CN110184206B - Chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof - Google Patents
Chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof Download PDFInfo
- Publication number
- CN110184206B CN110184206B CN201910219755.3A CN201910219755A CN110184206B CN 110184206 B CN110184206 B CN 110184206B CN 201910219755 A CN201910219755 A CN 201910219755A CN 110184206 B CN110184206 B CN 110184206B
- Authority
- CN
- China
- Prior art keywords
- petroleum hydrocarbon
- tph2
- achromobacter
- hydrocarbon degrading
- chromium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/025—Achromobacter
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Environmental & Geological Engineering (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
A chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof relate to petroleum hydrocarbon degrading bacteria. Has been preserved in China center for type culture Collection in 2018, 11 months and 12 days, and the preservation number of the preservation center is CCTCC NO: m2018786. Provides a strain of chromium-resistant petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 for treating diesel oil and heavy metal Cr6+Application in contaminated soil treatment. The separation and application of the petroleum hydrocarbon degrading bacteria (Achromobacter insidious) Tph2-23 effectively fills the blank of research in the aspect, provides a set of feasible practical scheme for the treatment work of actual polluted environment, and shows huge actual application prospect.
Description
Technical Field
The invention relates to a petroleum hydrocarbon degrading bacterium, in particular to a chromium-resistant petroleum hydrocarbon degrading bacterium (Achromobacter insidatus) Tph2-23 and application thereof.
Background
The diesel oil is a light petroleum product, and is a mixture of complex hydrocarbons (with carbon atoms of about 10-22), and is divided into light diesel oil (with a boiling point range of about 180-370 ℃) and heavy diesel oil (with a boiling point range of about 350-410 ℃). The most important use of diesel is in diesel engines for vehicles and ships. Petroleum hydrocarbon pollutants represented by diesel oil inevitably pollute the environment in the processes of transportation, loading, unloading, processing and use. They are flammable and the exhaust gases produced after combustion contain nitrogen oxides, carbon monoxide, carbon dioxide, aldehydes and a large amount of black smoke, which cause serious pollution to the atmospheric environment (Khan, Samiya, Sanjay Gupta & Nidhi gupta.2018. purification of diesel oil using Pseudomonas sp. biotechnology letters.1573-6776). Once a leakage event occurs, the diesel oil floats on the surface of the sea and forms an oil film, which can migrate and transform through diffusion, evaporation, dissolution, emulsification, photodegradation, biodegradation and absorption. In the process, the diesel oil can be attached to the fish gills to suffocate the fish; inhibiting the oviposition and incubation of waterfowl and destroying the water permeability of feathers of the waterfowl; reducing the quality of aquatic products, and the like. The formed oil film hinders the reoxygenation of the water body, influences the growth of marine plankton and destroys the marine ecological balance. In addition, the seaside landscape can be damaged, the tourism environment of the seaside landscape is influenced, the organic matter composition and the structure of the soil can be changed after the seaside landscape enters the soil, and the growth, the propagation and the distribution change of microorganisms in the soil are influenced; a large amount of petroleum can block soil pores and has an inhibiting effect on the activity of enzymes in soil; and can enter human body through biological amplification and aggregation of food chain, and affect normal functions of organs such as liver and kidney, even cause canceration, thereby endangering human health. In 2017, 10 and 27, the national institutes of health (IARC) publishes a list of carcinogens for preliminary reference, and diesel oil (light fraction) is classified into a list of 3 types of carcinogens.
Chromium (Cr) is a heavy metal element widely used in industrial production. According to the annual report of environment statistics of China 2012 published by the environmental protection department of the people's republic of China, the year 2012 indicates that hexavalent chromium (Cr) is contained in industrial wastewater6+) And total chromium emissions were 70.4 tons and 188.6 tons, respectively. Cr (chromium) component6+Because of strong oxidizing property and corrosiveness and the capability of permeating biological membrane, the biological membrane can easily enter cells and has strong toxic effect on human bodies, and the toxicity is about Cr 3+100 times of toxicity, mutagenicity about Cr3+1000 times (ZHAO Ran #, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. biomedication of Hexavalent Chromium polarization by Sporosarcina saromensis M52Isolated from offset settings in Xiamen, China.2016, Biomed Environ Sci,29(2): 127-. Not only that, Cr6+For swallowing toxicSubstance/inhalation toxicants, skin contact may lead to sensitization; more likely to cause genetic defects, possibly carcinogenic inhalation, and persistent environmental risks (Pajor F, P Lo Ti P, B < rdos L. Accumulation of sodium heavymetals (Pd, Cd and Cr) in milk of grazing sheath in not-easy-eat Hungary.2012, Food Sci Biotechnol,2(1):389 394.). Cr has been determined by the International agency for research on cancer (IARC) and the American society of government Industrial hygienists (ACGIH)6+The compounds have carcinogenic properties. Therefore, research into Cr6+The pollution treatment technology has great significance for ecological environment protection and human health.
Research shows that petroleum hydrocarbon polluted soil, such as oil field soil, thermal power plant, etc., is often accompanied by heavy metal contamination (Fu, Xiaowen, Zhaojie Cui & Guolong zang.2014. distribution, distribution and distribution of heavy metals in oil-polarized soil after fermentation by oil extraction. Environmental Science: Processes & expressions 16.1737-44.) while part of heavy metal contamination has a lethal effect on microorganisms, and thus most of efficient petroleum hydrocarbon contamination remediation microorganisms have greatly reduced degradation efficiency under the above conditions (Dong, Zhi-Yong, Wen-Hui Huang, Ding-Feng & Hong-reforming. 2013. moisture-condensation and 260.399. evaluation of calcium-contamination).
At present, diesel oil and Cr are respectively contained6+Physical, chemical, biological and combination of different methods for soil remediation of pollutants have become common. However, the research on petroleum hydrocarbon pollutant-heavy metal combined pollution mainly takes ecological toxicological effects as a research. The reported in-situ remediation technologies for organic matter-heavy metal combined pollution include electric enhanced remediation, microbial remediation, phytoremediation and chemical-biological combined technology (chemical oxidation-microbial degradation, chemical reduction-super-accumulation plant enrichment and chemical passivator-phytoremediation), and no related report is found for specially aiming at diesel oil-Cr6+Composite pollution is a typical microbial remediation technology of toxic heavy metals, which are petroleum hydrocarbon pollutants.
Disclosure of Invention
The first purpose of the invention is to provide a strain of chromium-resistant petroleum hydrocarbon degrading bacteria (Achromobacter insidatus) Tph 2-23.
The second purpose of the invention is to provide a strain of chromium-resistant petroleum hydrocarbon degrading bacteria (Achromobacter inolities) Tph2-23 for treating diesel oil and heavy metal Cr6+Application in contaminated soil treatment.
The chromium-resistant petroleum hydrocarbon degrading bacterium (Achromobacter insidious) Tph2-23 is screened from a petroleum hydrocarbon polluted area (E113 degrees 55 ', N22 degrees 20') of an Australian power plant, and a soil sample is subjected to aseptic collection, subpackaging and sealing and then is conveyed to a laboratory at a low temperature for the next-stage functional strain separation and screening.
Under laboratory conditions: cleaning a 250mL conical flask, adding 90mL deionized water, adding 25 glass beads with the diameter of 0.5cm, sealing, placing in an autoclave, and sterilizing at 121 deg.C for 20 min. After the temperature is cooled to room temperature, 10.0g of the soil sample is added into a conical flask in a super clean bench, and the conical flask is sealed again and then placed in a constant temperature shaking table at 28 ℃ to shake for about 30 min. The suspension produced in the process is the stock solution of gradient dilution. The stock solution was diluted 10-fold at 10-fold-4、10-5、10-6To dilute the final concentration, the solution was spread evenly on an LB solid plate, and the plate was then inverted and incubated in a 28 ℃ incubator for about 48 hours. And repeatedly separating and purifying to obtain pure single bacterium petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph 2-23.
The similarity of the 16s rDNA sequence of the identified petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 and the model strain chromobacterium inolus DSM 23807(T) is 99.67%, and the identification of phylogenetic science proves that the strain is classified into the Achromobacter inolus and is named as the petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph 2-23. Petroleum hydrocarbon degrading bacteria (Achromobacter inolitis) Tph2-23 was deposited in the China center for type culture Collection on 11/12 of 2018, with the following addresses: wuhan university in Wuhan, China, zip code: 430072, preservation number of preservation center is CCTCC NO: m2018786.
In LB liquid MediumInoculating a single colony of petroleum hydrocarbon degrading bacteria (Achromobacter insidious) Tph2-23, and culturing overnight to obtain seed liquid. Respectively sterilizing 10g diesel oil and 90mL inorganic salt liquid culture medium, mixing, and adding dried potassium dichromate powder to constant weight to make Cr in the system6+The concentration is 50mg/L (the value is determined by preliminary experiments in the early stage), the seed solution is added according to the proportion of 1 percent (namely 1mL), the mixture is cultured for 15 days at the temperature of 28 ℃ and at the rpm of 150, and the Cr in the reaction system is dynamically monitored at the time interval of 3 days in the culture process6+And the content of the diesel oil, and calculating the degradation rate of the diesel oil at the corresponding time point. Then 50mg/LCr6+For preparing Cr-containing material in gradient6+The LB liquid medium of (1), inoculated with a single colony of Petroleum Hydrocarbon-degrading bacteria (Achromobacter insoletus) Tph2-23, and subjected to plate-coating verification to determine that it is the highest in Cr tolerance6+And (4) concentration.
The experiment verifies that: the petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 of the invention is at pH7.6 and 28 ℃ and contains about 50mg/L Cr6+And 10% diesel oil inorganic salt liquid culture medium, culturing at 150rpm for 15 days, and treating Cr6+The removal rate reaches 88.7 percent, and 83.4 percent of diesel oil in a reaction system can be degraded. In Cr6+In the tolerance test, the highest content of petroleum hydrocarbon degrading bacteria (Achromobacter insidatus) Tph2-23 can be about 250mg/LCr6+The LB liquid medium of (1).
The LB solid medium comprises the following components: 10g of tryptone, 5g of yeast extract powder, 10g of sodium chloride, 15g/L of agar powder, 1L of deionized water and pH of 6.9-7.1. The LB liquid medium comprises the following components: 10g of tryptone, 5g of yeast extract powder, 10g of sodium chloride, 1L of deionized water and pH of 6.9-7.1. The inorganic salt liquid culture medium comprises the following components: 2.0g of calcium chloride, and preparing 1000 times of calcium chloride solution after the volume is fixed to 100 mL; magnesium sulfate heptahydrate 2.0g, constant volume to 100mL, prepared 100 x magnesium sulfate solution; 0.5g of ammonium sulfate, 0.5g of sodium nitrate, 1.0g of monopotassium phosphate, 1.0g of hydrated sodium dihydrogen phosphate and 800mL of deionized water, adjusting the pH value to 7.0-7.2, sterilizing under high pressure, adding a calcium chloride solution and a magnesium sulfate solution in a sterile environment according to a ratio, and adding sterile water to make up to 1L.
Therefore, the separation and application of the petroleum hydrocarbon degrading bacteria (Achromobacter insidious) Tph2-23 effectively fills the blank of research in the aspect, provides a set of feasible practical scheme for the treatment work of actual polluted environment, and shows huge actual application prospect.
Drawings
FIG. 1 shows the oil-microscopic appearance of the petroleum hydrocarbon-degrading bacterium (Achromobacter insoletus) Tph2-23 of the present invention.
FIG. 2is a phylogenetic tree of the petroleum hydrocarbon-degrading bacterium (Achromobacter insoletus) Tph2-23 of the present invention. In fig. 2, a scale indicates the disparity accuracy.
FIG. 3 shows the degradation rate of the petroleum hydrocarbon degrading bacteria (Achromobacter insidatus) Tph2-23 for hexavalent chromium and diesel oil at different times. In FIG. 3, a is hexavalent chromium and b is diesel.
The specific implementation mode is as follows:
the following examples will further illustrate the present invention with reference to the accompanying drawings.
Example 1: morphological characteristics of petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23
And streaking and inoculating the single colony to an LB solid culture medium, inverting the flat plate in a constant-temperature incubator, and culturing for 48 hours at 28 ℃, wherein the colony is circular, is beige and semitransparent, has a smooth and moist surface, regular edges, no halo, a central micro-bulge and a diameter of 0.5-1 mm, and is shown in figure 1.
Example 2: screening and identification of petroleum hydrocarbon degrading bacteria (Achromobacter inolitis) Tph2-23
(1) The soil samples were screened from a petroleum hydrocarbon contaminated area (E113 ° 55 ', N22 ° 20') of the australian power plant, aseptically collected, packaged, sealed, and transported to the laboratory at low temperature for the next stage of research.
(2) Under laboratory conditions: cleaning a 250mL conical flask, adding 90mL deionized water, adding 25 glass beads with the diameter of 0.5cm, sealing, placing in an autoclave, and sterilizing at 121 deg.C for 20 min.
(3) And after the system is cooled to the room temperature, adding 10.0g of the soil sample into a conical flask in an aseptic ultra-clean workbench, sealing the conical flask again, and then placing the conical flask in a constant-temperature shaking table at 28 ℃ to shake for about 30min so as to completely separate out microorganisms in soil pores.The suspension produced in the above process is the stock solution of gradient dilution. The stock solution was diluted 10 times at an equal ratio of 10-4、10-5、 10-6To dilute the final concentration, the solution was spread evenly on an LB solid plate, which was then placed upside down in an incubator at 28 ℃ for about 48 hours. Repeatedly separating and purifying to obtain single bacterium petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph 2-23.
(4) Extracting genome DNA of petroleum hydrocarbon degrading bacteria (Achromobacter insidius) Tph2-23, amplifying a 16SrDNA sequence by taking the genome DNA as a template, and performing similarity comparison analysis on the obtained 16S rDNA sequence and the existing 16S rDNA nucleic acid sequence in a database through EZbiocloud (https:// www.ezbiocloud.net /). Phylogenetic analyses were performed using Mega5.0 software after alignment with ClustalX. A phylogenetic tree of petroleum hydrocarbon-degrading bacteria (Achromobacter insidatus) Tph2-23 (neighbor-joining tree) is shown in FIG. 2. Through identification, the similarity of the 16s rDNA sequence of the petroleum hydrocarbon degrading bacteria (Achromobacter inotus) Tph2-23 and the model strain Achromobacter inotus DSM 23807(T) is 99.67%, and the sequence and the model strain form a branch together, so that the strain can be judged to be classified into Achromobacter inotus and named as the petroleum hydrocarbon degrading bacteria (Achromobacter inotus) Tph 2-23. Petroleum hydrocarbon degrading bacteria (Achromobacter inolitis) Tph2-23 was deposited in the China center for type culture Collection on 11/12 of 2018, with the following addresses: wuhan university in Wuhan, China, zip code: 430072, preservation number of preservation center is CCTCC NO: m2018786.
Example 3: petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 for Cr6+And determination of diesel degradation rate
(1) Preparing an LB liquid culture medium, selecting a single colony of petroleum hydrocarbon degrading bacteria (Achromobacter inolitis) Tph2-23 on an LB solid plate, inoculating the single colony in the LB liquid culture medium, and culturing overnight to obtain Tph2-23 seed liquid.
(2) Respectively sterilizing 10g diesel oil and 90mL inorganic salt liquid culture medium, mixing, and adding dried potassium dichromate powder to make Cr in the system constant6+About 50 mg/L. Inoculating strain seed solution at a ratio of 1%, culturing at 28 deg.C and 150rpm for 15d, and extracting every 3dSample, determination of Cr6+And diesel oil content, and calculating the degradation rate of the corresponding time point.
(3) The results show that: petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 for Cr6+The degradation rate is up to 88.7%, and 83.4% of diesel oil in the reaction system can be degraded.
Example 4: petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 for Cr6+Preparing an LB liquid culture medium by the highest tolerance concentration detection (1), selecting a single colony of petroleum hydrocarbon degrading bacteria (Achromobacter inolitis) Tph2-23 on an LB solid plate, inoculating the single colony in the LB liquid culture medium, and culturing overnight to obtain the Tph2-23 seed liquid.
(2) At 50mg/L Cr6+Preparing Cr with different concentrations for gradient intervals6+Inoculating Tph2-23 seed solution, monitoring whether the absorbance value in the culture medium changes after a certain culture period and performing LB solid plate coating verification to determine the highest tolerable Cr6+And (4) concentration.
(3) The results show that: petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 can tolerate 250mg/LCr at most6+。
The LB solid medium comprises the following components: 10g of tryptone, 5g of yeast extract powder, 10g of sodium chloride, 15g/L of agar powder, 1L of deionized water and pH of 6.9-7.1. The LB liquid medium comprises the following components: 10g of tryptone, 5g of yeast extract powder, 10g of sodium chloride, 1L of deionized water and pH of 6.9-7.1. The inorganic salt liquid culture medium comprises the following components: 2.0g of calcium chloride, and preparing 1000 times of calcium chloride solution after the volume is fixed to 100 mL; magnesium sulfate heptahydrate 2.0g, constant volume to 100mL, prepared 100 x magnesium sulfate solution; 0.5g of ammonium sulfate, 0.5g of sodium nitrate, 1.0g of monopotassium phosphate, 1.0g of hydrated sodium dihydrogen phosphate and 800mL of deionized water, adjusting the pH value to 7.0-7.2, sterilizing under high pressure, adding a calcium chloride solution and a magnesium sulfate solution in a sterile environment according to a ratio, and adding sterile water to make up to 1L.
The degradation rates of the petroleum hydrocarbon degrading bacteria (Achromobacter inolus) Tph2-23 on hexavalent chromium and diesel oil at different times are shown in figure 3.
Sequence listing
<110> university of mansion
<120> chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1395
<212> DNA
<213> Achromobacter insolitus Tph2-23
<400> 1
acatgcagtc gaacggcagc acggacttcg gtctggtggc gagtggcgaa cgggtgagta 60
atgtatcgga acgtgcccag tagcggggga taactacgcg aaagcgtagc taataccgca 120
tacgccctac gggggaaagc aggggatcgc aagaccttgc actattggag cggccgatat 180
cggattagct agttggtggg gtaacggctc accaaggcga cgatccgtag ctggtttgag 240
aggacgacca gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagtg 300
gggaattttg gacaatgggg gaaaccctga tccagccatc ccgcgtgtgc gatgaaggcc 360
ttcgggttgt aaagcacttt tggcaggaaa gaaacgtcgc gggttaatac cccgcggaac 420
tgacggtacc tgcagaataa gcaccggcta actacgtgcc agcagccgcg gtaatacgta 480
gggtgcaagc gttaatcgga attactgggc gtaaagcgtg cgcaggcggt tcggaaagaa 540
agatgtgaaa tcccagagct taactttgga actgcatttt taactaccgg gctagagtgt 600
gtcagaggga ggtggaattc cgcgtgtagc agtgaaatgc gtagatatgc ggaggaacac 660
cgatggcgaa ggcagcctcc tgggataaca ctgacgctca tgcacgaaag cgtggggagc 720
aaacaggatt agataccctg gtagtccacg ccctaaacga tgtcaactag ctgttggggc 780
cttcgggcct tggtagcgca gctaacgcgt gaagttgacc gcctggggag tacggtcgca 840
agattaaaac tcaaaggaat tgacggggac ccgcacaagc ggtggatgat gtggattaat 900
tcgatgcaac gcgaaaaacc ttacctaccc ttgacatgtc tggaattccg aagagatttg 960
gaagtgctcg caagagaacc ggaacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt 1020
gagatgttgg gttaagtccc gcaacgagcg caacccttgt cattagttgc tacgaaaggg 1080
cactctaatg agactgccgg tgacaaaccg gaggaaggtg gggatgacgt caagtcctca 1140
tggcccttat gggtagggct tcacacgtca tacaatggtc gggacagagg gtcgccaacc 1200
cgcgaggggg agccaatccc agaaacccga tcgtagtccg gatcgcagtc tgcaactcga 1260
ctgcgtgaag tcggaatcgc tagtaatcgc ggatcagcat gtcgcggtga atacgttccc 1320
gggtcttgta cacaccgccc gtcacaccat gggagtgggt tttaccagaa gtagttagcc 1380
taaccgtaag gaggg 1395
Claims (3)
1. A chromium-resistant petroleum hydrocarbon degrading bacterium is characterized by being rare Achromobacter (Achromobacter inolus) Tph2-23, which is preserved in China Center for Type Culture Collection (CCTCC) in 2018, 11 months and 12 days, and the preservation number of the CCTCC NO: m2018786.
2. The use of the chromium-tolerant petroleum hydrocarbon-degrading bacterium of claim 1 in the treatment of diesel fuel.
3. The chromium-resistant petroleum hydrocarbon degrading bacterium as claimed in claim 1, which is cultured in the presence of heavy metal Cr6+The method is applied to the treatment of polluted soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910219755.3A CN110184206B (en) | 2019-03-22 | 2019-03-22 | Chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910219755.3A CN110184206B (en) | 2019-03-22 | 2019-03-22 | Chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110184206A CN110184206A (en) | 2019-08-30 |
| CN110184206B true CN110184206B (en) | 2021-09-28 |
Family
ID=67714014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910219755.3A Active CN110184206B (en) | 2019-03-22 | 2019-03-22 | Chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110184206B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937705A (en) * | 2014-03-10 | 2014-07-23 | 赵晗 | Achromobacter insolitus and application of Achromobacter insolitus in heavy metal ion removing |
-
2019
- 2019-03-22 CN CN201910219755.3A patent/CN110184206B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937705A (en) * | 2014-03-10 | 2014-07-23 | 赵晗 | Achromobacter insolitus and application of Achromobacter insolitus in heavy metal ion removing |
Non-Patent Citations (1)
| Title |
|---|
| Genome Sequencing Reveals the Potential of Achromobacter sp. HZ01 for Bioremediation;Yue-Hui Hong等;《Frontiers in Microbiology》;20170809;第1-14页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110184206A (en) | 2019-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Massaccesi et al. | Cadmium removal capacities of filamentous soil fungi isolated from industrially polluted sediments, in La Plata (Argentina) | |
| Mihdhir et al. | Detection, identification and characterization of some heavy metals tolerant bacteria | |
| CN109759436B (en) | Manganese-resistant providencia bacterial strain and method for repairing heavy metal contaminated soil | |
| CN106635858A (en) | Paracoccus sp. and culture application thereof | |
| CN110564640B (en) | Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof | |
| CN103013850A (en) | Salt-resistant alkali-resistant Cr(VI) reduction microbe and screening method thereof | |
| CN104498404A (en) | Bacillus CP-1 for lead-chromium pollution restoration and application of bacillus CP-1 | |
| Kafilzadeh et al. | Isolation and identification of chromium (VI)-resistant bacteria from Soltan Abad river sediments (Shiraz-Iran) | |
| CN114107092A (en) | Plant endophyte Gordonia L191 for degrading phthalate and application thereof | |
| CN118389361A (en) | Passivation heavy metal arsenic strain As01 and application thereof | |
| CN118374395A (en) | Passivation heavy metal arsenic strain As05 and application thereof | |
| CN114015599B (en) | Lead-resistant bacterium and application thereof | |
| CN110317741B (en) | Chromium-resistant petroleum hydrocarbon degrading bacterium Thp3-45A and application thereof | |
| CN110184206B (en) | Chromium-resistant petroleum hydrocarbon degrading bacterium Tph2-23 and application thereof | |
| CN115449489A (en) | Oil reducing bacteria and composite microbial inoculum thereof, preparation method and application | |
| CN110184207B (en) | Chromium-resistant petroleum hydrocarbon degrading bacterium M23A and application thereof | |
| Mishra et al. | Study on hexavalent chromium reduction by chromium resistant bacterial isolates of Sukinda mining area | |
| CN110255717B (en) | Degrading bacterium of strobilurin fungicide and application thereof | |
| CN110317742B (en) | Chromium-resistant petroleum hydrocarbon degrading strain Tph3-32 and application thereof | |
| CN110029072B (en) | Agrobacterium and application thereof in degradation of 3-hydroxypyridine | |
| CN115960745A (en) | Salt-tolerant epichlorohydrin degrading bacterium and application thereof in treatment of epichlorohydrin in high-salinity water | |
| CN112831434B (en) | Halophilic brevibacterium linum capable of efficiently degrading ammonia nitrogen and application thereof | |
| CN110283736B (en) | Chromium-resistant petroleum hydrocarbon degrading bacterium Tph1-14 and application thereof | |
| CN114657089A (en) | Chromium reducing bacillus and method for restoring chromium-polluted soil by using same | |
| Shekhar et al. | Detoxification hexavalent chromium by potential chromate reducing bacteria isolated from turnery effluent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |