CN110184191A - A kind of raising lactobacillus bulgaricus is stored at room temperature and the freeze drying protectant of anti-pressure ability and its application - Google Patents
A kind of raising lactobacillus bulgaricus is stored at room temperature and the freeze drying protectant of anti-pressure ability and its application Download PDFInfo
- Publication number
- CN110184191A CN110184191A CN201910482027.1A CN201910482027A CN110184191A CN 110184191 A CN110184191 A CN 110184191A CN 201910482027 A CN201910482027 A CN 201910482027A CN 110184191 A CN110184191 A CN 110184191A
- Authority
- CN
- China
- Prior art keywords
- freeze
- lactobacillus bulgaricus
- parts
- drying
- freeze drying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to probiotic composition preparation technical fields, and in particular to a kind of preparation process that lactobacillus bulgaricus can be improved and be stored at room temperature the freeze drying protectant and freeze-dried vaccine powder of ability and anti-pressure ability.Lactobacillus bulgaricus bacterium powder prepared by the present invention has high stability and extremely strong anti-pressure ability at normal temperature, viable bacteria loss can be especially significantly reduced in tablet process, improve product yield, it can be avoided the cold chain transportation of lactobacillus bulgaricus product, reduce its selling cost, its anti-pressure ability is improved simultaneously, reduces production cost.
Description
Technical field
The invention belongs to probiotic composition preparation technical fields, and in particular to lactobacillus bulgaricus room temperature can be improved in one kind
The freeze drying protectant and its freeze-dried vaccine powder preparation technique of hold capacity and anti-pressure ability.
Background technique
Lactobacillus bulgaricus (L.delbrueckii subsp.bulgaricus) is Lactobacillus delbrueckii
(L.delbrueckii) subspecies in kind, Gram-positive, negative catalase do not move, and no gemma is facultative to detest
Oxygen.DSShi lactobacillus subspecies bulgaricus is one of most important lactic acid fermented agent strain, during the fermentation can be through homotype cream
Lactose conversion is lactic acid by acid fermentation, and is peptides and amino acid by protein degradation, provides unique matter for dairy products
Ground, flavor, while assigning dairy products special dietotherapy effect, dairy products functionality and nutritive value are also improved, is being sent out
There is very important economic value in kefir milk industry.As people go deep into lactobacillus bulgaricus research, except can use
In outside ferment agent for sour milk, itself has and improve the immunity of the human body, adjust the important physiology such as intestinal flora, improvement function of intestinal canal
And healthcare function.Therefore, more and more the probiotic composition containing lactobacillus bulgaricus in terms of medical treatment, health care and nutrition
Obtain promotion and application.
Since lactobacillus bulgaricus itself is more fragile, it is highly prone to temperature, pH, gas componant, mechanicals efforts etc.
The influence of extraneous factor and cause number of viable reduce and effect reduce.To ensure that there is higher viable count in shelf life
And beneficial function, most probiotic products containing lactobacillus bulgaricus are needed through cold chain transportation and low-temperature storage
Mode is sold, and strong influence and limits the application mode and product effect of product, while improving product cost, into
And influence the sale of product.Further, since lactobacillus bulgaricus is poor to the tolerance of mechanical stress, in its preparation processing
Viable bacteria loss is larger in production process, leads to that the production cost increases, production efficiency decline.Therefore, lactobacillus bulgaricus is promoted
The promotion of manufacturing enterprise's production efficiency is related to the tolerance of environment and related probiotics preparation more preferably and is widely answered
With.
Chinese patent CN102952755A discloses a kind of freeze drying protectant of lactobacillus bulgaricus, by lactose 20~30
Part, 20~30 parts of skimmed milk powder, 3~5 parts of sodium ascorbate, 0.2~0.6 part of Macrogol 4000, polyvinylpyrrolidone 0.2
~0.8 part, 0.5~2.0 part of bovine serum albumin(BSA) and 100 parts of distilled water compositions.Since component is excessive, it is not very convenient to prepare,
Meanwhile its anti-pressure ability is not required during the preparation process.
Since lactobacillus bulgaricus bacterium powder is prepared by freeze-drying at this stage, screening and optimization
Frozen-dried protective agent prescription, adjustment parameters of freeze-drying process become promoted lactobacillus bulgaricus to environmental resistance the most directly and
Effective means.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the weakness that lactobacillus bulgaricus self tolerance is poor, provide one
The lactobacillus bulgaricus freeze drying protectant and its freeze-dried vaccine powder preparation technique of kind high activity, strong resistance, can be significant
That improves lactobacillus bulgaricus is stored at room temperature ability and anti-pressure ability.
To achieve the above object, the present invention, which provides one kind lactobacillus bulgaricus can be improved, is stored at room temperature ability and resistance to compression energy
The freeze drying protectant of power.The constituent of freeze drying protectant are as follows: pregelatinized starch, skimmed milk powder, maltodextrin and trehalose, it is remaining
Amount is water.Wherein pregelatinized starch has stronger water imbibition, is able to maintain that the low water activity state of bacterium powder, improves bacterium powder and is storing
And the stability in product.Skimmed milk powder, maltodextrin and trehalose can effectively mitigate Freeze-drying Damage and drying stress damage,
Improve the freeze-drying survival rate of thallus.
The present invention also provides a specific technical solution, each composition of freeze drying protectant is respectively as follows: pregelatinated by weight
80~120 parts of starch, 50~100 parts of skimmed milk powder, 80~100 parts of maltodextrin, 20~40 parts of trehalose, sterile distilled water
640~770 parts.
Another object of the present invention is to provide a kind of raising lactobacillus bulgaricus and is stored at room temperature ability and anti-pressure ability
Freeze-dried vaccine powder preparation technique, includes the following steps:
1. after lactobacillus bulgaricus is activated, carrying out amplification fermentation training step by step according to the inoculum concentration of 5-10% (v/v)
It supports, thallus is collected by centrifugation, according to bacterium mud: freeze drying protectant is that the weight ratio of 1:1~3 is sufficiently mixed, and is made
Machine liquid in freeze-drying.
2. upper machine liquid, which will be lyophilized, pours into freeze-drying pallet, upper machine liquid thickness control is in 0.5~2.0cm, at -35 DEG C~-45 DEG C
It is put into pre-freeze 4-8h in freeze dryer.
3. drying process vacuum degree controls between 0.1-0.3mbar always, primary drying process is according to 0.5~3 DEG C/h
Rate be warming up to 0 DEG C.Parsing-desiccation process is warming up to 30 DEG C from 0 DEG C according to the rate of 1~4 DEG C/h, maintains 2-4h.
4. after vacuum freeze drying, bacterium powder crush to be packed into seal in aluminium plastic bag and is stored and -20 DEG C.
The advantage of the invention is that by the optimization of screening and lyophilized technique to lactobacillus bulgaricus freeze drying protectant,
Being stored at room temperature for lactobacillus bulgaricus bacterium powder is realized, significantly improves it in the anti-pressure ability of subsequent tablet process,
Be conducive to steady production, improve production efficiency, reduces and produce and sell cost.
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified
Conventional method.Material as used in the following examples, bacterial strain, reagent etc., are commercially available unless otherwise specified.
The preparation of 1. freeze drying protectant of embodiment and freeze-dried vaccine powder
Each composition of freeze drying protectant is respectively as follows: 100 parts of pregelatinized starch, 50 parts of skimmed milk powder, maltodextrin by weight
80 parts, 20 parts of trehalose, 750 parts of sterile distilled water.
Freeze-dried vaccine powder preparation technique, after including the following steps: that lactobacillus bulgaricus is activated, according to 5-10% (v/
V) inoculum concentration is amplified fermented and cultured step by step, thallus is collected by centrifugation, according to bacterium mud: freeze drying protectant 1:2
Weight ratio sufficiently mixed, the upper machine liquid of freeze-drying is made.Upper machine liquid will be lyophilized and pour into freeze-drying pallet, upper machine liquid thickness control exists
1.5cm is put into pre-freeze 6h in freeze dryer at -40 DEG C.Drying process vacuum degree is controlled always in 0.2mbar, primary drying process
0 DEG C is warming up to according to the rate of 2 DEG C/h.Parsing-desiccation process is warming up to 30 DEG C from 0 DEG C according to the rate of 3 DEG C/h, maintains 3h.Very
After vacuum freecing-dry, bacterium powder crush to be packed into seal in aluminium plastic bag and deposits in -20 DEG C.
The preparation of 2. freeze drying protectant of embodiment and freeze-dried vaccine powder
Each composition of freeze drying protectant is respectively as follows: 120 parts of pregelatinized starch, 60 parts of skimmed milk powder, maltodextrin by weight
80 parts, 40 parts of trehalose, 700 parts of sterile distilled water.
Freeze-dried vaccine powder preparation technique, after including the following steps: that lactobacillus bulgaricus is activated, according to 5-10% (v/
V) inoculum concentration is amplified fermented and cultured step by step, thallus is collected by centrifugation, according to bacterium mud: freeze drying protectant 1:3
Weight ratio sufficiently mixed, the upper machine liquid of freeze-drying is made.Upper machine liquid will be lyophilized and pour into freeze-drying pallet, upper machine liquid thickness control exists
1.5cm is put into pre-freeze 4-8h in freeze dryer at -40 DEG C.Drying process vacuum degree controls between 0.2mbar always, primary dry
Dry process is warming up to 0 DEG C according to the rate of 2 DEG C/h.Parsing-desiccation process is warming up to 30 DEG C from 0 DEG C according to the rate of 3 DEG C/h, dimension
Hold 3h.After vacuum freeze drying, bacterium powder crush to be packed into seal in aluminium plastic bag and is stored and -20 DEG C.
3. lactobacillus bulgaricus bacterium powder of embodiment is stored at room temperature stability test
Lactobacillus bulgaricus bacterium powder with the preparation of existing bacterium powder production technology is control, freeze drying protectant composition: de-
180 parts of fat milk powder, 60 parts of lactose, 60 parts of yeast powder, 700 parts of sterile distilled water；Lyophilized technique: drying process vacuum degree is controlled always
System is between 0.2mbar, and -60 DEG C of pre-freezing temperature, pre-freeze time 10h, primary drying process is warming up to 0 according to the rate of 4 DEG C/h
℃.Parsing-desiccation process is warming up to 25 DEG C from 0 DEG C according to the rate of 2 DEG C/h, maintains 3h.
Investigate the stability that Examples 1 and 2 preparation bacterium powder is stored 8 weeks under the conditions of 25 DEG C.Its test result (table 1) is aobvious
Show, compared with the control group, the ability that is stored at room temperature of Examples 1 and 2 preparation bacterium powder significantly improves.
1 lactobacillus bulgaricus bacterium powder of table is stored at room temperature stability
4. lactobacillus bulgaricus bacterium powder compression test of embodiment
Lactobacillus bulgaricus bacterium powder with the preparation of existing bacterium powder production technology is control, freeze drying protectant composition: de-
180 parts of fat milk powder, 60 parts of lactose, 60 parts of yeast powder, 700 parts of sterile distilled water；Lyophilized technique: drying process vacuum degree is controlled always
System is between 0.2mbar, and -60 DEG C of pre-freezing temperature, pre-freeze time 10h, primary drying process is warming up to 0 according to the rate of 4 DEG C/h
DEG C, parsing-desiccation process is warming up to 25 DEG C from 0 DEG C according to the rate of 2 DEG C/h, maintains 3h.
Lactobacillus bulgaricus bacterium powder prepared by control group bacterium powder and embodiment 1,2 is according to 5 × 109The standard of CFU/g
It is mixed respectively with auxiliary material, tablet is prepared using high speed tablet press, its resistance to compression is investigated by Viable detection in tablet
Ability.The lactobacillus bulgaricus bacterium powder of the display of its test result (table 2), Examples 1 and 2 preparation has stronger resistance to compression energy
Power can significantly improve the yield of product.
2 lactobacillus bulgaricus bacterium powder anti-pressure ability of table
5. lactobacillus bulgaricus bacterium powder of embodiment is stored at room temperature stability
Since tablet will be made derived from the bacterium powder of Examples 1 and 2 in embodiment 4, to being stored at room temperature for the tablet of separate sources
Stability is investigated, as the result is shown (table 3), and lactobacillus bulgaricus bacterium powder still has higher room temperature after mixing with auxiliary material
Hold capacity, product reach storage at normal temperature, transport and the requirement of sale.
3 lactobacillus bulgaricus tablet of table is stored at room temperature stability
Claims (3)
1. a kind of freeze drying protectant for improving lactobacillus bulgaricus and being stored at room temperature ability and anti-pressure ability, which is characterized in that freeze
Do protectant constituent are as follows: pregelatinized starch, skimmed milk powder, maltodextrin and trehalose, surplus are water.
2. freeze drying protectant according to claim 1, which is characterized in that each composition of freeze drying protectant is scored by weight
Not are as follows: 80~120 parts of pregelatinized starch, 50~100 parts of skimmed milk powder, 80~100 parts of maltodextrin, 20~40 parts of trehalose,
640~770 parts of sterile distilled water.
3. a kind of freeze-dried vaccine powder preparation technique for improving lactobacillus bulgaricus and being stored at room temperature ability and anti-pressure ability, feature exist
In including the following steps:
1. after lactobacillus bulgaricus is activated, being amplified fermented and cultured step by step according to the inoculum concentration of 5-10% (v/v), lead to
It crosses centrifugation and collects thallus, bacterium mud and freeze drying protectant are sufficiently mixed according to the weight ratio of 1:1~3, be made in freeze-drying
Machine liquid；
2. upper machine liquid, which will be lyophilized, pours into freeze-drying pallet, upper machine liquid thickness control is put into 0.5~2.0cm at -35 DEG C~-45 DEG C
Pre-freeze 4-8h in freeze dryer；
3. drying process vacuum degree controls between 0.1-0.3mbar always, primary drying process according to 0.5~3 DEG C/h speed
Rate is warming up to 0 DEG C；Parsing-desiccation process is warming up to 30 DEG C from 0 DEG C according to the rate of 1~4 DEG C/h, maintains 2-4h；
4. after vacuum freeze drying, bacterium powder crush to be packed into seal in aluminium plastic bag and deposits in -20 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910482027.1A CN110184191B (en) | 2019-06-04 | 2019-06-04 | Freeze-drying protective agent for improving normal-temperature storage and pressure resistance of lactobacillus bulgaricus and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910482027.1A CN110184191B (en) | 2019-06-04 | 2019-06-04 | Freeze-drying protective agent for improving normal-temperature storage and pressure resistance of lactobacillus bulgaricus and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110184191A true CN110184191A (en) | 2019-08-30 |
| CN110184191B CN110184191B (en) | 2023-02-10 |
Family
ID=67720302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910482027.1A Active CN110184191B (en) | 2019-06-04 | 2019-06-04 | Freeze-drying protective agent for improving normal-temperature storage and pressure resistance of lactobacillus bulgaricus and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110184191B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113621520A (en) * | 2021-08-20 | 2021-11-09 | 微康益生菌(苏州)股份有限公司 | Protective agent for relieving physical extrusion stress damage of probiotic bacteria powder and application thereof |
| CN113755357A (en) * | 2020-06-04 | 2021-12-07 | 内蒙古伊利实业集团股份有限公司 | Lactobacillus preparation and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2799725A1 (en) * | 2009-11-13 | 2010-05-20 | Unistraw Patent Holdings Limited | Probiotic compositions, methods and apparatus for their administration |
| CN102952755A (en) * | 2012-11-19 | 2013-03-06 | 陕西科技大学 | Lactobacillus bulgaricus freeze-drying protecting agent and preparation method thereof |
| CN108851086A (en) * | 2018-06-11 | 2018-11-23 | 西北大学 | One kind is with micropore starch for protectant active lactic acid bacteria microcapsule and preparation method |
-
2019
- 2019-06-04 CN CN201910482027.1A patent/CN110184191B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2799725A1 (en) * | 2009-11-13 | 2010-05-20 | Unistraw Patent Holdings Limited | Probiotic compositions, methods and apparatus for their administration |
| CN102952755A (en) * | 2012-11-19 | 2013-03-06 | 陕西科技大学 | Lactobacillus bulgaricus freeze-drying protecting agent and preparation method thereof |
| CN108851086A (en) * | 2018-06-11 | 2018-11-23 | 西北大学 | One kind is with micropore starch for protectant active lactic acid bacteria microcapsule and preparation method |
Non-Patent Citations (4)
| Title |
|---|
| 周中凯等: "青香蕉淀粉对乳酸菌在冻干和贮存过程中活性的保护效果", 《食品科学》 * |
| 姚卫蓉等: "双歧杆菌活菌粉的制备", 《食品与生物技术》 * |
| 张泽生等: "保加利亚乳杆菌冷冻干燥保护剂的筛选", 《食品工业》 * |
| 白瑛等: "保加利亚乳杆菌B9冷冻保护剂的优化", 《中国奶牛》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755357A (en) * | 2020-06-04 | 2021-12-07 | 内蒙古伊利实业集团股份有限公司 | Lactobacillus preparation and application thereof |
| CN113621520A (en) * | 2021-08-20 | 2021-11-09 | 微康益生菌(苏州)股份有限公司 | Protective agent for relieving physical extrusion stress damage of probiotic bacteria powder and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110184191B (en) | 2023-02-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11202813B2 (en) | Lactobacillus rhamnosus bacterium for treatment of e.g. bacterial vaginosis | |
| CN101724623B (en) | Lactobacillus casei Zhang microcapsule, preparation method and applications thereof | |
| CN108348559B (en) | Lactobacillus paracasei for producing conjugated linoleic acid, nutritional and pharmaceutical preparations containing same, and use thereof | |
| US20040057943A1 (en) | Probiotic strains, a process for the selection of them, compositions thereof, and their use | |
| CN113652359B (en) | Lactic acid bacteria freeze-dried powder, preparation method and freeze-dried protective agent thereof | |
| CN108782758B (en) | Fermented synbiotic goat milk powder and preparation method thereof | |
| CN110607255B (en) | Preparation method and application of lactobacillus delbrueckii and direct vat set lactobacillus delbrueckii starter | |
| CN109929779A (en) | A kind of probiotics preparation and its preparation method and application containing biologically active peptide | |
| CN102763726A (en) | Probiotics yoghourt powder and preparation method thereof | |
| CN112553128B (en) | Probiotic freeze-dried powder and preparation method and application thereof | |
| CN110184191A (en) | A kind of raising lactobacillus bulgaricus is stored at room temperature and the freeze drying protectant of anti-pressure ability and its application | |
| RU2451067C2 (en) | Method for enhancement of bifidobacteria viability in the process of fermented food product storage | |
| CN108018248B (en) | Lactobacillus casei capable of regulating flora structural disorder caused by antibiotics | |
| EP3328208B1 (en) | Methods for the preparation of fermented products comprising bifidobacteria | |
| CN1089567C (en) | Composite microecological health-care food and its preparing process | |
| RU2261909C1 (en) | BIFIDUS-BACTERIUM CONSORTIUM OF Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium infantis, Bifidobacterium breve USEFUL IN PREPARATION OF FERMENTED-MILK AND NONFERMENTED PRODUCTS, BIOACTIVE SUPPLEMENT, BIFIDOBACTERIA- CONTAINING PRODUCT, COSMETIC AND TOILETRY AGENTS | |
| CN107660670A (en) | Bitter buckwheat probiotics drink and preparation method thereof | |
| BG66485B1 (en) | Lactobacillus delbrueckii subsp. bulgaricus strain with a wide range of applications | |
| CN108094531A (en) | A kind of dual probiotics fermented beverage and preparation method thereof | |
| CN103555801A (en) | Method for preparing polypeptide through lactobacillus solid-state fermentation enzymolysis of bean pulps | |
| JP2003527853A (en) | How to supply starter culture with consistent quality | |
| RU2273662C2 (en) | Consortium of microorganisms with probiotic effect | |
| CN102757923B (en) | Method for preparing bifidobacterium bifidum powder with high viable count | |
| CN105815628A (en) | Fermented sesame rich in small peptides and processing method thereof | |
| CN107136217A (en) | Probiotics fermentation-type yoghurt dry powder electuary and its production technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |