CN110218761A - Utilize the method for husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity - Google Patents
Utilize the method for husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity Download PDFInfo
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- 230000007198 pollen germination Effects 0.000 title claims abstract description 47
- 238000000338 in vitro Methods 0.000 title claims abstract description 43
- 235000014787 Vitis vinifera Nutrition 0.000 title claims abstract description 40
- 240000006365 Vitis vinifera Species 0.000 title claims abstract description 40
- 235000012907 honey Nutrition 0.000 title claims abstract description 40
- 230000000694 effects Effects 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000004576 sand Substances 0.000 title claims abstract description 25
- 238000003556 assay Methods 0.000 title claims abstract description 9
- 238000009630 liquid culture Methods 0.000 title claims abstract description 9
- 229940046536 tree pollen allergenic extract Drugs 0.000 title claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims description 16
- 230000000007 visual effect Effects 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 7
- 230000006378 damage Effects 0.000 claims description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004327 boric acid Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000012549 training Methods 0.000 claims 1
- 241001180182 Calligonum Species 0.000 abstract description 10
- 238000011160 research Methods 0.000 abstract description 4
- 238000009402 cross-breeding Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 230000001850 reproductive effect Effects 0.000 abstract description 2
- 239000011575 calcium Substances 0.000 description 5
- 238000005034 decoration Methods 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 230000010152 pollination Effects 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 241000219050 Polygonaceae Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- UWSONZCNXUSTKW-UHFFFAOYSA-N 4,5-Dimethylthiazole Chemical compound CC=1N=CSC=1C UWSONZCNXUSTKW-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001371568 Calligonum ebinuricum Species 0.000 description 1
- 241000282836 Camelus dromedarius Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 208000036119 Frailty Diseases 0.000 description 1
- DKNPRRRKHAEUMW-UHFFFAOYSA-N Iodine aqueous Chemical compound [K+].I[I-]I DKNPRRRKHAEUMW-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 150000003536 tetrazoles Chemical class 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5097—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving plant cells
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Abstract
The present invention relates to a kind of methods using husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity, this method is completed using preparation, pollen collection, in-vitro pollen germination and the pollen tube growth of culture medium and the determination step of pollen activity, the pollen that the method for the invention chooses Jinghe sand honey raisin tree carries out in-vitro pollen culture, on the basis of determining husky honey raisin tree in-vitro pollen germination cultivating system, can reliably, effectively measure pollen activity.The index studied using pollen germination rate as pollen activity (pollen tube length is longer than pollen diameter and is considered as sprouting).A kind of simple, quick, feasible method is provided for Calligonum plant pollen vitality test, provides basis for the crossbreeding of the category and reproductive biology research.
Description
Technical field
The present invention relates to a kind of sides using husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity
Method.
Background technique
Polygonaceae (Polygonaceae) Calligonum (Calligonum L.) shrub is extensive on arid biogeographic zone desert and gobi
The strong non-irrigated life of distribution or extreme xerophyte, it is mainly distributed on the Sahara, Mediterranean, the Soviet Union Central Asia and Kazakhstan and Asia
Middle part.About 35 kinds altogether of the category, China has 23 kinds, is mainly distributed on the areas such as Xinjiang, the Inner Mongol, Gansu, Qinghai, and wherein Xinjiang is most
It is more, there are 22 kinds.Calligonum plant is sandy and one of the important constructive species of part chomoeremion vegetation, its drought-resistant, wind resistance
Erosion sand is buried, is grown fastly, is easy to breed, because of its excellent ability of checking winds and fixing drifting sand, in terms of being widely used in ecological construction always.
Current year of Calligonum plant, raw dry, fresh sprout was the good feed of camel and sheep, at the same have fuel wood, fix the sand,
The economic values such as nectar source, so that the Calligonum plant of NATURAL DISTRIBUTION is destroyed.By our investigations of team for years,
It was found that the distribution site recorded on many original samples has been difficult to search out trace (Jeminay in such as Xinjiang, five of husky honey raisin tree
The ground such as platform, Bole), existing population is in addition to other than the artificial destruction of desert depths is relatively light, in many regions all due to herding, opening up wasteland
The reasons such as soil, exploitation tourism, new highway are by different degrees of destruction.On the other hand, because being grown in husky honey raisin tree dry more
Non-irrigated desert belt, the natural conditions that dust storm is big, hydrologic condition is poor, soil depletion and more salination etc. are severe make plant be difficult to shape
At structure is complicated, closing and rich in multifarious group, so that structure of community is very fragile, and human factor exacerbates habitat
Fragmentation.
Therefore, the in situ conservation of Calligonum plant, popularization and application are just particularly important, how in situ conservation process
Middle holding genetic diversity, if obtaining more seeds in sexual reproduction process, how to be increased by artificial pollination it is solid,
This requires the feature for understanding pollen activity, preferably serves breeding and popularization.
The more of the measuring method of pollen activity is artificial field pollination method, decoration method and germination in vitro at present
Method.Artificial field pollinating method needs to acquire pollen, using the method for artificial pollination, counts setting percentage to count pollen germination
Rate, this method is direct, reliable, but time-consuming and laborious, while being influenced and being restricted by season and weather.Decoration method be by using
Coloring agent dyes pollen grain, and the height of pollen activity is judged according to pollen color change.Common staining reagent has chlorine
Change triphen tetrazole (TTC), 3- (4,5- dimethylthiazole -2) -2,5- diphenyltetrazolium bromide bromide (MTT) and iodine-potassium iodide
(I2- KI) solution etc..We have found in Calligonum plant by dyeing fresh and pollen that is burning to death respectively, with this three
The result of kind coloring agent is less desirable, cannot accurately identify very much pollen activity.I2- KI coloring is deeper, cannot distinguish well
The power of other pollen activity, it might even be possible to not having great-hearted husky honey raisin tree pollen staining into imperial purple；After the dyeing of TTC method,
Colouring discrimination is little, can not define vibrant and debility well；MTT decoration method accuracy rate is high, but reagent price is expensive, examination
Agent preservation condition is more harsh, and qualitative change once occurs for reagent, will seriously affect experimental result, needs to be rapidly completed experiment.Phase
Than for, in-vitro pollen culture observation pollen tube growth result is more stable, can compare the reality for being truly reflected pollen activity
Border situation, and in-vitro pollen germination reproducibility of results is high, it is easy to operate.
Summary of the invention
Husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree is utilized the object of the present invention is to provide a kind of
The method of powder vigor, this method are living using preparation, pollen collection, in-vitro pollen germination and the pollen tube growth and pollen of culture medium
The determination step of power is completed, and the pollen that the method for the invention chooses Jinghe sand honey raisin tree carries out in-vitro pollen culture, husky determining
On the basis of honey raisin tree in-vitro pollen germination cultivating system, can reliably, effectively measure pollen activity.Using pollen germination rate as
The index of pollen activity research (pollen tube length is longer than pollen diameter and is considered as sprouting).For Calligonum plant pollen vitality test
A kind of simple, quick, feasible method is provided, provides basis for the crossbreeding of the category and reproductive biology research.
The husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity of a kind of utilization of the present invention
Method follows these steps to carry out:
The preparation of culture medium:
A, using distilled water as solvent, pH value 5.6,100g/L sucrose, 32mg/L boric acid, 36.5mg/L Ca (NO3)2, light
According to 20-35min after culture；
Pollen collection:
B, selection growth and development is good, the husky honey raisin tree plant of stalwartness of no disease and pests harm, in full-bloom stage morning time 8:00-9:00
Entire spray is acquired, is taken back, is inserted in bottle, naturally open to flower, pollen sheds, and carefully takes flower with tweezers, collects standby
With；
In-vitro pollen germination and pollen tube growth:
C, the configured culture medium of step a is instilled in the groove of concave glass slide, dips the flower that step b is obtained with writing brush
Powder is uniformly trickled down on liquid medium within, is placed in bottom and is lined in the culture dish of filter paper, covers lid and be placed on constant incubator
Interior, culture, in vitro culture time 25-35min are sprouted in 20-30 DEG C of temperature in vitro illumination；
The measurement of pollen activity:
D, be more than pollen grain diameter to pollen tube length it is pollen germination by after step c in vitro culture 25-35min, starts
Pollen germination rate is counted, with the micro- sem observation pollen germination situation of OLYMPUS BX51 type, each processing is repeated 3 times, Mei Gechong
Multiple 3 visuals field of random observation, each visual field pollen frequence are greater than 50, and statistics pollen germination number accounts for the ratio of pollen frequence, meter
Calculate pollen activity.
The husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity of a kind of utilization of the present invention
Method, the factor of in-vitro pollen culture most critical is to select suitable culture medium and condition of culture in this method, common to train
The basis for supporting base is sugar, boric acid and calcium.Type, concentration and the temperature for the culture medium that the pollen germination of different plants needs
Deng all difference, under the conditions of suitable, sprouting that pollen germination could be relatively good.
The husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity of a kind of utilization of the present invention
Method, selection carry out experimental study in Chinese Academy of Sciences's Turpan Desert Botanical Garden.Husky honey raisin tree abundant is had collected in botanical garden
Germ plasm resource, has introduced a fine variety in garden and had planted for more than 40 years, and growth adaptation is good.By in the more of Turpan Desert Botanical Garden
It is secondary to repeat to test: to collect pollen, pollen is placed in culture solution and carries out in vitro culture, observes pollen germination under the microscope
Situation counts the experiment of pollen germination rate, the results showed that on the basis of determining husky honey raisin tree in-vitro pollen germination cultivating system,
Can reliably, effectively measure pollen activity.The crossbreeding of the Calligonum plant under in situ conservation and breeding are given birth to simultaneously
The research of object provides more experimental datas and support.
Detailed description of the invention
Fig. 1 is in-vitro pollen culture 10min of the present invention figure；
Fig. 2 is in-vitro pollen culture 60min of the present invention figure.
Specific embodiment
Embodiment 1 (by taking Jinghe sand honey raisin tree as an example)
The preparation of culture medium:
A, using distilled water as solvent, pH value 5.6,100g/L sucrose, 32mg/L boric acid, 36.5mg/L Ca (NO3)2, light
According to 20min after culture；
Pollen collection:
B, selection growth and development is good, healthy and strong Jinghe sand honey raisin tree plant of no disease and pests harm, in full-bloom stage morning time 8:00-
9:00 acquires entire spray, takes back, inserts in bottle, naturally open to flower, and pollen sheds, and carefully takes flower with tweezers, receives
Collect spare；
In-vitro pollen germination and pollen tube growth:
C, the configured culture medium of step a is instilled in the groove of concave glass slide, dips the flower that step b is obtained with writing brush
Powder is uniformly trickled down on liquid medium within, is placed in bottom and is lined in the culture dish of filter paper, covers lid and be placed on constant incubator
Interior, culture, in vitro culture time 25min are sprouted in 20 DEG C of temperature in vitro illumination；
The measurement of pollen activity:
D, by after step c in vitro culture 25min, pollen germination is thought more than pollen grain diameter to pollen tube length, i.e.,
It can start to count pollen germination rate, with the micro- sem observation pollen germination situation of OLYMPUS BX51 type, each processing is repeated 3 times,
Each to repeat 3 visuals field of random observation, each visual field pollen frequence is greater than 50, and statistics pollen germination number accounts for the ratio of pollen frequence
Example calculates pollen activity；The pollen activity of measurement Jinghe sand honey raisin tree difference loose powder time, investigation loose powder time and pollen activity
Relationship is shown in Table 1:
The pollen activity variation of 1 Jinghe sand honey raisin tree (C.ebinuricum) of table different loose powder times
| The loose powder time (h) | Pollen activity (%) |
| 2h | 94.90 |
| 4h | 88.32 |
| 6h | 82.32 |
| 8h | 77.88 |
| 10h | 70.32 |
| 12h | 66.24 |
| 16h | 57.47 |
| 20h | 52.37 |
| 24h | 41.75 |
| 28h | 38.42 |
| 32h | 33.83 |
| 36h | 27.68 |
Table 1 is shown: the pollen activity variation tendency of different loose powder loose powder time, Jinghe time sand honey raisin trees.
Embodiment 2 (by taking Jinghe sand honey raisin tree as an example)
The preparation of culture medium:
A, using distilled water as solvent, pH value 5.6,100g/L sucrose, 32mg/L boric acid, 36.5mg/L Ca (NO3)2, light
According to 30min after culture；
Pollen collection:
B, selection growth and development is good, healthy and strong Jinghe sand honey raisin tree plant of no disease and pests harm, in full-bloom stage morning time 8:00-
9:00 acquires entire spray, takes back, inserts in bottle, naturally open to flower, and pollen sheds, and carefully takes flower with tweezers, receives
Collect spare；
In-vitro pollen germination and pollen tube growth:
C, the configured culture medium of step a is instilled in the groove of concave glass slide, dips the flower that step b is obtained with writing brush
Powder is uniformly trickled down on liquid medium within, is placed in bottom and is lined in the culture dish of filter paper, covers lid and be placed on constant incubator
Interior, culture, in vitro culture time 30min are sprouted in 30 DEG C of temperature in vitro illumination；
The measurement of pollen activity:
D, by after step c in vitro culture 30min, pollen germination is thought more than pollen grain diameter to pollen tube length, i.e.,
It can start to count pollen germination rate, with the micro- sem observation pollen germination situation of OLYMPUS BX51 type, each processing is repeated 3 times,
Each to repeat 3 visuals field of random observation, each visual field pollen frequence is greater than 50, and statistics pollen germination number accounts for the ratio of pollen frequence
Example calculates pollen activity；And the pollen activity of Jinghe sand honey raisin tree difference loose powder time is measured, investigate loose powder time and pollen activity
Relationship be shown in Table 1.
Embodiment 3 (by taking Jinghe sand honey raisin tree as an example)
The preparation of culture medium:
A, using distilled water as solvent, pH value 5.6,100g/L sucrose, 32mg/L boric acid, 36.5mg/L Ca (NO3)2, light
According to 35min after culture；
Pollen collection:
B, selection growth and development is good, healthy and strong Jinghe sand honey raisin tree plant of no disease and pests harm, in full-bloom stage morning time 8:00-
9:00 acquires entire spray, takes back, inserts in bottle, naturally open to flower, and pollen sheds, and carefully takes flower with tweezers, receives
Collect spare；
In-vitro pollen germination and pollen tube growth:
C, the configured culture medium of step a is instilled in the groove of concave glass slide, dips the flower that step b is obtained with writing brush
Powder is uniformly trickled down on liquid medium within, is placed in bottom and is lined in the culture dish of filter paper, covers lid and be placed on constant incubator
Interior, culture, in vitro culture time 35min are sprouted in 25 DEG C of temperature in vitro illumination；
The measurement of pollen activity:
D, by after step c in vitro culture 35min, pollen germination is thought more than pollen grain diameter to pollen tube length, i.e.,
It can start to count pollen germination rate, with the micro- sem observation pollen germination situation of OLYMPUS BX51 type, each processing is repeated 3 times,
Each to repeat 3 visuals field of random observation, each visual field pollen frequence is greater than 50, and statistics pollen germination number accounts for the ratio of pollen frequence
Example calculates pollen activity；And the pollen activity of Jinghe sand honey raisin tree difference loose powder time is measured, investigate loose powder time and pollen activity
Relationship be shown in Table 1.
Claims (1)
1. a kind of method using husky honey raisin tree in-vitro pollen germination Liquid Culture based assays sand honey raisin tree pollen activity, it is characterised in that
It follows these steps to carry out:
The preparation of culture medium:
A, using distilled water as solvent, pH value 5.6,100g/L sucrose, 32mg/L boric acid, 36.5mg/L Ca (NO3)2, illumination training
20-35min after supporting；
Pollen collection:
B, selection growth and development is good, the husky honey raisin tree plant of stalwartness of no disease and pests harm, acquires in full-bloom stage morning time 8:00-9:00
Entire spray, takes back, and inserts in bottle, naturally open to flower, and pollen sheds, and carefully takes flower with tweezers, collects spare；
In-vitro pollen germination and pollen tube growth:
C, the configured culture medium of step a is instilled in the groove of concave glass slide, it is equal dips the pollen that step b is obtained with writing brush
It on even unrestrained liquid medium within, is placed in bottom and is lined in the culture dish of filter paper, cover lid and be placed in constant incubator, temperature
Culture, in vitro culture time 25-35min are sprouted in the in vitro illumination of 20-30 DEG C of degree；
The measurement of pollen activity:
D, be more than pollen grain diameter to pollen tube length it is pollen germination by after step c in vitro culture 25-35min, starts to count
Pollen germination rate, with the micro- sem observation pollen germination situation of OLYMPUS BX51 type, each processing is repeated 3 times, each repetition with
Machine observes 3 visuals field, and each visual field pollen frequence is greater than 50, and statistics pollen germination number accounts for the ratio of pollen frequence, calculates flower
Powder vigor.
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|---|---|---|---|---|
| CN101633902A (en) * | 2009-08-25 | 2010-01-27 | 商洛学院 | In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof |
| CN101717747A (en) * | 2009-12-04 | 2010-06-02 | 西北农林科技大学 | Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen |
| US20130109748A1 (en) * | 2011-08-16 | 2013-05-02 | King Abdul Aziz City For Science And Technology | Method for obtaining extract of calligonum species and extract thereof |
| CN103571789A (en) * | 2013-11-13 | 2014-02-12 | 江苏省农业科学院 | Jasmine pollen in-vitro germination liquid culture medium and method for measuring activity of jasmine pollen |
| CN103834725A (en) * | 2014-01-09 | 2014-06-04 | 中国科学院新疆生态与地理研究所 | Analytic method of calligonum mongolicum karyotypes |
-
2019
- 2019-07-02 CN CN201910588465.6A patent/CN110218761A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101633902A (en) * | 2009-08-25 | 2010-01-27 | 商洛学院 | In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof |
| CN101717747A (en) * | 2009-12-04 | 2010-06-02 | 西北农林科技大学 | Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen |
| US20130109748A1 (en) * | 2011-08-16 | 2013-05-02 | King Abdul Aziz City For Science And Technology | Method for obtaining extract of calligonum species and extract thereof |
| CN103571789A (en) * | 2013-11-13 | 2014-02-12 | 江苏省农业科学院 | Jasmine pollen in-vitro germination liquid culture medium and method for measuring activity of jasmine pollen |
| CN103834725A (en) * | 2014-01-09 | 2014-06-04 | 中国科学院新疆生态与地理研究所 | Analytic method of calligonum mongolicum karyotypes |
Non-Patent Citations (1)
| Title |
|---|
| 康晓珊等: ""迁地保护条件下４种沙拐枣（Calligonum）的花部特征和传粉特性"", 《中国沙漠》 * |
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Application publication date: 20190910 |