CN110237115A - The preparation of Fructus Trichosanthis extract and its application in development anti esophageal cancer drug - Google Patents
The preparation of Fructus Trichosanthis extract and its application in development anti esophageal cancer drug Download PDFInfo
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- CN110237115A CN110237115A CN201910695574.8A CN201910695574A CN110237115A CN 110237115 A CN110237115 A CN 110237115A CN 201910695574 A CN201910695574 A CN 201910695574A CN 110237115 A CN110237115 A CN 110237115A
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- fructus trichosanthis
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- trichosanthis extract
- esophageal cancer
- ethyl acetate
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- 239000000284 extract Substances 0.000 title claims abstract description 33
- 208000000461 Esophageal Neoplasms Diseases 0.000 title claims abstract description 17
- 206010030155 Oesophageal carcinoma Diseases 0.000 title claims abstract description 17
- 201000004101 esophageal cancer Diseases 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000003560 cancer drug Substances 0.000 title claims abstract description 10
- 238000011161 development Methods 0.000 title description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 53
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 235000019441 ethanol Nutrition 0.000 claims abstract description 21
- 235000008326 Trichosanthes anguina Nutrition 0.000 claims abstract description 12
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- 238000001035 drying Methods 0.000 claims abstract description 9
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- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000003810 ethyl acetate extraction Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 4
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- 239000000243 solution Substances 0.000 description 28
- 238000006243 chemical reaction Methods 0.000 description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 12
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- -1 flavone compound Chemical class 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 241001062009 Indigofera Species 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
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- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
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- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
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- PNEFMUGLHZEHKK-UHFFFAOYSA-N Cl.NO.CCO Chemical compound Cl.NO.CCO PNEFMUGLHZEHKK-UHFFFAOYSA-N 0.000 description 1
- PQMOXTJVIYEOQL-UHFFFAOYSA-N Cumarin Natural products CC(C)=CCC1=C(O)C(C(=O)C(C)CC)=C(O)C2=C1OC(=O)C=C2CCC PQMOXTJVIYEOQL-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- FSOGIJPGPZWNGO-UHFFFAOYSA-N Meomammein Natural products CCC(C)C(=O)C1=C(O)C(CC=C(C)C)=C(O)C2=C1OC(=O)C=C2CCC FSOGIJPGPZWNGO-UHFFFAOYSA-N 0.000 description 1
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- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
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- 239000003146 anticoagulant agent Substances 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
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- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- 230000003637 steroidlike Effects 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
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- 239000012085 test solution Substances 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
- A61K36/428—Trichosanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation of Fructus Trichosanthis extract and its developing the application in anti esophageal cancer drug, preparation method includes the following steps: S1, impregnates 75% ethyl alcohol of Snakegourd Fruit 24 hours, Snakegourd Fruit is to take the beginning of September i.e. by mature Snakegourd Fruit, slice, drying, small fire is fried to golden yellow, and spare obtain is crushed；S2, it is filtered immediately after being heated to reflux 2 hours, in triplicate, merges liquid phase；S3, liquid phase obtained by step S2 is concentrated into no ethyl alcohol, ethyl acetate extraction then plus in equal volume；Ethyl acetate phase is stayed after the completion of S4, extraction, recycling ethyl acetate is evaporated to obtain Fructus Trichosanthis extract.The Fructus Trichosanthis extract being prepared using the preparation method can be applied in anti esophageal cancer drug.
Description
Technical field
The present invention relates to technical field of Chinese medicine, and in particular to a kind of preparation of Fructus Trichosanthis extract and its is developing anti-oesophagus
Application in cancer drug.
Background technique
Tumour is one of great killer of human health, is a kind of common disease and frequently-occurring disease for seriously threatening human health,
Its disease incidence is in rising trend.Currently, Chinese medicine, compared with chemicals, chemicals are significant in efficacy, but erious adverse reaction, easily produce
Raw drug resistance.Adverse reaction of tcm is small, is mitigating patient clinical symptom, is improving patient's immunity and life quality, mitigation chemotherapy
Adverse reaction and pre- preventing tumor etc. show apparent advantage, have played important function.It finds and digs from Chinese herbal medicine
Efficient, low toxicity, wide spectrum, cheap anticancer drug are dug, there is good prospect, is the direction that vast work of Chinese medicine person makes great efforts.
Summary of the invention
In view of the deficiencies of the prior art, the present invention is intended to provide the preparation of a kind of Fructus Trichosanthis extract and its in Anti-esophageal cancer drug
Application in object.
To achieve the goals above, the present invention adopts the following technical scheme:
The present invention is intended to provide a kind of Fructus Trichosanthis extract is developing the application in anti esophageal cancer drug.
Further, the preparation method of the Fructus Trichosanthis extract includes the following steps:
S1,75% ethyl alcohol of Snakegourd Fruit is impregnated 24 hours；
S2, it is filtered immediately after being heated to reflux 2 hours, in triplicate, merges liquid phase；
S3, liquid phase obtained by step S2 is concentrated into no ethyl alcohol, ethyl acetate extraction then plus in equal volume；
Ethyl acetate phase is stayed after the completion of S4, extraction, recycling ethyl acetate is evaporated to obtain Fructus Trichosanthis extract.
Further, the preparation process of the Snakegourd Fruit in step S1 are as follows: take the beginning of September by mature Snakegourd Fruit, to be sliced, dry
Dry, small fire is fried to golden yellow, is then crushed spare.
The beneficial effects of the present invention are:
The present invention provides a kind of preparation method of Fructus Trichosanthis extract, and is experimentally confirmed, and Fructus Trichosanthis extract is in anti-food
Remarkable effect can be played in pipe cancer, can be used for preparing anti esophageal cancer drug.
Detailed description of the invention
Fig. 1 is various concentration extract in the present embodiment 3 to the influence schematic diagram of esophageal cancer cell EC9706 cell proliferation；
Fig. 2 is various concentration extract in the present embodiment 3 to the influence schematic diagram of esophageal cancer cell TE1 cell proliferation；
Fig. 3 is various concentration extract in the present embodiment 3 to the influence schematic diagram of esophageal cancer cell EC109 cell proliferation.
Specific embodiment
Below with reference to attached drawing, the invention will be further described, it should be noted that the present embodiment is with this technology side
Premised on case, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to this reality
Apply example.
Embodiment 1
The present embodiment provides a kind of Fructus Trichosanthis extracts to develop the application in anti esophageal cancer drug.
The preparation method of the Fructus Trichosanthis extract, includes the following steps:
S1,75% ethyl alcohol of Snakegourd Fruit is impregnated 24 hours；
S2, it is filtered immediately after being heated to reflux 2 hours, in triplicate, merges liquid phase；
S3, liquid phase obtained by step S2 is concentrated into no ethyl alcohol, ethyl acetate extraction then plus in equal volume；
Ethyl acetate phase is stayed after the completion of S4, extraction, recycling ethyl acetate is evaporated to obtain Fructus Trichosanthis extract.
Further, the preparation process of the Snakegourd Fruit in step S1 are as follows: take the beginning of September by mature Snakegourd Fruit, to be sliced, drying,
Small fire is fried to golden yellow, is then crushed spare.
Embodiment 2
The present embodiment is intended to verify the effective component of Fructus Trichosanthis extract prepared by embodiment 1 by experiment.
2.1, sample solution is prepared
2.1.1, aqueous solution: weighing 1 gained Fructus Trichosanthis extract of embodiment, set in stuffed conical flask, adds water, sonic oscillation point
Clock makes sufficiently to dissolve, and filtering collects filtrate, obtains sample aqueous solution.
2.1.2, ethanol solution: weighing 1 gained Fructus Trichosanthis extract of embodiment, set in stuffed conical flask, adds ethyl alcohol, ultrasound vibration
It swings minute, filters, collect filtrate, obtain sample ethanol solution.
2.1.3, chloroformic solution:: 1 gained Fructus Trichosanthis extract of embodiment is weighed, is set in stuffed conical flask, chlorination is imitative, ultrasound
Vibration, filtering collect filtrate, obtain sample chloroformic solution.
2.2, verification test
2.2.1 flavone compound
2.2.1.1, one magnesium powder of hydrochloric acid is tested: sample ethanol solution is taken, after adding a small amount of magnesium powder, concentrated hydrochloric acid one is added dropwise and drips, it is molten
Liquid color reddens, positive reaction.
2.2.1.2, one zinc powder of hydrochloric acid is tested: being taken sample ethanol solution, after adding a small amount of zinc powder, concentrated hydrochloric acid one is added dropwise and drips, puts
It postpones solution colour to redden, positive reaction.
2.2.1.3, alchlor fluorescent test: taking sample ethanol solution appropriate, and point is on filter paper, after drying, spray with
Alchlor ethanol solution is set and is observed under ultraviolet lamp, and yellow fluorescence, positive reaction is presented in sample point.
(alchlor is produced in matching for alchlor ethanol solution, and ethyl alcohol is added to dissolve to obtain the final product).
2.2.1.4, concentrated ammonia liquor fluorescent test: taking sample ethanol solution appropriate, and point after drying, sets ammonia steaming on filter paper
A few minutes are smoked in gas, sets and observes under ultraviolet lamp, and yellow fluorescence, positive reaction is presented in sample point.
2.2.2, cumarin, lactone compound
2.2.2.1, Open-closed-loop reacts: taking ethanol solution, adding sodium hydroxide solution one drips, and solution is clarification, then plus hydrochloric acid
One drop, solution become cloudy.Positive reaction.
2.2.2.2, different hydroxyl oxime ferrosilite reaction: ethanol solution 1ml is taken, 7% hydroxylamine hydrochloride ethanol solution and 10% hydrogen are added
Each 5-6 drop of sodium hydroxide solution sets after heating a few minutes in water-bath, adds 2% hydrochloric acid solution 4-5 drop, adding 1% liquor ferri trichloridi
4-5 drop, solution take on a red color.For positive reaction.
2.2.3, phenolic compound
2.2.3.1、FeCl3Reagent reaction: aqueous adds 1%FeCL3Solution, discovery has dirty-green precipitating after placement.
Positive reaction.
2.2.3.2, one potassium ferricyanide reagent of ferric trichloride reacts: appropriate aqueous, point is on filter paper, after drying,
Spray shows blue spot, positive reaction with one potassium ferricyanide solution of ferric trichloride.
2.2.4, organic acid compound
2.2.4.1, test paper inspection: aqueous test paper inspection, pH is 4.6 compared with standard color version, is positive reaction.
2.2.4.2, phenol indigo plant test solution reaction: appropriate aqueous is smelt, point after drying, is sprayed on filter paper to smell phenol indigo plant second
Alcoholic solution, background yellow spotting blue, positive reaction.
2.2.5, steroidal, triterpene mushroom compound
2.2.5.1, chloroform-strong sulfuric acid response: taking chloroformic solution, and enriching sulfuric acid makes its layering, and the aobvious red of chloroform layer is dense
Sulfuric acid layer has yellow-green fluorescence, positive reaction.
2.2.5.2, trichloroacetic acid reacts: taking ethanol solution appropriate, point after drying, drips trichloroacetic acid second on filter paper
Alcoholic solution is in rufous, positive reaction in 100 degree of bakings in drying box.
Known to verification test result, in extract of the present invention containing flavonoids (a small amount of), Coumarins, interior cruel class,
Phenols, organic acid, steroid and triterpene compound.
Embodiment 3
The external anti esophageal cancer cell that the present embodiment is intended to provide Fructus Trichosanthis extract obtained by a kind of pair of embodiment 1 is real
It tests.
The external anti esophageal cancer activity of tumor cells experiment of Fructus Trichosanthis extract: MTT detection method collects logarithmic growth phase cell,
It is counted with tally, adjustment cell suspension density is (7~8) × 104/ ml, 100 μ are added in every hole in 96 porocyte culture plates
L, each concentration set 3 multiple holes, and volume fraction 5%CO2,37 DEG C of incubations for 24 hours, supernatant are carefully sucked after cell is adherent, is added
100 μ L of culture medium containing 0.00,6.25,12.50,25.00,50.00,100.00,200.00ug/ml containing Fructus Trichosanthis extract, and
If blank control wells.After continuous culture 48h, 20 μ L MTT solution (5g/L) are added in every hole, are continued after cultivating 4h, in careful sucker
Dimethyl sulfoxide 150uL is added in culture medium, every hole, sets low speed on shaking table and is protected from light oscillation 10min, dissolves crystal sufficiently, enzyme
Instrument (Detection wavelength 490nm, reference wavelength 630nm) measurement is marked, and reads each hole absorbance (A) value, calculates and eats by following formula
The proliferation inhibition rate of pipe cancer cell: proliferation inhibition rate=(1- dosing holes A value/blank control wells A value) × 100% calculates simultaneously
Half effective inhibition concentration (IC of the Fructus Trichosanthis extract to esophageal cancer cell50)。
Influence such as Fig. 1-3 institute of the 3.1 various concentration extracts to esophageal cancer cell EC9706, EC109, TE1 cell proliferation
Show.
External inhibitory activity of 3.2 Fructus Trichosanthis extracts to esophageal cancer cell EC9706, EC109, TE-1, IC50Value is such as 1 institute of table
Show:
Table 1
| Cell line | IC50(ug/ml) |
| EC9706 | 3.34 |
| TE-1 | 2.96 |
| EC109 | 15.6 |
Relevant references are as follows:
[1] Ma Baoyu, Geng Yun, Li Yongchao, Yu Delin.The progress of the nearly 10 years domestic anti-tumor Chinese medicine mechanism of action.
Chinese traditional Chinese medicine academic periodical, 2012,30 (11)
[2] Zou Chun ability etc.: the screening and verifying of Fructus Trichosanthis extract antithrombotic drug effect components group.Acta Pharmaceutica Sinica, 2019,54
(3):502-509。
For those skilled in the art, it can be provided various corresponding according to above technical solution and design
Change and modification, and all these change and modification, should be construed as being included within the scope of protection of the claims of the present invention.
Claims (3)
1. Fructus Trichosanthis extract is developing the application in anti esophageal cancer drug.
2. application according to claim 1, which is characterized in that the preparation method of the Fructus Trichosanthis extract includes following step
It is rapid:
S1,75% ethyl alcohol of Snakegourd Fruit is impregnated 24 hours；
S2, it is filtered immediately after being heated to reflux 2 hours, in triplicate, merges liquid phase；
S3, liquid phase obtained by step S2 is concentrated into no ethyl alcohol, ethyl acetate extraction then plus in equal volume；
Ethyl acetate phase is stayed after the completion of S4, extraction, recycling ethyl acetate is evaporated to obtain Fructus Trichosanthis extract.
3. preparation method according to claim 2, which is characterized in that the preparation process of the Snakegourd Fruit in step S1 are as follows: take nine
The beginning of the month is to be sliced mature Snakegourd Fruit, and drying, small fire is fried to golden yellow, then crushed spare.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910695574.8A CN110237115A (en) | 2019-07-30 | 2019-07-30 | The preparation of Fructus Trichosanthis extract and its application in development anti esophageal cancer drug |
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| CN111700896A (en) * | 2020-07-22 | 2020-09-25 | 河南省医药科学研究院 | Application of 3, 29-dibenzoyl trichosanthes seed triol in preparation of anti-esophageal cancer drugs |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103304611A (en) * | 2013-06-18 | 2013-09-18 | 聊城大学 | Method for separating and purifying three flavonoid glycosides from trichosanthes bark |
| CN103342689A (en) * | 2013-06-18 | 2013-10-09 | 聊城大学 | Method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel |
-
2019
- 2019-07-30 CN CN201910695574.8A patent/CN110237115A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103304611A (en) * | 2013-06-18 | 2013-09-18 | 聊城大学 | Method for separating and purifying three flavonoid glycosides from trichosanthes bark |
| CN103342689A (en) * | 2013-06-18 | 2013-10-09 | 聊城大学 | Method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel |
Non-Patent Citations (4)
| Title |
|---|
| 张士舜: "《食管癌中医研治集成》", 31 October 2000, 中国科学技术出版社 * |
| 张穗坚: "《中国地道药材鉴别使用手册 2》", 31 January 2010, 广东旅游出版社 * |
| 李爱峰: ""栝楼（Trichosanthes kirilowii Maxim.）果皮化学成分研究"", 《中国博士学位论文全文数据库 医药卫生科技辑 （月刊 ）》 * |
| 高松: "《辽宁中药志 植物类》", 31 December 2010, 辽宁科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111700896A (en) * | 2020-07-22 | 2020-09-25 | 河南省医药科学研究院 | Application of 3, 29-dibenzoyl trichosanthes seed triol in preparation of anti-esophageal cancer drugs |
| CN111700896B (en) * | 2020-07-22 | 2021-06-29 | 河南省医药科学研究院 | Application of 3, 29-dibenzoyl trichosanthes seed triol in preparation of anti-esophageal cancer drugs |
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