CN110208073A - Micro-fluidic sample concentration device and application method based on photo-thermal evaporation - Google Patents
Micro-fluidic sample concentration device and application method based on photo-thermal evaporation Download PDFInfo
- Publication number
- CN110208073A CN110208073A CN201910516415.7A CN201910516415A CN110208073A CN 110208073 A CN110208073 A CN 110208073A CN 201910516415 A CN201910516415 A CN 201910516415A CN 110208073 A CN110208073 A CN 110208073A
- Authority
- CN
- China
- Prior art keywords
- gas
- thermal
- photo
- layer
- evaporation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4022—Concentrating samples by thermal techniques; Phase changes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4022—Concentrating samples by thermal techniques; Phase changes
- G01N2001/4027—Concentrating samples by thermal techniques; Phase changes evaporation leaving a concentrated sample
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention discloses a kind of micro-fluidic sample concentration devices and application method based on photo-thermal evaporation；Micro-fluidic sample concentration device based on photo-thermal evaporation, including sample solution channel, it is characterized by: being provided with hole optical thermal evaporation layer above sample solution channel, it is provided with carrier gas passage above hole optical thermal evaporation layer, is provided with light source on the outside of hole optical thermal evaporation layer；The hole optical thermal evaporation layer is made of hydrophilic porous photo-thermal dielectric layer and hydrophobic gas diffusion layer；The hydrophilic porous photo-thermal dielectric layer generates capillary suction force to liquid, sample solution is set to enter hydrophilic porous photo-thermal dielectric layer, simultaneously, the incident light that hydrophilic porous photo-thermal dielectric layer absorbs light source, which generates photo-thermal effect, makes sample solution heat absorption evaporation generate gas, and then realizes the evaporation and concentration to sample solution；The hydrophobic gas diffusion layer is hydrophobic structure, is located at hydrophilic porous photo-thermal dielectric layer top；The present invention can be widely used in the fields such as crystal purification, pharmaceutical synthesis, food engineering.
Description
Technical field
The present invention relates to micro-fluidic field, more particularly to a kind of micro-fluidic sample concentration device based on photo-thermal evaporation and
Application method.
Background technique
Micro-fluidic chip is grown up by the 1990s in analytical chemistry field, as analysis system, biological medicine
Device, chemistry and biochemical tools are just playing increasingly important role.Micro-fluidic chip with its great specific surface area,
Extremely low sample requirements, realize high analysis efficiency, many micro-fluidic chips can within the time of several seconds to tens of seconds,
Measurement, separation or other more complicated operations are completed with the amount of samples required far below Routine Test Lab, this is other routines
What analysis, detection means were difficult to reach.In addition to this, compared with traditional analysis, detection method, micro-fluidic chip, which also has, is
External connection of uniting is few, pollution less, it can be achieved that in real time, it is in situ, continuously detect and facilitate and realize the series of advantages such as batch experiment,
Micro-fluidic chip has great application prospect in following field of scientific study.
During biochemistry detection and chemical industry synthesis, sample concentration is an important intermediate link, to analysis detection, knot
The fields such as brilliant purification, pharmaceutical synthesis, food engineering are most important, and related actual application is seen everywhere: there is researcher's expression,
When being detected using NMR (nuclear magnetic resonance technique) to the human cerebrospinal fluid of 600nL, because of extremely low sample quality and equipment
To object to be measured there are certain reaction time, in order to detect the metabolite of 1nM, researcher is needed at least by sample
The concentration of product promotes 9 times and continuously detects 18 hours --- there it can be seen that being directed to many specific research environments, seldom
Sample size, extremely low sample concentration actually cause very big influence to scientific experiment or engineering, and this and micro-fluidic core
The self performance perfection of piece suits, the important use that " sample concentration " becomes for micro-fluidic chip.Main sample is dense
Contracting mode includes liquid-phase extraction, membrane separation process, electrocapillary phoresis, evaporation and concentration etc..Wherein, evaporation and concentration has easy to operate, general
Adaptive is strong, the advantages such as low to external supplemental equipment requirement, this greatly causes the interest of researchers and research enthusiasm.
The core link of micro-fluidic chip based on evaporation and concentration is in the runner or cavity of micro-meter scale to sample
Solvent composition is evaporated, and the mode being concentrated by evaporation at present mainly includes passive type and active two major classes.Based on evaporation and concentration
Passive type micro-fluidic chip mainly evaporate sample solution naturally in microchannel, to reach to sample solution
Concentrated effect.Although the microfluid analysis method using the technology has certain superiority compared to traditional analysis, its
There are still the lower problem of additional issue rate, concentration speed, the prospect promoted and applied is not superior.And based on evaporation and concentration
Active micro-fluidic chip then mainly realizes the fast of sample solution by modes such as gas phase decompression, forced convertion, electric heating and photo-thermal
Speed concentration, has great application prospect.Wherein, the evaporation mode of gas phase decompression is to micro fluidic device air-tightness and mechanical strength
It is more demanding, it is chiefly used in the links such as surface preparation, micro Process.The evaporation mode of forced convertion is widely used in easy volatile solvent
Removal, however its application is limited by working medium physical property itself, it is difficult to it is used for the lower working medium of volatility.With MEMS (Micro-
Electromechanical Systems, MEMS) technology development, electric heating evaporation and photo-thermal evaporation become the widest
The active evaporation technique of general application.Electric heating evaporation is embedded in heating element by micro Process means in micro fluidic device, passes through electricity
Heating method is evaporated fluid, but its chip structure is complicated, and design processing cost is higher.Compared with electrothermal method, photo-thermal
Evaporation carries out non-contact thermal to fluid and drives evaporation process, without the micro- of complexity by the photo-thermal effect between light and fluid
Processing technology and external equipment assist.Since spread speed is fast in the medium for light, and sphere of action is accurately adjustable, in micro-fluidic dress
Set middle progress fluid heating have it is natural be quick on the draw, the advantage that part is controllable, to obtain the extensive concern of researcher.
In conclusion the micro-fluidic chip based on evaporation and concentration using photothermal technique has great application prospect, but
There are still some problems constrains the application on a large scale of its investment.Firstly, the material of production microchannel more common at present is
Dimethyl silicone polymer (PDMS, polydimethylsiloxane), although PDMS material has certain gas permeability, it is led
" packaged type " design of stream is still difficult to meet the requirement of system Quick air-discharge in the case of high-efficiency evaporating, and excessive gas is micro- logical
Accumulation in road can generate inhibiting effect to evaporation process, and gas liquid two-phase flow is formed in liquid phase runner, increase fluid stream
Dynamic resistance, the stability of Interference Flow.Furthermore when being used for micro-fluidic chip to sample solution concentration and evaporation, because of its microchannel
The lesser feature of intrinsic characteristic size, it is difficult to which guarantee has a reasonable gas-liquid disengagement area, this greatly reduces evaporation
Efficiency.Finally, the evaporation efficiency of the micro-fluidic chip entirety based on evaporation and concentration need to be improved, evaporation relatively common at present
Enrichment facility is that photothermal conversion medium, such as gold, silver noble metal are loaded in microchannel, is shone by controlling light beam it
It penetrates to heat sample solution, to achieve the effect that concentration and evaporation, although photo-thermal medium photo-thermal with higher mentioned above turns
Efficiency is changed, but the evaporation system efficiency based on gas-liquid interface free in microchannel is still lower, still there is very big room for improvement.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of micro-fluidic sample concentration device based on photo-thermal evaporation
And application method, to improve evaporation rate and evaporation stability.
In order to solve the above-mentioned technical problem, the technical scheme is that
A kind of micro-fluidic sample concentration device based on photo-thermal evaporation, including sample solution channel, it is characterised in that: described
It is provided with hole optical thermal evaporation layer above sample solution channel, is the evaporation place of device；The hole optical thermal evaporation layer
Top is provided with carrier gas passage, is provided with light source on the outside of the hole optical thermal evaporation layer；The hole optical thermal evaporation layer is by parent
Water hole optical thermal medium layer and hydrophobic gas diffusion layer composition；The hydrophilic porous photo-thermal dielectric layer generates liquid capillary pumped
Power makes sample solution enter hydrophilic porous photo-thermal dielectric layer, meanwhile, the incident light that hydrophilic porous photo-thermal dielectric layer absorbs light source produces
Third contact of a total solar or lunar eclipse fuel factor makes sample solution heat absorption evaporation generate gas, and then realizes the evaporation and concentration to sample solution；The hydrophobic gas
Body diffused layer is hydrophobic structure, is located at hydrophilic porous photo-thermal dielectric layer top, and hydrophobic gas diffusion layer is hydrophilic porous to allow
Photo-thermal dielectric layer is evaporated the gas generated in concentration process to sample solution and passes through, but it is logical to obstruct liquid phase sample solution simultaneously
It crosses, to realize gas-liquid separation, prevents gas from blocking runner, and then improve the efficiency that single unit system is concentrated by evaporation；The carrier gas is logical
Road has gas microchannel structure, and the import of the gas microchannel is connected to the gas outlet of purge gas storage device, the gas
The outlet of microchannel is connected to the air inlet of external device (ED)；It is passed through purge gas by the import in gas microchannel, by purging
The gas that gas sweeps along sample solution to generate in evaporating concentration process leaves hole optical thermal evaporation layer, and gas molecule is made to pass through gas
The outlet of body microchannel is transported to external device (ED).
The present invention proposes that a kind of provided by porous media structure transports the power of sample solution, optothermal material by being absorbed into
It penetrates light and the micro-fluidic sample concentration device for the energy that sample solution is concentrated by evaporation is provided.The present invention abandons general in microchannel
The convention for carrying out solution evaporation, the place that the hole optical thermal medium by being located at microchannel top is evaporated as solution, passes for steam
It is defeated to provide bigger access, improve the efficiency of vapor transmission in evaporation process；And by hydrophobic gas diffusion layer to gas-liquid two
It is mutually separated, effectively improves disengagement area, while solving the problems, such as that free gas-liquid interface is uncontrollable, it is steady to improve evaporation
It is qualitative；Hydrophilic porous photo-thermal dielectric layer effectively enhances evaporation process in microchannel, solution enter after hydrophilic porous dielectric layer
Photo-thermal effect acts on lower evaporation rate and can greatly improve.
The preferred embodiment of micro-fluidic sample concentration device according to the present invention based on photo-thermal evaporation, the sample are molten
Liquid channel has microchannel structure, is made using dimethyl silicone polymer, rubber or resinous material using soft lithography.
The preferred embodiment of micro-fluidic sample concentration device according to the present invention based on photo-thermal evaporation is described hydrophilic more
Hole photo-thermal dielectric layer is by foamy carbon as matrix.
Second technical solution of the invention is:
The application method of micro-fluidic sample concentration device based on photo-thermal evaporation, it is characterised in that: the enrichment facility includes
Sample solution channel is provided with hole optical thermal evaporation layer above the sample solution channel, the hole optical thermal evaporation layer
Top is provided with carrier gas passage, is provided with light source on the outside of the hole optical thermal evaporation layer；The hole optical thermal evaporation layer is by parent
Water hole optical thermal medium layer and hydrophobic gas diffusion layer composition；The hydrophobic gas diffusion layer is hydrophobic structure, is located at hydrophilic more
Hole photo-thermal dielectric layer top；The carrier gas passage has gas microchannel structure, the import and purge gas of the gas microchannel
The gas outlet of storage device is connected to, and the outlet of the gas microchannel is connected to the air inlet of external device (ED).
Steadily sample solution is passed through in sample solution channel using micro-injection pump, the hydrophilic porous photo-thermal medium
Layer generates capillary suction force to liquid, and sample solution is made to enter hydrophilic porous photo-thermal dielectric layer；Adjusting injection again, this is hydrophilic porous
The light source of photo-thermal dielectric layer adjusts the wavelength of incident light according to the solution caloric receptivity for wishing to reach, and then keeps this hydrophilic porous
Photo-thermal dielectric layer generates photo-thermal effect, makes the sample solution heat absorption evaporation into hydrophilic porous photo-thermal dielectric layer.
Sample solution carries out the gas generated in heat absorption evaporation process and enters hydrophobic gas by hydrophilic porous photo-thermal dielectric layer
Body diffused layer, but unevaporated sample solution is stayed in the hydrophilic porous photo-thermal dielectric layer by hydrophobic gas diffusion layer barrier；
Purge gas storage device is passed through purge gas, such as nitrogen etc. to the import of gas microchannel, due in gas microchannel
There is gas circulation, cause pressure reduction in gas microchannel, makes the gas in hydrophobic gas diffusion layer is spontaneous to enter carrier gas passage,
The gas that purge gas sweeps along sample solution to generate in evaporating concentration process leaves hole optical thermal evaporation layer, and gas molecule passes through
The outlet of gas microchannel is transported to external device (ED).
The preferred embodiment of the application method of micro-fluidic sample concentration device according to the present invention based on photo-thermal evaporation,
The sample solution channel has microchannel structure, utilizes soft lithographic using dimethyl silicone polymer, rubber or resinous material
Technology is made.
The preferred embodiment of the application method of micro-fluidic sample concentration device according to the present invention based on photo-thermal evaporation,
The hydrophilic porous photo-thermal dielectric layer is by foamy carbon as matrix.
The beneficial effect of micro-fluidic sample concentration device and application method of the present invention based on photo-thermal evaporation is: this
The place that invention is evaporated by the hole optical thermal medium for being located at microchannel top as solution provides bigger lead to for vapor transmission
Road improves the efficiency of vapor transmission in evaporation process；And gas-liquid two-phase is separated by hydrophobic gas diffusion layer, effectively mention
Disengagement area has been risen, while having solved the problems, such as that free gas-liquid interface is uncontrollable, has improved evaporation stability；Hydrophilic porous photo-thermal
Dielectric layer effectively enhances evaporation process in microchannel, and solution evaporates under photo-thermal effect effect after entering hydrophilic porous dielectric layer
Rate can greatly improve, and can be widely used in the fields such as crystal purification, pharmaceutical synthesis, food engineering.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the micro-fluidic sample concentration device of the present invention based on photo-thermal evaporation.
Fig. 2 is the structural schematic diagram of hole optical thermal evaporation layer 2 of the present invention.
Specific embodiment
Below with reference to test example and specific embodiment, the present invention is described in further detail.But this should not be understood
It is all that this is belonged to based on the technology that the content of present invention is realized for the scope of the above subject matter of the present invention is limited to the following embodiments
The range of invention.
Embodiment 1: referring to Fig. 1 and Fig. 2, a kind of micro-fluidic sample concentration device based on photo-thermal evaporation, including sample are molten
Liquid channel 1 is substrate, and the top in the sample solution channel 1 is provided with hole optical thermal evaporation layer 2, the hole optical thermal evaporation layer
2 top is provided with carrier gas passage 3, and the outside of the hole optical thermal evaporation layer 2 is provided with light source 4；The hole optical thermal evaporation
Layer 2 is made of hydrophilic porous photo-thermal dielectric layer 5 and hydrophobic gas diffusion layer 6；The hydrophilic porous photo-thermal dielectric layer 5 produces liquid
Raw capillary suction force makes sample solution enter hydrophilic porous photo-thermal dielectric layer 5, meanwhile, hydrophilic porous photo-thermal dielectric layer 5 absorbs light
The incident light in source 4, which generates photo-thermal effect, makes sample solution heat absorption evaporation generate gas, and then realizes dense to the evaporation of sample solution
Contracting；The hydrophobic gas diffusion layer 6 is hydrophobic structure, is located at hydrophilic porous 5 top of photo-thermal dielectric layer, hydrophobic gas diffusion layer 6
Pass through to allow hydrophilic porous photo-thermal dielectric layer 5 to be evaporated the gas generated in concentration process to sample solution, but simultaneously
Barrier liquid phase sample solution passes through, and to realize gas-liquid separation, prevents gas from blocking runner, and then improve single unit system and be concentrated by evaporation
Efficiency；The carrier gas passage 3 has gas microchannel structure, import and the purge gas storage device of the gas microchannel
Gas outlet connection, the outlet of the gas microchannel is connected to the air inlet of external device (ED)；It is logical by the import in gas microchannel
Enter purge gas, hole optical thermal evaporation layer is left by the gas that purge gas sweeps along sample solution to generate in evaporating concentration process
2, so that gas molecule is transported to external device (ED) by the outlet of gas microchannel.
In a particular embodiment, the sample solution channel 1 has microchannel structure, using dimethyl silicone polymer, rubber
Glue or resinous material are made using soft lithography.The microchannel surface should have a biggish channel width and reasonable depth
Degree, and the design of exposure mask can be modified according to the actual situation, to produce different microchannels to adapt to different solutions sample
Demand.In practical operation, steadily sample solution is passed through in microchannel using micro-injection pump, guarantees that solution example is continuously steady
Surely pass through, and can fit together with the porous media material on top.
The hydrophilic porous photo-thermal dielectric layer 5 is by foamy carbon as matrix.
Common microchannel is formed by substrate and cover board Direct Bonding, there is preferable leakproofness.Substrate, that is, sample of the present invention
Product solution channel 1, cover board, that is, carrier gas passage 3.The hole optical thermal evaporation layer is between substrate and cover board, this is to device
Leakproofness proposes biggish challenge.In the manufacturing process of device, the hole optical thermal evaporation layer for needing to make first is placed in pre-
In the substrate first processed, uncured PDMS is coated on evaporation layer edge and is sealed, then cover board is completed postposition
It is bonded under specific environment, to guarantee the leakproofness of device.
The course of work of the invention is:
The hydrophilic porous work of photo-thermal dielectric layer 5 is in solid liquid interface, since its pore structure can be in sample solution channel 1
Solution generate swabbing action, under the driving of capillary force, a part of sample solution can enter hole optical thermal evaporation layer 2 in.With
The light source 4 for injecting the evaporation layer is adjusted afterwards, the wavelength of incident light is adjusted according to the solution caloric receptivity for wishing to reach, and then make
The optothermal material of the evaporation layer generates photo-thermal effect, makes the sample solution heat absorption evaporation into evaporation layer.At the same time, it is believed that
The sample solution for remaining in sample solution channel 1 and the sample solution for having entered evaporation layer are a continuous fluids, work as evaporation layer
When the solution heat absorption evaporation of interior solution, the relative amount of solvent is then reduced, and can make sample by the motive force that concentration gradient generates
Solvent molecule in solution channel 1 spontaneously enters in evaporation layer, and then realization one is continuously to the evaporation of sample solution
Journey.The hydrophobic gas diffusion layer 6 is located at hydrophilic porous 5 top of photo-thermal dielectric layer, generally hydrophobic structure, main function
It is to provide circulation passage for the gas of generation, and prevent sample solution from leaving 5 hydrophilic porous photo-thermal dielectric layers and move upwards.In reality
It in the operation of border, because of this layer of hydrophobic structure, is only capable of passing the gas through and having completely cut off liquid solution, and using above it
Carrier gas passage 3 is passed through purge gas, helps gas molecule to leave evaporation layer, helps that gas molecule is transported to outside device rapidly
Portion, and then improve evaporation efficiency.
The application method of embodiment 2, the micro-fluidic sample concentration device evaporated based on photo-thermal, which includes sample
Solution channel 1, the top in the sample solution channel 1 are provided with hole optical thermal evaporation layer 2, the hole optical thermal evaporation layer 2
Top is provided with carrier gas passage 3, and the outside of the hole optical thermal evaporation layer 2 is provided with light source 4；The hole optical thermal evaporation layer 2
It is made of hydrophilic porous photo-thermal dielectric layer 5 and hydrophobic gas diffusion layer 6；The hydrophobic gas diffusion layer 6 is hydrophobic structure, is located at
Hydrophilic porous 5 top of photo-thermal dielectric layer；The carrier gas passage 3 have gas microchannel structure, the import of the gas microchannel with
The gas outlet of purge gas storage device is connected to, and the outlet of the gas microchannel is connected to the air inlet of external device (ED).
Steadily sample solution is passed through in sample solution channel 1 using micro-injection pump, the hydrophilic porous photo-thermal is situated between
5 pairs of liquid of matter layer generate capillary suction force, and sample solution is made to enter hydrophilic porous photo-thermal dielectric layer 5；Adjusting injection again, this is hydrophilic
The light source of hole optical thermal medium layer 5 adjusts the wavelength of incident light according to the solution caloric receptivity for wishing to reach, and then keeps this hydrophilic
Hole optical thermal medium layer 5 generates photo-thermal effect, makes the sample solution heat absorption evaporation into hydrophilic porous photo-thermal dielectric layer 5.
Sample solution carries out the gas generated in heat absorption evaporation process and enters hydrophobic gas by hydrophilic porous photo-thermal dielectric layer 5
Body diffused layer 6, but unevaporated sample solution stays in the hydrophilic porous photo-thermal dielectric layer 5 by the barrier of hydrophobic gas diffusion layer 6
Interior, purge gas storage device is passed through purge gas, such as nitrogen etc. to the import of gas microchannel, due in gas microchannel
Inside there is gas circulation, cause pressure reduction in gas microchannel, keeps the gas in hydrophobic gas diffusion layer 6 spontaneous logical into carrier gas
Road, the gas that purge gas sweeps along sample solution to generate in evaporating concentration process leave hole optical thermal evaporation layer 2, gas molecule
External device (ED) is transported to by the outlet of gas microchannel.
In a particular embodiment, the sample solution channel 1 has microchannel structure, using dimethyl silicone polymer, rubber
Glue or resinous material pour.
The hydrophilic porous photo-thermal dielectric layer 5 is by foamy carbon as matrix.
Embodiment 3: the micro-fluidic sample concentration device based on photo-thermal evaporation can be made as follows:
A, sample solution channel 1 is processed with carrier gas passage 3: choosing two block-shaped polydimethylsiloxanes identical with size
Alkane, rubber or resin elastic-like material pass through soft lithography respectively as the matrix in sample solution channel 1 and carrier gas passage 3
Processing solution channel and gas microchannel in matrix respectively.
B, the production of hole optical thermal evaporation layer 2:
B1, by liquefied carbolic acid, calcium lignosulfonate, sodium hydroxide 39.2:4.4:1 in mass ratio after evenly mixing at 80 DEG C
30min is heated under constant temperature, then 30% formalin is added in the 1:1.27 in mass ratio on the basis of mixture quality,
Vacuum dehydration 30min, obtains resol resins under temperature 50 C, pressure 0.1MPa.
B2, resol resins, lauryl alcohol, each 1g of dodecyl sodium sulfate are added in 50mL deionized water, at 60 DEG C
It is cooled to room temperature after lower heating 30min, at room temperature with 1200rpm magnetic agitation 8min, obtains foaming agent.
B3,3min is mixed in resol resins, foaming agent, isocyanates 2.2:2.7:1 in mass ratio, is packed into mould
30min is stood in tool obtains porous resin template.
B4, it will be heat-treated 3h at a temperature of porous resin template in a reducing atmosphere 800-1200 DEG C, obtains porous foam carbon；
By foaming agent in preparation process and resol resins ratio regulation and control heat treatment time to foamy carbon pore-size into
Row regulation, using porous foam carbon obtained as the substrate of hydrophilic porous photo-thermal dielectric layer, by porous foam carbon 1M sulfuric acid with
30% hydrogenperoxide steam generator impregnates 1 hour, impregnates 2 hours in volume fraction for stirring in 4% nitric acid after dry, inert blowing gas
After sweeping drying, in N2Plasma environment is handled 30 minutes, obtains hydrophilic porous photo-thermal medium.
B5, hydrophobic gas diffusion layer is loaded on hydrophilic porous photo-thermal medium:
The polytetrafluoroethylene (PTFE) Polytetrafluoroethylene for being 60% by concentration, PTFE emulsion ultrasonic vibration 30 divide
Clock, even application are 2mg/cm 2 in hydrophilic porous layer side, control carrying capacity, heat 1 hour, are made at 350 DEG C after spraying
Hydrophobic gas diffusion layer is so far completed hole optical thermal evaporation layer 2 and is prepared.
C, it assembles:
The hole optical thermal evaporation layer 2 made is placed in the substrate processed, there is the one of solution microchannel in substrate
Upwardly, the hole optical thermal evaporation to complete is placed on base, uncured PDMS is coated on evaporation layer edge, with
Device is handled 30 minutes in oxygen plasma environment and is bonded by rear cover upper cover plate, adds device at 120 DEG C after bonding
Heat 2 hours, to guarantee the leakproofness of PDMS bond strength and device.
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, although with reference to the foregoing embodiments
Invention is explained in detail, for those skilled in the art still can be to skill documented by foregoing embodiments
Art scheme is modified or equivalent replacement of some of the technical features.All within the spirits and principles of the present invention,
Any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of micro-fluidic sample concentration device based on photo-thermal evaporation, including sample solution channel (1), it is characterised in that: institute
It states and is provided with hole optical thermal evaporation layer (2) above sample solution channel (1), set above the hole optical thermal evaporation layer (2)
It is equipped with carrier gas passage (3), is provided with light source (4) on the outside of the hole optical thermal evaporation layer (2)；The hole optical thermal evaporation layer
(2) it is made of hydrophilic porous photo-thermal dielectric layer (5) and hydrophobic gas diffusion layer (6)；The hydrophilic porous photo-thermal dielectric layer (5) is right
Liquid generates capillary suction force, and sample solution is made to enter hydrophilic porous photo-thermal dielectric layer (5), meanwhile, hydrophilic porous photo-thermal medium
The incident light that layer (5) absorbs light source (4), which generates photo-thermal effect, makes sample solution heat absorption evaporation generate gas, and then realizes to sample
The evaporation and concentration of solution；The hydrophobic gas diffusion layer (6) is hydrophobic structure, is located at hydrophilic porous photo-thermal dielectric layer (5) top,
Hydrophobic gas diffusion layer (6) is produced to allow hydrophilic porous photo-thermal dielectric layer (5) to be evaporated in concentration process sample solution
Raw gas passes through, but obstructs liquid phase sample solution simultaneously and pass through；The carrier gas passage (3) has gas microchannel structure, should
The import of gas microchannel is connected to the gas outlet of purge gas storage device, the outlet of the gas microchannel and external device (ED)
Air inlet connection；It is passed through purge gas by the import in gas microchannel, sweeps along sample solution dense in evaporation by purge gas
The gas generated in compression process leaves hole optical thermal evaporation layer (2), is transported to gas molecule by the outlet of gas microchannel
External device (ED).
2. the micro-fluidic sample concentration device according to claim 1 based on photo-thermal evaporation, it is characterised in that: the sample
Solution channel (1) has microchannel structure, is made of dimethyl silicone polymer, rubber or resinous material.
3. the micro-fluidic sample concentration device according to claim 1 or 2 based on photo-thermal evaporation, it is characterised in that: described
Hydrophilic porous photo-thermal dielectric layer (5) is by foamy carbon as matrix.
4. the application method of the micro-fluidic sample concentration device based on photo-thermal evaporation, it is characterised in that: the enrichment facility includes sample
Product solution channel (1), hole optical thermal evaporation layer (2) is provided with above the sample solution channel (1), and the porous photo-thermal steams
It is provided with carrier gas passage (3) above hair layer (2), is provided with light source (4) on the outside of the hole optical thermal evaporation layer (2)；It is described
Hole optical thermal evaporation layer (2) is made of hydrophilic porous photo-thermal dielectric layer (5) and hydrophobic gas diffusion layer (6)；The hydrophobic gas
Diffusion layer (6) is hydrophobic structure, is located at hydrophilic porous photo-thermal dielectric layer (5) top；The carrier gas passage (3) has gas micro- logical
The import of road structure, the gas microchannel is connected to the gas outlet of purge gas storage device, the outlet of the gas microchannel with
The air inlet of external device (ED) is connected to；
Sample solution is passed through in sample solution channel (1) using micro-injection pump, the hydrophilic porous photo-thermal dielectric layer (5) is right
Liquid generates capillary suction force, and sample solution is made to enter hydrophilic porous photo-thermal dielectric layer (5)；It adjusts again and injects the hydrophilic porous light
The light source of thermal medium layer (5) adjusts the wavelength of incident light according to the solution caloric receptivity for wishing to reach, and then keeps this hydrophilic porous
Photo-thermal dielectric layer (5) generates photo-thermal effect, makes the sample solution heat absorption evaporation into hydrophilic porous photo-thermal dielectric layer (5)；
Sample solution carries out the gas generated in heat absorption evaporation process and enters hydrophobic gas by hydrophilic porous photo-thermal dielectric layer (5)
Diffusion layer (6), but unevaporated sample solution stays in the hydrophilic porous photo-thermal dielectric layer by hydrophobic gas diffusion layer (6) barrier
(5) in, purge gas storage device is passed through purge gas to the import of gas microchannel, makes in hydrophobic gas diffusion layer (6)
Gas is spontaneous to enter carrier gas passage, and the gas that purge gas sweeps along sample solution to generate in evaporating concentration process leaves hole optical
Thermal evaporation layer (2), gas molecule are transported to external device (ED) by the outlet of gas microchannel.
5. the application method of the micro-fluidic sample concentration device according to claim 4 based on photo-thermal evaporation, feature exist
In:
The sample solution channel (1) has microchannel structure, using dimethyl silicone polymer, rubber or resinous material system
At.
6. the application method of the micro-fluidic sample concentration device according to claim 4 based on photo-thermal evaporation, feature exist
In: the hydrophilic porous photo-thermal dielectric layer (5) is by foamy carbon as matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910516415.7A CN110208073B (en) | 2019-06-14 | 2019-06-14 | Micro-fluidic sample concentration device based on photo-thermal evaporation and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910516415.7A CN110208073B (en) | 2019-06-14 | 2019-06-14 | Micro-fluidic sample concentration device based on photo-thermal evaporation and using method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110208073A true CN110208073A (en) | 2019-09-06 |
| CN110208073B CN110208073B (en) | 2021-03-16 |
Family
ID=67792789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910516415.7A Active CN110208073B (en) | 2019-06-14 | 2019-06-14 | Micro-fluidic sample concentration device based on photo-thermal evaporation and using method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110208073B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112707391A (en) * | 2021-01-04 | 2021-04-27 | 东华大学 | Self-water-supply type light hot water evaporation device based on composite hydrogel |
| CN114229937A (en) * | 2021-12-20 | 2022-03-25 | 中国矿业大学 | High salinity mine water desalination device based on interface photothermal evaporation |
| CN114308153A (en) * | 2021-12-17 | 2022-04-12 | 华南农业大学 | Solid-phase extraction micro-fluidic chip for detecting nafil substances and system for detecting nafil substances |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050186118A1 (en) * | 2004-02-25 | 2005-08-25 | Bishop David J. | Micro-channel chemical concentrator |
| CN103849674A (en) * | 2012-11-30 | 2014-06-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | Hydrophilic and hydrophobic substrate-based micro-liquid phase reaction method |
| CN107879405A (en) * | 2017-10-26 | 2018-04-06 | 西安交通大学 | A kind of solar energy water evaporation purifying and decomposer |
-
2019
- 2019-06-14 CN CN201910516415.7A patent/CN110208073B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050186118A1 (en) * | 2004-02-25 | 2005-08-25 | Bishop David J. | Micro-channel chemical concentrator |
| CN103849674A (en) * | 2012-11-30 | 2014-06-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | Hydrophilic and hydrophobic substrate-based micro-liquid phase reaction method |
| CN107879405A (en) * | 2017-10-26 | 2018-04-06 | 西安交通大学 | A kind of solar energy water evaporation purifying and decomposer |
Non-Patent Citations (2)
| Title |
|---|
| 张家埭: "《碳材料工程基础》", 30 April 1992, 冶金工业出版社 * |
| 白光清: "《光电领域专利审查研究》", 30 September 2017, 知识产权出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112707391A (en) * | 2021-01-04 | 2021-04-27 | 东华大学 | Self-water-supply type light hot water evaporation device based on composite hydrogel |
| CN114308153A (en) * | 2021-12-17 | 2022-04-12 | 华南农业大学 | Solid-phase extraction micro-fluidic chip for detecting nafil substances and system for detecting nafil substances |
| CN114229937A (en) * | 2021-12-20 | 2022-03-25 | 中国矿业大学 | High salinity mine water desalination device based on interface photothermal evaporation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110208073B (en) | 2021-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110208073A (en) | Micro-fluidic sample concentration device and application method based on photo-thermal evaporation | |
| Zhang et al. | PMMA/PDMS valves and pumps for disposable microfluidics | |
| US20020075363A1 (en) | Air flow regulation in microfluidic circuits for pressure control and gaseous exchange | |
| Murad et al. | A computer simulation of the classic experiment on osmosis and osmotic pressure | |
| Bacchin et al. | Microfluidic evaporation, pervaporation, and osmosis: from passive pumping to solute concentration | |
| JP2007136280A (en) | Microfractionating device and fractionating process | |
| EP3055055B1 (en) | Method for stirring at least one liquid | |
| Wang et al. | Anisotropic Janus Si nanopillar arrays as a microfluidic one-way valve for gas–liquid separation | |
| Jeong et al. | On the role of oxygen in fabricating microfluidic channels with ultraviolet curable materials | |
| CN110804650A (en) | Circulating type digital PCR method, circulating system, digital PCR chip and preparation method thereof | |
| Ye et al. | Open-channel microfluidic chip based on shape memory polymer for controllable liquid transport | |
| US20050196328A1 (en) | Hydraulic device for the thermo-pneumatic isolation and optional agitation of the contents of an operative cavity | |
| He et al. | Laser assisted microfluidic membrane evaporator for sample crystallization separation | |
| CN107061863B (en) | It is a kind of to generate the micro-valve system for controlling parked bubble and generate control method | |
| JP2006198542A (en) | Microrectification apparatus and rectification method | |
| CN113694974B (en) | Photo-thermal induced bubble assisted device for moving liquid drops on solid surface and using method | |
| CN104549590A (en) | Technology for modifying inner wall of micro-channel in metal micro-fluidic chip | |
| CN108591610B (en) | Microfluidic system, micro valve and control method | |
| CN109647546A (en) | A kind of aqueous two-phase drop chip of pneumatic operated valve auxiliary | |
| Seo et al. | Gas transport mechanisms through gas-permeable membranes in microfluidics: A perspective | |
| CN108031500B (en) | Hydrophobic modification method for micro-channel in micro-fluidic chip | |
| CN104736247B (en) | Fluid control in microfluidic device | |
| Hibara et al. | Integrated fluidic systems on a nanometer scale and the study on behavior of liquids in small confinement | |
| Xie et al. | Functional microfluidics: theory, microfabrication, and applications | |
| CN112844499A (en) | Micro-fluidic chip for preparing polyelectrolyte microcapsules by using aqueous two-phase system and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |