CN110301355A - A kind of Chinese cymbidium method for tissue culture - Google Patents
A kind of Chinese cymbidium method for tissue culture Download PDFInfo
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- CN110301355A CN110301355A CN201910701743.4A CN201910701743A CN110301355A CN 110301355 A CN110301355 A CN 110301355A CN 201910701743 A CN201910701743 A CN 201910701743A CN 110301355 A CN110301355 A CN 110301355A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
A kind of Chinese cymbidium method for tissue culture, acquisition, disinfection, Fiber differentiation, Multiplying culture including lateral bud, differentiation culture, culture of rootage, acclimatization and transplants these steps.It has the advantages that the lateral bud explant of one, Chinese cymbidium tissue culture and inducement is obtained under relatively stable, clean greenhouse, and the disinfection pre-treating method for being directly entered gnotobasis after the drying and materials before being drawn materials is conducive to reduce pollution rate；Two, the lateral bud explant that Chinese cymbidium tissue culture and inducement is selected is the two generation buds newly grown, and growth bacterium is few, energetic, conducive to the induction of rhizomes, improves induction success rate；Three, the rhizomes material of 0.32~0.38cm of rugosity φ, 3.5~5.5cm of length after Chinese cymbidium tissue culture rhizomes differentiation selection is proliferated, differentiation capability is strong, is conducive to improve differentiation rate, obtains healthy and strong differentiation seedling.
Description
Technical field
The present invention relates to plant tissue culture technical fields, refer in particular to a kind of Chinese cymbidium method for tissue culture.
Background technique
Chinese cymbidium (Cymbidium sinense) also known as year-report orchid are orchid family (Orchidaceae) Cymbidiums (Cymbidium)
Terrestrial orchid originates in the ground such as China Taiwan, south Fujian, Guangdong, Guangxi, is one of seven major class of China blue.The leaf morphology of Chinese cymbidium
Elegant uniqueness, inflorescence is upright, and flower amount is big, and pattern is plain, and petal delicate fragrance overflows, and has the laudatory title of " spending middle gentleman ".Meanwhile Chinese cymbidium
Root also has certain medicinal efficacy of relievining asthma, and flower also can extract essential oil, make some cosmetics, ornamental, medicinal etc. to collect
It is worth the maximum state's orchid species class of China's export amount of one.
Currently, market tradition Chinese cymbidium seedling breeding is mainly to excavate and a large amount of of wild orchid using plant division breeding seedling
The features such as mode carries out, but there are germ plasm resources to destroy, division propagation coefficient is small, growth cycle is slow, far from satisfaction expansion
Current situation can be effectively relieved in the orchid market demand, Plant Tissue Breeding.Currently, Chinese cymbidium Study on tissue culture mostly use greatly with
Chinese cymbidium seed is that explant carries out aseptic seeding breeding seedling, but the seedling for using seed to be bred is seedling, hereditary
Character can separate, and stability is poor, and seedling consistency is poor, not can apply on some famous-particular-excellent kind Plantlet in vitro；
It has been reported that and rhizomes, stem apex and bud etc. is used to carry out tissue cultures induction for explant, but there is pollution height, induce success rate
The problems such as low；It is that explant carries out seedling tissue-culturing rapid propagation using lateral bud, can get the consistent seedling of a large amount of inhereditary feature height, but
It is had not been reported on Chinese cymbidium.In addition, in Chinese cymbidium scale tissue cultures, since rhizomes breaks up material selection, differentiation speed
Degree speed is different, it is irregular to be differentiated to form seedling.Therefore, induction pollution rate is reduced, induction success rate is promoted and improves root shape
The seedling quality of stem differentiation is particularly important for the tissue cultures of Chinese cymbidium.
Summary of the invention
The present invention provides a kind of Chinese cymbidium method for tissue culture, and main purpose, which is to overcome in existing Chinese cymbidium tissue culture technology, to lure
The defect that pollution rate is high, induction success rate is low, differentiation seedling is irregular is led, provides technology branch for the high quality seedling breeding of Chinese cymbidium
Support.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
A kind of Chinese cymbidium method for tissue culture, comprising the following steps:
1) acquisition of lateral bud: selecting 2~triennial no disease and pests harm, the Chinese cymbidium maternal plant that grows fine is planted, until the Chinese cymbidium is female
Strain lateral bud grow to specification be 5~10cm when；
2) it sterilizes: the lateral bud that specification in step 1 is 5~10cm being dried in 2~3 days in advance, cut the lateral bud later, and will
The lateral bud is placed in gnotobasis the processing that carries out disinfection；
3) it Fiber differentiation: by the Chinese cymbidium lateral bud after being disinfected in step 2, is inoculated in rhizomes induced medium and is trained
It supports；The induced medium are as follows: 1/2MS+6-BA 0.5~1.0mg/L+NAA 2.0~3.0mg/L+ active carbon 1g/L,
pH5.8；Condition of culture: 23~25 DEG C of temperature, 1000~1500 lux of light intensity, light application time is 16 h/d；
4) Multiplying culture: the rhizomes that step 3 Fiber differentiation goes out is cut into length 2~3cm/ item with aseptic operation knife, then is transferred
Into proliferated culture medium；The proliferated culture medium are as follows: MS+6-BA 1.0~3.0mg/L+NAA 0.3~0.5mg/L+ activity
Charcoal 1g/L+ banana puree 20g/L, pH5.8；Condition of culture: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is
16 h/d；
5) differentiation culture: the root that the rugosity of Multiplying culture is 0.32~0.38cm of φ in selecting step 4, length is 3.5~5.5cm
Shape stem, and be forwarded in differential medium, 9~11/bottle of inoculum density；The differential medium are as follows: MS+6-BA 1.0~
3.0mg/L+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 50g/L, pH5.8；Condition of culture: temperature 23~25
DEG C, 1500~1800 lux of light intensity, light application time is 16 h/d；
6) culture of rootage: the specification broken up in step 5 is cut with aseptic operation knife and is the seedling of 3~5cm high, and is forwarded to and takes root
In culture medium, 10~12 seedlings of inoculum density/bottle；Meanwhile the differentiation for cutting away the rhizomes after seedling and being forwarded to again step 5 being trained
It supports in base；The root media are as follows: 1/2MS+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 80g/L, pH5.8；
Condition of culture: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is 16 h/d；
7) acclimatization and transplants: when the test tube seedling of culture of rootage in step 6 it is long to 7~9cm high, root 3~5 when, which is put
The lower refining seedling in seeding room, hardening hot-house culture condition: 23~25 DEG C of temperature, 1800 ~ 2000 lux of light intensity, light application time 16
h/d；After hardening 5 days, take out tissue culture rooted seedling, reject and be attached to the culture medium of root, then transplant to air humidity 75%~85%,
It is grown in the greenhouse that 20~25 DEG C of temperature.
Further, in step 1, the acquisition of the lateral bud specifically includes the following steps: select 2~triennial no disease and pests harm,
After the Chinese cymbidium maternal plant to grow fine, the old matrix in fundatrix strain root is 1. trembled；Disappeared in 1% potassium permanganate 2. planting the maternal plant
Poison drain after pine bark: in perlite=4:1 mixed-matrix；After plantation, the exposed 0.5~1cm of the reed of the maternal plant；3. will
The maternal plant planted moves to indoor greenhouse growth, manages moisture using alternation of wetting and drying method, and moisture pouring is carried out in a manner of pouring root, to
Lateral bud grow to specification be 5~10cm high when, it is spare；Indoor greenhouse condition of culture: temperature is 18~28 DEG C, illumination 4500~
6000lux, light application time 16h/d.
Further, in step 2, the disinfection treatment specifically: the lateral bud cut is placed in 75% dehydrated alcohol and is handled
During which 1min is transferred to rapidly super-clean bench；10~12min, abandoning will be sterilized by 0.1% mercuric chloride of treated the lateral bud of dehydrated alcohol 3
Mercuric chloride, then by the lateral bud with rinsed with sterile water 5~6 times, the lateral bud surface moisture finally is blotted with the filter paper sterilized, it is spare.
Further, it in step 7, takes out the tissue culture rooted seedling and is planted in pine after rejecting is attached to the culture medium of root
Bark: perlite volume ratio is in the mixed-matrix of 4:1, in 20~25 DEG C of air humidity 75%~85%, temperature greenhouses
Growth.
Compared to the prior art, the beneficial effect that the present invention generates is:
The present invention has the advantage that the lateral bud explant of one, Chinese cymbidium tissue culture and inducement is obtained under relatively stable, clean greenhouse
, and the disinfection pre-treating method for being directly entered gnotobasis after the drying and materials before draw materials, it is conducive to reduce dirt
Dye rate；Two, the lateral bud explant that Chinese cymbidium tissue culture and inducement is selected is the two generation buds newly grown, and growth bacterium is few, energetic, is conducive to root
The induction of shape stem improves induction success rate；Three, Chinese cymbidium tissue culture rhizomes differentiation selection proliferation after 0.32~0.38cm of rugosity φ,
The rhizomes material of 3.5~5.5cm of length, differentiation capability is strong, is conducive to improve differentiation rate, obtains healthy and strong differentiation seedling.
Detailed description of the invention
Fig. 1 is that Chinese cymbidium rhizomes induces schematic diagram in embodiment one.
Fig. 2 is that Chinese cymbidium rhizomes is proliferated schematic diagram in embodiment one.
Fig. 3 is that Chinese cymbidium rhizomes breaks up schematic diagram in embodiment one.
Specific embodiment
Illustrate a specific embodiment of the invention with reference to the accompanying drawings.
Embodiment one
Referring to FIG. 1, FIG. 2 and FIG. 3.A kind of Chinese cymbidium method for tissue culture is the packet using the lateral bud of Chinese cymbidium " state perfume (or spice) tree peony " as explant
Include following steps:
1) acquisition of lateral bud: selecting 2~triennial no disease and pests harm, after the Chinese cymbidium maternal plant that grows fine, and it is old 1. to tremble fundatrix strain root
Matrix；2. by pine bark of the maternal plant plantation after being drained with the disinfection of 1% potassium permanganate: in perlite=4:1 mixed-matrix；
After plantation, the exposed 0.5~1cm of the reed of the maternal plant；3. the maternal plant planted is moved to indoor greenhouse growth, using alternation of wetting and drying
Method manages moisture, and moisture pouring is carried out in a manner of pouring root, spare when it is 5~8cm high that lateral bud, which grows to specification,；Indoor greenhouse
Condition of culture: temperature is 18~28 DEG C, 4500~6000lux of illumination, light application time 16h/d；
2) it sterilizes pre-treatment: the lateral bud that specification in step 1 is 5~8cm being dried in 2~3 days in advance, cut the side later
Bud, and the lateral bud is placed in 75% dehydrated alcohol and handles 1min, it is during which transferred to super-clean bench rapidly and carries out disinfection operation；
3) materials disinfection: 0.1% mercuric chloride of step 2 treated lateral bud is sterilized into 10~12min, abandons mercuric chloride, then the lateral bud is used
Rinsed with sterile water 5~6 times, the lateral bud surface moisture finally is blotted with the filter paper sterilized, it is spare；
4) referring to Fig.1.Fiber differentiation: the Chinese cymbidium lateral bud after disinfecting in step 3 is inoculated in rhizomes induced medium
It is cultivated；The induced medium are as follows: 1/2MS+6-BA 0.5~1.0mg/L+NAA 2.0~3.0mg/L+ active carbon
1g/L, pH5.8；Condition of culture: 23~25 DEG C of temperature, 1000~1500 lux of light intensity, light application time is 16 h/d；
5) referring to Fig. 2.Multiplying culture: the rhizomes that step 4 Fiber differentiation goes out is cut into 2~3cm/ of length with aseptic operation knife
Item, then be forwarded in proliferated culture medium；The proliferated culture medium are as follows: 1.0~3.0mg/L of MS+6-BA+NAA 0.3~
0.5mg/L+ active carbon 1g/L+ banana puree 20g/L, pH5.8；Condition of culture: 23~25 DEG C of temperature, light intensity 1500~1800
Lux, light application time are 16 h/d；
6) referring to Fig. 3.Differentiation culture: in selecting step 5 rugosity of Multiplying culture be 0.32~0.38cm of φ, length be 3.5~
The rhizomes of 5.5cm, and be forwarded in differential medium, 9~11/bottle of inoculum density；The differential medium are as follows: MS+6-
BA 1.0~3.0mg/L+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 50g/L, pH5.8；Condition of culture: temperature
23~25 DEG C, 1500~1800 lux of light intensity, light application time is 16 h/d；
7) culture of rootage: the specification broken up in step 6 is cut with aseptic operation knife and is the seedling of 3~5cm high, and is forwarded to and takes root
In culture medium, 10~12 seedlings of inoculum density/bottle；Meanwhile the differentiation for cutting away the rhizomes after seedling and being forwarded to again step 6 being trained
It supports in base；The root media are as follows: 1/2MS+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 80g/L, pH5.8；
Condition of culture: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is 16 h/d；
8) acclimatization and transplants: when the test tube seedling of culture of rootage in step 7 it is long to 7~9cm high, root 3~5 when, which is put
The lower refining seedling in seeding room, hardening hot-house culture condition: 23~25 DEG C of temperature, 1800 ~ 2000 lux of light intensity, light application time 16
h/d；After hardening 5 days, takes out tissue culture rooted seedling and be planted in pine bark: perlite volume after rejecting is attached to the culture medium of root
Than being grown in 20~25 DEG C of air humidity 75%~85%, temperature greenhouses in the mixed-matrix for 4:1.
Embodiment two
A kind of Chinese cymbidium method for tissue culture is using the lateral bud of Chinese cymbidium " chalk " as explant, comprising the following steps:
1) acquisition of lateral bud: selecting 2~triennial no disease and pests harm, after the Chinese cymbidium maternal plant that grows fine, and it is old 1. to tremble fundatrix strain root
Matrix；2. by pine bark of the maternal plant plantation after being drained with the disinfection of 1% potassium permanganate: in perlite=4:1 mixed-matrix；
After plantation, the exposed 0.5~1cm of the reed of the maternal plant；3. the maternal plant planted is moved to indoor greenhouse growth, using alternation of wetting and drying
Method manages moisture, and moisture pouring is carried out in a manner of pouring root, spare when it is 7~10cm high that lateral bud, which grows to specification,；Indoor greenhouse
Condition of culture: temperature is 18~28 DEG C, 4500~6000lux of illumination, light application time 16h/d；
2) it sterilizes pre-treatment: the lateral bud that specification in step 1 is 7~10cm being dried in 2~3 days in advance, cut the side later
Bud, and the lateral bud is placed in 75% dehydrated alcohol and handles 1min, it is during which transferred to super-clean bench rapidly and carries out disinfection operation；
3) materials disinfection: 0.1% mercuric chloride of step 2 treated lateral bud is sterilized into 10~12min, abandons mercuric chloride, then the lateral bud is used
Rinsed with sterile water 5~6 times, the lateral bud surface moisture finally is blotted with the filter paper sterilized, it is spare；
4) it Fiber differentiation: by the Chinese cymbidium lateral bud after being disinfected in step 3, is inoculated in rhizomes induced medium and is trained
It supports；The induced medium are as follows: 1/2MS+6-BA 0.5~1.0mg/L+NAA 2.0~3.0mg/L+ active carbon 1g/L,
pH5.8；Condition of culture: 23~25 DEG C of temperature, 1000~1500 lux of light intensity, light application time is 16 h/d；
5) Multiplying culture: the rhizomes that step 4 Fiber differentiation goes out is cut into length 2~3cm/ item with aseptic operation knife, then is transferred
Into proliferated culture medium；The proliferated culture medium are as follows: MS+6-BA 1.0~3.0mg/L+NAA 0.3~0.5mg/L+ activity
Charcoal 1g/L+ banana puree 20g/L, pH5.8；Condition of culture: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is
16 h/d；
6) differentiation culture: the root that the rugosity of Multiplying culture is 0.32~0.38cm of φ in selecting step 5, length is 3.5~5.5cm
Shape stem, and be forwarded in differential medium, 9~11/bottle of inoculum density；The differential medium are as follows: MS+6-BA 1.0~
3.0mg/L+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 50g/L, pH5.8；Condition of culture: temperature 23~25
DEG C, 1500~1800 lux of light intensity, light application time is 16 h/d；
7) culture of rootage: the specification broken up in step 6 is cut with aseptic operation knife and is the seedling of 3~5cm high, and is forwarded to and takes root
In culture medium, 10~12 seedlings of inoculum density/bottle；Meanwhile the differentiation for cutting away the rhizomes after seedling and being forwarded to again step 6 being trained
It supports in base；The root media are as follows: 1/2MS+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 80g/L, pH5.8；
Condition of culture: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is 16 h/d；
8) acclimatization and transplants: when the test tube seedling of culture of rootage in step 7 it is long to 7~9cm high, root 3~5 when, which is put
The lower refining seedling in seeding room, hardening hot-house culture condition: 23~25 DEG C of temperature, 1800 ~ 2000 lux of light intensity, light application time 16
h/d；After hardening 5 days, takes out tissue culture rooted seedling and be planted in pine bark: perlite volume after rejecting is attached to the culture medium of root
Than being grown in 20~25 DEG C of air humidity 75%~85%, temperature greenhouses in the mixed-matrix for 4:1.
The above is only a specific embodiment of the present invention, but the design concept of the present invention is not limited to this, all to utilize this
Design makes a non-material change to the present invention, and should all belong to behavior that violates the scope of protection of the present invention.
Claims (4)
1. a kind of Chinese cymbidium method for tissue culture, which comprises the following steps:
1) acquisition of lateral bud: selecting 2~triennial no disease and pests harm, the Chinese cymbidium maternal plant that grows fine is planted, until the Chinese cymbidium is female
Strain lateral bud grow to specification be 5~10cm when；
2) it sterilizes: the lateral bud that specification in step 1 is 5~10cm being dried in 2~3 days in advance, cut the lateral bud later, and will
The lateral bud is placed in gnotobasis the processing that carries out disinfection；
3) it Fiber differentiation: by the Chinese cymbidium lateral bud after being disinfected in step 2, is inoculated in rhizomes induced medium and is trained
It supports；The induced medium are as follows: 1/2MS+6-BA 0.5~1.0mg/L+NAA 2.0~3.0mg/L+ active carbon 1g/L,
pH5.8；Condition of culture: 23~25 DEG C of temperature, 1000~1500 lux of light intensity, light application time is 16 h/d；
4) Multiplying culture: the rhizomes that step 3 Fiber differentiation goes out is cut into length 2~3cm/ item with aseptic operation knife, then is transferred
Into proliferated culture medium；The proliferated culture medium are as follows: MS+6-BA 1.0~3.0mg/L+NAA 0.3~0.5mg/L+ activity
Charcoal 1g/L+ banana puree 20g/L, pH5.8；Condition of culture: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is
16 h/d；
5) differentiation culture: the root that the rugosity of Multiplying culture is 0.32~0.38cm of φ in selecting step 4, length is 3.5~5.5cm
Shape stem, and be forwarded in differential medium, 9~11/bottle of inoculum density；The differential medium are as follows: MS+6-BA 1.0~
3.0mg/L+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 50g/L, pH5.8；Condition of culture: temperature 23~25
DEG C, 1500~1800 lux of light intensity, light application time is 16 h/d；
6) culture of rootage: the seedling that the specification broken up in step 5 is 3~5cm high is cut with aseptic operation knife and is forwarded to training of taking root
It supports in base, 10~12 seedlings of inoculum density/bottle；Meanwhile the differentiation culture rhizomes after seedling will be cut away be forwarded to step 5 again
In base；The root media are as follows: 1/2MS+NAA 0.3~0.5mg/L+ active carbon 1g/L+ banana puree 80g/L, pH5.8；Training
The condition of supporting: 23~25 DEG C of temperature, 1500~1800 lux of light intensity, light application time is 16 h/d；
7) acclimatization and transplants: when the test tube seedling of culture of rootage in step 6 it is long to 7~9cm high, root 3~5 when, which is put
The lower refining seedling in seeding room, hardening hot-house culture condition: 23~25 DEG C of temperature, 1800 ~ 2000 lux of light intensity, light application time 16
h/d；After hardening 5 days, take out tissue culture rooted seedling, reject and be attached to the culture medium of root, then transplant to air humidity 75%~85%,
It is grown in the greenhouse that 20~25 DEG C of temperature.
2. a kind of Chinese cymbidium method for tissue culture as described in claim 1, it is characterised in that: in step 1, the lateral bud is had
1. body trembles the old base in fundatrix strain root the following steps are included: select 2~triennial no disease and pests harm, after the Chinese cymbidium maternal plant that grows fine
Matter；2. by pine bark of the maternal plant plantation after being drained with the disinfection of 1% potassium permanganate: in perlite=4:1 mixed-matrix；Kind
After plant, the exposed 0.5~1cm of the reed of the maternal plant；3. the maternal plant planted is moved to indoor greenhouse growth, using alternation of wetting and drying method
Moisture is managed, moisture pouring is carried out in a manner of pouring root, spare when it is 5~10cm high that lateral bud, which grows to specification,；Indoor greenhouse training
The condition of supporting: temperature is 18~28 DEG C, 4500~6000lux of illumination, light application time 16h/d.
3. a kind of Chinese cymbidium method for tissue culture as described in claim 1, it is characterised in that: in step 2, the disinfection treatment is specific
Are as follows: the lateral bud cut is placed in 75% dehydrated alcohol and handles 1min, is during which transferred to super-clean bench rapidly；It will be by dehydrated alcohol 3
Lateral bud after reason sterilizes 10~12min with 0.1% mercuric chloride, abandons mercuric chloride, then the lateral bud is finally used with rinsed with sterile water 5~6 times
The filter paper sterilized blots the lateral bud surface moisture, spare.
4. a kind of Chinese cymbidium method for tissue culture as described in claim 1, it is characterised in that: in step 7, take out the tissue culture and take root
Seedling is planted in pine bark after rejecting is attached to the culture medium of root: perlite volume ratio is in the mixed-matrix of 4:1, in air
It is grown in 20~25 DEG C of humidity 75%~85%, temperature greenhouses.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116420621A (en) * | 2023-04-24 | 2023-07-14 | 玉林师范学院 | Method for promoting differentiation of cymbidium sinense rhizome buds |
| CN116439134A (en) * | 2023-04-24 | 2023-07-18 | 玉林师范学院 | Application of beta-farnesene in Mo Langen-shaped stem bud differentiation culture |
| CN116472962A (en) * | 2023-04-24 | 2023-07-25 | 玉林师范学院 | Application of lycopene in Mo Langen-like stem proliferation culture |
| CN116472961A (en) * | 2023-04-24 | 2023-07-25 | 玉林师范学院 | Application of squalene in Mo Langen-like stem subculture |
| CN116602208A (en) * | 2023-04-24 | 2023-08-18 | 玉林师范学院 | Method for regenerating cymbidium sinense rhizome plants |
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| CN116472961A (en) * | 2023-04-24 | 2023-07-25 | 玉林师范学院 | Application of squalene in Mo Langen-like stem subculture |
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Application publication date: 20191008 |