CN110179903B - Application of aquilaria sinensis extract in reducing skin inflammation reaction caused by irradiation of ultraviolet light on keratinocytes and promoting skin cutin metabolism - Google Patents
Application of aquilaria sinensis extract in reducing skin inflammation reaction caused by irradiation of ultraviolet light on keratinocytes and promoting skin cutin metabolism Download PDFInfo
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Abstract
The invention relates to application of a water fragrant edge extract, in particular to application of the water fragrant edge extract in reducing skin inflammation reaction caused by ultraviolet light irradiation of keratinocytes and promoting skin cutin metabolism. The water fragrant arris extract is obtained by extracting water, alcohol or a solvent of a mixture of alcohol and water, and can reduce skin inflammation reaction of skin keratinocytes caused by ultraviolet irradiation, promote skin keratinocyte metabolism, improve skin texture and pore size and reduce melanin content.
Description
Technical Field
The invention relates to application of a water fragrant edge extract, in particular to application of the water fragrant edge extract in preparing a medicinal composition for reducing skin inflammation reaction caused by ultraviolet light irradiation of keratinocytes and promoting skin cutin metabolism.
Background
The stratum corneum (stratum corneum) is located in the outermost layer of the epidermis of the skin and has the primary function of retaining water and helping the skin to resist various environmental insults. The stratum corneum consists of 15 to 20 layers of keratinocytes, which are dead cells without nuclei and organelles and have a main component of keratin (keratin) that absorbs water to keep the skin moist. After a certain period of time, keratinocytes are shed by themselves, keratinized by cells below the stratum corneum, and advanced upward to form a new stratum corneum.
Old waste cells in the horny layer can fall off by themselves under normal conditions, but the old waste horny cells can be accumulated to lose the capability of natural metabolism due to environmental factors, and at the moment, the appearance of the skin can be dull, dull and rough and unsmooth. In order to restore the normal metabolism of keratin, there are many exfoliating compositions and methods on the market, however, after artificial exfoliation, although the skin becomes smooth and shiny, the skin becomes fragile, sensitive and the water retention capacity is reduced because the stratum corneum becomes thin.
In addition, UVB in uv light has high energy, penetrates into the skin epidermis to cause sunburn of the skin, and causes symptoms such as pachynsis, thickening of the horny layer, and inflammation, and once sunburn is severe, the skin in the area is damaged and becomes fragile and easily spotted.
In order to improve the current treatment mode of poor metabolism of old waste keratinocytes; the skin is red, swollen, inflamed and thickened after being irradiated by ultraviolet; and the stratum corneum becomes thin and damaged, so that the skin becomes fragile, sensitive and rough, and the development of a natural skin-refreshing medicinal composition capable of promoting the metabolism of keratinocytes, resisting skin inflammation and increasing the skin protection capability is essential.
Disclosure of Invention
Accordingly, an object of the present invention is to provide a use of a aquilaria extract for preparing a pharmaceutical composition for promoting skin cutin metabolism.
Still another object of the present invention is to provide a use of a aquilaria sinensis extract for preparing a pharmaceutical composition for reducing inflammatory reaction of keratinocytes caused by irradiation with ultraviolet light.
Still another object of the present invention is to provide a use of a aquilaria sinensis extract for preparing a pharmaceutical composition for reducing inflammatory reaction of keratinocytes caused by irradiation with ultraviolet light.
The water fragrant arris extract is obtained by extracting water fragrant arris with a solvent, wherein the solvent is water, alcohol or an alcohol-water mixture, and the liquid-solid ratio of the solvent to the water fragrant arris is 5-20: 1-5, and the extraction step is carried out at 50-100 ℃.
In one embodiment of the present invention, the concentration of the water incense stick extract is at least 1.25mg/mL, and the water incense stick extract promotes keratinocyte proliferation.
In one embodiment of the present invention, the ultraviolet light is a UVB, the water fragrant ridge extract inhibits secretion of interleukin 8 (IL-8) by the keratinocytes, and the concentration of the water fragrant ridge extract is at least 0.0625 mg/mL.
The following description of the present invention is provided in connection with the accompanying drawings, which are included to illustrate and not to limit the scope of the present invention, and it will be understood by those skilled in the art that various changes and modifications may be made therein without departing from the spirit and scope of the invention, and it is intended to cover all modifications and equivalents as may fall within the true spirit and scope of the invention.
Drawings
FIG. 1 is a histogram of the effect of the extract of the water incense stick of the present invention on promoting the proliferation of skin keratinocytes; p <0.05 compared to the blank control group;
FIG. 2 is a histogram of the reduction of interleukin 8 secretion, an inflammatory factor, of the extract of Satureja johnsonii according to an embodiment of the present invention; p <0.001 compared to the positive control group p < 0.01; compare to control group # p < 0.001;
FIG. 3 is a histogram of the effect of the water incense stick extract of the present invention on improving skin texture;
FIG. 4 is a histogram of the effect of the water incense stick extract of the present invention on reducing the size of skin pores;
FIG. 5 is a histogram of the effect of the extract of Satureja Sauropus on reducing the melanin content of the skin according to one embodiment of the present invention; p <0.05 compared to before use.
Detailed Description
The invention provides a water fragrant arris extract for reducing skin inflammation reaction caused by irradiation of ultraviolet light on keratinocytes, promoting skin cutin metabolism, improving skin texture and pore size and reducing melanin content. The following examples show that the extract of the present invention can promote keratinocyte proliferation, inhibit the secretion of UVB-induced inflammatory factor IL-8, and improve skin texture, pore size and reduce melanin content.
As used herein, the numerical values are approximations and all numerical data are reported to be within the 20 percent range, preferably within the 10 percent range, and most preferably within the 5 percent range.
Example 1 preparation of the extract of saussurea Clarke according to the present invention
Water fragrant arris (Cyperusrotundus) is a plant of Cyperaceae (Cyperaceae), also known as cyperus rotundus, native fragrance, smelly head fragrance and the like, and has the effects of relieving pain, regulating menstruation, dispelling cold hernia and relieving abdominal pain.
In one embodiment of the present invention, the whole plant of the water fragrant arris is cleaned, and the cleaned whole plant of the water fragrant arris and an extraction solvent of water, alcohol or a mixture of alcohol and water are mixed in a ratio of 5-20: 1-5, homogenizing, and extracting at 50-100 deg.C, preferably 75-95 deg.C for 0.5-3 hr to obtain crude extract. After extraction, the mixture was cooled to room temperature, and the crude extract was filtered through a 400-mesh sieve to obtain a filtrate. Finally, the filtrate is decompressed and concentrated at 45-70 ℃ to obtain the water fragrant arris extract liquid of the invention.
Example 2 Effect of the extract of Aquilaria sinensis of the present invention on promoting the proliferation of skin keratinocytes
The invention uses human primary epidermal keratinocyte (HPEKp) to perform the experiment of skin keratinocyte proliferation. The human primary skin keratinocytes were purchased from CELLnTEC, Inc. (Switzerland) under the number HPEK-50. The cells were cultured in serum-free keratinocyte medium (Keratinocyte-SFM) (Gibco, USA, No. # 10724-011).
The assay was performed using a cell proliferation reagent kit (purchased from Roche, switzerland, No. 11647229001). First, in a 96-well culture plate, 3X 10 cells were implanted per well 3 Human primary skin keratinocytes were cultured in the above culture medium at 37 ℃ for 2 hours, 100. mu.l of a blank control (mock) or 1.25mg/mL of the water toilet web extract of the present invention and 10. mu.l of 100. mu.M of Bromodeoxyuridine (BrdU) were added to each well, followed by culture at 37 ℃ for 24 hours, and the proliferation of the cells was detected by the amount of inserted DNA in BrdU. The culture medium was removed without disturbing the adherent cells, and 200. mu.l of a fixative (FixDenat, visual #2) was added to each well and allowed to act at room temperature for 30 minutes. After removal of the fixative, the gel was washed once with Phosphate Buffered Saline (PBS). BrdU was identified by adding 100. mu.l of anti-BrdU-POD (visual #3: visual #4 ═ 1:100 dilution) to each well and allowing to stand at room temperature for 90 minutes to allow anti-BrdU labeling peroxidase (peroxidase). The antibody conjugate was removed and washed three times with 200-. Finally, 100. mu.l of a substrate solution (TMB) was added to each well to develop the color of the reaction (visual #6), and after allowing to stand at room temperature for about 5 to 30 minutes, 25. mu.l of 1M H was added 2 SO 4 The reaction was terminated and the plates were shaken at 300rpm for about 1 minute. The absorbance at 450nm was measured by an enzyme-linked immunosorbent assay (ELISA reader) (BioTek, USA), and then statistical analysis was performed by Excel software.
The experimental results of the effect of the aquilaria sinensis extract on the promotion of the keratinocyte metabolism are shown in fig. 1, and the keratinocyte proliferation is remarkably increased after the aquilaria sinensis extract is treated, and is increased by about 10% compared with the keratinocyte proliferation result of a control group.
Example 3 the extract of Sagittaria frutescens of the present invention inhibits secretion of Interleukin-8 (IL-8) by ultraviolet irradiation
Since interleukin 8 (IL-8) is known as a cytokine and has a function of promoting the generation of inflammatory reaction, the present invention further examined the ability of the extract of Cassia nomama to inhibit the secretion of interleukin 8, which is caused by the irradiation of ultraviolet rays by keratinocytes. First, in a 24-well culture plate, 500. mu.l of the above culture solution was added to each well and 5X10 was placed 4 The keratinocytes were cultured at 37 ℃ for 24 hours. Removing the culture medium without disturbing the adherent cells, and treating the keratinocytes in four groups (n-3) with (1) a blank control group (mock) without adding the culture medium and without irradiating UVB, (2) a positive control group by adding the keratinocytes to the culture medium, and exposing the keratinocytes to light after culturing at 37 ℃ for 2 hours at 300mJ/cm 2 UVB, (3) and (4) for the experimental groups keratinocyte cells were added to a culture solution containing 0.125mg/mL or 0.0625mg/mL of the extract of Aquilaria sinensis of the present invention, respectively, and exposed to 300mJ/cm after culturing at 37 ℃ for 2 hours 2 After the four treatments, the keratinocytes were cultured at 37 ℃ for 24 hours, and 120. mu.l of the culture medium was collected after the lapse of time without disturbing the adherent cells.
The assay was then performed using an ELISA assay kit for human interleukin 8(IL-8/CXCL8) (available from R & D systems, USA). First, the capture antibody was diluted to the action concentration with phosphate buffer solution without antigen carrier and kept at 4 ℃, and immediately 100 μ l of the diluted capture antibody was added to each well of the 96-well plate to coat the bottom of the well of the 96-well plate with the capture antibody, then each well of the 96-well plate was washed three times with 200 μ l of washing solution (phosphate buffer solution containing 0.05% polyoxyethylene sorbitan monolaurate (tween 20)), impurities outside the capture antibody were removed, 300 μ l of blocking solution (phosphate buffer solution containing 1% Bovine Serum Albumin (BSA)) was added to each well to act at 37 ℃ for 3 hours to reduce the nonspecific binding of impurities to the capture antibody in the subsequent experiments, then each well of the 96-well plate was washed three times with 200 μ l of washing solution, and then 100 μ l of the culture solution of each group described in the above section was added, or dissolved in a standard substance containing 1% bovine serum albumin in phosphate buffer solution, and bound with the capture antibody at 37 ℃ for 2 hours, then, each well of the 96-well plate was washed three times with 200. mu.l of a washing solution, 100. mu.l of a detection antibody was added to each well of the 96-well plate, the capture antibody was detected at 37 ℃ for 2 hours, then washing each well of the 96-well culture plate three times by 200 mul of cleaning solution, adding 100 mul of horse radish peroxidase labeled Streptavidin (Streptavidin-HRP) to act for 20 minutes at room temperature, combining with the detection antibody, then, each well of the 96-well plate was washed three times with 200. mu.l of a washing solution, 100. mu.l of a coloring solution was added thereto, the mixture was allowed to act at room temperature for 20 minutes, 50. mu.l of a stop solution was further added thereto to stop the reaction, and finally, the absorbance at 450nm was measured by an enzyme immunoassay analyzer. Then statistical analysis is carried out by Excel software.
The experimental result of the water fragrant arris extract for reducing the skin inflammation reaction caused by the irradiation of ultraviolet light on the keratinocytes is shown in fig. 2, the secretion amount of the interleukin 8 in the skin keratinocytes of the positive control group is about 125pg/mL more than that of the blank control group, which shows that the skin keratinocytes irradiated with UVB can really promote the secretion of the interleukin 8, thereby causing the skin inflammation reaction. After the water fragrant arris extract is treated and irradiated with UVB, the secretion amount of interleukin 8 of keratinocytes is reduced by about 100pg/mL compared with that of a positive control group no matter the using amount is 0.125mg/mL or 0.0625mg/mL, and the result shows that the water fragrant arris extract can reduce the secretion of interleukin 8 induced by the irradiation of the UVB of the keratinocytes, effectively reduce the skin inflammation reaction caused by the irradiation of ultraviolet light of the keratinocytes and improve the skin protection.
Example 4 the effect of the extract of Aquilaria sinensis of the present invention to improve skin texture, pore size and reduce melanin content
In order to verify the efficacy of the water fragrant arris extract in skin changing capability, 1% of water fragrant arris essence added with 1% of water fragrant arris extract is prepared respectively; and an essence not containing the water fragrant arris extract as a control group essence, wherein the essence comprises water, jasminon, hexylene glycol, 1, 3-butylene glycol, xanthan gum, a thickening agent and triethanolamine, and the concentration of the water fragrant arris extract in one embodiment of the invention is preferably at least 0.05 mg/mL. Then, 8 subjects were recruited, each subject was washed every morning and evening, the control group essence and the essence containing the water fragrant prism extract of the present invention were applied to the Skin of the left and right half faces, and slightly rubbed with the finger abdomen to promote absorption, and Skin texture examination (Skin texture, pore size and melanin content were examined with Skin Analyzer TD) was performed before and 4 weeks after use, and the subjects were subjected to daily sun exposure during the test period.
The experimental results of the water fragrant edge extract for improving skin texture and pore size and reducing skin melanin content are shown in fig. 3-5, and after the essence containing the water fragrant edge extract is used, the skin texture can be improved by 20.8%, the skin pore size can be improved by 22%, and the skin melanin content can be reduced by 5% compared with the essence without the water fragrant edge extract.
In conclusion, the water fragrant arris extract extracted by using water, alcohol or an alcohol-water mixture as a solvent can effectively promote the proliferation of skin keratinocytes, and has the capabilities of promoting the rapid renewal of the skin keratinocytes and accelerating the removal of old cutin. The extract of the water fragrant arris can also reduce skin inflammation reaction caused by ultraviolet light irradiation of keratinocytes, and has the capability of improving skin protection. In addition, the water fragrant arris extract can also effectively improve skin texture and pore size and reduce melanin content, and has the capability of naturally changing skin and promoting skin cutin metabolism. Therefore, the water fragrant edge extract can be used for preparing a pharmaceutical composition for reducing skin inflammation reaction caused by ultraviolet light irradiation of keratinocytes and promoting skin cutin metabolism, and the composition can be prepared into powder, granules, liquid, jelly or paste and can be prepared into a medicine for being administered to an individual by oral administration, skin smearing and the like.
Claims (1)
1. Use of a water incense stick extract for preparing a composition for promoting skin cutin metabolism, wherein the water incense stick extract is obtained by extracting water incense sticks with a solvent, the solvent is water, the water incense stick extract is used for promoting keratinocyte proliferation and accelerating old cutin removal, and the liquid-solid ratio of the solvent to the water incense sticks is 5-20: 1-5, wherein the extraction step is carried out at 50-100 ℃, and the concentration of the water fragrant arris extract is at least 1.25 mg/mL.
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| CN107991159A (en) * | 2017-11-20 | 2018-05-04 | 珠海伊斯佳科技股份有限公司 | A kind of method by skin keratinocytes morphological assessment skin |
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| CN107991159A (en) * | 2017-11-20 | 2018-05-04 | 珠海伊斯佳科技股份有限公司 | A kind of method by skin keratinocytes morphological assessment skin |
Non-Patent Citations (3)
| Title |
|---|
| Remediation of Ultraviolet-Rays Induced Skin Damages by the Phytochemical Composition of Herbal Extracts;Madhulika Singh等;《International Journal of Pharmacognosy and Phytochemical Research》;20151231;第7卷(第6期);第1086-1093页 * |
| TRPV1在角质形成细胞释放炎症介质中的作用及药物的干预研究;黄瑾;《中国博士学位论文全文数据库 医药卫生科技辑》;20091015(第10期);第10-22页 * |
| Valencene from the Rhizomes of Cyperus rotundus Inhibits Skin Photoaging-Related Ion Channels and UV-Induced Melanogenesis in B16F10 Melanoma Cells;Joo Hyun Nam等;《Journal of Natural Products》;20161231;第79卷(第4期);第1091-1096页 * |
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