CN110172390A - A kind of high motile sperm screening micro fluidic device - Google Patents
A kind of high motile sperm screening micro fluidic device Download PDFInfo
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- CN110172390A CN110172390A CN201910465724.6A CN201910465724A CN110172390A CN 110172390 A CN110172390 A CN 110172390A CN 201910465724 A CN201910465724 A CN 201910465724A CN 110172390 A CN110172390 A CN 110172390A
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- sperm
- inner cavity
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention discloses a kind of high motile sperms to screen micro fluidic device, including cylinder, the inner cavity of the cylinder is separated into inside and outside two chambers by thin layer annular wall, and inner cavity chamber and exocoel are concentric structure, full sperm nutrient solution is set by inner cavity chamber, exocoel is equipped with semen sample, so that semen sample is just covered inner cavity chamber upper surface, the present invention relates to field of biotechnology.The high motile sperm screens micro fluidic device, using sperm swim-up and along interface swimming characteristic, avoid existing assisted reproductive technology potential for adverse effects of the various operations such as external centrifugation, washing to sperm in the process, structure setting compact and reasonable, and high motile sperm can effectively be concentrated, high motile sperm needed for can get assisted reproductive technology, to greatly improve success rate in vitro fertilization, it is applied widely, it is applicable not only to the reproductive center of for example each medical institutions of human reproduction, and it is suitable for animal breeding, this device can be used in human reproduction and animal breeding.
Description
Technical field
The present invention relates to field of biotechnology, specially a kind of high motile sperm screens micro fluidic device.
Background technique
Infertile disease incidence is about 15%-20% in world wide, and has cumulative year after year trend, pre- according to WHO
It surveys, in the near future, healthy reproduction problem will threaten the third-largest of human health after cardiovascular and cerebrovascular disease and tumour
Disease, the increase of infertile disease incidence have pushed the development of assisted reproductive technology, first generation test-tube baby's skill significantly
Art --- IVF-ET Technique (IVF-ET) has been brought since 1978 carry out to many infertility Mr. and Mrs
Gospel；Second generation tube-test baby techniques --- intracytoplasmic sperm injection (ICSI) technology and third-generation technology --- are planted
Enter the further abundant and perfect assisted reproductive technology of preceding genetic diagnosis (PGD), nevertheless, assisted reproductive technology mistake
It is many in journey to operate the damage that may cause sperm, embryo's agensis or miscarriage are eventually led to, this is also to lead to test tube baby
Youngster's technique successful rate be difficult to be more than 50% one of the major reasons, these operations haveing damage include: the centrifugation after sperm swim-up
The influence etc. of certain ingredients in operation, density gradient centrifugation operation and separating liquid, and the influence that avoid these from operating, to height
The screening of motile sperm should avoid as far as possible centrifugally operated and play inspissation.
Spermatogenesis and it is mature abnormal it is most common show as oligospermatism and asthénospermie, but these patients always have
The normal sperm of part vigor, laboratory commonly separate high-quality sperm technology be upper reaches method and density-gradient centrifugation method, this
A little operations will cause damage to high-quality sperm, and can reach Natural Selection vigor just by aperture depth difference on micro-fluidic chip
The purpose of Chang Jingzi；The high motile sperm filtered out passes through centrifugal concentrating, still can cause to damage to sperm, and high motile sperm
When crossing separate layer another small volume chamber of arrival, nature concentration can be played, centrifugally operated is avoided, therefore pass through this miniflow
Control device can screen high motile sperm and is concentrated, and the sperm filtered out can directly carry out (IVF) or egg cell in vitro fertilization
Intracytoplasmic sperm injection (ICSI) in matter.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, the present invention provides a kind of high motile sperms to screen micro fluidic device, solves existing
Certain in centrifugally operated, density gradient centrifugation operation and separating liquid after not being avoided that sperm swim-up in some sperm screening processes
The problem of influence operation of a little ingredients causes to damage, not can guarantee spermatozoon activity to sperm.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs: a kind of high motile sperm screening miniflow
Control device, including cylinder, the inner cavity of the cylinder is separated into inside and outside two chambers by thin layer annular wall, and inner cavity chamber and outside
Chamber is concentric structure, and structure of the invention setting compact and reasonable is avoided using sperm swim-up and along interface swimming characteristic
The various potential for adverse effects operated to sperm such as external centrifugation, washing, and high motile sperm can effectively be concentrated, utilize this hair
High motile sperm needed for bright device can get assisted reproductive technology, can greatly improve success rate in vitro fertilization, the invention
Device is applicable not only to the reproductive center of for example each medical institutions of human reproduction, and is suitable for animal breeding.
Preferably, full sperm nutrient solution is set by the inner cavity chamber, and the exocoel is equipped with semen sample, and makes semen sample
Just cover inner cavity chamber upper surface, upstream sperm reach exocoel top after along interface swimming to inner cavity chamber, will finally converge in
Inner cavity chamber.
Preferably, the inner cavity chamber radius is 2.5mm, and depth 20mm, the net radius of exocoel annular is 2mm, deep
Degree is 20mm, and the thin layer annular wall between the interior exocoel is highly 20mm with a thickness of 0.5mm, simple and reasonable, easily
In realization.
Preferably, the sperm nutrient solution is one voluntarily prepared in sperm nutrient solution, commercialization Earle ' s liquid or BWW liquid
Kind or a variety of combinations, and male patient of the semen sample from infertile couples.
Preferably, the sperm nutrient solution is composed of the following components in voluntarily process for preparation: NaCl 0.721g,
MgSO4·7H2O 0.247g, fructose 1g, Na2HPO4 3.581g、KH2PO40.136g and pure water 1000ml, sperm nutrition
Liquid component is reasonable, and the vigor of sperm can be effectively ensured, and effect is good.
Preferably, a kind of preparation method of high motile sperm screening micro fluidic device, specifically includes the following steps:
S1, Ni-based, monocrystalline silicon or glass mold plungers are prepared by micro fluidic device design drawing；
S2, polymetylmethacrylate sheet material is cut into small pieces by formpiston size, cleaned, drying；
S3, by step S2, treated that polymetylmethacrylate sheet material is loaded to formpiston, compresses；
S4, PMMA is sealed 12-15 minutes under heating, pressurization using tablet press machine, water flowing is cooled to room temperature；
S5, by step S4, treated that formpiston carries out demoulding processing, and the micro fluidic device with channel can be obtained.
A kind of preparation method of high motile sperm screening micro fluidic device of the present invention, method is reasonable, using just
Victory, is suitble to large-scale industrial production, and production cost is low.
Preferably, a kind of working method of high motile sperm screening micro fluidic device, specifically includes the following steps:
T1, it after rinsing inside and outside chamber with deionized water, is dried in 37 DEG C of baking ovens；
T2, take sperm nutrient solution filling with sample injector to inner cavity chamber, filling total amount is 400 μ l；
T3, take the semen sample of mixing filling with sample injector to exocoel, filling total amount is 1ml；
T4, the semen sample for taking sperm nutrient solution and the other three to mix with method, it is filling to other three interior exocoels respectively
Room；
T5, with sealing film capping apparatus surface, that is, cover 4 interior exocoels opening, device is then horizontally placed at 37
In DEG C wet box；
T6, semen sample in exocoel is absorbed after 1 hour, and collect the sperm in inner cavity chamber containing high motile sperm and seek
Nutrient solution；
T7, this sperm nutrient solution containing high motile sperm can be directly used for it is in vitro fertilization or therefrom picking sperm carry out ovum
Intracytoplasmic sperm injection in cytoplasm.
A kind of working method of high motile sperm screening micro fluidic device of the present invention, method is reasonable, using side
Just, it can be enriched with the sperm that form is normal, DNA fragmentation index (DFI) is low, vigor is good in vitro and be used for IVF or ICSI.
(3) beneficial effect
The present invention provides a kind of high motile sperms to screen micro fluidic device.Have compared with prior art following beneficial
Effect:
(1), the high motile sperm screens micro fluidic device, by avoiding using sperm swim-up and along interface swimming characteristic
Potential for adverse effects of the various operations such as external centrifugation, washing to sperm, structure setting during existing assisted reproductive technology
Compact and reasonable, and high motile sperm can effectively be concentrated.
(2), the high motile sperm screens micro fluidic device, by utilizing the difference of chamber volume inside and outside concentric structure,
It is thin for (IVF) in vitro fertilization or ovum that the sperm that form is normal, DNA fragmentation index (DFI) is low, vigor is good can be effectively enriched with
Intracytoplasmic sperm injection (ICSI), high motile sperm needed for can get assisted reproductive technology are in vitro fertilization to greatly improve
Success rate.
(3), the high motile sperm screens micro fluidic device, applied widely, is applicable not only to for example each medical treatment of human reproduction
The reproductive center of mechanism, and it is suitable for animal breeding, this device can be used in human reproduction and animal breeding.
Detailed description of the invention
Fig. 1 is the top view of micro fluidic device of the present invention；
Fig. 2 is the front view inside micro fluidic device of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its
His embodiment, shall fall within the protection scope of the present invention.
The embodiment of the present invention provides three kinds of technical solutions referring to FIG. 1-2: a kind of micro-fluidic dress of high motile sperm screening
It sets, specifically includes following embodiment:
Embodiment 1
A kind of high motile sperm screening micro fluidic device, including cylinder, the inner cavity of the cylinder pass through thin layer annular wall point
Inside and outside two chambers are divided into, and inner cavity chamber and exocoel are concentric structure, full sperm nutrient solution, the exocoel are set by inner cavity chamber
Room is equipped with semen sample, and semen sample is made just to cover inner cavity chamber upper surface, and inner cavity chamber radius is 2.5mm, and depth is
20mm, the net radius of exocoel annular are 2mm, depth 20mm, the thin layer annular wall between the interior exocoel with a thickness of
0.5mm is highly 20mm, and sperm nutrient solution is voluntarily to prepare sperm nutrient solution, and male of the semen sample from infertile couples
Patient, sperm nutrient solution are composed of the following components in voluntarily process for preparation: NaCl 0.721g, MgSO4·7H2O
0.247g, fructose 1g, Na2HPO4 3.581g、KH2PO40.136g and pure water 1000ml.
A kind of preparation method of high motile sperm screening micro fluidic device, specifically includes the following steps:
S1, Ni-based, monocrystalline silicon or glass mold plungers are prepared by micro fluidic device design drawing；
S2, polymetylmethacrylate sheet material is cut into small pieces by formpiston size, cleaned, drying；
S3, by step S2, treated that polymetylmethacrylate sheet material is loaded to formpiston, compresses；
S4, PMMA is sealed 12-15 minutes under heating, pressurization using tablet press machine, water flowing is cooled to room temperature；
S5, by step S4, treated that formpiston carries out demoulding processing, and the micro fluidic device with channel can be obtained.
A kind of working method of high motile sperm screening micro fluidic device, specifically includes the following steps:
T1, it after rinsing inside and outside chamber with deionized water, is dried in 37 DEG C of baking ovens；
T2, take sperm nutrient solution filling with sample injector to inner cavity chamber, filling total amount is 400 μ l；
T3, take the semen sample of mixing filling with sample injector to exocoel, filling total amount is 1ml；
T4, the semen sample for taking sperm nutrient solution and the other three to mix with method, it is filling to other three interior exocoels respectively
Room；
T5, with sealing film capping apparatus surface, that is, cover 4 interior exocoels opening, device is then horizontally placed at 37
In DEG C wet box；
T6, semen sample in exocoel is absorbed after 1 hour, and collect the sperm in inner cavity chamber containing high motile sperm and seek
Nutrient solution；
T7, this sperm nutrient solution containing high motile sperm can be directly used for it is in vitro fertilization or therefrom picking sperm carry out ovum
Intracytoplasmic sperm injection in cytoplasm.
Embodiment 2
A kind of high motile sperm screening micro fluidic device, including cylinder, the inner cavity of the cylinder pass through thin layer annular wall point
Inside and outside two chambers are divided into, and inner cavity chamber and exocoel are concentric structure, full sperm nutrient solution, the exocoel are set by inner cavity chamber
Room is equipped with semen sample, and semen sample is made just to cover inner cavity chamber upper surface, and inner cavity chamber radius is 2.5mm, and depth is
20mm, the net radius of exocoel annular are 2mm, depth 20mm, the thin layer annular wall between the interior exocoel with a thickness of
0.5mm is highly 20mm, and sperm nutrient solution is commercialization Earle ' s liquid, and male patient of the semen sample from infertile couples.
A kind of preparation method of high motile sperm screening micro fluidic device, specifically includes the following steps:
S1, Ni-based, monocrystalline silicon or glass mold plungers are prepared by micro fluidic device design drawing；
S2, polymetylmethacrylate sheet material is cut into small pieces by formpiston size, cleaned, drying；
S3, by step S2, treated that polymetylmethacrylate sheet material is loaded to formpiston, compresses；
S4, PMMA is sealed 12-15 minutes under heating, pressurization using tablet press machine, water flowing is cooled to room temperature；
S5, by step S4, treated that formpiston carries out demoulding processing, and the micro fluidic device with channel can be obtained.
A kind of working method of high motile sperm screening micro fluidic device, specifically includes the following steps:
T1, it after rinsing inside and outside chamber with deionized water, is dried in 37 DEG C of baking ovens；
T2, take sperm nutrient solution filling with sample injector to inner cavity chamber, filling total amount is 400 μ l；
T3, take the semen sample of mixing filling with sample injector to exocoel, filling total amount is 1ml；
T4, the semen sample for taking sperm nutrient solution and the other three to mix with method, it is filling to other three interior exocoels respectively
Room；
T5, with sealing film capping apparatus surface, that is, cover 4 interior exocoels opening, device is then horizontally placed at 37
In DEG C wet box；
T6, semen sample in exocoel is absorbed after 1 hour, and collect the sperm in inner cavity chamber containing high motile sperm and seek
Nutrient solution；
T7, this sperm nutrient solution containing high motile sperm can be directly used for it is in vitro fertilization or therefrom picking sperm carry out ovum
Intracytoplasmic sperm injection in cytoplasm.
Embodiment 3
A kind of high motile sperm screening micro fluidic device, including cylinder, the inner cavity of the cylinder pass through thin layer annular wall point
Inside and outside two chambers are divided into, and inner cavity chamber and exocoel are concentric structure, full sperm nutrient solution, the exocoel are set by inner cavity chamber
Room is equipped with semen sample, and semen sample is made just to cover inner cavity chamber upper surface, and inner cavity chamber radius is 2.5mm, and depth is
20mm, the net radius of exocoel annular are 2mm, depth 20mm, the thin layer annular wall between the interior exocoel with a thickness of
0.5mm is highly 20mm, and sperm nutrient solution is BWW liquid, and male patient of the semen sample from infertile couples.
A kind of preparation method of high motile sperm screening micro fluidic device, specifically includes the following steps:
S1, Ni-based, monocrystalline silicon or glass mold plungers are prepared by micro fluidic device design drawing；
S2, polymetylmethacrylate sheet material is cut into small pieces by formpiston size, cleaned, drying；
S3, by step S2, treated that polymetylmethacrylate sheet material is loaded to formpiston, compresses；
S4, PMMA is sealed 12-15 minutes under heating, pressurization using tablet press machine, water flowing is cooled to room temperature；
S5, by step S4, treated that formpiston carries out demoulding processing, and the micro fluidic device with channel can be obtained.
A kind of working method of high motile sperm screening micro fluidic device, specifically includes the following steps:
T1, it after rinsing inside and outside chamber with deionized water, is dried in 37 DEG C of baking ovens；
T2, take sperm nutrient solution filling with sample injector to inner cavity chamber, filling total amount is 400 μ l；
T3, take the semen sample of mixing filling with sample injector to exocoel, filling total amount is 1ml；
T4, the semen sample for taking sperm nutrient solution and the other three to mix with method, it is filling to other three interior exocoels respectively
Room；
T5, with sealing film capping apparatus surface, that is, cover 4 interior exocoels opening, device is then horizontally placed at 37
In DEG C wet box；
T6, semen sample in exocoel is absorbed after 1 hour, and collect the sperm in inner cavity chamber containing high motile sperm and seek
Nutrient solution；
T7, this sperm nutrient solution containing high motile sperm can be directly used for it is in vitro fertilization or therefrom picking sperm carry out ovum
Intracytoplasmic sperm injection in cytoplasm.
Without departing from the principle of the present invention, the shape and size of interior exocoel can improve the present invention, add
Work material and mode also can be different, these improvement are regarded as protection scope of the present invention.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, without necessarily requiring or implying between these entities or operation
There are any actual relationship or orders.Moreover, the terms "include", "comprise" or its any other variant are intended to
Cover non-exclusive inclusion, so that the process, method, article or equipment for including a series of elements not only includes that
A little elements, but also including other elements that are not explicitly listed, or further include for this process, method, article or
The intrinsic element of equipment.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of high motile sperm screens micro fluidic device, including cylinder, it is characterised in that: the inner cavity of the cylinder passes through thin layer
Annular wall is separated into inside and outside two chambers, and inner cavity chamber and exocoel are concentric structure.
2. a kind of high motile sperm according to claim 1 screens micro fluidic device, it is characterised in that: the inner cavity chamber sets
Full sperm nutrient solution, the exocoel is equipped with semen sample, and semen sample is made just to cover inner cavity chamber upper surface.
3. a kind of high motile sperm according to claim 1 screens micro fluidic device, it is characterised in that: the inner cavity chamber half
Diameter is 2.5mm, depth 20mm, and the net radius of exocoel annular is 2mm, depth 20mm, between the interior exocoel
Thin layer annular wall is highly 20mm with a thickness of 0.5mm.
4. a kind of high motile sperm according to claim 2 screens micro fluidic device, it is characterised in that: the sperm nutrition
Liquid is voluntarily to prepare sperm nutrient solution, commercialization one of Earle ' s liquid or BWW liquid or a variety of combinations, and semen sample comes
The male patient of self-sterility Mr. and Mrs.
5. a kind of high motile sperm according to claim 2 screens micro fluidic device, it is characterised in that: the sperm nutrition
Liquid is composed of the following components in voluntarily process for preparation: NaCl 0.721g, MgSO4·7H2O 0.247g, fructose 1g,
Na2HPO4 3.581g、KH2PO40.136g and pure water 1000ml.
6. a kind of high motile sperm according to claim 1-5 screens micro fluidic device, it is characterised in that: it is made
Preparation Method specifically includes the following steps:
S1, Ni-based, monocrystalline silicon or glass mold plungers are prepared by micro fluidic device design drawing；
S2, polymetylmethacrylate sheet material is cut into small pieces by formpiston size, cleaned, drying；
S3, by step S2, treated that polymetylmethacrylate sheet material is loaded to formpiston, compresses；
S4, PMMA is sealed 12-15 minutes under heating, pressurization using tablet press machine, water flowing is cooled to room temperature；
S5, by step S4, treated that formpiston carries out demoulding processing, and the micro fluidic device with channel can be obtained.
7. a kind of high motile sperm according to claim 1-5 screens micro fluidic device, it is characterised in that: its work
Make method specifically includes the following steps:
T1, it after rinsing inside and outside chamber with deionized water, is dried in 37 DEG C of baking ovens；
T2, take sperm nutrient solution filling with sample injector to inner cavity chamber, filling total amount is 400 μ l；
T3, take the semen sample of mixing filling with sample injector to exocoel, filling total amount is 1ml；
T4, the semen sample for taking sperm nutrient solution and the other three to mix with method, it is filling to other three interior exocoels respectively；
T5, with sealing film capping apparatus surface, that is, cover 4 interior exocoels opening, then by device be horizontally placed at 37 DEG C it is wet
In box；
T6, semen sample in exocoel is absorbed after 1 hour, and collect the sperm nutrient solution for containing high motile sperm in inner cavity chamber；
T7, this sperm nutrient solution containing high motile sperm can be directly used for it is in vitro fertilization or therefrom picking sperm carry out ooplasm
Interior intracytoplasmic sperm injection.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910465724.6A CN110172390A (en) | 2019-05-30 | 2019-05-30 | A kind of high motile sperm screening micro fluidic device |
| PCT/CN2019/091172 WO2020237739A1 (en) | 2019-05-30 | 2019-06-13 | Microfluidic device for screening high-motility sperms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910465724.6A CN110172390A (en) | 2019-05-30 | 2019-05-30 | A kind of high motile sperm screening micro fluidic device |
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| CN110172390A true CN110172390A (en) | 2019-08-27 |
Family
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|---|---|---|---|
| CN201910465724.6A Pending CN110172390A (en) | 2019-05-30 | 2019-05-30 | A kind of high motile sperm screening micro fluidic device |
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| CN (1) | CN110172390A (en) |
| WO (1) | WO2020237739A1 (en) |
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| JP2003289850A (en) * | 2002-04-05 | 2003-10-14 | Nipro Corp | Petri dish for selective collection of sperm |
| CN202131314U (en) * | 2011-06-28 | 2012-02-01 | 深圳市博锐德生物科技有限公司 | Culture dish for sorting sperms for in vitro fertilization |
| CN107475078A (en) * | 2017-10-12 | 2017-12-15 | 南京精竞生物科技有限公司 | A kind of sperm screening based on microflow control technique and bionic device in vitro fertilization |
| KR20180025679A (en) * | 2016-09-01 | 2018-03-09 | 을지대학교 산학협력단 | Oocytes and early embryos culture dish |
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| WO2010056755A2 (en) * | 2008-11-11 | 2010-05-20 | Craig H Randall | Microfluidic embryo and gamete culture systems |
| CN201678676U (en) * | 2010-04-30 | 2010-12-22 | 山东省计划生育科学技术研究所 | Nesting type sperm separation and screening tube |
| EP3071704B1 (en) * | 2013-11-20 | 2022-03-30 | Brigham and Women's Hospital, Inc. | System and method for sperm sorting |
| CN105154320A (en) * | 2015-10-12 | 2015-12-16 | 深圳市博锐德生物科技有限公司 | Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device |
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2019
- 2019-05-30 CN CN201910465724.6A patent/CN110172390A/en active Pending
- 2019-06-13 WO PCT/CN2019/091172 patent/WO2020237739A1/en active Application Filing
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| JP2003289850A (en) * | 2002-04-05 | 2003-10-14 | Nipro Corp | Petri dish for selective collection of sperm |
| CN202131314U (en) * | 2011-06-28 | 2012-02-01 | 深圳市博锐德生物科技有限公司 | Culture dish for sorting sperms for in vitro fertilization |
| KR20180025679A (en) * | 2016-09-01 | 2018-03-09 | 을지대학교 산학협력단 | Oocytes and early embryos culture dish |
| CN107475078A (en) * | 2017-10-12 | 2017-12-15 | 南京精竞生物科技有限公司 | A kind of sperm screening based on microflow control technique and bionic device in vitro fertilization |
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| LARISA MARTYNOVA ET AL.: "Fabrication of Plastic Microfluid Channels by Imprinting Methods", 《ANNAL.CHEM.》 * |
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|---|---|
| WO2020237739A1 (en) | 2020-12-03 |
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Application publication date: 20190827 |