CN110151783A - A kind of peritoneal fluid recurred for preventing endometriosis - Google Patents
A kind of peritoneal fluid recurred for preventing endometriosis Download PDFInfo
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- CN110151783A CN110151783A CN201910108621.4A CN201910108621A CN110151783A CN 110151783 A CN110151783 A CN 110151783A CN 201910108621 A CN201910108621 A CN 201910108621A CN 110151783 A CN110151783 A CN 110151783A
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- Prior art keywords
- hydrogen
- endometriosis
- nacl solution
- rich
- recurrence
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/14—Alkali metal chlorides; Alkaline earth metal chlorides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
Abstract
The invention belongs to pharmaceutical preparations technology fields, it is related to clinical gynaecology for preventing the peritoneal fluid of endometriosis recurrence, it is especially that endometriosis operation peritoneal fluid is made in effective component by hydrogen-rich NaCl solution, hydrogen is poured sterile 0.9%NaCl solution using 0.4Mpa pressure by the present invention, it maintains 1 hour, prepare hydrogen-rich NaCl solution, hydrogen concentration is detected with dissolution instrument for analyzing hydrogen, maintain hydrogen concentration 0.5mg/L-1.8mg/L, the hydrogen-rich NaCl solution is for peritoneal irrigation in gynecopathy surgery, the hydrogen-rich NaCl solution improves the oxidative stress status of gynecopathy patient, reduce postoperative recurrence.The recurrence of endometriosis can effectively be prevented.Hydrogen-rich NaCl solution preparation method of the invention is simple, and it is convenient to operate, without side-effects, animal experiment proves that having good effect.
Description
Technical field:
The invention belongs to pharmaceutical preparations technology fields, are related to clinical gynaecology for preventing the abdomen of endometriosis recurrence
Hydrogen-rich NaCl solution is especially that endometriosis operation peritoneal fluid is made in effective component, can had by chamber flushing liquor
The recurrence of effect prevention endometriosis.
Background technique:
Prior art discloses endometriosis (abbreviation gynecopathy) based on dysmenorrhea, chronic pelvic pain, infertile etc.
Symptom is wanted, disease incidence is in increase trend year by year.According to the literature, in dysmenorrhea and the women of chronic pelvic pain, gynecopathy
Disease incidence reaches 40-60%, has drug therapy and operative treatment to the primary treatments of gynecopathy in clinical practice at present,
In, operative treatment is suitable for the patient that symptom is obvious, drug therapy is invalid, has different stove in endometrial cysts or deep, but practices
It shows, the recurrence rate after gynecopathy operative treatment is high, it has been reported that I phase and the postoperative 2 years recurrence rates of II phase gynecopathy
It is 14.3% for 5.7%, III phase and IV phase；Ovary gynecopathy, pelvic peritoneum gynecopathy, deep gynecopathy and ovary merge
Postoperative 4 years recurrence rates of pelvic cavity gynecopathy are respectively 24.6%, 17.8%, 30.6% and 23.7%；Separately have for different in youth
In the research of disease patient, 5 years recurrence rates are up to 56%；Therefore, reducing recurrence rate is the key that improve gynecopathy therapeutic effect.
Currently, clinical be mainly the following the method for reducing gynecopathy recurrence, but there are some drawbacks, such as:
1. Cocktail treatment of performing the operation: such as GnRHa, oral contraceptive, danazol etc., but effect are simultaneously pessimistic, and administration time is long (extremely
It is less 3-6 months), patient compliance decline, while long-term administration can generate low estrogen symptom, osteoporosis, irregularly go out
The side effects such as blood；2. operation joint endometrectomy: it is only applicable to atoke requirement but the firmly patient of Reservations uterus,
And the postoperative caused amenorrhea state of unbearable Endometrectomy on many patients ' psychologicals；3. the postoperative appropriate selection hormone of radical-ability
Replacement therapy (HRT): full uterus adds double accessory resection to can achieve the purpose for eradicating gynecopathy, but this is only applicable to the age
The patient that larger atoke requires, and study discovery for ectopic focus involve peritonaeum > 3cm patient or operation after it is ill
The patient that stove participates in, postoperative HRT treatment not applicable as far as possible.It can be seen that how to reduce gynecopathy Postoperative recurrent rate, it has also become face
The upper more intractable and problem to be solved of bed.
Operation is one of main method of endometriosis, and flushing liquor is a kind of simple and practical treatment side in art
Method, flushing liquor has been used for abdominal cavity adhesion after prevention is performed the operation, prevention of postoperative infection etc. in art at present, but so far, there is not yet can be used for
Reduce the flushing liquor of the recurrence rate of postoperative endometriosis.
Status based on the prior art, the quasi- one kind that provides of present inventor is for preventing endometriosis recurrence
Peritoneal fluid, especially by hydrogen-rich NaCl solution be effective component be made endometriosis perform the operation peritoneal fluid,
The recurrence of endometriosis can effectively be prevented.
Summary of the invention
It is an object of the invention to the statuses based on the prior art, in view of the above-mentioned problems, providing a kind of for preventing uterus
Hydrogen-rich NaCl solution is especially that endometriosis hand is made in effective component by the peritoneal fluid of endometrium ectopia recurrence
Art peritoneal fluid can effectively prevent the recurrence of endometriosis.
The present invention provides a kind of hydrogen-rich peritoneal fluids, can be effective for the recurrence of prevention gynecopathy.Hydrogen is a kind of
Good antioxidant, it is nontoxic, it is easy to get and cheap；Hydrogen has selective antioxidation, can make with oxidation
It is combined with strong free radical, a large number of studies show that, hydrogen-rich water is drunk or injected to animal or human body respiration hydrogen, to eremacausis
Stress disease has apparent therapeutic effect, and the basis based on the prior art, it is effective that the present invention, which uses hydrogen-rich NaCl solution,
Ingredient is made endometriosis operation peritoneal fluid and washes.
The present invention is based on the study found that gynecopathy ectopic endometrium inner membrance and inner membrance in place interstitial cell O2 -、H2O2、 NO
Isoreactivity oxygen (reactive oxygen species, ROS) increases, in addition, superoxide dismutase in Ectopic Endometrium
(superoxide dismutase, SOD) activity increases, and oxidative stress is controlled as a kind of compensatory feedback mechanism, interior different
Lipid oxidation damage markers malonaldehyde (malondialdehyde, MDA) concentration increases in disease patients serum, and and gynecopathy
Be positively correlated by stages, these illustrate gynecopathy patient's body oxidative stress increase, the function of endometrial cell due to ROS and
It changes, including permeability and expression of adhesion molecule, etc., hydrogen-rich peritoneal irrigation is made using hydrogen-rich NaCl solution in the present invention
Liquid, by improving the oxidative stress status of gynecopathy patient, is reduced postoperative multiple for carrying out peritoneal irrigation in gynecopathy surgery
Hair, practice show that hydrogen-rich peritoneal irrigation liquid and preparation method thereof of the invention is simple, and it is convenient to operate, without side-effects.
In the present invention, the endometriosis includes: ovary endometrial cysts, peritonaeum endometriosis,
Deep infiltrative temper Endometriosis, uterus adenomyosis, abdominal cavity other organs endometriosis.
In the present invention, peritoneal fluid is only used for rinsing in laparoscope or abdominal art.
The purpose of the present invention is that technical solution is realized under passing through:
1. preparing hydrogen-rich flushing liquor: preparing hydrogen-rich liquid using the method for increasing pressure, rushed hydrogen using 0.4Mpa pressure
Enter sterile 0.9%NaCl solution, maintain 1 hour, prepares hydrogen-rich NaCl solution；
2. detecting hydrogen molecule concentration: detecting hydrogen concentration with dissolution instrument for analyzing hydrogen, maintain hydrogen concentration 0.5mg/L- 1.8mg/L；
3. implementing gynecopathy operation, after cutting off gynecopathy lesion under abdomen or laparoscope, hydrogen-rich NaCl solution is rinsed into abdomen
Chamber；
4. can multiple lavation, exhaust flushing liquor.
The results show that carrying out peritoneal irrigation in gynecopathy surgery using hydrogen-rich NaCl solution, postoperative recurrence can be reduced,
It is without side-effects.
The present invention provides a kind of for preventing the peritoneal fluid of endometriosis recurrence, especially by hydrogen-rich
NaCl solution is that endometriosis operation peritoneal fluid is made in effective component, is verified by experiments, can effectively prevent uterus
The recurrence of endometrium ectopia.
Detailed description of the invention
A in Fig. 1, the gynecopathy lesion in mouse peritoneal；The gynecopathy lesion that B operation is cut；The gynecopathy disease of C recurrence
Stove.
Fig. 2 shows ImmunohistochemistryResults Results, wherein α-SMA, E-cadherin, p-p65, VEGF are in control group and experimental group
Expression in recurrent focus, is finding under 400 times of mirrors, and scale is 50 μm.
Elaborate below to the embodiment of the present invention: following embodiment under the premise of the technical scheme of the present invention into
Row is implemented, and gives detailed embodiment and process, but protection scope of the present invention is not limited to following embodiments.
Specific embodiment:
Embodiment 1
By endometrium of mouse Endometriosis postoperative recurrence model, the observation present invention is postoperative to prevention endometriosis
The effect of recurrence,
1. experimental animal and grouping: 8 week old Balb/C female mices are randomly divided into control group and medication group；
2. Animal Model:
Mouse establishes model of endometriosis using injection modeling: from modeling the last week, starting to donor mice
It injects oestradiol benzoate (100ug/kg), when modeling, by donor mice conventional treatment, opens stomach wall, take out uterus, donor is small
After mouse uterus shreds and physiological saline is mixed and made into suspension, is injected to the abdominal cavity of Recipient mice, is being hydrated after mouse modeling 2 weeks
It passes through under chloral (300mg/kg) intraperitoneal injection of anesthesia different stove resection in abdomen, cuts off intraperitoneal gynecopathy lesion, make to remain
Lesion is no more than 2, and maximum gauge is less than 0.5mm；
3. preparing hydrogen-rich flushing liquor
1) preparation of hydrogen-rich flushing liquor: hydrogen-rich liquid is prepared using the method for increasing pressure, using 0.4Mpa pressure by hydrogen
Sterile 0.9%NaCl solution is poured, is maintained 1 hour, hydrogen-rich NaCl solution is prepared；
2) it detects hydrogen molecule concentration: detecting hydrogen concentration with dissolution instrument for analyzing hydrogen, maintain hydrogen concentration 0.5mg/L- 1.8mg/L；
4. mouse is divided into 2 groups, one group is control group, and not any processing in art, another group is experimental group, in different in mouse
With the hydrogen-rich NaCl solution lavation abdominal cavity in the present invention in disease operation；
2 weeks execution mouse after operation observe intraperitoneal different stove recurrence, and take lesion to weigh and carry out immunohistochemistry inspection
It surveys；
As the result is shown: the intraperitoneal of all mouse has gynecopathy lesion to recur (as shown in Figure 1), and experimental mice is postoperative
The weight of the interior different stove of recurrence is significantly lower than control group (p < 0.01), and the correlations such as the generation of lesion medium vessels, adherency, fibrosis are exempted from
The expression of epidemic disease group index is below control group (the equal < 0.05 of p value), such as VEGF, E-cadherin, α-SMA, p-p65；Knot
Fruit shows that gynecopathy postoperative recurrence can be effectively reduced using hydrogen-rich NaCl solution lavation abdominal cavity in gynecopathy operation.
Claims (7)
1. a kind of for preventing the peritoneal fluid of endometriosis recurrence, which is characterized in that use hydrogen-rich NaCl solution
For effective component, hydrogen-rich flushing liquor is prepared by following methods；
Hydrogen is poured by sterile 0.9%NaCl solution using 0.4Mpa pressure, maintains 1 hour, prepares hydrogen-rich NaCl solution.
2. according to claim 1 for preventing the peritoneal fluid of endometriosis recurrence, which is characterized in that described
Hydrogen-rich NaCl solution with dissolution instrument for analyzing hydrogen detect hydrogen concentration, maintain hydrogen concentration 0.5mg/L-1.8mg/L.
3. as described in claim 1 or 2 for preventing the peritoneal fluid of endometriosis recurrence, which is characterized in that
The hydrogen-rich NaCl solution improves gynecopathy patient for peritoneal irrigation in gynecopathy surgery, the hydrogen-rich NaCl solution
Oxidative stress status, reduce postoperative recurrence.
4. as described in claim 1 or 2 for preventing the peritoneal fluid of endometriosis recurrence, which is characterized in that
The endometriosis includes: ovary endometrial cysts, peritonaeum endometriosis, deep infiltrative temper Endometrium
Endometriosis, uterus adenomyosis, abdominal cavity other organs endometriosis.
5. purposes of the hydrogen-rich NaCl solution in the peritoneal fluid for being used to prepare prevention endometriosis recurrence.
6. purposes as described in claim 5, which is characterized in that the peritoneal fluid is only used for laparoscope or abdominal
It is rinsed in art.
7. pressing purposes described in claim 5 or 6, which is characterized in that the peritoneal fluid can be used for laparoscope or open abdomen
In surgery after multiple lavation, exhaust flushing liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910108621.4A CN110151783A (en) | 2019-02-02 | 2019-02-02 | A kind of peritoneal fluid recurred for preventing endometriosis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910108621.4A CN110151783A (en) | 2019-02-02 | 2019-02-02 | A kind of peritoneal fluid recurred for preventing endometriosis |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102665707A (en) * | 2009-09-25 | 2012-09-12 | 伊亚索梅股份公司 | N-acetyl-l-cysteine for the treatment of endometriosis |
| CN102836171A (en) * | 2012-09-17 | 2012-12-26 | 北京金康驰医药投资有限公司 | Solution for surgery and endoscope washing and preparation method thereof |
| CN107929314A (en) * | 2016-10-13 | 2018-04-20 | 上海市同济医院 | A kind of hydrogeneous physiological saline and its preparation method and application |
-
2019
- 2019-02-02 CN CN201910108621.4A patent/CN110151783A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102665707A (en) * | 2009-09-25 | 2012-09-12 | 伊亚索梅股份公司 | N-acetyl-l-cysteine for the treatment of endometriosis |
| US20120238627A1 (en) * | 2009-09-25 | 2012-09-20 | Iasomai Ab | N-acetyl-l-cysteine for the treatment of endometriosis |
| CN102836171A (en) * | 2012-09-17 | 2012-12-26 | 北京金康驰医药投资有限公司 | Solution for surgery and endoscope washing and preparation method thereof |
| CN107929314A (en) * | 2016-10-13 | 2018-04-20 | 上海市同济医院 | A kind of hydrogeneous physiological saline and its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| 何嫣: "呼吸高浓度氢气对大鼠子宫内膜异位症的疗效及机制探讨", 《中国优秀硕士学位论文全文数据库》, no. 6, 15 June 2018 (2018-06-15), pages 068 - 6 * |
| 孙学军 等: "《氢分子生物学》", 30 April 2014, 第二军医大学出版社, pages: 152 - 153 * |
| 金继红 等: "腹壁切口瘢痕子宫内膜异位症56例临床分析", 《中国医药导报》, vol. 4, no. 18, 30 June 2007 (2007-06-30), pages 121 * |
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