CN110236183A - A method of it being used to prepare the composition of quinoa ferment and its prepares ferment - Google Patents

A method of it being used to prepare the composition of quinoa ferment and its prepares ferment Download PDF

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CN110236183A
CN110236183A CN201910437587.5A CN201910437587A CN110236183A CN 110236183 A CN110236183 A CN 110236183A CN 201910437587 A CN201910437587 A CN 201910437587A CN 110236183 A CN110236183 A CN 110236183A
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parts
quinoa
ferment
extracting solution
fermentation
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雨田
胡一晨
邹亮
赵钢
彭镰心
时小东
郭晓恒
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Chengdu University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/104Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
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  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
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  • Medicines Containing Plant Substances (AREA)
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Abstract

The invention discloses a kind of composition for being used to prepare quinoa ferment and its methods for preparing ferment.The composition includes the component of following weight: 80~100 parts of quinoa, 10~15 parts of hawthorn, 5~10 parts of ginseng, 10~15 parts of fructus lycii, 6~10 parts of Rhizoma Atractylodis Macrocephalae, 10~15 parts of Poria cocos, 15~20 parts of lotus leaf, 10~15 parts of fingered citron, 10~15 parts of honey and 15~20 parts of saccharomycete.The present invention forms compound according to Basic Theories of Chinese Medicine, and there is opposite folk prescription the whole intervention advantage of multiple target point, manifold effect to have better effect in auxiliary adjustment of blood fat and anti-oxidant aspect.

Description

A method of it being used to prepare the composition of quinoa ferment and its prepares ferment
Technical field
The invention belongs to fermentation technical fields, and in particular to a kind of composition for being used to prepare quinoa ferment and its prepare ferment The method of element.
Background technique
Quinoa (Chenopodium quinoa Willd), the entitled quinoa of English, originates in South America andes region, It is print plus the main conventional food of aborigines, has 5000-7000 utilization in more years and plantation history so far.Quinoa is in 20 generation It records the eighties, the space food of astronaut is used for by NASA, FAO (Food and Agriculture Organization of the United Nation) thinks that quinoa is unique single plant Object can meet the food of human body basic nutrition demand, and formal recommendation quinoa is the perfect wholefood of the optimum mankind. Quinoa is a kind of " the full cereal of class " with high nutritive value, not only rich in protein, starch, crude fat and calcium, iron, zinc and Wei Sheng The micronutrients such as plain E, and contain more essential amino acid, such as lysine, histidine and methionine, and quinoa is rich Containing phytochemicals, such as polyphenol, flavones, VB1, folic acid, rutin, Quercetin, isoquercitin, agglutinin.Chenopodiaceae is eaten for a long time Wheat can contribute to reduce blood glucose, blood lipid, blood pressure, while also contain nutriment very abundant in quinoa, such as quinoa polysaccharide Deng.
Ferment (Ferment) is a kind of special complex proteins with catalytic action, and a part is closed by human body itself At a part can be absorbed from food and be obtained.Nowadays most of ferment product had not only included enzyme, but also including its microbes producing cellulase It is the ferment of broad sense with other associated adjustment acting factors, emphasizes its Tiny ecosystem entirety.Edible ferment is one or more new The functional hair that the raw materials such as fresh vegetables, melon and fruit, the medicinal material of integration of drinking and medicinal herbs, edible beneficial bacterium are generated by fermentation process Ferment product, and the wherein multiple nutritional components such as enzyme rich in, minerals, secondary metabolite, vitamin.Largely to function Property enzyme microb studies have shown that ferment rich in the nutrition such as polyphenol, organic acid, amino acid, vitamin, polysaccharide, minerals at Point.In addition, some researches show that fermentation raw materials after microbial fermentation is at ferment, contain multiple efficacies enzyme in the enzyme solution that ferments, mainly Including superoxide dismutase, protease, amylase and lipase etc., ferment is theoretically demonstrated with effect of weight reducing.It removes Weight-reducing is outer, and ferment also has the function of multiple beneficial, can preferably prevent food-borne fatty liver, degrading nitrite, will be extra Waste destruction excludes in vitro at more small molecule;The toxin and cholesterol, blood lipid for excluding to be deposited in blood can be decomposed Etc. harmful substances, reach unimpeded blood vessel, purify blood, promote the circulation of blood and restore the effect of blood vessel elasticity;It can be from cell Level adjusts the vital movement of body, to have anticancer, the good effects of blood pressure lowering;Can also immune function in reinforcement, drop Low blood lipid makes the accumulation of the digested enzyme of gastrointestinal tract, eliminates bodily waste, blood is made to form alkalescent, adjust the intracorporal soda acid of people Balance etc..In addition, ferment also have promote animal metabolism, antifatigue, antiallergy, anti-aging, it is antitumor, antibacterial, relieve the effect of alcohol Protect liver and other effects.
Currently, the ferment product on domestic market has fruits and vegetables fermentoid, Cereals fermentoid, Chinese herbal medicine fermentoid and other classes Ferment, it is more with fruits and vegetables fermentoid product.Using quinoa as primary raw material quinoa ferment phase made of saccharomycete co-fermentation Product is closed to have not been reported.
Summary of the invention
For above-mentioned deficiency in the prior art, the present invention provides a kind of composition for being used to prepare quinoa ferment and its system The method of standby ferment, the quinoa ferment being prepared have excellent lipase activity and antioxidant activity.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is:
A kind of composition being used to prepare quinoa ferment, comprises the following components in parts by weight:
80~100 parts of quinoa, 10~15 parts of hawthorn, 5~10 parts of ginseng, 10~15 parts of fructus lycii, 6~10 parts of Rhizoma Atractylodis Macrocephalae, Poria cocos 10~15 parts, 15~20 parts of lotus leaf, 10~15 parts of fingered citron, 10~15 parts of honey and 15~20 parts of saccharomycete.
Further, it comprises the following components in parts by weight:
100 parts of quinoa, 15 parts of hawthorn, 10 parts of ginseng, 15 parts of fructus lycii, 10 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Poria cocos, 15 parts of lotus leaf, fingered citron 10 parts, 15 parts of honey and 20 parts of saccharomycete.
Using the method for above-mentioned composition preparation quinoa ferment, comprising the following steps:
(1) hawthorn, fructus lycii, Rhizoma Atractylodis Macrocephalae, Poria cocos, lotus leaf and fingered citron are mixed by formula, being crushed to partial size is 100~200 The water that volume is 10~15 times of powder weight is added in mesh, extracts 2~3 times, 2~5h, each extracting solution is mixed every time, must be mentioned Take liquid I;
(2) ginseng is extracted according to step (1) the method, obtains extracting solution II, extracting solution I and extracting solution II is mixed After conjunction, being concentrated into concentration is 1~5g/mL;
(3) primary fermentation: honey is mixed with pretreated quinoa, the saccharomycete after then inoculating activation, and stirring is equal After even after 37~40 DEG C ferment 2~4 days, primary fermentation product is collected;
(4) post-fermentation: the product that step (2) is prepared is added into primary fermentation product again, continues at 35~40 DEG C Fermentation 5~10 days, and collect post-fermentation product;
(5) 6000~7000r/min are centrifuged post-fermentation product, take supernatant.
Further, the volume for the water being added in step (1) is 15 times of powder weight.
Further, extracting solution I and extracting solution II in equal volume after mixing in step (2) are concentrated into concentration as 1g/mL i.e. It can.
Further, in step (3) saccharomycete activation process are as follows:
Aseptically, 20.00mL, the syrup of 0.05g/mL are added into saccharomycete, 40 DEG C of water-bath activation 30min are It can.
Further, in step (3) quinoa preprocessing process are as follows:
The water that volume is 4 times of its weight is added into quinoa and impregnates 10~12h, then removing quinoa surface moisture, and Sterilize 30~40min in steam sterilization pan.
Further, the fermentation temperature of primary fermentation is 37 DEG C in step (3), at first day of primary fermentation, is carried out every 8h Primary stirring is deflated, and beginning in second day carries out primary stirring deflation daily.
Further, the fermentation temperature of post-fermentation is 37 DEG C in step (4).
The ferment that the above method is prepared.
The invention has the benefit that
1, the present invention forms compound according to Basic Theories of Chinese Medicine, and there is opposite folk prescription multiple target point, the whole of manifold effect to intervene Advantage has better effect in auxiliary adjustment of blood fat and anti-oxidant aspect.
It 2, can after being fermented by herbal mixture extracting solution to quinoa added with traditional Chinese medicinal components in inventive formulation Generate a variety of secondary metabolites, and make the obtained quinoa ferment of fermentation that there is Bacterial community abundant, can enhance lipid-loweringing and Anti-oxidation efficacy.
3, inventive formulation is directed to the etiology and pathogenesis of hyperlipidemia deficiency of spleen and stomach, wetness hyperactivity, phlegm resistance, blood stasis, using invigorating the spleen benefit Kidney, clearing dampization are turbid to control its target prescription thinking, wherein ginseng spleen strengthening QI invigorating, and hawthorn resolving sputum disperse accumulation dissipates the stasis of blood, lotus leaf dampness elimination, and tonneau is big The benefits of urine promotees damp evil discharge, and benefit lets out dual-purpose, plays tonifying speen and tonifying kidney, dampness elimination toxin expelling altogether;Poria cocos, Rhizoma Atractylodis Macrocephalae invigorating the spleen for eliminating dampness, fructus lycii tonifying liver The effect of kidney, the qi-regulating of fingered citron soothing liver and strengthening spleen is without impairment of yin, all medicine phases 5, plays strengthening the spleen and stomach and liver and gallbladder, nourishing liver and kidney, turbid resolving sputum altogether.Entirely There is a benefit in side in attacking, giving consideration to both the incidental and fundamental, to reach dissolving stasis resolving phlegm lowering turbidity, the effect of kidney tonifying soothing liver and strengthening spleen, to further enhance quinoa ferment The fat-reducing effect of element, has the function that synergy.
Detailed description of the invention
Fig. 1 is the clearance rate that quinoa ferment removes DPPH free radical;
Fig. 2 is the clearance rate that vitamin C removes DPPH free radical.
Specific embodiment
A specific embodiment of the invention is described below, in order to facilitate understanding by those skilled in the art this hair It is bright, it should be apparent that the present invention is not limited to the ranges of specific embodiment, for those skilled in the art, As long as various change is in the spirit and scope of the present invention that the attached claims limit and determine, these variations are aobvious and easy See, all are using the innovation and creation of present inventive concept in the column of protection.
Embodiment 1
A kind of composition being used to prepare quinoa ferment, comprises the following components in parts by weight:
100 parts of quinoa, 15 parts of hawthorn, 10 parts of ginseng, 15 parts of fructus lycii, 10 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Poria cocos, 15 parts of lotus leaf, fingered citron 10 parts, 15 parts of honey and 20 parts of saccharomycete.
Using the method for above-mentioned composition preparation quinoa ferment, comprising the following steps:
(1) pretreatment of raw material: quinoa 12h is impregnated with the clear water that volume is 4 times of weight of quinoa, removes excess surface water Point, it sterilizes 30 minutes in high-pressure steam sterilizing pan, for use;
(2) actication of culture: aseptically, accurately weighing high activity Angel dried yeast powder 0.20g, 20.00mL be added, The white sand syrup of 0.05g/mL, 40 DEG C of water-baths activate 30min;
(3) medicinal material extract: hawthorn, fructus lycii, Rhizoma Atractylodis Macrocephalae, Poria cocos, lotus leaf and fingered citron are mixed by formula, are crushed to partial size For 100 mesh, the water that volume is 15 times of powder weight is added, extracts twice, 2 hours every time, each extracting solution is mixed, must be extracted Liquid I;Then ginseng is crushed to partial size again is 100 mesh, and the water that volume is 15 times of ginseng powder weight is added, and is extracted twice, often Secondary 2 hours, each extracting solution is mixed, obtains extracting solution II;
(4) it is concentrated: extracting solution I and extracting solution II is mixed in equal volume, and it is 1g/mL that liquid, which is concentrated into concentration, is sterilized, sealing It saves;
(5) primary fermentation: pretreated quinoa is mixed with honey, stirs evenly, then inoculation yeast bacterium, stirs evenly It ferments 4 days in 37 DEG C of fermentations afterwards, first day, every stirring in 8 hours, deflation, it is primary to start stirring daily for second day, and before collection Tunning;
(6) post-fermentation: the product that step (4) is prepared is added in primary fermentation product, mixes thoroughly, continues at 37 DEG C of fermentations 7 days, collect post-fermentation product;
(7) it filters: being centrifuged post-fermentation product 15min under 6500rpm revolving speed, take supernatant to get quinoa zymogen is arrived Liquid.
Embodiment 2
A kind of composition being used to prepare quinoa ferment, comprises the following components in parts by weight:
80 parts of quinoa, 10 parts of hawthorn, 5 parts of ginseng, 10 parts of fructus lycii, 6 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Poria cocos, 15 parts of lotus leaf, fingered citron 10 Part, 10 parts of honey and 15 parts of saccharomycete.
Using the method for above-mentioned composition preparation quinoa ferment, comprising the following steps:
(1) pretreatment of raw material: quinoa 12h is impregnated with the clear water that volume is 4 times of weight of quinoa, removes excess surface water Point, it sterilizes 30 minutes in high-pressure steam sterilizing pan, for use;
(2) actication of culture: aseptically, accurately weighing high activity Angel dried yeast powder 0.20g, 20.00mL be added, The white sand syrup of 0.05g/mL, 40 DEG C of water-baths activate 30min;
(3) medicinal material extract: hawthorn, fructus lycii, Rhizoma Atractylodis Macrocephalae, Poria cocos, lotus leaf and fingered citron are mixed by formula, are crushed to partial size For 200 mesh, the water that volume is 10 times of powder weight is added, extracts twice, 2 hours every time, each extracting solution is mixed, must be extracted Liquid I;Then ginseng is crushed to partial size again is 100 mesh, and the water that volume is 15 times of ginseng powder weight is added, and is extracted twice, often Secondary 2 hours, each extracting solution is mixed, obtains extracting solution II;
(4) it is concentrated: extracting solution I and extracting solution II is mixed in equal volume, and it is 1g/mL that liquid, which is concentrated into concentration, is sterilized, sealing It saves;
(5) primary fermentation: pretreated quinoa is mixed with honey, stirs evenly, then inoculation yeast bacterium, stirs evenly It ferments 4 days in 37 DEG C of fermentations afterwards, first day, every stirring in 8 hours, deflation, it is primary to start stirring daily for second day, and before collection Tunning;
(6) post-fermentation: the product that step (4) is prepared is added in primary fermentation product, mixes thoroughly, continues at 37 DEG C of fermentations 7 days, collect post-fermentation product;
(7) it filters: being centrifuged post-fermentation product 15min under 6500rpm revolving speed, take supernatant to get quinoa zymogen is arrived Liquid.
Embodiment 3
A kind of composition being used to prepare quinoa ferment, comprises the following components in parts by weight:
85 parts of quinoa, 12 parts of hawthorn, 8 parts of ginseng, 15 parts of fructus lycii, 10 parts of Rhizoma Atractylodis Macrocephalae, 12 parts of Poria cocos, 18 parts of lotus leaf, fingered citron 15 Part, 11 parts of honey and 16 parts of saccharomycete.
Using the method for above-mentioned composition preparation quinoa ferment, comprising the following steps:
(1) pretreatment of raw material: quinoa 12h is impregnated with the clear water that volume is 4 times of weight of quinoa, removes excess surface water Point, it sterilizes 30 minutes in high-pressure steam sterilizing pan, for use;
(2) actication of culture: aseptically, accurately weighing high activity Angel dried yeast powder 0.20g, 20.00mL be added, The white sand syrup of 0.05g/mL, 40 DEG C of water-baths activate 30min;
(3) medicinal material extract: hawthorn, fructus lycii, Rhizoma Atractylodis Macrocephalae, Poria cocos, lotus leaf and fingered citron are mixed by formula, are crushed to partial size For 100 mesh, the water that volume is 10 times of powder weight is added, extracts twice, 2 hours every time, each extracting solution is mixed, must be extracted Liquid I;Then ginseng is crushed to partial size again is 100 mesh, and the water that volume is 15 times of ginseng powder weight is added, and is extracted twice, often Secondary 2 hours, each extracting solution is mixed, obtains extracting solution II;
(4) it is concentrated: extracting solution I and extracting solution II is mixed in equal volume, and it is 1g/mL that liquid, which is concentrated into concentration, is sterilized, sealing It saves;
(5) primary fermentation: pretreated quinoa is mixed with honey, stirs evenly, then inoculation yeast bacterium, stirs evenly It ferments 4 days in 37 DEG C of fermentations afterwards, first day, every stirring in 8 hours, deflation, it is primary to start stirring daily for second day, and before collection Tunning;
(6) post-fermentation: the product that step (4) is prepared is added in primary fermentation product, mixes thoroughly, continues at 37 DEG C of fermentations 7 days, collect post-fermentation product;
(7) it filters: being centrifuged post-fermentation product 15min under 6500rpm revolving speed, take supernatant to get quinoa zymogen is arrived Liquid.
Comparative example 1
It is fermented with 85 parts of quinoa, 15 parts of honey and 15 parts of saccharomycete, obtains the simple Chenopodiaceae without Chinese medicine component Wheat ferment.
Experimental example
1, lipase activity and the anti-oxidant work in the quinoa ferment that Examples 1 to 3 and comparative example 1 are prepared are detected Property, the result is shown in tables 1.
1 quinoa ferment biological activity of table
According to 1 data of table it is found that the quinoa ferment that Examples 1 to 3 is prepared is substantially better than comparative example 1, wherein especially with The effect of quinoa ferment that embodiment 1 is prepared, is best.
2, using vitamin C as positive control, compare the reducing power for the quinoa ferment that the embodiment of the present invention 1 is prepared, The result is shown in Figure 1 and Fig. 2, wherein Fig. 1 is that quinoa ferment removes DPPH free radical result;Fig. 2 is that vitamin C removes DPPH freedom Base junction fruit.
According to Fig. 1 and Fig. 2 it is found that the linear equation that quinoa ferment working solution removes DPPH free radical is y= The amount of 0.0668x-0.946, R=0.9990, DPPH free radical scavenging activity and quinoa ferment working solution is in good linear relationship; The linear equation that vitamin C working solution removes DPPH free radical is y=0.2449x-3.456, and R=0.9967, DPPH are certainly It is in good linear relationship by base clearance rate and the amount of vitamin C working solution.Finally by formula, when clearance rate is 50%, 21.65 μ L quinoa ferment enzyme liquids are that the oxidation resistance of vitamin c solution of 1mg/mL is suitable with 16.15 μ L concentration, i.e. Chenopodiaceae The reducing power of wheat enzyme liquid is equivalent to the 75% of vitamin c solution.
It is indicated above that can just be prepared only under the cooperation of inventive formulation and preparation process with excellent fat The quinoa ferment of enzyme activity and antioxidant activity.

Claims (10)

1. a kind of composition for being used to prepare quinoa ferment, which is characterized in that comprise the following components in parts by weight:
80~100 parts of quinoa, 10~15 parts of hawthorn, 5~10 parts of ginseng, 10~15 parts of fructus lycii, 6~10 parts of Rhizoma Atractylodis Macrocephalae, Poria cocos 10~ 15 parts, 15~20 parts of lotus leaf, 10~15 parts of fingered citron, 10~15 parts of honey and 15~20 parts of saccharomycete.
2. the composition according to claim 1 for being used to prepare quinoa ferment, which is characterized in that including following parts by weight Component:
100 parts of quinoa, 15 parts of hawthorn, 10 parts of ginseng, 15 parts of fructus lycii, 10 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Poria cocos, 15 parts of lotus leaf, 10 parts of fingered citron, 15 parts of honey and 20 parts of saccharomycete.
3. using the method for composition of any of claims 1 or 2 preparation quinoa ferment, which comprises the following steps:
(1) hawthorn, fructus lycii, Rhizoma Atractylodis Macrocephalae, Poria cocos, lotus leaf and fingered citron are mixed by formula, being crushed to partial size is 100~200 mesh, The water that volume is 10~15 times of powder weight is added, extracts 2~3 times, 2~5h, each extracting solution is mixed, extracting solution is obtained every time Ⅰ;
(2) ginseng is extracted according to step (1) the method, obtains extracting solution II, extracting solution I and extracting solution II is mixed Afterwards, being concentrated into concentration is 1~5g/mL;
(3) primary fermentation: honey is mixed with pretreated quinoa, the saccharomycete after then inoculating activation, after mixing evenly After 37~40 DEG C ferment 2~4 days, primary fermentation product is collected;
(4) post-fermentation: the product that step (2) is prepared being added into primary fermentation product again, continues at 35~40 DEG C of fermentations 5 ~10 days, and collect post-fermentation product;
(5) 6000~7000r/min are centrifuged post-fermentation product, take supernatant.
4. according to the method described in claim 3, it is characterized in that, the volume for the water being added in step (1) is powder weight 15 times.
5. according to the method described in claim 3, it is characterized in that, extracting solution I described in step (2) and extracting solution II are isometric After mixing, being concentrated into concentration is 1g/mL.
6. according to the method described in claim 3, it is characterized in that, the activation process of saccharomycete described in step (3) are as follows:
Aseptically, 20.00mL, the syrup of 0.05g/mL are added into saccharomycete, 40 DEG C of water-baths activate 30min.
7. according to the method described in claim 3, it is characterized in that, the preprocessing process of quinoa described in step (3) are as follows:
The water that volume is 4 times of its weight is added into quinoa and impregnates 10~12h, then removes quinoa surface moisture, and in steam Sterilize 30~40min in autoclave.
8. according to the method described in claim 3, it is characterized in that, the fermentation temperature of primary fermentation described in step (3) be 37 DEG C, At first day of primary fermentation, primary stirring was carried out every 8h and is deflated, beginning in second day carries out primary stirring deflation daily.
9. according to the method described in claim 3, it is characterized in that, the fermentation temperature of post-fermentation described in step (4) is 37 DEG C.
10. the ferment that any one of claim 3~9 the method is prepared.
CN201910437587.5A 2019-05-24 2019-05-24 A method of it being used to prepare the composition of quinoa ferment and its prepares ferment Pending CN110236183A (en)

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CN113508904A (en) * 2021-05-26 2021-10-19 扬州大学 Preparation method of compound fermentation liquor with lipid-lowering function

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