CN110257245A - Nucleic acid detection reagent card - Google Patents
Nucleic acid detection reagent card Download PDFInfo
- Publication number
- CN110257245A CN110257245A CN201910647829.3A CN201910647829A CN110257245A CN 110257245 A CN110257245 A CN 110257245A CN 201910647829 A CN201910647829 A CN 201910647829A CN 110257245 A CN110257245 A CN 110257245A
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- chamber
- plunger shaft
- microchannel
- sap cavity
- sample
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- 238000001514 detection method Methods 0.000 title claims abstract description 118
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 82
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 71
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 71
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 71
- 239000007788 liquid Substances 0.000 claims abstract description 77
- 230000003321 amplification Effects 0.000 claims abstract description 45
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 45
- 238000000605 extraction Methods 0.000 claims abstract description 44
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- 239000012528 membrane Substances 0.000 claims description 37
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- 239000012807 PCR reagent Substances 0.000 claims description 29
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- 238000004140 cleaning Methods 0.000 claims description 12
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- 238000010586 diagram Methods 0.000 description 4
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- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
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- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- KSGDZBNTMXWYDK-UHFFFAOYSA-N 2-aminoethanol trihydrochloride Chemical compound Cl.Cl.Cl.NCCO KSGDZBNTMXWYDK-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- HMWVNKJRYWXJGS-UHFFFAOYSA-N C(C)(=O)OC=C.[C] Chemical compound C(C)(=O)OC=C.[C] HMWVNKJRYWXJGS-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000713869 Moloney murine leukemia virus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 description 1
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- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
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- 239000005030 aluminium foil Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
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- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The present invention provides a kind of nucleic acid detection reagent cards, it is related to detection of nucleic acids equipment technical field, the nucleic acid detection reagent card includes reagent card ontology, and sample area, extraction unit, amplification unit, detection unit, power unit and micro channel systems are provided on reagent card ontology；Power unit can select one with sample cell, extraction unit, amplification unit and the detection unit placed in sample area and be connected to；Power unit is for driving flowing of the liquid in micro channel systems.Three nucleic acid extraction of the present invention, amplification and detection steps can be completed in a reagent card, it is easy to operate, and it does not need multiple devices and carries out operation, the switch sample between multiple devices is not needed accordingly, detection time is not only shortened, and efficiently solves the problems, such as sample vulnerable to external contamination or sample environmental pollution.
Description
Technical field
The present invention relates to detection of nucleic acids equipment technical fields, more particularly, to a kind of nucleic acid detection reagent card.
Background technique
Detection of nucleic acids is very widely used in current clinical medicine domain, or even in forensic identification, ancestor genetic diseases
Goldstandard of the fields such as diagnosis, paternity test also as detection.
For the detection of nucleic acids of sample, it is generally divided into three nucleic acid extraction, nucleic acid amplification and detection of nucleic acids steps.Quotient at present
The nucleic acid detection reagent product of product, nucleic acid extraction, nucleic acid amplification and detection of nucleic acids are the mode independently carried out, preamble step mostly
Sample is moved in postorder equipment to the completion for carrying out subsequent step again, correspondingly, previous step cannot be effective after the completion of rapid
Ground is integrated with subsequent step and is carried out continuously.
Existing each step is independently performed nucleic acid detection reagent product, and each step requires independent equipment and completes, and one
Multiple devices are needed during secondary detection of nucleic acids, equipment occupation space is big；Further, it needs after the completion of previous step by sample
It is moved in postorder equipment, it is cumbersome, it takes a long time, it is higher to environment and personnel requirement；Meanwhile the reagent of non-integration produces
Product, when previous step is switched over to subsequent step, sample is in moving process also vulnerable to the pollution of external environment or dirt
Dye detection environment.
Summary of the invention
The purpose of the present invention is to provide a kind of nucleic acid detection reagent cards, to alleviate the detection of nucleic acids being commercialized at present examination
Agent product, nucleic acid extraction, amplification and detection need independent progress mostly, cannot effectively integrate previous step and subsequent step
It is integrated, detection of nucleic acids is caused to need multiple devices, cumbersome, the technical issues of taking a long time.
A kind of nucleic acid detection reagent card provided by the invention, including reagent card ontology are provided on the reagent card ontology
Sample area, extraction unit, amplification unit, detection unit, power unit and micro channel systems；
The sample area is provided with piercing mechanism, and the sample area is for placing sample cell, and the piercing mechanism is for piercing
Broken sample cell is to draw the sample in sample cell；
The power unit by the micro channel systems respectively with placed in the sample area sample cell, the extraction
Unit, amplification unit are connected to detection unit, and micro- between the sample cell placed in the power unit and the sample area
On channel, on the microchannel between the power unit and the extraction unit, the power unit and the amplification unit it
Between microchannel on and the microchannel between the power unit and the detection unit on valve is respectively set so that institute
Stating power unit can be with sample cell, the extraction unit, the amplification unit and the detection placed in the sample area
Unit selects a connection；
The power unit is for driving flowing of the liquid in the micro channel systems.
It further, further include washing accommodating chamber, the washing accommodating chamber is for placing cleaning solution；
The power unit is connected to by the micro channel systems with the washing accommodating chamber, and the washing accommodating chamber with
Valve is provided on microchannel between the power unit.
Further, liquid packet chamber is additionally provided on the reagent card ontology, the liquid packet chamber is placed with liquid packet, institute
It states and is loaded with redissolution liquid in liquid packet；
The power unit is connected to by the micro channel systems with the liquid packet.
Further, the power unit includes plunger shaft and piston, and the piston is arranged in the plunger shaft, described
Sample cell, the extraction unit, the amplification unit, the detection unit, liquid packet in plunger shaft and the sample area with
And the washing accommodating chamber is connected to by micro channel systems respectively, and between the sample cell in the plunger shaft and the sample area
Microchannel on, on the microchannel between the plunger shaft and the extraction unit, between the plunger shaft and amplification unit
It is micro- logical on microchannel on microchannel, between the plunger shaft and detection unit, between the plunger shaft and the liquid packet
On microchannel on road and between the plunger shaft and washing accommodating chamber, it is respectively arranged with valve.
Further, the extraction unit includes that cracking sap cavity and absorb-elute chamber, the cracking sap cavity are provided with sample
The reagent of cracking, the absorb-elute chamber are provided with trapping nucleic acids column；
The cracking sap cavity and the absorb-elute chamber are connected to the plunger shaft by the micro channel systems respectively, and
It is micro- on microchannel between the cracking sap cavity and the plunger shaft and between the absorb-elute chamber and the plunger shaft
Valve is respectively arranged on channel.
Further, the amplification unit includes PCR reagent chamber and PCR reaction chamber, and PCR reagent chamber and PCR reaction chamber divide
It is not connected to respectively by the micro channel systems with the plunger shaft, and micro- between the PCR reagent chamber and the plunger shaft
Valve is respectively arranged on microchannel on channel and between the PCR reaction chamber and the plunger shaft.
Further, the detection unit includes hybridization sap cavity, chip test chamber and magnetic particle chamber, the chip test chamber
Inside it is provided with detection chip；The hybridization sap cavity, the chip test chamber and the magnetic particle chamber respectively with the plunger shaft it
Between be connected to by the micro channel systems, and it is described hybridization sap cavity and the plunger shaft between microchannel on, the chip examine
It surveys on the microchannel between chamber and the plunger shaft and distinguishes on the microchannel between the magnetic particle chamber and the plunger shaft
It is provided with valve.
Further, it is described washing accommodating chamber quantity be three, respectively first washing sap cavity, second washing sap cavity and
Third washs sap cavity, and the first washing sap cavity, the second washing sap cavity and third washing sap cavity pass through with the plunger shaft respectively
Micro channel systems connection, and on the microchannel between the first washing sap cavity and the plunger shaft, second washing
On microchannel on microchannel between sap cavity and the plunger shaft and between third washing sap cavity and the plunger shaft
It is respectively arranged with valve.
Further, the sample area is sample cell room, and the piercing mechanism includes the first membrane puncture needl pipe and the second thorn film
The top of needle tubing, the first membrane puncture needl pipe and the second membrane puncture needl pipe is tip, and the tip height of the first membrane puncture needl pipe is big
In the tip height of the second membrane puncture needl pipe；
The lower end of the first membrane puncture needl pipe is communicated with the atmosphere, the lower end of the second membrane puncture needl pipe and the extraction unit
It is connected to by the micro channel systems.
Further, the reagent card ontology includes that pedestal, sealing counterdie, card chamber sealing teleblem and channel seal teleblem,
The sealing counterdie is elastic membrane；
The pedestal includes the bottom plate and functional block of integral structure, and the upper side of the bottom plate is divided into functional areas, PCR reaction
Area and chip detection zone, the functional block are arranged on the functional areas of the bottom plate, and piston groove is provided in the functional block, is split
Solve liquid bath, absorb-elute slot, PCR reagent slot, hybridization liquid bath, magnetic particle slot, sink and sample cell room；
Card chamber sealing teleblem is sealedly connected on the piston groove, the cracking liquid bath, the absorb-elute slot, described
The top of PCR reagent slot, the hybridization liquid bath, the magnetic particle slot and the sink, to form the plunger shaft, described
Sap cavity, the absorb-elute chamber, the PCR reagent chamber, the hybridization sap cavity, the magnetic particle chamber and the washing is cracked to hold
Receive chamber；
The channel sealing teleblem is sealedly connected on the PCR reaction zone of the bottom plate and the top of chip detection zone, described
Channel seals between teleblem and the bottom plate, and the PCR reaction is respectively formed on the PCR reaction zone and chip detection zone
Chamber and the chip test chamber；
The micro channel systems are arranged between sealing counterdie and the bottom surface of the bottom plate and channel sealing
Between teleblem and the bottom plate；
It is the piston groove, the cracking liquid bath, the attached elution slot, the PCR reagent slot, the hybridization liquid bath, described
First through hole is respectively arranged in the groove bottom of magnetic particle slot and the sink；The first through hole and the sealing counterdie
Microchannel between the bottom surface of the bottom plate is connected to；The PCR reaction zone and the chip detection zone are respectively arranged with up and down
Connection the second through-hole, second through-hole by it is described sealing counterdie and the bottom surface of the bottom plate between microchannel, Yi Jisuo
Channel sealing teleblem is stated to be connected to the microchannel between the bottom plate.
Further, it between the sealing counterdie and the first through hole, is supported by the sealing counterdie with first through hole
It connects or separates and form valve；
Between the sealing counterdie and second through-hole, by it is described sealing counterdie and second through-hole abutting or
Separation forms valve.
Nucleic acid detection reagent card provided by the invention includes reagent card ontology, and sample area is provided on reagent card ontology, is mentioned
Take unit, amplification unit, detection unit, power unit and micro channel systems.Wherein, power unit will by micro channel systems
Sample cell, extraction unit, the amplification unit placed in sample area are selected one with detection unit and are connected to, and corresponding power unit can be by sample
Product are transported to extraction unit from sample cell, and after extraction unit completes corresponding operating, power unit can be by sample from extraction unit
It is transported to amplification unit, after amplification unit completes corresponding operating, power unit again can convey sample from amplification unit sample
To detection unit, and final detection is completed in detection unit.The present invention by by sample area, extraction unit, amplification unit,
Detection unit is integrated on a reagent card ontology, and realizes the sample between each step by power unit and micro channel systems
Product conveying, three corresponding nucleic acid extraction, amplification and detection steps can be completed in a reagent card, easy to operate, and be not required to
It wants multiple devices to carry out operation, does not need the switch sample between multiple devices accordingly, not only shorten detection time, and
Efficiently avoid the problem of sample is vulnerable to external contamination or sample environmental pollution.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the whole perspective view of the explosion of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 2 is the schematic top plan view of the reagent card ontology of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 3 is the elevational schematic view of the reagent card ontology of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 4 is the sectional view of the sample area of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 5 is the schematic diagram of the liquid packet chamber of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 6 is the schematic illustration of the valve of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 7 is the schematic diagram of the absorb-elute chamber of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 8 is the structure top view of the PCR reaction chamber of nucleic acid detection reagent card provided in an embodiment of the present invention；
Fig. 9 is the cross-sectional view of Fig. 8；
Figure 10 is the schematic diagram of the plunger shaft of nucleic acid detection reagent card provided in an embodiment of the present invention；
Figure 11 is the schematic diagram of the chip test chamber of nucleic acid detection reagent card provided in an embodiment of the present invention.
Icon: 1- reagent card ontology；2- seals counterdie；The channel 3- seals teleblem；4- liquid packet；5- card chamber seals teleblem；
6- piston；7- detection chip；11- plunger shaft；12- cracks sap cavity；13- absorb-elute chamber；14- first washs sap cavity；15- second
Wash sap cavity；16- third washs sap cavity；17-PCR reagent chamber；18- hybridizes sap cavity；19- magnetic particle chamber；20- micro channel systems；
21- first through hole；The second through-hole of 22-；23- third through-hole；The sample area 100-；101- the first membrane puncture needl pipe；102- second pierces film
Needle tubing；131- trapping nucleic acids column；200- liquid packet chamber；201- puncture needle；The sample area 301- valve；302- cracks sap cavity valve；
303- absorb-elute chamber valve；304- first washs sap cavity valve；305- second washs sap cavity valve；306- third washs sap cavity
Valve；307-PCR reagent chamber valve；308- hybridizes sap cavity valve；309- magnetic particle chamber valve；310- liquid packet chamber valve；
The first PCR reaction zone valve of 311-；The 2nd PCR reaction zone valve of 312-；313- chip detection zone valve；330- compression bar；400-
PCR reaction chamber；500- chip test chamber.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The PCR being referred to herein refers to polymerase chain reaction.
With reference to shown in Fig. 1-Figure 11, the embodiment of the present invention provides a kind of nucleic acid detection reagent card, including reagent card ontology 1,
Sample area 100, extraction unit, amplification unit, detection unit, power unit and microchannel system are provided on reagent card ontology 1
System 20.
Wherein, sample area is provided with piercing mechanism, and sample area 100 is for placing sample cell, and piercing mechanism is for puncturing sample
Quality control is to draw the sample in sample cell.Power unit by micro channel systems 20 respectively with the sample placed in sample area 100
Quality control, extraction unit, amplification unit are connected to detection unit, and between the sample cell placed in power unit and sample area 100
Microchannel on, on the microchannel on the microchannel between power unit and extraction unit, between power unit and amplification unit,
And valve is respectively set on the microchannel between power unit and detection unit, so that power unit can be with sample area 100
Sample cell, extraction unit, the amplification unit of interior placement are selected one with detection unit and are connected to；Power unit is for driving liquid micro- logical
Flowing in road system 20.
In the present embodiment, power unit is single by the sample cell placed in sample area 100, extraction by micro channel systems 20
Member, amplification unit are selected one with detection unit and are connected to, and sample can be transported to extraction unit from sample cell by corresponding power unit,
After extraction unit completes corresponding operating, sample can be transported to amplification unit from extraction unit by power unit, and sample is single in amplification
After member completes corresponding operating, sample can be transported to detection unit from amplification unit again by power unit, and complete in detection unit
At final detection.The present invention is by being integrated in a reagent card for sample area 100, extraction unit, amplification unit, detection unit
On ontology 1, and realize by power unit and micro channel systems 20 connection of sample between each step, corresponding nucleic acid mentions
Taking, expand and detect three steps can complete in a reagent card, easy to operate, and does not need multiple devices and carry out operation,
The switch sample between multiple devices is not needed accordingly, not only shortens detection time, and it is easy to efficiently avoid sample
The problem of by external contamination or sample environmental pollution.
Specifically, extraction unit includes cracking sap cavity 12 and absorb-elute chamber 13, cracking sap cavity 12 is provided with sample and splits
The reagent of solution, absorb-elute chamber 13 are provided with trapping nucleic acids column 131.Cracking sap cavity 12 and absorb-elute chamber 13 divide with plunger shaft 11
Not Tong Guo micro channel systems 20 be connected to, and crack on the microchannel between sap cavity 12 and plunger shaft 11 and absorb-elute chamber 13
Valve is respectively arranged on microchannel between plunger shaft 11.Wherein it is preferred to which trapping nucleic acids column 131 uses silicon substrate plasma membrane
Material can capture nucleic acid molecules, when eluent rinses, discharge nucleic acid molecules.
Amplification unit includes PCR reagent chamber 17 and PCR reaction chamber 400, PCR reagent chamber 17 and PCR reaction chamber 400 and piston
Chamber 11 is connected to by micro channel systems 20 respectively, and on the microchannel between PCR reagent chamber 17 and plunger shaft 11 and PCR is anti-
It answers and is provided with valve on the microchannel between chamber 400 and plunger shaft 11.
Detection unit includes hybridization sap cavity 18, chip test chamber 500 and magnetic particle chamber 19, setting in chip test chamber 500
There is detection chip 7；Hybridization sap cavity 18, chip test chamber 500 and magnetic particle chamber 19 pass through microchannel between plunger shaft 11 respectively
System 20 is connected to, and is hybridized on the microchannel between sap cavity 18 and plunger shaft 11, between chip test chamber 500 and plunger shaft 11
Valve is respectively arranged on microchannel on microchannel and between magnetic particle chamber 19 and plunger shaft 11.
It as an alternative embodiment of the present invention, further include washing accommodating chamber on reagent card ontology 1, washing accommodating chamber is used
In placement cleaning solution；Power unit is connected to by micro- way system with washing accommodating chamber, and is washed between accommodating chamber and power unit
Microchannel on be provided with valve.
The quantity for washing accommodating chamber is three, and the respectively first washing sap cavity 14, second washs sap cavity 15 and third washing
Sap cavity 16, the first washing sap cavity 14, second washs sap cavity 15 and third washing sap cavity 16 passes through microchannel with plunger shaft 11 respectively
System 20 is connected to, and on the microchannel between the first washing sap cavity 14 and plunger shaft 11, the second washing sap cavity 15 and plunger shaft 11
Between microchannel on and third washing sap cavity 16 and plunger shaft 11 between microchannel on be respectively arranged with valve.
It should be noted that the quantity of washing accommodating chamber is not limited to provide in embodiment 3 forms, it can be as needed
Selection suitable quantity generally requires during current detection of nucleic acids and uses 3.
As another alternative embodiment of the invention, liquid packet chamber 200, liquid packet are additionally provided on reagent card ontology 1
Chamber 200 is placed with liquid packet 4, and redissolution liquid is loaded in liquid packet 4；Power unit passes through micro channel systems 20 and liquid packet 4
Connection.Redissolution liquid in general liquid packet is water, and liquid packet chamber 200 can choose whether to be arranged as needed, for example, first washes
Wash the reagent that sap cavity 14, second washs the functions chamber such as sap cavity 15 and third washing sap cavity 16, hybridization sap cavity 18, cracking sap cavity 12
Itself in fluid form in the presence of, liquid packet chamber 200 can be not provided with；15 and of sap cavity is washed in the first washing sap cavity 14, second
The reagent portions of functions chamber such as third washing sap cavity 16, hybridization sap cavity 18, cracking sap cavity 12 are all deposited with powdery form
When, need to be arranged liquid packet chamber 200 at this time.
As shown in figure 5, should also be provided with puncture needle 201 on liquid packet chamber 200, puncture needle is also the form of needle tubing,
Settable two, one of them is connect by micro channel systems 20 with plunger shaft 11 for puncturing liquid packet 4；Another with it is big
Gas connection, to maintain the air pressure in liquid packet 4.
A kind of nucleic acid detection reagent card of concrete form is provided below with reference to Fig. 1-Figure 11.
As shown in Figure 1, reagent card ontology 1 includes that pedestal, sealing counterdie 2, card chamber sealing teleblem 5 and channel seal teleblem 3,
And sealing counterdie 2 is elastic membrane.
Pedestal includes the bottom plate and functional block of integral structure, and the upper side on bottom plate is divided into functional areas, PCR reaction zone and core
Piece detection zone, functional block are arranged on the functional areas of bottom plate.Be provided in functional block piston groove, cracking liquid bath, absorb-elute slot,
PCR reagent slot, hybridization liquid bath, magnetic particle slot, the first sink, the second sink, third sink, liquid packet slot and sample cell
Room.Card chamber sealing teleblem is sealedly connected on piston groove, cracking liquid bath, absorb-elute slot, PCR reagent slot, hybridization liquid bath, magnetic particle
Slot, the first sink, the second sink, third sink, liquid packet slot top, with formed plunger shaft 11, cracking sap cavity 12,
Absorb-elute chamber 13, PCR reagent chamber 17, hybridization sap cavity 18, magnetic particle chamber 19, first wash sap cavity 14, second wash sap cavity 15,
Third washs sap cavity 16 and liquid packet chamber 200.
Card chamber seals teleblem 5 and uses double membrane structure, and upper layer film is aluminium foil, and lower membrane is semi-transparent membrane structure, breathes freely but not
Transflective liquid.Reagent card can tear upper layer film before, and lower membrane is bonded on reagent card ontology, prevent solution from overflowing, and generate
Pollution.
Channel sealing teleblem 3 is sealedly connected on the PCR reaction zone of bottom plate and the top of chip detection zone, specifically can be by viscous
The form of knot connects.Channel seals between teleblem 3 and bottom plate, and it is anti-on PCR reaction zone and chip detection zone to be respectively formed PCR
Answer chamber 400 and chip test chamber 500.
Sealing counterdie 2 is sealedly connected on the bottom surface of bottom plate, can specifically be connected by way of bonding, micro channel systems 20
It is arranged between sealing counterdie 2 and the bottom surface of bottom plate and channel seals between teleblem 3 and bottom plate；And piston groove, lysate
Slot, absorb-elute slot, PCR reagent slot, hybridization liquid bath, magnetic particle slot and sink groove bottom on be respectively arranged with first through hole
21；First through hole 21 with, sealing counterdie 2 be connected to the microchannel between bottom plate bottom surface；PCR reaction zone and chip detection zone difference
It is provided with the second through-hole 22 being connected to up and down, the second through-hole 22 will seal the microchannel between counterdie 2 and the bottom surface of bottom plate, and
Microchannel between channel sealing teleblem 3 and bottom plate is connected to.
Power unit includes plunger shaft 11 and piston 6, and piston 6 is arranged in plunger shaft 11, and 11 bottom of plunger shaft is provided with
12 third through-holes 23, plunger shaft 11 is connect by third through-hole 23 with micro channel systems 20, to realize plunger shaft 11 and sample
Sample cell, extraction unit, amplification unit, detection unit, liquid packet 4 in product area 100 and washing accommodating chamber are respectively communicated with.
Specifically, being provided with sample area valve 301, plunger shaft on microchannel between sample cell in plunger shaft 11 and sample area 100
Cracking sap cavity valve 302 is provided on microchannel between 11 and cracking sap cavity 12, between plunger shaft 11 and absorb-elute chamber 13
Microchannel on be provided with absorb-elute chamber valve 303, is arranged on the microchannel between plunger shaft 11 and the first washing sap cavity 14
There is the first washing sap cavity valve 304, is provided with the second cleaning solution on the microchannel between plunger shaft 11 and the second washing sap cavity 15
Chamber valve 305, plunger shaft 11 and third wash and are provided with third washing sap cavity valve 306 on the microchannel between sap cavity 16, living
Be provided with PCR reagent chamber valve 307 on microchannel between plug chamber 11 and PCR reagent chamber 17, plunger shaft 11 with hybridize sap cavity 18
Between microchannel on be provided with hybridization sap cavity valve 308, be provided on the microchannel between plunger shaft 11 and magnetic particle chamber 19
Magnetic particle chamber valve 309 is provided with liquid packet chamber valve 310, piston on the microchannel between plunger shaft 11 and liquid packet chamber 200
The first PCR reaction zone valve 311 and the 2nd PCR reaction zone valve are provided on microchannel between chamber 11 and PCR reaction chamber Room 400
Door 312 is provided with chip detection zone valve 313 on the microchannel between plunger shaft 11 and chip test chamber 500.
Specifically, sample area valve 301, cracking sap cavity valve 302, absorb-elute chamber valve 303, first wash sap cavity valve
Door 304, second washs sap cavity valve 305, third washing sap cavity valve 306, PCR reagent chamber valve 307, hybridization sap cavity valve
308, magnetic particle chamber valve 309 and liquid packet chamber valve 310 are arranged at the first through hole of sealing counterdie 2 and each cavity bottom
Between 21, and abut or separate to form valve with first through hole 21 by sealing counterdie 2.Specific principle is as shown in Figure 6.
First PCR reaction zone valve 311, the 2nd PCR reaction zone valve 312 and chip detection zone valve 313 are arranged at
It seals between counterdie 2 and the second through-hole 22, and by sealing counterdie 2 and the abutting of the second through-hole 22 or separates and to form valve.Tool
The principle of body can also refer to shown in Fig. 6.
Sample area valve 301 is used to control the injection of sample, cracking sap cavity valve 302, absorb-elute chamber valve 303, the
One washing sap cavity valve 304, second washs sap cavity valve 305, third washing sap cavity valve 306, PCR reagent chamber valve 307, miscellaneous
Sap cavity valve 308, magnetic particle chamber valve 309 and liquid packet chamber valve 310 is handed over to control its corresponding chamber and piston respectively
The connection of chamber 11.
Sample area 100 be sample cell room, piercing mechanism include the first membrane puncture needl pipe 101 and the second membrane puncture needl pipe 102, first
The top of membrane puncture needl pipe 101 and the second membrane puncture needl pipe 102 is tip, and the tip height of the first membrane puncture needl pipe 101 is greater than the
The tip height of two membrane puncture needl pipes 102；The lower end of first membrane puncture needl pipe 101 is communicated with the atmosphere, the lower end of the second membrane puncture needl pipe 102
It is connected to extraction unit by micro channel systems 20.
After sample cell is inserted into sample area 100, the first membrane puncture needl pipe 101 passes through micro channel systems 20 and through-hole and atmosphere
It communicates, the air pressure inside for balance sample pipe.Second membrane puncture needl pipe 102 passes through micro channel systems 20 and sample area valve 301
It is connected to plunger shaft 11, is used for pipette samples.The syringe needle section of first membrane puncture needl pipe 101 and the second membrane puncture needl pipe 102 is opposite, and
And the second membrane puncture needl pipe 102 is higher than by the first membrane puncture needl pipe 101, to prevent bubble to be inhaled into plunger shaft 11.Piston 6 is assembled in
In plunger shaft 11, can be moved up and down by external mechanical structure band piston 6 in plunger shaft 11, thus realize extract liquid and
Squeeze the effect of liquid.Also, by the collaborative work with each valve, the mobile liquid that can choose to each cavity and function
It can area.
PCR reaction chamber 400 is made of reagent card ontology 1 and channel sealing teleblem 3, and there is the first PCR reaction zone valve in front and back
311 and the 2nd PCR reaction zone valve, in PCR reaction process, the first PCR reaction zone valve 311 and the 2nd PCR reaction zone valve
Door 312 is closed, and seal cavity is formed；Efficiently avoid the pollution of aerosol.
The present embodiment is by the extruding of valve and loosens and controls the mutual of plunger shaft 11 and reagent chamber, each chamber in functional areas
Then connection provides liquid using the up and down motion of piston 6 and shifts required power between each chamber and functional areas, thus
Complete the operation such as cracking, washing, absorption, elution, PCR amplification, nucleic acid hybridization check.Specifically, when using 330 squeezing valve of compression bar
When the channel in door region seals counterdie 2, sealing counterdie 2 is abutted with the through-hole of corresponding cavity bottom, so that cavity bottom is logical
The bottom in hole blocks namely valve is closed, and plunger shaft 11 and reagent chamber and functional areas disconnect.When the channel for loosening pressure valve region
It when sealing counterdie 2, seals counterdie 2 and the through-hole of corresponding cavity bottom is separate, valve is opened, plunger shaft 11 and reagent chamber and function
It can Qu Liantong.It should be noted that during once-through operation, plunger shaft 11 only can with one of chamber, for example,
In sample introduction product, other than sample area valve 301 is opened, other valves are in closed state, at this time pull piston 6, can
Sample is extracted out of sample cell to plunger shaft 11；Then, sample area valve 301 is closed, it is corresponding to open cracking sap cavity
Sample in plunger shaft 11 is injected into cracking sap cavity 12 by pushing piston 6 by valve 302.The samples of other processes shift or
Liquid transfer, similar when to sample introduction product, details are not described herein.
In the present embodiment, the reagent cracked in sap cavity 12 can be guanidinium isothiocyanate, three (methylol) aminomethanes
(Tris-HCl), ethylenediamine tetra-acetic acid (EDTA), Triton X-100 (Trition-X100), lauryl sodium sulfate
(SDS) aqueous solution, the first cleaning solution can be guanidinium isothiocyanate, three (methylol) aminomethane (Tris) aqueous solutions, second
Cleaning solution can be two kinds, and one is the formulas without freeze-drying: ethyl alcohol, sodium chloride, three (methylol) aminomethane (Tris-
HCl solution), another kind are the formulas that can be lyophilized: sodium chloride, polyethylene glycol (PEG) 8000, three (methylol) aminomethane
(Tris-HCl) solution；Third washs the buffer that the liquid in sap cavity 16 is sodium bicarbonate, sodium carbonate, Tween-20, PCR
The reagent of reagent chamber 17: (Taq enzyme is from thermus aquaticus Thermus for amplimer, MMLV reverse transcriptase, Taq enzyme
The archaeal dna polymerase with thermal stability isolated in Aquaticus), dNTPs (deoxyribonucleotide), ammonium sulfate, chlorination
The Tris such as magnesium (the Tris Chinese name of an article is trishydroxymethylaminomethane) buffer.The reagent for hybridizing sap cavity 18 can be two kinds, a kind of
Formula without freeze-drying: formamide, lauryl sodium sulfate (SDS), sodium citrate buffer solution, the formula that can be lyophilized: carbon
Vinyl acetate, lauryl sodium sulfate (SDS), sodium citrate buffer solution.The solution of magnetic particle chamber 19 is magnetic bead, bovine serum albumin
White (BSA), carbonate buffer solution.
Detection chip 7 can detect nucleic acid by detecting magnetosensitive Crossing system, but be not limited to magnetosensitive Crossing system
Nucleic acid is detected.Preferably, detection chip 7 uses giant magnetoresistance chip, and the fixed hybridization probe in surface can be with band after amplification
There is the target nucleic acid of biotin group to be hybridized, is allowed to be incorporated in chip surface, the magnetism on Streptavidin surface is received later
For rice grain by biotin-avidin reaction bonded in chip surface, giant magnetoresistance chip is fixed on chip surface by detection
The magnetic signal that magnetic nanoparticle generates detects nucleic acid.
Preferred in the present embodiment, nucleic acid extraction, amplification and detection reagent are encapsulated in reagent card using freeze-dried powder form.
By the extruding of valve and loosen and control being interconnected for plunger shaft 11 and reagent chamber and functional areas, then utilizes
The up and down motion transfer liquid of piston 6 is to each chamber and functional areas.It is given below the one of the present embodiment nucleic acid detection reagent card
A specific application process: whole use process:
Step 1: redissolving reagent process
Solution 50ul, 20ul, 300ul, 300ul, 300ul in liquid packet 4 are drawn using piston 6 respectively, are injected separately into
Hybridize sap cavity 18, magnetic particle chamber 19, the first washing sap cavity, the second washing sap cavity, third washing sap cavity；Piston carries out once every time
The absorption of liquid carries out the injection of a liquid every time, and during piston executes liquid injection, can move up and down piston
6 pairs of reagents therein are quickly redissolved.
Step 2: nucleic acid extraction process
Sample tube is inserted into sample tube bonding pad, draws 200ul sample using piston 6 and is injected into cracking sap cavity, moves up and down
6 mode of piston redissolves lysate, which cracks sample.Ultrasound can also be added in lysate bottom of chamber portion in cracking process
Processing improves lysis efficiency.
After cracking, cracking mixed liquor is injected into using piston 6 by absorb-elute chamber, is made in nucleic acid absorption to adsorption column, given up
Liquid is injected back into cracking sap cavity.Then the cleaning of the first cleaning solution and the second cleaning solution is drawn respectively by piston to carry out repeatedly adsorption column
Cleaning, waste liquid are injected back into cracking sap cavity.Finally draw solution 50ul is injected into absorb-elute chamber from liquid packet 4, carries out to nucleic acid
Elution.
Step 3: PCR amplification process:
Eluent is drawn using piston 6 and is injected into the redissolution PCR reagent of PCR reagent chamber 17, piston 6 is moved up and down, is allowed to multiple
It is molten uniform.Then dissolved PCR reagent mixed liquor is injected into PCR reaction zone.By 30 heating down cycles, core is completed
The amplification procedure of acid.
Step 4: hybridization detection process
Using piston 6 the PCR solution sucker chamber 11 after amplification, hybridization solution 100ul is then drawn, is mixed with
Chip detection zone is injected into after even.Chip surface fix hybridization probe, can with after amplification have biotin group target core
Acid is hybridized, and is allowed to be incorporated in chip surface.Later, piston 6 draws third cleaning solution and cleans chip surface, is then injected into chain
The magnetic nanoparticle on mould Avidin surface is huge by biotin-avidin reaction bonded in chip surface to chip detection zone
Magnetoresistive chip detects nucleic acid by the magnetic signal that the magnetic nanoparticle that detection is fixed on chip surface generates.
Detection chip of the embodiment of the present invention is encapsulated in micro channel systems, can by magnetosensitive Crossing system to nucleic acid into
Row detection.The present invention realizes that nucleic acid extraction, amplification and detection are integrated in a reagent card, not only simplifies operating process, contracts
The short time reduces reaction volume, realizes automatic detection purpose, and efficiently avoid external contamination and aerosol
Pollution.Meanwhile sample dosage is reduced using microflow control technique and magnetosensitive hybridization technique, improve nucleic acid extraction and amplification
Efficiency improves detection sensitivity and specificity.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (11)
1. a kind of nucleic acid detection reagent card, which is characterized in that including reagent card ontology, be provided with sample on the reagent card ontology
Area, extraction unit, amplification unit, detection unit, power unit and micro channel systems；
The sample area is provided with piercing mechanism, and the sample area is for placing sample cell, and the piercing mechanism is for puncturing sample
Quality control is to draw the sample in sample cell；
The power unit is single with the sample cell, the extraction placed in the sample area respectively by the micro channel systems
Member, amplification unit are connected to detection unit, and micro- logical between the sample cell placed in the power unit and the sample area
On road, on the microchannel between the power unit and the extraction unit, between the power unit and the amplification unit
Microchannel on and the microchannel between the power unit and the detection unit on valve is respectively set so that described
Power unit can be single with the sample cell, the extraction unit, the amplification unit and the detection placed in the sample area
Member selects a connection；
The power unit is for driving flowing of the liquid in the micro channel systems.
2. nucleic acid detection reagent card according to claim 1, which is characterized in that further include washing accommodating chamber, the washing
Accommodating chamber is for placing cleaning solution；
The power unit is connected to by the micro channel systems with the washing accommodating chamber, and the washing accommodating chamber with it is described
Valve is provided on microchannel between power unit.
3. nucleic acid detection reagent card according to claim 2, which is characterized in that be additionally provided with liquid on the reagent card ontology
Body packet chamber, the liquid packet chamber are placed with liquid packet, are loaded with redissolution liquid in the liquid packet；
The power unit is connected to by the micro channel systems with the liquid packet.
4. nucleic acid detection reagent card according to claim 2 or 3, which is characterized in that the power unit includes plunger shaft
And piston, the piston are arranged in the plunger shaft, the sample cell, the extraction in the plunger shaft and the sample area are single
First, the described amplification unit, the detection unit, liquid packet and the washing accommodating chamber are connected to by micro channel systems respectively,
And on the microchannel between the sample cell in the plunger shaft and the sample area, between the plunger shaft and the extraction unit
Microchannel on, it is micro- logical on the microchannel between the plunger shaft and amplification unit, between the plunger shaft and detection unit
It is micro- on microchannel on road, between the plunger shaft and the liquid packet and between the plunger shaft and washing accommodating chamber
On channel, it is respectively arranged with valve.
5. nucleic acid detection reagent card according to claim 4, which is characterized in that the extraction unit include cracking sap cavity and
Absorb-elute chamber, the cracking sap cavity are provided with the reagent of sample dissociation, and the absorb-elute chamber is provided with trapping nucleic acids column；
The cracking sap cavity and the absorb-elute chamber are connected to the plunger shaft by the micro channel systems respectively, and described
On microchannel between the cracking sap cavity and the plunger shaft and microchannel between the absorb-elute chamber and the plunger shaft
On be respectively arranged with valve.
6. nucleic acid detection reagent card according to claim 5, which is characterized in that the amplification unit includes PCR reagent chamber
With PCR reaction chamber, PCR reagent chamber and PCR reaction chamber are connected to the plunger shaft by the micro channel systems respectively, and described
Microchannel on microchannel between PCR reagent chamber and the plunger shaft and between the PCR reaction chamber and the plunger shaft
On be respectively arranged with valve.
7. nucleic acid detection reagent card according to claim 6, which is characterized in that the detection unit include hybridization sap cavity,
Chip test chamber and magnetic particle chamber are provided with detection chip in the chip test chamber；The hybridization sap cavity, chip detection
Chamber and the magnetic particle chamber are connected between the plunger shaft by the micro channel systems respectively, and the hybridization sap cavity and institute
It states on the microchannel on the microchannel between plunger shaft, between the chip test chamber and the plunger shaft and the magnetic
Valve is respectively arranged on microchannel between grain chamber and the plunger shaft.
8. nucleic acid detection reagent card according to claim 7, which is characterized in that the quantity of the washing accommodating chamber is three
A, the respectively first washing sap cavity, the second washing sap cavity and third wash sap cavity, the first washing sap cavity, the second cleaning solution
Chamber and third washing sap cavity are connected to the plunger shaft by the micro channel systems respectively, and the first washing sap cavity and institute
It states on the microchannel between plunger shaft, on the microchannel between the second washing sap cavity and the plunger shaft and described the
Valve is respectively arranged on microchannel between three washing sap cavities and the plunger shaft.
9. nucleic acid detection reagent card according to claim 1, which is characterized in that the sample area is sample cell room, described
Piercing mechanism includes the first membrane puncture needl pipe and the second membrane puncture needl pipe, and the top of the first membrane puncture needl pipe and the second membrane puncture needl pipe is equal
For tip, and the tip height of the first membrane puncture needl pipe is greater than the tip height of the second membrane puncture needl pipe；
The lower end of the first membrane puncture needl pipe is communicated with the atmosphere, and lower end and the extraction unit of the second membrane puncture needl pipe pass through
The micro channel systems connection.
10. nucleic acid detection reagent card according to claim 7, which is characterized in that the reagent card ontology includes pedestal, close
Back cover film, card chamber sealing teleblem and channel seal teleblem, and the sealing counterdie is elastic membrane；
The pedestal includes the bottom plate and functional block of integral structure, the upper side of the bottom plate be divided into functional areas, PCR reaction zone and
Chip detection zone, the functional block are arranged on the functional areas of the bottom plate, and piston groove, lysate are provided in the functional block
Slot, absorb-elute slot, PCR reagent slot, hybridization liquid bath, magnetic particle slot, sink and sample cell room；
The card chamber sealing teleblem is sealedly connected on the piston groove, the cracking liquid bath, the absorb-elute slot, the PCR
The top of reagent trough, the hybridization liquid bath, the magnetic particle slot and the sink, to form the plunger shaft, described split
Sap cavity, the absorb-elute chamber, the PCR reagent chamber, the hybridization sap cavity, the magnetic particle chamber and the washing is solved to accommodate
Chamber；
The channel sealing teleblem is sealedly connected on the PCR reaction zone of the bottom plate and the top of chip detection zone, the channel
Seal between teleblem and the bottom plate, and be respectively formed on the PCR reaction zone and chip detection zone the PCR reaction chamber and
The chip test chamber；
The micro channel systems are arranged between sealing counterdie and the bottom surface of the bottom plate and channel sealing teleblem
Between the bottom plate；
The piston groove, the cracking liquid bath, the attached elution slot, the PCR reagent slot, the hybridization liquid bath, the magnetic
First through hole is respectively arranged in the groove bottom of grain slot and the sink；The first through hole and the sealing counterdie and institute
State the microchannel connection between the bottom surface of bottom plate；The PCR reaction zone is respectively arranged with the chip detection zone and is connected to up and down
The second through-hole, second through-hole is by microchannel between the sealing counterdie and the bottom surface of the bottom plate and described logical
Road sealing teleblem is connected to the microchannel between the bottom plate.
11. nucleic acid detection reagent card according to claim 10, which is characterized in that the sealing counterdie is logical with described first
Between hole, abut or separate to form valve with first through hole by the sealing counterdie；
Between the sealing counterdie and second through-hole, by the sealing counterdie and the abutting of second through-hole or separate
Form valve.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910647829.3A CN110257245A (en) | 2019-07-16 | 2019-07-16 | Nucleic acid detection reagent card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910647829.3A CN110257245A (en) | 2019-07-16 | 2019-07-16 | Nucleic acid detection reagent card |
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| CN110964715A (en) * | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
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