cabinet in corresponding placing groove, and will by the jacking block of two sides in placing groove Bottle clip is saved to fasten;
D: closing case lid, convenient for bottle heat preservation is saved, saving the cell in bottle, virus and the activity of DNA with raising.
Preferably, it according to S1, is additionally provided in the cabinet and exempts from fixed slide, dyeing liquor bottle, microscope mirror oil bottle, mirror oil Scavenger bottle wipes paper and PH test paper, and the slot for placing above-mentioned object is offered in cabinet.
Compared with prior art, beneficial effects of the present invention are as follows:
One, sampling pad of the invention easily can be removed or be separated by the simple operations for the person of being sampled, and be easy to use;
Two, the present invention is saved by from removing from sampling pad and rolling or fold, being then placed in filter layer comprising sample In the preservation bottle of liquid, the cell adsorbed on filter layer, virus and DNA can be made to diffuse to sample and saved in liquid, convenient for next Step directly saves liquid sample detection to sample;
Three, the present invention can carry out the guarantor of short time by the way that the preservation bottle equipped with sample to be placed in cabinet to sample It deposits, and the cabinet for preservation can keep constant temperature, the cell diffused to save in liquid, virus and DNA is made to keep activity, So that it when detecting, easily facilitates detection.
Detailed description of the invention
Fig. 1 is the schematic internal view of cabinet of the present invention;
Fig. 2 is the front schematic view of sampling pad of the present invention;
Fig. 3 is the reverse side schematic diagram of sampling pad of the present invention;
Fig. 4 is the side view of sampling pad of the present invention;
Fig. 5 is the sectional view of sampling pad filter layer of the present invention;
In figure: 1- sampling pad, 2- filter layer, 3- adsorption layer, 4- perforation line, 5- waterproof layer, 6- attaching components, 7- adsorbent Layer, 8- cabinet, 9- save bottle, and 10- exempts from fixed slide, 11- dyeing liquor bottle, 12- microscope mirror oil bottle, 13- mirror oil scavenger bottle, 14- wipes paper, 15-PH test paper.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Fig. 1 to Fig. 5 is please referred to, the present invention provides a kind of technical solution: a kind of extracting method of vaginal fluid, including with Lower step:
S1: preparing material, and the material includes the sampling pad 1 for the person's of being sampled wearing, the guarantor equipped with Sample preservation liquid Deposit bottle 9 and for saving the cabinet 8 for saving bottle 9 and storing sampling pad 1;
Preferably, the sampling pad 1 includes filter layer 2, adsorption layer 3 and waterproof layer 5, and the filter layer 2 is permitted by size Perhaps vaginal fluid break-through crosses and prevents the grid of cervical cell break-through from forming simultaneously;
The structure and material needs of the filter layer 2 of sampling pad 1 according to the present invention allow to include in vaginal fluid Cell, virus and DNA are effectively adsorbed and are attached to filter layer 2 and be moved out of in filter layer 2 and put it into sample guarantor It after in liquid storage, is effectively separated from the filter layer 2 in Sample preservation liquid, therefore, filter layer 2 may include at least one absorption Oxidant layer 7, it is preferable that adsorbent layer 7 is the fabric made of the naturally occurring or synthetic fiber with low fineness, it is highly preferred that absorption Oxidant layer 7 is formed by hydrophobic fibre.
Small adsorption space can be provided by the low fibroplastic adsorbent layer 7 of fineness, in particular, by hydrophobicity fibre The adsorbent layer 7 that dimension is formed allows to include micropore that water in vaginal fluid effectively diffuses through fiber, allows simultaneously Cell, virus and DNA are effectively adsorbed in the short space between fiber due to capillarity, in addition, when by hydrophobic Property fibroplastic adsorbent layer 7 when immersing in the Sample preservation liquid being mainly made of alcohol (ethyl alcohol etc.), as polar solvent The micropore that alcohol effectively passes through fiber is spread without being adsorbed by hydrophobic adsorption oxidant layer 7, therefore, when by filter layer 2 from asking for Removed in sample pad 1, be put into save bottle 9 in, and be sent to clinic/commercial laboratory carry out vortex processing when, cell, virus and DNA It can effectively be separated from filter layer 2, when the fiber for constituting adsorbent layer 7 has lower fineness (lesser thickness) When, this advantage of hydrophobic material is more prominent.
The adsorption layer 3 is placed on 2 lower section of filter layer and adsorbs the vaginal fluid that filter layer 2 is crossed in break-through;
Adsorption layer 3 can be formed by high adsorption material, effectively to adsorb the vagina point that filter layer 2 is crossed in break-through Secretion, for example, adsorption layer 3 can be formed by cotton, paper pulp or their combination.Specifically, adsorption layer 3 can be by paper pulp and non- Woven fabric (dust-free paper) is formed, and the supatex fabric has enough spaces to adsorb and store vaginal fluid.
The waterproof layer 5 is formed by impermeable material, and moisture is allowed to be discharged by the blade unit, but does not allow moisture logical Blade unit discharge is crossed, to prevent the vaginal fluid of absorption from leaking out, as the material with this feature, can be used by breathing freely Property polyethylene film made of lid, the size of waterproof layer 5 is greater than or equal to the size of adsorption layer 3, and melts in its edge Close adsorption layer 3.
S2: determining the menstrual period for the person of being sampled, and avoids the time limit of its menstrual period determining sampling;
After having to the equal menstrual periods when checking, have no idea to extract leukorrhea in intermenstrual period, when extraction Between will be after it have passed through at least three days the moon, the Checking on effect only in this way made is more accurate.
Preferably, it after the time limit is the person's of being sampled menstrual period, chooses 5-7 days and is limited as sampling date.
S3: allowing the person of being sampled to dress the underpants for being fixed with sampling pad 1 in defined time limit, by sampling pad 1 come Collect vaginal fluid;
Preferably, the sampling pad 1 may include the attaching components 6 for being attached to 5 rear surface of waterproof layer, and attaching components 6 can be with It is adhered on the inner surface of wearer's underpants, allows sample sampling pad 1 to be kept fixed a period of time, to collect including son The vaginal fluid of cervical cell, while the person of being sampled oneself can be made to remove or separate sampling pad 1.
S4: after collecting expiration of time limit, sampling pad 1 is removed from the underpants for the person of being sampled, and by the filter layer of sampling pad 1 2 from removed on adsorption layer 3 and move to save bottle 9 in sample save liquid in, thus make the cell adsorbed on filter layer 2, virus, And DNA diffuses to sample and saves in liquid;
Preferably, the filter layer 2 is formed as a superficial layer of the adsorption layer 3, and is equipped with a perforation line 4, in order to The filter layer 2 is separated with the adsorption layer 3, the filter layer 2 for being adsorbed with vaginal fluid is removed and rolled from from sampling pad 1 Or fold, it is then placed in the preservation bottle 9 for saving liquid comprising sample,
The sample saves liquid and can be obtained by mixing alcoholic solution with the solution that can optimize polymerase chain reaction, The example of the alcoholic solution may include ethyl alcohol, methanol etc., and the example that can optimize the solution of polymerase chain reaction may include PBS buffer solution, paraformaldehyde etc..
S5: as needed, by the direct inspection of preservation bottle 9 equipped with sample or preservation a period of time in cabinet 8 can be placed into.
Preferably, the preservation bottle 9 equipped with sample can be fed directly to health examination mechanism, it is right in health examination mechanism Sample saves liquid and is centrifuged, then for example, by the infection of the molecular analysis methods detection HPV or STD of PCR method, or will dress There is the preservation bottle 9 of sample to be placed in cabinet 8 and temporarily save a period of time, waits to be detected.
Preferably, the store method of the preservation bottle 9 equipped with sample are as follows:
A: low-temperature treatment is carried out to 9 storage groove of preservation bottle in cabinet 8 by the cooling module in cabinet 8, makes its temperature It is maintained between 2-8 degree, to provide good cryo-conservation environment to be detached from the substance of human body, guarantees the complete of DNA structure Property, convenient for detection;
Preferably, an electrically heated rod can be used to heat it, and by a temperature sensor senses temperature, and will letter It number sends controller to and carries out regulating and controlling temperature;
B: the filter layer 2 for the sampling pad 1 for being adsorbed with vaginal fluid is separated with adsorption layer 3, and filter layer 2 is rolled or rolled over It is folded, it is then placed in the preservation bottle 9 for saving liquid comprising sample;When filter element is rolled or folded, pressure is applied to absorption Oxidant layer 7, and in filter element to be put into the fixed container of cell or when filter element carries out vortex processing, it is applied to adsorbent Pressure on layer 7 is released;If the fiber for constituting adsorbent layer 7 has high resiliency, when the pressure for being applied to adsorbent layer 7 exists It immerses cell to save during solution when being released, due to the elastic force of fiber, adsorbs cell in space between the fibers, disease Poison and DNA can be easily separated;
C: the preservation bottle 9 in B is placed into cabinet 8 in corresponding placing groove, and passes through the jacking block of two sides in placing groove Bottle 9 will be saved to be fixedly clamped;
D: closing case lid, convenient for the heat preservation of bottle 9 is saved, saving the cell in bottle 9, virus and the work of DNA with raising Property, to increase the concentration of sample to be detected in solution, convenient for observation detection.
Preferably, it is additionally provided in the cabinet 8 and exempts from fixed slide 10, dyeing liquor bottle 11, microscope mirror oil bottle 12, mirror oil Scavenger bottle 13 wipes paper 14 and PH test paper 15, and the slot for placing above-mentioned object is offered in cabinet 8.
For vaginal fluid checked in see under sampling, pH measurement, Sample preservation, sample smear, dyeing, oil mirror It examines, using the entire overall process of rear lens maintenance is finished, comprehensive reagent article supply being provided, being examined sending cabinet 8 to health When looking into mechanism, testing staff can quickly be detected sample using the article in cabinet 8.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (8)

1. a kind of extracting method of vaginal fluid, which comprises the following steps:
S1: preparing material, and the material includes the sampling pad (1) for the person's of being sampled wearing, the preservation equipped with Sample preservation liquid Bottle (9) and the cabinet (8) for being used to save preservation bottle (9) and storage sampling pad (1);
S2: determining the menstrual period for the person of being sampled, and avoids the time limit of its menstrual period determining sampling;
S3: allowing the person of being sampled to dress the underpants for being fixed with sampling pad (1) in defined time limit, by sampling pad (1) come Collect vaginal fluid;
S4: after collecting expiration of time limit, sampling pad (1) is removed from the underpants for the person of being sampled, and by the filter layer of sampling pad (1) (2) from removing and being moved in the sample preservation liquid saved in bottle (9) on adsorption layer (3), to make to adsorb on filter layer (2) thin Born of the same parents, virus and DNA diffuse to sample and save in liquid;
S5: as needed, by the directly inspection of the preservation bottle (9) equipped with sample or preservation a period of time in cabinet (8) can be placed into.
2. a kind of extracting method of vaginal fluid according to claim 1, it is characterised in that: according to S1, the sampling Padding (1) includes filter layer (2), adsorption layer (3) and waterproof layer (5), and the filter layer (2) allows vaginal fluid by size Break-through crosses and prevents the grid of cervical cell break-through from forming simultaneously;
The adsorption layer (3) is placed below filter layer (2) and adsorbs the vaginal fluid that filter layer (2) are crossed in break-through;
The waterproof layer (5) is formed by impermeable material, allows moisture to be discharged by the blade unit, but do not allow moisture to pass through Blade unit discharge, as the material with this feature, can be used by gas permeability with preventing the vaginal fluid of absorption from leaking out Lid made of polyethylene film, the size of waterproof layer (5) are greater than or equal to the size of adsorption layer (3), and in its edge It is fused to adsorption layer (3).
3. a kind of extracting method of vaginal fluid according to claim 1, it is characterised in that: according to S2, the time After time limit is the person's of being sampled menstrual period, chooses 5-7 days and limited as sampling date.
4. a kind of extracting method of vaginal fluid according to claim 2, it is characterised in that: according to S3, the sampling Padding (1) may include the attaching components (6) for being attached to waterproof layer (5) rear surface, and attaching components (6) can be adhered to wearer's underpants Inner surface on so that sample sampling pad (1) can be kept fixed a period of time, to collect the vagina including cervical cell Secretion.
5. a kind of extracting method of vaginal fluid according to claim 2, it is characterised in that: according to S4, the filtering Layer (2) is formed as a superficial layer of the adsorption layer (3), and is equipped with a perforation line (4), in order to by the filter layer (2) with Adsorption layer (3) separation is adsorbed with the filter layer (2) of vaginal fluid from removing from sampling pad (1) and rolling or fold, so It is put into the preservation bottle (9) for saving liquid comprising sample afterwards;
The sample saves liquid and can be obtained by mixing alcoholic solution with the solution that can optimize polymerase chain reaction, described The example of alcoholic solution may include ethyl alcohol, methanol etc., and the example that can optimize the solution of polymerase chain reaction may include PBS slow Rush solution, paraformaldehyde etc..
6. a kind of extracting method of vaginal fluid according to claim 1, it is characterised in that: according to S5, can will be equipped with The preservation bottle (9) of sample is fed directly to health examination mechanism, in health examination mechanism, saves solution to cell and is centrifuged, Then the infection of HPV or STD is detected for example, by the molecular analysis methods of PCR method, or the preservation bottle (9) equipped with sample is put It sets and temporarily saves a period of time in cabinet (8), wait to be detected.
7. a kind of extracting method of vaginal fluid according to claim 1, it is characterised in that: according to S5, the dress There is the store method of the preservation bottle (9) of sample are as follows:
A: low-temperature treatment is carried out to preservation bottle (9) storage groove in cabinet (8) by the cooling module in cabinet (8), makes cabinet (8) temperature in is maintained between 2-8 degree;
B: the filter layer (2) for the sampling pad (1) for being adsorbed with vaginal fluid is separated with adsorption layer (3), filter layer (2) is rolled Or fold, it is then placed in the preservation bottle (9) for saving liquid comprising sample;
C: the preservation bottle (9) in B is placed into cabinet (8) in corresponding placing groove, and passes through the jacking block of two sides in placing groove Bottle (9) will be saved to be fixedly clamped;
D: closing case lid, convenient for bottle (9) heat preservation is saved, saving the cell in bottle (9), virus and the work of DNA with raising Property.
8. a kind of extracting method of vaginal fluid according to claim 1, it is characterised in that: according to S1, the cabinet (8) it is additionally provided in and exempts from fixed slide (10), dyeing liquor bottle (11), microscope mirror oil bottle (12), mirror oil scavenger bottle (13), wipes Test paper (14) and PH test paper (15) offer the slot for placing above-mentioned object in cabinet (8).
CN201910575271.2A 2019-06-28 2019-06-28 A kind of extracting method of vaginal fluid Pending CN110301941A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910575271.2A CN110301941A (en) 2019-06-28 2019-06-28 A kind of extracting method of vaginal fluid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910575271.2A CN110301941A (en) 2019-06-28 2019-06-28 A kind of extracting method of vaginal fluid

Publications (1)

Publication Number Publication Date
CN110301941A true CN110301941A (en) 2019-10-08

Family

ID=68078592

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910575271.2A Pending CN110301941A (en) 2019-06-28 2019-06-28 A kind of extracting method of vaginal fluid

Country Status (1)

Country Link
CN (1) CN110301941A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116597987A (en) * 2023-07-13 2023-08-15 广州盛安医学检验有限公司 Female lower genital tract microecology evaluation detection system based on big data

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438626A2 (en) * 1990-01-26 1991-07-31 Cryo-Cell International Inc. Apparatus for preparing an elongate fluid-containing member and related apparatus for removing fluid from such member
CN103602630A (en) * 2013-12-04 2014-02-26 刘玉珍 In vitro culture method of cervical intraepithelial neoplasia cells
WO2016052780A1 (en) * 2014-10-01 2016-04-07 (주)한국티씨엠 Cell collection pad for examining cervical diseases and collection kit comprising same
CN107384873A (en) * 2017-09-05 2017-11-24 成都汇宇生物技术有限公司 The purification process of recombined adhenovirus
CN107926933A (en) * 2018-01-14 2018-04-20 上海赛立维生物科技有限公司 A kind of cell-preservation liquid
CN208013093U (en) * 2018-01-29 2018-10-26 深圳海柔思生物医学科技有限公司 Vaginal fluid visible component detection reagent external member
CN109219395A (en) * 2017-05-08 2019-01-15 Tcm生物科学股份有限公司 For collecting the device of the vaginal fluid sample containing cervical cell
CN109221088A (en) * 2018-09-30 2019-01-18 成都远山原力生物科技有限公司 A kind of cell cryopreservation solution, sodium alginate gel, Cellular gels, preparation method, application method and its application
CN109644985A (en) * 2018-12-19 2019-04-19 易普森智慧健康科技(深圳)有限公司 A kind of cell-preservation liquid and its application

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438626A2 (en) * 1990-01-26 1991-07-31 Cryo-Cell International Inc. Apparatus for preparing an elongate fluid-containing member and related apparatus for removing fluid from such member
CN103602630A (en) * 2013-12-04 2014-02-26 刘玉珍 In vitro culture method of cervical intraepithelial neoplasia cells
WO2016052780A1 (en) * 2014-10-01 2016-04-07 (주)한국티씨엠 Cell collection pad for examining cervical diseases and collection kit comprising same
CN106794010A (en) * 2014-10-01 2017-05-31 Tcm韩国股份有限公司 Cell collecting pad and collection set group comprising the collecting pad for checking cervix diseases
CN109219395A (en) * 2017-05-08 2019-01-15 Tcm生物科学股份有限公司 For collecting the device of the vaginal fluid sample containing cervical cell
CN107384873A (en) * 2017-09-05 2017-11-24 成都汇宇生物技术有限公司 The purification process of recombined adhenovirus
CN107926933A (en) * 2018-01-14 2018-04-20 上海赛立维生物科技有限公司 A kind of cell-preservation liquid
CN208013093U (en) * 2018-01-29 2018-10-26 深圳海柔思生物医学科技有限公司 Vaginal fluid visible component detection reagent external member
CN109221088A (en) * 2018-09-30 2019-01-18 成都远山原力生物科技有限公司 A kind of cell cryopreservation solution, sodium alginate gel, Cellular gels, preparation method, application method and its application
CN109644985A (en) * 2018-12-19 2019-04-19 易普森智慧健康科技(深圳)有限公司 A kind of cell-preservation liquid and its application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116597987A (en) * 2023-07-13 2023-08-15 广州盛安医学检验有限公司 Female lower genital tract microecology evaluation detection system based on big data
CN116597987B (en) * 2023-07-13 2024-01-26 广州盛安医学检验有限公司 Female lower genital tract microecology evaluation detection system based on big data

Similar Documents

Publication Publication Date Title
US6627790B2 (en) Sanitary napkin and method for collecting samples of bodily substances
CA2255716C (en) Method and apparatus for collecting vaginal fluid and exfoliated vaginal cells for diagnostic purposes
US4435507A (en) Process and device for preparation of cell samples for cytological tests
US4232673A (en) Method for collecting and processing body fluids
CN110301941A (en) A kind of extracting method of vaginal fluid
EP3427672B1 (en) Apparatus for collecting specimen such as vaginal discharge including cervical cell
CN105929148B (en) A kind of passive type body fluid sample examination based on paper diaper and verifying attachment and application method
CN105675352A (en) Tear fluid collector
US20050215924A1 (en) Noninvasive device for collecting bio-specimen on gynaecology
WO2016052780A1 (en) Cell collection pad for examining cervical diseases and collection kit comprising same
KR100524665B1 (en) Apparatus for gathering the cervical cells
JP4534388B2 (en) Menstrual or vaginal secretion collection device, kit and method
GB2061115A (en) Sanitary napkin
CN210749285U (en) Novel endocrine hydrops is gathered device
CN213121260U (en) Protective pad and kit for collecting vaginal discharge
CN111999124A (en) Protective pad and kit for collecting vaginal discharge and collecting, separating and detecting method
KR101901084B1 (en) A collection device for vaginal secretions including cervical cells
CN214750358U (en) Novel immunochromatography saliva detection kit
WO2000049948A2 (en) Urine collector
KR20060001380A (en) Pad for collecting the cervical cells
WO2019210852A1 (en) Sampling sanitary napkin and use thereof
AU773608B2 (en) Sanitary napkin and method for collecting samples of bodily substances
GB2349573A (en) Method and apparatus for collecting vaginal fluid and exfoliated vaginal cells for diagnostic purposes
CN102042924B (en) A kind of cell or the dyeing of antibacterial, flaking method
KR20120100216A (en) Pad for taking cevical cells

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20191008