CN110150338A - A kind of preparation and its application of protectiveness bacteriostatic solution - Google Patents
A kind of preparation and its application of protectiveness bacteriostatic solution Download PDFInfo
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- CN110150338A CN110150338A CN201910553629.1A CN201910553629A CN110150338A CN 110150338 A CN110150338 A CN 110150338A CN 201910553629 A CN201910553629 A CN 201910553629A CN 110150338 A CN110150338 A CN 110150338A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
Abstract
The invention discloses the preparations and its application of a kind of protectiveness bacteriostatic solution, belong to food technology field.It is to mix acquisition with natural bacteriostatic solution using biological wax emulsion as dispersing agent the present invention provides a kind of preparation method of bacteriostatic agent.The solution dispersion stabilization that protective fungicide solution of the invention is formed using biological wax emulsion as substrate is good, and the bacteriostasis time is lasting, and sterilizing rate can achieve 90% or more；Safety and environmental protection can be applied to the fields such as field of food, drug, water process.
Description
Technical field
The present invention relates to the preparations and its application of a kind of protectiveness bacteriostatic solution, belong to food technology field.
Background technique
Microbial contamination refers to the biologic contamination as caused by bacterium and bacteriotoxin, mould and mycotoxin and virus.
Estimate according to the World Health Organization, in the annual hundreds of millions of food origin disease patient in the whole world, 70% is each due to having eaten
Caused by the food and drinking-water of kind invasive organism pollution.
Microbial contamination is also the source of main infection disease.Currently there is 20% respiratory disease by airborne microorganisms
Pollution causes；In the world in most important 41 kinds of great communicable diseases, 14 kinds are caused by the microorganisms spreading in air.Closely
The generation of some big public health events over year, such as SARS, bird flu, hand-foot-and-mouth disease are all related with microbial contamination.
Inhibit the method for microbial contamination to have at present: collection, pre-treatment, processing, transport and storage of raw material etc. are each
Link strict control reinforces the management of environmental sanitation, subtracts as far as possible from various aspects such as the environment of production, equipment and staff
The chance of few microbial contamination, prevents microbial contamination.Also by using some fungicide, bacteriostatic agent etc. to prevent microorganism dirty
Dye, these means are the problem is that larger to the damage of matrix (human body) microflora, and effectively service life is very short,
Sterilization, antibacterial inefficiency.
Summary of the invention
It is using biological wax emulsion as dispersion the first purpose of the invention is to provide a kind of preparation method of bacteriostatic agent
Agent mixes acquisition with natural bacteriostatic solution；The biowax includes but is not limited to beeswax or cane sugar wax；The natural bacteriostatic solution
Including but not limited to mango leaf juice, carrot leaf juice, juice of dandelion.
In one embodiment of the invention, the bacteriostatic agent contains in chitosan, xanthan gum, citric acid and jasmonic
At least one.
In one embodiment of the invention, the bacteriostatic agent contains in starch, Arabic gum, defoaming agent and calcium propionate
At least one.
In one embodiment of the invention, the preparation method includes the following steps: (1) by 20-30 parts of biowaxes
1-3 parts of emulsifiers and 80-100 parts of boiling water are added in melting completely, stir 20-30 minutes, obtain biological wax emulsion；(2) to step
(1) 3-5 parts of natural bactericidal agents, 3-5 parts of chitosans, 1-3 parts of xanthan gum, 1-3 parts of citric acids, 3-5 are added in biological wax emulsion
Part jasmonic, is stirred 10-20 minutes；(3) 5-8 parts of starch, 1-3 parts of Arabic gums, 1-3 parts of defoaming agents, 1-3 parts of propionic acid are added
Calcium stirs 10-20 minutes；(4) 3-5 minutes are stood, it can obtain the protective fungicide solution.
In one embodiment of the invention, the step (1) is to melt biowax at 60~80 DEG C.
In one embodiment of the invention, polysorbate60 or sucrose ester can be selected in the emulsifier in the step (1).
In one embodiment of the invention, DSA-5 defoaming agent can be selected in the defoaming agent in the step (3).
In one embodiment of the invention, described method includes following steps:
(1) preparation of biological wax emulsion: in a kettle by 20-30 parts of biowaxes, raising temperature is to be generated to 60-80 DEG C
Object wax melts completely, and 1-3 parts of emulsifiers, 80-100 parts of boiling water are added, and 3000-5000rpm is stirred 20-30 minutes, forms biology
Wax emulsion；
(2) 3-5 parts of natural bactericidal agents, 3-5 parts of chitosans, 1-3 parts of xanthan are added in the biological wax emulsion of step (1)
Glue, 1-3 part citric acid, 3-5 parts of jasmonics, 1000-3000rpm are stirred 10-20 minutes；
(3) 5-8 parts of starch, 1-3 parts of Arabic gums, 1-3 parts of DSA-5 defoaming agents, 1-3 are added into the solution of step (2)
Part calcium propionate, 1000-3000rpm are stirred 10-20 minutes；
(4) stop stirring, stand 3-5 minutes, it can obtain the protective fungicide solution.
In one embodiment of the invention, the natural bacteriostatic solution the preparation method is as follows: taking dandelion or awns
Fruit leaf or carrot leaf, are cleaned up using clear water, are cut to fritter later, and by it, (10g ginger splices endures 20 in 100g water with Jiang Shui
Minute obtain), starch, honeysuckle be uniformly mixed, squeeze the juice to obtain natural bacteriostatic solution using juice extractor high-speed stirred later, wherein
Jiang Shui: starch: honeysuckle: the mass ratio that dandelion extracts with root (mango, carrot) is 100:3-5:3-5:50-70.
The bacteriostatic agent of the method preparation is also claimed in the present invention.
The utility model has the advantages that
(1) the solution dispersion stabilization that protective fungicide solution of the invention is formed using biological wax emulsion as substrate is good, suppression
Bacterium action time is lasting, does not need using preceding rocking；
(2) material of protective fungicide solution of the invention all selects food grade materials, natural material, safety collar substantially
It protects, can be applied to every field, including field of food, drug field, water treatment field etc.；
(3) protective fungicide solution of the invention is antibacterial, bactericidal effect is excellent, to Escherichia coli, staphylococcus aureus,
The microorganisms such as salmonella have excellent sterilization, fungistatic effect, and sterilizing rate can achieve 90% or more；
(4) protective fungicide solution of the invention has certain film-formation result, effectively using biological wax emulsion as substrate
Ingredient is effectively maintained, therefore antibacterial, bactericidal effect retention time more general fungicide is a little long, reach 15 days with
On；
(5) protective fungicide solution of the invention is easy to use, can pass through the various sides such as spraying, brushing, dipping, coating
Formula is applied on substrate.
Specific embodiment
Bactericidal property test: Escherichia coli, staphylococcus aureus each 10 are added in the medium5CFU/mL, spraying are protected
Shield property germicidal solution, the thalline quantity that test 2h is survived later.Sterilizing rate is
Embodiment 1
The preparation of juice of dandelion: dandelion is extracted with root, and is cleaned up using clear water, is cut to fritter later, by its with
Jiang Shui (10g ginger splices is endured 20 minutes in 100g water and obtained), starch, honeysuckle are uniformly mixed, and use juice extractor high-speed stirring later
It mixes juicing and obtains natural bacteriostatic solution, wherein Jiang Shui: starch: honeysuckle: dandelion=100:5:5:70.
(1) preparation of beeswax lotion:
20 parts of beeswaxs are increased into temperature to 60 DEG C in a kettle, is melted completely to beeswax, 1 part of polysorbate60,80 parts is added
Boiling water, 3000rpm are stirred 30 minutes, form beeswax lotion；
(2) 3 portions of dandelion juice, 3 parts of chitosans, 1 part of xanthan gum, 1 portion of lemon are added in the beeswax lotion of step (1)
Acid, 3 parts of jasmonics, 1000rpm are stirred 20 minutes；
(3) continue to be slowly added into 5-8 parts of cornstarch, 1 part of Arabic gum, 1 part of DSA-5 defoaming agent, 1 part of calcium propionate, it is high
Fast (1000rpm) is stirred 10 minutes；
(4) stop stirring, stand 5 minutes to get protective fungicide solution.
Germicidal solution homogeneity manufactured in the present embodiment is good, places it in supercentrifuge to be centrifuged under the conditions of 1000rmp
Stability test places week age, does not occur precipitating or muddy.
Embodiment 2
The preparation of juice of dandelion: dandelion is extracted with root, and is cleaned up using clear water, is cut to fritter later, by its with
Jiang Shui (10g ginger splices is endured 20 minutes in 100g water and obtained), starch, honeysuckle are uniformly mixed, and use juice extractor high-speed stirring later
It mixes juicing and obtains natural bacteriostatic solution, wherein Jiang Shui: starch: honeysuckle: dandelion=100:4:5:60.
(1) preparation of sucrose wax emulsion:
25 parts of cane sugar waxs are placed in a kettle, temperature is increased to 70 DEG C, is melted completely to cane sugar wax, be added 2 parts and spit
90 parts of boiling water are added in temperature 60, and high speed (4000rpm) stirs 25 minutes, form sucrose wax emulsion.
(2) 4 portions of mango juice, 4 parts of chitosans, 2 parts of xanthan gum, 2 portions of lemons are added in the sucrose wax emulsion of step (1)
Acid, 4 parts of jasmonics, high speed (2000rpm) stir 15 minutes；
(3) continue to be slowly added into 7 parts of potato starch, 2 parts, 2 parts DSA-5 defoaming agents of Arabic gum, 2 parts of calcium propionates, it is high
Fast (2000rpm) is stirred 15 minutes；
(4) stop stirring, stand 4 minutes, it can obtain the protective fungicide solution.It is manufactured in the present embodiment to kill
Bacterium solution uniformity is good, and week age is placed in centrifugal stability test under the conditions of 1000rmp, does not occur precipitating or muddy.
Embodiment 3
The preparation of carrot juice: carrot and carrot leaf are cleaned up using clear water, are cut to fritter later, will
It is uniformly mixed with Jiang Shui (10g ginger splices is endured 20 minutes in 100g water and obtained), starch, honeysuckle, high using juice extractor later
Speed stirring juicing obtains natural bacteriostatic solution, wherein Jiang Shui: starch: honeysuckle: carrot=100:5:5:50.
(1) preparation of beeswax lotion:
30 parts of beeswaxs are placed in a kettle, temperature is increased to 80 DEG C, is melted completely to beeswax, 3 parts of sucrose esters are added,
100 parts of boiling water are added, high speed (5000rpm) stirs 30 minutes, forms beeswax lotion；
(2) 5 portions of carrot juice, 5 parts of chitosans, 3 parts of xanthan gum, 3 portions of lemons are added in the beeswax lotion of step (1)
Acid, 5 parts of jasmonics, high speed (3000rpm) stir 10 minutes；
(3) continue to be slowly added into 8 parts of cornstarch, 3 parts, 3 parts DSA-5 defoaming agents of Arabic gum, 3 parts of calcium propionates, high speed
(3000rpm) is stirred 10 minutes；
(4) stop stirring, stand 3 minutes, it can obtain the protective fungicide solution.
Germicidal solution homogeneity manufactured in the present embodiment is good, and centrifugal stability test is placed one week under the conditions of 1000rmp
Time does not occur precipitating or muddy.
Comparative example 1
The preparation of juice of dandelion: dandelion is extracted with root, and is cleaned up using clear water, is cut to fritter later, by its with
Jiang Shui (10g ginger splices is endured 20 minutes in 100g water and obtained), starch, honeysuckle are uniformly mixed, and use juice extractor high-speed stirring later
It mixes juicing and obtains natural bacteriostatic solution, wherein Jiang Shui: starch: honeysuckle: dandelion=100:5:5:70.
(1) 3 portions of dandelion juice, 3 parts of chitosans, 1 part of xanthan gum, 1 part of citric acid, 3 parts of jasmines are added in 80 parts of boiling water
Jasmine acid, high speed (1000rpm) stir 20 minutes；
(2) continue to be slowly added into 1 part, 1 part 5-8 parts of cornstarch, 1 part of Arabic gum, DSA-5 defoaming agent calcium propionate, it is high
Fast (1000rpm) is stirred 10 minutes；
(3) stop stirring, stand 5 minutes, it can obtain the protective fungicide solution.It is manufactured in the present embodiment to kill
Bacterium solution uniformity is good, and centrifugal stability is tested under the conditions of 1000rmp, and layering will be generated in 1 minute by stopping centrifugation standing, has
Precipitating generates.
Comparative example 2
Do not add natural antimicrobial substances.It is specific the preparation method is as follows:
(1) preparation of sucrose wax emulsion: 25 parts of cane sugar waxs are placed in a kettle, increase temperature to 70 DEG C, to sucrose
Wax melts completely, and 2 parts of polysorbate60s are added, and 90 parts of boiling water are added, and high speed (4000rpm) stirs 25 minutes, forms sucrose wax emulsion；
(2) 2 parts of xanthan gum, 2 parts of citric acids, 4 parts of jasmonics, high speed are added in the sucrose wax emulsion of step (1)
(2000rpm) is stirred 15 minutes；
(3) continue to be slowly added into 7 parts of potato starch, 2 parts, 2 parts DSA-5 defoaming agents of Arabic gum, 2 parts of calcium propionates, it is high
Fast (2000rpm) is stirred 15 minutes；
(4) stop stirring, stand 4 minutes, it can obtain the protective fungicide solution.
Germicidal solution homogeneity manufactured in the present embodiment is good, and centrifugal stability test is placed one week under the conditions of 1000rmp
Time does not occur precipitating or muddy.
Comparative example 3
The preparation of carrot juice: carrot and carrot leaf are cleaned up using clear water, are cut to fritter later, will
It is uniformly mixed with Jiang Shui (10g ginger splices is endured 20 minutes in 100g water and obtained), starch, honeysuckle, high using juice extractor later
Speed stirring juicing obtains natural bacteriostatic solution, wherein Jiang Shui: starch: honeysuckle: carrot=100:5:5:50.
(1) preparation of beeswax lotion: 30 parts of beeswaxs are placed in a kettle, and raising temperature is complete to beeswax to 80 DEG C
Melting, is added 3 parts of sucrose esters, and 100 parts of boiling water are added, and high speed (5000rpm) stirs 30 minutes, forms beeswax lotion；
(2) 5 portions of carrot juice, 5 parts of chitosans, 3 parts of xanthan gum, 3 portions of lemons are added in the beeswax lotion of step (1)
Acid, 5 parts of jasmonics, high speed (3000rpm) stir 10 minutes；
(3) continue to be slowly added into 3 parts of DSA-5 defoaming agents, 3 parts of calcium propionates, high speed (3000rpm) stirs 10 minutes；
(4) stop stirring, stand 3 minutes, it can obtain the protective fungicide solution.
Germicidal solution homogeneity manufactured in the present embodiment is good, when centrifugal stability test is placed 3 days under the conditions of 1000rmp
Between, do not occur precipitating or muddy.
Embodiment 4
It takes 10g bread to do complete sterilization processing, by 0.5g fungicide/100g bread dosage, sprays and protect in bread surface
Shield property germicidal solution, using the method for flat-plate bacterial colony technology respectively to storage 3 days, 5 days, the thalline quantities of 7 days bread surfaces
It is counted.
The bactericidal effect of germicidal solution prepared by Examples 1 to 3 and comparative example 1~3 is measured respectively, the results are shown in Table 1,
Fungistatic effect of the different germicidal solutions of table 1 to bread
Effective storage life: after placing the corresponding time, bactericidal effect is maintained at 90% or more.
10g pork is taken to do complete sterilization processing, by 1g fungicide/100g pork dosage, in pork surface sprayed protection
Property germicidal solution, using flat-plate bacterial colony technology method respectively to storage 3 days, 5 days, the thalline quantity on 7 days pork surfaces into
Row counts.
The bactericidal effect of germicidal solution prepared by Examples 1 to 3 and comparative example 1~3 is measured respectively, the results are shown in Table 2,
Bactericidal effect of the different germicidal solutions of table 2 to pork
It takes 10g banana to do complete sterilization processing, by 0.5g fungicide/100g pork dosage, sprays and protect on banana surface
Shield property germicidal solution, using the method for flat-plate bacterial colony technology respectively to storage 3 days, 5 days, the thalline quantity on 7 days banana surfaces
It is counted.
The bactericidal effect of germicidal solution prepared by Examples 1 to 3 and comparative example 1~3 is measured respectively, the results are shown in Table 3,
Bactericidal effect of the different germicidal solutions of table 3 to banana
From table 1~3 as can be seen that the bactericidal effect of protective fungicide solution prepared by embodiment is significantly larger than commercially available sterilization
Agent, reference examples 1 are not use biological wax emulsion as substrate solution, directly adopt water as substrate solution, therefore stably dispersing
Very poor, the germicidal solution poor effect of property；Reference examples 2 are that natural disinfection solution is not added, and bactericidal effect reduces a part；Control
Example 3 is that potato starch and Arabic gum are not added, and the presence of the two can increase the viscosity of solution, improves the dispersion of solution
Stability, the dispersion of solution is good without before after missing, therefore effect can decline.
Embodiment 5
Using several pieces of bread Jing Guo sterilization processing, (0.5g fungicide is added in 100g bread) sprays 3 × 10 respectively3CFU/
Escherichia coli, staphylococcus aureus, the salmonella of g passes through the bacterium colony of flat-plate bacterial colony technology law test survival after one day
Quantity is calculated by sterilizing rate
As can be seen from Table 4: the germicidal solution of embodiment preparation is to Escherichia coli, staphylococcus aureus and Sharpe door bacterium
There is good bactericidal effect, is higher than commercially available fungicide.
Sterilizing rate of the different germicidal solutions of table 4 to several different bacterium colonies
Embodiment 6
Using several pieces of bread Jing Guo sterilization processing, then sprinkling has 3 × 103The Escherichia coli of CFU/g, golden yellow Portugal
Grape coccus, salmonella quantity ratio 1:1:1 solution (100g bread 1g solution), using place different number of days embodiment with it is right
Germicidal solution as usual carries out sterilization processing, later by the colony counts of flat-plate bacterial colony technology law test survival, passes through sterilization
Rate is calculatedI feels all right to the sequencing of microorganism and fungicide, fungicide and micro- life
After object all sprays, sterilizing rate is tested after 1 day.The sprinkling for carrying out fungicide respectively at the 7th, 15,21 day again, (presses 100g bread
0.5g fungicide is added)
The service life of the different germicidal solutions of table 5
Inventor also verifies the bacteriostatic solution of Examples 1 to 3 preparation to the bacteriostasis property of other microorganisms, ties
Fruit shows that the bacteriostatic solution of Examples 1 to 3 preparation can also effectively inhibit hemolytic streptococcus and Diplococcus pneumopniae, meningitis
The pathogenic microorganisms such as coccus, corynebacterium diphtheriae, pseudomonas aeruginosa, proteus, typhoid bacillus, shigella dysenteriae.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
Claims (10)
1. a kind of preparation method of bacteriostatic agent, which is characterized in that mixed with natural bacteriostatic solution using biological wax emulsion as dispersing agent
It closes and obtains；The biowax includes but is not limited to beeswax or cane sugar wax；The natural bacteriostatic solution includes but is not limited to mango leaf
Juice, carrot leaf juice, juice of dandelion.
2. the method according to claim 1, wherein the bacteriostatic agent contain chitosan, xanthan gum, citric acid and
At least one of jasmonic.
3. according to the method described in claim 2, it is characterized in that, the bacteriostatic agent contain starch, Arabic gum, defoaming agent and
At least one of calcium propionate.
4. any method according to claim 1~3, which is characterized in that the preparation method includes the following steps: (1)
20-30 parts of biowaxes are melted completely, 1-3 parts of emulsifiers and 80-100 parts of boiling water are added, are stirred 20-30 minutes, biology is obtained
Wax emulsion；(2) into the biological wax emulsion of step (1) be added 3-5 parts of natural bactericidal agents, 3-5 parts of chitosans, 1-3 parts of xanthan gum,
1-3 parts of citric acids, 3-5 parts of jasmonics stir 10-20 minutes；(3) be added 5-8 parts of starch, 1-3 parts of Arabic gums, 1-3 parts disappear
Infusion, 1-3 part calcium propionate stir 10-20 minutes；(4) 3-5 minutes are stood, it can obtain the protective fungicide solution.
5. according to the method described in claim 4, it is characterized in that, the step (1) is to melt biowax at 60~80 DEG C.
6. according to the method described in claim 4, it is characterized in that, the emulsifier in the step (1) selects polysorbate60 or sugarcane
Sugar ester.
7. according to the method described in claim 4, it is characterized in that, the defoaming agent in the step (3) is DSA-5 defoaming agent.
8. according to the method described in claim 4, it is characterized in that, described method includes following steps:
(1) preparation of biological wax emulsion: in a kettle by 20-30 parts of biowaxes, temperature is increased to 60-80 DEG C, to biowax
1-3 parts of emulsifiers, 80-100 parts of boiling water are added in melting completely, and 3000-5000rpm is stirred 20-30 minutes, form biowax cream
Liquid；
(2) in the biological wax emulsion of step (1) be added 3-5 parts of natural bacteriostatic solution, 3-5 parts of chitosans, 1-3 parts of xanthan gum,
1-3 parts of citric acids, 3-5 parts of jasmonics, 1000-3000rpm are stirred 10-20 minutes；
(3) 5-8 parts of starch, 1-3 parts of Arabic gums, 1-3 parts of DSA-5 defoaming agents, 1-3 part third are added into the solution of step (2)
Sour calcium, 1000-3000rpm are stirred 10-20 minutes；
(4) stop stirring, stand 3-5 minutes, it can obtain the protective fungicide solution.
9. method according to claim 1 or 8, which is characterized in that the natural bacteriostatic solution the preparation method is as follows: taking
Dandelion or mango leaf or carrot leaf, clean up, are cut to fritter, it is uniformly mixed with Jiang Shui, starch, honeysuckle, high
Speed stirring juicing obtains natural bacteriostatic solution, wherein Jiang Shui: starch: honeysuckle: the matter of dandelion or mango leaf or carrot leaf
Amount is than being 100:3-5:3-5:50-70.
10. the bacteriostatic agent of any the method preparation of claim 1~9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910553629.1A CN110150338A (en) | 2019-06-25 | 2019-06-25 | A kind of preparation and its application of protectiveness bacteriostatic solution |
Applications Claiming Priority (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112369430A (en) * | 2020-11-04 | 2021-02-19 | 青岛翼科新程生物科技有限公司 | Chlorine dioxide nano zinc disinfection and bacteriostasis solution |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902645A (en) * | 2018-06-29 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of plant bacteriostatic agent and preparation method thereof |
| CN109329314A (en) * | 2018-08-30 | 2019-02-15 | 无锡德冠生物科技有限公司 | A kind of raw matter antibacterial agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902645A (en) * | 2018-06-29 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of plant bacteriostatic agent and preparation method thereof |
| CN109329314A (en) * | 2018-08-30 | 2019-02-15 | 无锡德冠生物科技有限公司 | A kind of raw matter antibacterial agent and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369430A (en) * | 2020-11-04 | 2021-02-19 | 青岛翼科新程生物科技有限公司 | Chlorine dioxide nano zinc disinfection and bacteriostasis solution |
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