CN110150327A - A kind of compound biological herbicide and its application method - Google Patents
A kind of compound biological herbicide and its application method Download PDFInfo
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- CN110150327A CN110150327A CN201910526642.8A CN201910526642A CN110150327A CN 110150327 A CN110150327 A CN 110150327A CN 201910526642 A CN201910526642 A CN 201910526642A CN 110150327 A CN110150327 A CN 110150327A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M21/00—Apparatus for the destruction of unwanted vegetation, e.g. weeds
- A01M21/04—Apparatus for destruction by steam, chemicals, burning, or electricity
- A01M21/043—Apparatus for destruction by steam, chemicals, burning, or electricity by chemicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses a kind of compound biological herbicides, including two components of A, B, wherein component A is made of Sclerotium rolfsii SC64, helminthosporium sporangium；B component is made of following raw material: 2-3 parts of Shi Chancao extract, 10-14 parts of organic acid aqueous emulsion, 0.8-1 parts of NSC 619661,0.2-0.3 parts of auxiliary agent, 90-100 parts of ethanol water that mass fraction is 20%.Complex weedicide of the invention includes component A and B component, and component A is that the mycelium of corncob matrix culture reaches weeding purpose by spreading fertilizer over the fields into soil；B component is liquid mist dosage form weeding object, by spray on plants body, achieve the purpose that weeding, is used in combination by the compounding of the two, component A acts on root system, the effect of B component degree plant cell, works along both lines, reaches efficient herbicidal effect, and the two is harmless to crops, it has some improvement to soil tool, meets environmentally friendly, efficient, harmless demand, be with a wide range of applications.
Description
Technical field
The invention belongs to pesticide herbicide technical fields, and in particular, to a kind of compound biological herbicide and its user
Method.
Background technique
Protect crops not by the repetition that the infringement of weeds and other plants for inhibiting crop growth is always in agricultural
Property problem.
In order to help to solve the problems, such as this, the researcher of synthesis chemical field has prepared many different chemistry and has removed
Careless agent, chemical herbicide have the advantages that weeding ratio is high, and still, chemical herbicide, which is used for a long time, has certain harm, first
Herbicide droplet drifted about before this, and the phytotoxicity that the reasons such as the harm for thinking of catabolite cause crop is selectively paid no attention in medical fluid volatilization；
Followed by Reusability herbicide causes the rising of drug resistance weed population, the appearance of antiweed weeds and environmental pollution increasingly
Seriously, so that the difficulty and input of exploitation novel herbicide increasingly increase.To reduce the dependence to chemical herbicide, Ren Mencong
It is extracted in some plants with the active material for inhibiting careless class growth and development, manufactured biological weed killer is with environmental protection, performance
Good feature.
But the biological weed killer using single variety or the single mode of action of long-term continuous high dose be easy to cause it is miscellaneous
The problems such as careless drug resistance and resistance evolution.The reasonable compounding or mixture of herbicide, which have, expands degrass spectrum, improves preventive effect and prolongs
The advantages that generation and development of slow weeds drug resistance and drug resistance, be one of the most effective methods to solve the above problems.
Summary of the invention
The purpose of the present invention is to provide a kind of compound biological herbicide and its application method, including component A and B component, A
Group is divided into microorganism (microbial inoculum class) herbicidal active component, B component include Shi Chancao extract, organic acid aqueous emulsion, auxiliary agent etc. at
Point, component A is that the mycelium of corncob matrix culture reaches weeding purpose by spreading fertilizer over the fields into soil；B component is liquid mist agent
Type weeding object achievees the purpose that weeding by spray on plants body, is used in combination by the compounding of the two, component A makees root system
With the effect of B component degree plant cell works along both lines, reaches efficient herbicidal effect, and the two is harmless to crops, and right
Soil tool has some improvement, and meets modern environmentally friendly, efficient, the harmless demand to herbicide, has a wide range of applications
Prospect.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of compound biological herbicide, including two components of A, B, wherein component A is compacted by Sclerotium rolfsii SC64, standing grain length
Spore bacterium composition；
B component is made of following raw material: 2-3 parts of Shi Chancao extract, 10-14 parts of organic acid aqueous emulsion, coffee
0.8-1 parts of acetoacetic ester, 0.2-0.3 parts of auxiliary agent, 90-100 parts of ethanol water that mass fraction is 20%；
The Shi Chancao extract is prepared by the following method:
(1) stone the moon grass is cleaned to dry in the air in interior to close and is done, 40 DEG C dry to constant weight, and are crushed with medicinal herb grinder, 60 meshes are crossed,
Obtain stone the moon grass meal end；
(2) 250g stone the moon grass meal end is weighed, is extracted 5 times, each 42h with 90% methanol room temperature of 5 times of volumes, is merged and extract
Liquid is concentrated into the 1/4 of original volume with 50 DEG C of Rotary Evaporators, obtains crude extract medicinal extract；
(3) crude extract medicinal extract is heated with methanol, methanol usage be medicinal extract 1/10, on 55 DEG C of water-baths with 70g silicon
Glue mixes well, solvent evaporated, is packed into silica gel column chromatography, and absorbent cotton is successively added and anhydrous sodium sulfate fills cylinder, using second
Acetoacetic ester and petroleum ether are eluted according to 1:6-7, and removed under reduced pressure solvent obtains Shi Chancao extract；
B component is made by the following method: ethanol water is added in Shi Chancao extract, organic acid aqueous emulsion and NSC 619661
In solution, 200r/min stirs 30min, adds auxiliary agent, and first stirring 10min, ultrasound point spreads 10min again, and B component is made；
It is 1:1-2 that the compound biological herbicide, which walks component A and the mass ratio of B component,.
Further, the organic acid aqueous emulsion is made by the following method:
(1) 28g acetone is weighed, keeps 150r/min at the uniform velocity to stir and 25g bamboo acetic acid is added, after adding, continues to keep 150r/
Min, which is at the uniform velocity stirred, is added 5g Tween-80 and 3g ethylene glycol, stirs 25-30min after adding, obtains mixed liquor；
(2) mixed liquor is subjected to homogenization, total 50g deionized water is added portionwise in homogenizing process, until being formed uniform
Shape obtains organic acid aqueous emulsion；
Wherein, the organic acid concentration of bamboo acetic acid is 3.6-7.2%.
Further, the Sclerotium rolfsii SC64 is obtained using corncob as the plate method of fermentation substrate, contains bacterium
The solid fermentation object living bacteria count of silk is 1.88 × 1011mfu/g；Helminthosporium sporangium ferments to obtain using corncob solid matrix
, spore content is 1.96 × 107spores/g。
Further, the auxiliary agent includes ammonium sulfate and triglyceride, and ammonium sulfate and triglyceride are according to mass ratio
1:2 is compounded.
A kind of application method of complex weedicide, includes the following steps:
Step S1, it by soil with tractor rotary tillage 2 times, flattens 1 time, then mechanical trench digging (30-40cm wide, 37-42cm are deep,
It pours water immediately, to fill gully and ponding is not advisable in seedbed；
Step S2, after soil soil table is wet, according to 90kg/hm2Artificial uniformly sowing crops, keep in ditch water level with
Seedbed maintains an equal level；
Step S3, after crop seeding 7-10d, the B component in complex weedicide is applied by spraying using power spraye；
Step S4, after 14-16d, the component A in complex weedicide is spread fertilizer over the fields；
Step S5, after 25-30d, after having spread fertilizer over the fields component A, B component is sprayed immediately, completes weeding.
Beneficial effects of the present invention:
Complex weedicide component A of the invention is microorganism herbicidal active component, compacted using Sclerotium rolfsii SC64, standing grain length
Spore bacterium cooperates to management of weeds, and Sclerotium rolfsii SC64 can effectively prevent and kill off the broadleaf weeds such as water amaranth, Eclipta prostrata and special-shaped Sha
Grass, helminthosporium sporangium can effectively prevent and kill off barnyard grass, and two kinds of being used in compounding for bacteria agent can enhance whole herbicidal effect；It is neat small
Pyrenomycetes SC64 and helminthosporium sporangium can effectively prevent and kill off weeds in field, on the one hand be because moistening for soil, is conducive to mycelia just
Body is activated and is grown from matrix, and infecting plant rapidly keeps its aggrieved, is being kept water level and seedbed in ditch to maintain an equal level, can guaranteed to contain
Water is conducive to rotting, reducing residual for sclerotium；Another aspect helminthosporium sporangium can play during barnyard grass early growth
It is stronger pathogenic, inhibit the growth of barnyard grass；Furthermore containing mycelial corncob solid matrix cheap and easy to get, with bacterium
Silk is manured into soil together, and soil organic matter content can be improved, and is conducive to improve soil texture, promotes crop growth；
B component of the invention includes Shi Chancao extract and organic acid aqueous emulsion, and Shi Chancao extract is to rape, barnyard grass
Root has good inhibiting effect, and the active constituent organic acid of organic acid aqueous emulsion has stronger inhibition to make the growth of weeds
With, meanwhile, the root system of any plant all can naturally secret organic acid, the mineral of nonabsorable around, which is converted to, can be absorbed
Mineral positive ion, so, organic acid be it is natural, be conducive to plant absorption mineral element；Organic acid itself has conditioned soil
The effect of earth；
B component of the invention includes auxiliary component, and the presence of ammonium sulfate can promote plant to herbicide in auxiliary agent
Absorption, plant itself is just very strong to the absorbability of ammonium, and therefore, under the action of ionic pump, hydrogen ion is " pumped " to thin
NH outside after birth4 +It then exchanges into cell membrane, promoting cell membrane external environment is weak acid environment, is conducive to plant cell membrane pair
The infiltration and absorption of weak acid herbicide；During herbicide sprays, due to the presence of wax coat and villus on blade face,
It is often accompanied with medical fluid to be unevenly distributed, influences herbicidal effect, suitable triglycerides, which is added, can be improved medicine drop on blade face
Degree of adhesion, reduce surface tension and contact angle, play drug effect to greatest extent；
Complex weedicide of the invention includes component A and B component, and component A is microorganism (microbial inoculum class) herbicidal active component,
B component includes the ingredients such as Shi Chancao extract, organic acid aqueous emulsion, auxiliary agent, and component A is the mycelium of corncob matrix culture,
By spreading fertilizer over the fields into soil, reach weeding purpose；B component reaches weeding by spray on plants body for liquid mist dosage form weeding object
Purpose is used in combination by the compounding of the two, and component A acts on root system, and the effect of B component degree plant cell is worked along both lines, reached
Efficient herbicidal effect, and the two is harmless to crops, and has some improvement to soil tool, meets the modern times to weeding
Environmentally friendly, efficient, the harmless demand of agent, is with a wide range of applications.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff all other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
A kind of compound biological herbicide, including two components of A, B, wherein component A is compacted by Sclerotium rolfsii SC64, standing grain length
Spore bacterium composition；
Wherein, Sclerotium rolfsii SC64 is obtained using corncob as the plate method of fermentation substrate, hypha-containing solid
Fermentation material living bacteria count is 1.88 × 1011mfu/g；What helminthosporium sporangium was fermented using corncob solid matrix, spore
Content is 1.96 × 107spores/g；Management of weeds is cooperated to using Sclerotium rolfsii SC64, helminthosporium sporangium, it is neat
Pyrenomycetes SC64 can effectively prevent and kill off the broadleaf weeds such as water amaranth, Eclipta prostrata and difformed galingale herb, and helminthosporium sporangium can effectively prevent and kill off barnyard grass,
Two kinds of being used in compounding for bacteria agent can enhance whole herbicidal effect；
B component is made of following raw material: 2-3 parts of Shi Chancao extract, 10-14 parts of organic acid aqueous emulsion, coffee
0.8-1 parts of acetoacetic ester, 0.2-0.3 parts of auxiliary agent, 90-100 parts of ethanol water (mass fraction 20%)；
Wherein, auxiliary agent includes ammonium sulfate and triglyceride, ammonium sulfate and triglyceride according to mass ratio be 1:2 compound and
At；The presence of ammonium sulfate can promote absorption of the plant to herbicide, and plant itself is just very strong to the absorbability of ammonium, because
This, under the action of ionic pump, hydrogen ion is " pumped " to arrive cell membrane NH outside4 +It then exchanges into cell membrane, promotes cell membrane
External environment is weak acid environment, is conducive to infiltration and absorption of the plant cell membrane to weak acid herbicide；It is sprayed in herbicide
In the process, it due to the presence of wax coat and villus on blade face, is often accompanied with medical fluid and is unevenly distributed, influence herbicidal effect,
Suitable triglycerides, which is added, can be improved degree of adhesion of the medicine drop on blade face, reduce surface tension and contact angle, maximum limit
Degree plays drug effect；
The Shi Chancao extract is prepared by the following method:
(1) stone the moon grass is cleaned to dry in the air in interior to close and is done, 40 DEG C dry to constant weight, and are crushed with medicinal herb grinder, 60 meshes are crossed,
Obtain stone the moon grass meal end；
(2) 250g stone the moon grass meal end is weighed, is extracted 5 times, each 42h with 90% methanol room temperature of 5 times of volumes, is merged and extract
Liquid is concentrated into the 1/4 of original volume with 50 DEG C of Rotary Evaporators, obtains crude extract medicinal extract；
(3) by crude extract medicinal extract methanol the 1/10 of medicinal extract (methanol usage be) hot melt, on 55 DEG C of water-baths with 70g silicon
Glue (80-100 mesh) mixes well, solvent evaporated, is packed into silica gel column chromatography (40mm × 600mm, 200-300 mesh silica gel 600g),
Absorbent cotton is successively added and anhydrous sodium sulfate fills cylinder, is eluted, is depressurized according to 1:6-7 using ethyl acetate and petroleum ether
Solvent is removed, Shi Chancao extract is obtained；
The predominantly disconnected connection lignanoids of the chemical component of Shi Chancao, tetrahydrofuran lignanoids, flavonoid glycoside and its aglycon etc.,
After silica gel column chromatography chromatographs, the biggish impurity of polarity therein can be eliminated, then can be with by absorbent cotton, anhydrous sodium sulfate
Lipid therein and moisture are removed, ethyl acetate and petroleum ether can wash away the small impurity of polarity, obtain the higher stone of purity
The moon grass extract, Shi Chancao extract have good inhibiting effect to rape, barnyard grass root；
NSC 619661's (there are lipid structures, easily play a role through phospholipid bilayer), with preferable weeding
Activity and can by influence permeability of cell membranes (plant cell membrane be cell and external environment a boundary, to maintenance
The microenvironment of cell and normal metabolism play an important role, and cell leakage increases, and causes electrolyte inside cell unbalance, in turn
Influence the normal function of cell, cause plant can not normal growth), the content (influencing photosynthesis of plant) and damage of chlorophyll
Leaf Epidermis (destroying Epidermis structure, cause plant that can not carry out normal physiological and biochemical procedure to dead) and inhibit
Weed growth is up to dead, and NSC 619661 has many advantages, such as low toxicity and high security, will not damage to crops；
The organic acid aqueous emulsion is made by the following method:
(1) 28g acetone is weighed, keeps 150r/min at the uniform velocity to stir and 25g bamboo acetic acid is added, after adding, continues to keep 150r/
Min, which is at the uniform velocity stirred, is added 5g Tween-80 and 3g ethylene glycol, stirs 25-30min after adding, obtains mixed liquor；
(2) mixed liquor is subjected to homogenization, total 50g deionized water is added portionwise in homogenizing process, until being formed uniform
Shape obtains organic acid aqueous emulsion；
Wherein, the organic acid concentration of bamboo acetic acid is 3.6-7.2%；Organic acid has stronger inhibition to make the growth of weeds
With, meanwhile, the root system of any plant all can naturally secret organic acid, the mineral of nonabsorable around, which is converted to, can be absorbed
Mineral positive ion, so, organic acid be it is natural, be conducive to plant absorption mineral element；Organic acid itself has conditioned soil
The effect of earth；By carrying out emulsification treatment to organic acid, the stability and dispersibility of organic acid can be improved, improve herbicide medicine
Effect；
Shi Chancao extract, organic acid aqueous emulsion and NSC 619661 are added in ethanol water, 200r/min stirring
30min adds auxiliary agent, and first stirring 10min, ultrasound point spreads 10min again, and B component is made；
Wherein, the mass ratio of component A and B component is 1:1-2；
The application method of the complex weedicide are as follows:
Step S1, it by soil with tractor rotary tillage 2 times, flattens 1 time, then mechanical trench digging (30-40cm wide, 37-42cm are deep,
It pours water immediately, to fill gully and ponding is not advisable in seedbed；
Step S2, after soil soil table is wet, according to 90kg/hm2Artificial uniformly sowing crops, keep in ditch water level with
Seedbed maintains an equal level；
Step S1, after crop seeding 7-10d, the B component in complex weedicide is applied by spraying using power spraye；
Step S3, after 14-16d, the component A in complex weedicide is spread fertilizer over the fields；
Step S4, after 25-30d, after having spread fertilizer over the fields component A, B component is sprayed immediately, completes weeding.
Sclerotium rolfsii SC64 and helminthosporium sporangium can effectively prevent and kill off weeds in field, on the one hand be moistening because of soil,
Just be conducive to mycelium to activate and grow from matrix, infecting plant rapidly keeps its aggrieved, is keeping water level and seedbed in ditch
Maintain an equal level, can guarantee water content, is conducive to rotting, reducing residual for sclerotium；Another aspect helminthosporium sporangium can be in barnyard grass morning
It plays stronger pathogenic in phase growth course, inhibits the growth of barnyard grass；Furthermore contain mycelial corncob cheap and easy to get
Solid matrix is manured into soil together with mycelia, soil organic matter content can be improved, and is conducive to improve soil texture, promote
Crop growth.
Embodiment 1
A kind of compound biological herbicide, including two components of A, B, wherein component A is compacted by Sclerotium rolfsii SC64, standing grain length
Spore bacterium composition；
B component is made of following raw material: 2 parts of Shi Chancao extract, 10 parts of organic acid aqueous emulsion, NSC 619661
0.8 part, 0.2 part of auxiliary agent, 90 parts of ethanol water (mass fraction 20%)；By Shi Chancao extract, organic acid aqueous emulsion and
NSC 619661 is added in ethanol water, and 200r/min stirs 30min, adds auxiliary agent, and first stirring 10min, ultrasound point is spread again
B component is made in 10min；
Wherein, the mass ratio of component A and B component is 1:1.
Embodiment 2
A kind of compound biological herbicide, including two components of A, B, wherein component A is compacted by Sclerotium rolfsii SC64, standing grain length
Spore bacterium composition；
B component is made of following raw material: 2.5 parts of Shi Chancao extract, 12 parts of organic acid aqueous emulsion, caffeic acid second
0.9 part of ester, 0.2-0.3 parts of auxiliary agent, 95 parts of ethanol water (mass fraction 20%)；By Shi Chancao extract, organic sour water
Emulsion and NSC 619661 are added in ethanol water, and 200r/min stirs 30min, add auxiliary agent, first stir 10min and surpass again
Sound point spreads 10min, and B component is made；
Wherein, the mass ratio of component A and B component is 1:1.5.
Embodiment 3
A kind of compound biological herbicide, including two components of A, B, wherein component A is compacted by Sclerotium rolfsii SC64, standing grain length
Spore bacterium composition；
B component is made of following raw material: 3 parts of Shi Chancao extract, 14 parts of organic acid aqueous emulsion, NSC 619661
1 part, 0.3 part of auxiliary agent, 100 parts of ethanol water (mass fraction 20%)；By Shi Chancao extract, organic acid aqueous emulsion and coffee
Coffee acetoacetic ester is added in ethanol water, and 200r/min stirs 30min, adds auxiliary agent, and first stirring 10min, ultrasound point is spread again
B component is made in 10min；
Wherein, the mass ratio of component A and B component is 1:2.
Comparative example 1
Component A.
Comparative example 2
B component.
Comparative example 3
Commercially available biological weed killer.
Performance test is done to the herbicide of embodiment 1-3 and comparative example 1-3, selects corn and soybean field as experimental field,
Under the identical amount of spraying, spraying time and field pipe measure, result is as shown in table 1 below:
Table 1
It is found that the content of 30d corn field weeds is 9.1-9.2%, harvest after herbicide application made from embodiment 1-3
Preceding is 12.5-12.7%；The content of 30d soybean field weeds is 8.2-8.3% after herbicide application made from embodiment 1-3,
It is 10.2-10.4% before harvest, compared to comparative example 1-3, the complex weedicide weeding ratio for illustrating that the present invention is prepared is high,
And can achieve the effect that enhance synergistic in the case where component A and B component compounding, improve the weeding ratio of one-component.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (5)
1. a kind of compound biological herbicide, which is characterized in that including two components of A, B, wherein component A is by Sclerotium rolfsii
SC64, helminthosporium sporangium composition；
B component is made of following raw material: 2-3 parts of Shi Chancao extract, 10-14 parts of organic acid aqueous emulsion, caffeic acid second
0.8-1 parts of ester, 0.2-0.3 parts of auxiliary agent, 90-100 parts of ethanol water that mass fraction is 20%；
The Shi Chancao extract is prepared by the following method:
(1) stone the moon grass is cleaned to dry in the air in interior to close and is done, 40 DEG C dry to constant weight, and are crushed with medicinal herb grinder, cross 60 meshes, obtain
Stone the moon grass meal end；
(2) 250g stone the moon grass meal end is weighed, is extracted 5 times, each 42h with 90% methanol room temperature of 5 times of volumes, combined extract,
It is concentrated into the 1/4 of original volume with 50 DEG C of Rotary Evaporators, obtains crude extract medicinal extract；
(3) crude extract medicinal extract is heated with methanol, methanol usage is the 1/10 of medicinal extract, in filling on 55 DEG C of water-baths with 70g silica gel
Divide and mix thoroughly, solvent evaporated is packed into silica gel column chromatography, and absorbent cotton is successively added and anhydrous sodium sulfate fills cylinder, using acetic acid second
Ester and petroleum ether are eluted according to 1:6-7, and removed under reduced pressure solvent obtains Shi Chancao extract；
B component is made by the following method: ethanol water is added in Shi Chancao extract, organic acid aqueous emulsion and NSC 619661
In, 200r/min stirs 30min, adds auxiliary agent, and first stirring 10min, ultrasound point spreads 10min again, and B component is made；
It is 1:1-2 that the compound biological herbicide, which walks component A and the mass ratio of B component,.
2. a kind of compound biological herbicide according to claim 1, which is characterized in that the organic acid aqueous emulsion is by as follows
Method is made:
(1) 28g acetone is weighed, keeps 150r/min at the uniform velocity to stir and 25g bamboo acetic acid is added, after adding, continues to keep 150r/min
At the uniform velocity 5g Tween-80 and 3g ethylene glycol is added in stirring, stirs 25-30min after adding, obtains mixed liquor；
(2) mixed liquor is subjected to homogenization, total 50g deionized water is added portionwise in homogenizing process, until forming uniform shape, obtained
To organic acid aqueous emulsion；
Wherein, the organic acid concentration of bamboo acetic acid is 3.6-7.2%.
3. a kind of compound biological herbicide according to claim 1, which is characterized in that the Sclerotium rolfsii SC64, with
Corncob be fermentation substrate plate method obtain, hypha-containing solid fermentation object living bacteria count be 1.88 ×
1011mfu/g；What helminthosporium sporangium was fermented using corncob solid matrix, spore content is 1.96 × 107spores/g。
4. a kind of compound biological herbicide according to claim 1, which is characterized in that the auxiliary agent includes ammonium sulfate and sweet
Oily three rouge, ammonium sulfate and triglyceride are that 1:2 is compounded according to mass ratio.
5. a kind of application method of complex weedicide, which comprises the steps of:
Step S1, it by soil with tractor rotary tillage 2 times, flattens 1 time, then (30-40cm wide, 37-42cm are deep, immediately for mechanical trench digging
It pours water, to fill gully and ponding is not advisable in seedbed；
Step S2, after soil soil table is wet, according to 90kg/hm2Artificial uniformly sowing crops, keep water level and seedbed in ditch
Maintain an equal level；
Step S3, after crop seeding 7-10d, the B component in complex weedicide is applied by spraying using power spraye；
Step S4, after 14-16d, the component A in complex weedicide is spread fertilizer over the fields；
Step S5, after 25-30d, after having spread fertilizer over the fields component A, B component is sprayed immediately, completes weeding.
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| CN201910526642.8A CN110150327A (en) | 2019-06-18 | 2019-06-18 | A kind of compound biological herbicide and its application method |
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| CN201910526642.8A CN110150327A (en) | 2019-06-18 | 2019-06-18 | A kind of compound biological herbicide and its application method |
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