CN110179739A - A kind of efficiently compact weight-reducing liquid and its application method - Google Patents
A kind of efficiently compact weight-reducing liquid and its application method Download PDFInfo
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Abstract
The invention discloses a kind of efficiently compact weight-reducing liquid and its application methods, belong to compact weight loss field.Efficiently compact weight-reducing liquid is composed of the following components for this: carbomer, ethyl-para-hydroxybenzoate, collagen, elastin laminin, vitamin E, mannitol, the Stem Cell Activity factor, glycerol, deionized water.The invention has the benefit that introducing the cell repair factor in liquid component, increase compact fat-reducing effect, infection probability is reduced in preparation process, improve safety, subcutaneous damaged tissues can be repaired, it is compact to restore skin elasticity, a variety of obesities such as, honeycomb fat, edemous obesity, hormonal obesity fat to intractable there are better effects.
Description
Technical field
The present invention relates to compact weight loss field more particularly to a kind of efficiently compact weight-reducing liquid and its application methods.
Background technique
Currently, with reducing weight and beautifying features trend trend increasingly acutely, it is various to be answered about ways of preventing obesity and instrument
It transports and gives birth to, including liposuction, acupuncture, point massage and take slimming drugs etc., although the above method is it is possible that in the short time
Positive effect, still, the mechanism of action of above-mentioned weight-reducing is generally by promoting the loss of internal water quick to reach weight
The purpose of decline, or directly liposuction achieve the purpose that quick moulding, this that body is allowed to be in a new shape in a short time
The variation of state can cause many adverse reactions, and side effect is big；And slimming drugs be even more hundred evil and none is sharp, the meeting after taking slimming drugs
The normal operation mechanism for upsetting body leads to endocrine disturbance, and rebounds immediately after not taking slimming drugs, what is wasted money
On the basis of again the function of body can be made to decline, take for long periods of time and also result in inferior health.
With the development of modern biomedical material, weight-reducing of sunkening cord has evolved to the smaller minimally invasive rank of losing weight of sunkening cord of wound
Section.Minimally invasive weight-reducing of sunkening cord uses biological agent ingredient, and more mildly, without anesthesia, operation and notch, curative effect is very significant,
Securely and reliably, no pain, without any side effects, late result is good.
But currently marketed weight-reducing liquid majority fat-reducing effect is unobvious, may introduce bacterium in the process, virus etc. because
Element influences human health.
Summary of the invention
To solve the above-mentioned problems, the present invention provides the method for a kind of efficiently compact weight-reducing liquid and its preparation and application,
Cell factor is introduced in liquid component, increases compact fat-reducing effect, infection probability is reduced in preparation process, improves safety,
Subcutaneous damaged tissues can be repaired, recovery skin elasticity is compact, honeycomb obesity fat to intractable, edemous fat, hormone
A variety of obesities such as property obesity have better effects.
In order to achieve the above-mentioned object of the invention, the present invention provides a kind of efficiently compact weight-reducing liquid, the efficient compact weight-reducing liquids
It is composed of the following components:
Carbomer, ethyl-para-hydroxybenzoate, collagen, elastin laminin, vitamin E, mannitol, the Stem Cell Activity factor,
Glycerol, deionized water.
Efficiently compact weight-reducing liquid mass percentage composition is as follows for this:
Carbomer 0.08%-0.15%
Ethyl-para-hydroxybenzoate 0.05%-0.25%
Collagen 0.1%-0.2%
Elastin laminin 0.07%-0.2%
Vitamin E 0.1%-0.2%
Mannitol 0.05%-0.015%
Stem Cell Activity factor 0.02%-0.1%
Glycerol 10%-15%
Deionized water surplus.
The above ingredient is preferred are as follows:
Carbomer 0.09%-0.12%
Ethyl-para-hydroxybenzoate 0.09%-0.2%
Collagen 0.1%-0.2%
Elastin laminin 0.1%-0.15%
Vitamin E 0.1%-0.2%
Mannitol 0.05%-0.01%
Stem Cell Activity factor 0.02%-0.09%
Glycerol 10%-112%
Deionized water surplus.
Stem cell is introduced in mentioned component and plays preferable compact effect of weight reducing, and stem cell has self-replacation and orientation point
The ability of change, these active materials act on skin in the state of keeping activity, can effectively repair the epidermis of skin, corium
And subcutaneous tissue, and then be skin restore elasticity, it is smooth, have it is glossy, it is compact, it is seamless the effects of.
The present invention also provides a kind of preparation method for preparing above-mentioned efficiently compact weight-reducing liquid, preparation step is as follows:
1) it is spare to prepare the Stem Cell Activity factor；
2) by ethyl-para-hydroxybenzoate, vitamin E, glycerol disperses in deionized water, to be heated to 50-60 DEG C, stirring and dissolving；
3) liquid of step 2 is cooled to 40-45 DEG C, carbomer is added and continues to stir evenly；
4) liquid of step 3 is cooled to 25-30 DEG C, collagen and elastin laminin is added, be uniformly mixed；
5) step 4 liquid is subjected to logical oxygen processing with three oxygen machines；
6) cell active factor and the Stem Cell Activity factor is added in the liquid of step 5, stirred evenly, obtain efficient compact weight-reducing
Liquid.
The preparation step of the step 1 Stem Cell Activity factor is as follows:
1) human peripheral blood is equally handled with three oxygen machines, carries out centrifugally operated with centrifuge after allowing, obtains centrifugate
a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, puts into a centrifuge carry out centrifugally operated, obtains centrifugal liquid
b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case；
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and obtains the Stem Cell Activity factor.
In stem cell factor preparation process,
Three oxygen concentrations of step 1 be 11-42 ρ/μ g.ml-l, centrifuge parameters 2500-3000rmp, 10-15min,
The ratio of raffinate and NA cell separating liquid is 1:1, centrifuge parameters 1200-1600rmp in step 3, is centrifuged 10-15
In minute,
Incubator incubation time 24-30 hours in step 5, cultivation temperature are 35-37 DEG C,
Step 6 crusher parameter is broken 3-5s, stagnates 20-30s, is crushed 3-5 times.
Human blood in the preparation process of stem cell factor is his peripheral body liquid of human body or human body autologous peripheral blood.
Traditional stem cell preparation method, the cell factor low efficiency of cell secretion, and the cell in cell separation process
Also it is separated, the cell factor in cell is also separated, and causes to waste, in the production of the compact weight-reducing liquid of early period
There is the risk of infection of virus and bacterium in journey.1, the present invention introduces three oxygen methods in stem cell culture early period, so that cell exists
Secernment efficiency is improved during culture secrete cytokines, and the addition of ozone can effectively kill potential disease in three oxygen
Poison and bacterium reduce risk and improve safety；2, three oxygen methods also be joined in the manufacturing process of compact weight-reducing liquid, can also be had
Effect kills potential virus and bacterium, reduces risk and improves safety；3, rill crusher is introduced in stem cell preparation, it will be thin
Born of the same parents are broken, and also available effective utilization avoids wasting the juice factor in cell.
The present invention also provides a kind of application methods of above-mentioned efficiently compact weight-reducing liquid, specific steps are as follows:
1) first by a certain amount of compact weight-reducing liquid pouring into the sterility disinfected；
2) then soft object wire body is sandwiched in sterile disk with tweezers, it is spare takes out micropin；
3) soft object wire body is taken with tweezers, dips compact weight-reducing liquid, liquid is made to be evenly distributed on wire body surface；
4) soft object wire body is penetrated in micropin；
5) it carries out disinfection to the position for the operation that carry out sunkening cord；
6) finally with micropin by soft object wire body Buried body, the operation to compact weight-reducing of sunkening cord is completed.
Above-mentioned soft object wire body is by 10-15 parts of lecithin, 5-10 parts of NaTDC, 6-15 parts of soybean protein coating, brain phosphorus
15-20 parts of rouge, 30-64 parts of azelon rouge, mixing compacting weaves.
Straight thrust method or diagonal stabbing method are used when sunkening cord and operating, and diagonal stabbing method is used when using firm skin as main effect, with
Straight thrust method is used when weight-reducing is main effect.
The beneficial effects of the present invention are: introducing cytokine profiles in liquid component, compact fat-reducing effect is increased, is prepared
Infection probability is reduced in the process, improves safety.Three oxygen methods are introduced, the production efficiency of cell factor is improved, skin can be repaired
Lower damaged tissues, recovery skin elasticity is compact, and, honeycomb obesity fat to intractable, edemous obesity, hormonal obesity etc. are more
Kind obesity has better effects.
Detailed description of the invention
The influence of tri- oxygen concentration of Fig. 1 and breakage parameter to cell factor.
The picture that tri- oxygen concentration of Fig. 2 influences IFN-α；
Wherein: a comparative example 1, b embodiment 1, c embodiment 2, d embodiment 3, d embodiment 4.
Specific embodiment
In order to clarify the technical characteristics of the invention, being illustrated below by specific embodiment to this programme.
Comparative example 1
Comparative example 1 provides a kind of preparation method of Stem Cell Activity factor, and preparation step is as follows:
1) human peripheral blood is subjected to centrifugally operated centrifuge parameters 2800rmp, 10min with centrifuge, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 3s, stagnates 20s, is crushed 3 times, is obtained the Stem Cell Activity factor.
Embodiment 1
The embodiment of the invention provides a kind of preparation method of Stem Cell Activity factor, preparation step is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 11 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 3s, stagnates 20s, is crushed 3 times, is obtained the Stem Cell Activity factor.
Embodiment 2
The embodiment of the invention provides a kind of preparation method of Stem Cell Activity factor, preparation step is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 20 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 3s, stagnates 20s, is crushed 3 times, is obtained the Stem Cell Activity factor.
Embodiment 3
The embodiment of the invention provides a kind of preparation method of Stem Cell Activity factor, preparation step is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 3s, stagnates 20s, is crushed 3 times, is obtained the Stem Cell Activity factor.
Embodiment 4
The embodiment of the invention provides a kind of preparation method of Stem Cell Activity factor, preparation step is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 42 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 3s, stagnates 20s, is crushed 3 times, is obtained the Stem Cell Activity factor.
Comparative example 2
Comparative example 2 provides a kind of preparation method of Stem Cell Activity factor, and preparation step is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C, obtained the Stem Cell Activity factor
Embodiment 5
The embodiment of the invention provides a kind of preparation method of Stem Cell Activity factor, preparation step is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 5s, stagnates 30s, is crushed 5 times, is obtained the Stem Cell Activity factor.
It is carried out according to the above comparative example and embodiment, cell active factor secretes content experiment:
Experiment secretes IFN-α, the influence of INF- β, INF- γ using ELISPOT technology detection cell
The influence of 1 three oxygen concentration of table and breakage parameter to cell factor
Conclusion: by table 1, Fig. 1, Fig. 2 shows during preparing stem cell factor, and three oxygen methods and method of cell disruption is added,
The efficiency of cell-secretion factor can be improved.
Embodiment 6
The present invention provides a kind of efficiently compact weight-reducing liquids, and efficiently compact weight-reducing liquid mass percentage composition is as follows for this:
Carbomer 0.08%
Ethyl-para-hydroxybenzoate 0.05%
Collagen 0.1%
Elastin laminin 0.07%
Vitamin E 0.1%
Mannitol 0.05%
The Stem Cell Activity factor 0.02%
Glycerol 10%
Deionized water surplus
The preparation method of above-mentioned efficiently compact weight-reducing liquid, preparation step are as follows:
1) it is spare to prepare the Stem Cell Activity factor；
2) by ethyl-para-hydroxybenzoate, vitamin E, glycerol disperses in deionized water, to be heated to 50 DEG C, stirring and dissolving；
3) liquid of step 2 is cooled to 40 DEG C, carbomer is added and continues to stir evenly；
4) liquid of step 3 is cooled to 25 DEG C, collagen and elastin laminin is added, be uniformly mixed；
5) step 4 liquid is subjected to logical oxygen processing with three oxygen machines；
6) cell active factor and the Stem Cell Activity factor is added in the liquid of step 5, stirred evenly, obtain efficient compact weight-reducing
Liquid.
The preparation step of the Stem Cell Activity factor is as follows in above-mentioned steps 1:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 5s, stagnates 30s, is crushed 5 times, is obtained the Stem Cell Activity factor.
Embodiment 7
The present invention provides a kind of efficiently compact weight-reducing liquids, and efficiently compact weight-reducing liquid mass percentage composition is as follows for this:
Carbomer 0.15%
Ethyl-para-hydroxybenzoate 0.25%
Collagen 0.2%
Elastin laminin 0.07%-0.2%
Vitamin E 0.1%-0.2%
Mannitol 0.015%
The Stem Cell Activity factor 0.1%
Glycerol 15%
Deionized water surplus
The preparation method of above-mentioned efficiently compact weight-reducing liquid, preparation step are as follows:
1) it is spare to prepare the Stem Cell Activity factor；
2) by ethyl-para-hydroxybenzoate, vitamin E, glycerol disperses in deionized water, to be heated to 50 DEG C, stirring and dissolving；
3) liquid of step 2 is cooled to 40 DEG C, carbomer is added and continues to stir evenly；
4) liquid of step 3 is cooled to 25 DEG C, collagen and elastin laminin is added, be uniformly mixed；
5) step 4 liquid is subjected to logical oxygen processing with three oxygen machines；
6) cell active factor and the Stem Cell Activity factor is added in the liquid of step 5, stirred evenly, obtain efficient compact weight-reducing
Liquid.
The preparation step of the 1 Stem Cell Activity factor of above-mentioned steps is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 2800rmp, 10min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1500rmp is centrifuged 10 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 24 hours, cultivation temperature was 35 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 5s, stagnates 30s, is crushed 5 times, is obtained the Stem Cell Activity factor.
Embodiment 8
The present invention provides a kind of efficiently compact weight-reducing liquids, and efficiently compact weight-reducing liquid mass percentage composition is as follows for this:
Carbomer 0.08%
Ethyl-para-hydroxybenzoate 0.05%
Collagen 0.1%
Elastin laminin 0.07%
Vitamin E 0.1%
Mannitol 0.05%
The Stem Cell Activity factor 0.02%
Glycerol 10%
Deionized water surplus
The preparation method of above-mentioned efficiently compact weight-reducing liquid, preparation step are as follows:
1) it is spare to prepare the Stem Cell Activity factor；
2) by ethyl-para-hydroxybenzoate, vitamin E, glycerol disperses in deionized water, to be heated to 50 DEG C, stirring and dissolving；
3) liquid of step 2 is cooled to 45 DEG C, carbomer is added and continues to stir evenly；
4) liquid of step 3 is cooled to 30 DEG C, collagen and elastin laminin is added, be uniformly mixed；
5) step 4 liquid is subjected to logical oxygen processing with three oxygen machines；
6) cell active factor and the Stem Cell Activity factor is added in the liquid of step 5, stirred evenly, obtain efficient compact weight-reducing
Liquid.
The preparation step of the 1 Stem Cell Activity factor of above-mentioned steps is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 3000rmp, 15min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1600rmp is centrifuged 15 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 30 hours, cultivation temperature was 37 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 5s, stagnates 30s, is crushed 5 times, is obtained the Stem Cell Activity factor.
Embodiment 9
The embodiment of the invention provides a kind of efficiently compact weight-reducing liquids, and efficiently compact weight-reducing liquid mass percentage composition is as follows for this:
Carbomer 0.11%
Ethyl-para-hydroxybenzoate 0.15%
Collagen 0.15%
Elastin laminin 0.13%
Vitamin E 0.15%
Mannitol 0.05%
The Stem Cell Activity factor 0.6%
Glycerol 12%
Deionized water surplus
The preparation method of above-mentioned efficiently compact weight-reducing liquid, preparation step are as follows:
1) it is spare to prepare the Stem Cell Activity factor；
2) by ethyl-para-hydroxybenzoate, vitamin E, glycerol disperses in deionized water, is heated to 50 DEG C, stirring and dissolving；
3) liquid of step 2 is cooled to 45 DEG C, carbomer is added and continues to stir evenly；
4) liquid of step 3 is cooled to 30 DEG C, collagen and elastin laminin is added, be uniformly mixed；
5) step 4 liquid is subjected to logical oxygen processing with three oxygen machines；
6) cell active factor and the Stem Cell Activity factor is added in the liquid of step 5, stirred evenly, obtain efficient compact weight-reducing
Liquid.
The preparation step of the 1 Stem Cell Activity factor of above-mentioned steps is as follows:
1) human peripheral blood is equally handled with three oxygen machines, three oxygen concentrations be 36 ρ/μ g.ml-l, after allowing with centrifuge into
Row centrifugally operated centrifuge parameters 3000rmp, 15min, obtain centrifugate a；
2) centrifugate upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, the ratio of raffinate and NA cell separating liquid is 1:1, is put into
Centrifugally operated is carried out in centrifuge, centrifuge parameters 1600rmp is centrifuged 15 minutes, obtains centrifugal liquid b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case, incubator incubation time 30 hours, cultivation temperature was 37 DEG C,
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and crusher parameter is broken 5s, stagnates 30s, is crushed 5 times, is obtained the Stem Cell Activity factor.
Embodiment 10
Present embodiments provide a kind of application method of efficient compact weight-reducing liquid obtained such as embodiment 6-9, concrete operation step
It is as follows:
1) first by a certain amount of compact weight-reducing liquid pouring into the sterility disinfected；
2) then soft object wire body is sandwiched in sterile disk with tweezers, it is spare takes out micropin；
3) soft object wire body is taken with tweezers, dips compact weight-reducing liquid, liquid is made to be evenly distributed on wire body surface；
4) soft object wire body is penetrated in micropin；
5) it carries out disinfection to the position for the operation that carry out sunkening cord；
6) finally with micropin by soft object wire body Buried body, the operation to compact weight-reducing of sunkening cord is completed.
It is above-mentioned to sunken cord when operating using straight thrust method or diagonal stabbing method.Diagonal stabbing method is used when using firm skin as main effect,
Straight thrust method is used when main effect to lose weight.
Above-mentioned soft object wire body are as follows: 10-15 parts of lecithin, 5-10 parts of NaTDC, 6-15 parts of soybean protein coating, brain phosphorus
15-20 parts of rouge, 30-64 parts of azelon rouge, mixing compacting weaves.
1, body mass index test is carried out according to above embodiments 8 and embodiment 10:
Subject chooses: the age 20-30 years old, 11;Wherein male 5, female 6；
Age 30-40 years old, 15;Wherein male 7, female 8；
Age 40-45 years old, 4;Wherein male 2, female 2
Subject: according to the dietary standards and food-intake and feed frequency of test requirements document, with the accuracy of the result of guarantee test
And stability.
Treatment cycle: 20 days, the statistics of index is carried out daily.Final result is averaged.
Body mass index (BMI)=weight (kg)/[height (cm)]2
The pretherapy and post-treatment BMI comparison sheet of table 2
Conclusion: table 2 shows that testee's weight is substantially reduced, reaches effect using above-mentioned efficiently compact weight-reducing liquid treatment.
2, skin index variation is tested:
Subject chooses: the age 25-35 years old, 8;
Age 35-45 years old, 12;
Gender is women, because female skin is more sensitive to every index.
Table 3 treats each index situation of change of surrounding skin
Conclusion: table 3 shows to play the effect for more improving skin using above-mentioned efficiently compact weight-reducing liquid treatment etc..Wherein oil, water
Point, the indexs such as elasticity have clear improvement.
Technical characteristic of the present invention without description can realize that details are not described herein by or using the prior art, certainly,
The above description is not a limitation of the present invention, and the present invention is also not limited to the example above, the ordinary skill of the art
The variations, modifications, additions or substitutions that personnel are made within the essential scope of the present invention also should belong to protection model of the invention
It encloses.
Claims (9)
1. a kind of efficiently compact weight-reducing liquid, efficiently compact weight-reducing liquid is composed of the following components for this:
Carbomer, ethyl-para-hydroxybenzoate, collagen, elastin laminin, vitamin E, mannitol, the Stem Cell Activity factor,
Glycerol, deionized water.
2. a kind of efficiently compact weight-reducing liquid according to claim 1, the efficient compact weight-reducing liquid mass percentage composition is such as
Under:
Carbomer 0.08%-0.15%
Ethyl-para-hydroxybenzoate 0.05%-0.25%
Collagen 0.1%-0.2%
Elastin laminin 0.07%-0.2%
Vitamin E 0.1%-0.2%
Mannitol 0.05%-0.015%
Stem Cell Activity factor 0.02%-0.1%
Glycerol 10%-15%
Deionized water surplus.
3. a kind of preparation method for preparing efficiently compact weight-reducing liquid as claimed in claim 1 or 2, which is characterized in that the preparation side
Method the following steps are included:
1) the Stem Cell Activity factor is prepared, it is spare；
2) by ethyl-para-hydroxybenzoate, vitamin E, glycerol disperses in deionized water, to be heated to 50-60 DEG C, stirring and dissolving；
3) liquid of step 2 is cooled to 40-45 DEG C, carbomer is added and continues to stir evenly；
4) liquid of step 3 is cooled to 25-30 DEG C, collagen and elastin laminin is added, be uniformly mixed；
5) step 4 liquid is subjected to logical oxygen processing with three oxygen machines；
6) cell active factor and the Stem Cell Activity factor is added in the liquid of step 5, stirred evenly, obtain efficient compact weight-reducing
Liquid.
4. preparation method according to claim 3, which is characterized in that the preparation of the Stem Cell Activity factor in the step 1
Steps are as follows:
1) human peripheral blood is equally handled with three oxygen machines, then carries out centrifugally operated with centrifuge, obtains centrifugate
a；
2) centrifugate a upper plasma is drawn into centrifuge tube, it is spare, do not suck red blood cell；
3) NA cell separating liquid is added in remaining liq in step 2, puts into a centrifuge carry out centrifugally operated, obtains centrifugal liquid
b；
4) centrifugal liquid b supernatant liquid will be drawn, tunica albuginea layer is exposed, tunica albuginea layer liquid will be sucked out, does not suck lower layer's red blood cell；
5) by step 2, blood plasma dangles at the middle and upper levels, and the tunica albuginea layer in step 4 is added thereto, is transferred in culture bottle, training is put into
It supports and cultivates culture in case；
6) in step 5 culture bottle in blood plasma sucking centrifuge tube, centrifuge tube will be moved in the container for being placed with ice cube, clasmatosis is used
Machine is vacantly crushed, and obtains the Stem Cell Activity factor.
5. preparation method according to claim 3 or 4, which is characterized in that
Three oxygen concentrations of step 1 be 11-42 ρ/μ g.ml-l, centrifuge parameters 2500-3000rmp, 10-15min,
The ratio of raffinate and NA cell separating liquid is 1:1, centrifuge parameters 1200-1600rmp in step 3, is centrifuged 10-15
Minute,
Incubator incubation time 24-30 hours in step 5, cultivation temperature are 35-37 DEG C,
Step 6 crusher parameter is broken 3-5s, stagnates 20-30s, is crushed 3-5 times.
6. according to the described in any item preparation methods of claim 3-5, which is characterized in that the human peripheral blood in step 1 is
His peripheral body liquid of human body or human body autologous peripheral blood.
7. a kind of application method of efficient compact weight-reducing liquid described in -6 according to claim 1, which is characterized in that concrete operations step
It is rapid as follows:
1) first by a certain amount of compact weight-reducing liquid pouring into the sterility disinfected；
2) then soft object wire body is sandwiched in sterile disk with tweezers, it is spare takes out micropin；
3) soft object wire body is taken with tweezers, dips compact weight-reducing liquid, liquid is made to be evenly distributed on wire body surface；
4) soft object wire body is penetrated in micropin；
5) it carries out disinfection to the position for the operation that carry out sunkening cord；
6) finally with micropin by soft object wire body Buried body, the operation to compact weight-reducing of sunkening cord is completed.
8. the application method of efficient compact weight-reducing liquid according to claim 7, which is characterized in that the soft object wire body is by lecithin
10-15 parts of rouge, 5-10 parts of NaTDC, 6-15 parts of soybean protein coating, 15-20 parts of cephalin, azelon rouge 30-64
Part, mixing compacting weaves.
9. according to the described in any item efficiently compact weight-reducing liquid application methods of claim 7-8, which is characterized in that corresponding site buries
Using straight thrust method or diagonal stabbing method when line operates.
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| CN103519851A (en) * | 2012-07-04 | 2014-01-22 | 詹子昇 | Manufacturing method for sunken cord fatness reduction line |
| CN107541486A (en) * | 2017-08-23 | 2018-01-05 | 奥尔文（深圳）生物科技有限公司 | A kind of placenta Subaerial blue green algae active factors of beauty and skin care and its preparation and application |
| CN108186548A (en) * | 2018-03-19 | 2018-06-22 | 上海莱馥生命科学技术有限公司 | A kind of preparation method of the stem cell factor Essence with anti-aging effects |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103519851A (en) * | 2012-07-04 | 2014-01-22 | 詹子昇 | Manufacturing method for sunken cord fatness reduction line |
| CN107541486A (en) * | 2017-08-23 | 2018-01-05 | 奥尔文（深圳）生物科技有限公司 | A kind of placenta Subaerial blue green algae active factors of beauty and skin care and its preparation and application |
| CN108186548A (en) * | 2018-03-19 | 2018-06-22 | 上海莱馥生命科学技术有限公司 | A kind of preparation method of the stem cell factor Essence with anti-aging effects |
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