CN110179706A - A kind of Lonicera Japonica extracting solution and preparation method thereof with whitening function - Google Patents
A kind of Lonicera Japonica extracting solution and preparation method thereof with whitening function Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The invention discloses a kind of Lonicera Japonica extracting solution and preparation method thereof with whitening function, it is related to daily chemical products technical field.It is made that it by organic diol-water two part solvent system is total to molten ultrasonic method, its preparation step are as follows: Lonicera Japonica is dissolved in organic diol-water in the mixed solvent, by the mixed liquor of formation in 10 DEG C of -25 DEG C of standing 5-24h, by mixed liquor at 50 DEG C -80 DEG C ultrasound 1-10h, mixed liquor is cooled to 5 DEG C -25 DEG C, mixed liquor is obtained into Lonicera Japonica extracting solution by plate-frame filtering.Present invention process is simple, and preparation condition is mild, will not destroy bioactivity, and having improves skin microcirculation, inhibits tyrosinase activity and oxidation resistant effect, can be effectively improved skin complexion, achieve the effect that whitening, it is easy to accomplish industrialized production.
Description
Technical field
The present invention relates to daily chemical products technical fields, and in particular to a kind of Lonicera Japonica extracting solution with whitening function and
Preparation method.
Background technique
Lonicera Japonica, also known as honeysuckle are a kind of conventional Chinese medicines with long history, and effect is mainly clearing heat and detoxicating, main
Control warm disease heating, toxic-heat and blood stasis, ulcer serious case of furuncle etc.；It contains the pharmacological components such as chlorogenic acid, flavonoids, cyanidenon glycosides,
Its chemical component, pharmacological action and its safety etc. are by extensive concern.Market two flower prods are chiefly used in it is medicinal, for
Its in skin care item using less, and in the researches of skin-whitening anti-oxidation field.
The problem of skin-whitening is always numerous people seeking beauties eternal concern, research and development, safety and effect of whitening product
Property evaluation become Cosmetic Manufacture and consumer group's focus of attention.From the mechanism of melanin production, tyrosinase is
The key enzyme of organism synthesis of melanin, the depth of human epidermal pigment and pigmentation disorder etc. with the table of tyrosinase
Up to in vivo activity it is related, to tyrosinase activity inhibit be conducive to whitening effect.But many whitening formulas in the market
In the whitening composition that contains be inorganic or organic substance, wherein the whitening effect of inorganic matter functions only as the purpose covered, essence
On the effect of skin-whitening is not achieved；And organic whitening material is then set about from the mechanism of melanin production, blocks melanin raw
At to reach whitening effect.Therefore it provides a kind of natural plant whitening component that can effectively make skin-whitening is with important
Meaning.
Summary of the invention
In view of the shortcomings of the prior art, object of the present invention is to be to provide a kind of Lonicera Japonica with whitening function to mention
Liquid and preparation method thereof is taken, simple process, preparation condition is mild, will not destroy bioactivity, it can be effectively improved skin complexion,
Achieve the effect that whitening, it is easy to accomplish industrialized production.
To achieve the goals above, the present invention is to realize by the following technical solutions: a kind of with whitening function
Lonicera Japonica extracting solution, is total to that molten ultrasonic method is made, and the active component in extracting solution is total by organic diol-water two part solvent system
The weight percentage of phenolic acid is at least 55%.
Preferably, the weight percentage of active component total phenolics is in 55%-80% in the Lonicera Japonica extracting solution.
A kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows: (1) be dissolved in Lonicera Japonica
Machine dihydric alcohol-water in the mixed solvent；
(2) mixed liquor for forming step (1) is in 10 DEG C of -25 DEG C of standing 5-24h；
(3) mixed liquor for forming step (2) ultrasound 1-10h at 50 DEG C -80 DEG C；
(4) mixed liquor that step (3) are formed is cooled to 5 DEG C -25 DEG C；
(5) mixed liquor that step (4) are formed is obtained into Lonicera Japonica extracting solution by plate-frame filtering.
Preferably, the concentration of Lonicera Japonica is 0.01mg/mL-100mg/mL in the step (1).
Preferably, in the step (1) organic diol in the mixed solvent concentration be 0.1mL/mL-10mL/mL.
Preferably, the volume ratio of organic diol and water is (0.1-10) in the step (1): 1.
Preferably, in the step (1) organic diol be propylene glycol, butanediol, pentanediol, hexylene glycol, nonanediol,
At least one of decanediol.
Beneficial effects of the present invention: (1) the effective component total phenolics in Lonicera Japonica extracting solution, which have, improves skin microcirculation, suppression
Tyrosinase activity processed and oxidation resistant effect, can be effectively improved skin complexion, achieve the effect that whitening.
(2) the Lonicera Japonica extracting solution is total to molten ultrasonic extraction, the technique item of preparation method by organic diol-water two part solvent
Part is very mild, will not destroy the structure of Lonicera Japonica itself, it is thus possible to guarantee the bioactivity of Lonicera Japonica.
(3) simple process, it is easy to accomplish industrialized production, large-scale application have a extensive future.
Detailed description of the invention
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments；
Fig. 1 is the liquid chromatogram of two flower extracts in the embodiment of the present invention 1；
Fig. 2 is two flower extracts in the embodiment of the present invention 1 to the elimination effect comparison diagram of DPPH free radical.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
Referring to Fig.1-2, present embodiment uses a kind of following technical scheme: Lonicera Japonica extracting solution with whitening function
And preparation method thereof, it is total to that molten ultrasonic method is made, and can effectively improve Lonicera Japonica has by organic diol-water two part solvent system
The extraction of ingredient is imitated, the preparation method mild condition will not destroy the bioactivity of Lonicera Japonica, the wherein work in Lonicera Japonica extracting solution
The weight percentage of property component total phenolics is at least 55%.
It is worth noting that, the weight percentage of active component total phenolics is in 55%- in the Lonicera Japonica extracting solution
80%.
A kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows: (1) be dissolved in Lonicera Japonica
Machine dihydric alcohol-water in the mixed solvent；
(2) mixed liquor for forming step (1) is in 10 DEG C of -25 DEG C of standing 5-24h；
(3) mixed liquor for forming step (2) ultrasound 1-10h at 50 DEG C -80 DEG C；
(4) mixed liquor that step (3) are formed is cooled to 5 DEG C -25 DEG C；
(5) mixed liquor that step (4) are formed is obtained into Lonicera Japonica extracting solution by plate-frame filtering.
It is worth noting that, the concentration of Lonicera Japonica is 0.01mg/mL-100mg/mL in the step (1), organic diol exists
The concentration of in the mixed solvent is 0.1mL/mL-10mL/mL；The volume ratio of organic diol and water is (0.1-10): 1；Organic two
First alcohol uses propylene glycol, butanediol, pentanediol, hexylene glycol, nonanediol, at least one of decanediol.
Embodiment 1: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,2-PD and water are formed then is added thereto,
1,2-PD is 1mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 10mg/mL；
(2) by the conical flask equipped with mixed liquor in 15 DEG C of standing 10h；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 50 DEG C, ultrasonic 4h；
(4) after the conical flask equipped with mixed liquor being cooled to 10 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid.
The present embodiment detects two flower extract of gained by liquid chromatograph, testing result such as Fig. 1；To above-mentioned gained two
Flower extract carries out antioxidant activity evaluation, by two flower extracts and vitamin C, alpha-arbutin, glabridin respectively to DPPH
Free radical scavenging activity measurement experiment, as a result as shown in Figure 2.
Embodiment 2: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,2- butanediol and water are formed then is added thereto,
1,2- butanediol is 0.3mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 30mg/mL；
(2) by the conical flask equipped with mixed liquor in 20 DEG C of standing 20h；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 60 DEG C, ultrasonic 6h；
(4) after the conical flask equipped with mixed liquor being cooled to 20 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid.
The present embodiment detects above-mentioned two flower extract of gained by liquid chromatograph, the inspection of testing result and embodiment 1
It is close to survey result；Tyrosinase inhibitory activity evaluation is carried out to above-mentioned two flower extract of gained, by two flower extracts and vitamin
C, alpha-arbutin, glabridin are respectively to monophenolase measurement experiment, and the results are shown in Table 1 for 503nhibiting concentration.
1 whitening component of table is to tyrosinase monophenolase depression effect
Embodiment 3: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,6- hexylene glycol and water are formed then is added thereto,
1,6- hexylene glycol is 2mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 50mg/mL；
(2) conical flask equipped with mixed liquor is stood for 24 hours at 25 DEG C；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 80 DEG C, ultrasonic 8h；
(4) after the conical flask equipped with mixed liquor being cooled to 25 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid, and gained liquid is sealed in 0-15 DEG C.
The present embodiment detects two flower extract of gained by liquid chromatograph, the detection knot of testing result and embodiment 1
Fruit is close；Tyrosinase inhibitory activity evaluation is carried out to above-mentioned two flower extract of gained.By two flower extracts and vitamin C, α-
Arbutin, glabridin respectively test bis-phenol enzymatic determination, and the results are shown in Table 2 for 503nhibiting concentration.
2 whitening component of table is to tyrosinase bis-phenol enzyme depression effect
Embodiment 4: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,3-PD and water are formed then is added thereto,
1,3-propylene glycol is 0.5mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 100mg/mL；
(2) by the conical flask equipped with mixed liquor in 15 DEG C of standing 20h；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 50 DEG C, ultrasonic 10h；
(4) after the conical flask equipped with mixed liquor being cooled to 20 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid, and gained liquid is sealed in 0-15 DEG C.
The present embodiment detects two flower extract of gained by liquid chromatograph, the detection knot of testing result and embodiment 1
Fruit is close.
Embodiment 5: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,9- nonanediol and water are formed then is added thereto,
1,9-nonanediol is 3mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 20mg/mL；
(2) by the conical flask equipped with mixed liquor in 20 DEG C of standing 14h；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 65 DEG C, ultrasonic 7h；
(4) after the conical flask equipped with mixed liquor being cooled to 20 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid, and gained liquid is sealed in 0-15 DEG C.
The present embodiment detects two flower extract of gained by liquid chromatograph, the detection knot of testing result and embodiment 1
Fruit is close.
Embodiment 6: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,2- pentanediol and water are formed then is added thereto,
1,2-pentanediol is 2mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 60mg/mL；
(2) by the conical flask equipped with mixed liquor in 15 DEG C of standing 22h；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 60 DEG C, ultrasonic 8h；
(4) after the conical flask equipped with mixed liquor being cooled to 15 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid, and gained liquid is sealed in 0-15 DEG C.
The present embodiment detects two flower extract of gained by liquid chromatograph, the detection knot of testing result and embodiment 1
Fruit is close.
Embodiment 7: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 1,10- decanediol and water are formed then is added thereto,
1,10-decanediol is 1mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 50mg/mL；
(2) by the conical flask equipped with mixed liquor in 20 DEG C of standing 18h；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 75 DEG C, ultrasonic 10h；
(4) after the conical flask equipped with mixed liquor being cooled to 20 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid, and gained liquid is sealed in 0-15 DEG C.
The present embodiment detects two flower extract of gained by liquid chromatograph, the detection knot of testing result and embodiment 1
Fruit is close.
Embodiment 8: a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, preparation step are as follows:
(1) Lonicera Japonica is added in 250mL conical flask, the mixed liquor that 2,3-butanediol and water are formed then is added thereto,
2,3-butanediol is 1.5mL/mL in the concentration of in the mixed solvent, and concentration of the Lonicera Japonica in mixed liquor is 70mg/mL；
(2) conical flask equipped with mixed liquor is stood for 24 hours at 15 DEG C；
(3) conical flask equipped with mixed liquor is put on ultrasonic cleaning machine, bath temperature is 65 DEG C, ultrasonic 3h；
(4) after the conical flask equipped with mixed liquor being cooled to 20 DEG C, decompression is filtered, and obtaining filtrate is that Lonicera Japonica is extracted
Liquid, and gained liquid is sealed in 0-15 DEG C.
The present embodiment detects two flower extract of gained by liquid chromatograph, the detection knot of testing result and embodiment 1
Fruit is close.
Embodiment 9:
(1) the present embodiment carries out diphenyl trinitrophenyl-hydrazine to the Lonicera Japonica extracting solution of the present invention with whitening function
(DPPH) measurement experiment of the Scavenging activity of free radical.
Lonicera Japonica extracting solution and vitamin C, alpha-arbutin, glabridin prepared by Example 1 is matched with dehydrated alcohol respectively
For the solution of various concentration processed as whitening prepare liquid (10 μ g/mL-500 μ g/mL), DPPH is configured to 0.2mmol/ with dehydrated alcohol
The solution of L.It takes the whitening prepare liquid of 2mL to mix with the DPPA of 2mL respectively, in 25 DEG C of standing 30min of room temperature, uses ultraviolet-visible
Spectrophotometer measures absorbance A of the system at 517nm respectivelyi、Aj、A0, every group of measurement is averaged three times, and uses following formula
Calculate the clearance rate of DPPH free radical:
Wherein, A0The absorbance value of 2mL dehydrated alcohol, A are added for 2mLDPPH solutioni2mL is added not for 2mLDPPH solution
With the absorbance value of the whitening prepare liquid of concentration, AjThe extinction of the whitening prepare liquid of 2mL various concentration is added for 2mL dehydrated alcohol
Angle value.
Lonicera Japonica extracting solution prepared by embodiment 1 to the clearance rate result of DPPH free radical as shown in Fig. 2, as can be seen from Figure 2, it is real
The Lonicera Japonica extracting solution for applying the preparation of example 1 has good scavenging ability of DPPH free radical, than vitamin C, alpha-arbutin, glabridin
Clearance rate it is higher, illustrate embodiment 1 prepare Lonicera Japonica extracting solution have good antioxygenic property.
(2) the present embodiment carries out the work of tyrosinase monophenolase to the Lonicera Japonica extracting solution of the present invention with whitening function
The measurement experiment of property rejection ability.
Using l-tyrosine as substrate, whitening compound is probed into the inhibiting effect of tyrosinase monophenolase.Prepare pH=
6.8, the PBS buffer solution (phosphate buffered saline solution) of 0.5mol/L.
1. Lonicera Japonica extracting solution prepared by embodiment 2 and vitamin C, alpha-arbutin, glabridin are dissolved and DMSO respectively
In, use PBS buffer solution to be diluted to various concentration as whitening prepare liquid, DMSO concentration is 3%；
2. tyrosinase is formulated as the solution that enzyme activity is 50U/mL with PBS buffer solution；
3. using PBS buffer solution to be formulated as solution that concentration is 6mmol/L, 0.5mmol/L respectively the bottom of as l-tyrosine
Object solution；
4. in test tube, sequentially adding above-mentioned matched solution: l-tyrosine solution, whitening prepare liquid, PBS buffer solution, in
37 DEG C of water-bath 10min；
5. tyrosinase solution is added into the test tube equipped with the mixed liquor of above-mentioned steps 4., in 37 DEG C of water-bath 30min；
6. by above-mentioned steps, 5. resulting mixed liquor is transferred in cuvette, is measured at four groups of whitening system 475nm respectively
Absorbance.Every group of measurement is averaged three times, and calculates different whitening prepare liquids to the shadow of tyrosinase vigor with following formula
It rings:
Wherein, A0The absorbance value of live body system, A when for containing l-tyrosine, tyrosinase, whitening prepare liquid1For containing
The absorbance value of live body system when l-tyrosine, tyrosinase.
7. after the enzyme activity for calculating above-mentioned different solutions, calculating the half inhibiting rate IC of different whitening prepare liquids50.Knot
Fruit is as shown in table 1.
As known from Table 1,503nhibiting concentration IC of the Lonicera Japonica extracting solution that prepared by embodiment 2 to tyrosinase monophenolase50=
0.00137mmol/L, hence it is evident that be lower than vitamin C (IC50=0.00282mmol/L), alpha-arbutin (IC50=3.94mmol/L)
With glabridin (IC50=0.0051mmol/L), the Lonicera Japonica extracting solution for illustrating prepared by embodiment 2 has fine inhibition to tyrosinase
Effect.
(3) the present embodiment carries out the work of tyrosinase bis-phenol enzyme to the Lonicera Japonica extracting solution of the present invention with whitening function
The measurement experiment of property rejection ability.
Using L-3,4 dihydroxyphenylalanine as substrate, whitening compound is probed into the inhibiting effect of tyrosinase bis-phenol enzyme.Preparation pH=6.8,
The PBS buffer solution (phosphate buffered saline solution) of 0.5mol/L.
1. Lonicera Japonica extracting solution prepared by embodiment 3 and vitamin C, alpha-arbutin, glabridin are dissolved and DMSO respectively
In, use PBS buffer solution to be diluted to various concentration as whitening prepare liquid, DMSO concentration is 3%；
2. tyrosinase is formulated as the solution that enzyme activity is 50U/mL with PBS buffer solution；
3. using PBS buffer solution to be formulated as solution that concentration is 6mmol/L, 0.5mmol/L respectively as substrate L-3,4 dihydroxyphenylalanine
Solution；
4. in test tube, sequentially adding above-mentioned matched solution: L-3,4 dihydroxyphenylalanine solution, whitening prepare liquid, PBS buffer solution, in 37
DEG C water-bath 10min；
5. tyrosinase solution is added into the test tube equipped with the mixed liquor of above-mentioned steps 4., in 37 DEG C of water-bath 30min；
6. by above-mentioned steps, 5. resulting mixed liquor is transferred in cuvette, is measured at four groups of whitening system 475nm respectively
Absorbance.Every group of measurement is averaged three times, and calculates different whitening prepare liquids to the shadow of tyrosinase vigor with following formula
It rings:
Wherein, A0The absorbance value of live body system, A when for containing L-3,4 dihydroxyphenylalanine, tyrosinase, whitening prepare liquid1To contain L-
The absorbance value of live body system when DOPA, tyrosinase.
7. after the enzyme activity for calculating above-mentioned different solutions, calculating the half inhibiting rate IC of different whitening prepare liquids50, knot
Fruit is as shown in table 2.
As known from Table 2,503nhibiting concentration IC of the Lonicera Japonica extracting solution that prepared by embodiment 3 to tyrosinase bis-phenol enzyme50=
0.0322mmol/L, hence it is evident that be lower than Symwhite-337 (IC50=0.0475mmol/L) and glabridin (IC50=
0.0076mmol/L), 503nhibiting concentration IC of the alpha-arbutin to tyrosinase diphenolase50For negative value, illustrate alpha-arbutin to junket
Propylhomoserin enzyme has activation, while the Lonicera Japonica extracting solution for illustrating prepared by embodiment 2 has fine inhibiting effect to tyrosinase.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (7)
1. a kind of Lonicera Japonica extracting solution with whitening function, which is characterized in that be total to by organic diol-water two part solvent system
Molten ultrasonic method is made, and the weight percentage of the active component total phenolics in extracting solution is at least 55%.
2. a kind of Lonicera Japonica extracting solution with whitening function according to claim 1, which is characterized in that the Lonicera Japonica mentions
Take the weight percentage of active component total phenolics in liquid in 55%-80%.
3. a kind of preparation method of the Lonicera Japonica extracting solution with whitening function, which is characterized in that its preparation step are as follows: (1) by two
Flower is dissolved in organic diol-water in the mixed solvent；
(2) mixed liquor for forming step (1) is in 10 DEG C of -25 DEG C of standing 5-24h；
(3) mixed liquor for forming step (2) ultrasound 1-10h at 50 DEG C -80 DEG C；
(4) mixed liquor that step (3) are formed is cooled to 5 DEG C -25 DEG C；
(5) mixed liquor that step (4) are formed is obtained into Lonicera Japonica extracting solution by plate-frame filtering.
4. a kind of preparation method of Lonicera Japonica extracting solution with whitening function according to claim 3, which is characterized in that institute
The concentration for stating Lonicera Japonica in step (1) is 0.01 mg/mL-100mg/mL.
5. a kind of preparation method of Lonicera Japonica extracting solution with whitening function according to claim 3, which is characterized in that institute
It is 0.1mL/mL-10mL/mL that organic diol in step (1), which is stated, in the concentration of in the mixed solvent.
6. a kind of preparation method of Lonicera Japonica extracting solution with whitening function according to claim 3, which is characterized in that institute
Stating the volume ratio of organic diol and water in step (1) is (0.1-10): 1.
7. a kind of preparation method of Lonicera Japonica extracting solution with whitening function according to claim 3, which is characterized in that institute
Stating organic diol in step (1) is propylene glycol, butanediol, pentanediol, hexylene glycol, nonanediol, at least one of decanediol.
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