CN110187024A - The remaining measuring method of dimethyl sulfoxide in a kind of source of people retinal pigment epithelium injection - Google Patents
The remaining measuring method of dimethyl sulfoxide in a kind of source of people retinal pigment epithelium injection Download PDFInfo
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- CN110187024A CN110187024A CN201910444295.4A CN201910444295A CN110187024A CN 110187024 A CN110187024 A CN 110187024A CN 201910444295 A CN201910444295 A CN 201910444295A CN 110187024 A CN110187024 A CN 110187024A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention discloses the remaining measuring methods of dimethyl sulfoxide in a kind of source of people retinal pigment epithelium injection, comprising: (1) source of people retinal pigment epithelium injection pre-treatment: taking supernatant to be detected after injection centrifugation；(2) it is mixed after taking a certain amount of supernatant that appropriate dilution is added；(3) mixed liquor is subjected to retention analysis with internal standard method for gas chromatography technology, finds optimal chromatographic condition and carries out methodology validation；(4) the remaining measurement of dimethyl sulfoxide in injection is carried out using the method being verified.The present invention provides one kind simply, fast and effective, result is accurate and reliable, and provides practicable method for dimethyl sulfoxide residual quantity in control source of people retinal pigment epithelium injection.
Description
Technical field
The present invention relates to analytical chemistry fields, and in particular to dimethyl is sub- in a kind of source of people retinal pigment cell injection
The method of sulfone residues detecton.
Background technique
Retinal pigment epithelium (RPE) is the continuous cell monolayer outside the retina, because its sieve-like form and
Melanin abundant in cytoplasm and gain the name, they play critical function in the maintenance and self-renewing of retina cell, such as: gulping down
Bite the OS for the photoreceptor cell that digestion falls off；Promote the regeneration of medium 11-cis retinal important in view circulation；It adjusts intraocular
Immune response；Participation forms retina-vascular barrier etc..This physiological function of RPE cell is considered and related eye extremely
The generation of section's disease is closely related, and Best vitelliform macular malnutrition (Best is easily led to if RPE barrier cell dysfunction
Vitelliform macular dystrophy, BVMD) and adult vitelliform macular malnutrition (adult-onset
Vitelliform macular dystrophy, AVMD).Connection or function between RPE basilar memebrane and Bruch ' s membrane
Can be considered extremely age-related property macular degeneration (age-related macular degeneration, AMD) and
The generation of Sorsby ' s fundus dystrophy is related.
AMD is mainly shown as that retinal pigment epithelium declines acromere disk film phagocytosis digestion power, as a result
Make the disk film residual body retention not digested completely in basal part cell magma, and is deposited on Bruch to extracellular exclusion
Film forms glass-film wart.AMD is divided to for stemness and two kinds moist, wherein stemness AMD is more common, it is due to RPE progressive
A kind of disease of choroidal capillaries caused by malnutrition and light receptor missing.Moist AMD has been typically characterised by train of thought
Film new vessels (choroidal neovascularization, CNV) generate, to cause serious visual loss.In addition to
Cell replacement therapy there is no the effective way for reversing this degenerative process and restoring eyesight at present.
The test of RPE cell therapy AMD is shown by transplanting normal RPE cell in subretinal space, can delay to carry out
The loss of visual function of property.Test of the RPE cell transplantation on RPE genetic defect animal model and AMD patient, all display can
Delay the retrogression pathological changes of retina, improves visual performance.RPE cell is more stable in the autotransplantation of subretinal space, and
With long-term curative effect.
Residual solvent in drug means in the production of bulk pharmaceutical chemicals or auxiliary material, and uses in formulation process
, but fail in technical process the organic solvent completely removed.Dimethyl in source of people retinal pigment epithelium injection
Sulfoxide is classified as third class solvent in the guideline of ICH Q3C (R7) residual solvent, and belonging to has genotoxic potential to human body
Solvent.The residual of organic solvent in known drug is measured using a variety of different analysis method realizations in the prior art.
Such as the methods of gas chromatography, liquid chromatography and Mass Spectrometer Method.Document is " in capillary gas chromatography gatifloxacin
Dimethyl sulfoxide residual quantity " (the 10th phase of volume 17 in 2008), it is realized using capillary gas chromatography in gatifloxacin two
The quantitative detection of methyl sulfoxide.(2011 volume 14 of document " GC method measures residual solvent dimethyl sulfoxide in palm piperazine pool piperazine "
12nd phase) quantitative determination that dimethyl sulfoxide in piperazine is moored to palm piperazine is realized using GC method.However above two method is being applied
When into practical biological sample, since dimethyl sulfoxide concentration is low, there are endogenic interference, and provide the sample size of detection compared with
Few, when the above method being caused to be applied in such product, accuracy, precision and sensitivity etc., which will be unable to reach regulation, to be wanted
It asks.Therefore developing one kind has weight to the remaining detection of dimethyl sulfoxide in realization source of people retinal pigment epithelium injection
The meaning wanted.
Summary of the invention
For it is existing in the prior art above-mentioned the problems such as, can be simple and quick, accurate there is an urgent need to research and develop that one kind is able to achieve
The technical side that is tested and analyzed is remained to the dimethyl sulfoxide (DMSO) in source of people retinal pigment epithelium injection
Case.In the prior art not about the remaining measurement of dimethyl sulfoxide in cell class product, there is a small amount of document to register in chemical drug
About the technology of dimethyl sulfoxide residues detecton, capillary gas chromatography is mainly used.The present invention is directed primarily to carefully
Born of the same parents' product, the prior art can not obtain identical/similar detection method, and residual limit is lower in addition, the detection limit of gas-chromatography
It is unable to satisfy its requirement, therefore using the method for internal standard method for gas chromatography, and then grope optimal chromatographic condition and detected.
It remains and carries out the present invention provides the dimethyl sulfoxide in a kind of pair of source of people retinal pigment epithelium injection
The method of detection and analysis, the method use internal standard method for gas chromatography technology and carry out analysis detection, while using organic
Solvent is diluted dissolution to actual sample (source of people retinal pigment epithelium injection), reduces the interference effect of matrix
It answers, significantly improves the sensitivity and accuracy of detection, to meet relevant laws and regulations requirement, that is, meet " Chinese Pharmacopoeia " 2015
The 4th Quality Evaluation of Chinese Medicinal amount standard method of analysis verification guide principle of version.
The present invention provides dimethyl sulfoxide residues detectons in a kind of source of people retinal pigment epithelium injection to analyze
Method, using organic reagent as sample retarder thinner, with what is obtained after the centrifugation of source of people retinal pigment epithelium injection
Centrifuged supernatant is as sample to be tested, using dimethyl sulphoxide control product as external standard, carries out to practical sample to be tested and reference substance
Dilution carries out analysis detection using internal standard method for gas chromatography technology after membrane filtration.
The present invention, which is used, calculates testing result with external standard method.Wherein, external standard method refers to: a certain amount of reference substance being taken to be made pair
According to product solution, carry out sample treatment and detection in parallel with sample to be tested, measure in reference substance solution and testing sample solution to
The peak area for surveying substance, is calculated by formula content:
Content (CX)=AX/AR(wherein AXRefer to the peak area of test sample (sample to be tested), ARIt is the peak area of reference substance,
CXIt is test sample (sample to be tested) concentration)
Wherein, the organic reagent includes one or more of methanol, acetonitrile, ethyl alcohol etc..
The remaining measuring method of dimethyl sulfoxide in source of people retinal pigment epithelium injection of the present invention, including it is following
Step:
(1) preparation of sample to be tested: source of people retinal pigment epithelium injection is transferred in centrifuge tube, centrifugation,
Supernatant after taking centrifugation adds diluent to be diluted up to sample to be tested；
(2) preparation of reference substance solution: weighing dimethyl sulphoxide control product into volumetric flask, adds diluent to carry out gradient dilute
It releases, obtains reference substance solution；
(3) analysis detection: firstly, the reference substance solution that step (2) is obtained through membrane filtration, then utilizes gas-chromatography
It is measured with mass spectrometric hyphenated technique, obtains standard calibration curve；Then sample to be tested step (1) obtained is through membrane filtration
Afterwards, analysis detection is carried out using internal standard method for gas chromatography technology, it is then public by the standard calibration curve and calculating
Formula calculates the residual quantity of dimethyl sulfoxide in sample to be tested；
If continuous mode exists, the concentration of the sample to be tested exceeds the range of linearity of reference substance, then need to product to be tested into
Row dilution.
In step (1), contain inorganic salts in the sample to be tested, including magnesium chloride, calcium chloride, sodium acetate, sodium citrate,
One or more of Deng.
In step (1), the condition of the centrifugation is that 500g-1500g is centrifuged -15 minutes 2 minutes；Preferably, for 702g from
The heart 5 minutes.
In step (1), the purpose of the centrifugation is removal cell and large particulate matter, prevents blocking instrument pipeline.
In step (1), the diluent is one or more of methanol, acetonitrile, ethyl alcohol etc.；Preferably, the dilution
Agent is ethyl alcohol.
In step (1), the diluting condition preferably takes sample about 25mg after centrifugation to add diluent dilute in 5ml measuring bottle
Release simultaneously constant volume.
The purpose that step (1) of the present invention is diluted is the damage for reducing inorganic salts and water to chromatographic column.
In step (2), the reference substance is dimethyl sulphoxide control product；The gradient dilution are as follows:
Reference substance stock solution 1: precision weighs dimethyl sulfoxide 50.00mg and is placed in 10ml volumetric flask, adds diluent constant volume,
Shake up to get.
Reference substance stock solution 2: precision pipettes 0.5ml reference substance stock solution 1 and is placed in 50ml volumetric flask, adds diluent fixed
Hold, shake up to get.
Reference substance stock solution 3: precision pipettes 2.0ml reference substance stock solution 2 and is placed in 20ml volumetric flask, adds diluent fixed
Hold, shake up to get.
Reference substance solution: precision pipettes 1.0ml reference substance stock solution 3 and is placed in 10ml volumetric flask, adds diluent constant volume, shakes
It is even to get.
In step (2), the diluent is one or more of methanol, acetonitrile, ethyl alcohol etc..
In step (3), the filter membrane include one of nylon membrane, polytetrafluoroethylene film, PVDF membrane etc. or
It is several；It preferably, is nylon membrane.
In step (3), the aperture of the filter membrane is 0.22 μm, 0.3 μm, 0.45 μm or 0.65 μm；It preferably, is 0.22 μ
m。
In step (3), the condition that analysis detection is carried out using internal standard method for gas chromatography technology are as follows: gas-chromatography
Condition: 10 VF-WAXms, SUPELCOWAX in capillary chromatographic column, SUPEROX II, CB-WAX, Stabilwax, BP-
20,007-CW, Carbowax, Rtx-wax capillary chromatographic column, sample volume are 0.5 μ l-2 μ l, 160 DEG C -260 of injector temperature
DEG C, flow velocity: 0.5ml/min-1.5ml/min, split ratio (15-25): 1, carrier gas is helium, using temperature programming；Detector is
Mass spectrometer detector: acquisition mode be SIM mode, quota ion: 61/65/78, solvent delay -5 minutes 1 minute, detector close
Time: -20 minutes 6 minutes.
Wherein, the capillary chromatographic column be VF-WAXms, SUPELCOWAX 10, SUPEROX II, CB-WAX,
Stabilwax, BP-20,007-CW, Carbowax, Rtx-wax capillary chromatographic column.
Preferably, use 100% polyethylene glycol for the capillary chromatographic column of stationary phase: (Agilent VF-WAXms, 30m
× 0.25 mm × 0.25 μm), sample volume is 1 μ l, and injector temperature is 200 DEG C, flow velocity: 1.0ml/min, split ratio 20:1 are carried
Gas is helium；Using temperature programming；Detector is mass detector: acquisition mode be SIM mode, quota ion: 61/65/78,
Solvent delay 3min, detector shut-in time: 9 minutes.
Wherein, the condition of described program heating are as follows: 70 DEG C -120 DEG C run -10 minutes 3 minutes, then with 20 DEG C -35 DEG C
170 DEG C -240 DEG C are risen to per minute, are run -5 minutes 1 minute；Then it is run -6 minutes 2 minutes at 200 DEG C -280 DEG C.
Preferably, the condition of described program heating are as follows: 90 DEG C run 5 minutes, rise to 210 DEG C per minute with 25 DEG C, operation
2.2 minute；It is run 2 minutes at 230 DEG C.
In a specific embodiment, the step of carrying out analysis detection using internal standard method for gas chromatography technology is such as
Under:
Use 100% polyethylene glycol for the capillary chromatographic column of stationary phase: (AgilentVF-WAXms, 30m × 0.25mm
× 0.25 μm), injector temperature is 200 DEG C；Temperature program are as follows: 90 DEG C run 5 minutes, rise to 210 DEG C per minute with 25 DEG C,
Operation 2.2 minutes；Flow velocity is 1.0ml/min；It is run 2 minutes at 230 DEG C；Sample volume is 1 μ l；Split ratio is 20:1；Carrier gas is
Helium；Detector is mass detector: acquisition mode is SIM mode；Quota ion: 61/65/78；Solvent delay 3min；Detection
The device shut-in time: 9 minutes.
Reaction mechanism involved in the method for the present invention are as follows: sample to be tested vaporizing chamber vaporization after by inert gas (i.e. carrier gas,
Also be mobile phase) bring chromatographic column into, liquid or solid stationary phase is contained in column, due to the boiling point of each component in sample, polarity or
Absorption property is different, and every kind of component tends to form distribution or adsorption equilibrium between mobile phase and stationary phase.But due to carrying
Gas is mobile phase, is difficult to establish distribution or adsorption equilibrium.Also just because of the flowing of carrier gas, carry out sample component during exercise
Repeated multiple times distribution or adsorption/desorption is attached, so that the big component of concentration first flows out chromatographic column in carrier gas, and in stationary phase
It is flowed out after the big component of distribution concentration.After group distributes chromatographic column, mass detector is immediately entered.Mass detector can incite somebody to action
Sample component is changed into electric signal, and the size of electric signal is directly proportional to the amount of tested component or concentration, final to calculate to test sample
The result of product.
In the detection process, dimethyl sulfoxide (DMSO) content calculation formula is as follows by the present invention:
In formula: WSTDDMSO mass (mg) in=reference substance
WSPLThe quality (mg) of=sample to be tested
BSTDThe average peak area of DMSO in=reference substance
ASPLThe peak area of DMSO in=sample to be tested
VSPL=sample to be tested dilutes volume (ml)
VSTD=reference substance dilutes volume (ml)
PSTDThe content (%) of=reference substance
The invention also provides application of the measuring method in measurement cell class product in dimethyl sulfoxide residual.
Central inventive point of the present invention include the following:
1, the present invention provides one kind for detecting the remaining method of dimethyl sulfoxide in biological agent and Related product, i.e.,
Internal standard method for gas chromatography technology.
2, the invention proposes the optimal detection conditions of internal standard method for gas chromatography technology, can detecte micro two
The residual of methyl sulfoxide, the minimum residual quantity that can detecte the dimethyl sulfoxide containing 0.01ug/ml of the method for the present invention, significantly improves
The sensitivity and accuracy of detection.
The present invention innovates to be overcome in R&D process:
1, damage of the inorganic salts and water contained by sample itself to chromatographic column.
2, sample belongs to micro costly medicine, so the sample size that can provide detection is considerably less.
3, dimethyl sulfoxide residual is very low in sample, and standard is 0.01% (m/m), can not be carried out using gas-chromatography
Accurate quantitative analysis.
In one embodiment, the measuring method specifically includes the following steps:
(1) collection of centrifuged supernatant: source of people retinal pigment epithelium injection is transferred in centrifuge tube,
It is centrifuged -15 minutes 2 minutes under conditions of 500g-1500g, the supernatant after taking centrifugation is to get sample to be tested.
(2) sample to be tested dilutes: (supernatant after centrifugation is with quality by the sample to be tested 25mg-50mg after taking above-mentioned centrifugation
Meter) be placed in 5ml-25ml volumetric flask, add diluent methanol or acetonitrile or ethyl alcohol to dilute constant volume, shake up up to it is diluted to
Sample solution.
(3) preparation of reference substance solution: dimethyl sulphoxide control product 50mg-100mg is weighed into volumetric flask, adds diluent
Methanol/acetonitrile/ethyl alcohol dilutes step by step, obtains the reference substance solution that concentration is 0.5 μ g/ml-2.0 μ g/ml.
(4) it analysis detection: is obtained by diluted testing sample solution that above-mentioned steps (2) obtain and through step (3)
Reference substance solution carries out analysis detection after membrane filtration, using internal standard method for gas chromatography technology.
In measuring method of the present invention, the determination condition in the internal standard method for gas chromatography technology is same as above.
In one embodiment, internal standard method for gas chromatography technology, You Anjie employed in the method for the present invention
Human relations 7890B gas chromatograph is equipped with Agilent 5977B tandem mass spectrometer composition.
Advantages of the present invention and beneficial effect include:
The present invention is diluted sample to be tested using organic reagent, not only avoids in sample water to the damage of capillary column
Wound, while also reducing interference of the sample copy bottom to chromatographic isolation.Because aqueous diluent has damage chromatographic column, can only make
Use organic reagent；The type for selecting organic reagent is mainly to consider the separating effect of object in the chromatography column.Use gas phase color
The analysis method that spectrum is detected with mass spectrometric hyphenated technique, preferred chromatographic condition are as follows: use 100% polyethylene glycol for stationary phase
Capillary chromatographic column: (Agilent VF-WAXms, 30m × 0.25mm × 0.25 μm), sample volume be 1 μ l, injector temperature
200 DEG C, flow velocity: 1.0ml/min, split ratio 20:1, carrier gas are helium.Using temperature programming, condition are as follows: 90 DEG C run 5 minutes,
210 DEG C are risen to per minute with 25 DEG C, are run 2.2 minutes, are then run 2 minutes at 230 DEG C.Detector is mass detector:
Acquisition mode be SIM mode, quota ion 61/65/78, solvent delay 3 minutes, the detector shut-in time 9 minutes.
The method of the present invention has the advantages that simple and quick, highly sensitive and high accuracy, the rate of recovery reach 98%, the method
Repeatability are as follows: the rate of recovery mean value of 6 parts of samples is 103%, and detection is limited to 0.01 μ g/ml, and the range of linearity is 0.0501 μ g/
Ml-1.0018 μ g/ml, precision are that the rate of recovery mean value of 6 parts of samples is 97%, are quantitatively limited to (LOQ) 0.0501 μ g/ml)
To the detection of source of people retinal pigment epithelium injection present preferable applicability, specificity, quantitative limit and detection limit,
Linearity and range, accuracy, stability of solution, Intermediate precision and durability.
Detailed description of the invention
Fig. 1 is blank solution mass spectrogram in embodiment 2-4.
Fig. 2 is 1 Mass Spectrometer Method analysis chart of dimethyl sulphoxide control product in embodiment 2-4.
Fig. 3 is 2 Mass Spectrometer Method analysis chart of dimethyl sulphoxide control product in embodiment 2-4.
Fig. 4 is source of people retinal pigment epithelium injection (lot number 201708093RCY-1) SPL-1 in embodiment 2
Mass Spectrometer Method analysis chart.
Fig. 5 is source of people retinal pigment epithelium injection (lot number 201708093RCY-1) SPL-2 in embodiment 2
Mass Spectrometer Method analysis chart.
Fig. 6 is source of people retinal pigment epithelium injection (lot number 201710097RCY-1) SPL-3 in embodiment 3
Mass Spectrometer Method analysis chart.
Fig. 7 is source of people retinal pigment epithelium injection (lot number 201710097RCY-1) SPL-4 in embodiment 3
Mass Spectrometer Method analysis chart.
Fig. 8 is source of people retinal pigment epithelium injection (lot number 201712121RCY-4) SPL-5 in embodiment 4
Mass Spectrometer Method analysis chart.
Fig. 9 is source of people retinal pigment epithelium injection (lot number 201712121RCY-4) SPL-6 in embodiment 4
Mass Spectrometer Method analysis chart.
Specific embodiment
In conjunction with following specific embodiments and attached drawing, the present invention is described in further detail.Implement process of the invention,
Condition, experimental method etc. are in addition to what is specifically mentioned below the universal knowledege and public common sense of this field, this hair
It is bright that there are no special restrictions to content.Following embodiment is only used for that the present invention is further explained, but should not be construed as to limit of the invention
System.
Accuracy, precision, repeatability of the method for the present invention etc. are confirmed in methodology validation.
The chemical reagent such as diluent used in the present invention such as methanol, ethyl alcohol, acetonitrile are chromatographically pure, and water is ultrapure water.
In measuring method of the present invention, sample treatment liquid utilizes internal standard method for gas chromatography after the filtration of 0.22um nylon membrane
Technology carries out analysis detection.
Embodiment 1
The method validation of residual solvent dimethyl sulfoxide assay in source of people retinal pigment epithelium injection:
Dimethyl sulphoxide control product and source of people retinal pigment epithelium injection (201805029RCY-QC1), injection
Then liquid does solvent preparation with ethyl alcohol using centrifuging and taking supernatant is preceding needed, respectively preparation system applicability, specificity, quantitative limit and
Detect limit, linearity and range, accuracy, repeatability, stability of solution, Intermediate precision and serviceability test sample.It is each to test
For card project according to set standard sample detection, concrete operations are as follows:
(1) reference substance solution (two parts of configured in parallel)
Diluent/blank solution: ethyl alcohol
Reference substance stock solution 1-1: precision weighs 50.19mgDMSO and is placed in 10ml volumetric flask, adds diluent constant volume, shakes
It is even to get.
Reference substance stock solution 1-2: precision weighs 50.21mgDMSO and is placed in 10ml volumetric flask, adds diluent constant volume, shakes
It is even to get.
Reference substance stock solution 2-1: precision pipettes 0.5ml reference substance stock solution 1-1 and is placed in 50ml volumetric flask, adds diluent
Constant volume, shake up to get.
Reference substance stock solution 2-2: precision pipettes 0.5ml reference substance stock solution 1-2 and is placed in 50ml volumetric flask, adds diluent
Constant volume, shake up to get.
Reference substance stock solution 3-1: precision pipettes 2.0ml reference substance stock solution 2-1 and is placed in 20ml volumetric flask, adds diluent
Constant volume, shake up to get.
Reference substance stock solution 3-2: precision pipettes 2.0ml reference substance stock solution 2-2 and is placed in 20ml volumetric flask, adds diluent
Constant volume, shake up to get.
Reference substance solution 1:(system suitability solution 1, STD1): precision pipettes 1.0ml reference substance stock solution 3-1 and is placed in
In 10ml volumetric flask, add diluent constant volume, shake up to get.
Reference substance solution 2:(system suitability solution 2, STD2): precision pipettes 1.0ml reference substance stock solution 3-2 and is placed in
In 10ml volumetric flask, add diluent constant volume, shake up to get.
(2) specificity solution
Diluent/blank solution: ethyl alcohol
Reference substance solution: reference substance solution 1 under " (1) reference substance solution " item is taken.
Sample blank solution: it weighs 24.67mg sample (lot number 201805029RCY-QC1) and is placed in 5ml volumetric flask, add
Diluent dissolution and constant volume, shake up, with 0.22um nylon membrane filter to get.
Sample mark-on solution: weighing 24.82mg sample (lot number 201805029RCY-QC1) and be placed in 5ml volumetric flask, to
Wherein be added 0.5ml reference substance stock solution 3-1, add diluent dissolution and constant volume, shake up, with 0.22um nylon membrane filter to get.
(3) detection limit and quantitative limit solution
Quantitative limit solution: precision pipettes reference substance solution 1 under 1.0ml " (1) reference substance solution " item and is placed in 10ml volumetric flask
In, add dilution dilution agent and constant volume, shake up to get.
Detection limit solution: precision pipettes the above-mentioned quantitative limit solution of 2.0ml (DMSO dilutes dissolved solution with ethyl alcohol) and sets
In 10ml volumetric flask, add dilution dilution agent constant volume, shake up to obtain the final product.
(4) linear solvent
L-200%: precision pipettes reference substance stock solution 3-1 to the 10ml capacity under 2.0ml " (1) reference substance solution " item
Bottle, to dilute dilution agent and constant volume, shake up to get.
L-150%: precision pipettes reference substance stock solution 3-1 to the 10ml capacity under 1.5ml " (1) reference substance solution " item
Bottle, to dilute dilution agent and constant volume, shake up to get.
L-100%: precision pipettes reference substance stock solution 3-1 to the 10ml capacity under 1.0ml " (1) reference substance solution " item
Bottle, to dilute dilution agent and constant volume, shake up to get.
L-80%: precision pipettes reference substance stock solution 3-1 to the 10ml volumetric flask under 0.8ml " (1) reference substance solution " item,
To dilute dilution agent and constant volume, shake up to get.
L-50%: precision pipettes reference substance stock solution 3-1 to the 10ml volumetric flask under 0.5ml " (1) reference substance solution " item,
To dilute dilution agent and constant volume, shake up to get.
L-LOQ: the quantitative limit solution of " taking (3) detection limit and quantitative limit solution " under item to get.
By above-mentioned solution through 0.22um nylon membrane filtration after sample introduction 1ul, by following (5) chromatographic condition progress gas-chromatography
It is tested and analyzed with mass spectrometric hyphenated technique.
(5) chromatographic condition
Use using 100% polyethylene glycol as stationary phase capillary column (Agilent VF-WAXms, 30m × 0.25mm ×
0.25 μm), 200 DEG C of injector temperature, temperature program: 90 DEG C run 5 minutes, are warming up to 210 DEG C per minute with 25 DEG C, operation
It 2.2 minutes, flow velocity 1.0ml/min, is run 2 minutes, sample volume 1ul, split ratio 20:1 under the conditions of 230 DEG C, carrier gas
For helium, detector is mass detector, acquisition mode are as follows: SIM mode, quota ion 61/65/78, and solvent delay 3 minutes,
The detector shut-in time 9 minutes.
In table 1, the blank solvent refers specifically to ethanol solution；
The test sample refers specifically to source of people retinal pigment epithelium injection (lot number 201805029RCY-QC1), warp
Supernatant is taken after centrifugation；
9 parts of solution specifically refers to:
1, precision weighs test sample about 25mg, is placed in 5ml volumetric flask, and 0.25ml is added thereto, and " (1) reference substance is molten
Reference substance stock solution 3-1 under liquid " item adds diluent dissolution and constant volume, shakes up, filtered with 0.22um nylon membrane to get in parallel
Prepare three parts.
2, precision weighs test sample about 25mg, is placed in 5ml volumetric flask, and 0.5ml " (1) reference substance solution " is added thereto
Reference substance stock solution 3-1 under adds diluent dissolution and constant volume, shakes up, filtered with 0.22um nylon membrane to get matching in parallel
Three parts of system.
3, precision weighs test sample about 25mg, is placed in 5ml volumetric flask, and 0.75ml is added thereto, and " (1) reference substance is molten
Reference substance stock solution 3-1 under liquid " item adds diluent dissolution and constant volume, shakes up, filtered with 0.22um nylon membrane to get in parallel
Prepare three parts.
6 parts of solution specifically refers to:
Precision weighs test sample about 25mg, is placed in 5ml volumetric flask, and 0.5ml " (1) reference substance solution " item is added thereto
Under reference substance stock solution 3-1, add diluent dissolution and constant volume, shake up, filtered with 0.22um nylon membrane to get preparing 6 in parallel
Part；
The test sample mark-on solution specifically refers to: a certain amount of reference substance deposit is added in configured test solution
Liquid；
The RSD is relative standard deviation, exactly refers to standard deviation (SD)/calculated result arithmetic mean of instantaneous value × 100%；
The C (ug/ml) is the concentration unit of sample；
The S/N is the signal-to-noise ratio of chromatogram baseline；
The initial temperature are as follows: detector by the way of temperature programming, just be temperature be temperature programming at the beginning when
Temperature.
Eventually by the verification results such as detection limit, quantitative limit, the range of linearity are calculated, it is shown in Table 1 (verification result table)
1 verification result table of table
From the data of table 1 it can be seen that
Detection limit and quantitative limit are extremely low, 0.0100ug/ml and 0.0501ug/ml are respectively reached, so as to delicately examine
Measure remaining dimethyl sulfoxide in sample.
The correlation coefficient r of linear equation reaches that the rate of recovery mean value of 0.9999,9 parts of solution is 104% and its RSD is
2%, the accuracy for more demonstrating this method is high.
Duplicate measurements comparison is carried out with 6 parts of solution duplicate measurements and with distinct device, different personnel, the rate of recovery
Mean value and RSD respectively reach 103%, 1% and 97%, 4%, more demonstrate the favorable reproducibility of this method, and precision is high.
The research of test sample and reference substance standing time, illustrating it, dimethyl sulfoxide survey ingredient will not within standing time
Degradation, can be tested within the time of placement.Because of gas-chromatography and mass spectrograph category precision detecting system, by the external world influenced compared with
Greatly, such as temperature, humidity, vibration, voltage instability factor factor, now by changing chromatographic condition by a small margin, the detection method
It still is able to reach the requirement of detection, illustrates that the durability of this method is good.
Embodiment 2
Source of people retinal pigment epithelium injection, manufacturer are the limited public affairs of Jiangsu Ai Erkang biological medicine science and technology
Department, lot number: 201708093RCY-1；Using dimethyl sulfoxide chromatographic isolation and mass spectrometry quantitative analysis method:
Dimethyl sulphoxide control product make solvent with ethyl alcohol and are configured
Reference substance stock solution 1-1: taking dimethyl sulphoxide control product 51.36mg to be placed in 10ml volumetric flask, with ethyl alcohol constant volume,
And it shakes up.
Reference substance stock solution 1-2: taking dimethyl sulphoxide control product 50.48mg to be placed in 10ml volumetric flask, with ethyl alcohol constant volume,
And it shakes up.
Reference substance stock solution 2-1: precision pipettes 0.5ml reference substance stock solution 1-1 in 50ml volumetric flask respectively, uses ethyl alcohol
Constant volume, and shake up.
Reference substance stock solution 2-2: precision pipettes 0.5ml reference substance stock solution 1-2 in 50ml volumetric flask respectively, uses ethyl alcohol
Constant volume, and shake up.
Reference substance stock solution 3-1: precision pipettes 2.0ml reference substance stock solution 2-1 in 20ml volumetric flask respectively, uses ethyl alcohol
Constant volume, and shake up.
Reference substance stock solution 3-2: precision pipettes 2.0ml reference substance stock solution 2-2 in 20ml volumetric flask respectively, uses ethyl alcohol
Constant volume, and shake up.
Reference substance solution configuration:
Reference substance solution 1: precision pipettes 1.0ml reference substance stock solution 3-1 and sets in 10ml volumetric flask, with ethyl alcohol constant volume, and
It shakes up to get reference substance solution 1 (STD1).
Reference substance solution 2: precision pipettes 1.0ml reference substance stock solution 3-2 and sets in 10ml volumetric flask, with ethyl alcohol constant volume, and
It shakes up to get reference substance solution 2 (STD2).
Test solution configuration:
Take source of people retinal pigment epithelium injection into centrifuge tube, be centrifuged 5 minutes under conditions of 702g, take from
Supernatant after the heart is to get sample to be tested.
Test solution SPL-1: precision weighs sample to be tested 25.39mg into 5ml volumetric flask, with ethyl alcohol constant volume, shakes up
To obtain the final product.
Test solution SPL-2: precision weighs sample to be tested 26.68mg into 5ml volumetric flask, with ethyl alcohol constant volume, shakes up
To obtain the final product.
By above-mentioned test solution SPL-1, SPL-2 and reference substance solution 1,2 after the filtration of 0.22um nylon membrane sample introduction
1ul carries out analysis detection with internal standard method for gas chromatography technology.According to reference substance peak area, reference substance concentration, test sample peak
Area, test sample sampling amount and extension rate calculate the content of dimethyl sulfoxide in test sample.
Its chromatographic condition are as follows: use using 100% polyethylene glycol as stationary phase capillary column (Agilent VF-WAXms, 30
M × 0.25mm × 0.25 μm), 200 DEG C of injector temperature, temperature program: 90 DEG C run 5 minutes, are warming up to per minute with 25 DEG C
It 210 DEG C, runs 2.2 minutes, flow velocity 1.0ml/min, is run 2 minutes under the conditions of 230 DEG C, sample volume is 1 μ l, and split ratio is
20:1, carrier gas are helium, and detector is mass detector, acquisition mode are as follows: SIM mode, quota ion 61/65/78, solvent prolongs
Slow 3 minutes, the detector shut-in time 9 minutes.
Embodiment 3
Source of people retinal pigment epithelium injection, manufacturer are the limited public affairs of Jiangsu Ai Erkang biological medicine science and technology
Department, lot number: 201710097RCY-1, using dimethyl sulfoxide chromatographic isolation and mass spectrometry quantitative analysis method:
The configuration process of reference substance solution 1,2 is the same as embodiment 2
Test solution configuration:
Take source of people retinal pigment epithelium injection into centrifuge tube, be centrifuged 5 minutes under conditions of 702g, take from
Supernatant after the heart is to get sample to be tested.
Test solution SPL-3: precision weighs sample to be tested 24.08mg into 5ml volumetric flask, with ethyl alcohol constant volume, shakes up
To obtain the final product.
Test solution SPL-4: precision weighs sample to be tested 25.31mg into 5ml volumetric flask, with ethyl alcohol constant volume, shakes up
To obtain the final product.
By above-mentioned test solution SPL-3, SPL-4 and reference substance solution 1,2 after the filtration of 0.22um nylon membrane sample introduction
1ul carries out analysis detection with internal standard method for gas chromatography technology.According to reference substance peak area, reference substance concentration, test sample peak
Area, test sample sampling amount and extension rate calculate the content of dimethyl sulfoxide in test sample.
Chromatographic condition is with embodiment 2, and only SPL-3, SPL-4 are different from embodiment 2.
Embodiment 4
Source of people retinal pigment epithelium injection, manufacturer are the limited public affairs of Jiangsu Ai Erkang biological medicine science and technology
Department, lot number: 201712121RCY-4, using dimethyl sulfoxide chromatographic isolation and mass spectrometry quantitative analysis method:
The configuration process of reference substance solution 1,2 is the same as embodiment 2.
Test solution configuration:
Take source of people retinal pigment epithelium injection into centrifuge tube, be centrifuged 5 minutes under conditions of 702g, take from
Supernatant after the heart is to get sample to be tested.
Test solution SPL-5: precision weighs sample to be tested 26.00mg into 5ml volumetric flask, with ethyl alcohol constant volume, shakes up
To obtain the final product.
Test solution SPL-6: precision weighs sample to be tested 25.89mg into 5ml volumetric flask, with ethyl alcohol constant volume, shakes up
To obtain the final product.
By above-mentioned test solution SPL-5, SPL-6 and reference substance solution 1,2 after the filtration of 0.22um nylon membrane sample introduction
1ul carries out analysis detection with internal standard method for gas chromatography technology.According to reference substance peak area, reference substance concentration, test sample peak
Area, test sample sampling amount and extension rate calculate the content of dimethyl sulfoxide in test sample.
Chromatographic condition is with embodiment 2, and only SPL-5, SPL-6 are different from embodiment 2.
The content for detecting dimethyl sulfoxide in the source of people retinal pigment epithelium injection of different batches is as shown in table 2
2 different batches source of people retinal pigment epithelium injection dimethyl sulfoxide content of table
| Batch | 201708093RCY-1 | 201710097RCY-1 | 201712121RCY-4 |
| Content | 0.0001% is less than LOQ | It is not detected | It is not detected |
The advantages of the method for the present invention, essentially consists in its detection limit and quantitative limit is very low, is that conventional method is unable to reach
's.It, can be quasi- as long as the dimethyl sulfoxide with the presence of denier remains from the point of view of the testing result of three batch samples in table 2
Really it detected.
Line of the analysis method of the present invention to dimethyl sulfoxide content detection in source of people retinal pigment epithelium injection
Property range, accuracy, precision, detection limit and the test results such as quantitative limit show that established analysis method can be good at
Measurement applied to dimethyl sulfoxide content in different practical biological samples.
Claims (10)
1. the remaining measuring method of dimethyl sulfoxide in a kind of source of people retinal pigment epithelium injection, which is characterized in that
In the measuring method, the centrifuged supernatant obtained using after the centrifugation of source of people retinal pigment epithelium injection is as to test sample
Product use organic reagent to divide the sample to be tested and reference substance as diluent using dimethyl sulphoxide control product as external standard
It is not diluted, after membrane filtration, carries out analysis detection using internal standard method for gas chromatography technology.
2. measuring method according to claim 1, which is characterized in that the measuring method the following steps are included:
(1) preparation of sample to be tested: source of people retinal pigment epithelium injection is transferred in centrifuge tube, centrifugation, take from
Supernatant after the heart adds diluent to be diluted up to sample to be tested；
(2) preparation of reference substance solution: dimethyl sulphoxide control product are weighed into volumetric flask, adds diluent gradient dilution, obtains
Reference substance solution；
(3) analysis detection: firstly, the reference substance solution that step (2) is obtained through membrane filtration, then utilizes gas-chromatography and matter
Spectrum joint technology is measured, and obtains standard calibration curve；Then sample to be tested step (1) obtained after membrane filtration,
Analysis detection is carried out using internal standard method for gas chromatography technology, then passes through the standard calibration curve and calculation formula meter
Calculate the residual quantity of dimethyl sulfoxide in sample to be tested；
If in continuous mode, the concentration of the sample to be tested exceeds the range of linearity of the reference substance solution, then need to described
Product to be tested is diluted.
3. measuring method according to claim 1, which is characterized in that the diluent is methanol, acetonitrile, one in ethyl alcohol
Kind is several.
4. measuring method according to claim 1 or 2, which is characterized in that contain inorganic salts in the sample to be tested, including
Magnesium chloride, calcium chloride, sodium acetate, sodium citrate.
5. measuring method according to claim 2, which is characterized in that in step (1), the condition of the centrifugation is 500g-
1500g is centrifuged -15 minutes 2 minutes；And/or in step (2), the diluent is one of methanol, acetonitrile, ethyl alcohol or several
Kind.
6. measuring method according to claim 2, which is characterized in that the calculation formula is as follows:
In formula: WSTDDMSO mass mg in=reference substance,
WSPLThe quality mg of=sample to be tested,
BSTDThe average peak area of DMSO in=reference substance,
ASPLThe peak area of DMSO in=sample to be tested,
VSPL=sample to be tested dilutes volume ml,
VSTD=reference substance dilutes volume ml,
PSTDThe content % of=reference substance.
7. measuring method according to claim 1 or 2, which is characterized in that the internal standard method for gas chromatography technology into
The condition of row analysis detection are as follows: the condition of gas-chromatography: in capillary chromatographic column, sample volume is 0.5 μ l-2 μ l, injection port temperature
Degree is 160 DEG C -260 DEG C, and flow velocity 0.5ml/min-1.5ml/min, split ratio 15-25:1, carrier gas is helium, using program
Heating；Detector is mass spectrometer detector: acquisition mode be SIM mode, quota ion 61/65/78, solvent delay 1 minute -5
Minute, the detector shut-in time is -20 minutes 6 minutes.
8. measuring method according to claim 7, which is characterized in that the condition of described program heating are as follows: 70 DEG C -120 DEG C
Then operation -10 minutes 3 minutes rises to 170 DEG C -240 DEG C with 20 DEG C -35 DEG C per minute, run 1-5 minutes；Then 200
It is run -6 minutes 2 minutes at DEG C -280 DEG C.
9. measuring method according to claim 2, which is characterized in that in step (3), the filter membrane includes nylon membrane, gathers
One or more of tetrafluoroethylene, PVDF membrane；The aperture of the filter membrane be 0.22 μm, 0.3 μm, 0.45 μm or
0.65μm。
10. application of the measuring method as described in claim 1 in measurement cell class product in dimethyl sulfoxide residual.
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