CN1101704C - Blood vessel graft preparing technology using human umbilical artery - Google Patents
Blood vessel graft preparing technology using human umbilical artery Download PDFInfo
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- CN1101704C CN1101704C CN98111325A CN98111325A CN1101704C CN 1101704 C CN1101704 C CN 1101704C CN 98111325 A CN98111325 A CN 98111325A CN 98111325 A CN98111325 A CN 98111325A CN 1101704 C CN1101704 C CN 1101704C
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- artery
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Abstract
The present invention relates to a blood vessel graft preparation process for the operation of blood vessel grafting, which comprises the following steps: firstly, under an aseptic condition, the fresh umbilical blood vessel of a human fetus is taken and washed; a fine and straight glass rod is taken, sterilized and inserted into the artery of the human fetus; the peripheral tissues of the artery of the human fetus are peeled off to obtain a blood vessel section; the blood vessel section is suspended in a container, and the container is placed in a cold well; low temperature liquid nitrogen is continuously added into the cold well for quick temperature reduction; the container in the cold well is heated and dried for drying through a high vacuum machine; the freeze-dried blood vessel section is taken out of the container and is packed in a sterilized test tube in vacuum; a semi-finished product is irradiated by radioactive isotopes; after being irradiated, the blood vessel section in the sterilized test tube is the blood vessel graft required by the present invention.
Description
The present invention relates to a kind of blood vessel graft preparation technology who is used for vascular transplant.
Effective Therapeutic Method of common coronary heart disease is that coronary artery is close to the road transplantation, selects fine blood vessel graft most important.At present, generally adopt self great saphenous vein or various tremulous pulse (as internal mammary artery, radial artery or right gastroepiploic artery, inferior epigastric artery etc.).But also there are some shortcomings in these blood vessels, as:
1, adopts great saphenous vein, sometimes vein itself just has pathological changes, as inflammation of vein, varicosis, venous thrombosis, congenital malformation or shank intractable ulcer etc. is arranged, all be difficult for adopting, the difficulty of drawing materials, and vein stops up easily, and 10 years patency rates have only 68% behind the bypass operation of coronary artery, if coronary artery bypass surgery once more, human body self does not just have vein to use again.
2, adopt other various tremulous pulsies,, also do not have other tremulous pulsies to utilize if be used for coronary artery bypass surgery once more.Because above-mentioned two kinds of methods all adopt native blood vessel, thus the difficulty of drawing materials, can't commercialization.
3, adopt terylene (Dacron) or polytetrafluoroethylene (PTFE) artificial blood vessel, but though commercialization, but it is still at the experimental stage at present, need in tube chamber, plant endotheliocyte, laboratory condition requires high, and operating technology is quite complicated, but effect is unsatisfactory, application clinically also has sizable gap, at present also can't application.
Purpose of the present invention is exactly in order to address the above problem, and proposes a kind of transplantings no any rejection in back and survival period length, the blood vessel antigenicity is low and draw materials easy, but commercialization and the effective preparation technology of human umbilical artery that use as blood vessel graft.
Technical solution of the present invention:
A kind of with the preparation technology of human umbilical artery as blood vessel graft, elder generation gets the umbilical blood vessels of Freshman fetus, and washes repeatedly with heparin-saline from the umbilical artery opening part under aseptic condition, it is characterized in that:
(1) gets thin straight Glass rod sterilization back and insert human umbilical artery, its blood vessel is stretched, peel off its surrounding tissue, make vessel segment;
(2) vessel segment is arranged in the container together with Glass rod is unsettled, and this container is placed in the cold-trap, constantly add low temperature liquid nitrogen in cold-trap, fast cooling is to-60～-80 ℃;
(3) container in the cold-trap is made human umbilical artery vessel segment rewarming drying gradually in the container by the fine vacuum machine, obtain the lyophilizing vessel segment of human umbilical artery;
(4) in container, take out lyophilizing vessel segment Vacuum Package in sterilized test tube, obtain the semi-finished product of blood vessel graft of the present invention;
(5) with above-mentioned semi-finished product radiosiotope irradiation, be under the 1.2-1.4 condition at radiation source intensity, exposure time was controlled at 6～8 seconds, and irradiation dose is controlled at 2-11GY, and the invisible spectro vessel segment behind the irradiation is the desired blood vessel graft of the present invention.
The blood vessel graft of the present invention's preparation meets blood vessel (of the same race or xenogenesis) graft three big primary conditions:
1, the antigenicity of blood vessel is low: by lyophilizing and irradiance method, the existing of essentially no cell composition (comprising endotheliocyte),, make after transplanting any rejection does not take place so the antigenicity of blood vessel eases down to bottom line, blood vessel graft can long-term surviving, keeps clear.This is one of key issue of any organ or tissue transplanting.
2, the biophysics of blood vessel is functional:
A, blood vessel elasticity are good: the viscoelasticity property of Freshman umbilical artery, after handling, be transformed into linear stress---strain stress relation, and belong to the performance of favorable elasticity body, in the scope that human blood-pressure changes, can keep normal function; B, vascular compliance are good: after special handling, its compliance is improved under higher pressure; C, blood vessel bridging property are good: behind the irradiation, not only do not damage the collagen fiber of blood vessel, be fit to material framework, and macromolecular material is behind high-energy ray irradiation as blood vessel graft, the crosslink density that intermolecular formation is certain, thereby the mechanical strength and the elastic performance of raising material.
3, the histocompatibility of blood vessel is superior: identical by putting between Canis familiaris L. carotid artery, and the pathological observation of employing microscope and Electronic Speculum, 4 weeks of postoperative, the good no intimal thickening phenomenon of endotheliocyte regeneration in the lumen of vessels.
In addition, of the present invention drawing materials is human umbilical artery, because right and wrong are drawn materials from body, so draw materials conveniently, be convenient to preserve, price is suitable, national conditions had both been met, reach safe and reliable purpose again, and can prepare and become finished commercial prod, can adopt at any time, very convenient, can preserve under the room temperature, but penetration and promotion has very important social benefit and economic benefit.
Below preparation technology of the present invention is described in detail as follows:
1, under aseptic condition, gets the umbilical blood vessels (gathering encapsulation immediately after being preferably in childbirth) of Freshman fetus.
2, from the umbilical artery opening part, syringe needle washes (per 500 ml physiological salines add 100 milligrams of heparin) repeatedly with heparin-saline after inserting.
3, the thin straight Glass rod of the long disinfectant of cut-off footpath 2-3mm, 15cm inserts umbilical artery, blood vessel is stretched, carefully peel off its surrounding tissue but injured blood vessel adventitia not, free to the length that needs blood vessel (general 10-12cm), cut off umbilical artery, form a vessel segment, desirable approximately at every turn 8-10 root vessel segment.If required vessel segment length requirement is longer, can adopt longer thin straight Glass rod.
4, vessel segment (together with Glass rod) setting is fixed in the container,, each vessel segment is plugged in each hole of porous grillage, can make their unsettled perpendicular putting in container as being provided with the porous grillage at the container inner bottom part.Container is put in the cold-trap again, and constantly adds low temperature liquid nitrogen in cold-trap, fast cooling is to-70 ℃, and temperature is by copper — — Opinions-Pictures copper thermocouple monitoring.
5, connect the fine vacuum machine, utilize the combination air-extractor of fine vacuum to reach the progressively exsiccant purpose of rewarming.Use fine vacuum facility body step as follows:
(1) starting of unit at first connected the mechanical pump power supply, and preceding step valve and diffusion pump valve are all opened, and carries out total system and finds time in advance.
(2) reach 5 * 10 when system
-2During torr, can connect the diffusion pump power supply, open water valve simultaneously pump is cooled off, discharge be not less than 100 liters/time.
(3) the dry end of rewarming will be shut down, and should cut off the diffusion pump power supply earlier, wait a few minutes after, again with the diffusion pump valve closing, close the prime valve simultaneously, the cutting machine pumping source is put into atmosphere with mechanical pump at last, cooling water can be closed after waiting tens minutes again.
6, reach 1 * 10 when container vacuum
-3Kept behind the mmHg 2 hours, and took out freeze dried vessel segment and carry out the teat glass encapsulation.
7, the human umbilical artery vessel segment with the test tube encapsulation send atomic energy research unit to use
60Co-gamma-radiation, radiation source intensity are 1.37, and the time is 6.5 seconds, dosage 6～8GY.Be the desired blood vessel graft finished product of the present invention behind the irradiation, it can be preserved at normal temperatures, can commercialization.The purpose of irradiation, the one, sterilization, the 2nd, with the endotheliocyte destruction of umbilical artery, reach and reduce the antigenic purpose of umbilical artery.Confirm that through zoopery and pathologic finding the umbilical artery endotheliocyte is regenerated and finished in week in 2-4, inner membrance does not have the propagation phenomenon.
When the blood vessel graft of the present invention's preparation is used in operation, its operation must be carried out under aseptic condition, the test tube of packaged sterile blood vessel graft at first, take out vessel segment then, be immersed in 37-42 ℃ of normal saline interior 15-30 minute, and the thinner straight Glass rod in the vessel segment extracted out, so just reach the purpose of rehydration, it is organized and reverts to similar fresh human umbilical artery immediately, the blood vessel transplantation during promptly can be used for performing the operation this moment.
The present invention makes blood vessel graft by freezing, drying and irradiation three big steps.Owing to through radiation treatment, make that the cell composition of human umbilical artery vessel segment all disappears (not only endotheliocyte), antigenicity reduces, remaining is the fiber framework, Main Ingredients and Appearance is collagen fiber, elastic fiber and smooth muscle fiber, and they all belong to polymer substance, uses
60The gamma-ray irradiation of Co-, certain " cross-linking reaction " of formation between its fiber molecule will be made, stop the inflammatory cell intrusion, thereby avoid intimal proliferation, blood vessel graft " restenosis " can not appear, thoroughly solved " obstruction " difficult problem of blood vessel graft, for obtaining of blood vessel graft provides a brand-new approach.
Claims (1)
1, a kind of with the preparation technology of human umbilical artery as blood vessel graft, elder generation gets the umbilical blood vessels of Freshman fetus, and washes repeatedly with heparin-saline from the umbilical artery opening part under aseptic condition, it is characterized in that:
(1) gets thin straight Glass rod sterilization back and insert human umbilical artery, its blood vessel is stretched, peel off its surrounding tissue, make vessel segment;
(2) vessel segment is arranged in the container together with Glass rod is unsettled, and this container is placed in the cold-trap, constantly add low temperature liquid nitrogen in cold-trap, fast cooling is to-60～-80 ℃;
(3) container in the cold-trap is made human umbilical artery vessel segment rewarming drying gradually in the container by the fine vacuum machine, and kept 2 hours, obtain the lyophilizing vessel segment of human umbilical artery;
(4) take out the lyophilizing vessel segment in the container and reach 1 * 10 in vacuum
-3Be packaged in the sterilized test tube under the mmHg condition, obtain the semi-finished product of blood vessel graft of the present invention;
(5) with above-mentioned semi-finished product radiosiotope irradiation, be under 1.2～1.4 conditions at radiation source intensity, exposure time was controlled at 6～8 seconds, and irradiation dose is controlled at 2～11GY, and the invisible spectro vessel segment behind the irradiation is the desired blood vessel graft of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98111325A CN1101704C (en) | 1998-05-28 | 1998-05-28 | Blood vessel graft preparing technology using human umbilical artery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98111325A CN1101704C (en) | 1998-05-28 | 1998-05-28 | Blood vessel graft preparing technology using human umbilical artery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1199644A CN1199644A (en) | 1998-11-25 |
| CN1101704C true CN1101704C (en) | 2003-02-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98111325A Expired - Fee Related CN1101704C (en) | 1998-05-28 | 1998-05-28 | Blood vessel graft preparing technology using human umbilical artery |
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| Country | Link |
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| CN (1) | CN1101704C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114504682A (en) * | 2021-11-16 | 2022-05-17 | 李平 | Application of umbilical artery in preparation of coronary bypass graft blood vessel material and method for preparing coronary bypass graft blood vessel material |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079912A (en) * | 1991-11-26 | 1993-12-29 | 研究发展基金会 | The fetal membrane pipe that is used for neural and blood vessel transplantation |
-
1998
- 1998-05-28 CN CN98111325A patent/CN1101704C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079912A (en) * | 1991-11-26 | 1993-12-29 | 研究发展基金会 | The fetal membrane pipe that is used for neural and blood vessel transplantation |
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|---|---|
| CN1199644A (en) | 1998-11-25 |
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