CN110250001A - Wild paper mulberry resource tissue cultures quickly expand a kind of numerous method - Google Patents
Wild paper mulberry resource tissue cultures quickly expand a kind of numerous method Download PDFInfo
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- CN110250001A CN110250001A CN201910607407.3A CN201910607407A CN110250001A CN 110250001 A CN110250001 A CN 110250001A CN 201910607407 A CN201910607407 A CN 201910607407A CN 110250001 A CN110250001 A CN 110250001A
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- paper mulberry
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Abstract
Denomination of invention: " wild paper mulberry resource tissue cultures quickly expand a kind of numerous method ".Technical field: field of plant tissue culture technique.Main technical problems to be solved: wild resource " paper mulberry " method for tissue culture has not been reported at present, belongs to technological gap item.Inventor is resolved the Problem Technical Solution main points by experimental verification: the explant materials time appropriate there is provided the wild resource, being conducive to ensure the resource clump bud differentiation capability and healthy growing way；Two there is provided the sterilization method of the resource explant, is conducive to removal virus or bacterium infection hinders culture process；Three there is provided optimal medium formula (containing " the MS culture medium of 1.0mg/L 6-BA and 0.1mg/L IBA "), is conducive to the robust growth for inducing more clump buds generations and plant；Four, there is provided the resource aseptic seedling tissue cultures optimum growh environmental index data, are conducive to the fast-propagation culture of the resource.
Description
Technical field
The invention belongs to technical field of tissue culture, and in particular to paper mulberry, small paper mulberry (Broussonetia
KazinokiSieb. et Zucc.) (also known as: paper mulberry skin, leatheroid, spire structure skin bavin, Pi Teng, paddy skin rattan, Portugal coil, female paddy, wild structure
Tree, wild structure peach, kazinoki papermulberry branchlet and leaf) and the induction of this species chimera (aneuploid, polyploid) the quick expanding propagation method of tissue cultures.
Background technique
Paper mulberry, small paper mulberry (Broussonetia kazinokiSieb. et Zucc.), is under the jurisdiction of Moraceae Moraceae Sang Ya
Moroideae paper mulberry race, section Broussonetieae, collective fruits is spherical, diameter 5~6(~10) mm, meat is red, containing there are many rouge
41 kinds of ingredients of fat acids and lipid, can be used as tonic, have consumer edema, strengthen the bone, improving eyesight, stomach invigorating, beauty and other effects.In recent years it grinds
Study carefully and show that it has improvement result for memory, can be used for treating senile dementia.Paper mulberry bark is flexible, and fiber is long, and pulling force is strong, there is silk
Light, color pure white can make advanced blended raw material, also can singly spin, can synthetic cotton processed, paraffin paper, can also brown paper processed and other advanced
Paper, straw sandals, rope etc., root has antipyretic and antidote functions, can control traumatic injury.Paper mulberry timber yellow-white, it is light and soft, case can be made
Plate and for charcoal firewood.Bark toughness is strong, pulling force is big, can synthetic cotton processed, make blended and single spin fiber, cotton paper, a kind of white tissue paper and north processed
Square rice paper is used;Also it can be used for fiber crops, for rope making rope, gunnysack.The male inflorescence of paper mulberry has larger containing more protein, amino acid
Nutritive value, noodles served with soy sauce, sesame butter, etc. can steamed wheaten foods, leaf is also capable of using as feed.Paper mulberry also has an extensive medical usage, leaf smash juice clothes can control nosebleed epistaxis,
Dysentery is controlled, leaf soaking object, which can be used for crops, to be prevented eliminating aphis;The colloid of leaf is erasable to control tinea sore.Milk in leaf and bark can be controlled
Snake, worm, bee, scorpion, dog bite.The paper mulberry pyrrolizidine alkaloid A~Q and paper mulberry alkali W, X that are rich in paper mulberry (small paper mulberry), M1, U1,
The discovery of J1, J2 and structure peaceful alkali A, B, function effect are just gradually taken seriously and develop, but limited wild resource is difficult
To meet the needs of mass production, the census operations that inventor has participated in fourth national chinese medicine resource investigation Xuanwei team obtains this
After species wild resource, the tissue culture quick breeding system and the exploratory development of multiploid induction of this species are unfolded at once, by more
It takes turns experimental exploration and analyzes the verifying again of result, obtain wild paper mulberry resource and this species chimera (aneuploid, polyploid)
Tissue cultures quickly expand numerous method.
Summary of the invention
Wild resource " paper mulberry " tissue cultures provided by the invention quickly expand a kind of numerous method, and steps are as follows:
(1) it draws materials: selecting healthy and strong plant (no disease and pests harm), Yu Qingtian 9:00~16:00 period wins young tender stem tip.
(2) sterilize: after flowing water rinses 30 points of kinds, 75% alcohol disinfecting 30 seconds, 0.1% mercuric chloride sterilizes 8~15 points of kinds, and use is sterile
Water flushing 3~5 times spare.
(3) it cultivates: the MS that the explant disinfected is inoculated in 6-BA containing 1.0mg/L and 0.1mg/L IBA is cultivated
It is 25 ± 2 DEG C in temperature, illumination (1000~2000Lx) is cultivated in base.
Preferably, materials should be preferable in spring in step (1).
Preferably, flowing water, which rinses, is added appropriate dish washing liquid, and disinfection treatment should be complete on superclean bench in step (2)
At, and suitably give ultraviolet light irradiation.
Preferably, in step (3), inoculation material 1~2 blade of band, advantageous plant strain growth when cutting.In addition, training
Feeding base also contains 30g/L sucrose and 6.5g agar, and PH is adjusted to 5.8, avoids direct sunlight.
Claims (2)
1. present invention culture species are entitled " paper mulberry ", " small paper mulberry (Broussonetia kazinokiSieb. et Zucc.) "
(also known as: " paper mulberry skin, leatheroid, spire structure skin bavin, Pi Teng, paddy skin rattan, Portugal coil, female paddy, wild paper mulberry, wild structure peach, kazinoki papermulberry branchlet and leaf "), it adapts to
Range includes wild resource, cultivation resource and the aneuploid and polyploid resource induced on this basis of this species.
2. the invention belongs to technical field of tissue culture, before fast-propagation by material source environment (such as: height above sea level, weather, soil
The factors such as earth or callus differentiation microenvironment) influence, when causing the expression of resource inhereditary feature not consistent, so to culture
Base MS interior element, sugar, 6-BA, IBA plus-minus and addition (elements such as NAA, VB, VC, GA) belong to interest field of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910607407.3A CN110250001A (en) | 2019-07-08 | 2019-07-08 | Wild paper mulberry resource tissue cultures quickly expand a kind of numerous method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910607407.3A CN110250001A (en) | 2019-07-08 | 2019-07-08 | Wild paper mulberry resource tissue cultures quickly expand a kind of numerous method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110250001A true CN110250001A (en) | 2019-09-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910607407.3A Pending CN110250001A (en) | 2019-07-08 | 2019-07-08 | Wild paper mulberry resource tissue cultures quickly expand a kind of numerous method |
Country Status (1)
| Country | Link |
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| CN (1) | CN110250001A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633738A (en) * | 2018-05-15 | 2018-10-12 | 贵州务川科华生物科技有限公司 | A method of producing paper mulberry tissue-cultured seedling |
| CN108834904A (en) * | 2018-08-21 | 2018-11-20 | 云南兴构农业产业发展有限公司 | The culture medium and breeding method of paper mulberry are cultivated for root tissue |
| CN109220800A (en) * | 2018-10-18 | 2019-01-18 | 楚雄师范学院 | A method of effectively improving hybridization paper mulberry tissue-cultured seedling rooting rate |
-
2019
- 2019-07-08 CN CN201910607407.3A patent/CN110250001A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633738A (en) * | 2018-05-15 | 2018-10-12 | 贵州务川科华生物科技有限公司 | A method of producing paper mulberry tissue-cultured seedling |
| CN108834904A (en) * | 2018-08-21 | 2018-11-20 | 云南兴构农业产业发展有限公司 | The culture medium and breeding method of paper mulberry are cultivated for root tissue |
| CN109220800A (en) * | 2018-10-18 | 2019-01-18 | 楚雄师范学院 | A method of effectively improving hybridization paper mulberry tissue-cultured seedling rooting rate |
Non-Patent Citations (2)
| Title |
|---|
| SIMING CUI等: "An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants", 《OPEN LIFE SCIENCES》 * |
| 宋丽红等: "光叶楮的组织培养和快速繁殖", 《植物生理学通讯》 * |
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Application publication date: 20190920 |
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