CN110218629B - Kit for genetic tumor gene detection - Google Patents
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- CN110218629B CN110218629B CN201910563869.XA CN201910563869A CN110218629B CN 110218629 B CN110218629 B CN 110218629B CN 201910563869 A CN201910563869 A CN 201910563869A CN 110218629 B CN110218629 B CN 110218629B
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention discloses a kit for genetic tumor gene detection, and relates to the technical field of kits. This a kit for hereditary oncogene detects, comprises a box bod, fixedly connected with backup pad in the box body, and four sides of backup pad peg graft respectively in four sides of box body inner wall, and the below of backup pad is provided with the water storage box, and the left side of bottom in the box body of the bottom fixed connection of water storage box, peg graft at the left top of water storage box have with the oral cavity intercommunication of self oral cavity oral siphon, and the oral siphon keeps away from the one end of water storage box and runs through the left side of box body. This a kit for hereditary oncogene detects uses themogenesis mechanism through pressing reset button, arranges to the annular sponge in the cavity section of thick bamboo behind the water heating in the water storage box on, the annular sponge absorbs water and swells back and solvent bottle surface in close contact with for in water temperature conducts solvent via the solvent bottle, heats the solvent, makes because the lower precipitate that separates out of solvent temperature melts gradually.
Description
Technical Field
The invention relates to the technical field of kits, in particular to a kit for genetic tumor gene detection.
Background
In families with a family history of cancer, the treatment time is delayed and the cancer mortality rate is high, mostly because inherited tumor genes are not detected effectively in the early stage. Aiming at the hereditary tumor, the genetic tumor gene detection technology is basically adopted at home and abroad to effectively detect and timely restrain the latent state of the disease, saliva samples of the examinees are collected and DNA is extracted, and the high-throughput gene sequencer is utilized to quickly and clearly judge whether the examinees carry the hereditary tumor gene. The kit adopted in the step is a DNA extraction kit, and the kit basically comprises five reagent bottles: preservative fluid, cell lysate, impurity precipitation liquid, DNA solution and 5ml collection tube, in order to guarantee the sample activity, usually take the refrigerated mode to store under the condition that can not carry out the detection immediately after gathering, the solution in the reagent box will appear the condition that partial precipitation is appeared when the temperature is lower, need carry out the water bath when using the solution and heat up, melt the precipitate, need cool solution to room temperature after melting simultaneously and can use, the whole work efficiency of detection work has been reduced to a certain extent to such a mode of use.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a kit for genetic tumor gene detection, which can immediately perform the steps of heating and cooling when a user needs to use a solution, so as to ensure that the solution does not contain precipitates and can quickly reach the usable condition.
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a kit for hereditary oncogene detects, comprises a box bod, fixedly connected with backup pad in the box body, and four sides of backup pad are pegged graft respectively in four sides of box body inner wall, the below of backup pad is provided with the water storage box, and the left side of bottom in the box body of bottom fixed connection of water storage box, peg graft at the left top of water storage box has the oral siphon with self inner chamber intercommunication, and the one end that the oral siphon kept away from the water storage box runs through the left side of box body, it has the sealing plug to peg graft in the left end of oral siphon, the left side fixed mounting at water storage box top has themogenesis mechanism, and the middle part fixedly connected with at water storage box top and the collecting tray of self inner chamber intercommunication, peg graft at the middle part at collecting tray top has the one end of air duct, and the one end that the collecting tray was kept away from to the air duct extends right along the bottom of backup pad and links firmly mutually with the processing box.
The right side of bottom in the box body is connected to the bottom fixed connection of handling the box, and the inner chamber left side fixed mounting of handling the box has a gas collection actuating mechanism, the middle part movable mounting of back wall bottom in the processing box has the driving medium, and movable mounting has thawing mechanism on the driving medium, the first half fixed mounting of thawing mechanism is on the right side at backup pad top, and the one end of the first right side fixedly connected with exhaust of thawing mechanism, the right side of exhaust runs through the right side of box body, the right side of water storage box is passed through the aqueduct and is connected and communicate with micro water pump's water inlet, and micro water pump's delivery port and water delivery mechanism are connected, water delivery mechanism's lower half is located the backup pad, and water delivery mechanism's first half extends to the top of thawing mechanism.
Preferably, the inner chamber of collecting tray is under the narrow width form, and one side that the collecting tray inner wall is close to the water storage box inner chamber is glued and is had ventilative water-blocking film, and such structural design promotes steam and gathers, avoids the water to get into the air duct through ventilative water-blocking film simultaneously.
Preferably, the heat generating mechanism comprises a reset button, a placing frame, a separating net, a storage bag and a poking needle, the reset button is transversely inserted into the left wall of the box body, the right end of the reset button is inserted into the left side of the placing frame, the top of the placing frame is fixedly connected to the left side of the bottom of the supporting plate, the bottom of the placing frame penetrates through the left side of the top of the inside of the water storage box, four sides of the separating net are respectively and fixedly connected to the bottoms of the four sides of the inner wall of the placing frame, the poking needle is fixedly connected to one end, close to the placing frame, of the button in the reset button, one end, far away from the reset button, of the poking needle penetrates through the left inner wall of the placing frame, the storage bag is in an expansion state, 500-1000 milligrams of quicklime is filled in the storage bag, the ratio of the water to the quicklime in the water storage box is 100:1, the unit of the water is milliliter, an opening and closing sheet is hinged to the front of the box body and penetrates through the front side of the placing frame.
Preferably, the gas collection driving mechanism comprises a fixed cylinder, a telescopic airbag, a connector, sealing pieces, stabilizer bars, through holes, a connecting pipe, a connecting cylinder and a gas pipe, the top of the fixed cylinder is fixedly connected to the left side of the top in the treatment box, the top of the telescopic airbag is fixedly connected to the left side of the top in the treatment box, the side surfaces of the telescopic airbag are tightly attached to the inner wall of the fixed cylinder, the connector is fixedly connected to the middle of the bottom of the telescopic airbag and communicated with the inner cavity of the telescopic airbag, the number of the sealing pieces is four, the side surfaces of the four sealing pieces are in contact with each other and seal the inner cavity of the connector, the stabilizer bars are respectively welded on the left side and the right side of the connector, the through holes are arranged at the bottom of the fixed cylinder and communicated with the inner cavity of the fixed cylinder, the connecting pipe is longitudinally and fixedly connected to the left side and the right side of the connecting cylinder, the front side and the rear side of the connecting cylinder are respectively and fixedly connected to the bottoms of the front side and rear side of the inner wall of the through holes, the bottom of two stabilizer bars runs through two connecting cylinders respectively and runs through the bottom of connecting cylinder, the inner wall of connector agrees with mutually with the outer wall diameter of connecting pipe, and the axis of connector coincides with the axis of connecting pipe, the flange piece suit is passed through in the bottom of connecting pipe to the one end of gas-supply pipe, and the inner chamber of gas-supply pipe and the inner chamber intercommunication of connecting pipe, guaranteed that the connector can communicate with connecting pipe looks adaptation after moving down, the condition of having avoided gas leakage appears, flexible gasbag just can drive the connector and be connected with the connecting pipe after gathering up enough steam simultaneously, effort when guaranteeing gas outgoing can drive semicircle function.
Preferably, the driving medium includes carousel and oblique piece, and the carousel is through on the interior back wall of wearing bolt axle swing joint at the centre of a circle department on processing box, and the carousel is located the right side of gas-supply pipe right-hand member, and the oblique piece is the slope form and the equidistance encircles fixed connection in the front of carousel, is located the right-hand member that the positive left oblique piece of oblique piece pressed close to the gas-supply pipe for the gas-supply pipe must exert horizontal transverse force to the oblique piece when exhausting, makes its atress and drives the carousel rotation through the design of oblique piece slope form.
Preferably, the thawing mechanism comprises a hollow cylinder, a limiting ring, an annular sponge, transmission rods, semicircular pieces, inclined rods, connecting rods and a guide sliding strip, the hollow cylinder is fixedly connected to the rightmost side of the top of the supporting plate, the outer ring of the limiting ring is fixedly connected to the inner wall of the hollow cylinder, the bottom of the annular sponge is fixedly connected to the top of the limiting ring, the number of the transmission rods is two, the two transmission rods are longitudinally and respectively inserted into the left side and the right side of the top of the annular sponge, the bottom ends of the two transmission rods penetrate through the bottom of the supporting plate, through holes matched with the transmission rods are formed in the limiting ring, the semicircular pieces are symmetrically in threaded connection with the top ends of the two transmission rods, the top ends of the two transmission rods are oppositely bent, the bottom ends of the two transmission rods are hinged with the two symmetrical inclined rods, and one ends of the two inclined rods, which are opposite to each other, of the transmission rods are hinged to the top ends of the connecting rods through pin shafts, lead the draw runner setting on the rear side of connecting rod and fixed connection on the interior back wall of processing box, and lead the front of draw runner and seted up the guide chute, the round pin axle rear end sliding connection that down tube and connecting rod articulated use is in the guide chute, and the bottom of connecting rod articulates at the positive top of carousel, the connecting rod, down tube and transfer line cooperate the work of unfreezing in heat transfer to annular sponge go on, drive semi-circular spare through the transfer line simultaneously and operate and carry out the extrusion repeatedly to annular sponge, such working method cooperation annular sponge further reaches the effect of unfreezing.
Preferably, the water delivery mechanism comprises a header pipe, a T-shaped pipe, a bent pipe, an annular pipe and a water leakage port, the bottom end of the header pipe is connected and communicated with a water outlet of the micro-water pump, the top end of the header pipe is inserted into the bottom of the support plate, the T-shaped pipe is transversely embedded in the support plate, the left end of the T-shaped pipe is welded with the top of the right side of the header pipe, the bottom end of the bent pipe is welded at the top of a longer section of the T-shaped pipe, the top end of the bent pipe penetrates through the top of the support plate and extends to the upper side of the hollow cylinder, the left side of the annular pipe is welded with a shorter section of the bent pipe, the water leakage port is arranged at the bottom of the annular pipe in an equidistant surrounding manner and is communicated with the inner cavity of the annular pipe, the inner cavities of the header pipe, the T-shaped pipe, the bent pipe and the annular pipe are communicated with each other, each solvent bottle can obtain a normal thawing effect through a shunting design, and the annular sponge is ensured to be uniformly absorbed and expanded orderly through the surrounding design of the water leakage port, the solvent bottle was subjected to thawing treatment.
Preferably, the drain fitting includes drain pipe and inflation bag, and the drain pipe transversely alternates in the right wall of box body, and the left end of drain pipe run through the bottom on a cavity section of thick bamboo right side and with the inner chamber intercommunication of a cavity section of thick bamboo, the inflation bag splices at the inner wall of drain pipe, the inflation bag is made for expend with heat and contract with cold material, the internal and external air of box under the non-user state, self-sealing guarantees the normal implementation of function of unfreezing during the use.
The invention provides a kit for genetic tumor gene detection. The method has the following beneficial effects:
(1) this a kit for hereditary tumour gene detects, heat the water body through chemical reaction, the temperature conducts to the interior liquid of bottle via the solvent bottle, accelerate gene detection work efficiency, when reaching the effect of unfreezing, the hot steam that the reaction generated is filled up and is played the supplementary effect of unfreezing to other reagents in the box body, when unfreezing to the solvent bottle that produces the deposit easily, the effect of unfreezing of hot steam is slow relatively, prevented that other reagents that need not use from unfreezing the condition appearance that influences its quality sooner in non-use phase.
(2) This a kit for hereditary oncogene detects, when heating the water body and unfreezing to the solvent bottle, the hot steam synchronous drive thawing mechanism that the water body discharged extrudes the sponge, will be located the sponge middle part and crowd to the in-process of solvent bottle in one side of keeping away from the solvent bottle, further extrude moisture and unfreeze with the contact of solvent bottle, the water of extruding simultaneously is collected and is soaked the thawing to the position that extremely easily produces the sediment in the solvent bottle in the hollow cylinder, thawing mechanism is in the operation in-process equally with the temperature through the leading-in annular sponge of mode of contact, play certain supplementary effect to its temperature, further ensure the effect of unfreezing of solvent.
(3) This a kit for hereditary tumour gene detects can keep the inside and outside circulation of air of reagent box through drainage piece in the cold-stored stage of kit, ensures that air conditioning gets into in the reagent box fast and cools down reagent and solvent, seals drainage piece through hot water when the kit unfreezes the use, ensures that the bottom that the solvent bottle produced the deposit easily obtains the effect of unfreezing that hot water soaked all the time, drainage piece can carry out the working procedure of solvent cooling to room temperature at the hot water of discharging in the twinkling of an eye that the heating effect ended simultaneously and steam.
(4) The kit for genetic tumor gene detection can effectively divide absorbed water into two parts for storage by using the annular sponge as a heat conducting medium, one part is one side close to the solvent bottle and contacts with the surface of the solvent bottle at a low temperature first, wherein the heat of the water is conducted into the solvent bottle first, the heating mode avoids the situation that the whole heat is absorbed by the surface of the solvent bottle once, the surface of the solvent bottle is unfrozen, but the internal precipitate does not accept sufficient heat for unfreezing, and the situation that the solvent bottle is cracked due to the direct contact of the water at a higher temperature and the surface of the solvent bottle can be avoided, the repeated extrusion of the semicircular piece is matched in the process of repeated water absorption and storage, so that hot water in the annular sponge is in a continuous state and continuously contacts with the solvent bottle, and meanwhile, cold water is synchronously discharged into the bottom of the hollow cylinder, the surface of the solvent bottle is guaranteed to be continuously heated, the final thawing work is finished and the temperature is reduced when all water bodies are discharged into the hollow cylinder, and the solvent bottle is assisted to enter a cooling stage.
Drawings
FIG. 1 is a cross-sectional view of the structure of the present invention;
FIG. 2 is a perspective view of the structure of the present invention;
FIG. 3 is an enlarged view of the structure at A in FIG. 1 according to the present invention;
FIG. 4 is an enlarged view of the structure at B in FIG. 1 according to the present invention;
FIG. 5 is a perspective view of the water delivery mechanism of the present invention viewed from the bottom;
FIG. 6 is a perspective view of a transmission member of the present invention;
fig. 7 is a side view of the drain member of the present invention.
In the figure: 1 box body, 2 supporting plates, 3 water storage box, 4 water inlet pipe, 5 sealing plug, 6 heat generating mechanism, 7 collecting tray, 8 air and water blocking film, 9 air guide pipe, 10 processing box, 11 driving mechanism, 12 driving part, 13 unfreezing mechanism, 14 water discharging part, 15 micro water pump, 16 water conveying mechanism, 17 opening and closing sheet, 601 reset button, 602 placing frame, 603 separating net, 604 storage bag, 605 poking needle, 111 fixing cylinder, 112 telescopic air bag, 113 connecting head, 114 sealing sheet, 115, 116 through hole, 117 connecting pipe, 118 connecting cylinder, 119 air conveying pipe, 121 rotating disc, 122 inclined sheet, 131 hollow cylinder, 132 limiting ring, 133 annular sponge, 134 driving rod, 135 semicircular part, 136 inclined rod, 137 connecting rod, 138 guide sliding strip, 161 main pipe, 162T-shaped pipe, 163 bent pipe, 164 annular pipe, 165 water leaking port, 141 water discharging pipe and 142 expansion bag.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
Referring to fig. 1-7, the present invention provides a technical solution: a kit for genetic tumor gene detection comprises a box body 1, a support plate 2 is fixedly connected in the box body 1, four sides of the support plate 2 are respectively inserted in four sides of the inner wall of the box body 1, the top of the box body 1 is provided with placing openings in a five-row eight-column shape, the bottom end of each placing opening penetrates through the inner top of the box body 1, the top of the support plate 2 is provided with five-row eight-column placing openings corresponding to the placing openings on the box body 1, the placing openings on the rightmost side are large in diameter and used for placing solution bottles, a water storage box 3 is arranged below the support plate 2, the bottom of the water storage box 3 is fixedly connected to the left side of the inner bottom of the box body 1, the top of the left side of the water storage box 3 is inserted with a water inlet pipe 4 communicated with an inner cavity of the water inlet pipe 4, one end of the water inlet pipe 4, far away from the water storage box 3, penetrates through the left side of the box body 1, a sealing plug 5 is inserted in the left end of the water inlet pipe 4, a heat generating mechanism 6 is fixedly installed on the left side of the top of the water storage box 3, and the middle part fixedly connected with at 3 tops of water storage box and the collecting tray 7 of self inner chamber intercommunication, the middle part at 7 tops of collecting tray is pegged graft and is had the one end of air duct 9, and the one end that collecting tray 7 was kept away from to air duct 9 extends along the bottom of backup pad 2 right and links firmly with processing box 10 mutually.
The bottom of the processing box 10 is fixedly connected with the right side of the bottom in the box body 1, the left side of the inner cavity of the processing box 10 is fixedly provided with a gas collection driving mechanism 11, the middle part of the bottom of the rear wall in the processing box 10 is movably provided with a transmission part 12, and the driving member 12 is movably provided with a thawing mechanism 13, the upper half part of the thawing mechanism 13 is fixedly arranged at the right side of the top of the supporting plate 2, and the right side of the upper half part of the unfreezing mechanism 13 is fixedly connected with one end of a drainage piece 14, the other end of the drainage piece 14 penetrates through the right side of the box body 1, the right side of the water storage box 3 is connected and communicated with a water inlet of a micro water pump 15 through a water guide pipe, and the water outlet of the micro water pump 15 is connected with the water delivery mechanism 16, the lower half part of the water delivery mechanism 16 is positioned in the supporting plate 2, and the upper half of the water delivery mechanism 16 extends to the upper part of the unfreezing mechanism 13, the inner cavity of the collecting tray 7 is narrow at the upper part and wide at the lower part, and a ventilation water-blocking film 8 is glued on one side of the inner wall of the collecting tray 7 close to the inner cavity of the water storage box 3.
The heat generating mechanism 6 comprises a reset button 601, a placing frame 602, a separating net 603, a storage bag 604 and a poking needle 605, wherein the reset button 601 is transversely inserted and connected in the left wall of the box body 1, the right end of the reset button 601 is inserted and connected in the left side of the placing frame 602, the top of the placing frame 602 is fixedly connected to the left side of the bottom of the supporting plate 2, the bottom of the placing frame 602 penetrates through the left side of the inner top of the water storage box 3, four sides of the separating net 603 are respectively and fixedly connected to the bottoms of four sides of the inner wall of the placing frame 602, one end, close to the placing frame 602, of the button in the reset button 601 is fixedly connected with the poking needle 605, one end, far away from the reset button 601, of the poking needle 605 penetrates through the inner left wall of the placing frame 602, the front surface of the box body 1 is hinged with an opening and closing sheet 17, the front side of the placing frame 602 is penetrated by the opening and closing sheet, the storage bag 604 is in an expansion state, quicklime of 500-1000 milligrams is filled in the storage bag 604, the ratio of the water amount in the quicklime in the water storage box 3 to the quicklime is 100:1, the unit of water yield is the milliliter, and this proportion is the reasonable proportion that reachs through many times of experiments, ensures that reaction temperature can not too high and leads to the condition of kit damage to appear, and the front of box body 1 articulates there is the piece 17 that opens and shuts, and opens and shut the piece 17 and run through the front side of placing frame 602, changes disposable storage bag 604 through opening the piece 17 that opens and shuts.
The gas collecting driving mechanism 11 comprises a fixed cylinder 111, a telescopic air bag 112, a connecting head 113, sealing pieces 114, stabilizer bars 115, a port 116, a connecting pipe 117, a connecting cylinder 118 and a gas conveying pipe 119, wherein the top of the fixed cylinder 111 is fixedly connected to the left side of the top in the processing box 10, the top of the telescopic air bag 112 is fixedly connected to the left side of the top in the processing box 10, the side surfaces of the telescopic air bag 112 are tightly attached to the inner wall of the fixed cylinder 111, the connecting head 113 is fixedly connected to the middle of the bottom of the telescopic air bag 112 and communicated with the inner cavity of the telescopic air bag 112, the number of the sealing pieces 114 is four, the side surfaces of the four sealing pieces 114 are in contact with each other and seal the inner cavity of the connecting head 113, the stabilizer bars 115 are respectively welded to the left side and the right side of the connecting head 113, the port 116 is arranged at the bottom of the fixed cylinder 111 and communicated with the inner cavity thereof, the connecting pipe 117 is longitudinally arranged in the port 116, the connecting cylinder 118 is longitudinally and fixedly connected to the left side and the right side of the connecting pipe 117 respectively, the front side and the back side of a connecting cylinder 118 respectively with the bottom fixed connection of both sides around the opening 116 inner wall, the bottom of two stabilizer bars 115 runs through two connecting cylinders 118 respectively and runs through the bottom of connecting cylinder 118, the inner wall of connector 113 agrees with the outer wall diameter of connecting pipe 117 mutually, and the axis of connector 113 coincides with the axis of connecting pipe 117, such structural design has guaranteed that connector 113 can be connected with connecting pipe 117 pair after moving down, can effectively guarantee that the removal route of connector 113 is fixed through stabilizer bar 115 and connecting cylinder 118's cooperation simultaneously, flange piece suit is passed through to the one end of gas-supply pipe 119 in the bottom of connecting pipe 117, and the inner chamber of gas-supply pipe 119 and the inner chamber intercommunication of connecting pipe 117.
The transmission part 12 comprises a rotary table 121 and an inclined sheet 122, the rotary table 121 is movably connected to the inner rear wall of the processing box 10 through a bolt penetrating shaft at the center of a circle, the rotary table 121 is located on the right side of the right end of the air conveying pipe 119, the inclined sheet 122 is inclined and fixedly connected to the front of the rotary table 121 in an equidistant mode, the inclined sheet 122 located on the left side of the front of the inclined sheet 122 is close to the right end of the air conveying pipe 119, and the inclined sheet 122 in the inclined state ensures that the rotary table 121 rotates clockwise when blown by air flow.
The thawing mechanism 13 comprises a hollow cylinder 131, a limiting ring 132, an annular sponge 133, transmission rods 134, a semicircular part 135, inclined rods 136, a connecting rod 137 and a guide sliding strip 138, wherein the hollow cylinder 131 is fixedly connected to the rightmost side of the top of the support plate 2, the outer ring of the limiting ring 132 is fixedly connected to the inner wall of the hollow cylinder 131, the bottom of the annular sponge 133 is fixedly connected to the top of the limiting ring 132, the number of the transmission rods 134 is two, the two transmission rods 134 are longitudinally and respectively inserted into the left side and the right side of the top of the annular sponge 133, the bottom ends of the two transmission rods 134 both penetrate through the bottom of the support plate 2, through holes matched with the transmission rods 134 are formed in the limiting ring 132, the semicircular part 135 is symmetrically and threadedly connected to the top ends of the two transmission rods 134, the top ends of the two transmission rods 134 are bent relatively, the bottom ends of the two transmission rods 134 are hinged with the two symmetrical inclined rods 136, and one ends of the two inclined rods 136, which are opposite to each other, of the transmission rods 134 are hinged to the top ends of the connecting rod 137 through a pin shaft, the slide guiding strip 138 is arranged at the rear side of the connecting rod 137 and fixedly connected to the inner rear wall of the processing box 10, the front of the slide guiding strip 138 is provided with a slide guiding groove, the rear end of a pin shaft used by hinging the inclined rod 136 with the connecting rod 137 is slidably connected in the slide guiding groove, the bottom end of the connecting rod 137 is hinged to the top of the front of the turntable 121, a solution bottle is placed in the box body 1 and then positioned in the hollow cylinder 131, the annular sponge 133 is provided with a slide groove matched with the transmission rod 134, the bottom of the right wall in the processing box 10 is transversely inserted with an exhaust pipe, and the right end of the exhaust pipe penetrates through the right side of the box body 1 and is communicated with the outside, so that hot air in the processing box 10 can be exhausted, the damage of the processing box 10 caused by overlarge pressure is avoided, based on the physical rule that hot air flows upwards, when water in the water storage box 3 reacts with quicklime, hot steam exhausted from the liquid meter is collected by the collecting disc 7 and exhausted into the telescopic air bag 112 through the air duct 9, the collection disc 7 only collects gas molecules but does not suck water molecules through the special design of the air-permeable water-blocking film 8 in the collection disc 7, so that hot water is enabled to completely enter the unfreezing mechanism 13 to unfreeze a solvent which is easy to precipitate, meanwhile, the telescopic air bag 112 expands downwards and drives the connecting head 113 to be connected with the connecting pipe 117 under the state of continuously charging hot air, the hot air is discharged through the air pipe 119 when being released, thrust is generated on the inclined sheet 122 connected to the surface of the rotary disc 121 so as to drive the rotary disc 121 to rotate, the rotary disc 121 drives the top end of the connecting rod 137 to reciprocate up and down along the sliding guide strip 138 after rotating, the driving rod 134 is further pulled by the inclined rod 136 to reciprocate up and down, so that the semicircular piece 135 reciprocates up and down to extrude the annular sponge 133 after water absorption, the annular sponge 133 further contacts with the surface of a solvent bottle in the extrusion process and causes the aperture of one side of the annular sponge 133 contacting with the solvent bottle to be enlarged based on the deformation effect, the water accumulated in the ring-shaped sponge 133 is discharged, the thawing rate of the solvent is further accelerated, and meanwhile, the extruded water flows downwards along the surface of the solvent bottle and accumulates at the bottom of the inner cavity of the hollow cylinder 131, so that the bottom of the solvent bottle generating the sediment is always soaked in hot water, and the effect of removing the sediment in a targeted manner is achieved.
The water delivery mechanism 16 comprises a main pipe 161, a T-shaped pipe 162, a bent pipe 163, an annular pipe 164 and a water leakage port 165, the bottom end of the main pipe 161 is connected and communicated with the water outlet of the micro-water pump 15, the top end of the main pipe 161 is inserted into the bottom of the support plate 2, the T-shaped pipe 162 is transversely embedded in the support plate 2, the left end of the T-shaped pipe 162 is welded with the top of the right side of the main pipe 161, the bottom end of the bent pipe 163 is welded on the top of the longer section of the T-shaped pipe 162, the top end of the bent pipe 163 penetrates through the top of the support plate 2 and extends to the upper side of the hollow cylinder 131, the left side of the annular pipe 164 is welded with the shorter section of the bent pipe 163, the water leakage port 165 is arranged at the bottom of the annular pipe 164 in an equidistant surrounding manner and is communicated with the inner cavity of the annular pipe 164, the inner cavities of the main pipe 161, the T-shaped pipe 162, the bent pipe 163 and the annular pipe 164 are communicated with each other, five groups of the bent pipe 163 and the annular pipe 164 are welded at the top of the T-shaped pipe 162 in an equidistant manner, and the solution bottle is located in the annular pipe 164, the water leakage opening 165 is located right above the annular sponge 133, when a user needs to use a solvent, the user uses the heat generation mechanism 6 by pressing the reset button 601, the water in the water storage box 3 is heated and then pumped into the water delivery mechanism 16 through the operation of the micro water pump 15 and is discharged to the annular sponge 133 in the hollow cylinder 131, the annular sponge 133 is closely contacted with the surface of the solvent bottle after absorbing water and expanding, so that the temperature of the water is transferred into the solvent through the solvent bottle, the solvent is heated, precipitates separated out due to the low temperature of the solvent are gradually melted, meanwhile, hot steam released in the process that the water in the water storage box 3 continuously reacts with quicklime is also discharged through the water delivery mechanism 16, and the hot steam spreading between the inner top of the box body 1 and the support plate 2 can assist other reagents to thaw while effectively assisting the temperature rise of the solvent bottle to melt the precipitates, the kit after refrigerated transport can be quickly thawed to reach the usable condition of the reagent when in use, and the working efficiency of gene detection work is accelerated.
The drainage member 14 comprises a drainage pipe 141 and an expansion bag 142, the drainage pipe 141 is transversely inserted into the right wall of the box body 1, the left end of the drainage pipe 141 penetrates through the bottom of the right side of the hollow cylinder 131 and is communicated with the inner cavity of the hollow cylinder 131, the expansion bag 142 is glued to the inner wall of the drainage pipe 141, when hot water starts to be accumulated in the hollow cylinder 131, the hot water flows into the drainage pipe 141 based on the position design of the drainage pipe 141, the expansion bag 142 immediately expands after contacting with the hot water and seals the inner cavity of the drainage pipe 141, the hot water is ensured to be accumulated in the hollow cylinder 131 in the subsequent process of continuously filling the hot water, when the water in the water storage box 3 is reacted with quick lime, the temperature of the water starts to gradually decrease, and the expansion bag 142 recovers the original shape after being heated and enables the water to be discharged out of the box body 1 through the drainage pipe 141.
When the device is used, the reset button 601 is pressed to drive the poking needle 605 to move rightwards to the placing frame 602 to puncture the storage bag 604, the quicklime falls into the water storage box 3 through the separation net 603 to react with water, hot water is pumped into the water guide pipe through the micro water pump 15 and is discharged into the annular sponge 133 through the header pipe 161, the T-shaped pipe 162, the bent pipe 163, the annular pipe 164 and the water leakage port 165, the annular sponge 133 absorbs water to expand and contacts with the surface of the solution bottle to enable the temperature of the solution bottle to rise and melt precipitates, hot steam produced in the reaction process of the water and the quicklime is discharged into the telescopic air bag 112 through the collecting disc 7 and the air duct 9, the telescopic air bag 112 expands downwards along the inner cavity of the fixed cylinder 111 and drives the connecting head 113 to move downwards, the connecting head 113 moves downwards along the connecting cylinder 118 through the stabilizing rod 115, the connecting head 113 is sleeved on the connecting pipe 117, the connecting pipe 117 extrudes the sealing sheet 114 to enable hot air to circulate and be sprayed to the inclined sheet 122 through the air duct 119, the rotating disc 121 rotates by taking a pin shaft as an axis and drives the bottom end of the connecting rod 137 to do circular motion, the pin shaft used by hinging the connecting rod 137 and the inclined rod 136 makes reciprocating up-and-down motion along the slide guiding strip 138, the inclined rod 136 synchronously moves and drives the transmission rod 134 to synchronously move, the semicircular piece 135 synchronously moves to extrude the annular sponge 133, water in the annular sponge 133 is extruded and further contacts with the surface of the solution bottle to heat the solution bottle, the extruded water is accumulated at the bottom of the inner cavity of the hollow cylinder 131, hot water bath is carried out on the bottom of the solution bottle which is easy to generate deposit accumulation, when hot water begins to accumulate, part of hot water flows into the drain pipe 141 and contacts with the expansion bag 142 to be heated and expanded to seal the drain pipe 141, when heating and cooling are completed, the expansion bag 142 shrinks and discharges the water out of the box body 1, and the disposable storage bag 604 is replaced by opening the opening and closing piece 17.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. A kit for genetic tumor gene detection, comprising a kit body (1), characterized in that: the box body (1) is internally and fixedly connected with a supporting plate (2), four sides of the supporting plate (2) are respectively inserted in four sides of the inner wall of the box body (1), a water storage box (3) with a heat preservation effect is arranged below the supporting plate (2), the bottom of the water storage box (3) is fixedly connected with the left side of the bottom in the box body (1), the top of the left side of the water storage box (3) is inserted with a water inlet pipe (4) communicated with an inner cavity of the water inlet pipe, one end of the water inlet pipe (4) far away from the water storage box (3) penetrates through the left side of the box body (1), a sealing plug (5) is inserted in the left end of the water inlet pipe (4), the left side of the top of the water storage box (3) is fixedly provided with a heat generating mechanism (6), the middle part of the top of the water storage box (3) is fixedly connected with a collecting tray (7) communicated with the inner cavity of the water storage box, and the middle part of the top of the collecting tray (7) is inserted with one end of an air duct (9), one end of the air duct (9) far away from the collecting tray (7) extends rightwards along the bottom of the supporting plate (2) and is fixedly connected with the processing box (10);
the bottom of the processing box (10) is fixedly connected with the right side of the bottom in the box body (1), the left side of the inner cavity of the processing box (10) is fixedly provided with a gas collecting driving mechanism (11), the middle part of the bottom of the inner back wall of the processing box (10) is movably provided with a transmission part (12), and the driving piece (12) is movably provided with a thawing mechanism (13), the upper half part of the thawing mechanism (13) is fixedly arranged on the right side of the top of the supporting plate (2), and the right side of the upper half part of the unfreezing mechanism (13) is fixedly connected with one end of a drainage piece (14), the other end of the drainage piece (14) penetrates through the right side of the box body (1), the right side of the water storage box (3) is connected and communicated with a water inlet of a micro water pump (15) through a water guide pipe, the water outlet of the micro water pump (15) is connected with the water delivery mechanism (16), the lower half part of the water delivery mechanism (16) is positioned in the support plate (2), the upper half part of the water conveying mechanism (16) extends to the upper part of the unfreezing mechanism (13);
the gas collecting driving mechanism (11) comprises a fixed cylinder (111), a telescopic air bag (112) used for collecting hot steam, a connector (113) used for transmitting hot steam, sealing sheets (114), stabilizer bars (115), a port (116), a connecting pipe (117), a connecting cylinder (118) and an air conveying pipe (119), the top of the fixed cylinder (111) is fixedly connected to the left side of the top in the processing box (10), the top of the telescopic air bag (112) is fixedly connected to the left side of the top in the processing box (10), the side surfaces of the telescopic air bag (112) are tightly attached to the inner wall of the fixed cylinder (111), the connector (113) is fixedly connected to the middle of the bottom of the telescopic air bag (112) and communicated with the inner cavity of the telescopic air bag (112), the number of the sealing sheets (114) is four, the side surfaces of the four sealing sheets (114) are in contact with each other and close the inner cavity of the connector (113), the stabilizer bars (115) are respectively welded to the left side and the right side of the connector (113), the port (116) is arranged at the bottom of the fixed barrel (111) and communicated with the inner cavity of the fixed barrel, the connecting pipe (117) is longitudinally arranged in the port (116), the connecting barrels (118) are longitudinally and respectively fixedly connected to the left side and the right side of the connecting pipe (117), the front side and the rear side of the connecting barrels (118) are respectively and fixedly connected with the bottoms of the front side and the rear side of the inner wall of the port (116), the bottom ends of the two stabilizer bars (115) respectively penetrate through the two connecting barrels (118) and the bottom ends of the connecting barrels (118), the inner wall of the connector (113) is matched with the outer wall diameter of the connecting pipe (117), the central axis of the connector (113) is coincided with the central axis of the connecting pipe (117), one end of the air pipe (119) is sleeved at the bottom end of the connecting pipe (117) through a flange sheet, and the inner cavity of the air pipe (119) is communicated with the inner cavity of the connecting pipe (117);
the transmission part (12) comprises a rotary disc (121) used for driving the unfreezing mechanism (13) and an inclined sheet (122) used for bearing air flow, the rotary disc (121) is movably connected to the inner rear wall of the processing box (10) through a bolt shaft penetrating through the circle center, the rotary disc (121) is positioned on the right side of the right end of the air conveying pipe (119), the inclined sheet (122) is inclined and fixedly connected to the front of the rotary disc (121) in an equidistant surrounding mode, and the inclined sheet (122) positioned on the left side of the front of the inclined sheet (122) is close to the right end of the air conveying pipe (119);
the unfreezing mechanism (13) comprises a hollow cylinder (131) for treating the solvent bottle, a limiting ring (132), an annular sponge (133) for heating the solvent bottle, transmission rods (134), a semi-circular piece (135) for promoting heating, an inclined rod (136), a connecting rod (137) and a guide sliding strip (138), wherein the hollow cylinder (131) is fixedly connected to the rightmost side of the top of the supporting plate (2), the outer ring of the limiting ring (132) is fixedly connected to the inner wall of the hollow cylinder (131), the bottom of the annular sponge (133) is fixedly connected to the top of the limiting ring (132), the number of the transmission rods (134) is two, the two transmission rods (134) are longitudinally and respectively inserted into the left side and the right side of the top of the annular sponge (133), the bottom ends of the two transmission rods (134) penetrate through the bottom of the supporting plate (2), through holes matched with the transmission rods (134) are formed in the limiting ring (132), semicircle spare (135) are the top of symmetry form threaded connection at two transfer lines (134), and the top of two transfer lines (134) is buckled relatively, the bottom of two transfer lines (134) articulates there are two down tubes (136) that are the symmetry form, and the one end of two down tubes (136) back of the body transfer line (134) all articulates the top at connecting rod (137) through the round pin axle, lead draw runner (138) and set up at the rear side of connecting rod (137) and fixed connection on the interior back wall of handling box (10), and lead the front of draw runner (138) and seted up the guide chute, round pin axle rear end sliding connection that draw runner (136) and connecting rod (137) articulated use is in the guide chute, and the bottom of connecting rod (137) articulates the top in carousel (121) front.
2. The kit for genetic oncogene detection according to claim 1, wherein: the inner cavity of the collecting tray (7) is narrow at the top and wide at the bottom, and one side of the inner wall of the collecting tray (7) close to the inner cavity of the water storage box (3) is glued with an air-permeable water-blocking film (8).
3. The kit for genetic oncogene detection according to claim 1, wherein: the heat generating mechanism (6) comprises a reset button (601), a placing frame (602), a separating net (603), a storage bag (604) and a poking needle (605) for triggering a heating function, the reset button (601) is transversely inserted and connected in the left wall of the box body (1), the right end of the reset button (601) is inserted and connected in the left side of the placing frame (602), the top of the placing frame (602) is fixedly connected to the left side of the bottom of the supporting plate (2), the bottom of the placing frame (602) penetrates through the left side of the inner top of the water storage box (3), four sides of the separating net (603) are respectively and fixedly connected to the bottoms of the four sides of the inner wall of the placing frame (602), one end, close to the placing frame (602), of the inner button of the reset button (601) is fixedly connected with the poking needle (605), one end, far away from the reset button (601), of the poking needle (605) penetrates through the inner left wall of the placing frame (602), an opening and closing sheet (17) is hinged to the front face of the box body (1), and the opening and closing sheet (17) penetrates the front side of the placing frame (602).
4. The kit for genetic oncogene detection according to claim 1, wherein: the water delivery mechanism (16) comprises a main pipe (161), a T-shaped pipe (162), a bent pipe (163), an annular pipe (164) and a water leakage port (165) for shunting, the bottom end of the main pipe (161) is connected and communicated with a water outlet of the micro water pump (15), the top end of the main pipe (161) is inserted into the bottom of the support plate (2), the T-shaped pipe (162) is transversely embedded in the support plate (2), the left end of the T-shaped pipe (162) is welded with the top of the right side of the main pipe (161), the bottom end of the bent pipe (163) is welded with the top of the longer section of the T-shaped pipe (162), the top end of the bent pipe (163) penetrates through the top of the support plate (2) and extends to the upper part of the hollow cylinder (131), the left side of the annular pipe (164) is welded with the shorter section of the bent pipe (163), the water leakage port (165) is arranged at the bottom of the annular pipe (164) in an equidistant surrounding manner and is communicated with the inner cavity of the annular pipe (164), the inner cavities of the header pipe (161), the T-shaped pipe (162), the bent pipe (163) and the annular pipe (164) are communicated with each other.
5. The kit for genetic oncogene detection according to claim 1, wherein: the drainage piece (14) comprises a drainage pipe (141) used for communicating air inside and outside the box body (1) and an expansion bag (142) used for closing and opening the drainage pipe (141), the drainage pipe (141) is transversely inserted into the right wall of the box body (1), the left end of the drainage pipe (141) penetrates through the bottom on the right side of the hollow cylinder (131) and is communicated with the inner cavity of the hollow cylinder (131), and the expansion bag (142) is glued on the inner wall of the drainage pipe (141).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910563869.XA CN110218629B (en) | 2019-06-26 | 2019-06-26 | Kit for genetic tumor gene detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910563869.XA CN110218629B (en) | 2019-06-26 | 2019-06-26 | Kit for genetic tumor gene detection |
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| CN110218629A CN110218629A (en) | 2019-09-10 |
| CN110218629B true CN110218629B (en) | 2022-04-19 |
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| CN113549526B (en) * | 2020-12-14 | 2024-03-01 | 西部医学科技集团有限公司 | DNA reaction kit for biological breeding |
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| EP1808698A1 (en) * | 2006-01-13 | 2007-07-18 | F.Hoffmann-La Roche Ag | Reagent kit and analyzer |
| CN107054885B (en) * | 2017-03-28 | 2021-09-10 | 苏州市广济医院 | Portable refrigerator and refrigeration equipment |
| CN207129418U (en) * | 2017-06-29 | 2018-03-23 | 吉尔生物科技（天津）有限公司 | A kind of Pseudomonas fluorescens detection kit |
| CN208560130U (en) * | 2018-05-28 | 2019-03-01 | 北京普赞生物技术有限公司 | A kind of food inspection kit |
| CN208761292U (en) * | 2018-09-11 | 2019-04-19 | 江西赛基生物技术有限公司 | A kind of six link detection reagent kit of cell factor convenient for dropping liquid |
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