CN110196228A - Based on the micro- phosphatidase detection polarisation analyzer of no lens and detection method - Google Patents
Based on the micro- phosphatidase detection polarisation analyzer of no lens and detection method Download PDFInfo
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- CN110196228A CN110196228A CN201910383940.6A CN201910383940A CN110196228A CN 110196228 A CN110196228 A CN 110196228A CN 201910383940 A CN201910383940 A CN 201910383940A CN 110196228 A CN110196228 A CN 110196228A
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- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 38
- 238000003384 imaging method Methods 0.000 claims abstract description 28
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229910052802 copper Inorganic materials 0.000 claims abstract description 26
- 239000010949 copper Substances 0.000 claims abstract description 26
- 230000000007 visual effect Effects 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000003292 glue Substances 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000011521 glass Substances 0.000 claims description 12
- 230000008859 change Effects 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 9
- 229910021641 deionized water Inorganic materials 0.000 claims description 9
- 238000004873 anchoring Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- WSFMFXQNYPNYGG-UHFFFAOYSA-M dimethyl-octadecyl-(3-trimethoxysilylpropyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCC[Si](OC)(OC)OC WSFMFXQNYPNYGG-UHFFFAOYSA-M 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 241000252506 Characiformes Species 0.000 claims description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 5
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000004988 Nematic liquid crystal Substances 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 102100026918 Phospholipase A2 Human genes 0.000 description 2
- 101710096328 Phospholipase A2 Proteins 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000005212 4-Cyano-4'-pentylbiphenyl Substances 0.000 description 1
- HHPCNRKYVYWYAU-UHFFFAOYSA-N 4-cyano-4'-pentylbiphenyl Chemical group C1=CC(CCCCC)=CC=C1C1=CC=C(C#N)C=C1 HHPCNRKYVYWYAU-UHFFFAOYSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000879 optical micrograph Methods 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4788—Diffraction
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to micro-imaging technique fields, disclose a kind of micro- based on no lens phosphatidase detection polarisation analyzer and detection method, the phosphatidase detection polarisation analyzer includes the light source for being arranged successively setting from the bottom up, the micro-flow groove containing liquid crystalline sample and the cmos imaging sensor for image recording, the micro-flow groove include slot cover, slot bottom, positioned at the slide of miniflow trench bottom being anchored and on slide for loading the copper mesh of liquid crystal, slot cover and slot bottom are adsorbed with 90 ° of light polarizing films and 0 ° of light polarizing film respectively.The polarisation analyzer of the present invention has big visual field (FOV=24.4mm2), it is at low cost, it is portable, it is easy to operate, it can be realized the advantages such as real-time, quick detection.
Description
Technical field
The present invention relates to micro-imaging technique fields, inclined more particularly, to being detected based on the micro- phosphatidase of no lens
Light analyzer and detection method.
Background technique
Liquid crystal is widely used in all kinds of electronical displays due to having two kinds of properties of mobility and crystallographic simultaneously
In screen.Since liquid crystal has the characteristics that, by the sensitive intervening material as bio-sensing, to be applied to biology to extraneous environment sensitive
Medical domain.1998, nematic liquid crystal molecules were used in sense biomolecule and lead since it is bent to the order of arrangement
Domain.For technique because its specificity is good, without marking the features such as causes the extensive concern of scholar.Phosphatidase (PLA2) is a kind of
Important cell membrane enzyme, by interacting with intracellular phosphatide, to promote cell metabolism.Therefore it is led in biomedicine
Domain, phosphatidase (PLA2) is through the important test object frequently as certain diseases.It is existing measurement phosphatidase method have titration,
Colorimetric method, nuclear magnetic resonance spectrometry, Radio labeled method and fluorescence analysis etc..The more common method of phosphatide enzymatic determination is at present
Radio labeled method, but this method increases detection difficulty, while structure of phospholipid being be easy to cause to change, and reduces enzymatic activity.2003
Year, Abbort research group has reported a kind of new research method.This method is adsorbed on liquid crystal surfactant using phosphatide enzyme hydrolysis
Phosphatide causes liquid crystal molecular orientation to change, and passes through the brightness change of the micro- sem observation liquid crystal visual field of polarization optics, to realize
The detection of phosphatidase.This method can monitor distribution of the phosphatide on liquid crystal, understand the process of phosphatide enzyme hydrolysis phosphide.The party
Method is simple to the treatment process of sample, and intuitive to the observation of sample, simply, directly carries out brightness sight using petrographic microscope
It examines.But petrographic microscope have it is expensive, volume is big, it is not readily portable the disadvantages of.
Summary of the invention
The present invention is directed to overcome at least one of the above-mentioned prior art insufficient, a kind of phosphatide micro- based on no lens is provided
Enzyme detects polarisation analyzer, and the polarisation analyzer has big visual field (FOV=24.4mm2), it is at low cost, it is portable, it is easy to operate,
It can be realized the advantages such as real-time, quick detection.
Another object of the present invention is to provide the methods using phosphatidase polarisation analyzer detection phosphatidase.
The technical solution adopted by the present invention is as follows:
A kind of phosphatidase detection polarisation analyzer micro- based on no lens, the light including being arranged successively setting from the bottom up
Source, the micro-flow groove containing liquid crystalline sample and the cmos imaging sensor for image recording, the micro-flow groove include slot cover, slot
Bottom, the copper mesh for being used to load liquid crystal positioned at the slide of miniflow trench bottom being anchored and on slide, slot cover and slot bottom point
It is not adsorbed with 90 ° of light polarizing films and 0 ° of light polarizing film.
The working principle of the phosphatidase detection polarisation analyzer micro- based on no lens described herein is as follows: micro-flow groove
Liquid crystalline sample is carried, the sample for posting light polarizing film is placed directly on cmos imaging sensor, the light that light source issues penetrates sample
After diffraction occurs, the diffraction pattern of sample is recorded by cmos imaging sensor, that is, the variation of sample can be observed.
Preferably, the slot cover is equipped with micro-flow groove water inlet and micro-flow groove water outlet.
Preferably, the light source is the monochromatic LED lamp that peak wavelength is 450nm, power is 0.5W.
Preferably, 90 ° of light polarizing films, 0 ° of light polarizing film thickness be 0.1~0.5mm.Preferably, the cmos imaging
The pixel size of sensor is 2.2 μm, and pixel is 5,000,000 (1944 × 2592), and imaging sensing area is 24.4mm2。
Preferably, the distance between slot cover of the light source and micro-flow groove is 10~13cm.When light source and micro-flow groove away from
From it is too small when, the light source optical power that cmos imaging sensor receives is stronger, be easy to cause image overexposure.
Preferably, the distance between the slot bottom of the micro-flow groove and cmos imaging sensor are 0.5~3mm.Influence shooting
The main reason for image definition is micro-flow groove apart from the distance between cmos imaging sensor, and distance between the two is smaller,
Cmos imaging sensor it is better at the clarity of image, it is contemplated that the limitation of system itself, the present invention in micro-flow groove slot bottom
Distance to cmos sensor is about 0.5~3mm preferable.
Preferably, the slide is fixed on the center of slot bottom by ultraviolet glue, and the copper mesh is fixed by ultraviolet glue
In the center of slide.
Preferably, the copper mesh is the copper mesh in 285 μm of 75 mesh aperture.
Using the method for the phosphatidase polarisation analyzer detection phosphatidase, include the following steps:
S1, it slide is subjected to anchoring is placed in the slot bottom of micro-flow groove, then fix a copper mesh on slide, and coat on copper mesh
Liquid crystal, then the slot bottom of micro-flow groove and slot cover are sealed；
S2, micro-flow groove is placed in above cmos imaging sensor at 0.5~3mm, using small needle tube successively by TBS buffer, phosphorus
Rouge, phosphatidase inject micro-flow groove from micro-flow groove water inlet, observe the brightness change of liquid crystal in visual field, that is, detect containing for phosphatidase
Amount.
Preferably, process slide being anchored are as follows:
S11. the slide of one piece of 1cm × 1cm after cleaning is placed in glass dish, and pours into Piranha quarter into glass dish
Liquid is lost, until slide is submerged, slide is impregnated into 25~35min；
S12. the slide after impregnating is rinsed with deionized water；
S13. the flushed slide of deionized water is placed into the DMOAP aqueous solution that volumetric concentration is 0.5%~1% and impregnates 5
~15min, then cleaned with deionized water, it is then placed into baking oven and is heated to 75~85 DEG C, the slide being anchored.
It is vertical on slide in order to ensure liquid crystal that slide, which needs the purpose for first passing through anchoring treatment in advance, in the technical program
Orientation, so that visual field is dark state, after TBS buffer has been added dropwise, TBS buffer can induce liquid crystal in horizontal alignment, make
The liquid crystal obtained in visual field brightens, and after adsorbing phosphatide on nematic liquid crystal film, phosphatide can induce the perpendicular orientation of liquid crystal, visual field
Interior liquid crystal is again dimmed, after phosphatide enzyme solutions have been added dropwise, phosphatidase hydrolytic phosphatide, and induce liquid crystal in horizontal alignment, so that
Liquid crystal in visual field brightens.
Further, step S1 are as follows:
S14., the slide being anchored is placed in the slot bottom of micro-flow groove with tweezers, it is then ultraviolet in upper 1~2 drop of the diagonal angle drop of slide
Glue uses ultraviolet radiation-curable ultraviolet glue；
S15. copper mesh is placed in the surface of glass slide in micro-flow groove, and in 1 drop purple light glue of surface of glass slide drop, is shifted onto copper mesh with tweezers
With the just accessible position of purple light glue；
S16. solidify ultraviolet glue using ultraviolet light, 1~2 dropping liquid crystalline substance is uniformly coated in copper mesh, it will be extra with capillary
Liquid crystal is drawn completely, and micro-flow groove slot cover is covered；
S17. the slot bottom of micro-flow groove and slot cover are sealed with purple light glue.
Preferably, liquid crystal used is nematic liquid crystal 4- cyano -4'- pentylbiphenyl.
Compared with prior art, the invention has the benefit that compared with traditional optical microphotograph polariscope, institute of the present invention
The detection polarisation analyzer stated does not need any optical lens and is focused, therefore can be realized in real time, is quickly imaged, also
It can integrate as the device of a small size, there is portability, simultaneously as optical element used is less, cost of consumption is low,
It is easy to operate.The present invention overcomes all disadvantages for using traditional petrographic microscope to carry out phosphatidase detection, in addition to this, also
With big visual field, can be carried out quickly, in real time the advantages such as imaging.
Detailed description of the invention
Fig. 1 is that the phosphatidase micro- based on no lens of the invention detects polarisation analyzer structural schematic diagram.
Fig. 2 is the micro-flow groove schematic diagram that liquid crystalline sample is housed in the present invention.
Liquid crystal in visual field when Fig. 3 adds the addition different solutions observed in the case of 0 ° and 90 ° polarization for 5x Zeiss microscope
Brightness change situation map.
Fig. 4 is the brightness change situation map using liquid crystal in visual field when present invention observation when addition different solutions.
Detailed description of the invention: 1, light source；2, micro-flow groove water inlet；3, micro-flow groove water outlet；4,90 ° of light polarizing films；5, miniflow mortise；6,
0 ° of light polarizing film；7, cmos imaging sensor；8, slide；9, copper mesh；10, purple light glue；11, slot cover；12, slot bottom.
Specific embodiment
Attached drawing of the present invention only for illustration, is not considered as limiting the invention.It is following in order to more preferably illustrate
Embodiment, the certain components of attached drawing have omission, zoom in or out, and do not represent the size of actual product；For art technology
For personnel, the omitting of some known structures and their instructions in the attached drawings are understandable.
Embodiment
As shown in Figure 1 and Figure 2, a kind of phosphatidase micro- based on no lens detects polarisation analyzer, including from the bottom up according to
The secondary light source 1 being arranged, the micro-flow groove 5 containing liquid crystalline sample and the cmos imaging sensor 7 for image recording, it is described micro-
Chute 5 includes slot cover 11, slot bottom 12, is used for loading liquid positioned at the slide 8 of miniflow trench bottom being anchored and on slide 8
Brilliant copper mesh 9, slot cover 11 and slot bottom 12 are adsorbed with 90 ° of light polarizing film 4 and 0 ° light polarizing films 6 respectively.Specifically, in the present embodiment, institute
Slot cover 11 is stated equipped with micro-flow groove water inlet 2 and micro-flow groove water outlet 3；The slide 8 is fixed on slot bottom 12 by ultraviolet glue 10
Center, the copper mesh 9 is fixed on the center of slide 8 by ultraviolet glue 10；The light source 1 is for peak wavelength
450nm, the monochromatic LED lamp that power is 0.5W；90 ° of light polarizing films, 4,0 ° of light polarizing films 6 with a thickness of 0.1~0.5mm；The CMOS at
As sensor 7 pixel size be 2.2 μm, pixel be 5,000,000 (1944 × 2592), imaging sensing area be 24.4mm2。
Further, in this embodiment the distance between the light source 1 and the slot cover 11 of micro-flow groove 5 are 10~13cm.When
When light source 1 is too small at a distance from micro-flow groove 5, the light source optical power that cmos imaging sensor 7 receives is stronger, be easy to cause image
Overexposure.The distance between slot bottom 12 and cmos imaging sensor 7 of the micro-flow groove 5 are 0.5~3mm.It is clear to influence shooting image
Clear the main reason for spending is micro-flow groove apart from the distance between cmos imaging sensor, and distance between the two is smaller, cmos imaging
Sensor it is better at the clarity of image, it is contemplated that the limitation of system itself, the present invention in micro-flow groove slot bottom 12 arrive CMOS
The distance of sensor 7 is about 0.5~3mm best.
Using the method for the phosphatidase polarisation analyzer detection phosphatidase, include the following steps:
S11. the slide 8 of one piece of 1cm × 1cm after cleaning is placed in glass dish, and pours into food into glass dish
Slide is impregnated 25~35min until slide 8 is submerged by mermaid etching liquid, i.e. progress 8 hydrophilic treated of slide；
S12. the slide 8 after impregnating is rinsed with deionized water；
S13. the flushed slide 8 of deionized water is placed into the DMOAP aqueous solution that volumetric concentration is 0.5%~1%
5~15min is impregnated, then is cleaned with deionized water, is then placed into and is heated to 75~85 DEG C of slides being anchored 8 in baking oven,
Carry out the anchoring of slide 8；
S14., the slide 8 being anchored is placed in the slot bottom 12 of micro-flow groove with tweezers, then drips upper 1 in the diagonal angle of slide 8
~2 drop ultraviolet glues, use ultraviolet radiation-curable ultraviolet glue 10；
S15. 285 μm of 75 mesh aperture copper mesh is placed in the surface of glass slide in micro-flow groove, and in 1 drop purple light of surface of glass slide drop
Glue is shifted copper mesh and the rigid accessible position of purple light glue 10, fixed copper mesh into tweezers；
S16. solidify ultraviolet glue using ultraviolet light, by 1~2 uniform coating of drop nematic liquid crystal 4- cyano -4'- amyl connection
Into copper mesh, extra liquid crystal is drawn completely with capillary, covers micro-flow groove slot cover；
S17. the slot bottom of micro-flow groove and slot cover are sealed with purple light glue；
S2, micro-flow groove is placed in above cmos imaging sensor at 0.5~3mm, is successively buffered TBS using small needle tube
Liquid, phosphatide, phosphatidase inject micro-flow groove from micro-flow groove water inlet, observe the brightness change of liquid crystal in visual field.
Fig. 3 (a) be copper mesh on coated with liquid crystal when the case where, Fig. 3 (b) be added TBS buffer as a result, Fig. 3 (c) for
In the case of Fig. 3 (b) be added concentration be 1mg/ml phosphatide about 6 minutes after as a result, concentration is added in the case of being Fig. 3 (c) in Fig. 3 (d)
For the result after 1mg/ml phosphatidase about 3 minutes；It is 1mg/ml phosphatidase about 30 that concentration, which is added, in the case of being Fig. 3 (c) in Fig. 3 (e)
Result after minute.From Fig. 3 (a) it is found that when coated with liquid crystal on copper mesh, visual field is dark state；From Fig. 3 (b) it is found that when drop
After having added TBS buffer, the liquid crystal in visual field brightens, this is because buffer is induction of liquid crystal in horizontal alignment；Such as Fig. 3 (c)
It is found that liquid crystal in visual field is again dimmed after adsorbing phosphatide on nematic liquid crystal film, this is because phosphatide can induce the liquid crystal to be in
It is vertically oriented；Such as Fig. 3 (d), Fig. 3 (e) it is found that after phosphatide enzyme solutions have been added dropwise, the liquid crystal in visual field brightens, this is because phosphorus
Lipase hydrolysis phosphatide, and induce liquid crystal in horizontal alignment.
For the accuracy of verifying present invention shooting phenomenon, using 5x Zeiss microscope respectively to having added TBS buffer, phosphatide
After phosphatide enzyme solutions, the brightness change of liquid crystal is recorded in visual field, as a result as shown in Figure 4, wherein Fig. 4 (a) is on copper mesh
The case where when coated with liquid crystal, Fig. 4 (b) are addition TBS buffer as a result, Fig. 4 (c) is that concentration is added in Fig. 4 (b) to be
It is after 1mg/ml phosphatide about 6 minutes as a result, Fig. 4 (d) is added after concentration is 1mg/ml phosphatidase about 3 minutes in the case of being Fig. 4 (c)
Result；Concentration is added in Fig. 4 (e) in the case of being Fig. 4 (c) be the result after 1mg/ml phosphatidase about 30 minutes.Comparison diagram 3 and figure
4 can correctly record addition TBS it is found that the phosphatidase micro- based on no lens of the present invention detects polarisation analyzer,
The variation of liquid crystal brightness after phosphatide and phosphatide enzyme solutions, compared with traditional petrographic microscope, the present invention also has big visual field,
It is easy to operate, it is small in size, it is portable, it can be realized the advantages such as real-time, quick imaging.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate technical solution of the present invention example, and
It is not the restriction to a specific embodiment of the invention.It is all made within the spirit and principle of claims of the present invention
Any modifications, equivalent replacements, and improvements etc., should all be included in the scope of protection of the claims of the present invention.
Claims (10)
1. a kind of phosphatidase micro- based on no lens detects polarisation analyzer, which is characterized in that including successively arranging from the bottom up
Arrange light source, the micro-flow groove containing liquid crystalline sample and the cmos imaging sensor for image recording of setting, the micro-flow groove packet
Include slot cover, slot bottom, positioned at the slide of miniflow trench bottom being anchored and on slide for loading the copper mesh of liquid crystal, slot cover
It is adsorbed with 90 ° of light polarizing films and 0 ° of light polarizing film respectively with slot bottom.
2. the phosphatidase micro- based on no lens according to claim 1 detects polarisation analyzer, which is characterized in that described
Slot cover is equipped with micro-flow groove water inlet and micro-flow groove water outlet.
3. the phosphatidase micro- based on no lens according to claim 1 detects polarisation analyzer, which is characterized in that described
Light source is the monochromatic LED lamp that peak wavelength is 450nm, power is 0.5W.
4. the phosphatidase micro- based on no lens according to claim 1 detects polarisation analyzer, which is characterized in that described
90 ° of light polarizing films, 0 ° of light polarizing film thickness be 0.1~0.5mm.
5. the phosphatidase micro- based on no lens according to claim 1 detects polarisation analyzer, which is characterized in that described
The pixel size of cmos imaging sensor is 2.2 μm, and pixel is 5,000,000, and imaging sensing area is 24.4mm2。
6. the phosphatidase micro- based on no lens according to claim 1 detects polarisation analyzer, which is characterized in that described
The distance between slot cover of light source and micro-flow groove is 10~13cm.
7. the phosphatidase micro- based on no lens according to claim 1 detects polarisation analyzer, which is characterized in that described
The distance between slot bottom and cmos imaging sensor of micro-flow groove are 0.5~3mm.
8. special using the method for the detection phosphatidase of phosphatidase polarisation analyzer described in claim 1 to 7 any claim
Sign is, includes the following steps:
S1, it slide is subjected to anchoring is placed in the slot bottom of micro-flow groove, then fix a copper mesh on slide, and coat on copper mesh
Liquid crystal, then the slot bottom of micro-flow groove and slot cover are sealed；
S2, micro-flow groove is placed in above cmos imaging sensor at 0.5~3mm, using small needle tube successively by TBS buffer, phosphorus
Rouge, phosphatidase inject micro-flow groove from micro-flow groove water inlet, observe the brightness change of liquid crystal in visual field, that is, detect containing for phosphatidase
Amount.
9. according to the method described in claim 8, it is characterized in that, the process that slide is anchored are as follows:
S11. the slide of one piece of 1cm × 1cm after cleaning is placed in glass dish, and pours into Piranha quarter into glass dish
Liquid is lost, until slide is submerged, slide is impregnated into 25~35min；
S12. the slide after impregnating is rinsed with deionized water；
S13. the flushed slide of deionized water is placed into the DMOAP aqueous solution that volumetric concentration is 0.5%~1% and impregnates 5
~15min, then cleaned with deionized water, it is then placed into baking oven and is heated to 75~85 DEG C, the slide being anchored.
10. according to the method described in claim 8, it is characterized in that, step S1 are as follows:
S14., the slide being anchored is placed in the slot bottom of micro-flow groove with tweezers, it is then ultraviolet in upper 1~2 drop of the diagonal angle drop of slide
Glue uses ultraviolet radiation-curable ultraviolet glue；
S15. copper mesh is placed in the surface of glass slide in micro-flow groove, and in 1 drop purple light glue of surface of glass slide drop, is shifted onto copper mesh with tweezers
With the just accessible position of purple light glue；
S16. solidify ultraviolet glue using ultraviolet light, 1~2 dropping liquid crystalline substance is uniformly coated in copper mesh, it will be extra with capillary
Liquid crystal is drawn completely, and micro-flow groove slot cover is covered；
S17. the slot bottom of micro-flow groove and slot cover are sealed with purple light glue.
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