CN110180612A - A kind of micro-fluidic chip and its miniflow driving control system - Google Patents
A kind of micro-fluidic chip and its miniflow driving control system Download PDFInfo
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- CN110180612A CN110180612A CN201910668375.8A CN201910668375A CN110180612A CN 110180612 A CN110180612 A CN 110180612A CN 201910668375 A CN201910668375 A CN 201910668375A CN 110180612 A CN110180612 A CN 110180612A
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- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 24
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000012360 testing method Methods 0.000 claims abstract description 16
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The present invention provides a kind of micro-fluidic chip and its miniflow driving control systems, belong to field of micro electromechanical technology, the chip includes control layer, PDMS film layer, fluid channel layer and substrate of glass, fluid channel layer is provided with 1 main channel and 3 sorting channels, the switch for sorting channel is controlled by the thermal expansion micro-valve door of control layer, the miniflow driving control system includes observation device and fluid control apparatus, microfluidic system integrality proposed by the present invention solves miniflow focusing, the technical problems such as miniflow driving and miniflow detection, the separation system of design can realize automatic clustering for different testing sample, the fluid control apparatus of design can substitute existing multiple miniflow transfer tubes, the program advances the scientific research of micro-fluidic chip and miniflow actuation techniques.
Description
Technical field
The invention belongs to bio-microelectromechanical technical fields, and in particular to a kind of micro-fluidic chip and its miniflow drive control system
System.
Background technique
Micro-fluidic is one kind accurately control and manipulation minute yardstick fluid technique, micro-fluidic using for fluid under minute yardstick
It includes engineering science, physics, chemistry, more cross disciplines of micro Process and bioengineering that control, which is one,.Micro-fluidic chip is made
Belong to bio-microelectromechanical technical field important technology branch for the core tool of microflow control technique, micro-fluidic detection chip has sample
The advantages that product consume less, detection speed is fast, easy to operate, multifunctional unit, small and easy to carry body, can be widely used in
The technical fields such as biology, medicine, chemical industry, in the prior art, the structure and function of micro-fluidic chip are single, do not have automatic inspection
The functions such as survey, sorting cause application scenarios limited, its development are constrained, in particular, miniflow driving control system mostly uses foreign countries
Import equipment lacks the miniflow drive system of independent research.
Summary of the invention
In view of this, microfluidic system integrality proposed by the present invention solves miniflow focusing, miniflow driving and miniflow
The separation system of the technical problems such as detection, design can realize automatic clustering for different testing sample, and the liquid stream of design controls dress
Existing multiple miniflow transfer tubes can be substituted by setting, and the program advances the scientific research of micro-fluidic chip and miniflow actuation techniques.
In order to achieve the object of the present invention, micro-fluidic chip of the invention adopts the following technical scheme that
A kind of micro-fluidic chip includes control layer, PDMS film layer, fluid channel layer and substrate of glass, in which: the control layer
Multiple micro-valve doors for open and close fluid channel are inside provided with, the micro-valve door is thermal expansion valve；In the fluid channel layer
Be provided with 1 main channel, the input terminal of main channel is connected to sample cell, be provided with observation section in the middle part of main channel, main channel it is defeated
Outlet is separately connected three sorting channels, is provided with the micro-valve door above the sorting channel；In the input terminal and sight
It surveys between section to be symmetrically arranged with and focuses sheath stream group；Driving sheath stream group is symmetrically arranged between observation section and sorting channel；It is described
Sheath stream group is focused for controlling detectable substance single-row layout and movement and including the first sheath circulation road and the second sheath circulation road, the
Under the promotion of one sheath circulation road and the second sheath circulation road, detectable substance can sequentially enter observation section；The driving sheath stream group is used for
Driving detectable substance advances and including third sheath circulation road and the 4th sheath circulation road, in third sheath circulation road and the 4th sheath circulation road
Promotion under, detectable substance is according to being set into different sorting channels；The sorting channel includes the first sorting channel, second point
It gates road and third sorts channel, first sorting channel, the second sorting channel and third sorting channel are respectively communicated with difference
Sorting pool；The micro-valve door includes the first micro-valve door, the second micro-valve door and third micro-valve door, in which: the first micro-valve door
The top in the first sorting channel is set, and the diminution or expansion of the first micro-valve door can drive the deformation of PDMS film layer to realize the
The on and off in one sorting channel, the diminution or expansion of the second micro-valve door can drive the deformation of PDMS film layer to realize second
The on and off in channel is sorted, the diminution or expansion of third micro-valve door can drive the deformation of PDMS film layer to realize third point
Gate the on and off in road；The sheath circulation road and the angle of main channel are 72 °, and the two sides of the sheath circulation road import bevel edge
Angle with main channel is 16 °.
Preferably, the micro-valve door is internally provided with heating sheet, and the heating sheet is made of alloy platinum material, and heating sheet uses
The mutually double helix shaped form structure of coiling and noninterlace, the outer profile width of the double helix shaped form structure are 700 μm.
Preferably, the valve chamber of the thermal expansion valve uses circular configuration, and the radius of round valve chamber is 850 μm, close to institute
State the PDMS film layer of round valve chamber with a thickness of 120 μm.
Preferably, the control layer is glass material, and the valve chamber is provided on the control layer by photoetching process, institute
Heating sheet is stated to be arranged by sputtering technology in valve chamber bottom.
A kind of micro-fluidic chip of the invention has the advantages that
(1) micro-fluidic chip uses novel focusing angle and designs with one voice, and sheath circulation road and main channel exist centainly
Angle, meanwhile, there are a buffering bevel edge when sheath circulation road imports main channel, the transition position of sheath circulation road is round-corner transition,
This design scheme can effectively promote focusing effect, realize the well focussed under low speed, focus sheath stream group in the present invention program
In flow rate of liquid be only that preferable focusing effect can be realized in 8-12m/s.
(2) include 3 sorting channels and corresponding micro-valve door in the present invention, closed by controlling different micro-valve doors
Or open the classification and sorting for realizing different testing sample.
(3) the solution of the present invention can be combined with image capturing system and Computerized analysis system, based on detectable substance
Image and video, realization are automatically analyzed and are sorted.
(4) present invention is using newly-designed thermal expansion valve, the diameter of valve chamber, elastic PDMS film layer thickness,
Material, geometric layout of heating sheet of heating sheet etc., which have all done, targetedly to be designed and optimizes, compared with Air control valve, on the one hand
It no longer needs air pump and gas circuit to can reduce the volume of chip, on the other hand can reduce valve control difficulty, it is only necessary to control
Conduction time and power-on voltage, under the driving of 10-20V voltage, the delay time of the thermal expansion valve only has 1.5-2s.
A kind of miniflow driving control system for above-mentioned micro-fluidic chip includes observation device and fluid control apparatus,
In:
The observation device, which is divided into, tests and analyzes device and sorting observation device, and observation section is arranged in the device that tests and analyzes
Side, tests and analyzes physical aspect or spectral information that device is used to obtain detectable substance, and the sorting observation device is arranged defeated
The side of outlet, sorting observation device are used to feed back the screening motion profile of detectable substance, and the physical aspect includes detectable substance
Size, profile and transparence information, the spectral information include Raman spectrum or fluorescence information；
The fluid control apparatus includes cabinet, sample plunger pump, the first sheath stream plunger pump, the second sheath stream plunger pump and transmission
Group, in which: the box house is provided with power supply and control panel, motor, fixing seat, the multiple fixing bearings for installing motor
The electromagnet and reset that seat, two groups of plain bearing housings, the sliding slot for installing plain bearing housing and driving plain bearing housing move
Spring；The sample plunger pump includes that sample cell, sample push rod, sample chock plug, sample rack gear, sample inlet tube and sample go out liquid
Pipe, for receiving sample, one end of the sample push rod is moveably mounted on cabinet the sample cell, sample push rod it is another
One end is mounted in sample cell, and the sample chock plug is used for sealed sample pipe and is mounted on the other end of sample push rod, the sample
Product chock plug and sample inside pipe wall form sample cavity, and the sample inlet tube and sample outlet tube are respectively communicated with sample cavity, sample into
Liquid pipe is connected to sample cell by hose, and sample outlet tube is connected to main channel by hose, and the sample rack gear setting is pushed away in sample
On bar, sample rack gear is driven by transmission group；The first sheath stream plunger pump includes the first sheath flow tube, the first sheath stream push rod, first
Sheath stream chock plug, the first sheath stream rack gear, the first sheath stream inlet tube and the first sheath stream outlet tube, the first sheath stream pipe is for receiving sheath
One end of flow liquid, the first sheath stream push rod is moveably mounted on cabinet, and the other end of the first sheath stream push rod is mounted on
In one sheath flow tube, the first sheath stream chock plug is used to seal the first sheath flow tube and is mounted on the other end of the first sheath stream push rod, institute
It states the first sheath stream chock plug and the first sheath stream inside pipe wall forms the first sheath stream chamber, the first sheath stream inlet tube and the first sheath efflux
Pipe is respectively communicated with the first sheath stream chamber, and the first sheath stream inlet tube is connected to sheath liquid pool, and the first sheath stream outlet tube is divided into two by hose
It is connected to the first sheath circulation road and the second sheath circulation road, the first sheath stream rack gear is arranged on the first sheath stream push rod, the first sheath stream
Rack gear is by transmission group (driving；The second sheath stream plunger pump include the second sheath flow tube, the second sheath stream push rod, the second sheath stream chock plug,
Second sheath stream rack gear, the second sheath stream inlet tube and the second sheath stream outlet tube, the second sheath stream pipe are described for receiving sheath flow liquid
One end of second sheath stream push rod is moveably mounted on cabinet, and the other end of the second sheath stream push rod is mounted on the second sheath flow tube
Interior, the second sheath stream chock plug is used to seal the second sheath flow tube and is mounted on the other end of the second sheath stream push rod, second sheath
It flows chock plug and the second sheath stream inside pipe wall forms the second sheath stream chamber, the second sheath stream inlet tube and the second sheath stream outlet tube connect respectively
Logical second sheath stream chamber, the second sheath stream inlet tube are connected to sheath liquid pool, and the second sheath stream outlet tube is divided into two by hose is connected to third
Sheath circulation road and the 4th sheath circulation road；The sample inlet tube, sample outlet tube, the first sheath stream inlet tube, the first sheath efflux
Manually-operated gate or solenoid valve are respectively arranged on pipe, the second sheath stream inlet tube and the second sheath stream outlet tube；The transmission group is used for
Drive the first sheath stream plunger pump, the second sheath stream plunger pump and sample plunger pump work.
Preferably, the transmission group includes motor gear, sample reduction gearing, sheath stream reduction gearing, the transmission of the first sheath stream
Cone pulley, the first sheath clutch cone pulley, the first sheath stream driving cone pulley, the first sheath stream drive gear, the second sheath stream drive conical wheel, the second sheath
Clutch cone pulley, the second sheath stream driving cone pulley and the second sheath stream drive gear, in which: the motor gear is set in machine shaft
On, the side gearing motor gear of the sample reduction gearing；Engage sample rack gear, the sheath stream in the side of sample reduction gearing
Reduction gearing, the first sheath stream drive conical wheel and the second sheath stream drive conical wheel are coaxially arranged, sheath stream reduction gearing gearing motor gear,
First sheath stream drive conical wheel and the second sheath stream drive conical wheel are arranged in the two sides of sheath stream reduction gearing；The first sheath clutch cone pulley
It is mounted on plain bearing housing, the first sheath stream driving cone pulley is mounted on the first sheath clutch cone pulley side, is slided by mobile
Bearing block can be realized the engagement between the first sheath stream drive conical wheel, the first sheath clutch cone pulley and the first sheath stream driving cone pulley or divide
From first sheath stream driving gear and the driving cone pulley coaxial arrangement of the first sheath stream, the first sheath stream driving gear engage the
One sheath stream rack gear；The second sheath clutch cone pulley is mounted on plain bearing housing, and second sheath stream driving cone pulley is mounted on the
Two sheath clutch cone pulley sides, by mobile plain bearing housing can be realized the second sheath stream drive conical wheel, the second sheath clutch cone pulley and
Second sheath stream drives the engagement or separation between cone pulley, and the second sheath stream driving gear is coaxially set with the second sheath stream driving cone pulley
It sets, the second sheath stream driving gear engages the second sheath stream rack gear.
Preferably, the top and the transmission of the second sheath stream of the first sheath stream drive conical wheel and the first sheath stream driving cone pulley
The bottom of cone pulley and the second sheath stream driving cone pulley offers connection dimpled grain respectively, the bottom of the first sheath clutch cone pulley and the
The top of two sheath clutch cone pulleys offers corresponding connection burr respectively.
Preferably, the first sheath stream drive conical wheel, the first sheath stream drive cone pulley, the first sheath clutch cone pulley, the second sheath stream
Drive conical wheel, the second sheath stream driving cone pulley and the second sheath clutch cone pulley are step cone pulley, and the step cone pulley includes middle part
The inner cone and rear cone at table top and both ends are connected, is provided with anti-skid chequer on the connection table top.
Preferably, the reset spring is pressure spring, and when electromagnet is not powered on, reset spring drives the first sheath clutch cone pulley
Or second sheath clutch cone pulley far from drive conical wheel group, when the solenoid is energized, electromagnet generate magnetic field and be arranged in sliding axle
The identical generation repulsion of permanent magnet field for holding seat pushes the first sheath clutch cone pulley or the second sheath clutch cone pulley engaged transmission cone pulley group.
Preferably, the reset spring is tension spring, and when electromagnet is not powered on, reset spring drives the first sheath clutch cone pulley
Or the second sheath clutch cone pulley engaged transmission cone pulley group, when the solenoid is energized, the magnetic field and be arranged in sliding axle that electromagnet generates
The permanent magnet field for holding seat generates suction on the contrary and pushes the first sheath clutch cone pulley or the second sheath clutch cone pulley far from drive conical wheel group.
Miniflow driving control system of the invention has the advantages that
(1) sample plunger pump, the first sheath stream plunger pump and the second sheath fluidization tower can be realized using a motor in fluid control apparatus
The driving of pump is filled in, in particular, can be realized the timesharing driving of the first sheath stream plunger pump and the second sheath stream plunger pump, in different periods
It realizes and focuses sheath stream group or driving sheath stream group work.
(2) sample plunger pump, the first sheath stream plunger pump and the second sheath stream may be implemented by the way that different reduction gearing is arranged
The different driving velocities of plunger pump, simultaneously as can be realized while starting using gear connection scheme, be simultaneously stopped.
(3) the gear clutch scheme designed can satisfy the transmission requirement of low-power, and structure is simple, low manufacture cost, should
Magnetic force moving can be used in gear clutch system, and magnetic force has elasticity, under the action of elastic force and non-slip texture, may be implemented
The engagement and Synchronous Transmission of gear.
(4) by conjunction with observation device, realizing the analysis and classification of object to be detected, observation device may include this method
One or more of CCD, video camera, Raman spectrum detecting device or fluorescence detection device, image and view based on detectable substance
Frequently, it is detected automatically by computer, signal collection, data processing, after analysis result judgement goes out structure, computer control
Micro- valve opening and closing is made, to make it into corresponding pipeline, realizes sorting.
Detailed description of the invention
Fig. 1 is the fluid channel schematic diagram of a layer structure of micro-fluidic chip in the present invention；
Fig. 2 is the on state schematic diagram of PDMS thermal control micro-valve in the present invention；
Fig. 3 is the closing structure schematic diagram of PDMS thermal control micro-valve in the present invention；
Fig. 4 is the heating sheet structural schematic diagram of PDMS thermal control micro-valve in the present invention；
Fig. 5 is the plunger pump structure schematic diagram of fluid control apparatus in the present invention；
Fig. 6 is the transmission group structural schematic diagram of fluid control apparatus in the present invention；
Fig. 7 is clutch cone pulley operation schematic diagram in the present invention；
Fig. 8 is the spread groove schematic diagram of transmission clutch in the present invention；
Fig. 9 is step cone pulley structural schematic diagram in the present invention.
In figure, 1- micro-fluidic chip, 101- control layer, the first micro-valve of 1011- door, the second micro-valve of 1012- door, 1013-
Three micro-valve doors, 102-PDMS film layer, 103- fluid channel layer, the main channel 1031-, 10311- input terminal, 10312- observation section,
10313- output end, the first sheath of 1032- circulation road, the second sheath of 1033- circulation road, 1034- third sheath circulation road, 1035- the 4th
Sheath circulation road, 1036- first sort channel, 1037- second sort channel, 1038- third sorting channel, 104- substrate of glass,
2- observation device, 201- test and analyze device, 202- sorting observation device, 3- heating sheet, 4- fluid control apparatus, 41- cabinet,
42- sample plunger pump, the first sheath stream of 43- plunger pump, the second sheath stream of 44- plunger pump, 45- transmission group, 411- power supply and control panel,
412- motor, 413- fixing seat, 414- fixed bearing block, 415- plain bearing housing, 416- sliding slot, 417- electromagnet, 418- are multiple
Position spring, 421- sample cell, 422- sample push rod, 423- sample chock plug, 424- sample rack gear, 425- sample inlet tube, 426-
Sample outlet tube, the first sheath of 431- flow tube, the first sheath stream of 432- push rod, the first sheath stream of 433- chock plug, the first sheath stream of 434- rack gear,
The first sheath stream of 435- inlet tube, the first sheath stream of 436- outlet tube, the second sheath of 441- flow tube, the second sheath stream of 442- push rod, 443-
Two sheath stream chock plugs, the second sheath stream of 444- rack gear, the second sheath stream of 445- inlet tube, the second sheath stream of 446- outlet tube, 451- motor tooth
Wheel, 452- sample reduction gearing, 453- sheath stream reduction gearing, the first sheath stream of 454- drive conical wheel, 455- the first sheath clutch cone pulley,
The first sheath stream of 456- drives cone pulley, the first sheath stream of 457- to drive gear, the second sheath stream of 458- drive conical wheel, 459- the second sheath clutch
Cone pulley, the second sheath stream of 4510- driving cone pulley, the second sheath stream of 4511- driving gear, 4512- connection dimpled grain, 4513- connection burr,
4514- connection table top, 4515- inner cone, 4516- rear cone.
Specific embodiment
In the description of the present invention, it is to be understood that, term " center ", "upper", "lower", "front", "rear", " left side ",
The orientation or positional relationship of the instructions such as " right side ", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on the figure
Orientation or positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device of indication or suggestion meaning or
Element must have a particular orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
Term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance or implicitly indicate
The quantity of indicated technical characteristic." first " is defined as a result, the feature of " second " can explicitly or implicitly include one
A or more this feature.In the description of the present invention, unless otherwise indicated, the meaning of " plurality " is two or two with
On.
As shown in Figure 1, micro-fluidic chip 1 includes control layer 101, PDMS film layer 102, fluid channel layer 103 and glass
Substrate 104, wherein PDMS is the abbreviation of polydimethylsiloxane, and Chinese is dimethyl silicone polymer, which is to have
One kind of machine silicon using simple, with having good adhesiveness between silicon wafer, and has good chemistry because its is at low cost
The features such as inertia, becomes a kind of polymer material for being widely used in micro-fluidic equal fields, control of the micro-fluidic chip 1 in computer
Can be realized under system detectable substance it is automatic detection, focus, advance, it is static, sorting etc. functions.
When practical operation, control layer 101, PDMS film layer 102, fluid channel layer 103 and substrate of glass 104 are by existing
There is glass-engraving technology to combine with polymer transfer adhering technique to be made.
In Fig. 1,3 micro-valve doors for open and close fluid channel are provided in control layer 101；It is set in fluid channel layer 103
It is equipped with main channel 1031, the input terminal 10311 of main channel 1031 is connected to sample cell, and the middle part of main channel 1031 is provided with observation section
10312, the output end 10313 of main channel 1031 is separately connected 3 sorting channels, sorts and is provided with micro-valve door above channel；
It is symmetrically arranged between input terminal 10311 and observation section 10312 and focuses sheath stream group；Observation section 10312 and sorting channel it
Between be symmetrically arranged with driving sheath stream group.
It should be noted that focusing sheath stream group for controlling detectable substance single-row layout and movement and circulating including the first sheath
Road 1032 and the second sheath circulation road 1033, under the promotion of the first sheath circulation road 1032 and the second sheath circulation road 1033, detectable substance
Observation section 10312 can be sequentially entered；Driving sheath stream group is for driving detectable substance to advance and including 1034 and of third sheath circulation road
4th sheath circulation road 1035, under the promotion of third sheath circulation road 1034 and the 4th sheath circulation road 1035, detectable substance is according to setting
Into different sorting channels.
Specifically, sorting channel in Fig. 1 includes that the first sorting channel 1036, second sorting channel 1037 and third sorting are logical
Road 1038, the first sorting channel 1036, second sort channel 1037 and third sorting channel 1038 and are respectively communicated with different sortings
Pond.
It should be noted that the angular range of the sheath circulation road of the program and main channel 1031 is 72 °, the remittance of sheath circulation road
The angle for entering bevel edge and main channel is 16 °, focuses the flow rate of liquid in sheath stream group and is set as 8-12m/s, drives in sheath stream group
Flow rate of liquid is set as 6-10m/s.
Specifically, the quantity in micro-valve door and sorting channel can be with flexible setting.
Micro-valve door includes the first micro-valve door 1011, the second micro-valve door 1012 and third micro-valve door 1013, in which: the first micro-valve
The top in the first sorting channel 1036 is arranged in door 1011, and the diminution or expansion of the first micro-valve door 1011 can drive PDMS film
To realize the on and off in the first sorting channel 1036, the diminution or expansion of the second micro-valve door 1012 can drive 102 deformation of layer
Dynamic 102 deformation of PDMS film layer to realize the on and off in the second sorting channel 1037, the diminution of third micro-valve door 1013 or
Expansion can drive 102 deformation of PDMS film layer to realize the on and off in third sorting channel 1038.
As shown in Figures 2 and 3, film micro-valve structure is a kind of common micro-valve door, uses dimethyl silicone polymer system
Make, specifically divide upper, middle and lower three-decker: upper layer is control layer 101, and middle layer is PDMS film layer 102, and lower layer is that fluid flowing is logical
Road.Above-mentioned micro-valve door is thermal expansion valve, and the principle of thermal expansion type micro-valve is, by the metal heater for being placed in inside cavity
Heating expands closed cavity, to generate the upward deformation of elastic membrane, and then blocking channel, completes the logical of fluid channel
Disconnected control.
It should be noted that micro-valve door is internally provided with heating sheet 3, heating sheet 3 is made of alloy platinum material, and heating sheet 3 is adopted
With mutual coiling and the double helix shaped form structure of noninterlace, the outer profile width of double helix shaped form structure is 700 μm, is such as schemed
Shown in 4.The valve chamber for thermally expanding valve uses circular configuration, and the radius of round valve chamber is 850 μm, and the PDMS close to round valve chamber is thin
Film layer 102 with a thickness of 120 μm.Control layer 101 is glass material, and valve chamber is provided on control layer 101 by photoetching process, is added
Backing 3 is arranged by sputtering technology in valve chamber bottom.
Specifically, process valve chamber in glass surface using photoetching process, first in clean glass surface spin coating photoresist,
After photoetching, the Cr of 15nm is sputtered as adhesion layer, the platinum of 80nm is then sputtered as heater, the glass sputtered is placed in third
Ultrasound demoulding, obtains heater in ketone solution.
It further include observation device 2 and fluid control apparatus 4 to realize the automatic control of chip in the present embodiment,
In: in Fig. 2, observation device 2 divides to test and analyze device 201 and sorting observation device 202, tests and analyzes the setting of device 201 and exists
The side of section 10312 is observed, physical aspect or spectral information that device 201 is used to obtain detectable substance, sorting observation are tested and analyzed
The side of output end 10313 is arranged in device 202, and sorting observation device 202 is used to feed back the screening motion profile of detectable substance, object
Reason form includes size, profile and the transparence information of detectable substance, and spectral information includes Raman spectrum or fluorescence information.
Specifically, testing and analyzing device 201 can be high definition CCD, high-definition camera, Raman spectrum detecting device or fluorescence
One of detection device, sorting observation device 202 can be high definition CCD or high-definition camera.
As shown in Fig. 5, Fig. 6 and Fig. 7, fluid control apparatus 4 includes cabinet 41, sample plunger pump 42, the first sheath stream plunger
Pump the 43, second sheath stream plunger pump 44 and transmission group 45, in which:
Cabinet 41 is internally provided with power supply and control panel 411, motor 412, the fixing seat 413 for installing motor 412, multiple fixations
414, two groups of plain bearing housings 415 of bearing block, the sliding slot 416 for installing plain bearing housing 415 and driving plain bearing housing
The electromagnet 417 and reset spring 418 of 415 movements, power supply and control panel 411 are used to power for motor 412, while for controlling
Plain bearing housing 415 works, and power supply and control panel 411 can directly be connect with host computer, and power supply and control panel 411 can also be set
It is equipped with outer button, receives manually controlling for user, two groups of plain bearing housings 415 are mounted in sliding slot 416,417 He of electromagnet
Reset spring 418 can control the position of plain bearing housing 415, specific as follows:
Situation one: reset spring 418 is tension spring, and when electromagnet 417 is not powered on, reset spring 418 drives the first sheath clutch cone
459 engaged transmission cone pulley group of wheel 455 or the second sheath clutch cone pulley, when electromagnet 417 is powered, the magnetic field of the generation of electromagnet 417
It is opposite with the permanent magnet field that plain bearing housing 415 is arranged in generate suction push the first sheath clutch cone pulley 455 or the second sheath from
Cone pulley 459 is closed far from drive conical wheel group.
Situation two: reset spring 418 be pressure spring, when electromagnet 417 is not powered on, reset spring 418 drive the first sheath from
Cone pulley 455 or the second sheath clutch cone pulley 459 are closed far from drive conical wheel group, when electromagnet 417 is powered, what electromagnet 417 generated
Magnetic field generation repulsion identical as the permanent magnet field that plain bearing housing 415 is arranged in pushes the first sheath clutch cone pulley 455 or the second
459 engaged transmission cone pulley group of sheath clutch cone pulley.
In figure, sample plunger pump 42 includes sample cell 421, sample push rod 422, sample chock plug 423, sample rack gear 424, sample
Product inlet tube 425 and sample outlet tube 426, sample cell 421 for receiving sample, movably pacify by one end of sample push rod 422
On cabinet 41, the other end of sample push rod 422 is mounted in sample cell 421, and sample chock plug 423 is used for sealed sample pipe
421 and it is mounted on the other end of sample push rod 422, sample chock plug 423 and 421 inner wall of sample cell form sample cavity, sample feed liquor
Pipe 425 and sample outlet tube 426 are respectively communicated with sample cavity, and sample inlet tube 425 is connected to sample cell, sample outlet tube by hose
426 are connected to main channel 1031 by hose, and sample rack gear 424 is arranged on sample push rod 422, and sample rack gear 424 is by transmission group
45 drivings, the first sheath stream plunger pump 43 include the first sheath flow tube 431, the first sheath stream push rod 432, the first sheath stream chock plug 433, first
Sheath stream rack gear 434, the first sheath stream inlet tube 435 and the first sheath stream outlet tube 436, the first sheath flow tube 431 are used to receive sheath flow liquid,
One end of first sheath stream push rod 432 is moveably mounted on cabinet 41, and the other end of the first sheath stream push rod 432 is mounted on first
In sheath flow tube 431, the first sheath stream chock plug 433 is for sealing the first sheath flow tube 431 and being mounted on the another of the first sheath stream push rod 432
End, the first sheath stream chock plug 433 and 431 inner wall of the first sheath flow tube form the first sheath stream chamber, the first sheath stream inlet tube 435 and the first sheath
Outflow liquid pipe 436 is respectively communicated with the first sheath stream chamber, and the first sheath stream inlet tube 435 is connected to sheath liquid pool, and the first sheath stream outlet tube 436 is logical
It crosses hose to be divided into two connection the first sheath circulation road 1032 and the second sheath circulation road 1033, the setting of the first sheath stream rack gear 434 is the
On one sheath stream push rod 432, the first sheath stream rack gear 434 is driven by transmission group 45；Second sheath stream plunger pump 44 includes the second sheath flow tube
441, the second sheath stream push rod 442, the second sheath stream chock plug 443, the second sheath stream rack gear 444, the second sheath stream inlet tube 445 and the second sheath
Liquid pipe 446 is flowed out, for the second sheath flow tube 441 for receiving sheath flow liquid, one end of the second sheath stream push rod 442 is moveably mounted to case
On body 41, the other end of the second sheath stream push rod 442 is mounted in the second sheath flow tube 441, and the second sheath stream chock plug 443 is for sealing the
Two sheath flow tubes 441 and the other end for being mounted on the second sheath stream push rod 442, the second sheath stream chock plug 443 and 441 inner wall of the second sheath flow tube
The second sheath stream chamber is formed, the second sheath stream inlet tube 445 and the second sheath stream outlet tube 446 are respectively communicated with the second sheath stream chamber, the second sheath
It flows into liquid pipe 445 and is connected to sheath liquid pool, the second sheath stream outlet tube 446 is divided into two by hose is connected to 1034 He of third sheath circulation road
4th sheath circulation road 1035；Sample inlet tube 425, sample outlet tube 426, the first sheath stream inlet tube 435, the first sheath stream outlet tube
436, manually-operated gate or solenoid valve are respectively arranged on the second sheath stream inlet tube 445 and the second sheath stream outlet tube 446.
Specifically, being provided with 3 through-holes on cabinet 41, sliding bearing is installed on through-hole to guarantee sample push rod 422,
One sheath stream push rod 432, the second sheath stream push rod 442 are flexibly mobile.
When specific works, it is only necessary to control the positive and negative rotation of motor 412 and the on-off of solenoid valve, sample plunger can be realized
The 42, first sheath stream plunger pump 43, the automatic imbibition of the second sheath stream plunger pump 44 and propulsive liquid body are pumped, is truly realized on automatic
Liquid.
In figure, transmission group 45 is for driving the first sheath stream plunger pump 43, the second sheath stream plunger pump 44 and sample plunger pump 42
Work, specifically, transmission group 45 includes motor gear 451, sample reduction gearing 452, sheath stream reduction gearing 453, the first sheath stream
Drive conical wheel 454, the first sheath clutch cone pulley 455, the first sheath stream driving cone pulley 456, the first sheath stream drive gear 457, the second sheath
It flows drive conical wheel 458, the second sheath clutch cone pulley 459, the second sheath stream driving cone pulley 4510 and the second sheath stream and drives gear 4511,
In: motor gear 451 is set in 412 shaft of motor, the side gearing motor gear 451 of sample reduction gearing 452；Sample subtracts
Engage sample rack gear 424, sheath stream reduction gearing 453, the first sheath stream drive conical wheel 454 and the second sheath stream in the side of fast gear 452
Drive conical wheel 458 is coaxially arranged, 453 gearing motor gear 451 of sheath stream reduction gearing, the first sheath stream drive conical wheel 454 and second
Sheath stream drive conical wheel 458 is arranged in the two sides of sheath stream reduction gearing 453；First sheath clutch cone pulley 455 is mounted on plain bearing housing
On 415, the first sheath stream driving cone pulley 456 is mounted on 455 side of the first sheath clutch cone pulley, passes through mobile 415 energy of plain bearing housing
Enough realize the first sheath stream drive conical wheel 454, the first sheath clutch cone pulley 455 and the first sheath stream driving cone pulley 456 between engagement or
Separation, the first sheath stream drive gear 457 and the first sheath stream driving cone pulley 456 to be coaxially disposed, and the first sheath stream drives gear 457 to engage
First sheath stream rack gear 434；Second sheath clutch cone pulley 459 is mounted on plain bearing housing 415, and the second sheath stream drives cone pulley 4510 to pacify
Mounted in 459 side of the second sheath clutch cone pulley, the second sheath stream drive conical wheel 458, the can be realized by mobile plain bearing housing 415
Two sheath clutch cone pulleys 459 and the second sheath stream driving cone pulley 4510 between engagement or separation, the second sheath stream drive gear 4511 with
Second sheath stream drives cone pulley 4510 to be coaxially disposed, and the second sheath stream drives gear 4511 to engage the second sheath stream rack gear 434.
When specific work, the output torque of motor 412 is divided into two by motor gear 451, and a part passes to sample
Product reduction gearing 452, another part pass to sheath stream reduction gearing 453, and sample reduction gearing 452 passes through 424 band of sample rack gear
Dynamic sample push rod 422 is mobile to realize that sample plunger pump 42 works；Sheath stream reduction gearing 453 drives the first sheath of coaxial arrangement to spread
Mantle wheel 454 and the second sheath stream drive conical wheel 458, if the first sheath clutch cone pulley 455 and the second sheath clutch cone pulley 459 are not situated between
Enter transmission group, the first sheath stream drive conical wheel 454 and the second sheath stream drive conical wheel 458 are dallying, when the first sheath clutch cone pulley 455 or
When second sheath clutch cone pulley 459 intervenes transmission group, corresponding first sheath stream driving cone pulley 456 or the second sheath stream drive cone pulley 4510
It starts to work, to drive corresponding rack-and-pinion to work.
It should be noted that clutch cone pulley is made of elastic material, surface can be set texture etc. and increase frictional force, this
Nylon or rubber material can be used in embodiment, on the top of the first sheath stream drive conical wheel 454 and the first sheath stream driving cone pulley 456
The bottom of portion and the second sheath stream drive conical wheel 458 and the second sheath stream driving cone pulley 4510 offers connection dimpled grain 4512 respectively,
The bottom of first sheath clutch cone pulley 455 and the top of the second sheath clutch cone pulley 459 offer corresponding connection burr respectively
4513, this design scheme, in the first sheath stream drive conical wheel 454 and the second sheath stream 458 fast idle of drive conical wheel, clutch cone
Wheel can be gradually in contact with it, and gradually be synchronized by frictional force, when connection dimpled grain 4512 and connection burr 4513 mesh together
When, it realizes and rotates synchronously, avoid skidding.
It should be further noted that the first sheath stream drive conical wheel 454, the first sheath stream drive cone pulley 456, the first sheath clutch
Cone pulley 455, the second sheath stream drive conical wheel 458, the second sheath stream driving cone pulley 4510 and the second sheath clutch cone pulley 459 can also use
Step cone pulley scheme, step cone pulley include the inner cone 4515 and rear cone 4516 for connecting table top 4514 and both ends at middle part,
Anti-skid chequer is provided on the connection table top 4514, anti-skid chequer can be straight-tooth, ripple glaze etc..
In the present embodiment, the specifically used method of above-mentioned micro-fluidic chip includes the following steps:
The installation of step 1 chip: the input terminal 10311 of main channel 1031 is connected to sample by sample plunger pump 42 in micro-fluidic chip
Pond, the first sorting channel 1036, second of micro-fluidic chip sort channel 1037 and third sorting channel 1038 and are respectively communicated with the
One sorting pool, the second sorting pool and third sorting pool, the first sheath circulation road 1032 and the second sheath circulation road of micro-fluidic chip
1033 connect the first sheath stream plunger pump 43, third sheath circulation road 1034 and the 4th sheath circulation road 1035 by hose is connected by hose
Lead to the second sheath stream plunger pump 44, the heating sheet 5 inside the first micro-valve door 1011, the second micro-valve door 1012 and third micro-valve door 1013
Pass through connection power-supply controller of electric；
Step 2 detectable substance focuses: focusing sheath stream group and is started to work with the flow rate of liquid of 8-12m/s, focuses sheath stream group work 5-10s
Afterwards, sample plunger pump starts to work and the detectable substance in sample cell is driven to enter main channel 1031, and detectable substance is focusing sheath stream group
Under the action of be gradually focused into single-row layout, until detectable substance enter observation section 10312 in, focus sheath stream group stop working；
It is controlled specifically, focusing sheath stream group by the first sheath stream plunger pump 43.
The analysis of step 3 detectable substance: after focusing sheath stream group stops working, detectable substance is stopped in observation section 10312, detection point
Analysis apparatus 201 obtains the physical aspect or spectral information of detectable substance, and point of detectable substance is determined based on physical aspect or spectral information
Class；
Specifically, testing and analyzing device 201 is high definition CCD, high-definition camera, Raman spectrum detecting device or fluorescence detection device
One of, the collection result for testing and analyzing device 201 is uploaded to host computer, host computer be based on physical aspect or spectral information into
The further analysis of row and comparison, to determine that detectable substance characteristic, physical aspect include size, profile and the transparency letter of detectable substance
Breath, spectral information includes Raman spectrum or fluorescence information, and detectable substance can be the chemical substance of cell, protein, fluorescent marker
Etc..
Step 4 sorting prepares: after testing and analyzing the classification information that device 201 determines detectable substance, focusing sheath stream group with 8-
The flow rate of liquid of 12m/s is started to work, and sheath stream group is driven to start to work with the flow rate of liquid of 8-12m/s, meanwhile, the first micro-valve door
1011, to be in non-heated condition, valve chamber unexpanded for corresponding micro-valve door in the second micro-valve door 1012 or third micro-valve door 1013, right
The sorting channel answered is opened, and the heating of heating sheet 5 of other micro-valve doors closes its corresponding channel, and sorting observation device 202 acquires
It drives sheath stream group and micro-valve door and sorts the working condition in channel, determine that correctly sorting channel is opened；
Specifically, sorting observation device 202 is high definition CCD or high-definition camera, specific determining work can be according to micro-valve door
Form determine, can also manually pass through naked-eye observation amplify acquisition image or video information determine.
The sorting of step 5 detectable substance: detectable substance enters determining sorting channel, sorting observation under the promotion of driving sheath stream group
The determining detectable substance of device 202 behind the door by corresponding micro-valve, start to work, and valve chamber starts to expand and close by the heating sheet 5 of micro-valve door
Corresponding valve is closed, determining sorting pool is entered under the promotion of gap miniflow by the detectable substance of micro-valve behind the door；
Specifically, detectable substance can be cell, protein, chemical industry microscopic material etc..
Step 6 executes step 2.
Specifically, gap miniflow is generated by the gap between micro-valve door and sorting channel, in design, micro-valve door is
Make entirely pneumatic, also can stay a part of gap between micro-valve door and corresponding sorting channel to generate gap miniflow and to push detection
Object falls into sorting pool.
It should be noted that entire control process needs that computer programming is combined to realize, it can also be real based on manual operation
It is existing, for example, the control of micro-valve door, miniflow pump and plunger pump tests and analyzes device 201 and sorting observation device by manual operation
202 acquisition amplification result is by artificial judgment, with the expansion of database and perfect, then be gradually transitioned into complete computer from
Dynamicization control.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. a kind of micro-fluidic chip, which is characterized in that micro-fluidic chip (1) include control layer (101), PDMS film layer (102),
Fluid channel layer (103) and substrate of glass (104), in which: be provided in the control layer (101) multiple for open and close fluid
The micro-valve door in channel, the micro-valve door are thermal expansion valve；1 main channel is provided in the fluid channel layer (103)
(1031), the input terminal (10311) of main channel (1031) is connected to sample cell, and main channel is provided with observation section in the middle part of (1031)
(10312), the output end (10313) of main channel (1031) is separately connected three sorting channels, sets above the sorting channel
It is equipped with the micro-valve door；It is symmetrically arranged between the input terminal (10311) and observation section (10312) and focuses sheath stream group；?
Driving sheath stream group is symmetrically arranged between observation section (10312) and sorting channel；
The focusing sheath stream group is for controlling detectable substance single-row layout and movement and including the first sheath circulation road (1032) and second
Sheath circulation road (1033), under the promotion of the first sheath circulation road (1032) and the second sheath circulation road (1033), detectable substance can be according to
It is secondary to enter observation section (10312)；The driving sheath stream group is for driving detectable substance to advance and including third sheath circulation road (1034)
With the 4th sheath circulation road (1035), under the promotion of third sheath circulation road (1034) and the 4th sheath circulation road (1035), detectable substance
According to being set into different sorting channels；
The sorting channel includes that the first sorting channel (1036), the second sorting channel (1037) and third sort channel
(1038), first sorting channel (1036), the second sorting channel (1037) and third sorting channel (1038) are respectively communicated with
Different sorting pools；
The micro-valve door includes the first micro-valve door (1011), the second micro-valve door (1012) and third micro-valve door (1013), in which: institute
The setting of the first micro-valve door (1011) is stated in the top of the first sorting channel (1036), the diminution or expansion of the first micro-valve door (1011)
(102) deformation of PDMS film layer can be driven to realize the on and off in the first sorting channel (1036), the second micro-valve door
(1012) diminution or expansion can drive (102) deformation of PDMS film layer with realize the second sorting channel (1037) conducting or
It closes, the diminution or expansion of third micro-valve door (1013) can drive (102) deformation of PDMS film layer to realize that third sorting is logical
The on and off in road (1038)；
The sheath circulation road and the angle of main channel (1031) are 72 °, and the two sides of the sheath circulation road import bevel edge and main channel
Angle be 16 °.
2. micro-fluidic chip according to claim 1, which is characterized in that the micro-valve door is internally provided with heating sheet
(3), the heating sheet (3) is made of alloy platinum material, and heating sheet (3) is using the mutually double helix shaped form knot of coiling and noninterlace
Structure, the outer profile width of the double helix shaped form structure are 700 μm.
3. micro-fluidic chip according to claim 2, which is characterized in that the valve chamber of the thermal expansion valve is using round knot
Structure, the radius of round valve chamber are 850 μm, close to the round valve chamber PDMS film layer (102) with a thickness of 120 μm.
4. micro-fluidic chip according to claim 3, which is characterized in that the control layer (101) is glass material, described
Valve chamber is provided on the control layer (101) by photoetching process, and the heating sheet (3) is arranged by sputtering technology at valve chamber bottom
Portion.
5. a kind of miniflow driving control system for micro-fluidic chip described in Claims 1-4 any one, feature exist
In, including observation device (2) and fluid control apparatus (4), in which:
The observation device (2), which is divided into, tests and analyzes device (201) and sorting observation device (202), the detection and analysis device
(201) it is arranged in the side of observation section (10312), tests and analyzes physical aspect or light that device (201) are used to obtain detectable substance
Spectrum information, sorting observation device (202) setting sort observation device (202) for anti-in the side of output end (10313)
The screening motion profile of detectable substance is presented, the physical aspect includes size, profile and the transparence information of detectable substance, the spectrum
Information includes Raman spectrum or fluorescence information；
The fluid control apparatus (4) includes cabinet (41), sample plunger pump (42), the first sheath stream plunger pump (43), the second sheath
Flow plunger pump (44) and transmission group (45), in which:
The cabinet (41) is internally provided with power supply and control panel (411), motor (412), the fixing seat for installing motor (412)
(413), multiple fixed bearing blocks (414), two groups of plain bearing housings (415), the sliding slot for installing plain bearing housing (415)
(416) and driving plain bearing housing (415) movement electromagnet (417) and reset spring (418)；
The sample plunger pump (42) includes sample cell (421), sample push rod (422), sample chock plug (423), sample rack gear
(424), sample inlet tube (425) and sample outlet tube (426), for receiving sample, the sample pushes away the sample cell (421)
One end of bar (422) is moveably mounted on cabinet (41), and the other end of sample push rod (422) is mounted on sample cell (421)
Interior, the sample chock plug (423) is used for sealed sample pipe (421) and is mounted on the other end of sample push rod (422), the sample
Chock plug (423) and sample cell (421) inner wall form sample cavity, and the sample inlet tube (425) and sample outlet tube (426) are respectively
It is connected to sample cavity, sample inlet tube (425) is connected to sample cell by hose, and sample outlet tube (426) is main logical by hose connection
Road (1031), the sample rack gear (424) are arranged on sample push rod (422), and sample rack gear (424) is driven by transmission group (45)
It is dynamic；
The first sheath stream plunger pump (43) includes the first sheath flow tube (431), the first sheath stream push rod (432), the first sheath stream chock plug
(433), the first sheath stream rack gear (434), the first sheath stream inlet tube (435) and the first sheath stream outlet tube (436), first sheath stream
For receiving sheath flow liquid, one end of the first sheath stream push rod (432) is moveably mounted on cabinet (41) pipe (431), the
The other end of one sheath stream push rod (432) is mounted in the first sheath flow tube (431), and the first sheath stream chock plug (433) is for sealing
First sheath flow tube (431) and the other end for being mounted on the first sheath stream push rod (432), the first sheath stream chock plug (433) and first
Sheath flow tube (431) inner wall forms the first sheath stream chamber, the first sheath stream inlet tube (435) and the first sheath stream outlet tube (436) point
It is not connected to the first sheath stream chamber, the first sheath stream inlet tube (435) is connected to sheath liquid pool, and the first sheath stream outlet tube (436) passes through hose one
It is divided into two connection the first sheath circulation roads (1032) and the second sheath circulation road (1033), the first sheath stream rack gear (434) setting exists
On first sheath stream push rod (432), the first sheath stream rack gear (434) is driven by transmission group (45)；
The second sheath stream plunger pump (44) includes the second sheath flow tube (441), the second sheath stream push rod (442), the second sheath stream chock plug
(443), the second sheath stream rack gear (444), the second sheath stream inlet tube (445) and the second sheath stream outlet tube (446), second sheath stream
For receiving sheath flow liquid, one end of the second sheath stream push rod (442) is moveably mounted on cabinet (41) pipe (441), the
The other end of two sheath stream push rods (442) is mounted in the second sheath flow tube (441), and the second sheath stream chock plug (443) is for sealing
Second sheath flow tube (441) and the other end for being mounted on the second sheath stream push rod (442), the second sheath stream chock plug (443) and second
Sheath flow tube (441) inner wall forms the second sheath stream chamber, the second sheath stream inlet tube (445) and the second sheath stream outlet tube (446) point
It is not connected to the second sheath stream chamber, the second sheath stream inlet tube (445) is connected to sheath liquid pool, and the second sheath stream outlet tube (446) passes through hose one
It is divided into two connection third sheath circulation roads (1034) and the 4th sheath circulation road (1035)；
The sample inlet tube (425), sample outlet tube (426), the first sheath stream inlet tube (435), the first sheath stream outlet tube
(436), manually-operated gate or solenoid valve are respectively arranged on the second sheath stream inlet tube (445) and the second sheath stream outlet tube (446)；
The transmission group (45) is for driving the first sheath stream plunger pump (43), the second sheath stream plunger pump (44) and sample plunger pump
(42) it works.
6. miniflow driving control system according to claim 5, which is characterized in that the transmission group (45) includes motor tooth
Take turns (451), sample reduction gearing (452), sheath stream reduction gearing (453), the first sheath stream drive conical wheel (454), the first sheath clutch
Cone pulley (455), the first sheath stream driving cone pulley (456), the first sheath stream driving gear (457), the second sheath stream drive conical wheel (458),
Second sheath clutch cone pulley (459), the second sheath stream driving cone pulley (4510) and the second sheath stream driving gear (4511), in which:
The motor gear (451) is set in motor (412) shaft, and electricity is engaged in the side of the sample reduction gearing (452)
Machine gear (451)；
Sample rack gear (424) are engaged in the side of sample reduction gearing (452), and the sheath stream reduction gearing (453), the first sheath are spread
Mantle wheel (454) and the second sheath stream drive conical wheel (458) are coaxially arranged, sheath stream reduction gearing (453) gearing motor gear
(451), the first sheath stream drive conical wheel (454) and the second sheath stream drive conical wheel (458) are arranged in the two of sheath stream reduction gearing (453)
Side；
The first sheath clutch cone pulley (455) is mounted on plain bearing housing (415), and first sheath stream drives cone pulley (456)
It is mounted on first sheath clutch cone pulley (455) side, can be realized the first sheath stream drive conical wheel by mobile plain bearing housing (415)
(454), the engagement or separation between the first sheath clutch cone pulley (455) and the first sheath stream driving cone pulley (456), first sheath stream
Drive gear (457) and the first sheath stream driving cone pulley (456) coaxial arrangement, first sheath stream driving gear (457) engagement the
One sheath stream rack gear (434)；
The second sheath clutch cone pulley (459) is mounted on plain bearing housing (415), and second sheath stream drives cone pulley (4510)
It is mounted on second sheath clutch cone pulley (459) side, can be realized the second sheath stream drive conical wheel by mobile plain bearing housing (415)
(458), the engagement or separation between the second sheath clutch cone pulley (459) and the second sheath stream driving cone pulley (4510), second sheath
Stream driving gear (4511) and the second sheath stream driving cone pulley (4510) coaxial arrangement, the second sheath stream driving gear (4511) are nibbled
Close the second sheath stream rack gear (434).
7. miniflow driving control system according to claim 6, which is characterized in that the first sheath stream drive conical wheel
(454) and the first sheath stream driving cone pulley (456) top and the second sheath stream drive conical wheel (458) and the second sheath stream driving bore
The bottom of wheel (4510) offers connection dimpled grain (4512), the bottom and second of the first sheath clutch cone pulley (455) respectively
The top of sheath clutch cone pulley (459) offers corresponding connection burr (4513) respectively.
8. miniflow driving control system according to claim 6, which is characterized in that the first sheath stream drive conical wheel
(454), the first sheath stream drives cone pulley (456), the first sheath clutch cone pulley (455), the second sheath stream drive conical wheel (458), the second sheath
Stream driving cone pulley (4510) and the second sheath clutch cone pulley (459) are step cone pulley, and the step cone pulley includes the connection at middle part
Table top (4514) and the inner cone (4515) and rear cone (4516) at both ends are provided on the connection table top (4514) anti-skidding
Line.
9. miniflow driving control system according to claim 5, which is characterized in that the reset spring (418) is pressure spring,
When electromagnet (417) is not powered on, reset spring (418) drives the first sheath clutch cone pulley (455) or the second sheath clutch cone pulley
(459) far from drive conical wheel group, when electromagnet (417) are powered, magnetic field and be arranged in sliding bearing that electromagnet (417) generates
The identical generation repulsion of the permanent magnet field of seat (415) pushes the first sheath clutch cone pulley (455) or the second sheath clutch cone pulley (459) to nibble
Close drive conical wheel group.
10. miniflow driving control system according to claim 5, which is characterized in that the reset spring (418) is to draw
Spring, when electromagnet (417) is not powered on, reset spring (418) drives the first sheath clutch cone pulley (455) or the second sheath clutch cone pulley
(459) engaged transmission cone pulley group, when electromagnet (417) are powered, magnetic field and be arranged in sliding bearing that electromagnet (417) generates
The permanent magnet field of seat (415) generates suction on the contrary and pushes the first sheath clutch cone pulley (455) or the second sheath clutch cone pulley (459) remote
From drive conical wheel group.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110639630A (en) * | 2019-09-23 | 2020-01-03 | 四川长虹电器股份有限公司 | Passive microfluidic chip structure for separating particles with different particle sizes |
| CN111208282A (en) * | 2020-01-14 | 2020-05-29 | 安徽奇书生物科技有限公司 | Multi-channel mixed flow injection type perfusion microscope |
| CN114728281A (en) * | 2019-10-18 | 2022-07-08 | 瞬知（广州）健康科技有限公司 | System and method for infusing fluid |
| CN115041243A (en) * | 2022-05-19 | 2022-09-13 | 珠海大略科技有限公司 | Micro-fluidic device for particle sorting and high concentration based on micropores |
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| CN111208282A (en) * | 2020-01-14 | 2020-05-29 | 安徽奇书生物科技有限公司 | Multi-channel mixed flow injection type perfusion microscope |
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| CN115041243B (en) * | 2022-05-19 | 2023-11-10 | 珠海大略科技有限公司 | Micro-fluidic device for particle sorting and high concentration based on micropores |
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