CN110156902A - Tuber of multiflower knotweed polysaccharide and preparation method thereof - Google Patents
Tuber of multiflower knotweed polysaccharide and preparation method thereof Download PDFInfo
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- CN110156902A CN110156902A CN201711436457.7A CN201711436457A CN110156902A CN 110156902 A CN110156902 A CN 110156902A CN 201711436457 A CN201711436457 A CN 201711436457A CN 110156902 A CN110156902 A CN 110156902A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses tuber of multiflower knotweed polysaccharide and preparation method thereof, the polysaccharide is made of galacturonic acid, galactolipin, arabinose, rhamnose, glucuronic acid and mannose, its main component is galacturonic acid, accounts for 45.6%, tuber of multiflower knotweed polysaccharide molecular weight range is 3500-5500Da.The preparation method of tuber of multiflower knotweed polysaccharide of the present invention, including pretreatment of raw material, the preparation of tuber of multiflower knotweed Thick many candies extracting solution, removing protein, the preparation of tuber of multiflower knotweed holosaccharide.It has the beneficial effect that tuber of multiflower knotweed polysaccharide of the present invention has obvious antioxidant activity, Amti-sanility health-care food auxiliary material or function factor can be developed into, be applied to food or field of health care products；Preparation method of the present invention is simple, and the extraction efficiency and yield of polysaccharide are higher, can guarantee polysaccharide anti-oxidative activity.
Description
Technical field
The present invention relates to polysaccharide technical fields, more particularly, to tuber of multiflower knotweed polysaccharide and preparation method thereof.
Background technique
Polysaccharide is one of the most abundant substance of nature content, and nearly three during the last ten years, with biological and chemical science it is fast
Speed development, the various functions of polysaccharide are disclosed.It is found that effect of the carbohydrate in organism functions not only as energy matter
Or structural material, the prior various activities for taking part in cell in biological phenomena.Therefore the research of sugar starts gradually actively to rise
Come.Natural polysaccharide be mainly from nature plant or agricultural and sideline product in extract and be isolated to and there is very Johnson & Johnson's object
There is abundant wild and family to plant plant resources in active China, and Chinese medicine is represented as the plant with medical value, therefore it is lived
Property polysaccharide research attract attention, their physiological activity, chemical structure W and structure-activity relationship become the forward position battle array of polysaccharide researches
Ground, and make great progress.
The fleece-flower root is also known as the tuber of multiflower knotweed, the red tuber of multiflower knotweed, Goblin etc., belongs to polygonum multiflorum thunbPolygonum multiflorumThunb.Dry rhizome.Wild product are distributed in the province such as southwestern, Central-South, East China and Shaanxi, Gansu.It is raw
The fleece-flower root has the function of removing toxic substances, the carbuncle that disappears, relax bowel and defecation, itches for rubeola convulsion, dry constipation of intestines, hyperlipidemia；Prepared fleece flower root has
Nourishing liver and kidney, beneficial to spirit and marrow, black beard and hair, the function of strengthening the bones and muscles, for blood deficiency chlorosis, poliosis, soreness and weakness of waist and knees, extremity numbness,
Metrorrhagia and metrostaxis leukorrhagia, chronic malaria body void, hyperlipidemia.The tuber of multiflower knotweed contains a variety of chemical components such as sterioside class, phospholipid, polysaccharide, both pharmaceutically acceptable,
It is edible, there are extensive pharmacological activity and nutrition health-care functions, it is expensive wide before exploitation.In recent years to the biology of Radix Cynanchi Auriculati polysaccharide
Activity rating is studies have shown that there is Radix Cynanchi Auriculati polysaccharide the activity of immunological regulation, anti-oxidant, anti-aging and anti-senile dementia disease etc. to make
With, but its study subject is mostly water extract, Thick many candies etc..
Summary of the invention
One of the objects of the present invention is to provide a kind of tuber of multiflower knotweed polysaccharide that antioxidant activity is high.
The second object of the present invention is to provide that a kind of preparation method is simple, and the extraction efficiency and yield of polysaccharide are higher,
It can guarantee the preparation method of the active tuber of multiflower knotweed polysaccharide of polysaccharide anti-oxidative.
The present invention in view of the above technology in the problem of mentioning, the technical solution taken are as follows: tuber of multiflower knotweed polysaccharide, the polysaccharide is by gala
Uronic acid, galactolipin, arabinose, rhamnose, glucuronic acid and mannose composition, main component are galacturonic acid,
45.6% is accounted for, tuber of multiflower knotweed polysaccharide molecular weight range is 100-300kDa, and above-mentioned tuber of multiflower knotweed polysaccharide has obvious antioxidant activity, can
To be developed into Amti-sanility health-care food auxiliary material or function factor, it is applied to food or field of health care products.
The preparation method of tuber of multiflower knotweed polysaccharide, including pretreatment of raw material, the preparation of tuber of multiflower knotweed Thick many candies extracting solution, removing protein, the tuber of multiflower knotweed are pure
Polysaccharide preparation, specific preparation step are as follows:
Pretreatment of raw material: will with petroleum ether by solid-liquid ratio be 1:3-5 in radix polygoni multiflori powder, temperature is to take off for 45-55 DEG C of extraction 2-3 times
Rouge processing, is centrifuged 20-25rnin at 3000-5000rpm after extraction, deposit is dried at 50-60 DEG C to get de-
Rouge radix polygoni multiflori powder；
The preparation of tuber of multiflower knotweed Thick many candies extracting solution: being 1:17-22 into degreasing radix polygoni multiflori powder plus water by solid-liquid ratio, temperature be 70-80 DEG C,
Power is ultrasonic extraction 80-100min under 100-150W, then 20-25rnin is centrifuged at 3000-5000rpm, by supernatant
Be concentrated into the 1/4 to 1/2 of original volume, concentrate be tuber of multiflower knotweed Thick many candies extracting solution, utilize ultrasonic wave effect to generate cavitation effect
It answers, forms high pressure in histocyte, the release of high pressure forms powerful shock wave in the solution, can effectively reduce, disappear
Resistance between water removal and the tuber of multiflower knotweed, to increase mass-transfer efficiency, while the physical shear that cell tissue is generated by shock wave
Power and deformation fracture, release intracellular organic matter, have greatly accelerated extraction process；
Removing protein: tuber of multiflower knotweed polysaccharide extraction liquid is added in ionic liquid-inorganic salts double-aqueous phase system, vibrates to obtain mixed system, so
Split-phase 10-15h is stood at 20-30 DEG C afterwards, will mutually separate, and lower phase will be fitted into 350-500kDa bag filter, in distillation up and down
20-30h is dialysed in water so that except desalting and other small molecular weight impurities, resulting dialyzate to be concentrated under reduced pressure into the 0.15- of original volume
0.25 times, spare, ionic liquid-inorganic salts double-aqueous phase system viscosity is smaller, which can will be in tuber of multiflower knotweed polysaccharide extraction liquid
The small molecular weight impurities such as protein, minerals are extracted into phase on double-aqueous phase system, and the distribution of tuber of multiflower knotweed polysaccharide recycles double water in lower phase
Lower phase in phase system, adds alcohol precipitating that the tuber of multiflower knotweed polysaccharide of purifying can be obtained, compared with traditional handicraft, mild condition shortens work
Skill process, reduces energy consumption, reduces consumption of organic solvent, while the available effective recycling and reusing of ionic liquid, drop
Low material consumption is more suitable for large-scale production and application；
The preparation of tuber of multiflower knotweed holosaccharide: being added the 93-97% ethanol solution of 4-6 times of volume in the concentrate that removing protein is obtained, mixing is equal
Even, precipitates overnight, centrifugation is dry to get tuber of multiflower knotweed refined polysaccharide, due to tuber of multiflower knotweed polysaccharide be it is polar, do not dissolve in ethanol solution, because
Certain density ethanol solution can be added in this in polysaccharide extraction liquid, and polysaccharide forms precipitating, to make polysaccharide and water-soluble monosaccharide
It is separated Deng other substances, obtained tuber of multiflower knotweed refined polysaccharide all has stronger clearance rate to OH, superoxide radical.
Preferably, the mass fraction of removing protein step intermediate ion liquid is 16-20%, ionic liquid is butyl methyl miaow
Azoles tetrafluoroborate [C4mim]BF4, [C4mim]BF4Double-aqueous phase system, most of impurity such as albumen can be formed with many inorganic salts
Matter, minerals and phenolic substances can be extracted in ionic liquid phase, simultaneously [C4mim]BF4It is molten methylene chloride to be extracted to
In liquid, after removing methylene chloride, it can recycle.
Preferably, the mass concentration of inorganic salts is 16-20% in removing protein step, inorganic salts are that weight ratio is 100:
The sodium dihydrogen phosphate and mandelic acid of 1.35-1.45, the weight ratio of L- mandelic acid and D- mandelic acid is 100:3-5, phosphorus in mandelic acid
Acid dihydride sodium system extraction efficiency with higher is suitable as into phase salt, while sodium dihydrogen phosphate and mandelic acid solution system
It is more suitable for being stabilized for tuber of multiflower knotweed polysaccharide with better suited pH range, and sodium dihydrogen phosphate within the scope of the weight ratio and flat
Peach acid content may make polysaccharide to reach most preferably in the extraction efficiency of salt phase, while the inorganic salts can make the pH value of lower phase system
It is lower, and electrostatic repulsion has occurred in the zwitterion of the protein and ionic liquid charged, so that greater protein matter is easy
Be extracted to phase, can be improved the removal rate of protein, at the same D- mandelic acid the antioxygen that can guarantee tuber of multiflower knotweed polysaccharide is added
Change activity.
Compared with the prior art, the advantages of the present invention are as follows: 1) of the present invention tuber of multiflower knotweed polysaccharide have obvious anti-oxidant work
Property, it can be developed into Amti-sanility health-care food auxiliary material or function factor, be applied to food or field of health care products；2) present invention system
Preparation Method is simple, and the extraction efficiency and yield of polysaccharide are higher, can guarantee polysaccharide anti-oxidative activity；3) preparation method of the present invention
Removing protein is carried out using ionic liquid-inorganic salts double-aqueous phase system, mild condition may make polysaccharide to reach in the extraction efficiency of salt phase
To best, process flow is shortened, energy consumption is reduced, reduces consumption of organic solvent, while ionic liquid is available effectively
Recycling and reusing, reduce material consumption, be more suitable for large-scale production and application.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
Tuber of multiflower knotweed polysaccharide, the polysaccharide is by galacturonic acid, galactolipin, arabinose, rhamnose, glucuronic acid and mannose group
At main component is galacturonic acid, accounts for 45.6%, tuber of multiflower knotweed polysaccharide molecular weight range is 100-300kDa, above-mentioned tuber of multiflower knotweed polysaccharide
With obvious antioxidant activity, Amti-sanility health-care food auxiliary material or function factor can be developed into, be applied to food or
Field of health care products.
The preparation method of tuber of multiflower knotweed polysaccharide, including pretreatment of raw material, the preparation of tuber of multiflower knotweed Thick many candies extracting solution, removing protein, the tuber of multiflower knotweed are pure
Polysaccharide preparation, specific preparation step are as follows:
1) pretreatment of raw material: will with petroleum ether by solid-liquid ratio be 1:5 in radix polygoni multiflori powder, temperature is to carry out at degreasing for 45 DEG C of extractions 3 times
Reason, is centrifuged 25rnin at 3,000 rpm after extraction, deposit is dried at 50 DEG C to get degreasing radix polygoni multiflori powder；
2) prepared by tuber of multiflower knotweed Thick many candies extracting solution: being that 1:22 adds water into degreasing radix polygoni multiflori powder by solid-liquid ratio, is 70 DEG C, power in temperature
For ultrasonic extraction 80min under 150W, it is then centrifuged 25rnin at 3,000 rpm, supernatant is concentrated into the 1/4 of original volume, it is dense
Contracting liquid be tuber of multiflower knotweed Thick many candies extracting solution, acted on using ultrasonic wave and generate cavitation effect, form high pressure in histocyte, it is high
The release of pressure forms powerful shock wave in the solution, can effectively reduce, eliminate resistance between water and the tuber of multiflower knotweed, thus plus
Big mass-transfer efficiency, while cell tissue deformation fracture by the physical shear power that shock wave generates, release intracellular organic matter,
Extraction process is greatly accelerated；
3) removing protein: tuber of multiflower knotweed polysaccharide extraction liquid being added in ionic liquid-inorganic salts double-aqueous phase system, mixed system is vibrated to obtain,
Then split-phase 10h is stood at 30 DEG C, will mutually separate up and down, lower phase is fitted into 500kDa bag filter, is dialysed in distilled water
20h is to be concentrated under reduced pressure into 0.25 times of original volume for resulting dialyzate, spare, ion except desalting and other small molecular weight impurities
Liquid-inorganic salts double-aqueous phase system viscosity is smaller, which can be small by protein, minerals in tuber of multiflower knotweed polysaccharide extraction liquid etc.
Molecular impurity is extracted into phase on double-aqueous phase system, and the distribution of tuber of multiflower knotweed polysaccharide recycles lower phase in double-aqueous phase system in lower phase, adds alcohol
The tuber of multiflower knotweed polysaccharide of purifying can be obtained in precipitating, and compared with traditional handicraft, mild condition shortens process flow, reduces energy consumption,
Reduce consumption of organic solvent, while the available effective recycling and reusing of ionic liquid, reduces material consumption, be more suitable for advising greatly
Mould production and application；
4) prepared by tuber of multiflower knotweed holosaccharide: 97% ethanol solution of 4 times of volumes is added in the concentrate that removing protein is obtained, is uniformly mixed,
Precipitates overnight, centrifugation is dry to get tuber of multiflower knotweed refined polysaccharide, due to tuber of multiflower knotweed polysaccharide be it is polar, do not dissolve in ethanol solution, therefore
Certain density ethanol solution can be added in polysaccharide extraction liquid, polysaccharide forms precipitating, to make polysaccharide and water-soluble monosaccharide etc.
The separation of other substances, obtained tuber of multiflower knotweed refined polysaccharide all have stronger clearance rate to OH, superoxide radical.
The mass fraction of above-mentioned removing protein step intermediate ion liquid is 16%, and ionic liquid is butyl methyl imidazoles tetrafluoro boron
Hydrochlorate [C4mim]BF4, [C4mim]BF4Double-aqueous phase system, most of impurity such as protein, mineral can be formed with many inorganic salts
Matter and phenolic substances can be extracted in ionic liquid phase, simultaneously [C4mim]BF4It can be extracted in dichloromethane solution, remove
After removing methylene chloride, it can recycle.
The mass concentration of inorganic salts is 20% in above-mentioned removing protein step, and inorganic salts are the phosphoric acid that weight ratio is 100:1.35
Sodium dihydrogen and mandelic acid, the weight ratio of L- mandelic acid and D- mandelic acid is 100:5 in mandelic acid, sodium dihydrogen phosphate system have compared with
High extraction efficiency is suitable as into phase salt, while sodium dihydrogen phosphate and mandelic acid solution system have better suited pH range
It is more suitable for being stabilized for tuber of multiflower knotweed polysaccharide, and sodium dihydrogen phosphate and almond acid content within the scope of the weight ratio, may make more
Sugar reaches most preferably in the extraction efficiency of salt phase, while the inorganic salts can make the pH value of lower phase system lower, and the albumen charged
Electrostatic repulsion has occurred in the zwitterion of matter and ionic liquid, so that greater protein matter is easy to be extracted to phase, it can
Improve the removal rate of protein, at the same D- mandelic acid be added and can guarantee the antioxidant activity of tuber of multiflower knotweed polysaccharide.
Embodiment 2:
Tuber of multiflower knotweed polysaccharide, the polysaccharide is by galacturonic acid, galactolipin, arabinose, rhamnose, glucuronic acid and mannose group
At main component is galacturonic acid, accounts for 45.6%, tuber of multiflower knotweed polysaccharide molecular weight range is 100-300kDa, above-mentioned tuber of multiflower knotweed polysaccharide
With obvious antioxidant activity, Amti-sanility health-care food auxiliary material or function factor can be developed into, be applied to food or
Field of health care products.
The preparation method of tuber of multiflower knotweed polysaccharide, including pretreatment of raw material, the preparation of tuber of multiflower knotweed Thick many candies extracting solution, removing protein, the tuber of multiflower knotweed are pure
Polysaccharide preparation, specific preparation step are as follows:
1) pretreatment of raw material: will with petroleum ether by solid-liquid ratio be 1:3 in radix polygoni multiflori powder, temperature is to carry out at degreasing for 55 DEG C of extractions 2 times
Reason, is centrifuged 20rnin at 5000rpm after extraction, deposit is dried at 60 DEG C to get degreasing radix polygoni multiflori powder；
2) prepared by tuber of multiflower knotweed Thick many candies extracting solution: being that 1:17 adds water into degreasing radix polygoni multiflori powder by solid-liquid ratio, is 80 DEG C, power in temperature
For ultrasonic extraction 100min under 100W, it then is centrifuged 20rnin at 5000rpm, supernatant is concentrated into the 1/2 of original volume, it is dense
Contracting liquid be tuber of multiflower knotweed Thick many candies extracting solution；
3) removing protein: tuber of multiflower knotweed polysaccharide extraction liquid being added in ionic liquid-inorganic salts double-aqueous phase system, mixed system is vibrated to obtain,
Then split-phase 15h is stood at 20 DEG C, will mutually separate up and down, lower phase is fitted into 350kDa bag filter, is dialysed in distilled water
30h is concentrated under reduced pressure into 0.15 times of original volume with except desalting and other small molecular weight impurities, by resulting dialyzate, spare, above-mentioned
The mass fraction of ionic liquid is 20%, and ionic liquid is butyl methyl tetrafluoroborate [C4mim]BF4, the matter of inorganic salts
Measuring concentration is 16%, and inorganic salts are the sodium dihydrogen phosphate and mandelic acid that weight ratio is 100:1.45, L- mandelic acid and D- in mandelic acid
The weight ratio of mandelic acid is 100:3；
4) prepared by tuber of multiflower knotweed holosaccharide: 93% ethanol solution of 6 times of volumes is added in the concentrate that removing protein is obtained, is uniformly mixed,
Precipitates overnight, centrifugation are dry to get tuber of multiflower knotweed refined polysaccharide.
Embodiment 3:
Tuber of multiflower knotweed polysaccharide, the polysaccharide is by galacturonic acid, galactolipin, arabinose, rhamnose, glucuronic acid and mannose group
At main component is galacturonic acid, accounts for 45.6%, tuber of multiflower knotweed polysaccharide molecular weight range is 100-300kDa, above-mentioned tuber of multiflower knotweed polysaccharide
With obvious antioxidant activity, Amti-sanility health-care food auxiliary material or function factor can be developed into, be applied to food or
Field of health care products.
The preparation method of tuber of multiflower knotweed polysaccharide, including pretreatment of raw material, the preparation of tuber of multiflower knotweed Thick many candies extracting solution, removing protein, the tuber of multiflower knotweed are pure
Polysaccharide preparation, specific preparation step are as follows:
1) pretreatment of raw material: will with petroleum ether by solid-liquid ratio be 1:4 in radix polygoni multiflori powder, temperature is to carry out at degreasing for 50 DEG C of extractions 2 times
Reason, is centrifuged 22rnin at 4000rpm after extraction, deposit is dried at 55 DEG C to get degreasing radix polygoni multiflori powder；
2) prepared by tuber of multiflower knotweed Thick many candies extracting solution: being that 1:20 adds water into degreasing radix polygoni multiflori powder by solid-liquid ratio, is 75 DEG C, power in temperature
For ultrasonic extraction 90min under 120W, it then is centrifuged 22rnin at 4000rpm, supernatant is concentrated into the 1/3 of original volume, it is dense
Contracting liquid be tuber of multiflower knotweed Thick many candies extracting solution；
3) removing protein: tuber of multiflower knotweed polysaccharide extraction liquid being added in ionic liquid-inorganic salts double-aqueous phase system, mixed system is vibrated to obtain,
Then split-phase 12h is stood at 25 DEG C, will mutually separate up and down, lower phase is fitted into 400kDa bag filter, is dialysed in distilled water
For 24 hours with except desalting and other small molecular weight impurities, resulting dialyzate is concentrated under reduced pressure into 0.20 times of original volume, it is spare, it is above-mentioned
The mass fraction of ionic liquid is 18%, and ionic liquid is butyl methyl tetrafluoroborate [C4mim]BF4, the matter of inorganic salts
Measuring concentration is 18%, and inorganic salts are the sodium dihydrogen phosphate and mandelic acid that weight ratio is 100:1.40, L- mandelic acid and D- in mandelic acid
The weight ratio of mandelic acid is 100:4；
4) prepared by tuber of multiflower knotweed holosaccharide: 95% ethanol solution of 5 times of volumes is added in the concentrate that removing protein is obtained, is uniformly mixed,
Precipitates overnight, centrifugation are dry to get tuber of multiflower knotweed refined polysaccharide.
Routine operation in operating procedure of the invention is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (10)
1. tuber of multiflower knotweed polysaccharide, it is characterised in that: the polysaccharide is by galacturonic acid, galactolipin, arabinose, rhamnose, glucose
Aldehydic acid and mannose composition, main component are galacturonic acid.
2. tuber of multiflower knotweed polysaccharide according to claim 1, it is characterised in that: the galacturonic acid accounts for 45.6%.
3. tuber of multiflower knotweed polysaccharide according to claim 1, it is characterised in that: the tuber of multiflower knotweed polysaccharide molecular weight range is 100-
300kDa。
4. the preparation method of tuber of multiflower knotweed polysaccharide, including pretreatment of raw material, the preparation of tuber of multiflower knotweed Thick many candies extracting solution, removing protein, the tuber of multiflower knotweed are pure more
Sugar preparation, it is characterised in that: the removing protein step are as follows: ionic liquid-inorganic salts aqueous two-phase is added in tuber of multiflower knotweed polysaccharide extraction liquid
In system, mixed system is vibrated to obtain, split-phase is stood, will mutually separate up and down, lower phase is packed into bag filter dialysis, by resulting dialysis
Liquid is concentrated under reduced pressure, spare, and the inorganic salts are the mixture of sodium dihydrogen phosphate and mandelic acid.
5. the preparation method of tuber of multiflower knotweed polysaccharide according to claim 4, it is characterised in that: the removing protein step intermediate ion liquid
The mass fraction of body is 16-20%, and the ionic liquid is butyl methyl tetrafluoroborate [C4mim]BF4。
6. the preparation method of tuber of multiflower knotweed polysaccharide according to claim 4, it is characterised in that: removing protein in the removing protein step
The mass concentration of inorganic salts is 16-20% in step, and the inorganic salts are the sodium dihydrogen phosphate that weight ratio is 100:1.35-1.45
And mandelic acid, the weight ratio of L- mandelic acid and D- mandelic acid is 100:3-5 in the mandelic acid.
7. the preparation method of tuber of multiflower knotweed polysaccharide according to claim 4, it is characterised in that: removing protein in the removing protein step
It is 20-30 DEG C that phase separation temperature is stood in step, time 10-15h.
8. the preparation method of tuber of multiflower knotweed polysaccharide according to claim 4, it is characterised in that: the feed pretreatment step are as follows:
To with petroleum ether by solid-liquid ratio be 1:3-5 in radix polygoni multiflori powder, temperature is to carry out ungrease treatment 45-55 DEG C of extraction 2-3 times, extraction terminates
20-25rnin is centrifuged at 3000-5000rpm afterwards, deposit is dried at 50-60 DEG C to get degreasing radix polygoni multiflori powder.
9. the preparation method of tuber of multiflower knotweed polysaccharide according to claim 4, it is characterised in that: the tuber of multiflower knotweed Thick many candies extracting solution system
Standby step are as follows: add water into degreasing radix polygoni multiflori powder by solid-liquid ratio for 1:17-22, in the case where temperature is 70-80 DEG C, power is 100-150W
Then ultrasonic extraction 80-100min is centrifuged 20-25rnin at 3000-5000rpm, supernatant is concentrated into the 1/4 of original volume
To 1/2, concentrate be tuber of multiflower knotweed Thick many candies extracting solution.
10. the preparation method of tuber of multiflower knotweed polysaccharide according to claim 4, it is characterised in that: the tuber of multiflower knotweed holosaccharide preparation step
Suddenly are as follows: the 93-97% ethanol solution of 4-6 times of volume is added in the concentrate for obtaining removing protein, is uniformly mixed, precipitates overnight, from
The heart, it is dry to get tuber of multiflower knotweed refined polysaccharide.
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| CN111544469A (en) * | 2020-05-07 | 2020-08-18 | 成都市明典世家生物科技有限公司 | Method for selectively extracting anthraquinone components in polygonum multiflorum by using ionic liquid solution |
| CN111544469B (en) * | 2020-05-07 | 2022-06-21 | 成都市明典世家生物科技有限公司 | Method for selectively extracting anthraquinone components in polygonum multiflorum by using ionic liquid solution |
| CN111690074A (en) * | 2020-08-04 | 2020-09-22 | 袁静 | Processing equipment for extracting honey peach polysaccharide |
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Application publication date: 20190823 |