CN110170305A - A kind of separating technology of polylysine - Google Patents
A kind of separating technology of polylysine Download PDFInfo
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- CN110170305A CN110170305A CN201910366139.0A CN201910366139A CN110170305A CN 110170305 A CN110170305 A CN 110170305A CN 201910366139 A CN201910366139 A CN 201910366139A CN 110170305 A CN110170305 A CN 110170305A
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
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Abstract
The invention discloses a kind of separation methods of epsilon-polylysine, the separation method are as follows: adsorbent is added into epsilon-polylysine fermentation liquid, the adsorbent is that nano-scale magnetic ferroso-ferric oxide is either modified the improved ferroferric oxide obtained to nano-scale magnetic ferroso-ferric oxide by lauryl sodium sulfate, dodecyl sodium sulfate or sodium citrate, sufficiently adsorbent is separated with strong magnet after absorption, isolated adsorbent is eluted with ammonium hydroxide after washing, obtains epsilon-polylysine eluent.Magnetic Nano material (i.e. nano-scale magnetic ferroso-ferric oxide) provided by the invention has unique superparamagnetism, the special selection adsorption effect modified and improve it for the epsilon-polylysine in epsilon-polylysine fermentation liquid especially is carried out to magnetic Nano material, shorten separation cycle, separating effect is improved, and separating technology is easy to operate, cost is relatively low.
Description
Technical field
The present invention relates to a kind of separating technologies of novel polylysine.
Technical background
Epsilon-polylysine (ε-poly-L-lysine, ε-PL) is the L-lysine homopolymer by Microbe synthesis, by ɑ-carboxylic
Base and epsilon-amino are formed by connecting by peptide bond, and the degree of polymerization is generally 25-35, and molecular weight is 2500-4500 Da.ε-PL most earlier than
1977 by the island Janpanese sake well Ping Yihe clear two in largely screening D.P. positive material (alkaloid) from streptomyces albus
It is found in (Streptomyces albulus) fermentation liquid.Just great et al. the discovery S.noursei of Japan Teng Jing in 1989 also can
Generate ε-PL.Hereafter the bacterial strain that can much generate ε-PL, such as filamentous fungi, kitasatosporia, bacillus are had found, but more
Concentrate on Streptomycetaceae.High-cation ingredient specific to ε-PL has security performance height and biodegradable property.ε-
PL is because it has many advantages, such as that antimicrobial spectrum is wide, stability is strong, good water solubility, safe and non-toxic, successively in states such as Japan, South Korea, the U.S.
Family and area are widely used in food antiseptic field.In addition, ε-PL is also used for answering for the fields such as pharmaceutical carrier, genetic chip
With research.
Therefore, ε-PL be considered as it is a kind of with essential industry value infant industry biological technology products, in recent years by
To the concern of more and more food and technical field of biological material.The preparation of ε-PL mainly includes two parts: microbial fermentation, extraction
With purification.Currently, industrially to be mainly streptomyces albus obtain superior strain by mutagenesis to the strain of production epsilon-polylysine, gather
The separation of fermenting lysine product is cumbersome, fermentation broth contents are complicated, ion exchange column is relied primarily in separation process separate it is pure
Change.Ion-exchange chromatography is the side separated using the difference of binding force between charged particles various in solution and exchanger
Method, epsilon-polylysine is positively charged, preferably cation exchange resin is used to be separated.Conventional separating technology are as follows: fermentation ends
Afterwards, ion exchange resin is added, stirring completes Static Adsorption, after absorption, resin is filtered out, and will be set with a large amount of water
Rouge rinses about 20 hours, then parse using hydrochloric acid attached, and after nanofiltration is concentrated, is spray-dried, obtains product.It should
Separating technology has the following problems:, when carrying out isolate and purify target product friendship heavy using cation exchange resin lock out operation
Throw-over degree is slow, and separation process is long, needs to expend great lot of water resources, is easy to cause water-quality deterioration etc..
Therefore it provides a simple process, separation cycle is short, environmental pollution is small, the poly- bad ammonia of effective separate epsilon-
The method of acid is the target that the technical field needs to explore.
Summary of the invention
It is an object of the invention to provide a kind of simple process, separation cycle is short, environmental pollution is small, effective fortune
With the method for magnetic Nano material separation epsilon-polylysine.
For achieving the above object, the present invention adopts the following technical scheme:
A kind of separation method of epsilon-polylysine, the separation method are as follows: absorption is added into epsilon-polylysine fermentation liquid
Agent, the adsorbent are that nano-scale magnetic ferroso-ferric oxide either passes through lauryl sodium sulfate, dodecyl sodium sulfate
Or sodium citrate is modified to nano-scale magnetic ferroso-ferric oxide and the improved ferroferric oxide that obtains, sufficiently with strong after absorption
Magnet separates adsorbent, and isolated adsorbent is eluted with ammonium hydroxide after washing, obtains epsilon-polylysine eluent.
Epsilon-polylysine fermentation liquid of the present invention is fermented using production epsilon-polylysine bacterium.It finally obtains
Epsilon-polylysine eluent can be obtained epsilon-polylysine finished product through regular refiner, i.e., after nanofiltration is concentrated, done by spraying
It is dry, it can be obtained epsilon-polylysine product.
Preferably, the concentration of the ammonium hydroxide is 0.1M-0.4M, the rate of recovery is increased with the increase of ammonia concn,
But after ammonia concn is more than 0.4M, then there is the reduction of the rate of recovery.More preferable ammonia concn is 0.3M-0.4M.
Preferably, the adsorbent is by lauryl sodium sulfate, dodecyl sodium sulfate or sodium citrate to receiving
Meter level magnetic ferroferric oxide is modified and the improved ferroferric oxide that obtains, most preferably by sodium citrate to nanoscale
Magnetic ferroferric oxide is modified and the improved ferroferric oxide that obtains.
In the present invention, the adsorbent is by lauryl sodium sulfate, dodecyl sodium sulfate or sodium citrate pair
Nano-scale magnetic ferroso-ferric oxide is modified and obtains, and above-mentioned method of modifying is the prior art, and those skilled in the art can
Implement to be modified in method according to the literature.
When modified material is lauryl sodium sulfate or dodecyl sodium sulfate, the present invention specifically recommends the adsorbent
It is prepared as follows: nano-scale magnetic ferroso-ferric oxide is mixed with deionized water, extremely with sodium hydroxide solution tune pH
11.5, it heats up under stirring, when temperature rises to 80 DEG C, lauryl sodium sulfate or dodecyl sodium sulfate is added, makes
The mass ratio of nano-scale magnetic ferroso-ferric oxide and lauryl sodium sulfate or dodecyl sodium sulfate is 1:1, is continuously heating to
85 DEG C insulation reaction 1.5 hours, after reaction, be cooled to room temperature to reacting liquid temperature, reaction solution poured into beaker, with strength
Magnet carries out Magneto separate, and it is 7 that obtained solid substance, which is washed with deionized to the pH of cleaning solution, to obtain adsorbent.Gained
Adsorbent is configured to aqueous solution, and sealing saves backup.
When modified material is sodium citrate, the present invention specifically recommends the adsorbent to prepare in accordance with the following steps:
(1) green vitriol, anhydrous ferric chloride and anhydrous citric acid sodium are dissolved in deionized water and obtain mixing water
Solution (the preferably described green vitriol and the feed ratio of deionized water be 0.01mol:50mL), wherein seven hydrated sulfuric acids are sub-
The molar ratio of iron, anhydrous ferric chloride and anhydrous citric acid sodium is 1:2:1；
(2) in nitrogen atmosphere, the temperature of mixed aqueous solution is risen to 50 DEG C under stiring, with the speed of two to three drop per second
Sodium hydrate aqueous solution (preferably its concentration is 0.1M) is added dropwise in degree, until reaction solution blackening, is continuously heating to 55 DEG C of insulation reactions 1
Hour, after reaction, adjustment temperature is 60 DEG C, closes stirring, is cured 30 minutes；
(3) it after curing, is cooled to room temperature to reaction solution, is separated with strong magnet, obtained solid substance adds deionized water
Washing is equal to 7 to cleaning solution pH to get adsorbent is arrived.Gained adsorbent saves backup in deionized water.
Compared with prior art, present invention has an advantage that
Magnetic Nano material (i.e. nano-scale magnetic ferroso-ferric oxide) provided by the invention has unique superparamagnetism, right
Magnetic Nano material carries out the selection absorption that special modification improves it for the epsilon-polylysine in epsilon-polylysine fermentation liquid
Effect shortens separation cycle, improves separating effect, and separating technology is easy to operate, cost is relatively low.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This.
Epsilon-polylysine fermentation liquid in the present invention is prepared referring to following documents: [Chen Xiong, Zhang Ying, Yuan Jinfeng, Wang Shiyu,
The poly- ε of Wang Jinhua-lysine fed-batch fermentation Primary Study industrial microorganism, the 4th phase of volume 37, in August, 2007].
Embodiment 1: the preparation method of magnetic Nano material
The present invention selects relatively simple wet-chemical coprecipitation to receive as preparation premised on laboratory condition and safety
The main method of meter level magnetic ferroferric oxide.Specific implementation step is as follows:
(1) according to chemical equation Fe2++2Fe3++8OH-=Fe3O4+4H2O accurately weighs 2.8g green vitriol,
3.24g anhydrous ferric trichloride is dissolved in respectively in 50mL deionized water, is transferred in 500mL three-neck flask and is mixed.By three-neck flask
It is fixed in oil bath pan, is put into magnetic stir bar.
(2) 4g sodium hydroxide is accurately weighed, is dissolved in 100mL deionized water, is transferred to after solution temperature is down to room temperature
In constant pressure dropper, and install to three-neck flask.
(3) setting reaction temperature is 55 DEG C, opens magnetic agitation.When temperature rises to 50 DEG C or so, constant pressure drop is opened
Sodium hydroxide solution is added dropwise with the speed of two to three drop per second, until mixed liquor blackening, keeps the item of 55 DEG C and magnetic agitation in pipe
Part reacts 90min.
(4) after reaction, erect three-neck flask, be cooled to room temperature to it, reaction solution is poured into beaker, with strong magnet into
Row Magneto separate removes supernatant, and 150mL deionized water is added, and removes magnet and agitator treating, then separated with magnet, goes
Clear liquid.Repeatedly, until the pH of last time deionized water is 7.
(5) it is configured to the solution of 0.01mol/100mL, is stored in conical flask, preservative film sealing.
(6) above step is repeated, prepares identical 4 parts altogether.
Embodiment 2-4: the surface modification and modification of magnetic Nano material
Lauryl sodium sulfate, dodecyl sodium sulfate and cetyl trimethylammonium bromide is selected to be used as to magnetic Nano
The substance of material surface modification.The method of modifying of three kinds of materials is identical as condition.
The preparation method is specifically according to being implemented as follows:
It is prepared into above-described embodiment 1 in obtained same 3 parts of magnetic Nano materials and is separately added into 100mL deionization
Water is put into magnetic stir bar, and install with being transferred in clean three-neck flask after the sodium hydroxide solution tune pH to 11.5 of 1M
In oil bath pan, magnetic agitation is opened.It is 85 DEG C that reaction temperature, which is arranged, when temperature rises to 80 DEG C, according to magnetic Nano material
The amount of material and surfactant qualities ratio 1:1, is separately added into above-mentioned three kinds of surfactants.85 DEG C of reaction environments are maintained, are continued
Reaction 1.5 hours.After reaction, when reacting liquid temperature is down to room temperature, reaction solution is poured into beaker, is carried out with strong magnets
Magneto separate removes supernatant, and 150mL deionized water is added, and removes magnet and agitator treating, then separated with magnet, removes supernatant
Liquid.Repeatedly, until the pH of last time deionized water is 7.It is finally configured to the solution of 0.01mol/100mL, in 250mL
Taper bottle closure saves backup.
Table 1
| Embodiment | Modified material |
| 2 | Lauryl sodium sulfate |
| 3 | Dodecyl sodium sulfate |
| 4 | Cetyl trimethylammonium bromide |
Embodiment 5
It is different from the method for modifying of above-mentioned surfactant to the modification of nanoscale ferroso-ferric oxide with sodium citrate.Specifically
Implementation method is as follows:
(1) 2.8g green vitriol, 3.24g anhydrous ferric chloride and 2.94g anhydrous citric acid sodium are weighed, is dissolved in respectively
It in 50mL deionized water, is transferred in 500mL three-neck flask, and be mounted in oil bath pan, magnetic stir bar is added.
(2) 4g sodium hydroxide is accurately weighed, is dissolved in 100mL deionized water, is transferred to after solution temperature is down to room temperature
In constant pressure dropper, and install to three-neck flask.
(3) it goes the dropper of rubber head to be tightly connected in a balloon and one with sealed membrane, pours into a certain amount of nitrogen, pass through
Rubber stopper is fixed on the middle of three-neck flask, unclamps balloon, is drained the air in three-neck flask using nitrogen.
(4) 55 DEG C of oil baths are set, magnetic agitation is opened, when temperature rises to 50 DEG C or so, with two to three drop per second
Sodium hydroxide solution is added dropwise in speed, until reaction solution blackening, maintains 55 DEG C to react 1 hour.After reaction, adjustment temperature is 60
DEG C, stirring is closed, is cured 30 minutes.
(5) it after curing, is cooled to room temperature to solution, pours into beaker, after being separated with strong magnet plus deionized water is washed
After being equal to 7 to pH, supernatant is removed, 100mL deionized water is poured into and saves backup.
Embodiment 6-9: optimal adsorption time test
It is attempted according to multiple, finally determination selects concentration most preferably to be inhaled for 1% epsilon-polylysine fermentation liquid to the present invention
The test of attached time.Specific embodiment is as follows:
6 clean 250mL conical flasks are taken, number consecutively 0-5, are separately added into 50mL epsilon-polylysine solution.1-
It is separately added into that 10g is above-mentioned to be prepared into adsorbent material in No. 5 conical flasks.In order to exclude experiment interference, select No. 0 for experiment contrast,
10mL deionized water is added wherein.It is specific corresponding as shown in table 2 below:
Table 2
6 conical flasks are sealed with preservative film, are put into 25 DEG C of shaking tables, select 0.5h, 2h, 4h, 6h, 12h, this 6 for 24 hours
Time point is sampled.The separation of Shi Xianyong strong magnet is sampled, then is managed with the liquid-transfering gun Aspirate supernatant of 1000 μ L in 1.5mLEP
It is interior, it is put into centrifuge, is centrifuged 2min under the conditions of 12000r/min, then with 0.22 μm of water film filtering.Each sample is all put on
Number and sample time carry out liquid phase analysis, and the results are shown in Table 3:
Table 3: several modified material adsorption ratiosa(%):
aThe original of epsilon-polylysine in quality/fermentation liquid of adsorption ratio (%)=adsorbent material absorption epsilon-polylysine
Prothyl amount * 100%
By above-mentioned chart it is found that not modified magnetic Nano material adsorption effect is most when adsorption time is 0.5h
It is good, but only 21.97%, and adsorption effect is with the growth presentation downward trend of adsorption time.Cetyl trimethyl bromine
Change the modified magnetic Nano material adsorption time of ammonium between for 24 hours whole adsorption effect it is bad, the adsorption ratio of any time is all low
In 10%.Adsorption effect is relatively when adsorption time is 0.5h and 4h for the magnetic Nano material being modified by lauryl sodium sulfate
It is good, there is decline in 2h, and adsorption effect gradually decreases after 4h.Pass through the modified magnetic Nano material of dodecyl sodium sulfate
Material adsorption efficiency is integrally in decreasing trend with the extension of time, and the adsorption effect best time is 0.5h, and adsorption ratio is
26.6%.
Finally obtaining the magnetic Nano material being modified by sodium citrate has 75% or more in entire adsorption experiment
Adsorption ratio, adsorption effect is best when 2h, adsorption ratio 96.62%.
Embodiment 10: maximal absorptive capacity test
According to embodiment 6-9's as a result, select the magnetic Nano material being modified by sodium citrate as measurement maximum adsorption
The adsorbent of amount.
In maximal absorptive capacity experiment, directlys adopt fermentation liquid and is tested, specific implementation step is as follows:
5 clean 250mL conical flasks are taken, the fermentation liquid that 50mL is added is respectively corresponded, wherein polylysine content is
20g/L, then it is separately added into each bottle 2,4,6,8, the adsorbent of 5 method of 10g embodiment preparation, it is sealed, is put into preservative film
25 DEG C of shaking tables, according to the optimal adsorption time as a result, selecting absorption 2h is experiment condition.After absorption, separated with strong magnet
Afterwards, it takes upper liquid in the EP pipe of 1.5mL with 1000 μ L liquid-transfering guns, and is centrifuged 2min through 12000r/min centrifuge, then use
0.22 μm of water film filtering, last liquid phase analysis.
According to the peak area and standard curve of polylysine in the fermentation liquid after absorption, experiment knot as shown in table 4 is obtained
Fruit:
Table 4: the adsorbance of each group after absorption
According to upper table, when adsorbent dosage reaches 6 grams, polylysine therein can largely be adsorbed, adsorption rate reaches
To 95%.
The present invention obtains the magnetic Nano material being modified by sodium citrate when the optimal adsorption time is 2h, maximum adsorption
Amount is 0.16g/g.
Embodiment 11: desorption adhesion test
When the present invention is considered with ethanol elution, since absorbent particles are small, pillar Severe blockage, and ethanol elution cannot
It is put into shaking table, so being eluted with ammonium hydroxide.
According to maximal absorptive capacity and optimal adsorption time, use 50mL polylysine fermentation liquid (polylysine content for
It 20g/L) is tested, the adsorbent material of the citric acid-modified of 5 method of 6g embodiment preparation, adsorption time 2h, absorption is added
After, it is separated with strong magnet, is then added 10mL water washing 2 times.It is eluted with the ammonium hydroxide 50mL of 0.5M, carries out liquid phase point
Analysis.After measured, product recovery rate reaches 81.1% after elution.
Embodiment 12: the determination of optimal eluant strength
According to the optimal adsorption time as a result, a kind of adsorbent for selecting adsorption effect best, it is dense to carry out optimal eluant, eluent
The determination of degree.
0.1M, 0.2M, 0.3M are configured, the ammonium hydroxide of the different gradients of this five kinds of 0.4M, 0.5M is eluted.By embodiment 11
Method carries out parsing adhesion test, then carries out liquid phase analysis.
Table 5: the polylysine rate of recovery after the ammonium hydroxide elution of various concentration
According to table, in the concentration range of 0.1M-0.4M, the rate of recovery is increased with the increase of ammonia concn, works as ammonia
After water concentration is more than 0.4M, then there is the reduction of the rate of recovery in (by taking 0.5M concentration as an example).
It is best that result of implementation shows that the magnetic Nano material being modified by sodium citrate has polylysine in fermentation liquid
Adsorption capacity, therefore application of the present invention research magnetic Nano material in bio-separation isolates and purifies tool to polylysine is simplified
It is significant.
It is above-mentioned referring to embodiment be to special material modify magnetic Nano material separation epsilon-polylysine method
The detailed description carried out with absorption property, is illustrative without being restrictive, can be included according to limited range several
A embodiment, therefore the change and modification in the case where not departing from present general inventive concept, should belong within protection scope of the present invention.
Claims (8)
1. a kind of separation method of epsilon-polylysine, it is characterised in that: the separation method are as follows: into epsilon-polylysine fermentation liquid
Adsorbent is added, the adsorbent is that nano-scale magnetic ferroso-ferric oxide either passes through lauryl sodium sulfate, dodecane
Base sodium sulfonate or sodium citrate are modified to nano-scale magnetic ferroso-ferric oxide and the improved ferroferric oxide that obtains, sufficiently inhales
Attached to be separated adsorbent with strong magnet, isolated adsorbent is eluted with ammonium hydroxide after washing, obtains the poly- bad ammonia of ε-
Sour eluent.
2. separation method as described in claim 1, it is characterised in that: the concentration of the ammonium hydroxide is 0.1M-0.4M.
3. separation method as described in claim 1, it is characterised in that: the ammonia concn is 0.3M-0.4M.
4. the separation method as described in one of claim 1-3, it is characterised in that: the adsorbent is to pass through dodecyl sulphate
The modification four that sodium, dodecyl sodium sulfate or sodium citrate are modified nano-scale magnetic ferroso-ferric oxide and obtain aoxidizes three
Iron.
5. separation method as claimed in claim 4, it is characterised in that: the adsorbent is by sodium citrate to nanoscale
Magnetic ferroferric oxide is modified and the improved ferroferric oxide that obtains.
6. the separation method as described in one of claim 1-3, it is characterised in that: when modified material be lauryl sodium sulfate or
When dodecyl sodium sulfate, the adsorbent is prepared as follows: by nano-scale magnetic ferroso-ferric oxide and deionized water
Mixing, with sodium hydroxide solution tune pH to 11.5, heats up under stirring, and when temperature rises to 80 DEG C, dodecyl is added
Sodium sulphate or dodecyl sodium sulfate make nano-scale magnetic ferroso-ferric oxide and lauryl sodium sulfate or dodecyl sodium sulfate
Mass ratio be 1:1, be continuously heating to 85 DEG C of insulation reactions 1.5 hours, after reaction, be down to room temperature to reacting liquid temperature
Afterwards, reaction solution is poured into beaker, carries out Magneto separate with strong magnets, obtained solid substance is washed with deionized to cleaning solution
PH is 7, to obtain adsorbent.
7. the separation method as described in one of claim 1-3, it is characterised in that: described when modified material is sodium citrate
Adsorbent is prepared in accordance with the following steps:
(1) green vitriol, anhydrous ferric chloride and anhydrous citric acid sodium are dissolved in deionized water and obtain mixed aqueous solution,
Wherein the molar ratio of green vitriol, anhydrous ferric chloride and anhydrous citric acid sodium is 1:2:1；
(2) in nitrogen atmosphere, the temperature of mixed aqueous solution is risen to 50 DEG C under stiring, with the speed drop of two to three drop per second
Adding sodium hydroxide aqueous solution, until reaction solution blackening, is continuously heating to 55 DEG C of insulation reactions 1 hour, after reaction, adjustment temperature
Degree is 60 DEG C, closes stirring, is cured 30 minutes；
(3) it after curing, is cooled to room temperature to reaction solution, is separated with strong magnet, obtained solid substance adds deionized water to wash
It is equal to 7 to cleaning solution pH to get adsorbent is arrived.
8. separation method as claimed in claim 7, it is characterised in that: in step (1), the green vitriol and go from
The feed ratio of sub- water is 0.01mol:50mL；In step (2), the concentration of sodium hydrate aqueous solution is 0.1M.
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2019
- 2019-04-30 CN CN201910366139.0A patent/CN110170305B/en active Active
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| CN101934080A (en) * | 2010-09-08 | 2011-01-05 | 西北大学 | Method for improving material blood compatibility by using electrostatic self-assembled coating |
| CN102350309A (en) * | 2011-06-30 | 2012-02-15 | 浙江工业大学 | Endotoxin adsorption medium based on magnetic chitosan microballoon and its application method |
| CN105713194A (en) * | 2016-03-30 | 2016-06-29 | 浙江新银象生物工程有限公司 | Method for purifying epsilon-PL (epsilon-polylysine) |
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| NANI WIBOWO ET AL.: "Epsilon-Polylysine Fermentation and its Recovery Using Carboxymethyl Cellulose (CMC)-Conjugated Magnetite", 《SEPARATION SCIENCE AND TECHNOLOGY》 * |
| 宋庆峰: "纳米四氧化三铁的制备、修饰及磁场的影响", 《中国优秀硕士学位论文全文数据库》 * |
| 荆洁颖: "5.4 一步反向沉淀法制备水分散纳米Fe3O4颗粒", 《高分散纳米催化剂制备及光催化应用》 * |
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