CN110183412A - The method of theaflavin is extracted from black tea - Google Patents
The method of theaflavin is extracted from black tea Download PDFInfo
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- CN110183412A CN110183412A CN201910496871.XA CN201910496871A CN110183412A CN 110183412 A CN110183412 A CN 110183412A CN 201910496871 A CN201910496871 A CN 201910496871A CN 110183412 A CN110183412 A CN 110183412A
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- black tea
- theaflavin
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- mixed liquor
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- 244000269722 Thea sinensis Species 0.000 title claims abstract description 47
- IPMYMEWFZKHGAX-UHFFFAOYSA-N Isotheaflavin Natural products OC1CC2=C(O)C=C(O)C=C2OC1C(C1=C2)=CC(O)=C(O)C1=C(O)C(=O)C=C2C1C(O)CC2=C(O)C=C(O)C=C2O1 IPMYMEWFZKHGAX-UHFFFAOYSA-N 0.000 title claims abstract description 44
- UXRMWRBWCAGDQB-UHFFFAOYSA-N Theaflavin Natural products C1=CC(C2C(CC3=C(O)C=C(O)C=C3O2)O)=C(O)C(=O)C2=C1C(C1OC3=CC(O)=CC(O)=C3CC1O)=CC(O)=C2O UXRMWRBWCAGDQB-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 235000014620 theaflavin Nutrition 0.000 title claims abstract description 44
- IPMYMEWFZKHGAX-ZKSIBHASSA-N theaflavin Chemical compound C1=C2C([C@H]3OC4=CC(O)=CC(O)=C4C[C@H]3O)=CC(O)=C(O)C2=C(O)C(=O)C=C1[C@@H]1[C@H](O)CC2=C(O)C=C(O)C=C2O1 IPMYMEWFZKHGAX-ZKSIBHASSA-N 0.000 title claims abstract description 44
- 229940026509 theaflavin Drugs 0.000 title claims abstract description 44
- 235000006468 Thea sinensis Nutrition 0.000 title claims abstract description 42
- 235000020279 black tea Nutrition 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000000605 extraction Methods 0.000 claims abstract description 25
- 238000010992 reflux Methods 0.000 claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 20
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 150000002576 ketones Chemical class 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- BVWCFOXBDSMXEP-UHFFFAOYSA-N 1-(5-acetyl-2-methoxyphenyl)-3-methylbutan-1-one Chemical compound COC1=CC=C(C(C)=O)C=C1C(=O)CC(C)C BVWCFOXBDSMXEP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000006837 decompression Effects 0.000 claims abstract description 7
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 235000013616 tea Nutrition 0.000 claims description 5
- 230000008901 benefit Effects 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 239000004570 mortar (masonry) Substances 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 2
- 238000010025 steaming Methods 0.000 claims 1
- 239000000284 extract Substances 0.000 abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 14
- 238000002835 absorbance Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 238000007254 oxidation reaction Methods 0.000 description 9
- 230000003647 oxidation Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 229960004756 ethanol Drugs 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 229960000935 dehydrated alcohol Drugs 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000012088 reference solution Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000011031 large-scale manufacturing process Methods 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940000406 drug candidate Drugs 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 239000003777 experimental drug Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000007800 oxidant agent Substances 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- -1 1:20 Chemical compound 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- XNLICIUVMPYHGG-UHFFFAOYSA-N methyl n-propyl ketone Natural products CCCC(C)=O XNLICIUVMPYHGG-UHFFFAOYSA-N 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Tea And Coffee (AREA)
Abstract
The method that the present invention provides a kind of from black tea extracts theaflavin, wherein the following steps are included: black tea powder and distilled water are mixed to form mixed liquor, the solid-to-liquid ratio of black tea powder and distilled water is 1:20-1:60；Heating and refluxing extraction is carried out to mixed liquor, reflux temperature is 50 DEG C -100 DEG C, return time 10min-60min, and decompression filters while hot, is cooled to room temperature and obtains filtrate；Different espeleton is added into filtrate or -2 pentanone of 4- methyl is extracted as extractant, obtains the ketone layer containing theaflavin, removal ketone layer obtains theaflavin.
Description
Technical field
The present invention relates to a kind of plant extraction process, in particular to a kind of method that theaflavin is extracted from black tea.
Background technique
An important factor for theaflavin is influence black tea quality, and there is multiple pharmacological effect and health-care efficacy, it is main to wrap
The effects of including anti-oxidant, cancer-resisting, reducing blood lipid, prevention cardiovascular disease, anti-bacteria and anti-virus.With very big potentiality to be exploited,
As people's lives level increasingly improves and environmentally protective theory is rooted in the hearts of the people, people increasingly praise highly green living, tea
The pharmacological function of flavine is also more valued by people, and theaflavin is protected as a kind of natural active constituent in food, medicine
Strong equal fields have broad application prospects, so the extraction of theaflavin and being prepared into research hot topic in recent years.
Currently, the method for extracting theaflavin from black tea mainly has: (1) Collier method: this method is extracted using hot water
Theaflavin is concentrated after removal of impurities, and concentrate is extracted with ethyl acetate, and finally crude product can be obtained in dehydration and drying.This
The advantages of method is that extraction process is simple, the disadvantage is that the amount that organic solvent uses is more, operation is comparatively laborious, and extraction yield
It is less, from an economic point of view, it is unsuitable for large-scale production.(2) Ullah method: this method uses sodium dihydrogen phosphate and acetic acid second
Ester carries out hybrid extraction to extracting solution.Using extracting in boiling water theaflavin, hybrid extraction is carried out after removal of impurities, ester layer after extraction is taken to carry out
It is concentrated and dried, theaflavin crude product can be obtained.The method is easy to operate, as long as carrying out 1 extraction, can be very good to extract simultaneously
Theaflavin is isolated, but the usage amount of organic solvent is still more, extraction efficiency is also not very high.(3) prepared by enzymatic oxidation
Method: promoting the preparation of theaflavin using biological enzyme, and principle is mainly the polyphenol oxidase contained using tealeaves itself.Pass through
Select suitable substrate, and the condition of strict control reaction system, the theaflavin of the extractable more amount out of enzymatic oxidation method；But
Enzymatic oxidation method is more demanding to the selection of substrate and the control of condition, and reaction is complicated, not easy to control.(4) prepared by chemical oxidation
Method: the reaction mechanism that chemical oxidation prepares theaflavin is that theaflavin, chemical oxidation is made using chemical oxidizing agent oxidation catechin
Method can be divided into alkaline oxygenated and acidic oxidation again.Chemical oxidization method can eliminate the restriction of various influence factors, make to react into
Journey is easily controllable, and controllability is stronger, but due to joined chemical oxidizing agent, preparation and purification process relative to direct extraction method compared with
It is complicated.
Summary of the invention
The present invention provides a kind of methods for extracting theaflavin as Extraction solvent using distilled water, can effectively solve
State problem.
The present invention is implemented as follows:
A method of extracting theaflavin from black tea, wherein the following steps are included: black tea powder is mixed with distilled water
The solid-to-liquid ratio of formation mixed liquor, black tea powder and distilled water is 1:20-1:60；Heating and refluxing extraction, reflux are carried out to mixed liquor
Temperature is 50 DEG C -100 DEG C, return time 10min-60min, and decompression filters while hot, is cooled to room temperature and obtains filtrate；To
Different espeleton is added in filtrate or -2 pentanone of 4- methyl is extracted as extractant, obtains the ketone layer containing theaflavin, goes
Except ketone layer obtains theaflavin.
As further improved, the solid-to-liquid ratio (g:mL) of the black tea powder and distilled water is 1:20-1:40.
As further improved, the reflux temperature is 80 DEG C -100 DEG C.
As further improved, the return time is 20min-40min.
The beneficial effects of the present invention are: feasible and environmentally protective as Extraction solvent raw material economics using water；Using directly extraction
Method extracts theaflavin from black tea, and simple process is suitable for large-scale production.
Detailed description of the invention
It, below will be to use required in embodiment in order to illustrate more clearly of the technical solution of embodiment of the present invention
Attached drawing be briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as
It is the restriction to range, it for those of ordinary skill in the art, without creative efforts, can be with root
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the flow chart of the method provided in an embodiment of the present invention that theaflavin is extracted from black tea.
Fig. 2 is the variation schematic diagram of absorbance value provided in an embodiment of the present invention Yu solid-to-liquid ratio parameter.
Fig. 3 is the variation schematic diagram of absorbance value provided in an embodiment of the present invention Yu reflux temperature parameter.
Fig. 4 is the variation schematic diagram of absorbance value provided in an embodiment of the present invention Yu return time parameter.
Specific embodiment
To keep the purposes, technical schemes and advantages of embodiment of the present invention clearer, implement below in conjunction with the present invention
The technical solution in embodiment of the present invention is clearly and completely described in attached drawing in mode, it is clear that described reality
The mode of applying is some embodiments of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ability
Domain those of ordinary skill every other embodiment obtained without creative efforts, belongs to the present invention
The range of protection.Therefore, the detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit below and is wanted
The scope of the present invention of protection is sought, but is merely representative of selected embodiment of the invention.Based on the embodiment in the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts belongs to this
Invent the range of protection.
In the description of the present invention, term " first ", " second " are used for description purposes only, and should not be understood as instruction or dark
Show relative importance or implicitly indicates the quantity of indicated technical characteristic.The feature of " first ", " second " is defined as a result,
It can explicitly or implicitly include one or more of the features.In the description of the present invention, the meaning of " plurality " is two
It is a or more than two, unless otherwise specifically defined.
Shown in referring to Fig.1, the present invention provides a kind of method that theaflavin is extracted from black tea, comprising the following steps:
Black tea powder and distilled water are mixed to form mixed liquor by S1, and the solid-to-liquid ratio of black tea powder and distilled water is 1:20-1:
60；
S2 carries out heating and refluxing extraction to mixed liquor, and reflux temperature is 50 DEG C -100 DEG C, return time 10min-
60min, and decompression filters while hot, is cooled to room temperature and obtains filtrate；
Different espeleton or -2 pentanone of 4- methyl (IBMK) are added into filtrate and is extracted as extractant, obtains by S3
Ketone layer containing theaflavin, removal ketone layer obtain theaflavin.
In step sl, the black tea powder is carried out after removing tea branch with mortar by drying black tea in an oven
Grinding, then the tea-leaf power after grinding is sieved to obtain with sieve.Specifically, the black tea powder is by black tea at 40 DEG C
Baking oven in suitably dry, ground after removing tea branch with mortar, then by the sieve of 20 mesh of the tea-leaf power after grinding into
Row sieving obtains.The solid-to-liquid ratio (g:mL) of the black tea powder and distilled water is 1:20-1:60.Preferably, the black tea powder
Solid-to-liquid ratio (g:mL) with distilled water is 1:20-1:40.In the present embodiment, the solid-to-liquid ratio of the black tea powder and distilled water (g:
It mL) is 1:20.
In step s 2, it when carrying out heating and refluxing extraction to mixed liquor, is heated using thermostat water bath.The reflux
The range of temperature is 50 DEG C -100 DEG C, it is preferable that the range of the reflux temperature is 80 DEG C -100 DEG C.The return time is
10min-60min, it is preferable that the return time is 20min-40min.In the present embodiment, the reflux temperature is 100 DEG C,
The return time is 30min.
In step s3, the method for removing ketone layer is the method for conventional removal ketone layer, is not described in detail herein.
In order to illustrate influence of each preparation parameter to theaflavin content in said extracted method, further using includes to tea
The measuring method of flavine content, comprising the following steps: 0.05mol/L is added in the ketone layer into S3 step containing theaflavin
AlCl3: dehydrated alcohol (1:2) mixed liquor makes its fully reacting after mixing；It is molten for the mixing of 1:2 with ethyl alcohol volume ratio with IBMK
Liquid is reference solution, detects its absorbance with visible spectrophotometer at wavelength 525nm.
The preparation parameter influence extracted in theaflavin method is illustrated below with reference to specific embodiment.Except non-specifically
Illustrate, reagent used in following embodiment and material are commercially available.
Experimental raw: the commercially available black tea in Ningde；Distilled water；Experimental drug is as shown in table 1；Laboratory apparatus is as shown in table 2.
1 experimental drug list of table
| Nomenclature of drug | Purity | Producer |
| Ethyl alcohol | AR | Sinopharm Chemical Reagent Co., Ltd. |
| Aluminum trichloride (anhydrous) | AR | Tianjin Zhi Yuan chemical reagent Co., Ltd |
| - 2 pentanone of 4- methyl | AR | Beijing lark prestige Science and Technology Ltd. |
2 laboratory apparatus list of table
| Instrument title | Model | Producer |
| Visible spectrophotometer | V-1200 | U.S. spectrum in Shanghai reaches Instrument Ltd. |
| Instrument thermostat water bath | Four hole of biserial | Establish instrument and meter Co., Ltd in Shanghai |
| Constant Temp. Oven | DHG-9071A | The upper macro experimental facilities Co., Ltd of Nereid |
| Electronic balance | YP202N | Shanghai Precision Scientific Apparatus Co., Ltd |
| Multiplex vavuum pump of circulating water type | SHZ-D(III) | Zhengzhou Boke experimental instruments and equipment limited |
Embodiment 1
1. providing the solid-to-liquid ratio parameter of five kinds of different black tea powder and distilled water, i.e. 1:20,1:30,1:40,1:50,1:
60。
2. experiment flow: weigh grind after black tea powder 1.000g, be separately added into 20mL, 30mL, 40mL, 50mL,
60mL distilled water, distillation coolant-temperature gage be 80 DEG C, with thermostat water bath at 80 DEG C refluxing extraction 20 minutes, while hot decompression filter,
It is cooled to room temperature；It takes filtrate 5mL in test tube with liquid-transfering gun, IBMK 5mL, concussion layering is added into test tube；It is taken with liquid-transfering gun
0.05mol/L AlCl is added in test tube in the ketone layer solution 2mL on upper layer3: the mixed liquor 6ml of dehydrated alcohol volume ratio 1:2 is mixed
Even postposition is sealed with preservative film, and placing 15min makes its fully reacting；Using the mixed solution of IBMK and ethyl alcohol 1:2 as reference solution,
Absorbance is detected with visible spectrophotometer at wavelength 525nm, testing result joins table 3 and Fig. 2.Figure it is seen that working as
When solid-to-liquid ratio is 1:20, absorbance is maximum.
The influence that theaflavin is extracted when 3 different solid of table is than parameter
Embodiment 2
1. providing six kinds of different reflux temperature parameters, i.e., 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C, 100 DEG C.
2. experiment flow: weighing black tea powder 1.000g, the distilled water that 20mL is added extracts, respectively in 50 DEG C, 60 DEG C, 70
DEG C, 80 DEG C, 90 DEG C, extract at 100 DEG C 20 minutes, decompression filters while hot, is cooled to room temperature；It takes filtrate 5mL in test tube, is added
IBMK 5mL extraction；It takes the ketone layer solution 2mL of extraction in test tube, 0.05mol/L AlCl is added3: dehydrated alcohol volume ratio 1:
2 mixed liquor 6ml makes it react 15min, using the mixed solution of IBMK and ethyl alcohol 1:2 as reference solution, at wavelength 525nm
Its absorbance is detected with visible spectrophotometer, testing result joins table 4 and Fig. 3.From figure 3, it can be seen that when reflux temperature is
At 100 DEG C, absorbance is maximum.
The influence that 4 reflux temperature of table extracts theaflavin
Embodiment 3
1. providing six kinds of different return time parameters, i.e. 10min, 20min, 30min, 40min, 50min, 60min.
2. experiment flow: weighing black tea powder 1.000g, the distilled water that 20mL is added extracts, and extracts respectively at 80 DEG C
10min, 20min, 30min, 40min, 50min, 60min, decompression filters while hot, is cooled to room temperature；Take filtrate 5mL in test tube
In, IBMK 5mL extraction is added；It takes the ketone layer solution 2mL of extraction in test tube, 0.05mol/L AlCl is added3: dehydrated alcohol
The mixed liquor 6ml of volume ratio 1:2 makes it react 15min；Using the mixed solution of IBMK and ethyl alcohol 1:2 as reference solution, in wavelength
Its absorbance is detected with visible spectrophotometer at 525nm, testing result joins table 5 and Fig. 4.From fig. 4, it can be seen that when reflux
When time is 30 minutes, absorbance is maximum.
The influence that 5 return time of table extracts theaflavin
Further, when extracting theaflavin, select solid-to-liquid ratio parameter for 1:20, reflux temperature is 100 DEG C, and return time is
When 30min, the absorbance detected is 0.167.
The method provided by the invention for extracting theaflavin from black tea has the advantage that using direct extraction method from black tea
Theaflavin is extracted, simple process is suitable for large-scale production；It is feasible and environmentally protective as Extraction solvent raw material economics using water.
The foregoing is merely the preferred embodiment of the present invention, are not intended to restrict the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of method for extracting theaflavin from black tea, which comprises the following steps:
Black tea powder and distilled water are mixed to form mixed liquor, the solid-to-liquid ratio of black tea powder and distilled water is 1:20-1:60；
Heating and refluxing extraction is carried out to mixed liquor, reflux temperature is 50 DEG C -100 DEG C, return time 10min-60min, and is taken advantage of
Heat decompression filters, and is cooled to room temperature and obtains filtrate；
Different espeleton is added into filtrate or -2 pentanone of 4- methyl is extracted as extractant, obtains the ketone containing theaflavin
Layer, removal ketone layer obtain theaflavin.
2. the method according to claim 1 for extracting theaflavin from black tea, which is characterized in that the black tea powder is logical
It crosses and dries black tea in an oven, ground after removing tea branch with mortar, then the tea-leaf power after grinding is carried out with sieve
Sieving obtains.
3. the method according to claim 1 for extracting theaflavin from black tea, which is characterized in that the black tea powder and steaming
The solid-to-liquid ratio (g:mL) of distilled water is 1:20-1:40.
4. the method according to claim 1 for extracting theaflavin from black tea, which is characterized in that heated to mixed liquor
It is to be heated using thermostat water bath.
5. the method according to claim 1 for extracting theaflavin from black tea, which is characterized in that the reflux temperature is 80
℃-100℃。
6. the method according to claim 1 for extracting theaflavin from black tea, which is characterized in that the return time is
20min-40min。
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| CN111732566A (en) * | 2020-08-04 | 2020-10-02 | 大湾汉唯(广州)医药科技集团有限公司 | Method for extracting theaflavin |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111732566A (en) * | 2020-08-04 | 2020-10-02 | 大湾汉唯(广州)医药科技集团有限公司 | Method for extracting theaflavin |
| CN111732566B (en) * | 2020-08-04 | 2020-11-27 | 大湾汉唯(广州)医药科技集团有限公司 | Method for extracting theaflavin |
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