CN110141397B - Euthanasia device of experimental animals - Google Patents
Euthanasia device of experimental animals Download PDFInfo
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- CN110141397B CN110141397B CN201910460667.2A CN201910460667A CN110141397B CN 110141397 B CN110141397 B CN 110141397B CN 201910460667 A CN201910460667 A CN 201910460667A CN 110141397 B CN110141397 B CN 110141397B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
- A61D7/04—Devices for anaesthetising animals by gases or vapours; Inhaling devices
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Abstract
The invention discloses a euthanasia device for experimental animals, which comprises: the device comprises a frame, a bin, a bottom box and an asphyxiant gas inlet valve, wherein the frame is provided with a plurality of bins, the bottom box can be inserted into the bins in a drawer mode, the side wall of each bin is provided with an oppressive depression bar air release valve, an oppressive depression bar air inlet valve, an oppressive depression bar air charging source and an asphyxiant gas inlet valve, the top of each bin is provided with an oppressive base plate, an inflatable oppressive depression bar is arranged below the oppressive depression base plate, the oppressive depression base plate is provided with a multi-way connector, one end of the multi-way connector is connected with the oppressive depression bar air charging source, the other end of the multi-way connector is connected with the oppressive depression bar air charging; the invention provides the euthanasia device for the experimental animal, which is good in tightness, safe, intelligent and small in stimulation to the experimental animal.
Description
Technical Field
The invention relates to the field of animal experiments, in particular to a euthanasia device for experimental animals.
Background
In the field of animal experiments, when the relative permission and qualification of an experimental animal institution are evaluated, the institution examines and verifies the disposal mode of rejected experimental animals as a hard index. The experimental animal mechanism is divided into a use type mechanism and a production type mechanism according to different working properties. The animal elimination amount of a production type mechanism reaches hundreds or even thousands of animals every day, the scale of a use type mechanism is rapidly increased year by year with the attention of China to the life science field, and more experimental animal use mechanisms with the scale of tens of thousands of cages are used at present. With the increase of the usage amount of animals, animals needing to be subjected to euthanization are more and more, and the conventional operation of euthanization on small-batch objects cannot meet the requirements of production type experimental animal institutions.
The small animal euthanization devices generally adopted at present are divided into manual devices and automatic devices.
The manual equipment is basically composed of an openable sealed organic glass container, the top of the container is provided with a gas pipe connector, and a carbon dioxide steel cylinder is directly connected to the connector through a gas pipe. During operation, the animals are directly placed in the container, the gas cylinder valve is opened, and carbon dioxide is continuously filled until the animals stop moving. However, the manual device has the following drawbacks: the manual equipment is basically operated in a non-concentration control and over-inflation mode, the method runs counter to relevant regulations of welfare of experimental animals, on one hand, certain pain is caused to the animals in the treatment process, on the other hand, uncertainty of treatment results is caused by the randomness of personnel operation in the treatment process, and the possibility that the animals only enter a pseudo-death shock state and life-saving escape journey biological safety accidents occur after a period of time; or biological sample pollution caused by contacting with carbon dioxide with too high concentration influences experimental data and the like. In addition, the gas in the sealed container cannot be discharged in an organized manner, and secondary damage can be caused to operators.
Automatic equipment has various types from low automation degree to high automation degree, the most basic type only uses the carbon dioxide gas inlet valve and the carbon dioxide gas release valve to carry out automatic control, control elements only comprise the opening and closing duration of the valve, some operation parameters are stored, and some safety protection measures are added, such as the detection of a cabin door opening and closing and an equipment power supply, so that the carbon dioxide gas cannot be leaked. The most advanced automatic equipment at present can carry out the short-term test to the concentration of carbon dioxide to according to the on-off time of concentration digital control carbon dioxide valve, be equipped with some safety protection measures simultaneously, organize the mechanism that carbon dioxide gas concentrates to discharge to and ultraviolet disinfection function, utilize the pressure rising board to keep the function of inner chamber negative pressure when sealing the gas filled to the container of multiple specification. However, the automation device has the following disadvantages: although the most advanced existing automation equipment can theoretically stabilize the operation process by controlling the concentration parameter, the most advanced automation equipment has certain defects in practical use. For example, in actual use, a carbon dioxide high-pressure steel cylinder is used for supplying gas to equipment, and since the gas is elastic, and the pressure change of the carbon dioxide is huge when the pressure of the carbon dioxide is reduced from a high-pressure state to a normal-pressure state, the gas filled in the box is often not accurate only by using a method for detecting concentration in the gas supply process, and in the actual use process, if a valve is large, the concentration change is very fast, so that the gas is often filled too much. In some literature on animal welfare, it is also pointed out that the value of the carbon dioxide inflow should be controlled within a range in which the experimental animal is subjected to euthanasia, otherwise the experimental animal is subjected to pain. The old-model automatic equipment can only carry out the euthanasia operation to the experimental animal of a container at every turn, and operating efficiency is lower, to large-scale experimental animal use mechanism and experimental animal production mechanism, can not satisfy daily animal handling volume. In addition, although the lifting pressing plate mechanism of old-model automation equipment can adapt to cage boxes with different specifications, the pressing plate mechanism is complex in structure, consists of a plurality of components such as crossed levers, reset springs, limit sensors and driving motors, and is high in cost and inconvenient to install and maintain. In animal welfare regulations, the whole process of euthanasia of experimental animals should be observed to avoid the phenomenon which is contrary to animal welfare in the process of disposal, but although the old model of equipment is provided with an observation window, the brightness of the cabin in the equipment is low to avoid stimulation to the animals in the container, which leads to the situation that operators can hardly observe the experimental animals clearly.
Disclosure of Invention
In order to solve the technical problems, the invention provides the euthanasia device for the experimental animal, which has good tightness, safety, intelligence and small stimulation to the experimental animal, the device comprises a frame, a bin, a bottom box and a asphyxia gas inlet valve, wherein a plurality of bins are arranged on the frame, the bottom box can be inserted into the bin in a drawer mode, the side wall of the bin is provided with a stuffy depression bar air release valve, a stuffy depression bar air inlet valve, a stuffy depression bar air inflation source and a suffocation gas inlet valve, the top of the bin is provided with a stuffy pressing base plate, an inflatable stuffy pressing bar is arranged below the stuffy pressing base plate, the stuffy pressing base plate is provided with a multi-way joint, one end of the multi-way joint is connected with the stuffy pressing strip inflation source, the other end of the multi-way joint is connected with the stuffy pressing strip air inlet valve, the other end of the multi-way joint is connected with the stuffy pressing strip deflation valve, the inflatable stuffy pressing strip is inflated by the stuffy pressing strip inflation source through the multi-way joint, and asphyxiant gas is input into the bottom box through the asphyxiant gas inlet valve;
in one state, the bottom box is arranged in the bin position, the stuffy pressing substrate is arranged above the bottom box, and the stuffy pressing substrate and the inflatable stuffy pressing strip between the bottom boxes can reinforce the tightness of the stuffy pressing substrate and the bottom box.
The inner cavity of the euthanasia device is designed into a structure with multiple bin positions, and the euthanasia program can be independently carried out, so that the euthanasia device is convenient to control.
Preferably, the euthanasia device for the experimental animal provided by the invention further comprises a stuffy pressing strip pressure detection switch, the stuffy pressing strip pressure detection switch is arranged on the side wall of the bin position, one end of the multi-way joint is connected with the stuffy pressing strip pressure detection switch, the pressure in the stuffy pressing strip is detected through the stuffy pressing strip pressure detection switch, after the pressure reaches a preset value, the stuffy pressing strip air inlet valve and the stuffy pressing strip inflation source are closed, the pressure in the stuffy pressing strip is continuously kept, and if the pressure is detected to be lower than the preset value, the stuffy pressing strip air inlet valve and the stuffy pressing strip inflation source are controlled to; an inflatable stuffy pressing strip is designed and fixed on a stuffy pressing base plate, after inflation, the stuffy pressing strip locally expands downwards, and a circle of sealing ring is formed at the top of a special bottom box by utilizing the end face, so that the sealing property in the box is ensured. And a sealing strip pressure detector is added, and the air is inflated again when the pressure is insufficient under the control of a program, so that the sealing property is enhanced. The pressure tight effect is ideal under the actual test, and simple structure moreover, the occupation space is little, and the cost is lower.
Preferably, the euthanasia device of the experimental animal provided by the invention further comprises a suffocation gas regulating valve and a suffocation gas flow rate detecting meter, wherein a certain amount of suffocation gas is input into the bottom box through the suffocation gas regulating valve and the suffocation gas flow rate detecting meter; the design can accurately provide the required asphyxiating gas.
Preferably, the euthanasia device for the experimental animals further comprises a harmless gas inlet channel, a harmless gas outlet channel and a suction device, wherein the harmless gas inlet channel is arranged beside the side frame of the frame, the harmless gas outlet channel is arranged in the frame, and the suction device is arranged at the tail end of the harmless gas outlet channel; the harmless gas outlet channel generates continuous unidirectional airflow through the air suction device, the gas in the bin is sucked out, and the sucked gas is replenished through the harmless gas inlet channel, so that the bin is maintained in a state of negative pressure flow to the outside; the two sides of the device are provided with air collecting channels, and the middle of the equipment is provided with an air suction channel at intervals, so that one-way airflow in the cabin is maintained, and the asphyxiating gas in the bottom box can not be dispersed outside the equipment to influence operators if the leakage occurs.
Preferably, the euthanasia device for experimental animals provided by the invention further comprises a harmless gas inlet valve, the harmless gas inlet valve is arranged on the side wall of the bin, harmless gas can be injected into the bottom box through the harmless gas inlet valve, and the harmless gas injected into the bottom box flows out along the harmless gas outlet channel.
Preferably, the euthanasia device for experimental animals provided by the invention further comprises an electrical appliance control bin and an operation interface, wherein the electrical appliance control bin and the operation interface are arranged on the upper side of the frame, the electrical appliance control bin comprises a power supply processing module, a data acquisition processing module and an equipment ventilation power module, the data acquisition processing module is used for acquiring data of the working state of the device, the ventilation power module is used for controlling the inlet and outlet of harmless gas, the operation interface is used for providing an interface of the control device for a user, and the power supply processing module is used for supplying power to the data acquisition processing module, the equipment ventilation power module and the operation interface; a small amount of experimental animals need to be treated, and a small container can be placed in the special bottom box. Because the volume of the bottom box is fixed, the flow of the charged carbon dioxide can also be set to be fixed, only the regulating valve and the flow detector are needed to be arranged in the original air inlet pipeline, the flow of the charged carbon dioxide can meet the relevant animal welfare standards, and the digitized data is convenient to operate and record.
Preferably, the euthanasia device for experimental animals provided by the invention further comprises a night vision camera and an image display, wherein the night vision camera is arranged at the top of the pressure tight substrate, the image display is arranged at the top of the frame, and the night vision camera monitors information in the bottom box and transmits the information to the image display; the night vision camera at the top can clearly reflect the image in the bottom box in the display for the operator to observe conveniently and clearly.
Preferably, the bottom case is made of opaque ABS material, and the wall thickness is 3-4 mm.
Preferably, the asphyxiating gas comprises carbon dioxide.
Preferably, the non-hazardous gas comprises ordinary air.
Preferably, an LED illuminating lamp is arranged in the bin, the LED lamp can enable the inside of the bottom box to generate light transmittance with certain brightness, and animals cannot be stimulated by too strong light.
Preferably, a suffocation gas concentration detection module and an alarm module are arranged outside the frame, and when the concentration of the suffocation gas in the environment detected by the suffocation gas concentration detection module is higher than a preset value, the alarm module is triggered to alarm; the alarm module is still operational even when the euthanasia device is turned off.
The use method of the euthanasia device for the experimental animals, provided by the invention, is briefly summarized as the following steps:
1. placing a bottom box containing the experimental animal to be treated in a bin;
2. the stuffy pressing strip inflation source is used for inflating the stuffy pressing strip, and the bottom box is sealed relative to the bin position;
3. the bottom box is filled with the asphyxiant gas through the asphyxiant gas inlet valve, and the harmless gas outlet channel generates continuous one-way airflow through the air suction device to suck out the gas in the bin;
4. after the experimental animal to be treated is euthanized, harmless gas is injected into the bottom box through the harmless gas inlet valve, and the harmless gas injected into the bottom box and the residual asphyxiating gas in the bottom box flow out along the harmless gas outlet channel.
Compared with the prior art, the technical scheme of the invention has the beneficial effects that: the invention provides the euthanasia device for the experimental animal, which has good tightness, safety and intelligence and small stimulation to the experimental animal; the safety performance of the device is improved through the design of the stuffy pressing base plate, the inflatable stuffy pressing strip and the like, and the effective concentration of the asphyxia gas is also ensured; through the design of the bottom box, the night vision camera and the like, the experimental animal is prevented from being stimulated by strong light, and the pain of the experimental animal is reduced.
Drawings
FIG. 1 is a schematic structural diagram of a euthanasia device for experimental animals according to an embodiment of the present invention;
FIG. 2 is a schematic view of a partial structure of a euthanasia device for experimental animals according to an embodiment of the present invention;
FIG. 3 is a schematic view of a partial structure of a euthanasia device for experimental animals according to an embodiment of the present invention;
in the drawings: 1. the device comprises a frame, 2, a bin position, 3, a bottom box, 4, a stuffy depression bar air release valve, 5, a stuffy depression bar air inlet valve, 6, a stuffy depression bar air charging source, 7, a stuffy depression bar pressure detection switch, 8, a harmless gas air inlet valve, 9, a stuffy depression base plate, 10, an air charging stuffy depression bar, 11, a harmless gas inlet channel, 12, a harmless gas outlet channel, 13, an electric appliance control bin, 14, an operation interface, 15, an image display, 16, a universal wheel, 17 and a suffocation gas inlet channel.
The specific implementation mode is as follows:
the invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1 and fig. 2, a structural diagram of a euthanasia device of an experimental animal is shown, wherein the euthanasia device comprises a frame 1, a bin 2, a bottom box 3, a tight pressing strip air release valve 4, a tight pressing strip air inlet valve 5, a tight pressing strip air charging source 6, a tight pressing strip pressure detection switch 7, a harmless gas inlet valve 8, a tight pressing base plate 9, an air charging tight pressing strip 10, a harmless gas inlet channel 11, a harmless gas outlet channel 12, an electrical appliance control bin 13, an operation interface 14, a video display 15, a universal wheel 16 and an asphyxiant gas inlet channel 17, the frame 1 is provided with a plurality of bins 2, in the embodiment, the bins 2 are totally 8 and are arranged by 2 x 4, the bottom box 3 can be inserted into the bin 2 in a drawer manner, the side wall of the bin 2 is provided with a tight pressing strip air release valve 4, a tight pressing strip air inlet valve 5, a tight pressing strip air charging source 6 and an asphy, an inflatable stuffy pressing strip 10 is arranged below the stuffy pressing base plate 9, an inflatable source 6 of the stuffy pressing strip inflates the inflatable stuffy pressing strip 10 through an inlet valve 5 of the stuffy pressing strip, and asphyxiant gas is input into the bottom box 3 through an inlet valve 17 of the asphyxiant gas; when the bottom box 3 is arranged in the bin 2, the stuffy pressing base plate 9 and the bottom box are sealed through the inflating stuffy pressing strip 10. The stuffy pressing strip pressure detection switch 7 is arranged on the side wall of the bin 2, and the stuffy pressing strip pressure detection switch 7 detects the pressure of the inflating stuffy pressing strip 10. A harmless gas inlet channel 11 is arranged beside the side frame of the frame 1, a harmless gas outlet channel 12 is arranged in the frame 1, and a gas suction device (not shown in the drawing) is arranged at the tail end of the harmless gas outlet channel 12; the harmless gas outlet channel 12 generates continuous one-way airflow through the air suction device to suck the gas in the bin 2, and the sucked gas is complemented by the harmless gas inlet channel 11, so that the bin 2 is maintained in a state of negative pressure flow to the outside. The harmless gas inlet valve 8 is arranged on the side wall of the bin 2, the harmless gas can be injected into the bottom box 3 through the harmless gas inlet valve 8, and the harmless gas injected into the bottom box 3 flows out along the harmless gas outlet channel 12. Electrical apparatus control storehouse 13, operation interface 14 set up in frame 1 upside, electrical apparatus control storehouse 13 includes power supply processing module, data acquisition processing module, the power module that takes a breath of equipment, and data acquisition processing module carries out data acquisition to device operating condition, and the power module that takes a breath controls harmless gas and advances/goes out, and operation interface provides controlling means's interface for the user, and power supply processing module is data acquisition processing module, the power module that takes a breath of equipment, operation interface power supply. The night vision camera (not shown in the figure) is arranged on the top of the pressure tight substrate 9, the image display 15 is arranged on the top of the frame 1, and the night vision camera monitors information in the bottom box 3 and transmits the information to the image display 15.
In this embodiment, the bottom case is made of opaque ABS material and has a wall thickness of 4 mm.
In this embodiment, the asphyxiating gas is carbon dioxide.
In this embodiment, the harmless gas includes ordinary air.
In this embodiment, a suffocation gas concentration detection module and an alarm module are arranged outside the frame 1, and when the concentration of the suffocation gas in the environment detected by the suffocation gas concentration detection module is higher than a preset value, the alarm module is triggered to alarm.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.
Claims (9)
1. A device for euthanizing a laboratory animal, comprising: the device comprises a frame, a bin, a bottom box and an asphyxiant gas inlet valve, wherein the frame is provided with a plurality of bins, the bottom box can be inserted into the bins in a drawer mode, the side wall of each bin is provided with an oppressive depression bar air release valve, an oppressive depression bar air inlet valve, an oppressive depression bar air inflation source and an asphyxiant gas inlet valve, the top of each bin is provided with an oppressive base plate, an oppressive depression bar is arranged below each oppressive base plate, the oppressive depression bar base plate is provided with a multi-way connector, one end of each multi-way connector is connected with the corresponding oppressive depression bar air inflation source, the other end of each multi-way connector is connected with the corresponding oppressive depression bar air inlet valve, the other end of each oppressive depression bar air;
in one state, the bottom box is arranged in the bin position, the stuffy pressing substrate is arranged above the bottom box, and the air inflation stuffy pressing strip between the stuffy pressing substrate and the bottom box can reinforce the tightness of the stuffy pressing substrate and the bottom box;
the frame is characterized by further comprising a harmless gas inlet channel, a harmless gas outlet channel and a gas suction device, wherein the harmless gas inlet channel is arranged beside the side frame of the frame, the harmless gas outlet channel is arranged in the frame, and the gas suction device is arranged at the tail end of the harmless gas outlet channel;
the harmless gas outlet channel generates continuous unidirectional airflow through the air suction device, the gas in the bin is sucked out, the sucked gas is replenished through the harmless gas inlet channel, and the bin is maintained in a state of negative pressure flow to the outside.
2. The euthanasia device for laboratory animals according to claim 1, further comprising
The stuffy pressing strip pressure detection switch is arranged on the side wall of the bin position, one end of the multi-way joint is connected with the stuffy pressing strip pressure detection switch, the stuffy pressing strip pressure detection switch is used for detecting the internal pressure of the stuffy pressing strip, after the pressure reaches a preset value, the stuffy pressing strip air inlet valve and the stuffy pressing strip air inflation source are closed, the internal pressure of the stuffy pressing strip is continuously kept, and if the pressure is detected to be lower than the preset value, the stuffy pressing strip air inlet valve and the stuffy pressing strip air inflation source are controlled to be inflated again.
3. A euthanasia apparatus for laboratory animals according to claim 1, further comprising a asphyxia gas adjusting valve and a asphyxia gas flow rate detector, wherein a specific amount of asphyxia gas is supplied to the bottom case through the asphyxia gas adjusting valve and the asphyxia gas flow rate detector.
4. The experimental animal euthanasia device according to claim 1, further comprising a harmless gas inlet valve disposed on the side wall of the bin,
the harmless gas can be injected into the bottom box through the harmless gas inlet valve, and the harmless gas injected into the bottom box flows out along the harmless gas outlet channel.
5. The experimental animal euthanasia device of claim 4, further comprising an electrical control cabin and an operation interface, wherein the electrical control cabin and the operation interface are arranged on the upper side of the frame, the electrical control cabin comprises a power supply processing module, a data acquisition and processing module and an equipment ventilation power module, the data acquisition and processing module performs data acquisition on the working state of the device, the ventilation power module controls the harmless gas to enter and exit, the operation interface provides an interface of a control device for a user, and the power supply processing module supplies power to the data acquisition and processing module, the equipment ventilation power module and the operation interface.
6. The euthanasia device for laboratory animals as claimed in claim 1, further comprising a night vision camera disposed on top of said hermetically sealed substrate, and an image display disposed on top of said frame, wherein said night vision camera monitors information in said bottom case and transmits said information to said image display.
7. The euthanasia device for laboratory animals as claimed in claim 1, wherein the bottom case is made of opaque ABS material, the wall thickness is 3-4mm, and an LED lamp is arranged in the bin and can enable the inside of the bottom case to generate light transmittance with certain brightness.
8. A device as claimed in claim 4, wherein said asphyxiating gas comprises carbon dioxide; the harmless gas comprises ordinary air.
9. The euthanasia device for experimental animals as claimed in claim 1, wherein a asphyxia gas concentration detection module and an alarm module are arranged outside the frame, and when the concentration of the asphyxia gas in the environment detected by the asphyxia gas concentration detection module is higher than a preset value, the alarm module is triggered to alarm.
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| CN201910460667.2A CN110141397B (en) | 2019-05-30 | 2019-05-30 | Euthanasia device of experimental animals |
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| CN201910460667.2A CN110141397B (en) | 2019-05-30 | 2019-05-30 | Euthanasia device of experimental animals |
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| CN110141397B true CN110141397B (en) | 2021-05-11 |
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