CN110187029A - The method for building up and its finger-print of 'Chenxiang Huaqi piece gas-phase fingerprint pattern - Google Patents
The method for building up and its finger-print of 'Chenxiang Huaqi piece gas-phase fingerprint pattern Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Abstract
The invention discloses the method for building up and its finger-print of a kind of 'Chenxiang Huaqi piece gas-phase fingerprint pattern, this method establishes the finger-print of 'Chenxiang Huaqi piece using gas chromatography, is to include the following steps: the preparation of inner mark solution referring to peak with n-octadecane；The preparation of reference substance solution；The preparation of test solution；Take the chromatographic retention of n-octadecane as the relative retention time of the 1 each chromatographic peak being calculated, obtains finger-print, and define the chromatographic condition of the gas phase.The gas-phase fingerprint pattern for the 'Chenxiang Huaqi piece that method provided by the invention is established, establish 11 characteristic peaks in 'Chenxiang Huaqi piece, and it is compared by reference substance and points out wherein 10 peaks with GC-MS method, 20 batches of sample fingerprint similarities are 0.95 or more, the finger-print of foundation avoids the unicity and one-sidedness of 'Chenxiang Huaqi tablet quality control, it ensure that the stability, consistency and controllability of 'Chenxiang Huaqi tablet quality, so that it is guaranteed that safety and validity that 'Chenxiang Huaqi piece uses.
Description
Technical field
The present invention relates to the detection fields of traditional Chinese medicine quality, and in particular to the method for building up of 'Chenxiang Huaqi piece gas-phase fingerprint pattern
And its finger-print.
Background technique
The full side of 'Chenxiang Huaqi piece is made of the 10 taste medicine such as agalloch eaglewood, radix aucklandiae, Pogostemon cablin, rhizoma cyperi, fructus amomi, dried orange peel, has qi-regulating
The effect of soothing the liver, disperse accumulation stomach function regulating, for treating stagnation of liver-QI and stomach-QI, abdominal distention, chest diaphragm feeling of fullness, do not feel like eating, belch pantothenic acid, wherein
It is used as medicine after radix aucklandiae, 4 taste medicinal material distillating extracting oil of Pogostemon cablin, fructus amomi and dried orange peel.Its slightly warm in nature of Pogostemon cablin, acrid flavour, returns spleen,
Stomach, lung channel, Herba Pogostemonis Volatile oil has the function of anti-inflammatory, antibacterial, antibechic and adjusts gastrointestinal tract, and patchouli alcohol is the master of Pogostemon cablin
Want the index components of volatile component and Pogostemon cablin quality evaluation；Its is warm-natured for fructus amomi, acrid flavour, and returns spleen, stomach, kidney channel, fructus amomi are waved
Hair oil has effects that apparent anti-inflammatory analgesic, anti-oxidant, promotion gastrointestinal motility etc. are various, and main component is camphor and second
Sour borneol acetate；Its is warm-natured for dried orange peel, bitter, pungent, return lung, the spleen channel, and Pericarpium Citri Reticulatae volatile oil has good stomach motility enhancing effect.
Compound Chinese medicinal preparation has the characteristics that composition medicinal material is more, constituent is complicated, causes existing Chinese medicine pharmaceutical technology difficult
To ensure tcm product quality stability, traditional Chinese medicine fingerprint can sufficiently extract the chemical component information in Chinese materia medica preparation, lead to
The inherent quality of compound can be reflected to a certain extent by crossing fingerprint spectrum method.At this stage, middle the effective elements of the medicine is most
It is not clear, traditional Chinese medicine quality will be effectively characterized by the way of traditional Chinese medicine fingerprint.'Chenxiang Huaqi piece is recorded in " the Chinese people
Republic's the Sanitation Ministry medicine standard Traditional Chinese medicine historical preparation " the 7th copy, any qualitative, quantitative control for flavour of a drug is not directed in standard
Project processed, 'Chenxiang Huaqi piece Quan Fangzhong volatile component play an important role, for this reason, it is necessary to establish one based on volatile component
The multicomponent and characteristic fingerprint pattern that kind is stablized, precision is high, to science, effectively, comprehensively detection compound Chinese medicinal preparation is sunk
The quality of sweetening treatment gas piece.
Summary of the invention
The present invention provides the method for building up and its finger-print of a kind of 'Chenxiang Huaqi piece gas-phase fingerprint pattern, and this method can be with
Guarantee stability, consistency and the controllability of 'Chenxiang Huaqi tablet quality, so that it is guaranteed that safety that 'Chenxiang Huaqi piece uses and having
Effect property.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of preparation methods of 'Chenxiang Huaqi piece finger-print, include the following steps:
The preparation of inner mark solution；
The preparation of test solution；
The preparation of reference substance solution；
Gas chromatographic detection: it takes the test solution and the reference substance solution to carry out gas chromatographic detection respectively, obtains
Obtain finger-print；
Wherein, the column temperature of gas-chromatography uses temperature programming, and described program heating is 40~60 DEG C of initial temperature, and holding 1~
3min is warming up to 180~250 DEG C with 2.5~5 DEG C/min, keeps 3~10min.
In some specific embodiments of the invention, temperature programming described in the preparation method is 60 DEG C of initial temperature, is protected
It holds 2min and is warming up to 250 DEG C with 2.5 DEG C/min, keep 10min.
In some specific embodiments of the invention, the injector temperature of gas chromatographic column described in the preparation method
250 DEG C, pressure 100kPa；Automatic sampling, 1 μ L of sample volume, split sampling, split ratio 5: 1；Column flow: 1.32mL/min.
In some specific embodiments of the invention, detector described in the preparation method is fid detector, described
250 DEG C of the temperature of detector, H2Flow 40mL/min, air mass flow 400mL/min, N2Flow 30mL/min, total flow
10.9mL/min。
In some specific embodiments of the invention, chromatographic column described in the preparation method is that (fixer is DB-5
5% diphenyl, 95% dimethyl polysiloxane) capillary column, 30m × 0.25mm × 0.25 μm, carrier gas is nitrogen.
In some specific embodiments of the invention, the preparation method of inner mark solution described in the preparation method is to take
N-octadecane is mixed with methanol, obtains inner mark solution.
In some specific embodiments of the invention, the preparation method of test solution described in the preparation method is
'Chenxiang Huaqi piece is taken, ethyl alcohol is added, ultrasonic extraction is supplied weightlessness with ethyl alcohol, shaken up, and stands, and filtrate is crossed 0.22 μm of micropore by filtration
Filter membrane, subsequent filtrate add inner mark solution, then plus ethyl alcohol to scale, shake up, obtain test solution.
In some specific embodiments of the invention, the preparation method of reference substance solution is to take D- in the preparation method
Limonene, Bronyl acetate, patchouli alcohol are mixed with methanol, obtain reference substance solution.
The invention discloses the 'Chenxiang Huaqi piece gas-phase fingerprint pattern established using the above method, have in the finger-print
11 shared peaks, and wherein 10 peaks have been pointed out by GC-MS method.
Compared with prior art, the invention has the following beneficial technical effects:
The method for building up of 'Chenxiang Huaqi piece gas-phase fingerprint pattern disclosed by the invention is used using n-octadecane as reference
Gas chromatography measures its finger-print, has been determined 11 shared peaks in finger-print, and has pointed out wherein 10 by GC-MS method
A peak.20 batches of 'Chenxiang Huaqi piece gas-phase fingerprint pattern similarities 0.95 or more, can effectively characterize its quality, be conducive to
The quality of overall monitor product avoids the unicity and one-sidedness of the control of 'Chenxiang Huaqi tablet quality, guarantees 'Chenxiang Huaqi piece matter
Stability, consistency and the controllability of amount, so that it is guaranteed that safety and validity that 'Chenxiang Huaqi piece uses.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described.
Fig. 1 shows sample chromatogram figure under chromatographic condition 1；
Fig. 2 shows sample chromatogram figure under chromatographic condition 2；
Fig. 3 shows sample chromatogram figure under chromatographic condition 3；
Fig. 4 shows sample chromatogram figure under chromatographic condition 4；
Fig. 5 shows that EtOH Sonicate extracts the gas chromatogram of sample；
Fig. 6 shows the gas chromatogram of methanol ultrasonic extraction sample；
Fig. 7 shows the chromatogram of 'Chenxiang Huaqi piece finger-print and 3 mixing reference substances；
Fig. 8 shows 'Chenxiang Huaqi piece reference fingerprint；
Fig. 9 shows the stacking chart of 20 batches of 'Chenxiang Huaqi piece gas chromatograms；
Figure 10 shows the GC-MS total ion current figure of 'Chenxiang Huaqi piece；
Specific embodiment
Below with reference to embodiment, the present invention is done and is further described in detail, but embodiments of the present invention are not limited to
In this.Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Finger-print provided by the invention the preparation method is as follows:
2.1 chromatographic condition
Chromatographic column: DB-5 (fixer is 5% diphenyl, 95% dimethyl polysiloxane) capillary column, 30m × 0.25mm
× 0.25 μm, carrier gas: nitrogen；Injector temperature: 250 DEG C；Pressure 100kPa；Automatic sampling, 1 μ L of sample volume, split sampling, point
Flow ratio 5: 1；Column flow: 1.32mL/min；Fid detector, 250 DEG C of detector temperature；H2Flow 40mL/min, air mass flow
400mL/min, N2Flow 30mL/min, total flow 10.9mL/min.Column temperature takes temperature programming, and 60 DEG C (40~60 of initial temperature
DEG C), keep 2min (2~5min), be warming up to 250 DEG C with 2.5 DEG C/min (2.5~10 DEG C/min), keep 10min (2~
10min)。
The preparation of 2.2 inner mark solutions
It takes n-octadecane appropriate, adds methanol to be made into solution of every 1mL containing 0.03mg, as inner mark solution.
The preparation of 2.3 reference substance solutions
It takes D- limonene, Bronyl acetate, patchouli alcohol reference substance appropriate, adds methanol that every 1mL is respectively prepared and contain
The solution of 0.06154mg, 0.0069mg, 0.0375mg, 0.0533mg, as reference substance solution.
The preparation of 2.4 test solutions
'Chenxiang Huaqi piece 10 are taken, is ground well, accurately weighed 0.5g is placed in stuffed conical flask, and ethyl alcohol 10mL is added, and is claimed
Amount is ultrasonically treated 30min, lets cool, then weighed quality, supply weightlessness with ethyl alcohol, shake up, and stands, and filtrate is crossed 0.22 μm by filtration
Miillpore filter takes subsequent filtrate 1mL to be placed in 5mL measuring bottle, and inner mark solution 0.5mL is added in precision, adds ethyl alcohol to scale, shakes up, make
For test solution.
In the preparation method of 'Chenxiang Huaqi piece finger-print provided by the invention and the finger-print of acquisition it is raw materials used and
Reagent is available on the market.
Instrument: GC2010 type gas chromatograph, fid detector.D- limonene reference substance (purity > 98%, lot number
Z28D8H51809, Shanghai Yuan Ye Biotechnology Co., Ltd), Bronyl acetate reference substance, patchouli alcohol reference substance (purity >
98%, lot number is respectively MUST-18022605, MUST-17073111, Chengdu Man Site Biotechnology Co., Ltd), positive 18
Alkane (purity > 99%, lot number G9814, Shandong West Asia chemistry limited liability company), methanol is chromatographically pure；Ethyl alcohol is that analysis is pure.
'Chenxiang Huaqi piece come from Taiji Group, lot number are as follows: 18030026,18020015,18010007,18020013,
18030029、18030024、18020012、18030019、18030023、17120095、18040031、18020016、
18030022,18030027,17070058,18040033,18010006,18040030,18030021,17060049, successively
Labeled as S1-S20.
The screening of 1 GC conditions of embodiment
Investigate 4 kinds of different chromatographic conditions:
Chromatographic condition 1: temperature program: total flow 11.8mL/min, split ratio 5: 1 40 DEG C of initial temperature, keep 1min, with 5
DEG C/min is warming up to 180 DEG C, 5min is kept, other conditions are the same as 2.1 chromatographic conditions.
Chromatographic condition 2: temperature program: total flow 11.4mL/min, split ratio 5: 1 50 DEG C of initial temperature, keep 3min, with 3
DEG C/min is warming up to 200 DEG C, 3min is kept, other conditions are the same as 2.1 chromatographic conditions.
Chromatographic condition 3: temperature program: total flow 10.9mL/min, split ratio 5: 1 60 DEG C of initial temperature, keep 5min, with 5
DEG C/min is warming up to 250 DEG C, 5min is kept, runs total time 48min, other conditions are the same as 2.1 chromatographic conditions.
Chromatographic condition 4: temperature program: total flow 10.9mL/min, split ratio 5: 1 60 DEG C of initial temperature, keep 2min, with 2.5
DEG C/min is warming up to 250 DEG C, 10min is kept, runs total time 88min, other conditions are the same as 2.1 chromatographic conditions.
It is as shown in Figure 4 to investigate result.As seen from Figure 1, when for chromatographic condition 4, the peak shape of chromatographic peak, separating effect
And appearance number is best, therefore selects chromatographic condition 4 as final chromatographic condition.
The screening of 2 Extraction solvent of embodiment
Investigate 2 kinds of different Extraction solvents:
Taking lot number is 'Chenxiang Huaqi piece 10 of 17060049, and finely ground, accurately weighed about 0.5g is placed in stuffed conical flask
In, ethyl alcohol 10mL is added, weighs, is ultrasonically treated 30min, lets cool, then weighed quality, supply weightlessness with ethyl alcohol, shake up, stands,
Filtration, crosses 0.22 μm of miillpore filter for filtrate, subsequent filtrate 1mL is taken to be placed in 5mL measuring bottle, and inner mark solution 0.5mL is added in precision, adds
Ethyl alcohol shakes up to scale, as test solution, takes it for chromatographic, it is as shown in Figure 5 to test map.
Taking lot number is 'Chenxiang Huaqi piece 10 of 17060049, and finely ground, accurately weighed about 0.5g is placed in stuffed conical flask
In, methanol 10mL is added, weighs, is ultrasonically treated 30min, lets cool, then weighed quality, supply weightlessness with methanol, shake up, stands,
Filtration, crosses 0.22 μm of miillpore filter for filtrate, subsequent filtrate 1mL is taken to be placed in 5mL measuring bottle, and inner mark solution 0.5mL is added in precision, adds
Methanol shakes up to scale, as test solution, takes it for chromatographic, it is as shown in Figure 6 to test map.
Chromatogram after being extracted by comparing ethyl alcohol and methanol, it can be seen that discovery ethyl alcohol is as Extraction solvent to colors
The extraction of spectral peak is preferable, and baseline is also more stable, therefore selects ethyl alcohol as Extraction solvent.
The preparation of 3 inner mark solution of embodiment
It takes n-octadecane appropriate, adds methanol to be made into solution of every 1mL containing 0.03mg, as inner mark solution.
The preparation of 4 reference substance solution of embodiment
It takes 3 kinds of D- limonene, Bronyl acetate, patchouli alcohol reference substances appropriate, is dissolved, be each configured to proper amount of methanol
After stock solution, obtained mixed reference substance solution is remixed.
The preparation of 5 test solution of embodiment
Taking lot number is 'Chenxiang Huaqi piece 10 of 17060049, and finely ground, accurately weighed about 0.5g is placed in stuffed conical flask
In, ethyl alcohol 10mL is added, weighs, is ultrasonically treated 30min, lets cool, then weighed quality, supply weightlessness with ethyl alcohol, shake up, stands,
Filtration, crosses 0.22 μm of miillpore filter for filtrate, subsequent filtrate 1mL is taken to be placed in 5mL measuring bottle, and inner mark solution 0.5mL is added in precision, adds
Ethyl alcohol to scale, shake up to get.
The method that embodiment 6 establishes 'Chenxiang Huaqi piece gas-phase fingerprint pattern
1) prepare inner mark solution (with embodiment 3)；
2) prepare mixed reference substance solution (with embodiment 4)；
3) prepare the test solution of 'Chenxiang Huaqi piece (with embodiment 5)；
4) chromatography is carried out using gas chromatograph, records chromatogram；
5) it is the relative retention time of the 1 each chromatographic peak being calculated with the chromatographic retention of n-octadecane, is referred to
Line map；
The chromatographic condition of the gas chromatograph: using DB-5, (fixer is the poly- silicon oxygen of 5% diphenyl, 95% dimethyl
Alkane) capillary column (30m × 0.25mm × 0.25 μm), carrier gas: nitrogen；Injector temperature: 250 DEG C；Pressure 100kPa；Automatically into
Sample, 1 μ L of sample volume, split sampling, split ratio 5: 1；Column flow: 1.32mL/min；Fid detector, 250 DEG C of detector temperature；
H2Flow 40mL/min, air mass flow 400mL/min, N2Flow 30mL/min, total flow 10.9mL/min.Column temperature takes program
Heating, keeps 2min by 60 DEG C of initial temperature, is warming up to 250 DEG C with 2.5 DEG C/min, keeps 10min.
Specific finger-print is as shown in fig. 7, the map contains 11 chromatographic peaks.Retained with the chromatography at the peak n-octadecane (S)
Time is that the relative retention time of the 1 each chromatographic peak being calculated is as shown in table 1.
The relative retention time at each peak of table 1
The foundation of 7 'Chenxiang Huaqi piece gas-phase fingerprint pattern of embodiment
The 'Chenxiang Huaqi piece for taking 20 batches of different lot numbers respectively, is measured respectively by embodiment 6.By 20 batches of 'Chenxiang Huaqi piece fingerprints
Map is imported in " similarity evaluation " (2012 editions) software and is handled, and compares the generation of map
Method is median method, and time window width is set as 0.1, carries out full peak match to chromatogram with multi-point correcting method, generates
Reference fingerprint, such as Fig. 8 demarcate 11 shared peaks altogether, wherein n-octadecane be referring to peak (S), the 20 batches of 'Chenxiang Huaqi pieces
Map is superimposed as shown in figure 9, carrying out similarity evaluation to 20 batches of 'Chenxiang Huaqi pieces, the results are shown in Table 2,20 batches of 'Chenxiang Huaqi pieces
Finger-print be not less than 0.95.
2 20 batches of 'Chenxiang Huaqi piece Gas Chromatography Fingerprint similarity results of table
8 GC-MS of embodiment points out each chromatographic peak
Using Gas chromatographyMass spectrometry, initial characterization has been carried out to the peak in 'Chenxiang Huaqi piece finger-print, has been sunk
Sweetening treatment gas piece sample total ion current figure is shown in Figure 10.
Compound that may be present in map is retrieved by system deconvolution in computer, and according to NIST system mass spectrum
Picture library retrieval matching, confirms structure.It is finally compared through reference substance, NIST14 library searching and literature search, to wherein 10
Ingredient is pointed out, and is shown in Table 3.
10 shared peaks points out in 3 Gas Chromatography Fingerprint of table
In summary the invention has the following advantages that
1. the 'Chenxiang Huaqi piece gas-phase fingerprint pattern similarity that method provided by the present invention is established is all larger than 0.95, can have
Its quality of the characterization of effect, is conducive to the quality of overall monitor product.
2. the present invention establishes a kind of 'Chenxiang Huaqi piece characteristic fingerprint pattern common pattern, 11 shared peaks have been demarcated, and
It is compared by reference substance and GC-MS method has pointed out wherein 10 peaks, the fingerprint pattern technology content of foundation is high, avoids said preparation
The unicity and one-sidedness of quality control, so that it is guaranteed that the safety and validity of 'Chenxiang Huaqi piece.
The invention discloses the method for building up and its finger-print of 'Chenxiang Huaqi piece gas-phase fingerprint pattern, those skilled in the art
Member can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications
Apparent to those skilled in the art, they are considered as being included in the present invention.Method and application of the invention
Be described by preferred embodiment, related personnel obviously can not depart from the content of present invention, in spirit and scope it is right
Method described herein and application are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Claims (8)
1. including the following steps: the present invention provides a kind of method for building up of 'Chenxiang Huaqi piece gas-phase fingerprint pattern
The preparation of inner mark solution；
The preparation of reference substance solution；
The preparation of test solution；
Gas chromatographic detection: it takes the test sample and the reference substance to carry out gas chromatographic detection respectively, obtains finger-print；
Wherein, the column temperature of gas-chromatography uses temperature programming, and described program heating is 40~60 DEG C of initial temperature, keeps 1~3min, with
2.5~5 DEG C/min is warming up to 180~250 DEG C, keeps 3~10min.
2. preparation method according to claim 1, which is characterized in that described program heating is 60 DEG C of initial temperature, keeps 2min,
250 DEG C are warming up to 2.5 DEG C/min, keeps 10min.
3. preparation method according to claim 1 or 2, which is characterized in that the injector temperature of the gas chromatographic column:
250℃；Pressure 100kPa；Automatic sampling, 1 μ L of sample volume, split sampling, split ratio 5: 1；Column flow: 1.32mL/min.
4. preparation method according to any one of claims 1 to 3, which is characterized in that the detector is fid detector,
250 DEG C of the temperature of the detector, H2Flow 40mL/min, air mass flow 400mL/min, N2Flow 30mL/min, total flow
10.9mL/min。
5. preparation method according to any one of claims 1 to 4, which is characterized in that the chromatographic column is DB-5 capillary
Column, 30m × 0.25mm × 0.25 μm, carrier gas are nitrogen.
6. preparation method according to any one of claims 1 to 5, which is characterized in that the preparation method of the inner mark solution
To take n-octadecane to mix with methanol, inner mark solution is obtained.
7. preparation method according to any one of claims 1 to 6, which is characterized in that the preparation side of the test solution
Method is to take 'Chenxiang Huaqi piece, adds ethyl alcohol, is weighed, and ultrasonic extraction is supplied weightlessness with ethyl alcohol, shaken up, and is stood, filtration, by filtrate mistake
0.22 μm of miillpore filter, subsequent filtrate add inner mark solution, then plus ethyl alcohol to scale, shake up, obtain test solution.
8. preparation method according to any one of claims 1 to 7, which is characterized in that the preparation side of the reference substance solution
Method is that D- limonene, Bronyl acetate, patchouli alcohol is taken to mix with methanol, obtains reference substance solution.
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