CN110133129A - The detection method of mannitol in a kind of fermentation cordyceps (Cs-4) - Google Patents
The detection method of mannitol in a kind of fermentation cordyceps (Cs-4) Download PDFInfo
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- CN110133129A CN110133129A CN201910400503.0A CN201910400503A CN110133129A CN 110133129 A CN110133129 A CN 110133129A CN 201910400503 A CN201910400503 A CN 201910400503A CN 110133129 A CN110133129 A CN 110133129A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
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- G01N2030/146—Preparation by elimination of some components using membranes
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Abstract
The present invention relates to a kind of detection methods of mannitol in fermentation cordyceps (Cs-4), the method comprises the following steps: step 1, the preparation of test solution: take fermentation cordyceps (Cs-4) appropriate, it is accurately weighed, ethanol solution is added, weighed weight, is heated to reflux, and lets cool, weighed weight again, the weight of less loss is supplied with ethanol solution, is filtered, is taken subsequent filtrate in right amount into evaporating dish, it is dried under reduced pressure to close dry, residue is dissolved in volumetric flask with mobile phase, is crossed 0.45 μm of miillpore filter, is taken subsequent filtrate as test solution；Step 2, the preparation of reference substance solution: taking PEARLITOL 25C reference substance appropriate, accurately weighed, adds flowing phased soln, obtains reference substance solution；Step 3, it measures: it is accurate respectively to draw reference substance solution and test solution, liquid chromatograph is injected, measurement obtains chromatogram, and the content of PEARLITOL 25C (C6H14O6) is calculated according to chromatogram.
Description
Technical field:
The present invention relates to a kind of detection method of drug ingedient, in particular to sweet dew in a kind of fermentation cordyceps (Cs-4)
The content assaying method of alcohol.
Background technique:
Fermentation cordyceps are section ergot fungus cordyceps sinensis isolated from the fresh cordyceps sinensis in Qinghai
The asexual generation of [Cordyceps sinensis (Berk.) Sae.] -- Chinese synnema spore (Synnematum sinense
Yin et Shen sp.now) strain is through mycelial dried powder obtained by liquid fermentation and culture.In fermentation cordyceps, one
The particularly effective bacterial strain (Cs-4) of kind can obtain fermentation cordyceps (Cs-4) by fermented and cultured, by clinical verification, hair
There is ferment cordyceps (Cs-4) kidney tonifying to protect lung, secret lean airway dysfunction.For chronic bronchitis, hyperlipemia, impotence, something lost
The sexual disorders such as essence, premature ejaculation, sexual hypoesthesia, menoxenia, soreness of waist abdominal pain, clear and thin leucorrhea, soreness and weakness of waist and knees, chilly limb
The old age symptoms such as the chronic diseases marked by deficiency of vital energy and advanced stage blood vampire disease cirrhosis such as cold, dizzy, tinnitus.
PEARLITOL 25C belongs to the substance English name of sugar alcohol one kind: D-Mannitol, is called the close alcohol of D-mannitol D- sweet dew；
D- C6H14O6；D-Mannitol；D-mannital；Cordycepic acid；Piperazine is muttered mannose etc..The colourless of sweet taste is slightly put similar to sucrose
Or white crystalline powder.PEARLITOL 25C is considered as the primary bioactive components of fermentation cordyceps and its product kind, is to comment
Therefore the important indicator of valence fermentation cordyceps and its product property records the Jinshuibao piece into Chinese Pharmacopoeia, capsule and hundred enables
Capsule, and record zhiling capsules, Ningxinbao Capsules and Xinganbao Jrao nang into the Sanitation Ministry medicine standard, with mannitol be containing
Measure fixed index.
At present to mannitol detection technique in cordyceps sinensis and its product have volumetric analysis, thin-layered chromatography, HPLC-ELSD,
HPLC-RID, Secondary Ion Mass Spectrometry etc..The document report having disclosed artificial culture cordyceps sinensis PEARLITOL 25C containing measuring
It is fixed -- " biology magazine " 03 phase in 2012, HPLC-ELSD method measure the content of mannitol in Yupingfeng Granules, PEARLITOL 25C inspection
Test agent box, gas chromatography measure content -- " Changchun Traditional Chinese Medical College's journal ", the ultraviolet spectrometry light of PEARLITOL 25C in Cordyceps militaris
Degree method measures the content-" study of pharmacy " 2006 of PEARLITOL 25C in cordyceps sinensis.
Since fermentation cordyceps of the invention are fermentation cordyceps (Cs-4), contain the spy different from other cordyceps sinensis
Different ingredient, thus the method that wherein measurement of mannitol content is not available the prior art, by the present inventor's multiple authentication,
Existing method is not suitable for the present invention.
For this purpose, the present inventor, using high performance liquid chromatography (HPLC-RID), obtains a kind of accuracy by screening
Height, easy to operate, precision, stability, the good completely new detection method of repeatability.
Summary of the invention:
The purpose of the present invention is to provide a kind of detection methods of new fermentation cordyceps (Cs-4).For this purpose, of the invention
A kind of method measuring mannitol content in fermentation cordyceps (Cs-4) is provided.
The present invention is realized by following technology:
The present invention provides a kind of detection method of mannitol in fermentation cordyceps (Cs-4), which is characterized in that step is such as
Under:
Step 1, the preparation of test solution:
Take fermentation cordyceps (Cs-4) in right amount, it is accurately weighed, ethanol solution is added, weighed weight is heated to reflux, puts
It is cold, then weighed weight, the weight of less loss is supplied with ethanol solution, filters, takes subsequent filtrate in right amount into evaporating dish, be dried under reduced pressure to
Close dry, residue is dissolved in volumetric flask with mobile phase, is crossed 0.45 μm of miillpore filter, is taken subsequent filtrate as test solution；
Step 2, the preparation of reference substance solution:
Take PEARLITOL 25C reference substance appropriate, it is accurately weighed, add flowing phased soln, obtains reference substance solution；
Step 3, it measures:
It is accurate respectively to draw reference substance solution and test solution, liquid chromatograph is injected, measurement obtains chromatogram, according to
Chromatogram calculates the content of PEARLITOL 25C (C6H14O6)；
Wherein chromatographic condition is as follows: chromatographic column Agilent Zorbax NH2, and for mobile phase with acetonitrile for A phase, water is B phase,
Carry out gradient elution.
Detection method of the invention, preferred steps are as follows:
Step 1, the preparation of test solution
Fermentation cordyceps (Cs-4) 1-4g is taken, it is accurately weighed, the ethanol solution of 25-100ml 50-100% is added, claims
Determine weight, be heated to reflux 60-120min, let cool, then weighed weight, the weight of less loss is supplied with the ethyl alcohol alcoholic solution of 50-100%
Amount, filtration, takes subsequent filtrate 10-50ml into evaporating dish, is dried under reduced pressure to close and does, and residue flowing phased soln is simultaneously settled to 5-
In 25ml volumetric flask, takes in right amount, cross 0.45 μm of miillpore filter, take subsequent filtrate as test solution；
Step 2, the preparation of reference substance solution:
Take PEARLITOL 25C reference substance appropriate, it is accurately weighed, add flowing phased soln to be made every 1ml 1-5mg's containing PEARLITOL 25C
Reference substance solution；
Step 3, it measures:
It is accurate respectively to draw reference substance solution and test solution, liquid chromatograph is injected, measurement obtains chromatogram, according to
Chromatogram calculates the content of PEARLITOL 25C (C6H14O6)；
Wherein chromatographic condition is as follows: chromatographic column Agilent Zorbax NH2, column length 15-25cm, internal diameter 2-
8mm, partial size are 2-10 μm；With acetonitrile-water (70~90:30~10, v:v) for mobile phase, flow velocity 0.5-1.0ml/min；Into
Sample amount is 5-50 μ l；Column temperature is 20-40 DEG C；20-40 DEG C of differential refraction detector temperature；Number of theoretical plate should not by the calculating of adenosine peak
Lower than 2000-5000.
Detection method of the invention, most preferred steps are as follows:
Step 1, the preparation of test solution:
Fermentation cordyceps (Cs-4) 4g is taken, it is accurately weighed, ethanol solution 50ml is added, weighed weight is heated to reflux 2h,
It lets cool, then weighed weight, the weight of less loss is supplied with ethanol solution, filter, take subsequent filtrate 20ml into evaporating dish, be dried under reduced pressure
It is done to close, residue adds flowing phased soln and is settled in 10ml volumetric flask, takes in right amount, crosses 0.45 μm of miillpore filter, filtrate is taken to make
For test solution；
Step 2, the preparation of reference substance solution:
Take PEARLITOL 25C reference substance appropriate, precision weighs, and adds mobile phase that reference substance solution of every 1ml containing 4mg is made, i.e.,
?；
Step 3, it measures:
It is accurate respectively to draw 20 μ l of reference substance solution and 20 μ l of test solution, it is injected separately into liquid chromatograph, is measured
To chromatogram, the content of PEARLITOL 25C (C6H14O6) is calculated according to chromatogram.
Most preferably, wherein chromatographic condition is as follows: shining Pharmacopoeia of People's Republic of China VI D efficient liquid phase of version annex in 2015
Chromatography experiment, chromatographic column are Agilent Zorbax NH2, column length 250mm, internal diameter 4.6mm, and partial size is 5 μm；Stream
Dynamic phase is with acetonitrile-water (78:22, v:v)；Flow velocity is 1.0ml/min；Column temperature is 40 DEG C；Differential refraction detector temperature is 35 DEG C；
Number of theoretical plate is calculated by adenosine peak should be not less than 3000.
Wherein, PEARLITOL 25C reference substance is existing product, can be bought on the market.
Wherein, subsequent filtrate refers to: the filtrate being first filtered is known as primary filtrate, and filtrate degree of purity at this time is inadequate, abandons
It goes, continues collected filtrate after primary filtrate is outwelled, be exactly subsequent filtrate.
Measuring method of the invention is obtained by screening, and screening process is as follows:
1. the selection of extraction conditions
The selection of 1.1 extracting methods
The present invention extracts mannitol in fermentation cordyceps (Cs-4) using reflux extraction and ultrasonic extraction.Pass through ratio
Compared with discovery, the extraction efficiency of ultrasonic extraction is far below reflux extraction, can not be complete in advance to mannitol in sample, therefore selects
Use reflux extraction.
The selection of 1.2 Extraction solvents
Investigate the extraction effect of ethyl alcohol, methanol as Extraction solvent when, the results showed that, the extraction effect of ethyl alcohol is higher.
The extraction effect of the ethyl alcohol of various concentration is compared again, and when finding 100% ethanol solution as Extraction solvent, extraction effect is most
It is good.
The selection of 1.3 extraction times
1h, 1.5h, 2h and 2.5h heating extraction time are investigated respectively, the results showed that, the heating extraction time is to mentioning
Fine distinction is only presented after taking the influence 2h of efficiency, therefore selects 2h as the ultrasonic extraction time.
2. the optimization of chromatographic condition
The selection of 2.1 mobile phases
For mobile phase with acetonitrile for A phase, water is B phase, is examined respectively A-B (78:22, V:V) and A-B (85:15, v:v)
It examines, the results showed that when having preferable separating degree between the mobile phase main peak and impurity peaks of two ratios and A-B (78:22, V:V) appearance
Between it is shorter, shorten analysis time.
2.2 differential pulse polarograpll temperature and column temperature selection
There is a Duan Guanlu in view of column oven flows into detector, therefore sets the setting of column oven temperature and compare differential refraction detector
Temperature is 5 DEG C high, investigates 35 DEG C of column oven, 30 DEG C of differential refraction detector；40 DEG C of column oven, 35 DEG C of differential refraction detector.Knot
Fruit shows 40 DEG C of column oven, and 35 DEG C of reproducibility of differential refraction detector are preferable.
Detection method of the invention controls more effectively the quality of product, and accuracy, sensitivity, stability are higher.
Method of the invention not only can be used for the quality control of mannitol in fermentation cordyceps (Cs-4), but also can be used for worm of fermenting
Grass volvatus powder (Cs-4) is the quality control of mannitol in other preparations of raw material, such as tablet, granule, honeyed bolus, oral solution etc..
Beneficial effects of the present invention are further illustrated below by way of data form:
Detailed description of the invention:
The chemical structure of Fig. 1 mannitol；
The liquid phase of the liquid chromatogram (a) of Fig. 2 solvent blank, the liquid chromatogram (b) of reference substance solution, test solution
Chromatogram (c).
Specific embodiment:
The present invention is further illustrated by the following examples, but not as limitation of the present invention.
Embodiment 1
This product 2g is taken, it is accurately weighed, ethanol solution 25ml is added, weighed weight is heated to reflux 2h, lets cool, then weighed heavy
Amount, the weight of less loss is supplied with ethanol solution, is filtered, is taken subsequent filtrate 10ml into evaporating dish, is dried under reduced pressure to close and is done, residue adds
Flowing phased soln is simultaneously settled in 10ml volumetric flask, is taken in right amount, is crossed 0.45 μm of miillpore filter, take filtrate as test solution.
The preparation of reference substance solution: taking PEARLITOL 25C reference substance appropriate, and precision weighs, and adds mobile phase to be dissolved into every 1ml and contains
Have PEARLITOL 25C 2mg to get.
Chromatographic condition is as follows: according to Pharmacopoeia of People's Republic of China VI D high performance liquid chromatography of version annex test in 2015, color
Spectrum column is Agilent Zorbax NH2, column length 250mm, internal diameter 4.6mm, and partial size is 5 μm；Mobile phase is with acetonitrile-water
(78:22, v:v)；Flow velocity is 1.0ml/min；Column temperature is 40 DEG C；Differential refraction detector temperature is 35 DEG C
Measuring method: it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, it is injected separately into liquid chromatograph, is surveyed
Surely chromatogram is obtained, the content of PEARLITOL 25C (C6H14O6) is calculated according to chromatogram.
In this method carry out system suitability test, since the concentration of PEARLITOL 25C in reference substance solution is lower, cause be
Reproducibility is poor in applicability of uniting, and main peak area is smaller in test solution, and the deviation of parallel determination is larger.
Embodiment 2
This product 4g is taken, it is accurately weighed, ethanol solution 50ml is added, weighed weight is heated to reflux 2h, lets cool, then weighed heavy
Amount, the weight of less loss is supplied with ethanol solution, is filtered, is taken subsequent filtrate 20ml into evaporating dish, is dried under reduced pressure to close and is done, residue adds
Flowing phased soln is simultaneously settled in 10ml volumetric flask, is taken in right amount, is crossed 0.45 μm of miillpore filter, take filtrate as test solution.
The preparation of reference substance solution: taking PEARLITOL 25C reference substance appropriate, and precision weighs, and adds mobile phase to be dissolved into every 1ml and contains
Have PEARLITOL 25C 4mg to get.
Chromatographic condition is as follows: according to Pharmacopoeia of People's Republic of China VI D high performance liquid chromatography of version annex test in 2015, color
Spectrum column is Agilent Zorbax NH2, column length 250mm, internal diameter 4.6mm, and partial size is 5 μm；Mobile phase is with acetonitrile-water
(78:22, v:v)；Flow velocity is 1.0ml/min；Column temperature is 40 DEG C；Differential refraction detector temperature is 35 DEG C
Measuring method: it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, it is injected separately into liquid chromatograph, is surveyed
Surely chromatogram is obtained, the content of PEARLITOL 25C (C6H14O6) is calculated according to chromatogram.
Reference substance solution and the concentration of test solution are increased in this method, measurement result indicates that system is applicable in
Property it is good, reproducibility has greatly improved, and the deviation that test solution is measured in parallel reduces, but the peak shape of main peak is dragged there are micro-
Tail, it is to be improved.
Embodiment 3
This product 4g is taken, it is accurately weighed, ethanol solution 50ml is added, weighed weight is heated to reflux 2h, lets cool, then weighed heavy
Amount, the weight of less loss is supplied with ethanol solution, is filtered, is taken subsequent filtrate 20ml into evaporating dish, is dried under reduced pressure to close and is done, residue adds
Flowing phased soln is simultaneously settled in 10ml volumetric flask, is taken in right amount, is crossed 0.45 μm of miillpore filter, take filtrate as test solution.
Chromatographic condition is as follows: according to Pharmacopoeia of People's Republic of China VI D high performance liquid chromatography of version annex test in 2015, color
Spectrum column is Agilent Zorbax NH2, column length 250mm, internal diameter 4.6mm, and partial size is 5 μm；Mobile phase is with acetonitrile-water
(85:15, v:v)；Flow velocity is 1.0ml/min；Column temperature is 40 DEG C；Differential refraction detector temperature is 35 DEG C
Measuring method: precision draws 20 μ l of test solution, is injected separately into liquid chromatograph, measures.
Mobile phase ratio is modified in this method, it is intended to improve peak shape.The result shows that increase the ratio of organic phase in mobile phase,
Script main peak retention time about 15min is set to increase to 24min, but peak shape has no improvement, and the mobile phase of two kinds of ratios
Middle main peak and impurity peaks have preferable separating degree.
Embodiment 4
This product 4g is taken, it is accurately weighed, ethanol solution 50ml, weighed weight is added, ultrasonic 30min is let cool, then weighed heavy
Amount, the weight of less loss is supplied with ethanol solution, is filtered, is taken subsequent filtrate 20ml into evaporating dish, is dried under reduced pressure to close and is done, residue adds
Flowing phased soln is simultaneously settled in 10ml volumetric flask, is taken in right amount, is crossed 0.45 μm of miillpore filter, take filtrate as test solution.
Chromatographic condition is as follows: according to Pharmacopoeia of People's Republic of China VI D high performance liquid chromatography of version annex test in 2015, color
Spectrum column is Agilent Zorbax NH2, column length 250mm, internal diameter 4.6mm, and partial size is 5 μm；Mobile phase is with acetonitrile-water
(78:22, v:v)；Flow velocity is 1.0ml/min；Column temperature is 40 DEG C；Differential refraction detector temperature is 35 DEG C
Measuring method: precision draws 20 μ l of test solution, is injected separately into liquid chromatograph, measures.
Ultrasonic extraction is selected in this method.The peak height of mannitol and peak area, which are much smaller than, in measurement result is heated to reflux
The peak height and peak area of mannitol in extraction, showing can not be completely complete by sweet dew alcohol extracting in sample in ultrasonic extraction.
From the results, it was seen that the HPLC of index components mannitol is surveyed in the fermentation cordyceps (Cs-4) that this experiment is established
It is easy, accurate that the method for determining has the advantages that, therefore is suitable as index components for fermentation cordyceps (Cs-4) prepared slices of Chinese crude drugs
Quality control.
Claims (4)
1. the detection method of mannitol in a kind of fermentation cordyceps (Cs-4), which is characterized in that steps are as follows:
Step 1, the preparation of test solution:
Take fermentation cordyceps (Cs-4) in right amount, it is accurately weighed, ethanol solution is added, weighed weight is heated to reflux, lets cool, then
Weighed weight supplies the weight of less loss with ethanol solution, and filtration takes subsequent filtrate in right amount into evaporating dish, is dried under reduced pressure to close and does,
Residue is dissolved in volumetric flask with mobile phase, is crossed 0.45 μm of miillpore filter, is taken subsequent filtrate as test solution；
Step 2, the preparation of reference substance solution:
Take PEARLITOL 25C reference substance appropriate, it is accurately weighed, add flowing phased soln, obtains reference substance solution；
Step 3, it measures:
It is accurate respectively to draw reference substance solution and test solution, liquid chromatograph is injected, measurement obtains chromatogram, according to chromatography
Figure calculates the content of PEARLITOL 25C (C6H14O6)；
Wherein chromatographic condition is as follows: chromatographic column Agilent Zorbax NH2, and for mobile phase with acetonitrile for A phase, water is B phase, carries out
Gradient elution.
2. detection method according to claim 1, which is characterized in that steps are as follows:
Step 1, the preparation of test solution
Fermentation cordyceps (Cs-4) 1-4g is taken, it is accurately weighed, the ethanol solution of 25-100ml 50-100% is added, it is weighed heavy
Amount, is heated to reflux 60-120min, lets cool, then weighed weight, the weight of less loss is supplied with the ethyl alcohol alcoholic solution of 50-100%, filters
It crosses, takes subsequent filtrate 10-50ml into evaporating dish, be dried under reduced pressure to close and do, residue flowing phased soln is simultaneously settled to 5-25ml appearance
In measuring bottle, takes in right amount, cross 0.45 μm of miillpore filter, take subsequent filtrate as test solution；
Step 2, the preparation of reference substance solution:
Take PEARLITOL 25C reference substance appropriate, it is accurately weighed, add flowing phased soln that the control of every 1ml 1-5mg containing PEARLITOL 25C is made
Product solution；
Step 3, it measures:
It is accurate respectively to draw reference substance solution and test solution, liquid chromatograph is injected, measurement obtains chromatogram, according to chromatography
Figure calculates the content of PEARLITOL 25C (C6H14O6)；
Wherein chromatographic condition is as follows: chromatographic column Agilent Zorbax NH2, column length 15-25cm, internal diameter 2-8mm, grain
Diameter is 2-10 μm；With acetonitrile-water (70~90:30~10, v:v) for mobile phase, flow velocity 0.5-1.0ml/min；Sample volume is
5-50μl；Column temperature is 20-40 DEG C；20-40 DEG C of differential refraction detector temperature；Number of theoretical plate should be not less than by the calculating of adenosine peak
2000-5000。
3. detection method according to claim 1, which is characterized in that steps are as follows:
Step 1, the preparation of test solution:
Fermentation cordyceps (Cs-4) 4g is taken, it is accurately weighed, ethanol solution 50ml is added, weighed weight is heated to reflux 2h, puts
It is cold, then weighed weight, the weight of less loss is supplied with ethanol solution, filters, takes subsequent filtrate 20ml into evaporating dish, be dried under reduced pressure to
Close dry, residue adds flowing phased soln and is settled in 10ml volumetric flask, takes in right amount, crosses 0.45 μm of miillpore filter, take filtrate conduct
Test solution；
Step 2, the preparation of reference substance solution:
Take PEARLITOL 25C reference substance appropriate, precision weighs, add mobile phase be made reference substance solution of every 1ml containing 4mg to get；
Step 3, it measures:
It is accurate respectively to draw 20 μ l of reference substance solution and 20 μ l of test solution, it is injected separately into liquid chromatograph, measurement obtains color
Spectrogram calculates the content of PEARLITOL 25C (C6H14O6) according to chromatogram.
4. detection method according to claim 1, which is characterized in that wherein chromatographic condition is as follows: according to Chinese people's republicanism
VI D high performance liquid chromatography of the version annex test in 2015 of state's pharmacopeia, chromatographic column is Agilent Zorbax NH2, and column length is
250mm, internal diameter 4.6mm, partial size are 5 μm；Mobile phase is with acetonitrile-water (78:22, v:v)；Flow velocity is 1.0ml/min；Column temperature is
40℃；Differential refraction detector temperature is 35 DEG C；Number of theoretical plate is calculated by adenosine peak should be not less than 3000.
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Cited By (2)
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|---|---|---|---|---|
| CN115078596A (en) * | 2022-08-18 | 2022-09-20 | 江西国药有限责任公司 | Method for detecting genistein in fermented cordyceps sinensis powder Cs-4 |
| CN115728412A (en) * | 2022-11-09 | 2023-03-03 | 广东汉潮中药科技有限公司 | Method for detecting content of cordycepic acid in fermented cordyceps sinensis powder (Cs-4) |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078596A (en) * | 2022-08-18 | 2022-09-20 | 江西国药有限责任公司 | Method for detecting genistein in fermented cordyceps sinensis powder Cs-4 |
| CN115728412A (en) * | 2022-11-09 | 2023-03-03 | 广东汉潮中药科技有限公司 | Method for detecting content of cordycepic acid in fermented cordyceps sinensis powder (Cs-4) |
| CN115728412B (en) * | 2022-11-09 | 2023-11-03 | 广东汉潮中药科技有限公司 | Method for detecting cordycepic acid content in fermented cordyceps sinensis powder (Cs-4) |
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