CN110272831A - A kind of Cordceps militaris bacterial strain and its method for producing hypha powder - Google Patents
A kind of Cordceps militaris bacterial strain and its method for producing hypha powder Download PDFInfo
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- CN110272831A CN110272831A CN201910699832.XA CN201910699832A CN110272831A CN 110272831 A CN110272831 A CN 110272831A CN 201910699832 A CN201910699832 A CN 201910699832A CN 110272831 A CN110272831 A CN 110272831A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H15/00—Fungi; Lichens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
The invention discloses a kind of Cordceps militaris bacterial strain, which is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number are as follows: CGMCC No.17799.The present invention is grown on potato culture using isolated one plant new Cordceps militaris bacterial strain, obtains the Chinese caterpillar fungus hypha with high nutritive value.The mannitol content of the Chinese caterpillar fungus strain grown in potato culture reaches 12.31 ± 0.22mg/g, and cordycepin content reaches 29.22 ± 1.11 μ g/g, the content being significantly higher than in existing Cordceps militaris strain fermentation mycelia powder.
Description
Technical field
The invention belongs to medicine and health-care food technical fields, and in particular to a kind of Cordceps militaris bacterial strain and its production mycelia
The method of powder.
Background technique
Cordceps militaris is Ascomycota, meat seat mesh, Clavicipitaceae, the type sepecies of Cordyceps.Scientific name is Cordyceps
Militaris also known as Cordyceps militaris.The worldwide distribution of Cordceps militaris, natural resources quantity are seldom.Nineteen fifty, Germany scientist
Cunningham observes not perishable by the insect tissue of Cordceps militaris parasitism, and then therefrom isolates a kind of antibacterial material,
3'-Deoxyadenosine is named as cordycepin.Cordceps militaris also contains cordycepic acid, and Chinese medicine thinks, cordycepic acid enters lung kidney two warp, can mend
Lung yin, and energy kidney-replenishing, cure mainly kidney deficiency, and impotence and seminal emission, soreness of waist and knee joint, eak after being ill, chronic cough is weak, phthisical cough phlegm blood, and spontaneous perspiration is stolen
Sweat etc. is a kind of unique Chinese medicine that can balance, adjust yin-yang simultaneously.
Cordceps militaris is grown on mesophorbium, coryphile naturally, requires harshness to growing environment, excavation is not easy, causes after excavation to environment
Irreversible destruction is a large amount of blindly unreasonable in recent years again since the main place of production ecological environment of Cordceps militaris is by artificial serious destruction
Excavation causes resource to reduce increasingly, and yield declines year by year, and protection utilizes Cordceps militaris resource with reasonable, seeks by artificially developing energy
Produce the Cordceps militaris bacterial strain of hypha powder, with meet ever-increasing health care and it is medicinal the needs of, have become extremely urgent
Business.But although the Cordceps militaris of artificial culture can survive and generate hypha powder at present, nutritive value is very low.
Summary of the invention
The purpose of the present invention is to provide a kind of Cordceps militaris bacterial strain and its methods for producing hypha powder.
A kind of Cordceps militaris (Cordyceps militaris) bacterial strain, the Cordceps militaris bacterial strain are preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, culture presevation number are as follows: CGMCC No.17799；Strain name is Cordyceps militaris
Co53 2019YZ513, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica.
A kind of preparation method of scarlet caterpiller fungus mycelia carries out in accordance with the following steps:
(1) Cordceps militaris strain is taken in cryovial, is inoculated with into solid medium, is cultivated 5-8 days at lower 22 DEG C of dark condition, it is described
Cordceps militaris culture presevation number are as follows: CGMCC No.17799；
(2) it is seeded to fluid nutrient medium, 22 DEG C obtain cordyceps sinensis bacterium solution in fermented and cultured 8-12 days；
(3) the cordyceps sinensis bacterium solution in step (2) is inoculated into potato culture, under the conditions of 25 DEG C, illumination cultivation 6-10 for 24 hours
It, obtains mycelium, and mycelium is filtered, obtains scarlet caterpiller fungus mycelia powder after dries pulverizing.
The solid medium is by glucose: yeast powder: peptone: dehydrated potato powder 1:1:1:(2-8) weight ratio
It prepares, strain inoculum concentration is 1-3%.
The fluid nutrient medium contains glucose 3%, and yeast powder 1%, peptone 1%, strain inoculum concentration is 6-12%.
The strain inoculum concentration of step (3) is 3-8%.
The potato culture removes bud eye the preparation method comprises the following steps: potato is cleaned, and is cut into small pieces, 3-5 is added
The water of times weight, boils 15-25 min, the foam of generation is cleared up in boiling part, with filtered through gauze after having boiled, is added in filtrate
Glucose 5%, yeast powder 2%, peptone 2%.
Step (1) described solid medium, step (2) fluid nutrient medium and step (3) described potato culture
Pass through high pressure sterilization before inoculation, sterilizing parameter is 121 DEG C, 0.11MPa-0.13 MPa, 30min.
Beneficial effects of the present invention: the present invention utilizes isolated one plant new Cordceps militaris bacterial strain (CGMCC No.17799)
It is grown on potato culture, obtains the Chinese caterpillar fungus hypha with high nutritive value.Growth obtains in potato culture
The mannitol content of Chinese caterpillar fungus strain reaches 12.31 ± 0.22mg/g, and cordycepin content reaches 29.22 ± 1.11 μ g/g, significantly high
Content in existing Cordceps militaris strain fermentation mycelia powder.
Specific embodiment
The present invention will be further described combined with specific embodiments below.
The preservation of Cordceps militaris bacterial strain of the present invention illustrates: strain name is Cordceps militaris Co53 2019YZ513, the bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on June 14th, 2019, culture presevation is compiled
Number are as follows: CGMCC No.17799；Preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism are ground
Study carefully institute.
Solid medium described in following embodiments, fluid nutrient medium and potato culture go out before inoculation by high pressure
Bacterium, sterilizing parameter are 121 DEG C, 0.12 MPa, 30min.
The separation of 1 Cordceps militaris bacterium of embodiment
Immature fresh Cordceps militaris is acquired using tissue isolation.It cleans, is repeatedly rinsed with sterile water, be put into 0.1% mercuric chloride
It is sterilized 8 minutes in solution, washes away mercuric chloride with sterile purified water.Aseptically, crust is cut, enteron aisle is avoided, is cut fair and clear
Tissue, shreds, is connected to and is separately cultured primary surface, as cultivating under the conditions of 20 DEG C of temperature.The isolation medium that the above method uses
Formula be SDB fluid nutrient medium.The SDB fluid nutrient medium includes glucose 5%, yeast powder 1%, peptone 1%.
The identification of 2 Cordceps militaris bacterium of embodiment
Strain is inoculated on solid medium and is cultivated, on solid medium, bacterium colony is white, and bacterium colony surface is not raised, has
One layer of fine and close villiform mycelium, there is gauffer, and the culture medium back side is in apparent sepia.The colourless width of its mycelium of microexamination
2.8–5.2μm.Conidium is colourless, no diaphragm, kidney shape or oblong, Dan Sheng or 3-8 a group, is an oval mucous membrane
It is surrounded.
The preparation of 3 scarlet caterpiller fungus mycelia of embodiment
The preparation method of scarlet caterpiller fungus mycelia carries out in accordance with the following steps:
(1) Cordceps militaris strain is taken in cryovial, is inoculated with into solid medium, is cultivated 7 days at lower 22 DEG C of dark condition, the north
Cordyceps species deposit number are as follows: CGMCC No.17799；The solid medium presses glucose: yeast powder: peptone: potato
The weight ratio that powder is 1:1:1:5 is prepared, and strain inoculum concentration is 2%；
(2) it is seeded to fluid nutrient medium, 22 DEG C obtain cordyceps sinensis bacterium solution in fermented and cultured 10 days；The fluid nutrient medium contains glucose 3%,
Yeast powder 1%, peptone 1%, strain inoculum concentration are 8%；
(3) the cordyceps sinensis bacterium solution in step (2) is inoculated into potato culture, strain inoculum concentration is 5%；Under the conditions of 25 DEG C,
Illumination cultivation 8 days for 24 hours, obtain mycelium, and mycelium is filtered, obtains scarlet caterpiller fungus mycelia powder after dries pulverizing；The potato training
That supports base removes bud eye the preparation method comprises the following steps: potato is cleaned, and is cut into small pieces, the water of 4 times of weight is added, boils 20min, boils
The foam that cleaning generates during boiling has boiled rear filtered through gauze, glucose 5%, yeast powder 2%, peptone 2% is added in filtrate.
The preparation of 4 scarlet caterpiller fungus mycelia of embodiment
The preparation method of scarlet caterpiller fungus mycelia carries out in accordance with the following steps:
(1) Cordceps militaris strain is taken in cryovial, is inoculated with into solid medium, is cultivated 5 days at lower 22 DEG C of dark condition, the north
Cordyceps species deposit number are as follows: CGMCC No.17799；The solid medium presses glucose: yeast powder: peptone: potato
The weight ratio that powder is 1:1:1:7 is prepared, and strain inoculum concentration is 3%；
(2) it is seeded to fluid nutrient medium, 22 DEG C obtain cordyceps sinensis bacterium solution in fermented and cultured 8 days；The fluid nutrient medium contains glucose 3%, ferment
Female powder 1%, peptone 1%, strain inoculum concentration are 12%；
(3) the cordyceps sinensis bacterium solution in step (2) is inoculated into potato culture, strain inoculum concentration is 8%；Under the conditions of 25 DEG C,
Illumination cultivation 6 days for 24 hours, obtain mycelium, and mycelium is filtered, obtains scarlet caterpiller fungus mycelia powder after dries pulverizing；The potato training
That supports base removes bud eye the preparation method comprises the following steps: potato is cleaned, and is cut into small pieces, the water of 3 times of weight is added, boil 15 min,
The foam that generation is cleared up in boiling part uses filtered through gauze, glucose 5%, yeast powder 2%, peptone is added in filtrate after having boiled
2%。
The preparation of 5 scarlet caterpiller fungus mycelia of embodiment
The preparation method of scarlet caterpiller fungus mycelia carries out in accordance with the following steps:
(1) Cordceps militaris strain is taken in cryovial, is inoculated with into solid medium, is cultivated 8 days at lower 22 DEG C of dark condition, the north
Cordyceps species deposit number are as follows: CGMCC No.17799；The solid medium presses glucose: yeast powder: peptone: potato
The weight ratio that powder is 1:1:1:3 is prepared, and strain inoculum concentration is 1%；
(2) it is seeded to fluid nutrient medium, 22 DEG C obtain cordyceps sinensis bacterium solution in fermented and cultured 12 days；The fluid nutrient medium contains glucose 3%,
Yeast powder 1%, peptone 1%, strain inoculum concentration are 6%；
(3) the cordyceps sinensis bacterium solution in step (2) is inoculated into potato culture, strain inoculum concentration is 4%；Under the conditions of 25 DEG C,
Illumination cultivation 10 days for 24 hours, obtain mycelium, and mycelium is filtered, obtains scarlet caterpiller fungus mycelia powder after dries pulverizing；The potato training
That supports base removes bud eye the preparation method comprises the following steps: potato is cleaned, and is cut into small pieces, the water of 5 times of weight is added, boil 25 min,
The foam that generation is cleared up in boiling part uses filtered through gauze, glucose 5%, yeast powder 2%, peptone is added in filtrate after having boiled
2%。
Experimental example 1: the measurement of mannitol in cordyceps mycelia
The mannitol of 1g is weighed in large beaker, the mannitol solution that 1L deionized water is configured to 1mg/mL is measured, takes 7 examinations
It is 1-7 that pipe is numbered respectively, and the 1mg/mL of 0mL, 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL are added thereto
Mannitol solution, add deionized water, making the mannitol concentration in each test tube is respectively 0 μ g/mL, 10 μ g/mL, 20 μ
g/mL,30μg/mL,40μg/mL,50μg/mL,60μg/mL.Take 1mL to new test tube, test tube respectively the solution that configuration obtains
Correspondence markings 1-7.1mL sodium periodate solution is added into each test tube again, is placed at room temperature for 10min.Add thereto after 10min
Enter 2mL0.01%L- rhamonic acid, is eventually adding the Nash reagent of 1mL, 53 DEG C of heating water bath 15min.
Experiment measures the standard curve of mannitol content, regression equation y=68.882x+0.0398, R2=
0.9919。
Culture medium of the Example 4-5 containing scarlet caterpiller fungus mycelia, is placed in the culture dish of dry sterilization, is placed on 60 DEG C of baking
It is dried overnight in case.Scarlet caterpiller fungus mycelia 0.5g after weighing drying is placed in ultrasound 15min, centrifuging and taking in the centrifuge tube of 50mL
Supernatant takes supernatant to be placed in the centrifuge tube of 5mL after being centrifuged again, and it is stand-by that addition deionized water is settled to 5mL i.e. 0.1g/mL.
7 test tubes are taken, are added the 0.1g/mL's of 0mL, 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL thereto
Cordyceps sinensis liquid, adds deionized water, and making the cordyceps sinensis liquid concentration in each test tube is respectively 0mg/mL, 1mg/mL, 2mg/mL, 3mg/
mL,4mg/mL,5mg/mL,6mg/mL.Take 1mL to new test tube, test tube correspondence markings 1-7 respectively the solution that configuration obtains.
1mL sodium periodate solution is added into each test tube again, is placed at room temperature for 10min.2mL 0.01%L- mouse is added after 10min thereto
Lee's acid, is eventually adding the Nash reagent of 1mL, 53 DEG C of heating water bath 15min.
The OD value measured in the sample for the Cordyceps Militaris that experiment obtains is brought into regression equation, the experiment of embodiment 4 is measured
The content of group mannitol is 12.31 ± 0.22mg/g, and the content of 5 experimental group mannitol of embodiment is 11.27 ± 0.38mg/g.
Experimental example 2: the measurement of cordycepin in cordyceps mycelia
Using liquid chromatography carry out cordycepin measurement, chromatographic column be ODS-3 (4.6mm × 250mm, 5 μm), 30 DEG C of column temperature.
Detector is diode array UV detector, Detection wavelength 260nm.Mobile phase acetonitrile: water=80:20, flow velocity 0.8ml/
min。
The content for measuring 4 experimental group cordycepin of embodiment is 29.22 ± 1.11 μ g/g, and embodiment 5 tests containing for cordycepin
Amount is the general content of cordycepin in 28.21 ± 2.24 natural scarlet caterpiller fungus mycelias of μ g/g(in 21 μ g/g).
Claims (7)
1. a kind of Cordceps militaris bacterial strain, which is characterized in that the Cordceps militaris bacterial strain is preserved in Chinese microorganism strain preservation management committee
Member's meeting common micro-organisms center, culture presevation number are as follows: CGMCC No.17799.
2. a kind of preparation method of scarlet caterpiller fungus mycelia, which is characterized in that carry out in accordance with the following steps:
(1) Cordceps militaris strain is taken in cryovial, is inoculated with into solid medium, is cultivated 5-8 days at lower 22 DEG C of dark condition, it is described
Cordceps militaris culture presevation number are as follows: CGMCC No.17799；
(2) it is seeded to fluid nutrient medium, 22 DEG C obtain cordyceps sinensis bacterium solution in fermented and cultured 8-12 days；
(3) the cordyceps sinensis bacterium solution in step (2) is inoculated into potato culture, under the conditions of 25 DEG C, illumination cultivation 6-10 for 24 hours
It, obtains mycelium, and mycelium is filtered, obtains scarlet caterpiller fungus mycelia powder after dries pulverizing.
3. the preparation method of scarlet caterpiller fungus mycelia according to claim 2, which is characterized in that the solid medium presses grape
Sugar: yeast powder: peptone: dehydrated potato powder 1:1:1:(2-8) weight ratio prepare, strain inoculum concentration be 1-3%.
4. the preparation method of scarlet caterpiller fungus mycelia according to claim 2, which is characterized in that the fluid nutrient medium contains grape
Sugar 3%, yeast powder 1%, peptone 1%, strain inoculum concentration are 6-12%.
5. the preparation method of scarlet caterpiller fungus mycelia according to claim 2, which is characterized in that the strain inoculum concentration of step (3)
For 3-8%.
6. the preparation method of scarlet caterpiller fungus mycelia according to claim 2, which is characterized in that the system of the potato culture
Preparation Method are as follows: potato is cleaned and removes bud eye, is cut into small pieces, the water of 3-5 times of weight is added, boils 15-25 min, boils
The foam for clearing up generation in the process uses filtered through gauze, glucose 5%, yeast powder 2%, peptone 2% is added in filtrate after having boiled.
7. the preparation method of scarlet caterpiller fungus mycelia according to claim 2, which is characterized in that step (1) described solid culture
Base, step (2) fluid nutrient medium and step (3) described potato culture pass through high pressure sterilization, sterilizing ginseng before inoculation
Number is 121 DEG C, 0.11MPa-0.13 MPa, 30min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115136848A (en) * | 2022-07-25 | 2022-10-04 | 什邡昊阳农业发展有限公司 | Production process of cordyceps militaris solid-state hypha powder |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531968A (en) * | 2008-05-04 | 2009-09-16 | 贵州大学 | Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation |
| WO2013155864A1 (en) * | 2012-04-16 | 2013-10-24 | 济南亿安生物研究所 | Method for producing cordycepic acid by means of liquid fermentation of cordyceps militaris |
| CN108410744A (en) * | 2018-03-21 | 2018-08-17 | 福建师范大学 | A kind of fusant bacterial strain producing polysaccharide, adenosine and cordycepin |
| CN108624510A (en) * | 2017-03-22 | 2018-10-09 | 中国科学院微生物研究所 | Cordyceps militaris single cross distribution type bacterial strain 2461 and its application |
-
2019
- 2019-07-31 CN CN201910699832.XA patent/CN110272831A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531968A (en) * | 2008-05-04 | 2009-09-16 | 贵州大学 | Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation |
| WO2013155864A1 (en) * | 2012-04-16 | 2013-10-24 | 济南亿安生物研究所 | Method for producing cordycepic acid by means of liquid fermentation of cordyceps militaris |
| CN108624510A (en) * | 2017-03-22 | 2018-10-09 | 中国科学院微生物研究所 | Cordyceps militaris single cross distribution type bacterial strain 2461 and its application |
| CN108410744A (en) * | 2018-03-21 | 2018-08-17 | 福建师范大学 | A kind of fusant bacterial strain producing polysaccharide, adenosine and cordycepin |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115136848A (en) * | 2022-07-25 | 2022-10-04 | 什邡昊阳农业发展有限公司 | Production process of cordyceps militaris solid-state hypha powder |
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