CN110231388A - A kind of preparation of chitosan/CT-DNA Modified Gold Electrode and its method for electrochemical recognition Tryptophan enantiomer - Google Patents
A kind of preparation of chitosan/CT-DNA Modified Gold Electrode and its method for electrochemical recognition Tryptophan enantiomer Download PDFInfo
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- CN110231388A CN110231388A CN201910623356.3A CN201910623356A CN110231388A CN 110231388 A CN110231388 A CN 110231388A CN 201910623356 A CN201910623356 A CN 201910623356A CN 110231388 A CN110231388 A CN 110231388A
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- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
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Abstract
The present invention relates to a kind of preparation of chitosan/CT-DNA Modified Gold Electrode and its for the method for electrochemical recognition Tryptophan enantiomer, the following steps are included: being first prepared into chitosan-modified electrode, chitosan-modified electrode is placed in DNA solution again and is further modified, it is prepared into chitosan/CT-DNA Modified Gold Electrode, is used for electrochemical recognition Tryptophan enantiomer.Effect of the invention are as follows: chitosan/CT-DNA Modified Gold Electrode preparation process is simple, and environmental protection, at low cost and modified electrode tryptophan enantiomer has apparent recognition effect.
Description
Technical field
The present invention relates to a kind of preparation of chitosan/CT-DNA Modified Gold Electrode and its it is used for electrochemical recognition color ammonia
The method of sour enantiomer, belongs to electrochemical analysis and field of biotechnology.
Background technique
Most of amino acid all have enantioselectivity in life system.The chiral molecules of various configuration is in toxicity, biology
There are significant differences for chemical activity, transmission and metabolic mechanism etc..Therefore, chiral Recognition is always chemistry and biology research
Hot spot.The deficiencies of that there is sensitivity is low for existing some spectra methods, complicated for operation, and analysis cost is high, so finding one
The analysis method that kind has good prospect is vital.Electrochemical analysis method has that high sensitivity, response time be fast, behaviour
Make simplicity, and the advantages that distinguishing different aminoacids enantiomer can be reached by regulating and controlling the chiral interface of modified electrode, therefore
Electrochemical Detection based on chiral Recognition is with good application prospect.
Summary of the invention
In the present invention, using the hydrogen bond action of chitosan and DNA, by chitosan and DNA
Self assembly forms a film on glass-carbon electrode, thus by LBL self-assembly in conjunction with electrochemical techniques.Chitosan is a kind of with biology
Compatibility, biodegradability, the natural polysaccharide for possessing chiral environment, and DNA itself also has intrinsic chirality
The two is combined as the identification material of chiral amino acid by structure.
A kind of chitosan/CT-DNA Modified Gold Electrode preparation side for electrochemical recognition Tryptophan enantiomer
Method, comprising the following steps:
A, it prepares chitosan-modified electrode: preparing chitosan solution, glass-carbon electrode is immersed in chitosan solution, impregnate one
It fixes time, obtains chitosan-modified electrode；
B, it prepares chitosan/CT-DNA Modified Gold Electrode: DNA solution is prepared, by chitosan-modified electricity
Certain time in DNA solution is immersed after washing with water in pole, takes out room temperature and dries, obtains chitosan/deoxyribose core
Sour modified electrode；
C, electrochemical recognition Tryptophan enantiomer: chitosan/CT-DNA Modified Gold Electrode is working electrode, Ag/
AgCl electrode is reference electrode, and platinum electrode is to immerse the three-electrode system in the L-/D- tryptophan solution prepared to electrode
(in L-Trp or D-trp solution) is incubated for after a certain period of time, carries out test test using pulse voltammetry.
Further, chitosan concentration is 1~3mg/mL in step a, and soaking time is 20~60min.
Further, the concentration of DNA is 1~3mg/mL in step b, and soaking time is 10~30min.
Further, the concentration of L-/D- tryptophan solution is 0.05~0.8mM in step c, and scanning current potential is 0.4~1V,
Incubation time is 1~15min.
What the present invention obtained has the beneficial effect that
The present invention knowledge of self assembly film forming as chiral amino acid on glass-carbon electrode by chitosan and DNA
Other material, the composite film material modified electrode is compared with single chitosan or CT-DNA Modified Gold Electrode, tryptophan mapping
The recognition effect of body significantly improves, and the preparation method is cheap, environmental protection, simply.
Detailed description of the invention
Fig. 1 is chitosan/DNA composite membrane scanning electron microscope (SEM) photograph.
Fig. 2 is that bare glassy carbon electrode schemes the DPV that L-/D- Tryptophan enantiomer identifies.
Fig. 3 is that chitosan-modified glass-carbon electrode schemes the DPV that L-/D- Tryptophan enantiomer identifies.
Fig. 4 is that DNA modified glassy carbon electrode schemes the DPV that L-/D- Tryptophan enantiomer identifies.
Fig. 5 is that chitosan/DNA modified glassy carbon electrode schemes the DPV that L-/D- Tryptophan enantiomer identifies.
Fig. 6 be chitosan/DNA modified glassy carbon electrode measure in mixed solution different L-Trp contents with
The relational graph of electric current.
Specific embodiment
Presently in connection with specific implementation the present invention will be further described, following embodiment be intended to illustrate invention rather than it is right
The present invention further limits.
Embodiment one:
Chitosan/DNA film is prepared to characterize for scanning electron microscope
A, 50mg chitosan is weighed, it is molten to be dissolved in obtained 1mg/mL chitosan in 1% acetum of 50mL (volume ratio)
Liquid.
B, it weighs 50mg DNA (purchased from aladdin) and is dissolved in 50mL deionized water obtained 1mg/mL deoxidation
Rna solution.
C, silicon wafer is cleaned and is used and is dried with nitrogen, is placed in the chitosan solution of 1mg/mL, impregnated 15min and obtain chitosan
Modify silicon wafer
D, the deoxidation core containing 1mg/mL is immersed after cleaning chitosan-modified silicon wafer obtained in step c with deionized water
In ribosomal ribonucleic acid solution, impregnates 15min and obtain chitosan/DNA film.
Embodiment two:
Chitosan-modified electrode, CT-DNA Modified Gold Electrode, chitosan/DNA modification glass are prepared respectively
Carbon electrode is used for electrochemical recognition Tryptophan enantiomer.
Steps are as follows:
It prepares chitosan-modified electrode: preparing 4mL 1mg/mL chitosan solution, glass-carbon electrode is immersed into chitosan solution
In, 30min is impregnated, chitosan-modified glass-carbon electrode is obtained.
It prepares CT-DNA Modified Gold Electrode: preparing 1mg/mL DNA solution, glass-carbon electrode is immersed into shell
In glycan solution, 30min is impregnated, obtains the glass-carbon electrode of DNA modification.
Electrochemistry chiral Recognition of the chitosan/CT-DNA Modified Gold Electrode for Tryptophan enantiomer is prepared, will be made
The chitosan-modified electrode obtained immerses in the DNA solution containing 1mg/mL after being cleaned with deionized water, impregnates
15min obtains chitosan/DNA modification glass-carbon electrode.
The modified electrode of preparation is used for the electrochemistry chiral Recognition of Tryptophan enantiomer respectively.
It is respectively working electrode by the modified electrode of above-mentioned preparation, Ag/AgCl electrode is reference electrode, and platinum electrode is pair
The three-electrode system is immersed in 10mL and contains 0.05mM L-Trp or pH=7 containing 0.05mMD- tryptophan by electrode
In PBS solution, after being incubated for 1min.DPV test is carried out in the potential window of 0.4~1V, compares electric current difference.
Naked glass carbon modified electrode D-/L- tryptophan electric current ratio at current potential 0.76V is 1 as shown in Figure 2；
Chitosan-modified glass-carbon electrode D-/L- tryptophan electric current ratio at current potential 0.74V is 1.4 as shown in Figure 3；
DNA modified glassy carbon electrode D-/L- tryptophan electric current ratio at 0.74V is 1.64 as shown in Figure 4；
Chitosan/DNA modified glassy carbon electrode D-/L- tryptophan electric current ratio at 0.76V is as shown in Figure 5
4.02, illustrate that chitosan/DNA modified glassy carbon electrode recognition efficiency significantly improves.
Embodiment three:
Prepare chitosan/CT-DNA Modified Gold Electrode step such as example two.It is working electrode by the electrode of preparation,
Ag/AgCl electrode is reference electrode, and platinum electrode is that three-electrode system is immersed in 10mL respectively and contains different L- colors to electrode
In the PBS solution of the mixed proportion of propylhomoserin and D-trp.DPV test is carried out in the potential window of 0.4~1V, obtains electric current
With L-Trp content change diagram.As shown in Figure 6: electric current and L-Trp content are in a linear relationship.
Beneficial effects of the present invention: chitosan/CT-DNA Modified Gold Electrode preparation method is cheap, environmental protection, simply,
And the enantiomer of the modified electrode tryptophan has significant recognition effect.
Claims (5)
1. a kind of chitosan/CT-DNA Modified Gold Electrode preparation method for electrochemical recognition Tryptophan enantiomer,
It is characterized in that, steps are as follows:
A, it prepares chitosan-modified electrode: preparing chitosan solution, glass-carbon electrode is immersed in chitosan solution, impregnate a timing
Between, obtain chitosan-modified electrode；
B, it prepares chitosan/CT-DNA Modified Gold Electrode: preparing DNA solution, the step a shell prepared is gathered
Be immersed in certain time in DNA solution after the cleaning of sugar-modified electrode water, take out room temperature and dry, obtain chitosan/
CT-DNA Modified Gold Electrode；
C, electrochemical recognition Tryptophan enantiomer: using chitosan/CT-DNA Modified Gold Electrode as working electrode, Ag/AgCl
Electrode is reference electrode, and platinum electrode is that the three-electrode system is immersed to the L-/D- tryptophan solution of preparation simultaneously to electrode
In, it is incubated for after a certain period of time, is tested using pulse voltammetry.
2. according to claim 1 for the chitosan of electrochemical recognition Tryptophan enantiomer/DNA modification electricity
The preparation method of pole, it is characterised in that: in the step a concentration of chitosan solution be 1~3mg/mL, soaking time be 20~
60min。
3. according to claim 1 for the chitosan of electrochemical recognition Tryptophan enantiomer/DNA modification electricity
The preparation method of pole, it is characterised in that: DNA solution concentration is 1~3mg/mL in the step b, and soaking time is
10~30min.
4. according to claim 1 for the chitosan of electrochemical recognition Tryptophan enantiomer/DNA modification electricity
The preparation method of pole, it is characterised in that: the concentration of L-/D- tryptophan is 0.05~0.8mM in the step c.
5. according to claim 1 for the chitosan of electrochemical recognition Tryptophan enantiomer/DNA modification electricity
The preparation method of pole, it is characterised in that: it is 0.4~1.0V that current potential is scanned in the step c, and incubation time is 1~15min.
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| CN201910361752.3A CN109975379A (en) | 2019-04-30 | 2019-04-30 | A kind of chitosan/CT-DNA Modified Gold Electrode preparation method for electrochemical recognition Tryptophan enantiomer |
| CN2019103617523 | 2019-04-30 |
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| CN201910623356.3A Pending CN110231388A (en) | 2019-04-30 | 2019-07-11 | A kind of preparation of chitosan/CT-DNA Modified Gold Electrode and its method for electrochemical recognition Tryptophan enantiomer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111175359A (en) * | 2020-01-17 | 2020-05-19 | 常州大学 | Chitosan/polyacrylic acid multilayer film modified electrode for electrochemical recognition of tryptophan enantiomer and preparation method thereof |
| CN112595769A (en) * | 2020-12-07 | 2021-04-02 | 常州大学 | Method for amplifying and identifying tryptophan enantiomer by using PEI-Fc in-situ generated nano silver |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110243897B (en) * | 2019-07-10 | 2021-03-23 | 常州大学 | Selective recognition of tryptophan enantiomer by polyethyleneimine/chiral peptide modified electrode |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103411889A (en) * | 2013-08-21 | 2013-11-27 | 江南大学 | Method for detecting endonuclease DNaseI activity based on chiral gold tetrahedral nanostructure |
| CN104897596A (en) * | 2015-05-25 | 2015-09-09 | 东华大学 | Method for detecting chiral compound based on aptamer modified nanogold |
| CN105758914A (en) * | 2016-02-29 | 2016-07-13 | 常州大学 | Preparation and chiral-recognition tryptophan based on sulfonated chitosan/beta-cyclodextrin chiral sensor |
| CN106908507A (en) * | 2017-02-22 | 2017-06-30 | 常州大学 | A kind of preparation and its application of phenylalanine dipeptide Chitosan Composites |
-
2019
- 2019-04-30 CN CN201910361752.3A patent/CN109975379A/en not_active Withdrawn
- 2019-07-11 CN CN201910623356.3A patent/CN110231388A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103411889A (en) * | 2013-08-21 | 2013-11-27 | 江南大学 | Method for detecting endonuclease DNaseI activity based on chiral gold tetrahedral nanostructure |
| CN104897596A (en) * | 2015-05-25 | 2015-09-09 | 东华大学 | Method for detecting chiral compound based on aptamer modified nanogold |
| CN105758914A (en) * | 2016-02-29 | 2016-07-13 | 常州大学 | Preparation and chiral-recognition tryptophan based on sulfonated chitosan/beta-cyclodextrin chiral sensor |
| CN106908507A (en) * | 2017-02-22 | 2017-06-30 | 常州大学 | A kind of preparation and its application of phenylalanine dipeptide Chitosan Composites |
Non-Patent Citations (2)
| Title |
|---|
| XIAOGANG GU ET.AL: "DNA-Inspired Electrochemical Recognition of Tryptophan Isomers by Electrodeposited Chitosan and Sulfonated Chitosan", 《ANAL. CHEM.》 * |
| 郭丽菊: "基于石墨烯纳米复合材料的电化学手性传感器研究", 《中国优秀硕士学位论文全文数据库（电子期刊） 工程科技I 辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111175359A (en) * | 2020-01-17 | 2020-05-19 | 常州大学 | Chitosan/polyacrylic acid multilayer film modified electrode for electrochemical recognition of tryptophan enantiomer and preparation method thereof |
| CN111175359B (en) * | 2020-01-17 | 2022-11-01 | 常州大学 | Chitosan/polyacrylic acid multilayer film modified electrode for electrochemical recognition of tryptophan enantiomer and preparation method thereof |
| CN112595769A (en) * | 2020-12-07 | 2021-04-02 | 常州大学 | Method for amplifying and identifying tryptophan enantiomer by using PEI-Fc in-situ generated nano silver |
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