CN110140807A - A kind of feed addictive reducing egg yolk cholesterol - Google Patents
A kind of feed addictive reducing egg yolk cholesterol Download PDFInfo
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- CN110140807A CN110140807A CN201910537903.6A CN201910537903A CN110140807A CN 110140807 A CN110140807 A CN 110140807A CN 201910537903 A CN201910537903 A CN 201910537903A CN 110140807 A CN110140807 A CN 110140807A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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Abstract
The present invention provides a kind of feed addictive that can reduce egg yolk cholesterol, including following parts by weight component: 40-50 parts of alpha-linolenic acid, 20-30 parts of conjugated linoleic acid, 15-20 parts of green tea powder, 10-15 parts of mulberry leaf powder, 1-2 parts of curcumin, 0.5-1 parts of stilbene grass extract, 1-2 parts of Mao Xu Cao extract, 0.05-0.1 parts of alfalfa saponin.The feed addictive prescription that the present invention can reduce egg yolk cholesterol passes through the absorption (alfalfa saponin of reduction endogenous cholesterol, mulberry leaf powder), influence the activity (curcumin of expression (the stilbene grass extract) and relevant enzyme of laying hen cholesterol metabolic relevant enzyme or gene, mulberry leaf powder, CLA, ALA), and then interfere the synthesis and metabolism of cholesterol, furthermore, material composition green tea powder, mulberry leaf powder, curcumin, Mao Xu Cao extract has very strong antioxidant activity, protect CLA, ALA is not oxidized, to guarantee the performance of its effect, it is finally reached the purpose for reducing egg yolk cholesterol level.
Description
Technical field
The invention belongs to field of feed, and in particular to one kind can reduce egg yolk cholesterol feed addictive and
Its application method.
Background technique
Egg nutrient is abundant, for a long time by the favor of people.However since last century, there is scholar to propose egg gallbladder
Sterol content is relatively high, and intake egg will lead to human serum cholesterol and increase too much, and then cause cardiovascular disease.But
Also it is believed that, the intake of moderate egg will not generate any influence to serum cholesterol level.This arguement continues up
Till now, so that many people, the especially middle-aged and the old with cardiovascular disease dare not often eat egg.
Cholesterol mainly from exogenous absorption and is endogenously synthesized in laying hen body.Exogenous absorption is mainly from feeding
Material, is endogenously synthesized mainly from liver.For bird inlay, liver and ovary are the major organs of synthetic cholesterol, but
Liver is not only the organ that aggregate velocity is most fast and synthetic quantity is most, is gallbladder in the most active synthesising part of body and blood plasma
The main source of sterol.Cholesterol biosynthesis is the process of a multistep enzymatic reaction, the 3-hydroxy-3-methylglutaric acid in liver
Single acyl coenzyme A reductase (HMGR) is the rate-limiting enzyme of cholesterol biosynthesis, decides the speed of reaction, controls the life of free cholesterol
Object synthesis.Cholesterol for carrier, is synthesized under the assistance of various transport factors and is released from liver with apo-B (Apo-B)
It puts, eventually arrives at the egg mother cell in growth, free cholesterol is decomposed under the action of lysosome, finally in a free form
With the growth of egg mother cell, finally make cholesterol deposition in yolk.Very low density lipoprotein receptor (VLDLR) is in ovary
The serum total cholesterol (TC) of the main carriers of laying hen blood cholesterol levels, storage reaches Oocytes after transporting, and passes through
Acceptor medium egg cell yolk generates the encytosis of receptor (OVR), is absorbed by the egg mother cell in growth, ultimately forms yolk
In 95% cholesterol.By inhibiting the expression of the enzyme (HMGR) and the gene (VLDLR), have to cholesterol level of vitellus is reduced
The effect of highly significant.HMGR is the substrate specificity of the protein kinase (AMPK) of adenosine monophosphate activation, and AMPK passes through phosphorylation
Ser871 makes its inactivation in HMGR peptide chain, inhibits cholesterol biosynthesis.Cholesterol is mainly cell in the intracorporal operation process of laying hen
Inner cholesterol recombines, assembles and enter by receptor-mediated cholesterol absorption the process of egg mother cell.Therefore, all to gallbladder
Key factor during absorption, synthesis, metabolism, operating, deposition of sterol etc. is influential, all may be realization artificial adjustment
The important target spot that cholesterol deposits in egg mother cell.
Patent CN104642811B discloses a kind of egg feedstuff for reducing cholesterol in egg, in terms of mass fraction, by
615~680 parts of corn, 28~32 parts of wheat bran, 190~210 parts of Soybean Meal, 83~92 parts of mountain flour, 12~16 parts
Calcium monohydrogen phosphate, 7.5~8.5 parts of corn protein powder, 4~5 parts of soybean oil, 1.2~1.8 parts of methionine, 0.8~1.2 part
Laying hen microelement, 0.18~0.22 part of laying hen vitamin, 0.3~0.5 part of choline chloride, 2.5~3.2 parts of reduction gallbladders
The additive of sterol forms.Wherein, the additive for reducing cholesterol is premixed by the fleece-flower root, hawthorn, Cassia occidentalis, bacillus subtilis
Agent and lactic acid bacteria pre-mixing agent composition, but the additive of above-mentioned reduction cholesterol is unknown for the effect for reducing egg cholesterol
It is aobvious.
Summary of the invention
The present invention in view of the above drawbacks of the prior art, provides a kind of feed addition that can reduce egg yolk cholesterol
Agent reduces the absorption of endogenous cholesterol, interferes the synthesis and metabolism of cholesterol, to guarantee the performance of reduction cholesterol effect.
In order to achieve the above objectives, the present invention adopts the following technical solutions:
A kind of feed addictive reducing egg yolk cholesterol, including following parts by weight component:
40-50 parts of alpha-linolenic acid
20-30 parts of conjugated linoleic acid
15-20 parts of green tea powder
10-15 parts of mulberry leaf powder
1-2 parts of curcumin
0.5-1 parts of stilbene grass extract
1-2 parts of Mao Xu Cao extract
0.05-0.1 parts of alfalfa saponin.
Preferably, the feed addictive for reducing egg yolk cholesterol is made of following parts by weight raw material:
40-50 parts of alpha-linolenic acid
20-30 parts of conjugated linoleic acid
15-20 parts of green tea powder
10-15 parts of mulberry leaf powder
1-2 parts of curcumin
0.5-1 parts of stilbene grass extract
1-2 parts of Mao Xu Cao extract
0.05-0.1 parts of alfalfa saponin.
It is further preferred that the feed addictive for reducing egg yolk cholesterol is by following parts by weight raw material group
At:
45 parts of alpha-linolenic acid
26 parts of conjugated linoleic acid
17 parts of green tea powder
12 parts of mulberry leaf powder
2 parts of curcumin
0.8 part of stilbene grass extract
1.6 parts of Mao Xu Cao extract
0.08 part of alfalfa saponin.
Each component and effect that the present invention can reduce the feed addictive of egg yolk cholesterol is as follows.
Alpha-linolenic acid (ALA):
Alpha-linolenic acid (ALA, 18 carbon, 9,12,15- trienic acid) is considered as a kind of more insatiable hungers necessary to many animals
With fatty acid (PUFA), ALA is by inhibiting to participate in fatty (acid) biosynthesis enzyme in liver so that the lipid for reducing laying hen closes
At and secretion, accelerate utilization of the peripheral tissues to lipid, finally reduce cholesterol level of vitellus.Poultry can convert EPA for ALA
And DHA, it is the parent of n-3 fatty acid, and the composition of PUFA and content will have a direct impact on the fatty acid composition of egg in feed
And content.Fat, the composition of fatty acid and the content of content, especially cholesterol and the saturation degree of fatty acid are to egg in feed
The content of cholesterol is affected.
Conjugated linoleic acid (Conjugated linoleic acids, CLA):
Animal experiment shows that CLA can regulate and control the dynamic equilibrium of body cholesterol, and when diet cholesterol is higher, CLA can subtract
The absorption of few cholesterol；Conversely, delaying the removing of cholesterol in blood plasma.Diet addition CLA significantly affects laying hen cholesterol metabolic
Relevant enzyme content, to reduce cholesterol level of vitellus.Test cell line proves that CLA can be activated in 3T3-L1 fat cell
AMPK reduces the content of triacylglycerol in fat cell, and CLA also can increase the activity of AMPK in animal body, therefore CLA can pass through
The activity of AMPK regulation HMGR is activated, and then influences the metabolism of laying hen cholesterol.
Green tea powder:
There are many new functions such as to reduce cardiovascular disease, inhibit tumour, drop for green tea powder (main component is tea polyphenols), tea
The effects of low cholesterolemia.The mechanism of tea polyphenols adjusting cholesterol are as follows: reduce the content of low-density lipoprotein in serum, improve
The content of high-density lipoprotein.In addition, tea polyphenols have very strong antioxygenic property, ALA in formula can be improved well
Utilizability and storage period with CLA.Artificial synthesized anti-oxidant such as traditional BHA, BHT, tert-butyl hydroquinone (TB-HQ)
Agent, although inoxidizability is strong, side effect is big, causes the increase of animal's liver, causes cholesterol in serum exceeded, has carcinogenic
Possibility, and the BHT of high dose will affect the lipid-metabolism of animal.Increasingly understanding with people to natural,
The concern of animal food safety is gradually reinforced, artificial synthesized antioxidant is supported because of its safety issue by consumer
System, tea polyphenols have caused people's most attention as a kind of novel antioxidant feed additive, the efficient characteristic of low toxicity.
Mulberry leaf powder:
Mulberry leaf powder is rich in high protein, and crude protein content contains crude fibre 8%~15%, soluble carbon water 15%~28%
Compound 20%~25%, dry matter basis crude protein 17%~30%, amino acid is complete, contain 17 kinds of amino acid, composition with
The amino acid composition of soybean is unanimous on the whole, and various amino acid account for the ratio trend of total amino acid unanimously.Vitamin content is high, rich
It is to guarantee important nutrient necessary to enzyme (such as AMPK) activity in animal body containing microelements of calcium, iron, zinc, selenium etc..Mulberry
Crude fibre in leaf can effectively fetter intestinal cholesterol, cholate, be allowed to excrete, to reduce cholesterol in egg
Content.Flavone compound in mulberry leaf has the function of anti-oxidant and reduces cholesterol, and antioxidation can be in certain journey
ALA and CLA is not oxidized in protection formula on degree.Furthermore also contain natural activity health care material such as chlorophyll, leaf in mulberry leaf powder
Green element equally has the function of reducing cholesterol.
Curcumin:
Curcumin has extensive antioxidant activity, can remove interior free yl, has liver protection and antitumaous effect, moreover it is possible to
The content for enough reducing internal cholesterol and triglycerides, plays a protective role to cardio-cerebrovascular.Curcumin can make test tube of hepari
The activity of the total lipolytic enzyme of blood plasma (PHTA), lipoprotein lipase (LPL) and hepatic lipase increases, to accelerate the generation of cholesterol lipoprotein
It thanks, significantly reduces serum and total cholesterol of liver content.Contain phenolic hydroxyl group in the molecular structure of curcumin, is turned into antioxygen
With the oxidation of ALA and CLA can be reduced to a certain extent.
Stilbene grass extract:
Stilbene grass extract is added in diet can improve the egg laying performance of laying hen, improve egg quality, can be by adjusting serum
Middle fat metabolism index and the expression reduction egg yolk gallbladder for inhibiting laying hen cholesterol metabolic relative enzyme gene (HMGR, VLDLR)
Sterol content.
Mao Xu Cao extract:
Mao Xu Cao is the important traditional herbal medicines of civil one kind in Perenniporia martius country, containing there are many chemical component, packets
Include flavonoids and terpene etc..In recent years, the pharmacological action of Mao Xu Cao is widely studied, the pharmacology that all pairs of Mao Xu Caos carry out
Research confirms it with biggish medical value.Mao Xu Cao has extremely strong antioxidant activity, can reduce to a certain extent
The oxidation of ALA and CLA.
Alfalfa saponin:
Saponins can generate compound not soluble in water in conjunction with cholesterol, prevent inside and outside source property cholesterol in intestines
Middle absorption, and can promote cholesterol degradation and be discharged at cholic acid, to reduce cholesterol level.
To sum up, feed addictive of the present invention is by reducing the absorption (alfalfa saponin, mulberry leaf powder) of endogenous cholesterol, influencing egg
The expression (stilbene grass extract) of chicken cholesterol metabolic relevant enzyme or gene and relevant enzyme activity (curcumin, mulberry leaf powder, CLA,
ALA), and then the synthesis and metabolism of cholesterol are interfered, (tea powder, mulberry leaf powder, curcumin, Mao Xu Cao extract in addition, part material
Object) there is very strong antioxidant activity, protection CLA, ALA is not oxidized, to guarantee the performance of reduction cholesterol effect, finally
Achieve the purpose that reduce egg yolk cholesterol level.
Preferably, the preparation method of the green tea powder includes the water with 10-15 times of green tea weight first to green tea 30
Tea is washed at a temperature of~50 DEG C, and then green tea is extracted with 20-25 times of green tea weight of water at a temperature of 80~90 DEG C,
The filtrate finally extracted is concentrated, dries the instant green tea powder that acquisition polyphenol content is greater than 80%.
Preferably, the preparation of the mulberry leaf powder includes being crushed mulberry leaf using pulverizer, and water and wind are added after crushing
Taste protease is digested；Finally extraction obtains solidliquid mixture, and filtration drying to obtain the final product.
Preferably, the stilbene grass extract is by Radix Astragali water boiling concentration extract, astragalus polyose and acetic acid in one's early teens in one's early teens
Ethyl ester extract is formed with weight ratio for the ratio mixing of 1-3:5-9:2-3.
Preferably, the Mao Xu Cao extract the preparation method comprises the following steps: by Mao Xu Cao using alcohol heat reflux method extract 3-5 it is small
When, obtained extracting solution carries out flash concentration using Reduced Pressure Concentration Device under the conditions of 60-70 DEG C, is dried in vacuo to obtain the final product.
Preferably, the alfalfa saponin the preparation method comprises the following steps: by the stem of smashed alfalfa and ethyl alcohol according to quality
Than being placed in microwave treater for the mixing of 1:2~3,20~30min is extracted under conditions of 40~60 DEG C, is taken after staticly settling
Supernatant liquor, it is 1.3~1.5g/mL that supernatant liquor, which is concentrated into density,.
The present invention also provides the application methods that described one kind can reduce the feed addictive of egg yolk cholesterol, including such as
Lower step:
S1. by alpha-linolenic acid, conjugated linoleic acid, green tea powder, mulberry leaf powder, curcumin, stilbene grass extract, Mao Xu Cao extract
And alfalfa saponin, feed addictive is obtained after mixing；By the feed addictive according to the weight of mass fraction 5%-10%
Amount is added in chicken feed, remixes uniformly, obtains feed A；
S2. it is continuously raised laying hen 40 days or more using feed A described in S1, laying hen is laying eggs for 150 ages in days or more
Chicken；
It S3. is low-cholesterol egg in laying period produced egg by the laying hen of S2 raising.
Preferably, feed addictive described in S1 is added in chicken feed according to the weight of mass fraction 8%-10%.
Compared with prior art, beneficial effects of the present invention:
The present invention is started with by the formula of the feed addictive that can reduce egg yolk cholesterol, in selection formula it is each at
Divide and constantly test adjusts the content proportion between each ingredient, can reach: by absorption (the clover soap of reduction endogenous cholesterol
Glycosides, mulberry leaf powder), influence laying hen cholesterol metabolic relevant enzyme or gene expression (stilbene grass extract) and relevant enzyme activity (ginger
Flavine, mulberry leaf powder, CLA, ALA), and then the synthesis and metabolism of cholesterol are interfered, in addition, material composition green tea powder, mulberry leaf powder, ginger
Flavine, Mao Xu Cao extract have very strong antioxidant activity, and protection CLA, ALA is not oxidized, to guarantee the hair of its effect
It waves, is finally reached the purpose for reducing egg yolk cholesterol level.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this
Invention be further described, but the present invention claims protection scope be not limited to embodiment.
Embodiment 1:
The application method of the feed addictive of egg yolk cholesterol can be reduced:
S1. by alpha-linolenic acid 40Kg, conjugated linoleic acid 20Kg, green tea powder 15Kg, mulberry leaf powder 10Kg, curcumin 1Kg, stilbene grass
Extract 0.5Kg, Mao Xu Cao extract 1Kg and alfalfa saponin 0.05Kg, obtain feed addictive after mixing；By the feeding
Feed additives are added in chicken feed according to the weight of mass fraction 6%, remix uniformly, obtain feed A；
S2. it is continuously raised laying hen 40 days or more using feed A described in S1, laying hen is laying eggs for 150 ages in days or more
Chicken；
It S3. is low-cholesterol egg in laying period produced egg by the laying hen of S2 raising.
Embodiment 2:
The application method of the feed addictive of egg yolk cholesterol can be reduced:
S1. by alpha-linolenic acid 50Kg, conjugated linoleic acid 30Kg, green tea powder 20Kg, mulberry leaf powder 15Kg, curcumin 2Kg, stilbene grass
Extract 1Kg, Mao Xu Cao extract 2Kg and alfalfa saponin 0.1Kg, obtain feed addictive after mixing；By the feed
Additive is added in chicken feed according to the weight of mass fraction 10%, remixes uniformly, obtains feed A；
S2. it is continuously raised laying hen 40 days or more using feed A described in S1, laying hen is laying eggs for 150 ages in days or more
Chicken；
It S3. is low-cholesterol egg in laying period produced egg by the laying hen of S2 raising.
Embodiment 3:
The application method of the feed addictive of egg yolk cholesterol can be reduced:
S1. by alpha-linolenic acid 45Kg, conjugated linoleic acid 26Kg, green tea powder 17Kg, mulberry leaf powder 12Kg, curcumin 2Kg, stilbene grass
Extract 0.8Kg, Mao Xu Cao extract 1.6Kg and alfalfa saponin 0.08Kg, obtain feed addictive after mixing；It will be described
Feed addictive is added in chicken feed according to the weight of mass fraction 8%, remixes uniformly, obtains feed A；
S2. it is continuously raised laying hen 40 days or more using feed A described in S1, laying hen is laying eggs for 150 ages in days or more
Chicken；
It S3. is low-cholesterol egg in laying period produced egg by the laying hen of S2 raising.
Embodiment 4:
The application method of the feed addictive of egg yolk cholesterol can be reduced:
S1. by alpha-linolenic acid 42Kg, conjugated linoleic acid 24Kg, green tea powder 17Kg, mulberry leaf powder 13Kg, curcumin 1.4Kg, stilbene
Careless extract 0.7Kg, Mao Xu Cao extract 1.3Kg and alfalfa saponin 0.09Kg, obtain feed addictive after mixing；By institute
It states feed addictive to be added in chicken feed according to the weight of mass fraction 7%, remixes uniformly, obtain feed A；
S2. it is continuously raised laying hen 40 days or more using feed A described in S1, laying hen is laying eggs for 150 ages in days or more
Chicken；
It S3. is low-cholesterol egg in laying period produced egg by the laying hen of S2 raising.
Comparative example 1:
Compared with Example 1, Mao Xu Cao extract is not contained, other operations are same as Example 1.
Comparative example 2:
Compared with Example 1, stilbene grass extract is not contained, other operations are same as Example 1.
Comparative example 3:
Compared with Example 1, alfalfa saponin, mulberry leaf powder are not contained, other operations are same as Example 1.
Comparative example 4:
Compared with Example 1, each component content is different, alpha-linolenic acid 36Kg, conjugated linoleic acid 15Kg, green tea powder 15Kg,
Mulberry leaf powder 10Kg, curcumin 1Kg, stilbene grass extract 0.5Kg, Mao Xu Cao extract 1Kg and alfalfa saponin 0.05Kg.Other operations
It is same as Example 1.
Comparative example 5:
Compared with Example 1, feed addictive is added without in the chicken feed, other operations are same as Example 1.
Performance test
Its cholesterol level is tested to embodiment 1-4 and comparative example the 1-5 egg produced, thus judge embodiment 1-4 and
Influence of the feed addictive of comparative example 1-4 to yolk cholesterol concentration, test result such as table 1.
Test method are as follows: this experiment is measured using CHOD-PAP method, and kit is purchased from the limited public affairs of Shanghai Rong Sheng biotechnology
Department.
Influence of 1 feed addictive of table to yolk cholesterol concentration
| Processing group | Cholesterol (mg/g) | Improve percentage |
| Comparative example 5 | 13.40±0.53a | - |
| Embodiment 1 | 12.35±0.33b | 7.84% |
| Embodiment 2 | 12.15±0.43b | 9.33% |
| Embodiment 3 | 11.80±0.43b | 11.94% |
| Embodiment 4 | 12.55±0.56b | 6.34% |
| Comparative example 1 | 13.33±0.36 | - |
| Comparative example 2 | 12.90±0.46 | - |
| Comparative example 3 | 12.70±0.56 | - |
| Comparative example 4 | 13.56±0.68 | - |
Note: digital shoulder marking-up is female in table, and vertical setting of types difference indicates significant difference (P < 0.05).
As shown in table 1, feed addictive of the invention is added in the feed of lower laying hen, to the gallbladder of the egg of final production
Sterol concentration has significant reducing effect.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and is modified.Therefore, the invention is not limited to the specific embodiments disclosed and described above, to the one of invention
A little modifications and changes should also be as falling into the scope of the claims of the present invention.In addition, although being used in this specification
Some specific terms, these terms are merely for convenience of description, does not limit the present invention in any way.
Claims (10)
1. the feed addictive that one kind can reduce egg yolk cholesterol, which is characterized in that including following parts by weight component:
40-50 parts of alpha-linolenic acid
20-30 parts of conjugated linoleic acid
15-20 parts of green tea powder
10-15 parts of mulberry leaf powder
1-2 parts of curcumin
0.5-1 parts of stilbene grass extract
1-2 parts of Mao Xu Cao extract
0.05-0.1 parts of alfalfa saponin.
2. a kind of feed addictive that can reduce egg yolk cholesterol according to claim 1, which is characterized in that by as follows
Parts by weight raw material composition:
40-50 parts of alpha-linolenic acid
20-30 parts of conjugated linoleic acid
15-20 parts of green tea powder
10-15 parts of mulberry leaf powder
1-2 parts of curcumin
0.5-1 parts of stilbene grass extract
1-2 parts of Mao Xu Cao extract
0.05-0.1 parts of alfalfa saponin.
3. the feed addictive that one kind according to claim 1 or claim 2 can reduce egg yolk cholesterol, which is characterized in that by
Following parts by weight raw material composition:
45 parts of alpha-linolenic acid
26 parts of conjugated linoleic acid
17 parts of green tea powder
12 parts of mulberry leaf powder
2 parts of curcumin
0.8 part of stilbene grass extract
1.6 parts of Mao Xu Cao extract
0.08 part of alfalfa saponin.
4. a kind of feed addictive that can reduce egg yolk cholesterol according to claim 1, which is characterized in that described green
The preparation method of tea powder includes first washing tea at a temperature of 30~50 DEG C to green tea with 10-15 times of green tea weight of water, then
Green tea is extracted with 20-25 times of green tea weight of water at a temperature of 80~90 DEG C, the filtrate finally extracted carries out dense
Contracting, the dry instant green tea powder for obtaining polyphenol content and being greater than 80%.
5. a kind of feed addictive that can reduce egg yolk cholesterol according to claim 1, which is characterized in that the mulberry
The preparation of leaf powder includes being crushed mulberry leaf using pulverizer, and water is added after crushing and flavor protease is digested；Finally
Extraction obtains solidliquid mixture, and filtration drying to obtain the final product.
6. a kind of feed addictive that can reduce egg yolk cholesterol according to claim 1, which is characterized in that the stilbene
Careless extract is by Radix Astragali water boiling concentration extract, astragalus polyose and in one's early teens ethyl acetate extract with weight ratio is 1- in one's early teens
The ratio of 3:5-9:2-3 mixes composition.
7. a kind of feed addictive that can reduce egg yolk cholesterol according to claim 1, which is characterized in that the cat
Moss extract the preparation method comprises the following steps: by Mao Xu Cao using alcohol heat reflux method extract 3-5 hours, obtained extracting solution is in 60-
Flash concentration is carried out using Reduced Pressure Concentration Device under the conditions of 70 DEG C, is dried in vacuo to obtain the final product.
8. a kind of feed addictive that can reduce egg yolk cholesterol according to claim 1, which is characterized in that the lucerne
Mu saponin the preparation method comprises the following steps: by the stem of smashed alfalfa and ethyl alcohol according to mass ratio be 1:2~3 mix be placed on it is micro-
In wave processor, 20~30min is extracted under conditions of 40~60 DEG C, takes supernatant liquor after staticly settling, supernatant liquor is dense
Being reduced to density is 1.3~1.5g/mL.
9. any described one kind of claim 1-8 can reduce the application method of the feed addictive of egg yolk cholesterol, special
Sign is, includes the following steps:
S1. by alpha-linolenic acid, conjugated linoleic acid, green tea powder, mulberry leaf powder, curcumin, stilbene grass extract, Mao Xu Cao extract and lucerne
Mu saponin, obtains feed addictive after mixing；The feed addictive is added according to the weight of mass fraction 5%-10%
It is added in chicken feed, remixes uniformly, obtain feed A；
S2. it is continuously raised laying hen 40 days or more using feed A described in S1, laying hen is the laying hen of 150 ages in days or more；
It S3. is low-cholesterol egg in laying period produced egg by the laying hen of S2 raising.
10. a kind of application method for the feed addictive that can reduce egg yolk cholesterol according to claim 9, feature
It is, feed addictive described in S1 is added in chicken feed according to the weight of mass fraction 8%-10%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110463856A (en) * | 2019-09-05 | 2019-11-19 | 广东驱动力生物科技股份有限公司 | A kind of feed addictive improving Layer Production Performance |
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