CN110283871A - A method of polysaccharide and rare saponin(e are produced using compound fungus fermented ginseng - Google Patents
A method of polysaccharide and rare saponin(e are produced using compound fungus fermented ginseng Download PDFInfo
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- CN110283871A CN110283871A CN201910617599.6A CN201910617599A CN110283871A CN 110283871 A CN110283871 A CN 110283871A CN 201910617599 A CN201910617599 A CN 201910617599A CN 110283871 A CN110283871 A CN 110283871A
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- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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Abstract
The invention discloses a kind of methods using compound fungus fermented ginseng production polysaccharide and rare saponin(e.It is using ginseng as primary raw material, with head mold, Mucor, aspergillus oryzae mixed bacteria and PDA culture medium solid state fermentation ginseng dry powder, then extracted separation panaxan and rare ginsenoside Rh1 and Rg3.This method has the characteristics that process conditions are controllable, raw material and product safety are edible, product yield is high.
Description
Technical field
The invention discloses produce polysaccharide and rare saponin(e using compound fungus fermented ginseng the invention discloses a kind of
Method.It is to be done using ginseng as primary raw material with head mold, Mucor, aspergillus oryzae mixed bacteria and PDA culture medium solid state fermentation ginseng
Powder, then extracted separation panaxan and rare ginsenoside Rh1 and Rg3.This method has controllable process conditions, raw material and production
The high feature of product safety edible, product yield.The invention belongs to food, drug manufacture field.
Background technique
Ginseng (Panax ginseng), Araliaceae.Mainly based on root, stem floral leaf can enter simultaneously for the part that it is used as medicine
Medicine.Currently, scholar analyzes about more than 300 kinds of ginseng effective component, most importantly ginsenoside and polysaccharide, there are also it is a small amount of its
His ingredient, including microelement, amino acid, organic acid, protein and vitamin etc..Wherein ginsenoside accounts for about 4% ~ 7 %, water
Soluble polysaccharide and alkaline polysaccharide account for 8. 3 % and the % of 7. 8 %~10. 0 respectively, and protein accounts for 5.34 %.
Ginsenoside is divided into dammarane type and oleanolic acid type by chemical structure difference.Wherein dammarane type includes ginseng two
Alcohol type and panaxatriol type.Currently, researcher, which separates out new saponin monomer, has had reached more than 50 kinds.Panoxadiol includes
Rc, Rd, Rb1, Rg3, Rb2, Rh2, Rb3 etc.；Panaxatriol type includes Rg1, Rh1 etc..They are mostly present in people
Join in root, a small amount of saponin(e is contained at other positions.
Ginsenoside plays adjustment effect to nerve, endocrine, immune, cardiovascular system, has anti-oxidant, antitumor effect
It answers.A few class monomers of panoxadiol type can play the role of calm, pacify.The Rg class of three alcohol type is to enable to nervous centralis
Excited substance.Multiclass ginsenoside can promote cortex hormone of aadrenaline to secrete, wherein the effect of diol type saponin(e Rb class
It is most obvious.Ginsenoside monomer Rb2 has very strong reducing blood sugar and blood fat function.With significant anti-tumor capacity in natural ginseng
The content of ginsenoside monomer Rh1, Rg3, Rh2 are considerably less.
Monomer Rh2 is the best monomer component of antitumor efficacy.Monomer Rg3 can be to a variety of inhibition of different system expressions
Tumour cell effect, and body itself does not have any nocuity.It can reach antitumor by enhancing the immunocompetence of body
Effect, directly inhibit tumour cell proliferative capacity, so that tumour is stopped growing.Experiments have shown that the primary chemical activity of ginseng
Ingredient is based on saponin(e and polysaccharide, when to ginseng assay, is usually subject to ginsenosides Rg1, Re and Rb1.By right
After ginseng is fermented, these three marker contents have different degrees of reduction, however are able to detect that rare saponin(e Rg3
0.76 0.52 mg/g of mg/g, Rh1, this may represent, and a part of ginsenoside Rg1, that Re, Rb1 pass through microbe conversion is adult
Join the substances such as rare saponin(e Rg3, Rh1.
Panaxan has good pharmacological activity, it is a kind of glucan substance.Tumour cell can not only be inhibited,
The blood glucose that body can also be reduced has a major impact in the immunology of body.
Mold fermentation can increase panaxan's output simultaneously and generate rare ginsenoside Rg3 and Rh1.Mold fermentation energy
Enough improve the yield 20%~30% of panaxan.It can be separated and be mentioned by microwave-assisted alcohol extracting method combination macroreticular resin after mold fermentation
The rare saponin(e of ginseng is taken, then panaxan is extracted by water extraction.
Summary of the invention
The object of the present invention is to provide a kind of methods using compound fungus fermented ginseng production polysaccharide and rare saponin(e.It should
Method can be improved the yield of panaxan, generate the rare saponin(e Rg3 and Rh1 of ginseng.The purpose of the present invention is pass through as follows
Step and method are realized:
A, the head mold of activation, Mucor, aspergillus oryzae PDA culture solution, which are inoculated on the ginseng pulverate of crushing, forms mould inoculation ginseng base
Matter；
B, mould inoculation ginseng matrix is placed in several days of normal temperature fermentation in culture kettle and obtains mold fermentation ginseng matrix；
C, the mold fermentation ginseng matrix drying after fermentation, crush, obtain for extracting panaxan and rare ginsenoside
Mold fermentation ginseng pulverate；
D, to mold fermentation ginseng pulverate, by microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction rare ginsenoside, then
Panaxan is extracted by water extraction and alcohol precipitation method.
Head mold, Mucor, the aspergillus oryzae PDA culture solution of the activation of step A, which are inoculated on the ginseng pulverate of crushing, to be formed mould and connects
Kind of ginseng matrix, head mold, Mucor, aspergillus oryzae ratio be 1:1:1, every strain ratio can float up and down 1~10 times；
The mould inoculation ginseng matrix of step B is placed in several days of normal temperature fermentation in culture kettle and obtains mold fermentation ginseng matrix,
The range of room temperature is 16 DEG C~42 DEG C, and several days ranges are 3d~6d；
Step D is to mold fermentation ginseng pulverate, by the rare saponin(e of microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction ginseng,
Its microwave-assisted power is 350w~450w, and the volume fraction of ethanol of alcohol extracting method is 50%~65%, the feed liquid of alcohol extracting method
Than for 1:65~1:75, rare ginsenoside is Rg3 and Rh1；
Step D is to mold fermentation ginseng pulverate, by microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction rare ginsenoside,
Panaxan, solid-liquid ratio 1:18~1:22, the extraction temperature 85 DEG C~90 of water extraction are extracted by water extraction and alcohol precipitation method again
DEG C, extraction time 100 min~120 min.
Specific embodiment
The following examples are intended to illustrate the invention, keeps it more clear, but is certainly not intended to limit the scope of the invention, embodiment
1:
1) head mold, Mucor, the aspergillus oryzae PDA culture solution activated respectively is 1:1:2 by its bacterium number density content, by inoculum concentration 1:1
It is inoculated on the ginseng pulverate of crushing and forms mould inoculation ginseng matrix；
2) mould inoculation ginseng matrix is placed in 26 DEG C of fermentation 5d in culture kettle and obtains mold fermentation ginseng matrix；
3) 75 DEG C of mold fermentation ginseng matrix drying after fermenting crush the sieving of 60 mesh, obtain for extracting panaxan and dilute
There is the mold fermentation ginseng pulverate of ginsenoside；
4) to mold fermentation ginseng pulverate, by the rare saponin(e of microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction ginseng,
Microwave-assisted power is 4000w, and the volume fraction of ethanol of alcohol extracting method is 55%%, and the solid-liquid ratio of alcohol extracting method is 1:70,
Macroreticular resin is D101 macroreticular resin；
5) to mold fermentation ginseng pulverate, after the rare saponin(e of microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction ginseng,
Remaining ginseng pulverate extracts panaxan, solid-liquid ratio 1:18~1:22, the extraction temperature of water extraction by water extraction and alcohol precipitation method again
85 DEG C~90 DEG C, the extraction time min of 100 min~120, it is useless which merges the rare saponin(e macroreticular resin separation of ginseng
Liquid obtains panaxan with 95%~99% ethyl alcohol alcohol deposition method.
Claims (4)
1. the object of the present invention is to provide a kind of method using compound fungus fermented ginseng production polysaccharide and rare saponin(e, the party
The purpose of method is realized by following step and method:
A, the head mold of activation, Mucor, aspergillus oryzae PDA culture solution, which are inoculated on the ginseng pulverate of crushing, forms mould inoculation ginseng base
Matter；
B, mould inoculation ginseng matrix is placed in several days of normal temperature fermentation in culture kettle and obtains mold fermentation ginseng matrix；
C, the mold fermentation ginseng matrix drying after fermentation, crush, obtain for extracting panaxan and rare ginsenoside
Mold fermentation ginseng pulverate；
D, to mold fermentation ginseng pulverate, by microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction rare ginsenoside, then
Panaxan is extracted by water extraction and alcohol precipitation method.
2. the head mold of the activation of A, Mucor, aspergillus oryzae PDA culture solution are inoculated on the ginseng pulverate of crushing and are formed according to claim 1
Mould is inoculated with ginseng matrix, head mold, Mucor, aspergillus oryzae ratio be 1:1:1, every strain ratio can float up and down 1~10 times.
3. the mould inoculation ginseng matrix of B is placed in several days of normal temperature fermentation in culture kettle and obtains mold fermentation according to claim 1
Ginseng matrix, the range of room temperature are 16 DEG C~42 DEG C, and several days ranges are 3d~6d.
4. D passes through microwave-assisted alcohol extracting method combination macroreticular resin separation and Extraction people to mold fermentation ginseng pulverate according to claim 1
Join rare saponin(e, microwave-assisted power is 350w~450w, and the volume fraction of ethanol of alcohol extracting method is 50%~65%, alcohol
The solid-liquid ratio of formulation is 1:65~1:75, and rare ginsenoside is Rg3 and Rh1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116731880A (en) * | 2023-06-16 | 2023-09-12 | 昆明理工大学 | Endophytic fungus Mucor multicastus and application thereof |
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