CN110180611B - Microfluidic chip assembly - Google Patents
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- CN110180611B CN110180611B CN201910605288.8A CN201910605288A CN110180611B CN 110180611 B CN110180611 B CN 110180611B CN 201910605288 A CN201910605288 A CN 201910605288A CN 110180611 B CN110180611 B CN 110180611B
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
Abstract
The application discloses a microfluidic chip assembly. The microfluidic chip assembly includes: comprises a fixed component and a plurality of chips; the fixing component is used for fixing the chips; the chip comprises a sample processing liquid adding port, a flow channel, a first collecting tank, a sample adding tank, a first micro-flow channel, a second micro-flow channel, a third micro-flow channel, a reaction bin and a sample processing bin; the sample processing liquid adding port is used for adding sample processing liquid; the sample adding groove is used for adding a sample; the sample processing liquid adding port is communicated with the sample processing bin through the flow channel and the first micro-flow channel; the sample adding groove is communicated with the sample processing bin through the second micro-channel; the sample treatment fluid and the sample can respectively enter the sample treatment bin through the first micro flow channel and the second micro flow channel under the action of the first centrifugal force and are mixed, and the mixed fluid after mixing can enter the reaction bin through the third micro flow channel to react.
Description
Technical Field
The application relates to the technical field of sample detection, in particular to a microfluidic chip assembly.
Background
With the progress and development of science, means of inspection and analysis are gradually developed in the technical field of sample detection and various subdivision fields thereof, such as biomedical analysis, disease diagnosis, soil detection, water detection, and the like, and certain progress is made in the fields of integration, portability, miniaturization, and the like.
Microfluidic chip technology is a technology for precisely controlling a small amount of liquid in a micrometer-scale flow channel, and is an important processing platform nowadays. The method is applied to the fields such as biomedical analysis, disease diagnosis and the like, and simultaneously meets the requirements of integration, portability, microminiaturization and the like.
The micro-fluidic chip technology is based on the chip as an operation platform and the analytical chemistry as a basis, the micro-electromechanical processing technology as a support, the micro-pipeline network as a structural feature and the life science as a main application object at present, and is an important point of development in the field of the current micro-total analysis system. Its goal is to integrate the functions of whole laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc. on microchip and to use it several times.
The traditional microfluidic chip consists of a substrate and a cover plate, wherein the substrate is provided with a micro-channel, and the cover plate encapsulates the substrate provided with the micro-channel, so that the micro-channel is in a relatively sealed state. When the micro-channel chip is applied to optical detection, a sample to be detected and a reagent are reflected in the lengthened inner area, and substances generated after detection reaction are detected by an optical method.
The centrifugal force driving is to utilize centrifugal force generated when the chip is driven by the micro motor to do circular motion as driving force of liquid flow, and to adjust and control dynamic characteristics of fluid by changing rotation speed of the chip and setting different channel configurations. The drive may be performed using an existing rotating platform, such as an optical disk drive. Fig. 12 is a schematic diagram of a conventional rotating platform, as shown in fig. 12, the rotating platform includes a disc body 100 and a rotating shaft 200, the rotating shaft 200 can rotate under the driving of a motor, the disc body 100 can rotate under the driving of the rotating shaft 200, so that a microfluidic chip assembly disposed on the disc body 100 rotates, and liquid driving and detection are realized by using centrifugal force.
The centrifugal force driven micro-fluidic chip detection method has the characteristics of controllable liquid flow, extremely small consumption of samples and reagents, ten times or hundreds of times higher analysis speed and the like, can be used for simultaneously analyzing hundreds of samples in a period of minutes or even shorter, and can realize the pretreatment and the whole analysis process of the samples on line. However, the design of the current microfluidic chip still needs to be advanced, for example, problems of unreasonable structural arrangement, imperfect functions and the like are all problems which need to be solved by the industry.
Disclosure of Invention
In view of the above, an embodiment of the present invention provides a microfluidic chip assembly to solve the problems of the prior art.
In order to solve the above problems, an embodiment of the present application discloses a microfluidic chip assembly including a fixing assembly and a plurality of chips (30); the fixing assembly is used for fixing the chips (30); the chip comprises a sample processing liquid adding port (31), a flow channel (32), a first collecting groove (33), a sample adding groove (34), a first micro-flow channel (35), a second micro-flow channel (35 a), a third micro-flow channel (36), a reaction bin (38) and a sample processing bin (39);
the sample processing liquid adding port (31) is used for adding a sample processing liquid; the sample adding groove (34) is used for adding a sample; the sample processing liquid adding port (31) is communicated with the sample processing bin (39) through the flow channel (32) and the first micro flow channel (35);
the sample adding groove (34) is communicated with the sample processing bin (39) through the second micro-flow channel (35 a); the sample treatment fluid and the sample can enter the sample treatment bin (39) respectively through the first micro flow channel (35) and the second micro flow channel (35 a) under the action of the first centrifugal force and are mixed, and the mixed fluid after mixing can enter the reaction bin (38) for reaction through the third micro flow channel (36).
In an embodiment, the chip further comprises a first collection tank (33), the first collection tank (33) being in communication with the flow channel (32) for collecting excess sample processing fluid.
In an embodiment, the chip further comprises a second collection tank (37) for collecting the liquid, the second collection tank (37) comprising a deep tank (37 a) and a shallow tank (37 b).
In an embodiment, the chip further comprises a fourth micro flow channel (38 a), the fourth micro flow channel (38 a) being configured to communicate the third micro flow channel (36) with the reaction chamber (38).
In one embodiment, the chip further comprises a third collection tank (39 a) connected to the third microchannel (36) for collecting waste liquid.
In one embodiment, the third fluidic channel (36) is a U-shaped channel, the bottom end of the U being closer to the center of rotation of the chip than the sample processing cartridge (39).
In one embodiment, the securing assembly includes: a fixing bolt (10), an upper cover (20) and a base (40);
the upper cover (20) and the base (40) are used for fixing the chips (30) from two sides of the chips (30); the fixing bolt (10) is used for connecting the upper cover (20), the base (40) and the chip (30).
In one embodiment, the fixing assembly further comprises a base fixing member (50), and the base fixing member (50) is used for being connected and fixed with the fixing bolt (10).
In an embodiment, the chip (30) has a flange (30 d), the base (40) has a base body (41), and the flange (30 d) and the base body (41) are receivable in the upper cover (20).
In one embodiment, the upper cover has a first rib (22) and a first press block (23); the base (40) has a second rib (42) and a second press block (43); the chips (30) are combined to form a circle, a first space (30 a) and a second space (30 b) are formed at the adjacent positions of the chips, and the positions of the first spaces (30 a) correspond to the first ribs (22) and the second ribs (42); the second space (30 b) is located in correspondence with the first press block (23) and the second press block (43) such that the first rib (22) and the second rib (42) can be at least partially accommodated within the first space (30 a), and the first press block (23) and the second press block (43) can be at least partially accommodated within the second space (30 b).
From the above, the microfluidic chip assembly provided in the embodiments of the present application enables the sample in the microfluidic chip to react under the drive of centrifugal force through the improved structural design on the microfluidic chip, thereby realizing detection. The microfluidic chip assembly is reasonable in design, simple to install, capable of achieving quick assembly disassembly, capable of achieving quick and accurate detection effect in cooperation with centrifugal force driven modes such as a rotating platform.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a diagram showing the overall assembly and disassembly of a microfluidic chip assembly according to one embodiment of the present application;
FIG. 2 is a diagram showing an overall assembly and combination of microfluidic chip assemblies according to one embodiment of the present application;
FIG. 3 is an assembled perspective view of a bolt and base mount of a microfluidic chip assembly according to one embodiment of the present application;
fig. 4 is an assembled perspective view of a bolt and base fixture of a microfluidic chip assembly according to one embodiment of the present application;
fig. 5 is a perspective view of an upper cover of a microfluidic chip assembly according to an embodiment of the present application;
fig. 6 is a perspective view of a base of a microfluidic chip assembly according to an embodiment of the present application;
fig. 7 is a schematic perspective view of a microfluidic chip assembly according to an embodiment of the present disclosure;
FIG. 8 is a perspective view of a microfluidic chip assembly according to one embodiment of the present disclosure;
fig. 9 is a plan view of a mixing tank 1 of a microfluidic chip assembly according to an embodiment of the present application;
FIG. 10 is a cross-sectional view of a mixing tank of a microfluidic chip assembly according to one embodiment of the present application;
fig. 11 is a cross-sectional view of a mixing tank of a microfluidic chip assembly according to an embodiment of the present application.
Fig. 12 is a schematic diagram of a prior art rotating platform that mates with the rotation of a microfluidic chip assembly.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application are within the scope of the protection of the present application.
The embodiment of the invention provides a microfluidic chip assembly driven by centrifugal force. In one embodiment, the microfluidic chip assembly cooperates with a rotating platform to detect samples. Referring to fig. 12, the microfluidic chip assembly may be disposed on a rotating platform shown in fig. 12, and the rotating platform is driven by a motor to drive the microfluidic chip assembly to rotate, so as to realize liquid flow and mixing detection in the microfluidic chip.
Fig. 1 is a schematic diagram illustrating an assembly structure of a microfluidic chip assembly according to an embodiment of the present invention, and fig. 2 is a schematic diagram illustrating an assembly corresponding to fig. 1. As shown in fig. 1 and 2, a microfluidic chip assembly according to an embodiment of the present application may include a fixing bolt 10, an upper cover 20, a chip 30, and a base 40. In the above structure, the upper cover 20 and the base 40 clamp the chip 30 from both sides of the chip 30, respectively, and the fixing bolt 10 is used for fixing the upper cover 20 and the base 40, and in addition, the fixing bolt 10 can fix the chip 30 on the aforementioned rotating platform, so that the chip 30 is fixed with the rotating platform, and the chip 30 is prevented from being thrown out in the radial direction under the action of centrifugal force.
The number of chips 30 may be plural, and in this embodiment, 8 sectors are shown, and the 8 chips are separated from each other, and combined together, may form a disk shape. The fixing pins 10, the upper cover 20, and the base 40 may be used as fixing components to fix the plurality of chips 30 to the rotating platform.
The specific structure of the microfluidic chip assembly is described below. It is noted that the drawings of the present invention are only for illustrating the connection manner of the parts in the assembly, and are not limited to the specific shapes and proportions among the parts, etc.
As shown in fig. 1, the fixing bolt 10 may pass through the opening of the upper cover 20, the screw of the fixing bolt 10 may extend downward all the way through the opening formed by the plurality of chips 30 and the opening of the base 40 until being matched with the base fixing member 50 (see fig. 3 and 4) below the base 40, relatively fixing the upper cover 10, the chips 30 and the base 40 of the microfluidic chip assembly, and fixing the fixed microfluidic chip assembly on the rotating table, so that the structures can rotate synchronously with the rotating table. The fixing mode can be bolt fixing, clamping fixing, embedding fixing, interference fit fixing and the like, and is not described herein.
Fig. 3 and 4 are perspective and perspective views showing the mating structure of the fixing bolt 10 and the base fixing member 50, respectively, according to an embodiment of the present application, and the upper cover 10, the chip 30 and the base 40 are omitted from fig. 3 and 4 so as to clearly show the mating structure of the fixing bolt 10 and the base fixing member 50.
As shown in fig. 3 and 4, the fixing bolt 10 includes a bolt head 11 and a rod body 12, and the rod body 12 may have a convex portion 12a thereon; the base fixture 50 includes a column and a flange 52 in the middle of the column, wherein the column may be divided into an upper column 51 and a lower column 53 by the flange 52. The post may have a through hole 51a, with a receiving groove 51b in the through hole 51a that mates with the raised portion 12a of the rod body 12. When assembling, by applying force to the fixing bolt 10 or the base fixing member 50, the convex portion 12a and the receiving groove 51b are deformed by the force, and are engaged with each other, so that the rod body 12 of the fixing bolt 10 is tightly fitted.
In fig. 3 and 4, the fixing bolt 10 and the base fixing member 50 are engaged by means of a snap-fit, and in other embodiments, the fixing bolt 10 may be threaded, and the base fixing member 50 may be correspondingly threaded, so that the two are engaged by means of a screw. There are also various ways in the art that the microfluidic chip assembly may be fixed, and these ways are not described in detail herein.
Fig. 5 is a perspective view of the upper cover 20, and as shown in fig. 5, the upper cover 20 has a body 21 and radial first ribs 22. As shown in fig. 2 and 5, the body 21 is similar to a bottle cap and includes an opening 20a, and is partially hollow, wherein the hollow portion is used for accommodating a flange of the chip 30 and a flange of the base 40.
The first rib 22 is configured to radially extend from the body 21 of the upper cover 20, and the end of the first rib 22 has a first pressing block 23, and the edge of the upper cover 20 further has an annular structure 24 concentrically disposed with the body 21.
Fig. 6 is a schematic view of the base 40, and as shown in fig. 6, the base 40 has a base body 41, a second rib 42 and a second pressing block 43 corresponding to the upper cover 20, and a base edge 44. The base body 41 has an opening 40a at the center thereof, the opening 40a is capable of allowing the upper column 51 of the base fixing member 50 to pass therethrough, and the opening 40a is smaller than the flange 52, so that the flange 52 is not allowed to pass therethrough.
Fig. 7 is a schematic diagram of chips 30 according to an embodiment of the present application, and as shown in fig. 7, a group of 8 chips 30 in this embodiment are formed by splicing 8 fan-shaped chips together to form a circle, and after the splicing, a first space 30a and a second space 30b are provided between the two chips 30. As shown in connection with fig. 2, the first space 30a is positioned to correspond to the first rib 22 and the second rib 42; the second space 30b is positioned to correspond to the first and second press blocks 23, 43 such that the first and second ribs 22, 42 are at least partially receivable within the first space 30a and the first and second press blocks 23, 43 are at least partially receivable within the second space 30b.
As shown in fig. 7, each of the chips 30 includes a body 30c, a flange 30d, and a micro flow path structure 30e, the micro flow path structure 30e being provided on the body 30c, the flange 30d being provided at one end of the body 30c for being fixed by the upper cover 20 and the base 40.
Fig. 8 is a schematic view showing the structure of each part of the micro flow channel structure 30e of the chip 30, and fig. 9 is a front view of the fan-shaped chip 30. Fig. 10 is an enlarged schematic view of the portion H in fig. 9. Fig. 11 is a sectional view A-A in fig. 10.
As shown in fig. 8, the micro flow channel structure 30e includes: the sample processing liquid adding port 31, the flow channel 32, the first collecting tank 33, the sample adding tank 34, the first micro flow channel 35, the second micro flow channel 35a, the third micro flow channel 36, the second collecting tank 37, the reaction chamber 38, the fourth micro flow channel 38a, the sample processing chamber 39, and the third collecting tank 39a.
The sample processing liquid addition port 31 is provided at one end of the chip 30 near the center of the circle, i.e., at one end near the flange 30 d. The sample processing liquid addition port 31 is used for adding a sample processing liquid. The sample treatment liquid is used for treating the sample. After addition, when the rotary stage is rotated, the chip 30 causes the sample processing liquid to flow along the flow path 32 by centrifugal force, and the surplus sample processing liquid is collected in the first collection tank 33. It is noted that in some embodiments, when the width of the flow channel 32 is sufficient, the sample processing liquid may naturally flow through the flow channel 32 without applying centrifugal force. In other cases, the flow channel 32 may be a micro-channel, which requires liquid to flow internally by centrifugal force actuation.
The sample addition slot 34 is used to add a test sample. Under the action of the first centrifugal force, the sample processing liquid and the test sample flow along the corresponding first micro flow channel 35 and the second micro flow channel 35a, respectively, and are mixed and reacted in the sample processing chamber 39. In some embodiments, the first micro flow channel 35 and the second micro flow channel 35a limit the mixing speed, and the mixing speed can be limited by the design of the size; in other embodiments, the first micro flow channel 35 and the second micro flow channel 35a may be flow-restricting micro flow channels, and the mixing speed may be restricted by the design of the size.
When the mixed liquid in the sample processing cartridge 39 overflows, it flows into the second collection tank 37 through the third micro flow channel 36. The second collection tank 37 may be a waste collection tank for collecting waste. As shown in fig. 8, the second collecting tank 37 may include two parts, one part being a deep tank 37a and one part being a shallow tank 37b. The deep groove 37a is used for collecting waste liquid, and the shallow groove 37b is used for realizing buffering. The deep groove is closer to the third fluidic channel 36 than the shallow groove, i.e. liquid will first enter the deep groove.
The third micro flow channel 36 is used for intercepting liquid under the condition of the first centrifugal force and preventing overflow; the third fluidic channel 36 may be configured as a U-shaped channel with the bottom end of the U-shape being radially closer to the flange 30d than the sample processing cartridge 39, i.e. the center of rotation of the chip 30 after assembly, e.g. the center of the rotation platform. Through the structure setting of U-shaped, play the effect that prevents gaseous convection, prevent that liquid from spilling over by the reaction tank.
The structure of the sample processing cartridge 39 can be seen in fig. 9 to 11. The sample processing cartridge 39 has a stepped shape structure for achieving buffering of the liquid.
The reaction chamber 38 is used for reacting the mixed liquid entering the reaction chamber 38 through the third micro flow channel 36 and the fourth micro flow channel 38a in the sample processing chamber 39 by photochemical reaction and the like, and displaying the reaction result. The mixed liquid in the sample processing chamber 39 can enter the reaction chamber 38 through the third and fourth microchannels 36 and 38a by the action of the second centrifugal force. The first centrifugal force and the second centrifugal force may be set to be different in order to control the flow of the liquid. The first centrifugal force may be less than the second centrifugal force. In chip processing, the resistance of liquid flow can be increased or reduced by setting the width of the micro flow channel, and when the centrifugal force does not exceed a certain threshold value, the liquid cannot pass through the micro flow channel; only when the centrifugal force is greater than a certain threshold value, the centrifugal force can enable the liquid to pass through the corresponding micro-flow channel. In chip processing, a thin film capable of providing corresponding resistance can be arranged in the micro-channel, and the corresponding thin film can be broken only when the centrifugal force exceeds a certain threshold value, so that liquid can flow through the corresponding micro-channel. In practice, those skilled in the art will have other arrangements, which will not be described in detail herein.
The reaction cartridge 38 may store lyophilized reagents such as enzyme preparations, antigen antibodies, microspheres, magnetic particles, and may entrap conjugated carriers such as antigens, antibodies, proteins, lipids, nucleic acids, and the like that have binding detection effects. The reaction chambers 38 may be single or multiple, and when there are multiple reaction chambers 38, different reagents may be stored in each reaction chamber 38 for different reactions with respect to the same mixed liquid.
The third microchannel 36 is further connected to a third collecting tank 39a. The third collection tank 39a may be a waste collection tank, which functions at least in: 1, collecting a small amount of liquid overflowed from a micro-channel, 2, collecting liquid which remains in a pipeline at the front end and is not fully treated, and 3, collecting liquid of which the front end is used for pipeline washing.
Through the structure, the microfluidic chip assembly provided by the embodiment of the application enables the sample in the microfluidic chip to react under the drive of centrifugal force through the improved structural design on the microfluidic chip, so that detection is realized. The microfluidic chip assembly is reasonable in design, simple to install, capable of achieving quick assembly disassembly, capable of achieving quick and accurate detection effect in cooperation with centrifugal force driven modes such as a rotating platform.
While preferred embodiments of the present embodiments have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the embodiments of the present application.
Finally, it is further noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article or terminal device comprising the element.
Claims (9)
1. A microfluidic chip assembly comprising a fixation assembly and a plurality of chips (30); the fixing assembly is used for fixing the chips (30); the chip comprises a sample processing liquid adding port (31), a flow channel (32), a first collecting groove (33), a sample adding groove (34), a first micro-flow channel (35), a second micro-flow channel (35 a), a third micro-flow channel (36), a reaction bin (38) and a sample processing bin (39);
the sample processing liquid adding port (31) is used for adding a sample processing liquid; the sample adding groove (34) is used for adding a sample; the sample processing liquid adding port (31) is communicated with the sample processing bin (39) through the flow channel (32) and the first micro flow channel (35);
the sample adding groove (34) is communicated with the sample processing bin (39) through the second micro-flow channel (35 a); the sample treatment fluid and the sample can enter the sample treatment bin (39) respectively through a first micro-channel (35) and a second micro-channel (35 a) under the action of a first centrifugal force and are mixed, the mixed fluid can enter the reaction bin (38) to react through the third micro-channel (36) under the action of a second centrifugal force, and the first centrifugal force is smaller than the second centrifugal force;
the fixing assembly includes: a fixing bolt (10), an upper cover (20) and a base (40);
the plurality of chips (30) are combined together, and the upper cover (20) and the base (40) clamp the chips (30) from two sides of the plurality of chips (30) respectively and are used for fixing the chips (30) from two sides of the plurality of chips (30); the upper cover (20) and the base (40) are respectively provided with an opening, and the fixing bolt (10) penetrates through the opening of the upper cover (20) and the opening of the base (40) and is used for connecting the upper cover (20), the base (40) and the chip (30).
2. The microfluidic chip assembly according to claim 1, wherein the chip further comprises a first collection tank (33), the first collection tank (33) being in communication with the flow channel (32) for collecting excess sample processing liquid.
3. The microfluidic chip assembly according to claim 2, wherein the chip further comprises a second collection tank (37) for collecting liquid, the second collection tank (37) comprising a deep tank (37 a) and a shallow tank (37 b).
4. A microfluidic chip assembly according to claim 3, wherein the chip further comprises a fourth microchannel (38 a), the fourth microchannel (38 a) being adapted to communicate the third microchannel (36) with the reaction cartridge (38).
5. The microfluidic chip assembly according to claim 4, wherein the chip further comprises a third collection tank (39 a) connected to the third micro flow channel (36) for collecting waste liquid.
6. The microfluidic chip assembly according to claim 1, wherein the third fluidic channel (36) is a U-shaped channel with the bottom end of the U-shape of the U-shaped channel being closer to the center of rotation of the chip than the sample processing cartridge (39).
7. The microfluidic chip assembly according to claim 1, wherein the fixation assembly further comprises a base fixation (50), the base fixation (50) being adapted to be connected and fixed with the fixation peg (10).
8. The microfluidic chip assembly according to claim 7, wherein the chip (30) has a flange (30 d), the base (40) has a base body (41), the flange (30 d) and the base body (41) being receivable in a hollow of the body (21) of the upper cover (20); the flange (30 d) of the chip (30) is arranged at one end of the chip (30), the position of the base body (41) on the base (40) corresponds to the flange (30 d) of the chip (30), and an opening (40 a) for the base fixing piece (50) to pass through is formed in the center of the base body (41).
9. The microfluidic chip assembly according to claim 8, wherein the upper cover has a first rib (22) and a first press block (23); the base (40) has a second rib (42) and a second press block (43); the chips (30) are combined to form a circle, a first space (30 a) and a second space (30 b) are formed at the adjacent positions of the chips, and the positions of the first spaces (30 a) correspond to the first ribs (22) and the second ribs (42); the second space (30 b) is located in correspondence with the first press block (23) and the second press block (43) such that the first rib (22) and the second rib (42) can be at least partially accommodated within the first space (30 a), and the first press block (23) and the second press block (43) can be at least partially accommodated within the second space (30 b).
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| CN112791645B (en) * | 2021-01-26 | 2022-05-13 | 驻马店职业技术学院 | Fluid-driven control valve |
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