CN108303123A - A kind of optical fiber sensor head and preparation method and its phosphatidase fibre optical sensor - Google Patents
A kind of optical fiber sensor head and preparation method and its phosphatidase fibre optical sensor Download PDFInfo
- Publication number
- CN108303123A CN108303123A CN201810045758.5A CN201810045758A CN108303123A CN 108303123 A CN108303123 A CN 108303123A CN 201810045758 A CN201810045758 A CN 201810045758A CN 108303123 A CN108303123 A CN 108303123A
- Authority
- CN
- China
- Prior art keywords
- liquid crystal
- fiber
- optical fiber
- rubbing down
- polished
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000835 fiber Substances 0.000 title claims abstract description 124
- 239000013307 optical fiber Substances 0.000 title claims abstract description 88
- 230000003287 optical effect Effects 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 66
- 239000004988 Nematic liquid crystal Substances 0.000 claims abstract description 37
- 239000011521 glass Substances 0.000 claims abstract description 24
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 238000004873 anchoring Methods 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 25
- 239000000243 solution Substances 0.000 claims description 19
- WSFMFXQNYPNYGG-UHFFFAOYSA-M dimethyl-octadecyl-(3-trimethoxysilylpropyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCC[Si](OC)(OC)OC WSFMFXQNYPNYGG-UHFFFAOYSA-M 0.000 claims description 11
- 238000005498 polishing Methods 0.000 claims description 11
- 230000010287 polarization Effects 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 8
- 239000011248 coating agent Substances 0.000 claims description 8
- 238000000576 coating method Methods 0.000 claims description 8
- 239000003292 glue Substances 0.000 claims description 8
- 230000003746 surface roughness Effects 0.000 claims description 8
- 241000252506 Characiformes Species 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000005538 encapsulation Methods 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 230000000640 hydroxylating effect Effects 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims 3
- 238000001514 detection method Methods 0.000 abstract description 18
- 230000008859 change Effects 0.000 abstract description 15
- 239000000463 material Substances 0.000 abstract description 6
- 230000007547 defect Effects 0.000 abstract description 4
- 238000011065 in-situ storage Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 150000003904 phospholipids Chemical class 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- 229910001651 emery Inorganic materials 0.000 description 4
- 239000004094 surface-active agent Substances 0.000 description 4
- 102100026918 Phospholipase A2 Human genes 0.000 description 3
- 101710096328 Phospholipase A2 Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 125000001931 aliphatic group Chemical group 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000013139 quantization Methods 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- -1 but If too big Substances 0.000 description 2
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical group C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- PZJJKWKADRNWSW-UHFFFAOYSA-N trimethoxysilicon Chemical compound CO[Si](OC)OC PZJJKWKADRNWSW-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D5/00—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable
- G01D5/26—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light
- G01D5/268—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light using optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present invention relates to sensor fields, more particularly, to a kind of optical fiber sensor head and preparation method and its phosphatidase fibre optical sensor.Optical fiber sensor head includes the side-polished fiber by anchoring treatment, and side-polished fiber encapsulates on the glass substrate, and upward, the rubbing down area of side-polished fiber is coated with nematic liquid crystal film, and phosphatide is adsorbed on nematic liquid crystal film on rubbing down area surface.The application is coated to liquid crystal as sensitive material on SPF, constitutes the fiber optic sensing device amplified based on liquid crystal optics, and for the detection of biomolecule phosphatidase, can make up traditional defect that phosphatidase sensing is realized using nematic liquid crystal change in orientation.
Description
Technical field
The present invention relates to sensor fields, more particularly, to a kind of optical fiber sensor head and preparation method and its phosphatide
Enzyme fibre optical sensor.
Background technology
The existing method for measuring phosphatidase has titration, colorimetric method, Radio labeled method and fluorescence analysis etc..Titration
Fairly simple, equipment is also cheap, and the aliphatic acid generated by titrating hydrolytic phosphatide makes pH value in solution keep constant, phosphorus
Lipase is weighed by the alkali consumed in titration process.Radio labeled method and fluorescence method need to carry out chemical labeling, behaviour to phosphatide
Make process complexity.
Liquid crystal is found in organism earliest, is gained the name because of the optical characteristics of mobility and crystal with liquid.Liquid crystal
It has been widely used in display field.In addition to this, liquid crystal is also used for the research of sensory field.1998, the U.S.
Abbort research groups realize the detection of biomolecule using the change in orientation and micro-imaging technique of nematic liquid crystal, and will
Achievement in research is published on Science magazines.Biomolecule need not be marked in this technology, cause the extensive of scholar
Concern has started the research boom that biomolecule detection is carried out using liquid crystal.2003, Abbort groups reported a kind of new
The method for measuring phosphatidase, this method are adsorbed on the phosphatide of liquid crystal surfactant, caused liquid crystal aligning hair using phosphatide enzyme hydrolysis
Changing, by petrographic microscope it can be seen that the field luminance of liquid crystal changes, to realize the detection of phosphatidase.This side
Method can monitor distribution of the phosphatide on liquid crystal, understand the process of phosphatide enzyme hydrolysis phosphide, avoid and set using complicated instrument
It is standby.Realize that the report of biomolecule sensing all uses petrographic microscope as detection means using nematic liquid crystal change in orientation,
The advantages of this technology is that biomolecule to be detected need not be marked, and testing result is intuitive, and to the place of sample
Reason is also fairly simple.But petrographic microscope it is bulky, it is expensive, be difficult to carry and can not directly give the inspection of quantization
It surveys as a result, also cannot achieve the on-line checking in situ of biomolecule.
Invention content
The present invention is at least one defect overcome described in the above-mentioned prior art（It is insufficient）, one kind is provided and can be used in reality
When, online, in situ detection phosphatidase optical fiber sensor head, a kind of preparation method of the optical fiber sensor head is also provided and is based on the light
The phosphatidase fibre optical sensor of fine sensing head.
In order to solve the above technical problems, technical scheme is as follows：
A kind of optical fiber sensor head, includes the side-polished fiber Jing Guo anchoring treatment, and side-polished fiber is encapsulated in substrate of glass
On, upward, the rubbing down area of side-polished fiber is coated with nematic liquid crystal film on rubbing down area surface, on nematic liquid crystal film
It is adsorbed with phosphatide.
The purpose that side-polished fiber first passes through anchoring treatment in advance be in order to ensure liquid crystal is in optical fibre interface vertical orientation,
Phosphatide is adsorbed on nematic liquid crystal film, phosphatide can induce the perpendicular orientation of liquid crystal.Side-polished fiber（side-polished
Fiber, SPF）It is very sensitive to the ambient refractive index in rubbing down area.In addition to this, there are one flat regions by SPF, can just use
To place sensitive material.Therefore, the application is coated to liquid crystal as sensitive material on SPF, is constituted and is amplified based on liquid crystal optics
Fiber optic sensing device can make up and traditional utilize nematic liquid crystal change in orientation and for the detection of biomolecule phosphatidase
Realize the defect of phosphatidase sensing.
In said program, nematic liquid crystal is 4- cyano -4'- pentylbiphenyls.Liquid crystal 4- cyano -4'- pentylbiphenyls（5CB）
Refractive index be placed exactly in the sensitive range of rubbing down optical fiber, can be used for make sensor.
In said program, the rubbing down area surface of side-polished fiber is 0 μm -1.5 μm with a distance from fibre core surface.It throws side
Fine rubbing down area surface is polished with a distance from fibre core surface if it is less than 0 μm, fibre core will be ground to, the loss of optical fiber is too big, such as
Fruit is more than 1.5 μm, and sensitivity can substantially reduce.Therefore the distance range is arranged in the present invention, it can be ensured that most of energy of light field
It will be transmitted along the surface of side-polished fiber in the form of evanescent waves, side-polished fiber has very strong evanescent field, this disappears
The field that dies is related with ambient refractive index.
In said program, the rubbing down length of side-polished fiber is 5-20mm.Rubbing down length can make if it is less than 5mm
Difficulty is made, but if being more than 20mm, then fibre loss can be caused too big, therefore it is the most appropriate that 5-20mm is arranged.
In said program, the rubbing down area surface roughness of side-polished fiber is 1.8-2.2 μm.Rubbing down area surface roughness
Setting mainly consider the attachment of liquid crystal, if roughness is too small, liquid crystal is easy to flow on optical fiber, but if too big, system
Optical fiber is easily broken off during making side-polished fiber, therefore balances the attachment of the making and liquid crystal of optical fiber, and the present invention will be thrown
Area's surface roughness is ground to be arranged at 1.8-2.2 μm.
A kind of preparation method of optical fiber sensor head, the method includes：
S1. the side-polished fiber cleaned up is placed into Piranha solution and impregnates certain time, remove Side polishing fiber light
Organic impurities on fibre and so that optical fiber surface hydroxylating；
S2. after the completion of impregnating side-polished fiber is rinsed with deionized water；
S3. hydroxylated side-polished fiber is placed into 0.5%-1%DMOAP aqueous solutions and is impregnated after a certain period of time, spend from
Sub- water cleaning, and be placed into baking oven dry；
S4. it will pass through the processed side-polished fiber encapsulation of DMOAP on the glass substrate；
S5. nematic liquid crystal is coated in the rubbing down area of side-polished fiber, in coating procedure, by side-polished fiber one end
It is connected on laser light source by polarizing optical controller, the other end is connected on light power meter, and real-time online detects liquid crystal coating
The variation of the transmitting optical power of side-polished fiber in the process；
S6. after liquid crystal stabilization, the side-polished fiber for being coated with nematic liquid crystal is placed into buffer solution, then by phosphatide
It is added in buffer solution, phosphatide can be automatically appended to nematic liquid crystal surface, and induce the perpendicular orientation of nematic liquid crystal molecule.
In said program, the specific steps of step S1 include：
S11. side-polished fiber is placed into glass dish, only the part fiber in the area containing rubbing down is put into glass dish, remaining
Then Piranha solution is poured into glass dish outside glass dish, until flooding rubbing down area, is placed into thermal station by part
On, 80 DEG C are heated to, certain time.
In said program, the specific steps of step S4 include：
S41. side-polished fiber is placed on the glass slide cleaned up, is distinguished at the rubbing down area both ends of side wall rubbing down optical fiber
Ultraviolet glue in drop；
S42. side-polished fiber is placed under microscope, upward by rubbing down area surface, ultraviolet radiation-curable ultraviolet glue.
In said program, the nematic liquid crystal is 4- cyano -4'- pentylbiphenyls.
A kind of phosphatidase fibre optical sensor based on nematic liquid crystal optical amplifier, including Fibre Optical Sensor described above
Head, optical fiber sensor head both ends are separately connected the output end of polarization optical controller and the input terminal of light power meter, polarize optical controller
Input terminal connect laser light source, the output end of light power meter connects computer.
The optical fiber sensor head of the present invention is connected with light source, light power meter respectively, the optical fiber with specificity is constituted and passes
Sensor, the sensor are that the change in orientation of nematic liquid crystal caused by bio-molecular interaction is changed into Side polishing fiber light
The real-time online in situ detection of biomolecule is realized in the variation of transmission light field in fibre, so as to complete activity of phospholipase
Line detects.Biomolecule need not be marked in detection process, simple and convenient, need not also bulky, price be used to hold high
It is expensive, carry difficult petrographic microscope, and record using light power meter the change of the transmitting optical power of side-polished fiber in real time
Change the quantization detection that phosphatidase may be implemented.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of optical fiber sensor head of the present invention.
Fig. 2 is the enlarged diagram of side-polished fiber in the present invention.
Fig. 3 is the flow chart of an optical fiber sensor head preparation method in the present invention.
Fig. 4 is a kind of structural representation of the phosphatidase fibre optical sensor based on nematic liquid crystal optical amplifier in the present invention
Figure.
Wherein, 1 is laser light source, and 2 be polarization optical controller, and 3 be closed container, and 4 be light power meter, and 5 be computer, and 6 are
Optical fiber sensor head, 7 be side-polished fiber, and 8 be liquid crystal film, and 9 be substrate of glass, and 10 be ultraviolet glue, and 11 be fibre core, and 12 be packet
Layer, 13 be optical fiber.
Specific implementation mode
The attached figures are only used for illustrative purposes and cannot be understood as limitating the patent；
In order to more preferably illustrate that the present embodiment, the certain components of attached drawing have omission, zoom in or out, the ruler of actual product is not represented
It is very little.
In the description of the present invention, it is to be understood that, in addition, term " first ", " second " are used for description purposes only, and
It should not be understood as indicating or imply relative importance or imply the quantity of indicated technical characteristic." first " that limits as a result,
One or more this feature can be expressed or be implicitly included to the feature of " second ".In the description of the present invention, unless separately
It is described, the meaning of " plurality " is two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " connects
Connect " it shall be understood in a broad sense, for example, it may be being fixedly connected, it may be a detachable connection, or be integrally connected；It can be machine
Tool connects, and can also be electrical connection；It can be directly connected, can also be to be indirectly connected with by intermediary, it may be said that two
Connection inside element.For the ordinary skill in the art, above-mentioned term can be understood in the present invention with concrete condition
Concrete meaning.
The following further describes the technical solution of the present invention with reference to the accompanying drawings and examples.
Embodiment 1
As illustrated in fig. 1 and 2, it is a kind of structural schematic diagram of optical fiber sensor head specific embodiment of the present invention.Referring to Fig. 1 and 2, this tool
A kind of optical fiber sensor head of body embodiment specifically includes the side-polished fiber 7 by anchoring treatment, and side-polished fiber 7 is encapsulated in
In substrate of glass 9, upward, the rubbing down area of side-polished fiber 7 is coated with nematic liquid crystal film 8, nematic on rubbing down area surface
It is adsorbed with phosphatide on phase liquid crystal film 8.
Optical fiber sensor head is made of side-polished fiber 7, and production method is to be put after a single-mode fiber is removed coat
On the emery wheel for setting wheeled optical fiber polishing and burnishing machine, the emery wheel that computer controls wheeled polishing and burnishing machine rotates clockwise, counterclockwise, by optical fiber
The gradual rubbing down of covering falls, and by the insertion loss during real-time online detection fiber rubbing down, makes the rubbing down light of required depth
It is fine.
In this embodiment, the rubbing down length of made side-polished fiber 7 is 5-20mm, preferably 10mm, and rubbing down is long
Degree can to make difficulty, but if being more than 20mm, then fibre loss can be caused too big, therefore 5- is arranged if it is less than 5mm
20mm is the most appropriate.
Rubbing down area surface is 0 μm -1.5 μm with a distance from fibre core surface, preferably 1 μm, the rubbing down area of side-polished fiber
Surface, if it is less than 0 μm, will be ground to fibre core with a distance from fibre core surface, and the loss of optical fiber is too big, if it is greater than 1.5 μm, spirit
Sensitivity can substantially reduce.Therefore the distance range is arranged in the present invention, it can be ensured that most of energy of light field will be with evanescent waves
Form is transmitted along the surface of side-polished fiber, and side-polished fiber 7 has very strong evanescent field, which reflects with environment
Rate is related, and the present embodiment is exactly to utilize the characteristic sensitive to ambient refractive index of side-polished fiber 7.
In this embodiment, the purpose that side-polished fiber 7 first passes through anchoring treatment in advance is in order to ensure liquid crystal is in optical fiber
Interface vertical orientation, adsorbs phosphatide on nematic liquid crystal film 8, and phosphatide can induce the perpendicular orientation of liquid crystal.Side polishing fiber light
Fibre 7（Side-polished fiber, SPF）It is very sensitive to the ambient refractive index in rubbing down area.In addition to this, there are one SPF
Flat region can just be used for placing sensitive material.Therefore, the application is coated to liquid crystal as sensitive material on SPF, is constituted
Based on liquid crystal optics amplification fiber optic sensing device, and for biomolecule phosphatidase detection, can make up traditional utilization to
The defect of phosphatidase sensing is realized in the variation of row phase liquid crystal aligning.
In this embodiment, using nematic liquid crystal as biomolecule sensitive material, preferably nematic liquid crystal uses
4- cyano -4'- pentylbiphenyls（5CB）It realizes.Liquid crystal 4- cyano -4'- pentylbiphenyls（5CB）Refractive index be placed exactly in rubbing down light
In fine sensitive range, it can be used for making sensor.The effective refractive index of nematic liquid crystal 5CB and being arranged with for liquid crystal molecule
It closes, and the arrangement of liquid crystal molecule is influenced by certain biomolecule, the present invention is exactly this feature using liquid crystal molecule
To realize the detection of phosphatidase.
In this embodiment, the rubbing down area surface roughness of side-polished fiber is 1.8-2.2 μm, preferably 2 μm.Rubbing down
The setting of area's surface roughness mainly considers the attachment of liquid crystal, if roughness is too small, liquid crystal is easy to flow on optical fiber, but
If too big, optical fiber is easily broken off during making side-polished fiber, therefore balances the attachment of the making and liquid crystal of optical fiber,
Rubbing down area surface roughness is arranged at 1.8-2.2 μm the present invention.
In the present embodiment, side-polished fiber 7 is encapsulated in substrate of glass 9, and rubbing down area both ends can pass through purple
Outer glue 10 is fixed.
Embodiment 2
The present embodiment is to provide a kind of making optical fiber sensor head described in embodiment 1.
As shown in figure 3, for a kind of flow chart of optical fiber sensor head preparation method specific embodiment of the present invention.Referring to Fig. 3, originally
A kind of specific steps of optical fiber sensor head of specific embodiment include：
S101. the side-polished fiber cleaned up is placed into Piranha solution（70%H2SO4, 30%H2O2）It is middle to impregnate centainly
Time removes the organic impurities on side-polished fiber and makes optical fiber surface hydroxylating；
Wherein, side-polished fiber is to be fallen the side covering rubbing down of single mode optical fiber using wheeled rubbing down method so that in fibre core
Light field leaks constituted optical fiber structure, and specific method is placed into wheeled after a single-mode fiber is removed coat
On the emery wheel of optical fiber polishing and burnishing machine, the emery wheel that computer controls wheeled polishing and burnishing machine rotates clockwise, counterclockwise, and the covering of optical fiber is gradual
Rubbing down falls.When making side-polished fiber, one end of optical fiber is connected with light source, the other end is connected with light power meter,
Insertion loss during real time on-line monitoring optical fiber rubbing down, until rubbing down to required depth.Made Side polishing fiber
The rubbing down length of optical fiber is 5-20mm, and preferably 10mm, rubbing down area surface is 0 μm -1.5 μm with a distance from fibre core surface, preferably
It is 1 μm, about 1 μm from fibre core or so of the rubbing down depth of made rubbing down optical fiber, rubbing down section length about 10mm or so, side
The rubbing down area surface roughness of rubbing down optical fiber is 1.8-2.2 μm, and preferably 2 μm, the made rubbing down optical fiber performed must be with anhydrous
Ethyl alcohol is cleaned up and is dried, it is ensured that free from admixture on optical fiber.
In specific implementation process, step S101 the specific steps are：
Side-polished fiber is placed into glass dish, only the part fiber in the area containing rubbing down is put into glass dish, rest part
Outside glass dish, then the Piranha solution by fresh configuration is poured into glass dish, until flooding rubbing down area, is placed
Onto thermal station, 80 DEG C are heated to, certain time, the time can control within 0.5-1.5 hour, preferably 1 hour, whole
A processing procedure is completed in draught cupboard.
S102. side-polished fiber is rinsed with deionized water after the completion of impregnating, is dried in the air after side-polished fiber is cleaned up
It is dry；
S103., hydroxylated side-polished fiber is placed into the dimethyl stearyl [3- (trimethoxy silicon substrate) of 0.5%-1%
Propyl] ammonium chloride（DMOAP）It impregnates after a certain period of time, such as 10 minutes, is cleaned with a large amount of deionized waters, removal is extra in aqueous solution
DMOAP, be finally placed into baking oven dry, dried 1 hour in the environment of 120 degree.This is to allow DMOAP molecules to fix
To optical fiber rubbing down area surface, DMOAP can occur hydrolysis and autohemagglutination effect, pass through hydrogen bond and optical fiber rubbing down area surface in aqueous solution
Hydroxyl be connected, after heating, hydrogen bond, which can be dehydrated, becomes covalent bond, DMOAP polymer molecules can firmly be connected to optical fiber throwing
The surfaces Mo Qu.DMOAP can also play fixed liquid crystal, prevent other than can be with the perpendicular arrangement of the liquid crystal at induction optical fiber interface
Liquid crystal is detached from the effect of optical fibre interface.
S104. it will pass through the processed side-polished fiber encapsulation of DMOAP on the glass substrate；Specifically, side is thrown
Polishing fibre is placed on the glass slide cleaned up, drips ultraviolet glue respectively at the rubbing down area both ends of side wall rubbing down optical fiber；It avoids
The rubbing down area of optical fiber, is then placed under microscope, and optical fiber is gone to rubbing down surface upward, ultraviolet radiation-curable ultraviolet glue,
Optical fiber encapsulation finishes.
S105. nematic liquid crystal is coated in the rubbing down area of side-polished fiber, in coating procedure, by Side polishing fiber light
Fine one end is connected to by polarizing optical controller on laser light source, and the other end is connected on light power meter, real time on-line monitoring liquid
The variation of the transmitting optical power of side-polished fiber in brilliant coating procedure.After coating liquid crystal, the big appointment of transmitting optical power of optical fiber
Decline 20dB or so, while with polarized light microscope observing optical fiber coating situation, it is ensured that liquid crystal is coated uniformly on optical fiber rubbing down area table
Face.In the present embodiment, nematic liquid crystal is 4- cyano -4'- pentylbiphenyls（5CB）.
S106. after liquid crystal stabilization, the side-polished fiber for being coated with nematic liquid crystal is placed into buffer solution, it is described
Buffer solution is TBS buffer solutions（10 mM Tris、100 mM NaCl、PH8.9）, it is ensured that the activity of biomolecule, then by phosphatide
It is added in buffer solution, allows phospholipid molecule to self-assemble to liquid crystal surfactant, and the liquid crystal at inducing solution interface is gradual by being horizontally oriented
Become vertical orientation.The change of liquid crystal aligning can cause its effective refractive index to change, and transmitting optical power in optical fiber is finally caused to change
Become.It can judge whether phosphatide has self-assembled to liquid crystal surfactant by the situation of change of luminous power in optical fiber in implementation process
And induce the perpendicular orientation of liquid crystal.Phosphatide used in the present embodiment is L-DLPC, this is a kind of synthetic phospholipid phatidylcholine, molecule
Amount is 621.83, and two aliphatic acid tail chains are hydrophobic grouping, and phosphoric acid and choline head group are hydrophilic head.Phosphatide L-DLPC can be certainly
It is dynamic to be attached to liquid crystal surfactant, and induce the perpendicular orientation of nematic liquid crystal molecule.After phosphatide is completely adhered on liquid crystal, have
The optical fiber sensor head of specificity is just made.Polarization optical controller and light power meter can eliminate.
Embodiment 3
On on the basis of embodiment 1, the present invention also provides a kind of, and the phosphatidase optical fiber based on nematic liquid crystal optical amplifier passes
Sensor.As shown in figure 4, the phosphatidase fibre optical sensor includes optical fiber sensor head 6 described in embodiment 1,6 both ends of optical fiber sensor head
It is separately connected the input terminal of the output end and light power meter 4 of polarization optical controller 2, the input terminal connection of polarization optical controller 2 swashs
The output end of radiant 1, light power meter 4 connects computer 5.Wherein, laser light source 1 and the connection, the polarised light that polarize optical controller 2
Controller 2 can be realized with the connection of optical fiber sensor head 6, the connection of optical fiber sensor head 6 and light power meter 4 by optical fiber 13.
In the present invention, Polarization Controller 2 can change the polarization state of input optical fibre sensing head 6 so that certain specific polarization
The light in direction enters optical fiber, and the semiconductor laser of 1310nm may be used in laser light source 1.
The optical fiber sensor head 6 of the present invention is connected with laser light source 1, light power meter 4 respectively, is constituted with specificity
Fibre optical sensor, the sensor are that the change in orientation of nematic liquid crystal caused by bio-molecular interaction is changed into side
The real-time online in situ detection of biomolecule is realized in the variation of transmission light field in rubbing down optical fiber, so as to complete phosphatide enzyme activity
The on-line checking of property.Biomolecule need not be marked in detection process, simple and convenient, also need not use it is bulky,
It is expensive, carry difficult petrographic microscope, and record the transmission light work(of side-polished fiber in real time using light power meter
The quantization detection of phosphatidase may be implemented in the variation of rate.
When specific detection, as shown in figure 4, filling phosphatide enzyme solutions in closed container 3, optical fiber sensor head 6 is placed into phosphorus
When in lipase solution, the phosphatidase hydrolytic phosphatide L-DLPC in solution so that phosphatide is separated from liquid crystal interface, liquid crystal aligning
It changes, liquid crystal effective refractive index is caused to change, the change of refractive index can induce again transmits light in side-polished fiber
It changes by force, this variation can be detected by light power meter, realize the detection of phosphatidase.This sensor may be implemented
Biomolecule need not be marked in phosphatidase in situ detection in solution, simple and convenient.
In example 2, it needs side-polished fiber being dipped into phospholipid solution in the manufacturing process of optical fiber sensor head
To be fabricated to the optical fiber sensor head with specificity, principle therein is：Phosphatide used in embodiment 2 is L-DLPC, this
It is a kind of synthetic phospholipid phatidylcholine, molecular weight 621.83, two aliphatic acid tail chains are hydrophobic grouping, phosphoric acid and choline head base
Group is hydrophilic head.If containing phosphatide in solution, the hydrophilic head of phospholipid molecule can in the solution, and two long hydrophobic tails can ooze
Enter the perpendicular orientation of 5CB liquid crystal in liquid crystal and inducing solution interface.When light field is leaked into from the fibre core of side-polished fiber
When in 5CB liquid crystal, the effective refractive index of 5CB liquid crystal is ne；Phosphatide is adsorbed onto on 5CB liquid crystal and induces liquid crystal molecule is perpendicular to take
During, the effective refractive index of liquid crystal starts to become larger, by (ne+no)/2 become ne（When input optical wavelength is 1310nm,
The n of 5CB liquid crystale=1.6565, no=1.5068）.Side-polished fiber is very sensitive to the effective refractive index of liquid crystal, and research is found
The transmitting optical power of side-polished fiber is begun to ramp up, and amplification is up to 20-30dB.
If the optical fiber sensor head 6 for having adsorbed phosphatide is put into phosphatidase PLA2 solution, PLA2 can hydrolyze attached on liquid crystal
The phosphatide so that the liquid crystal aligning of solution interface turns again to horizontality, and the effective refractive index of 5CB liquid crystal also can be by neBecome
Return (ne+no)/2, the transmitting optical power of side-polished fiber switch to decline, and the range of decrease is up to 20-30dB.Therefore it can pass through side
The variation of rubbing down light transmission fiber power detects PLA2.Compared to traditional liquid crystal biosensor technique, the present invention can be achieved miniature
Sensing head realizes the on-line checking in situ of phosphatidase.
The same or similar label correspond to the same or similar components；
Position relationship described in attached drawing is used to only for illustration, should not be understood as the limitation to this patent；
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be to this hair
The restriction of bright embodiment.For those of ordinary skill in the art, it can also do on the basis of the above description
Go out other various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all in the present invention
Spirit and principle within made by all any modification, equivalent and improvement etc., should be included in the guarantor of the claims in the present invention
Within the scope of shield.
Claims (10)
1. a kind of optical fiber sensor head, which is characterized in that include the side-polished fiber Jing Guo anchoring treatment, side-polished fiber envelope
On the glass substrate, upward, the rubbing down area of side-polished fiber is coated with nematic liquid crystal film, nematic to dress on rubbing down area surface
It is adsorbed with phosphatide on phase liquid crystal film.
2. optical fiber sensor head according to claim 1, which is characterized in that nematic liquid crystal joins for 4- cyano -4'- amyls
Benzene.
3. optical fiber sensor head according to claim 1, which is characterized in that the rubbing down area surface of side-polished fiber is from fibre core
The distance on surface is 0 μm -1.5 μm.
4. optical fiber sensor head according to claim 1, which is characterized in that the rubbing down length of side-polished fiber is 5-
20mm。
5. optical fiber sensor head according to claim 1, which is characterized in that the rubbing down area surface roughness of side-polished fiber
It is 1.8-2.2 μm.
6. a kind of preparation method of optical fiber sensor head, which is characterized in that the method includes：
S1. the side-polished fiber cleaned up is placed into Piranha solution and impregnates certain time, remove Side polishing fiber light
Organic impurities on fibre and so that optical fiber surface hydroxylating；
S2. after the completion of impregnating side-polished fiber is rinsed with deionized water；
S3. hydroxylated side-polished fiber is placed into 0.5%-1%DMOAP aqueous solutions and is impregnated after a certain period of time, spend from
Sub- water cleaning, and be placed into baking oven dry；
S4. it will pass through the processed side-polished fiber encapsulation of DMOAP on the glass substrate；
S5. nematic liquid crystal is coated in the rubbing down area of side-polished fiber, in coating procedure, by side-polished fiber one end
It is connected on laser light source by polarizing optical controller, the other end is connected on light power meter, and real-time online detects liquid crystal coating
The variation of the transmitting optical power of side-polished fiber in the process；
S6. after liquid crystal stabilization, the side-polished fiber for being coated with nematic liquid crystal is placed into buffer solution, then by phosphatide
It is added in buffer solution, phosphatide can be automatically appended to nematic liquid crystal surface, and induce the perpendicular orientation of nematic liquid crystal molecule.
7. preparation method according to claim 6, which is characterized in that the specific steps of step S1 include：
S11. side-polished fiber is placed into glass dish, only the part fiber in the area containing rubbing down is put into glass dish, remaining
Then Piranha solution is poured into glass dish outside glass dish, until flooding rubbing down area, is placed into thermal station by part
On, 80 DEG C are heated to, certain time.
8. preparation method according to claim 6, which is characterized in that the specific steps of step S4 include：
S41. side-polished fiber is placed on the glass slide cleaned up, is distinguished at the rubbing down area both ends of side wall rubbing down optical fiber
Ultraviolet glue in drop；
S42. side-polished fiber is placed under microscope, upward by rubbing down area surface, ultraviolet radiation-curable ultraviolet glue.
9. according to claim 6-8 any one of them preparation methods, which is characterized in that the nematic liquid crystal is 4- cyano-
4'- pentylbiphenyls.
10. a kind of phosphatidase fibre optical sensor based on nematic liquid crystal optical amplifier, which is characterized in that including claim 1-
5 any one of them optical fiber sensor heads, optical fiber sensor head both ends are separately connected the output end and light power meter of polarization optical controller
Input terminal, polarize the input terminal connection laser light source of optical controller, the output end of light power meter connects computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810045758.5A CN108303123B (en) | 2018-01-17 | 2018-01-17 | Optical fiber sensing head, preparation method thereof and phospholipase optical fiber sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810045758.5A CN108303123B (en) | 2018-01-17 | 2018-01-17 | Optical fiber sensing head, preparation method thereof and phospholipase optical fiber sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108303123A true CN108303123A (en) | 2018-07-20 |
| CN108303123B CN108303123B (en) | 2024-02-02 |
Family
ID=62865822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810045758.5A Active CN108303123B (en) | 2018-01-17 | 2018-01-17 | Optical fiber sensing head, preparation method thereof and phospholipase optical fiber sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108303123B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196228A (en) * | 2019-05-09 | 2019-09-03 | 暨南大学 | Based on the micro- phosphatidase detection polarisation analyzer of no lens and detection method |
| CN110196228B (en) * | 2019-05-09 | 2024-10-18 | 暨南大学 | Phospholipase detection polarization analyzer based on lens-free microscopy and detection method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102253010A (en) * | 2011-04-20 | 2011-11-23 | 暨南大学 | Device and method for rapidly detecting swill-cooked dirty oil |
| CN107064063A (en) * | 2016-06-28 | 2017-08-18 | 暨南大学 | A kind of refractive index monitoring device and method based on coring side-polished fiber |
| CN207662427U (en) * | 2018-01-17 | 2018-07-27 | 暨南大学 | A kind of optical fiber sensor head and its phosphatidase fibre optical sensor |
-
2018
- 2018-01-17 CN CN201810045758.5A patent/CN108303123B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102253010A (en) * | 2011-04-20 | 2011-11-23 | 暨南大学 | Device and method for rapidly detecting swill-cooked dirty oil |
| CN107064063A (en) * | 2016-06-28 | 2017-08-18 | 暨南大学 | A kind of refractive index monitoring device and method based on coring side-polished fiber |
| CN207662427U (en) * | 2018-01-17 | 2018-07-27 | 暨南大学 | A kind of optical fiber sensor head and its phosphatidase fibre optical sensor |
Non-Patent Citations (4)
| Title |
|---|
| XINYAN BI, KUN-LIN YANG: "Real-time liquid crystal-based glutaraldehyde sensor", 《SENSORS AND ACTUATORS B》 * |
| 左芳 等: "基于液晶取向改变的化学、生物传感技术的研究进展", 《广州化工》, vol. 39, no. 12, pages 1 - 5 * |
| 李小庆 等: "基于侧边抛磨光纤传感器液晶薄膜光折变特性研究的混合", 《应用光学》, vol. 32, no. 1 * |
| 韩玉琪 等: "用于向列相液晶取向测量的侧边抛磨光纤传感特性研究", 《光学学报》, vol. 34, no. 2, pages 0206006 - 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196228A (en) * | 2019-05-09 | 2019-09-03 | 暨南大学 | Based on the micro- phosphatidase detection polarisation analyzer of no lens and detection method |
| CN110196228B (en) * | 2019-05-09 | 2024-10-18 | 暨南大学 | Phospholipase detection polarization analyzer based on lens-free microscopy and detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108303123B (en) | 2024-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Arjmand et al. | A sensitive tapered-fiber optic biosensor for the label-free detection of organophosphate pesticides | |
| EP2565630B1 (en) | Dye-doped gelatin-coated optical fibers for in situ monitoring of protease activity in wounds | |
| Wong et al. | Polyvinyl alcohol coated photonic crystal optical fiber sensor for humidity measurement | |
| Pathak et al. | Fabrication and characterization of TiO2 coated cone shaped nano-fiber pH sensor | |
| JP5885350B2 (en) | Detection device | |
| Liu et al. | Fiber humidity sensor based on a graphene-coated core-offset Mach–Zehnder interferometer | |
| Tang et al. | Optical fiber bio-sensor for phospholipase using liquid crystal | |
| CN104669104B (en) | Side-polished fiber and preparation method thereof and sensor | |
| JP2007183235A (en) | Surface-plasma resonance measuring apparatus | |
| CN207662427U (en) | A kind of optical fiber sensor head and its phosphatidase fibre optical sensor | |
| CN102939530A (en) | Spr sensor cell and spr sensor | |
| WO2021184675A1 (en) | Fabry-pérot interference-based optical fiber heavy metal ion sensor | |
| Sharma et al. | Fabrication and characterization of pH sensor based on side polished single mode optical fiber | |
| Sakamoto et al. | A novel optical biosensing system using Mach–Zehnder-type optical waveguide for influenza virus detection | |
| Preejith et al. | Total protein measurement using a fiber-optic evanescent wave-based biosensor | |
| CN109342377A (en) | A kind of pH fiber optic sensor system based on fluorescence excitation principle | |
| Huang et al. | In Situ Fluorescence Probing of Molecular Mobility and Chemical Changes during Formation of Dip-Coated Sol− Gel Silica Thin Films | |
| Wu | S-shaped long period fiber grating glucose concentration biosensor based on immobilized glucose oxidase | |
| CN108303123A (en) | A kind of optical fiber sensor head and preparation method and its phosphatidase fibre optical sensor | |
| CN101135637B (en) | Optical fiber dissolved oxygen sensing head and preparation method thereof | |
| US7317847B1 (en) | Optical fibers, devices and methods for making same | |
| CN206420773U (en) | A kind of portable formaldehyde examination device based on fluorescence probe method | |
| Fu et al. | Highly sensitive humidity sensor based on tapered dual side-hole fiber | |
| Huang et al. | Optical fiber Fabry-Perot interferometer refractive index sensor based on Vernier effect for silica colloidal sol aging monitoring | |
| Czarnecka et al. | Magnetically-sensitive nanodiamond thin-films on glass fibers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |