CN110191964A - 确定生物样本中预定来源的游离核酸比例的方法及装置 - Google Patents

确定生物样本中预定来源的游离核酸比例的方法及装置 Download PDF

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CN110191964A
CN110191964A CN201880006995.9A CN201880006995A CN110191964A CN 110191964 A CN110191964 A CN 110191964A CN 201880006995 A CN201880006995 A CN 201880006995A CN 110191964 A CN110191964 A CN 110191964A
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sequencing
nucleic acid
window
predetermined
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CN110191964B (zh
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袁玉英
柴相花
王书元
陈丽娜
周丽君
刘强
张红云
王威
刘娜
尹烨
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

本发明公开了确定生物样本中预定来源的游离核酸比例的方法及装置，包括：(1)对含有游离核酸的生物样本进行核酸测序，以便获得由多个测序数据构成的测序结果；(2)将所述测序结果与参照序列比对，以便确定所述测序结果中落入预定窗口的测序数据的数目；以及(3)基于所述落入预定窗口的测序数据的数目，确定所述生物样本中预定来源的游离核酸比例。

Description

PCT国内申请，说明书已公开。

Claims (56)

  1. PCT国内申请，权利要求书已公开。
CN201880006995.9A 2017-01-24 2018-01-10 确定生物样本中预定来源的游离核酸比例的方法及装置 Active CN110191964B (zh)

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CN2017100552000 2017-01-24
CN201710055200 2017-01-24
PCT/CN2018/072045 WO2018137496A1 (zh) 2017-01-24 2018-01-10 确定生物样本中预定来源的游离核酸比例的方法及装置

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Cited By (2)

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CN114882944A (zh) * 2022-06-22 2022-08-09 珠海碳云智能科技有限公司 基于Metagenome测序的肠道微生物样品宿主性别鉴定方法、装置及应用
CN117106870A (zh) * 2022-12-30 2023-11-24 深圳市真迈生物科技有限公司 胎儿浓度的确定方法及装置

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CN109272046A (zh) * 2018-09-26 2019-01-25 北京科技大学 基于L2重新正则化Adam切换模拟回火SGD的深度学习方法
CN112669282B (zh) * 2020-12-29 2023-02-14 燕山大学 一种基于深度神经网络的脊柱定位方法

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US20150275290A1 (en) * 2012-10-31 2015-10-01 Genesupport Sa Non-invasive method for detecting a fetal chromosomal aneuploidy
WO2016011982A1 (zh) * 2014-07-25 2016-01-28 深圳华大基因股份有限公司 确定生物样本中游离核酸比例的方法、装置及其用途
CN105331606A (zh) * 2014-08-12 2016-02-17 焦少灼 应用于高通量测序的核酸分子定量方法

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CN102329876B (zh) * 2011-10-14 2014-04-02 深圳华大基因科技有限公司 一种测定待检测样本中疾病相关核酸分子的核苷酸序列的方法
NZ717423A (en) * 2012-09-20 2017-08-25 Univ Hong Kong Chinese Non-invasive determination of methylome of fetus or tumor from plasma
WO2015035555A1 (zh) * 2013-09-10 2015-03-19 深圳华大基因科技有限公司 用于确定胎儿是否存在性染色体数目异常的方法、系统和计算机可读介质

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US20150275290A1 (en) * 2012-10-31 2015-10-01 Genesupport Sa Non-invasive method for detecting a fetal chromosomal aneuploidy
WO2016011982A1 (zh) * 2014-07-25 2016-01-28 深圳华大基因股份有限公司 确定生物样本中游离核酸比例的方法、装置及其用途
CN105296606A (zh) * 2014-07-25 2016-02-03 深圳华大基因股份有限公司 确定生物样本中游离核酸比例的方法、装置及其用途
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114882944A (zh) * 2022-06-22 2022-08-09 珠海碳云智能科技有限公司 基于Metagenome测序的肠道微生物样品宿主性别鉴定方法、装置及应用
CN117106870A (zh) * 2022-12-30 2023-11-24 深圳市真迈生物科技有限公司 胎儿浓度的确定方法及装置

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EP3575407B1 (en) 2024-03-27
EP3575407A4 (en) 2020-03-04
WO2018137496A1 (zh) 2018-08-02
CN110191964B (zh) 2023-12-05
EP3575407A1 (en) 2019-12-04

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