CN110272852A - A kind of Vibrio natriegen and its application - Google Patents

A kind of Vibrio natriegen and its application Download PDF

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CN110272852A
CN110272852A CN201910672205.7A CN201910672205A CN110272852A CN 110272852 A CN110272852 A CN 110272852A CN 201910672205 A CN201910672205 A CN 201910672205A CN 110272852 A CN110272852 A CN 110272852A
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vibrio
natriegens
natriegen
gene
vibrio natriegen
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CN110272852B (en
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江波
张涛
孟青
陈静静
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Shandong Haizhibao Seafood Co.,Ltd.
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Jiangnan University
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract

The invention discloses a kind of Vibrio natriegen and its applications, belong to microorganism field.Vibrio natriegen V.natriegens SK42.001 provided by the invention growth rapidly, carry natural plasmid and can expression alien gene, there is the Development volue and application potential as new ant algorithms biology.Meanwhile the bacterial strain contains the gene of the few algin catenase of the gene of coding algin catenase, coding, it may also be used for production prepares brown alga oligose Related product.

Description

A kind of Vibrio natriegen and its application
Technical field
The present invention relates to a kind of Vibrio natriegen and its applications, belong to microorganism field.
Background technique
Vibrio natriegen Vibrio natriegens is the most fast bacterium of the known speed of growth, when passing under certain condition Between less than 10 minutes, the speed of growth fastly by about one time than traditional mode organism Escherichia coli Escherichia coli, therefore no matter It is in scientific research or industrial production, it is that one kind has a high potential that the application of Vibrio natriegen, which will all greatly save time cost, It may replace the new ant algorithms biology of Escherichia coli.
The seminars such as Harvard University geneticist George Church delivered correlative study paper in 2016 in succession, referred to Superiority of the Vibrio natriegen compared to Escherichia coli and its feasibility as new ant algorithms biology out, and develop related gene Group edit tool, to promote host system building and the transformation of the bacterium to provide theoretical basis and technical support, and it is domestic current Have no relevant report.
Summary of the invention
The present invention screens one plant of rapid wild strain of growth from ooze, identified to be named as Vibrio natriegen Vibrio Natriegens SK42.001 is preserved in China typical culture collection center CCTCC, deposit number on January 5th, 2017 For CCTCC M2017011.
Vibrio natriegen Vibrio natriegens SK42.001 of the present invention has characteristics that
(1) growth is nearly twice of reference strain E. coli BL21 rapidly；It is 3% in NaCl concentration In LB culture medium, the culture Dai Shiwei 16.2min of Vibrio natriegen SK42.001, and E.coli BL21 is for Shi Zewei 31.4min； SK42.001 grows single colonie after the culture of LB plate streaking 5 hours, and E.coli BL21 then needs 10 hours；
(2) contain itself natural plasmid, size is about 3000bp；
(3) starch, maltose can be utilized with gelatin hydrolysate；
(4) aerobic growth；
(5) containing the base for encoding the gene (SEQ ID NO.2) of algin catenase, containing the few algin catenase of coding Because of (SEQ ID NO.3)；
(6) encoding gene of some albumen contained is different from other Vibrio natriegen strains, such as Xanthan lyase, pili Albumen TadC, TadE, CpaB etc..
Vibrio natriegen Vibrio natriegens SK42.001 of the present invention, can be dense in LB culture medium or NaCl It grows, or is being formulated in the LB culture medium that degree is 3% are as follows: sodium alginate 5g, (NH4)2SO4 5g、NaCl 30g、MgS04· 7H2O 1g、K2HPO4 2g、FeSO4·7H2O 0.01g, 1 000mL of distilled water are grown in the culture medium of pH 7.2.
Vibrio natriegen V.natriegens SK42.001 growth provided by the invention rapidly, carries natural plasmid and energy Enough expression alien genes, have the Development volue and application potential as new ant algorithms biology.Meanwhile the bacterial strain contain coding it is brown The gene of phycocolloid lyases, the gene for encoding few algin catenase, it may also be used for production prepares brown alga oligose Related product.
Biomaterial preservation
Vibrio natriegen Vibrio natriegens SK42.001 is preserved in Chinese Typical Representative culture on January 5th, 2017 Collection CCTCC, preservation address are the Wuhan Wuhan University, China, and deposit number is CCTCC NO:M2017011.
Detailed description of the invention
Fig. 1 bacterial strain SK42.001 microscope (1000X) observes photo,
The flat-plate bacterial colony form of Fig. 2 bacterial strain SK42.001,
Fig. 3 Electronic Speculum (20000X) observes photo,
The growth curve of Fig. 4 V.natriegens SK42.001 and E.coli BL21,
Fig. 5 recombinant plasmid pAWP89-GFP-Cm,
Fig. 6 SK42.001 engages recombinant bacterium and expresses GFP.
Fig. 7 M, DL5 000marker；The natural plasmid extracted in 1, SK42.001 wild mushroom, super spiral shell is presented in plasmid in figure Structure is revolved, band is in the position of actual size 1/2.
The Xanthan lyase gene of Fig. 8 Vibrio natriegen SK42.001 and the xanthan gum of V.natriegens CCUG16374 The comparison of lyase gene.
The bacterium of pili assembling the albumen TadC gene and V.natriegens CCUG16374 of Fig. 9 Vibrio natriegen SK42.001 The comparison of hair assembling albumen TadC gene.
The pili synthetic proteins TadE gene of Figure 10 Vibrio natriegen SK42.001 is with V.natriegens CCUG16374's The comparison of pili synthetic proteins TadE gene.
The pili Flp type assembling PROTEIN C paB gene and V.natriegens of Figure 11 Vibrio natriegen SK42.001 The comparison of the pili Flp type assembling PROTEIN C paB gene of CCUG16374.
Specific embodiment
Culture medium:
Fluid nutrient medium, screening fluid nutrient medium: sodium alginate 5g, (NH4)2SO4 5g、NaCl 30g、MgS04·7H2O 1g、K2HPO4 2g、FeSO4·7H2O 0.01g, distilled water 1 000mL, pH 7.2.
Plating medium: sodium alginate 5g, (NH4)2SO4 5g、NaCl 30g、MgS04·7H2O 1g、K2HPO4 2g、 FeSO4·7H2O 0.01g, distilled water 1 000mL, pH 7.2, agar 20g.
The screening technique of 1 Vibrio natriegen Vibrio natriegens of embodiment
(1) ooze is sampled near laminaria culture factory, Rongcheng City, Shandong Province, takes 1g sample in 50mL sterile water It is uniformly dispersed.
(2) 1mL supernatant is taken to be inoculated in 50mL screening fluid nutrient medium, 28 DEG C, 200rpm culture 2 days, dilution 10-6And It is coated on screening flat board culture medium, 28 DEG C are cultivated 2 days, and picking different shape single colonie obtains pure training through multiple plate streaking Support object.
(3) picking different shape single colonie, is inoculated in fluid nutrient medium, and 28 DEG C, 200rpm culture 2 days take supernatant Bacterial strain enzyme activity is measured, the higher bacterial strain of enzyme activity is chosen, entrusts China typical culture collection center preservation, and to the form of the bacterial strain Feature, physiological and biochemical property and 16S rDNA sequence are analyzed.
The identification of 2 Vibrio natriegen Vibrio natriegens of embodiment
(1) flat-plate bacterial colony form
As shown in Fig. 2, the flat-plate bacterial colony form of Vibrio natriegen SK42.001: it lines and is mushroomed out on plating medium, 28 DEG C of cultures grow single colonie afterwards for 24 hours, the rounded protrusion of bacterium colony, milky, it is wet slightly stick, surface is smooth, flush edge, diameter About 0.6~0.8cm.
(2) the thallus feature under Electronic Speculum
As shown in figure 3, thallus feature of the Vibrio natriegen SK42.001 under Electronic Speculum: thallus is short and small, and both ends blunt circle is bent to Arcuation, size are 1.2~1.4 μm of 0.6~0.8 μ m.
(3) physiological and biochemical property
The physiological and biochemical property of Vibrio natriegen SK42.001: as shown in Fig. 1, table 1, table 2, Vibrio natriegen is Gram's staining Feminine gender, aerobic growth, indole reaction is negative, and hydrolyzable gelatin and weakly hydrolyse aesculin cannot hydrolyze arginine, urea and β- Galactoside cannot utilize fructose, maltose, synanthrin, wood using glucose, sucrose, starch, arabinose, mannose Sugar, galactolipin, sorbose, xylitol.Specifically, Vibrio natriegen provided by the invention can with gelatin hydrolysate, can using starch, Maltose.
The 16S rDNA (SEQ ID NO.1) of Vibrio natriegen SK42.001 is compared with the data in ncbi database, The result shows that high with Vibrio natriegen homology.
1 bacterial strain SK42.001 physio-biochemical characteristics of table-enzyme activity, carbon source oxidation
+: positive reaction；: negative reaction
2 bacterial strain SK42.001 physio-biochemical characteristics of table-produce acid using carbon source
+: positive reaction；: negative reaction；W: weakly positive reaction
Feasibility of the 3 Vibrio natriegen Vibrio natriegens of embodiment as new ant algorithms biology
(1) speed of growth measures
Dai Shiwei 16.2min is cultivated in LB3 fluid nutrient medium (the LB culture medium that NaCl concentration is 3%), and referring to bacterium E.coli BL21 is for Shi Zewei 31.4min (Fig. 4) for strain；SK42.001 grows single bacterium after the culture of LB plate streaking 5 hours It falls, and E.coli BL21 then needs 10 hours.Illustrate SK42.001 growth rapidly, is approximately two times of E.coli.
(2) feasibility of SK42.001 expression alien gene
Broad host range plasmid pAWP89 is selected, building contains green fluorescent protein (GFP) reporter gene and chloramphenicol (Cm) resistance The recombinant plasmid pAWP89-GFP-Cm (Fig. 5) of genescreen label will by three parent's engagements using pRK2013 helper plasmid PAWP89-GFP-Cm imports SK42.001, and the SK42.001 recombinant bacterium after engagement issues green fluorescence (figure under blue light excitation 6).Illustrate that SK42.001 can be used as host strain expression external source target gene.
The above results show V.natriegens SK42.001 bacterial strain have as new ant algorithms biology Development volue with Application potential.
The genetic characteristics of 4 Vibrio natriegen Vibrio natriegens of embodiment
Vibrio natriegen SK42.001 and Daniel Gibson seminar, California Synthetic Genomics company it is used its In one plant of bacterial strain V.natriegens CCUG16374 consistency be 100%；Vibrio natriegen SK42.001 and Harvard University George Church seminar and common bacterial strain V.natriegens ATCC14048 (or DSM759) consistency of Gibson are 99%.But V.natriegens SK42.001 is not identical with V.natriegens CCUG16374 full-length genome.
(1) SK42.001 contains the encoding gene that some CCUG16374 do not have.Such as:
I SK42.001 contains the gene (SEQ ID NO.2) of coding algin catenase；
II SK42.001 contains the gene (SEQ ID NO.3) for encoding few algin catenase.
(2) SK42.001 and CCUG16374 have the encoding gene of some albumen different, such as:
I as shown in figure 8, the gene of SK42.001 coding Xanthan lyase and the coding xanthan gum of CCUG16374 crack The similitude of the gene of enzyme is 98%, there is 69 base differences, 18 Gap.
II some pilins related with bacterial pilli is constituted have larger difference
A. as shown in figure 9, pili assembling albumen TadC similitude is 77%, there are 201 base differences, 6 Gap.
B. as shown in Figure 10, pili synthetic proteins TadE similitude is 82%, there is 79 base differences, 6 Gap.
C. as shown in figure 11, it is 79% that pili Flp type, which assembles PROTEIN C paB similitude, there is 154 base differences, 10 Gap。
(3) V.natriegens SK42.001 itself is there are natural plasmid, and reported other V.natreigens plants There is no natural plasmids for system.
Picking V.natriegens SK42.001 single colonie is inoculated in 50mL fluid nutrient medium, 28 DEG C, 200rpm culture 2 days, extract the plasmid of SK42.001 wild strains using a small amount of extraction agent boxes of SanPrep pillar Plasmid DNA, and with 1% fine jade Lipolysaccharide gel electrophoresis detects (such as Fig. 7).It was found that SK42.001 wild strain contains itself natural plasmid, size is 3000bp or so. Superhelix is presented in plasmid in figure, and band is in the position of actual size 1/2.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention Enclosing subject to the definition of the claims.
SEQUENCE LISTING
<110>Southern Yangtze University
<120>a kind of Vibrio natriegen and its application
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 1401
<212> DNA
<213>vibrio marinopraesens SK42.001
<400> 1
caagtcgagc ggaaacgagt taactgaacc ttcgggggac gttaacggcg tcgagcggcg 60
gacgggtgag taatgcctag gaaattgccc tgatgtgggg gataaccatt ggaaacgatg 120
gctaataccg catgatgcct acgggccaaa gagggggacc ttcgggcctc tcgcgtcagg 180
atatgcctag gtgggattag ctagttggtg aggtaagggc tcaccaaggc gacgatccct 240
agctggtctg agaggatgat cagccacact ggaactgaga cacggtccag actcctacgg 300
gaggcagcag tggggaatat tgcacaatgg gcgcaagcct gatgcagcca tgccgcgtgt 360
gtgaagaagg ccttcgggtt gtaaagcact ttcagtcgtg aggaaggttt atgcgttaat 420
agcgtataga tttgacgtta gcgacagaag aagcaccggc taactccgtg ccagcagccg 480
cggtaatacg gagggtgcga gcgttaatcg gaattactgg gcgtaaagcg catgcaggtg 540
gtttgttaag tcagatgtga aagcccgggg ctcaacctcg gaatagcatt tgaaactggc 600
agactagagt actgtagagg ggggtagaat ttcaggtgta gcggtgaaat gcgtagagat 660
ctgaaggaat accggtggcg aaggcggccc cctggacaga tactgacact cagatgcgaa 720
agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgtctact 780
tggaggttgt ggccttgagc cgtggctttc ggagctaacg cgttaagtag accgcctggg 840
gagtacggtc gcaagattaa aactcaaatg aattgacggg ggcccgcaca agcggtggag 900
catgtggttt aattcgatgc aacgcgaaga accttaccta ctcttgacat ccagagaact 960
ttccagagat ggattggtgc cttcgggaac tctgagacag gtgctgcatg gctgtcgtca 1020
gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tccttgtttg 1080
ccagcgagta atgtcgggaa ctccagggag actgccggtg ataaaccgga ggaaggtggg 1140
gacgacgtca agtcatcatg gcccttacga gtagggctac acacgtgcta caatggcgca 1200
tacagagggc ggccaacttg cgaaagtgag cgaatcccaa aaagtgcgtc gtagtccgga 1260
ttggagtctg caactcgact ccatgaagtc ggaatcgcta gtaatcgtgg atcagaatgc 1320
cacggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccatgg gagtgggctg 1380
caaaagaagt aggtagttta a 1401
<210> 2
<211> 1566
<212> DNA
<213>vibrio marinopraesens SK42.001
<400> 2
atgaagcata ttttcttcaa aagcttgtta gcttcttcaa tcctattggc tgttggttgt 60
aacagcactg caactgcgaa ggctgatttc ccaaacaatc aagaaaccgg cgttgacatt 120
ctaactcctg ttgcaatcac ggcgagtagc catgatggta atgtgcctga gaacttactt 180
gaccaagata ttatgactcg ctgggcagcg aacggtgacg gtgagtgggc aatgttggat 240
tacggctcag tttatgggtt cgatgcaatc caagcgtcgt ttagtaaagg taatgaacgt 300
gtcacgtcat ttgatgttca gttcagcaca gatggtgaaa actgggtaac ggttattgaa 360
ggtgcacaaa gctctggtcg tgctcttggt ctggaacgct tccagttcga gcctgcggta 420
aaagctcgtt atgtacgtta cgttggccac ggcaatacca aaaaccaatg gaacgctgtt 480
actgaaatgg ccgcggttaa ctgtggaatc aatgcgtgcc cggcaagcca tgtcattacc 540
gatgatgttg ttaaagctga agcgactatg attgctgcaa tgaaggctaa ggaaaaagcg 600
caaaaggaac tccttaaaaa taatcgcaaa ggtgatttcg gagaaccaat cgtccgtcct 660
tgcgggacga cagtgacgtg tgacctaact aaagcaatgc catccccaac gctaccggct 720
gttccactag ctaagaatgc accaggccaa aactttgacc tgacgcgctg gaaactgaca 780
acgcctttcg atcacgacaa agacggccgc gctgatgata ttgatgagtg ggatatggca 840
aacggcttcc agcacccaga tatcttctac acagctgatg atggcggcat ggttttcaag 900
agctatgtaa aaggtgcacg tacctctaaa aatactaagt acgcacgtac agagttgcgc 960
actatgctgc gtgcgggtga gaagtctcac agtacaaaag gtgtaaatcc aaataactgg 1020
gtattcagct cagcgccggt agaagatcag aaagcagcgg gtggggtaga tggcacgctt 1080
gaggcaactc tgaagattga ccatgcaacc acaacgggtc agtcacacga agttggccgt 1140
ttcattatcg gtcagattca tgacaaagat gatgagccaa ttcgccttta ctaccgtaag 1200
ctaccagacc agccaacagg tacggtttac ttcgctcacg aaaaaaccaa aacaggtact 1260
gaagattact acagcctggt tggtgatatg actggtgaaa tcggtaacga tggtatcgcg 1320
ctaggtgaaa aattcagcta catcattgat gtaaaaggca acacgatgac agttacggta 1380
aaacgtgacg gtaaagatga tgttgtacaa gtcgtagata tgagtgacag tggttatgat 1440
gagggtggcc gatacatgta cttcaaggcc ggtgtttata accagaatat gtacggcaat 1500
ccagatgatt acgctcaagc aactttctac aagctagatc aatcttttgg taagtaccaa 1560
ggctag 1566
<210> 3
<211> 2082
<212> DNA
<213>vibrio marinopraesens SK42.001
<400> 3
atgagcaacc aaaagtctct tgacgctatt agaaagataa agctggagaa tgatacatca 60
gctggaaacc ttgttgactt attgcctatc gaagtacaaa aacgcgactt cgatctgtca 120
tttctagata ccttgagtga agctcgtcca cgcctgctag tccaagctga gcagcttgaa 180
gaattcaaag cgaaagtaca ggctgatgag tcttactgta tgttcgacga tttctacaat 240
aactcgacgg ttaagtttct tgaaactgaa ccttatgaag agccgaagcc ttacccggaa 300
gagacagttg gaaaagcttc tctatggcgt ccttactggc gtcaaatgta tgttgattgc 360
cagatggcgc ttaacgcgac acgtaacttg gctattgccg gaatcgttaa agaagatgaa 420
gcgctgattg ctaaagccaa agcatggact ctgaagctgt cgacttatga tcctgaaggc 480
gtcacgtctc gtggttacaa tgacgaagct gcgtttcgtg ttattgcggc tatggcatgg 540
ggttacgatt ggttacatgc ttactttacc gatgaagagc ggcagcaagt tcaaaatgca 600
ttagtgaccc gcctggacga aatcatgcac cacctgaaag tgacggttga cctacttagc 660
aacccgctga acagtcacgg tgttcgttct atttcttcag ctatcatccc aacatgtatc 720
gcgctttatc acgatcaccc gaaagcgggt gagtacatcg catacgcgtt agaatattat 780
gcagtgcact acccaccatg gggcggagaa gatggcggct gggcagaagg tcctgattac 840
tggaatacgc aaacggcttt cctaggtgaa gctttcgacc tattaaaagc gtactgtggc 900
gtagacatgt tcaataaaac gttctacgaa aacaccggcg atttcccact atactgtatg 960
cccgttcact ctaagcgtgc gagtttctgt gaccagtcat cgatcggtga tttccctggt 1020
ctgaagctgg cttacaatat caagcattac gcaggtgtta accagaagcc agagtacgtt 1080
tggtattaca atcaactaaa aggtcgtgat acagaagctc acactaaatt ctacaactat 1140
ggctggtggg attttggcta cgacgatctt cgtttcaaca ttctttggga tgcgcctgag 1200
gagcaagcgc cgtcaaatga cccgttgttg aaagtattcc cgattacagg ttgggctgca 1260
ttccacaata agatgactga acgtgataac cacatccata tggtgttcaa gtgctctccg 1320
tttggttcta tcagtcattc acatggtgac cagaacgcat ttacgctaca tgccttcggt 1380
gaaactttag cggcgatcac cggctattac ggcggcttcg gcgtagatat gcacacgaaa 1440
tggcgtcgcc aaacgttctc taaaaacttg cctctatttg gtggtaaagg ccaatatggc 1500
gagaacaaaa atacaggcta cgaaaaccac caagaccgtt tctgtattga agcgggcggt 1560
aatatcacag actacgatac tgaatctgat gtgaagatgg tagaaggtga tgcaactgca 1620
tcttataagt acttcgttcc tgagatcgaa tcttacaagc gtaagatctg gtttgttcaa 1680
ggcaaagttt ttgtaatgca agacaaggcg acgctttctg aagagaaaga catgacgtgg 1740
ttgatgcata ccacgtttgc taacgaagtg gcggataagt cattcactgt ccggggtgaa 1800
gtcgcgcacc tagacgtgaa ctttattaac gaatccgctg gtaatatcgc ttctgttaag 1860
aacgttgaag gcttcggtga agttgatccg tatgaataca aagagttaga aatccaccgt 1920
catgttgaag tggaattcaa gccatccaac gagcacaaca tcttaactct tctggttcct 1980
aataagaacg agggtgagca agttgaagtt tcttataagc tggaaggtaa cgtcttactt 2040
ctgacagtag atggtgaagc cgtagaaatt gatttgtctt aa 2082

Claims (9)

1. Vibrio natriegen (Vibrio natriegens) SK42.001 is preserved in Chinese Typical Representative culture on January 5th, 2017 Collection CCTCC, preservation address are the Wuhan Wuhan University, China, and deposit number is CCTCC NO:M2017011.
2. a kind of algin catenase, which is characterized in that from Vibrio natriegen (Vibrio described in claim 1 natriegens)SK42.001。
3. a kind of algin catenase according to claim 2, which is characterized in that encode the nucleotides sequence of the gene of the enzyme Column are as shown in SEQ ID NO.2.
4. a kind of widow's algin catenase, which is characterized in that from Vibrio natriegen (Vibrio described in claim 1 natriegens)SK42.001。
5. a kind of few algin catenase according to claim 4, which is characterized in that encode the nucleotide of the gene of the enzyme Sequence is as shown in SEQ ID NO.3.
6. (Vibrio natriegens) SK42.001 of Vibrio natriegen described in claim 1 is preparing the application in brown alga oligose.
7. Vibrio natriegen described in claim 1 (Vibrio natriegens) SK42.001 is as the application in type strain.
8. the method for cultivating Vibrio natriegen (Vibrio natriegens) SK42.001 described in claim 1, which is characterized in that Oxygen is provided, using sodium alginate, gelatin, starch, maltose or glucose as carbon source.
9. (Vibrio natriegens) SK42.001 of Vibrio natriegen described in claim 1 answering in building genetic engineering bacterium With.
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Publication number Priority date Publication date Assignee Title
WO2021012959A1 (en) * 2019-07-24 2021-01-28 江南大学 Alginate lyase and application thereof
US11993795B2 (en) 2019-07-24 2024-05-28 Jiangnan University Alginate lyase and application thereof
CN111500484A (en) * 2020-02-14 2020-08-07 上海交通大学 Three newly separated vibrio capable of growing quickly and its application
CN111500484B (en) * 2020-02-14 2022-07-26 上海交通大学 Fast growing vibrio and its application
CN111197065A (en) * 2020-02-24 2020-05-26 江南大学 Method for producing algin hydrolysate
CN115838677A (en) * 2022-03-29 2023-03-24 哈尔滨工业大学（深圳） Genetically engineered vibrio natriegens, preparation method thereof and application thereof in heavy metal treatment
CN115838677B (en) * 2022-03-29 2023-12-05 哈尔滨工业大学（深圳） Genetically engineered vibrio natrii and preparation method thereof, and application thereof in heavy metal treatment
CN115960802A (en) * 2022-09-29 2023-04-14 浙江大学杭州国际科创中心 Engineering bacterium of vibrio natriegens for high yield recombination protein and its application

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