CN1102620C - 松香包埋剂及制作方法 - Google Patents
松香包埋剂及制作方法 Download PDFInfo
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- CN1102620C CN1102620C CN98125709A CN98125709A CN1102620C CN 1102620 C CN1102620 C CN 1102620C CN 98125709 A CN98125709 A CN 98125709A CN 98125709 A CN98125709 A CN 98125709A CN 1102620 C CN1102620 C CN 1102620C
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- rosin
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 10
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 claims abstract description 7
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- SWXVUIWOUIDPGS-UHFFFAOYSA-N diacetone alcohol Natural products CC(=O)CC(C)(C)O SWXVUIWOUIDPGS-UHFFFAOYSA-N 0.000 abstract 1
- 238000002844 melting Methods 0.000 abstract 1
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- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 2
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Abstract
本发明属于生物材料切片的包埋剂及制作方法。目前，世界上还没有切实可行的组织包埋技术适用于昆虫体壁(含外骨骼)的连续切片。本发明针对昆虫体壁的特质性，采用优质松香、石蜡为原料，加入少量丙酮、叔丁醇，其配比由切片时室温、材料硬度、大小而定。本发明广泛适用于各种生物材料的软、硬组织。具有支持强度高、韧性好、溶点低、切片不受室温限制、操作简便、易于推广、价格低廉等优点。
Description
本发明属于生物材料切片的包埋剂。
目前，世界上常用的生物材料切片所用的包埋剂有：石蜡、火棉胶、炭蜡等，包括超薄切片用的环氧树脂及冰冻切片技术等，都不适于昆虫体壁组织(含外骨骼)的连续切片。由于昆虫的外骨骼坚硬、性脆、易碎，采用以上任何一种包埋剂，都不能有效地对昆虫体壁的外骨骼进行连续切片观察。如石蜡，即便是溶点高的(如58℃～62℃)，也支持不住成虫发达的外骨骼，而且渗透温度较高(65℃左右)，对昆虫材料有较强的破坏作用；环氧树脂，也只是对那些骨化程度不高，微小的器官有效。由于没有一种适合于昆虫体壁组织(含外骨骼)连续切片的包埋剂，长期以来，有关昆虫体壁组织显微结构的研究，很少有人问津。
为了解决昆虫体壁组织(含外骨骼)连续切片这一世界性的难题，特研制本发明，试制出目前对昆虫体壁组织特效的包埋剂。
本剂主要是由松香(Colophony)及石蜡(Paraffin)加其它辅剂配制而成。确切的配比，要随切片时的室温，生物材料的硬度、大小而定。我们试制出六个型号的包埋剂适于在18～36℃条件下进行连续切片。
该剂的优点：①支持强度高，对各种成虫的外骨骼均有效；②韧性好，切出的带弹性好，明显优于石蜡(在同等条件下，石蜡性脆，蜡带易断)，保证了切片的连续性，一条带可长达60cm，连续标本片可达百余个(如触角横切)；③切片薄，采用该包埋技术，可切出1～12μ的连续切片，保证了对一些特殊构造(如各种感器)的连续切片观察；④溶点低，采用该剂渗透时，温度可在50℃～53℃一进行，避免了高温对生物组织的破坏作用；⑤取材方便，不用解剖，保证了器官的完整性及系统性，甚至可以进行整体切片(如小型个体)。该剂不但适于昆虫体壁的硬组织，也同时用于各种生物的软组织。
本发明采取优质松香，石蜡为原料，按比例松香∶石蜡＝1∶5～1∶0.15称重，入容器加热(120-150℃)，使混合剂溶解，再加入少量丙酮，叔丁醇，待溶液呈透明状，固化或入恒温箱备用。固化后呈黄褐色或棕褐色。如在室温为21～23℃条件下切片时，松香∶石蜡＝1∶1.5；24～26℃时，两者配比为1∶1。
Claims (3)
1、一种松香包埋剂，其特点是主要由优质松香、石蜡加入少量丙酮、叔丁醇混合而成，其中重量比松香∶石蜡＝1∶0.15-1∶5。
2、一种松香包埋剂的制作方法，其特征是主要由优质松香、石蜡加入少量丙酮、叔丁醇制作而成，其中重量比是松香∶石蜡＝1∶0.15-1∶5，混合入容器加热至120-150℃溶解，再加入少量丙酮、叔丁醇，使溶液呈透明状，固化后呈黄褐色或棕褐色，固化后放入恒温箱备用。
3、按权利要求2所述的松香包埋剂的制作方法，其特征是所说的松香、石蜡重量比在室温21-23℃条件下切片时为1∶1.5，在室温为24-26℃条件下切片时为1∶1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98125709A CN1102620C (zh) | 1998-12-09 | 1998-12-09 | 松香包埋剂及制作方法 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98125709A CN1102620C (zh) | 1998-12-09 | 1998-12-09 | 松香包埋剂及制作方法 |
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| Publication Number | Publication Date |
|---|---|
| CN1256291A CN1256291A (zh) | 2000-06-14 |
| CN1102620C true CN1102620C (zh) | 2003-03-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN98125709A Expired - Fee Related CN1102620C (zh) | 1998-12-09 | 1998-12-09 | 松香包埋剂及制作方法 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103759994B (zh) * | 2014-01-02 | 2015-12-09 | 河南科技大学 | 一种昆虫幼虫石蜡切片方法 |
| CN110204910A (zh) * | 2019-07-01 | 2019-09-06 | 北京服装学院 | 一种树脂复合材料及其制备方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069067A (zh) * | 1991-08-01 | 1993-02-17 | 于晓光 | 生物组织细胞包埋剂的生产工艺 |
| CN1072779A (zh) * | 1991-11-27 | 1993-06-02 | 武汉同济医科大学基础医学研究所 | 一种新的生物组织包埋剂和包埋方法 |
| CN1259532A (zh) * | 1998-10-30 | 2000-07-12 | 中国科学院新疆化学研究所 | 生物材料的电镜包埋剂 |
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1998
- 1998-12-09 CN CN98125709A patent/CN1102620C/zh not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069067A (zh) * | 1991-08-01 | 1993-02-17 | 于晓光 | 生物组织细胞包埋剂的生产工艺 |
| CN1072779A (zh) * | 1991-11-27 | 1993-06-02 | 武汉同济医科大学基础医学研究所 | 一种新的生物组织包埋剂和包埋方法 |
| CN1259532A (zh) * | 1998-10-30 | 2000-07-12 | 中国科学院新疆化学研究所 | 生物材料的电镜包埋剂 |
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| CN1256291A (zh) | 2000-06-14 |
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