CN110201010A - A kind of beet total saponin extracts and preparation method thereof - Google Patents

A kind of beet total saponin extracts and preparation method thereof Download PDF

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Publication number
CN110201010A
CN110201010A CN201910416446.5A CN201910416446A CN110201010A CN 110201010 A CN110201010 A CN 110201010A CN 201910416446 A CN201910416446 A CN 201910416446A CN 110201010 A CN110201010 A CN 110201010A
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China
Prior art keywords
beet
total saponin
extracts
saponin extracts
added
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Pending
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CN201910416446.5A
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Chinese (zh)
Inventor
李瑶函
张建宾
田景奎
朱玮
王若宜
李守信
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Changshu Qiushi Technology Co Ltd
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Changshu Qiushi Technology Co Ltd
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Priority to CN201910416446.5A priority Critical patent/CN110201010A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/06Antihyperlipidemics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/55Liquid-liquid separation; Phase separation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Mycology (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Nutrition Science (AREA)
  • Emergency Medicine (AREA)
  • Endocrinology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Epidemiology (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A kind of invention category technical field of food biotechnology, it is related to a kind of beet total saponin extracts and preparation method thereof, it is that will take beet, it gives as one thinks fit cataclasm, water or alcohol-water is added to extract, quantity of solvent, extraction time and extraction time is added in control, combined extract filters while hot, filtrate recycling design, the concentrate is added in the large pore resin absorption column pre-processed, first with the washing of 2~10 times of resin volumes, then with 4~12 times of resin volumes, the ethanol elution that concentration is 20~95%, eluent is collected, it is concentrated and dried after recycling ethyl alcohol, obtains beet total saponin extracts.The present invention is suitble to industrialized production to prepare beet total saponin extracts, provides broader approach for the comprehensive development and utilization of beet.

Description

A kind of beet total saponin extracts and preparation method thereof
Technical field
The present invention provides beet total saponin extracts and its preparation methods, belong to technical field of food biotechnology.
Background technique
Beet (scientific name: Beta vulgaris L.) is chenopod, other than sugar rich in, also containing containing beet Alkali, vitamin A, vitamin C and saponins compound.More than 900 ten thousand tons of China's beet annual output, be main sugar crop it One.Beet is in addition to for refining sugar, by-product comprehensive utilization value with higher simultaneously, if molasses are through everfermentation, or it is logical Chemical method processing is crossed, methanol, ethyl alcohol, butanol, glycerol, monosodium glutamate and acetone etc. can be produced.Beet saponin(e has according to the literature Significant hypoglycemic effect is able to suppress the absorption of sugar, and saponins compound sufficiently develops and uses not yet.
Summary of the invention
In order to overcome the deficiencies of the prior art, it the present invention provides a kind of beet total saponin extracts and its preparation method, also provides Using it as the food and health food of auxiliary adjustment blood glucose made from raw material and adjusting blood lipid, blood glucose and blood can be effectively improved The metabolism of rouge.
The invention adopts the following technical scheme:
A kind of beet total saponin extracts, it is characterised in that: beet total saponin extracts are Li Ke Beta beet (Beta Vulgaris L.) it is middle extracting acquisition, total saponin content is 5%~100%;It is obtained by following methods:
(1) it extracts: taking beet, give as one thinks fit cataclasm, add water or alcohol-water to extract, control is added quantity of solvent, extraction time and mentions Take number;
(2) it separates: merging the extracting solution under (1) item, filter while hot, filtrate recycling design, which is added in advance In the large pore resin absorption column first handled well, first with the washing of 2~10 times of resin volumes, then with 4~12 times of resin volumes, concentration For 20~95% ethanol elution, eluent is collected, is concentrated and dried after recycling ethyl alcohol, obtains beet total saponin extracts.
Wherein step (1) described beet is the root of beet.
Wherein step (1) described beet can also be the leftover bits and pieces of beet sugar manufacture, including extracting the later megasse of sugar, system Filter mud after sugar.
As a preferred embodiment of the above technical solution, the solvent for use of step (1) is 30-90% ethyl alcohol, and quantity of solvent is beet amount 6~12 times, extraction time be 1~3 hour, extraction time be 1~3 time.
As a preferred embodiment of the above technical solution, macroporous absorbent resin described in step (2) be D101, AB-8, DM130 or Extract saponin(e resin.
As a preferred embodiment of the above technical solution, elution process described in step (2) is first with the water of 2~6 times of resin volumes Wash, then with 2~6 times of resin volumes, concentration be 50~80% ethanol elution.
Beet total saponin extracts of the present invention adjust answering in blood glucose and serum regulating drug and food in preparation With.
The present invention also provides with carrier acceptable on beet total saponin extracts of the invention and food or excipient The preparation of preparation.These preparations select following dosage forms: solution, suspension, emulsion, tablet, capsule, granule, pill, ingot Agent oral solution, sublingual tablet, effervescent tablet etc..
The present invention contains the pharmaceutical preparation of beet total saponin extracts, can also be optionally containing food when preparing pharmaceutical preparation Acceptable carrier, carrier come from product: cosolvent, solubilizer, suspending agent, emulsifier, surfactant, corrigent etc..
Provided by the invention by food made from raw material or health food of beet total saponin extracts not only includes folk prescription Preparation further includes that food or health food of the beet total saponin extracts as active constituent are added in compound preparation.
The beneficial effects of the present invention are: a kind of preparation method of the beet total saponin extracts provided, simple process is easy It is practical, extend the application value of beet.
Specific embodiment
It is that the present invention is further described in more detail below by way of specific embodiment, but not to the scope of the present invention structure At any restrictions.
Embodiment 1:
(1) beet 3kg is taken, gives as one thinks fit cataclasm, is added 24kg water boiling and extraction 2 times, every time 2 hours.
(2) merging filtrate filters while hot, and being concentrated into volume is 3L, is added on processed D101 macroporous resin column, column Bed volume 3L, first use 12L water elution, then use 6L95% ethanol elution, eluent recycle ethyl alcohol after be concentrated and dried, obtain the total soap of beet Glucoside extract 7.6g, obtained beet total saponin content are 9.8%.
Embodiment 2:
(1) beet root 10kg, give as one thinks fit it is cataclasm, add 80% ethyl alcohol 60L extract 3 times, every time 1 hour.
(2) merging filtrate filters while hot, and being concentrated into volume is 10L, is added on processed AB-8 macroporous resin column, column Bed volume 10L, first use 60L water elution, then use 60L30% ethanol elution, eluent recycle ethyl alcohol after be concentrated and dried, it is total to obtain beet Saponin extract 27g, obtained beet total saponin content are 25%.
Embodiment 3:
(1) megasse (drying) 5kg is taken, 50% ethyl alcohol 60L is added, is extracted 2 times, every time 1.5 hours.
(2) merging filtrate filters while hot, and being concentrated into volume is 5L, is added on processed DM130 macroporous resin column, column Bed volume 25L, first use 50L water elution, then use 75L70% ethanol elution, eluent recycle ethyl alcohol after be concentrated and dried, it is total to obtain beet Saponin extract 127g, obtained beet total saponin content are 75%.
Embodiment 4:
(1) beet filter mud 5kg is taken, 30% ethyl alcohol 30kg is added, is extracted 1 time, 3 hours.
(2) merging filtrate filters while hot, and being concentrated into volume is 5L, is added on processed extraction saponin(e resin column, column bed Volume 5L, first use 15L water elution, then use 20L50% ethanol elution, eluent recycle ethyl alcohol after be concentrated and dried, obtain the total soap of beet Glucoside extract 52g, obtained beet total saponin content are 55%.

Claims (7)

1. a kind of beet total saposins, it is characterised in that: mentioned from Li Ke beet platymiscium beet (Beta vulgaris L.) Acquisition is taken, total saponin content is 5%~100%;It is obtained by following methods:
(1) it extracts: taking beet, give as one thinks fit cataclasm, water or alcohol-water is added to extract, control is added quantity of solvent, extraction time and extracts time Number;
(2) it separates: merging the extracting solution under (1) item, filter while hot, filtrate recycling design, which is added on and is located in advance In the large pore resin absorption column managed, first with the washing of 2~10 times of resin volumes, then with 4~12 times of resin volumes, concentration 20 ~95% ethanol elution collects eluent, is concentrated and dried after recycling ethyl alcohol, obtains beet total saponin extracts.
2. beet total saponin extracts as described in claim 1, it is characterised in that: step (1) described beet is the root of beet.
3. beet total saponin extracts as described in claim 1, it is characterised in that: after step (1) described beet is beet sugar manufacture Leftover bits and pieces.
4. beet total saponin extracts as described in claim 1, it is characterised in that: step (1) extraction is that 30-90% is added Ethyl alcohol is 6~12 times of medicinal material amount;Extraction time is 1~3 hour;Extraction time is 1~3 time.
5. beet total saponin extracts as described in claim 1, it is characterised in that macroporous absorbent resin described in step (2) is D101, AB-8, DM130 extract saponin(e resin.
6. beet total saponin extracts as described in claim 1, it is characterised in that elution process described in step (2) is first with 2 The washing of~6 times of resin volumes, then with 2~6 times of resin volumes, concentration be 50~80% ethanol elution.
7. food and health care that the beet total saponin extracts as described in Claims 1 to 5 are used for auxiliary adjustment blood glucose and blood lipid Food.
CN201910416446.5A 2019-05-20 2019-05-20 A kind of beet total saponin extracts and preparation method thereof Pending CN110201010A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111467379A (en) * 2020-04-26 2020-07-31 苏州求是玉泉健康科技有限公司 Preparation method and application of beet extract

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
周奇文等: "《实用食品加工新技术精选.5》", 28 February 2003, 中国轻工业出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111467379A (en) * 2020-04-26 2020-07-31 苏州求是玉泉健康科技有限公司 Preparation method and application of beet extract
CN111467379B (en) * 2020-04-26 2021-09-14 苏州承瑞健康科技有限公司 Preparation method and application of beet extract

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