CN110133062A - A kind of detection method of evening primrose oil oxidation stability - Google Patents
A kind of detection method of evening primrose oil oxidation stability Download PDFInfo
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- CN110133062A CN110133062A CN201910489894.8A CN201910489894A CN110133062A CN 110133062 A CN110133062 A CN 110133062A CN 201910489894 A CN201910489894 A CN 201910489894A CN 110133062 A CN110133062 A CN 110133062A
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Abstract
The present invention provides a kind of detection methods of evening primrose oil oxidation stability, the following steps are included: a) carrying out quantitative detection to the dielectric constant of the evening primrose oil of different degree of oxidation respectively using the quantitative detecting method for the evening primrose oil dielectric constant established, the dielectric constant of evening primrose oil and the relationship of degree of oxidation are obtained；B) the dielectric constant progress quantitative detection for surveying oenothera biennis oil samples is treated, testing result is compared with the dielectric constant of the obtained evening primrose oil of step a) with the relationship of degree of oxidation, obtains the testing result of evening primrose oil oxidation stability.The present invention proposes a kind of detection method of evening primrose oil oxidation stability on the basis of the data relationship to a large amount of peroxide value of evening primrose oil and dielectric constant is analyzed, and detects its oxidation stability by measuring the dielectric constant of evening primrose oil.Detection method accuracy provided by the invention is good and easy to operate, plays an important role to further the improving for Quality Detection standard of evening primrose oil.
Description
Technical field
The present invention relates to vegetable fat Quality Detection technical fields, are to be related to a kind of evening primrose oil oxidation more specifically
The detection method of stability.
Background technique
Edible oil and fat are human diet important components, are one of mankind's three nutritious elements, and main ingredient is various
The glyceride of higher fatty acids, fatty acid are usually the straight-chain carboxylic acid containing even-numbered carbon atom, are divided into saturated fatty acid (such as tristearin
Beans bandit acid, lauric acid, capric acid, octanoic acid and the caproic acids such as acid, palmitic acid, beans bandit acid, lauric acid, capric acid, octanoic acid and caproic acid etc.) and not
Saturated fatty acid (such as oleic acid, linoleic acid, linolenic acid etc.)；In vegetable fat, Unsaturatcd fatty acid glycerides content is higher, fusing point
It is lower, it is in a liquid state at room temperature, while the type of fatty acid contained by the vegetable fat of different cultivars and ratio are different.
Grease by factors such as air, light, temperature during storage due to being influenced, Yi Fasheng autoxidation, to produce
Raw various peroxide.Although colorless and odorless of peroxide caused by the oxidation of grease itself is nontoxic, to the shadow of oil quality
It rings little.But peroxide is very unstable, is easily decomposed into many secondary oxidation products, such as aldehydes, ketone, alcohols, wherein
Some compounds certain harm can be generated to human body.Therefore the peroxide value of measurement grease is necessary.Peroxide value table
Show the quantity for the hydroperoxides that grease autoxidation initial stage is formed, value is higher, and the degree for showing that fatty acid is aoxidized is cured
By force；It when peroxide value starts apparent increase, shows grease and is largely oxidized, the stability of grease reduces, and fatty acid occurs
It is rancid.Generated peroxide also will continue to be oxidized and decompose and cause the rancid rotten of grease after Oxidation of Fat and Oils.Due to oil
Peroxide after lipid oxidation is sufficiently complex, and it is also highly difficult directly to measure its concrete content, therefore measurement grease peroxide at present
The method of compound is all to measure its indirect content mostly.
Traditional Oxidation of Fat and Oils analysis method is mostly chemical method, i.e., by chemical means measure peroxide value, anisidine value,
Conjugated Diolefin hydrocarbon etc., as conventional method, they can only provide unique index, but cannot therefrom obtain the reality of sample
The information of chemical component；In addition, being often difficult the method Accurate Determining with chemical analysis at Oxidation of Fat and Oils initial stage.Such as the prior art
In method that peroxide value is detected have it is following several: (1) titration: according to peroxide in acid (glacial acetic acid) condition
Under can aoxidize potassium iodide, so that iodine be precipitated, because sodium thiosulfate can carry out quantitative reaction with iodine, therefore thiosulfuric acid can be used
The standard solution of sodium titrates the iodine being precipitated, and the peroxidating of grease is calculated according to the volume of consumption sodium thiosulfate standard solution
Value.But by the unknown dominant and plus instruction of the reproducibility of KI work reagent used, the volatility of iodine be precipitated, titration end-point
Agent keeps the operation of this method not only cumbersome but also is not easy to grasp sooner or later to the influence of measurement result.(2) spectrophotometric Colo: according to grease
Middle peroxide oxidation generates to colour solution, finally by measured under 410nm wavelength sample liquid light absorption value and with iodine mark
Quasi- serial solution light absorption value is more quantitative.But this method equally exists that cumbersome, error is larger, the disadvantages of taking a long time,
Be not suitable for fast real-time monitoring.Another chromatography can be thinner such as gas chromatography, this kind of technology of high performance liquid chromatography
Ground research oxidation course is caused, it is also very sensitive to grease mild oxidation, however a large amount of sample process is but needed, equipment operation is multiple
The miscellaneous and a large amount of pretreatment times of consumption, can not achieve the purpose analyzed real-time, quickly.Therefore, it is convenient fast to formulate a kind of Oxidation of Fat and Oils
Speed and for slight oxide in trace quantities since again very sensitive analysis method be very necessary.
Currently, oil oxidative stability method for measuring has: the Oven Method of Schael, Fira Astell kaparabhati,
Sylvester kaparabhati, index method, thermal analysis system (DSC, DTA, TG etc.) method etc..But it is the most applicable and most widely used
Be Swift method, i.e., active oxygen method or be AOM (Active Oxygen Method).This method is will to quantify oil sample to be measured to protect
It holds at 97.8 DEG C, and is passed through the air of certain flow (2.33m1/sec) thereto, bring it about oxidation reaction, while to oil sample
Peroxide value variation carry out dynamic measurement；When oil sample peroxide value reaches 100meq/kg, the time of oxidation is AOM
Value.AOM method is the test method to grow up the thirties, thereafter respectively by American Oil chemist association (AOCS) and state, Britain
Family standard (BS) be set to standard method (AOCS Method Cd12-57 and BS-684Section2.25), and continue to use always to
It is modern.
But AOM method is cumbersome, consumes a large amount of manpower and material resources；When carrying out determination of POV, because using titration
Method, required sample size is larger, therefore when AOM measurement, it need to be reacted simultaneously with multiple reaction tubes, in this way measurement poor repeatability, and
And automatic operation difficult to realize；If measuring the good oil sample of stability, AOM value often reaches tens of hours, in this way measurement personnel
Need to endure tens hours time together with oil sample, labor intensity is very big, so AOM method is not easy to promote the use of.
Summary of the invention
In view of this, the present invention is analyzing a large amount of peroxide value of evening primrose oil and its dielectric constant data relationship
On the basis of, propose a kind of detection method of evening primrose oil oxidation stability, detection method accuracy provided by the invention it is good and
It is easy to operate, it plays an important role to further the improving for Quality Detection standard of evening primrose oil.
The present invention provides a kind of detection methods of evening primrose oil oxidation stability, comprising the following steps:
A) using the quantitative detecting method for the evening primrose oil dielectric constant established respectively to the oenothera biennis of different degree of oxidation
The dielectric constant of oil carries out quantitative detection, obtains the dielectric constant of evening primrose oil and the relationship of degree of oxidation；
B) the dielectric constant progress quantitative detection for surveying oenothera biennis oil samples is treated, testing result is shown in step a) the obtained moon
The dielectric constant of careless oil is compared with the relationship of degree of oxidation, obtains the testing result of evening primrose oil oxidation stability.
Preferably, the quantitative detecting method of the evening primrose oil dielectric constant of foundation described in step a) is physical index analysis
Method.
Preferably, the instrument of physical index analytic approach described in step a) is WGJSTD-A type dielectric loss and dielectric constant
Tester.
Preferably, the temperature of physical index analytic approach described in step a) is 35 DEG C~45 DEG C, and the range of frequency of oscillation is
25kHz~50MHz, stepping be 25kHz~74kHz, 74kHz~213kHz, 213kHz~700kHz, 700kHz~1.95MHz,
1.95MHz~5.2MHz, 5.2MHz~17MHz and seven ranges of 17MHz~50MHz.
Preferably, physical index analytic approach described in step a) includes inductance measurement and capacitance measurement；
The range of the inductance measurement be 0.1 μ H~1H, stepping be 0.1 μ H of μ H~1,1 μ H of μ H~10,10 μ H of μ H~100,
Seven 0.1mH~lmH, 1mH~10mH, 10mH~100mH and 100mH~1H ranges；
The range of the capacitance measurement is 1pF~460pF, and homophony range is 40pF~500pF, and homophony range accuracy is
50pF or less ± 1.5pF, 150pF or more ± 1%, fine setting scope are 0~± 3pF, and fine setting scope accuracy is ± 0.2pF.
Preferably, after the evening primrose oil of difference degree of oxidation described in step a) was including 30 days, 60 DEG C of incubator accelerated oxidations
Non- nitrogen-filled packaging evening primrose oil, 30 days, the evening primrose oil of nitrogen-filled packaging after 60 DEG C of incubator accelerated oxidations and newly refine
Evening primrose oil.
Preferably, described 30 days, the nitrogen charging pressure of the evening primrose oil of nitrogen-filled packaging after 60 DEG C of incubator accelerated oxidations be 0.5
A atmospheric pressure~1.5 atmospheric pressure, nitrogen charging temperature are 10 DEG C~30 DEG C, and nitrogen charging number is 1 time~3 times.
Preferably, the process of quantitative detection described in step a) specifically:
A1 the evening primrose oil of different degree of oxidation) is divided into several groups according to different temperatures, respectively three times to every group of progress
Dielectric constant analysis controls the accuracy of the dielectric constant analysis within 2%, the dielectric constant of evening primrose oil is calculated
Measurement average value；
A2) measured value of the dielectric constant of the obtained evening primrose oil of step a1) is shown in the moon of corresponding degree of oxidation respectively
The peroxide value of careless oil is matched, and the dielectric constant of evening primrose oil and the relationship of degree of oxidation are obtained.
Preferably, step a1) described in different temperatures specifically:
0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C and 60 DEG C.
The present invention also provides a kind of detection methods of evening primrose oil oxidation stability described in above-mentioned technical proposal in the moon
See the application in careless oil product quality detection.
The present invention provides a kind of detection methods of evening primrose oil oxidation stability, comprising the following steps: a) using foundation
The quantitative detecting method of evening primrose oil dielectric constant the dielectric constant of the evening primrose oil of different degree of oxidation is determined respectively
Amount detection, obtains the dielectric constant of evening primrose oil and the relationship of degree of oxidation；B) dielectric constant for surveying oenothera biennis oil samples is treated
Quantitative detection is carried out, testing result is compared with the dielectric constant of the obtained evening primrose oil of step a) and the relationship of degree of oxidation
Compared with obtaining the testing result of evening primrose oil oxidation stability.The present invention is normal to a large amount of peroxide value of evening primrose oil and its dielectric
On the basis of number data relationship is analyzed, a kind of detection method of evening primrose oil oxidation stability is proposed, by measuring the moon
The dielectric constant of careless oil is seen to detect its oxidation stability, while establishing the quantitative detection side of evening primrose oil oxidation stability
Method.Detection method accuracy provided by the invention is good and easy to operate, to the further complete of the Quality Detection standard of evening primrose oil
It is kind to play an important role.
In addition, detection method provided by the invention is applied in the Quality Detection of evening primrose oil, it can find that in time the moon is shown in
The slight oxide in trace quantities since of careless oil, rapidly monitors evening primrose oil Quality Safety stability in time；And it can be realized automation behaviour
Make, reduce human and material resources investment, there is significant contribution to the research field of domestic and international evening primrose oil, push evening primrose oil oxidation
The development of Detection of Stability technology improves vegetable fat quality monitoring ability.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical solution of the present invention is clearly and completely described, it is clear that institute
The embodiment of description is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
The present invention provides a kind of detection methods of evening primrose oil oxidation stability, comprising the following steps:
A) using the quantitative detecting method for the evening primrose oil dielectric constant established respectively to the oenothera biennis of different degree of oxidation
The dielectric constant of oil carries out quantitative detection, obtains the dielectric constant of evening primrose oil and the relationship of degree of oxidation；
B) the dielectric constant progress quantitative detection for surveying oenothera biennis oil samples is treated, testing result is shown in step a) the obtained moon
The dielectric constant of careless oil is compared with the relationship of degree of oxidation, obtains the testing result of evening primrose oil oxidation stability.
The present invention is first using the quantitative detecting method for the evening primrose oil dielectric constant established respectively to different degree of oxidation
Evening primrose oil dielectric constant carry out quantitative detection, obtain the dielectric constant of evening primrose oil and the relationship of degree of oxidation.At this
In invention, the quantitative detecting method of the evening primrose oil dielectric constant of the foundation is preferably physical index analytic approach.In the present invention
In, the instrument of the physical index analytic approach is preferably WGJSTD-A type dielectric loss and dielectrometer, is thought by instrument odd
(Beijing) development in science and technology Co., Ltd provides；The instrumentation is simple, and correction is convenient；Open sonde configuration is easy to clean.
In the present invention, the WGJSTD-A type dielectric loss and dielectrometer cardinal principle are that measurement grease exists
The recommended method of capacitivity and dielectric dissipation factor under power frequency, audio, high frequency (including metric wave wavelength), wherein high-frequency dielectric
Loss test system is by S916 test device (fixture), GDAT type HF Q meter, data acquisition and tan δ automatic measurement control (dress
Enter GDAT) and LKI-1 type inductor composition, which provides the high-frequency dielectric loss tangent (tan δ) of material
With the best solution of dielectric constant (ε) automatic measurement.The present invention is surveyed using WGJSTD-A type dielectric loss and dielectric constant
Instrument is tried under specified conditions and conditional parameter, accurately quantitative detection can go out the dielectric constant of evening primrose oil, accuracy can
Control is within 2%.
In the present invention, the temperature of the physical index analytic approach is preferably 35 DEG C~45 DEG C, more preferably 40 DEG C；It is described
The frequency of oscillation of physical index analytic approach ranges preferably from 25kHz~50MHz, stepping be 25kHz~74kHz, 74kHz~
213kHz, 213kHz~700kHz, 700kHz~1.95MHz, 1.95MHz~5.2MHz, 5.2MHz~17MHz and 17MHz~
Seven ranges of 50MHz.In the preferred embodiment of the invention, the frequency of oscillation selected range of the physical index analytic approach is
700kHz~1.95MHz.
In the present invention, the physical index analytic approach preferably includes inductance measurement and capacitance measurement.In the present invention, institute
The 0.1 μ H~1H that ranges preferably from of inductance measurement is stated, stepping is 0.1 μ H of μ H~1,1 μ H of μ H~10,10 μ H of μ H~100,0.1mH
Seven ranges of~lmH, 1mH~10mH, 10mH~100mH and 100mH~1H.In the preferred embodiment of the invention, the electricity
Sensed quantity selected range is 0.1mH~lmH.
In the present invention, the capacitance measurement ranges preferably from 1pF~460pF, more preferably 100pF~200pF；Institute
The homophony for stating capacitance measurement ranges preferably from 40pF~500pF, more preferably 150pF~220pF；The homophony of the capacitance measurement
Range accuracy is preferably 50pF or less ± 1.5pF, 150pF or more ± 1%；The fine setting scope of the capacitance measurement is preferably 0
~± 3pF；The fine setting scope accuracy of the capacitance measurement is preferably ± 0.2pF.
In the present invention, the evening primrose oil of the different degree of oxidation preferably includes 30 days, after 60 DEG C of incubator accelerated oxidations
Non- nitrogen-filled packaging evening primrose oil, 30 days, the evening primrose oil of nitrogen-filled packaging after 60 DEG C of incubator accelerated oxidations and newly refine
Evening primrose oil；Wherein described 30 days, the nitrogen charging pressure of the evening primrose oil of nitrogen-filled packaging after 60 DEG C of incubator accelerated oxidations be preferably
0.5 atmospheric pressure~1.5 atmospheric pressure, more preferably 1 atmospheric pressure；Described 30 days, the nitrogen charging after 60 DEG C of incubator accelerated oxidations
The nitrogen charging temperature of the evening primrose oil of packaging is preferably 10 DEG C~30 DEG C, more preferably 20 DEG C；Described 30 days, 60 DEG C of incubators acceleration oxygen
The nitrogen charging number of the evening primrose oil of nitrogen-filled packaging after change is preferably 1 time~3 times, more preferably 1 time.The present invention is shown in the moon
The source of careless oil is not particularly limited, using kind evening primrose oil each all over the world well known to those skilled in the art.?
In the present invention, the evening primrose oil is preferably not added with antioxidant.
The present invention is not particularly limited the process of described 30 days, 60 DEG C of incubator accelerated oxidations, using those skilled in the art
The 30 days accelerated oxidations experiment carried out in 60 DEG C of incubators known to member.
In the present invention, the process of the quantitative detection is preferred specifically:
A1 the evening primrose oil of different degree of oxidation) is divided into several groups according to different temperatures, respectively three times to every group of progress
Dielectric constant analysis controls the accuracy of the dielectric constant analysis within 2%, the dielectric constant of evening primrose oil is calculated
Measured value；
A2) measured value of the dielectric constant of the obtained evening primrose oil of step a1) is shown in the moon of corresponding degree of oxidation respectively
The peroxide value of careless oil is matched, and the dielectric constant of evening primrose oil and the relationship of degree of oxidation are obtained.
The evening primrose oil of different degree of oxidation is divided into several groups according to different temperatures by the present invention, respectively to every group of carry out three
Secondary dielectric constant analysis controls the accuracy of the dielectric constant analysis within 2%, and the dielectric that evening primrose oil is calculated is normal
Several measured values.In the present invention, the evening primrose oil of the different degree of oxidation is identical as in above-mentioned technical proposal, herein not
It repeats again.In the present invention, the different temperatures is preferred specifically:
0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C and 60 DEG C；The evening primrose oil of i.e. every kind different degree of oxidation is all distinguished
It is 7 groups according to 0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C and 60 DEG C of temperature setting.
In the present invention, evening primrose oil dielectric constant described in dielectric constant analysis by adopting the above technical scheme
Quantitative detecting method, details are not described herein.
After obtaining the measured value of the dielectric constant of the evening primrose oil, the present invention is by the dielectric constant of obtained evening primrose oil
Measured value matched respectively with the peroxide value of the evening primrose oil of corresponding degree of oxidation, obtain the dielectric constant of evening primrose oil
With the relationship of degree of oxidation.In the present invention, the peroxide value of the evening primrose oil of the corresponding degree of oxidation passes through this field skill
The determination of POV of GB/T 5538-2005 animal and plant fat known to art personnel obtains.
After obtaining the dielectric constant of the evening primrose oil and the relationship of degree of oxidation, the present invention, which treats, surveys oenothera biennis oil samples
Dielectric constant carry out quantitative detection, testing result and the relationship of the obtained dielectric constant of evening primrose oil and degree of oxidation carry out
Compare, obtains the testing result of evening primrose oil oxidation stability.In the present invention, the process of the quantitative detection, condition and ginseng
Number is identical as in above-mentioned technical proposal step a), and details are not described herein.
The present invention mentions on the basis of analyzing a large amount of peroxide value of evening primrose oil and its dielectric constant data relationship
A kind of detection method of evening primrose oil oxidation stability is gone out.In the present invention, for the evening primrose oil aoxidized, oxygen
Changing product is mostly rudimentary fatty acid, aldehydes, ketone isopolarity compound, these polar compounds can reduce Jie of evening primrose oil
Electric constant；Since evening primrose oil is the product by refining, constituent is the mixture of tens kinds of substances, and every kind of substance
Oxidation product concentration it is all very low, therefore the moon is analyzed by the method for a certain specific oxidation product in detection evening primrose oil
See that careless oily degree of oxidation is relatively difficult, because the detection of any substance is all built upon on the basis of certain detection limit.And
Evening primrose oil is because containing many unsaturated fatty acids, therefore freedom inseparable with aoxidizing, therefore generating after evening primrose oil oxidation
Base, rudimentary fatty acid, aldehydes, ketone isopolarity compound product be mostly absolutely it is all related with dielectric constant, by detect the moon see
A certain specific oxidation product cannot achieve in careless oil, but the dielectric constant by testing evening primrose oil can be achieved on
's.And according to above-mentioned analysis it is found that the dielectric constant of evening primrose oil can be by quantitative detection and being calculated, therefore the moon
See that the dielectric constant that careless oil is generated due to oxidation can be using the quantitative detection side of the dielectric constant for the evening primrose oil established
Method is measured.
Detection method provided by the invention detects its oxidation stability by measuring the dielectric constant of evening primrose oil, simultaneously
Establish the quantitative detecting method of evening primrose oil oxidation stability.Detection method accuracy provided by the invention is good and operation is simple
It is single, it plays an important role to further the improving for Quality Detection standard of evening primrose oil.
The present invention also provides a kind of detection methods of evening primrose oil oxidation stability described in above-mentioned technical proposal in the moon
See the application in careless oil product quality detection.In the present invention, the detection method can be applied in evening primrose oil production, transport, storage
In hiding and use process；Detection method provided by the invention is applied in the Quality Detection of evening primrose oil, can find the moon in time
The slight oxide in trace quantities since for seeing careless oil, rapidly monitors evening primrose oil Quality Safety stability in time；And it can be realized automation
Operation reduces human and material resources investment, has significant contribution to the research field of domestic and international evening primrose oil, pushes evening primrose oil inspection
The development of survey technology improves vegetable fat quality monitoring ability.
The present invention provides a kind of detection methods of evening primrose oil oxidation stability, comprising the following steps: a) using foundation
The quantitative detecting method of evening primrose oil dielectric constant the dielectric constant of the evening primrose oil of different degree of oxidation is determined respectively
Amount detection, obtains the dielectric constant of evening primrose oil and the relationship of degree of oxidation；B) dielectric constant for surveying oenothera biennis oil samples is treated
Quantitative detection is carried out, testing result is compared with the dielectric constant of the obtained evening primrose oil of step a) and the relationship of degree of oxidation
Compared with obtaining the testing result of evening primrose oil oxidation stability.The present invention is normal to a large amount of peroxide value of evening primrose oil and its dielectric
On the basis of number data relationship is analyzed, a kind of detection method of evening primrose oil oxidation stability is proposed, by measuring the moon
The dielectric constant of careless oil is seen to detect its oxidation stability, while establishing the quantitative detection side of evening primrose oil oxidation stability
Method.Detection method accuracy provided by the invention is good and easy to operate, to the further complete of the Quality Detection standard of evening primrose oil
It is kind to play an important role.
In addition, detection method provided by the invention is applied in the Quality Detection of evening primrose oil, it can find that in time the moon is shown in
The slight oxide in trace quantities since of careless oil, rapidly monitors evening primrose oil Quality Safety stability in time；And it can be realized automation behaviour
Make, reduce human and material resources investment, there is significant contribution to the research field of domestic and international evening primrose oil, push evening primrose oil detection
The development of technology improves vegetable fat quality monitoring ability.
In order to further illustrate the present invention, it is described in detail below by following embodiment.
Embodiment 1
Measure the dielectric constant of evening primrose oil:
Quantitative detection is carried out to the dielectric constant of the evening primrose oil of different degree of oxidation respectively；The difference degree of oxidation
Evening primrose oil includes:
The evening primrose oil of non-nitrogen-filled packaging after (1) 30 day accelerated oxidation；Nitrogen-filled packaging after (2) 30 days accelerated oxidations
Evening primrose oil；(3) evening primrose oil newly refined；Antioxidant is not used in the above three classes evening primrose oil；Wherein, add within (2) 30 days
The evening primrose oil of nitrogen-filled packaging after speed oxidation carries out nitrogen charging (nitrogen charging pressure is 1 atmospheric pressure) sealing packet afterwards at 20 DEG C
Dress；
The every a kind of temperature all in accordance with 0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C and 60 DEG C of above three classes evening primrose oil
It is set as 7 groups (serial number is followed successively by A, B, C, D, E, F)；Dielectric constant is analyzed three times for every group of progress, controls the dielectric constant point
The accuracy of analysis is within 2%；
The instrument of the dielectric constant analysis is WGJSTD-A type dielectric loss and dielectrometer, and temperature is set as 40
DEG C, frequency of oscillation selects 700kHz~1.95MHz range, and inductance measurement selects 0.1mH~lmH range, the range of capacitance measurement
For 100pF~200pF, homophony range is 150pF~220pF, accuracy be 50pF or less ± 1.5pF, 150pF or more ±
1%, fine setting scope is 0~± 3pF, and accuracy is ± 0.2pF.
The measured value of the dielectric constant of evening primrose oil is calculated again；As a result referring to table 1.
The dielectric constant test result data of the evening primrose oil of the different degree of oxidation of table 1
| Serial number | Evening primrose oil (non-nitrogen charging) | Evening primrose oil (nitrogen charging group) | New refining evening primrose oil |
| A | 4.01 | 2.89 | 1.82 |
| B | 4.03 | 2.93 | 1.86 |
| C | 4.06 | 3.01 | 1.96 |
| D | 4.12 | 3.05 | 1.98 |
| E | 4.28 | 3.18 | 1.99 |
| F | 4.33 | 3.23 | 2.02 |
As shown in Table 1, it is found by the contrast test of the practical evening primrose oil of three classes, the three classes evening primrose oil under the same terms
Dielectric constant relationship are as follows: fresh group of > of non-nitrogen charging group > nitrogen charging group, and significant difference between group, it was confirmed that pass through measurement oenothera biennis
The dielectric constant of oil can be used to characterize the degree of oxidation of evening primrose oil.
Embodiment 2
Practical evening primrose oil analysis experiment:
(1) measured value of the dielectric constant of the evening primrose oil for the different degree of oxidation for obtaining table 1 is aoxidized with corresponding respectively
The peroxide value of the evening primrose oil of degree is matched, and the dielectric constant of evening primrose oil and the relationship of degree of oxidation are obtained；Specifically
Referring to table 2.
Degree of oxidation analysis result data (the peroxide value unit: m mol/ of the evening primrose oil of the different degree of oxidation of table 2
kg)
| Serial number | Evening primrose oil (non-nitrogen charging) | Evening primrose oil (nitrogen charging group) | New refining evening primrose oil |
| A | 2.02 | 1.03 | 0.82 |
| B | 2.05 | 1.13 | 0.86 |
| C | 2.07 | 1.15 | 0.96 |
| D | 2.12 | 1.25 | 0.98 |
| E | 2.28 | 1.34 | 0.99 |
| F | 2.33 | 1.45 | 1.02 |
The peroxide value of the evening primrose oil of the corresponding degree of oxidation passes through GB/T well known to those skilled in the art
The determination of POV of 5538-2005 animal and plant fat obtains.
(2) take the evening primrose oil sample to be measured of different degree of oxidation according to 0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C and
60 DEG C of temperature setting is 7 groups (serial number is followed successively by A, B, C, D, E, F)；Dielectric constant is analyzed three times for every group of progress, described in control
The accuracy of dielectric constant analysis is within 2%；
The instrument of the dielectric constant analysis is WGJSTD-A type dielectric loss and dielectrometer, and temperature is set as 40
DEG C, frequency of oscillation selects 700kHz~1.95MHz range, and inductance measurement selects 0.1mH~lmH range, the range of capacitance measurement
For 100pF~200pF, homophony range is 150pF~220pF, accuracy be 50pF or less ± 1.5pF, 150pF or more ±
1%, fine setting scope is 0~± 3pF, and accuracy is ± 0.2pF.
The measured value of the dielectric constant of evening primrose oil sample to be measured is calculated again；As a result referring to table 3.
The dielectric constant test result data of the practical evening primrose oil of table 3
By in table 3 testing result and step (1) obtained relationship of the dielectric constant of evening primrose oil and degree of oxidation into
Row compares, and obtains the testing result of evening primrose oil sample oxidation stability to be measured；Ginseng is shown in Table 4.
The degree of oxidation analysis result data (peroxide value unit: m mol/kg) of the practical evening primrose oil of table 4
| Serial number | Evening primrose oil |
| A | 0.86 |
| B | 0.96 |
| C | 1.03 |
| D | 1.45 |
| E | 2.07 |
| F | 2.12 |
Meanwhile the present invention is treated using the determination of POV of GB/T 5538-2005 animal and plant fat and surveys oenothera biennis oil sample
The practical peroxide value of product is measured, and is obtained evening primrose oil sample oxidation to be measured with the detection method that embodiment 2 provides and is stablized
Property testing result carry out Accuracy Verification, the results showed that, the embodiment of the present invention 2 provide detection method accuracy it is good；And
The detection method is easy to operate, can be realized automatic operation, human and material resources investment is reduced, to the Quality Detection of evening primrose oil
Further improving for standard plays an important role.
The above description of the disclosed embodiments, enables those skilled in the art to implement or use the present invention.It is right
A variety of modifications of these embodiments will be readily apparent to those skilled in the art, and as defined herein one
As principle can realize in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will
It will not be intended to be limited to the embodiments shown herein, and be to fit to consistent with the principles and novel features disclosed herein
Widest scope.
Claims (10)
1. a kind of detection method of evening primrose oil oxidation stability, comprising the following steps:
A) using the quantitative detecting method for the evening primrose oil dielectric constant established respectively to the evening primrose oil of different degree of oxidation
Dielectric constant carries out quantitative detection, obtains the dielectric constant of evening primrose oil and the relationship of degree of oxidation；
B) the dielectric constant progress quantitative detection for surveying oenothera biennis oil samples, the evening primrose oil that testing result and step a) are obtained are treated
Dielectric constant be compared with the relationship of degree of oxidation, obtain the testing result of evening primrose oil oxidation stability.
2. detection method according to claim 1, which is characterized in that the evening primrose oil dielectric of foundation described in step a) is normal
Several quantitative detecting methods is physical index analytic approach.
3. detection method according to claim 2, which is characterized in that the instrument of physical index analytic approach described in step a)
For WGJSTD-A type dielectric loss and dielectrometer.
4. detection method according to claim 2, which is characterized in that the temperature of physical index analytic approach described in step a)
Be 35 DEG C~45 DEG C, the range of frequency of oscillation is 25kHz~50MHz, stepping is 25kHz~74kHz, 74kHz~213kHz,
213kHz~700kHz, 700kHz~1.95MHz, 1.95MHz~5.2MHz, 5.2MHz~17MHz and 17MHz~50MHz seven
A range.
5. detection method according to claim 2, which is characterized in that physical index analytic approach described in step a) includes electricity
Sensed quantity and capacitance measurement；
The range of the inductance measurement be 0.1 μ H~1H, stepping be 0.1 μ H of μ H~1,1 μ H of μ H~10,10 μ H of μ H~100,
Seven 0.1mH~lmH, 1mH~10mH, 10mH~100mH and 100mH~1H ranges；
The range of the capacitance measurement is 1pF~460pF, and homophony range is 40pF~500pF, and homophony range accuracy is 50pF
± 1.5pF, 150pF or more ± 1% below, fine setting scope are 0~± 3pF, and fine setting scope accuracy is ± 0.2pF.
6. detection method according to claim 1, which is characterized in that the oenothera biennis of difference degree of oxidation described in step a)
Oil includes 30 days, the evening primrose oil of non-nitrogen-filled packaging after 60 DEG C of incubator accelerated oxidations, 30 days, after 60 DEG C of incubator accelerated oxidations
The evening primrose oil of nitrogen-filled packaging and the evening primrose oil newly refined.
7. detection method according to claim 6, which is characterized in that described 30 days, filling after 60 DEG C of incubator accelerated oxidations
The nitrogen charging pressure of the evening primrose oil of nitrogen packaging is 0.5 atmospheric pressure~1.5 atmospheric pressure, and nitrogen charging temperature is 10 DEG C~30 DEG C, is filled
Nitrogen number is 1 time~3 times.
8. detection method according to claim 1, which is characterized in that the process of quantitative detection described in step a) is specific
Are as follows:
A1 the evening primrose oil of different degree of oxidation) is divided into several groups according to different temperatures, respectively to every group of progress dielectric three times
Constant Analysis controls the accuracy of the dielectric constant analysis within 2%, the survey of the dielectric constant of evening primrose oil is calculated
Allocate mean value；
A2) by the measured value of the dielectric constant of the obtained evening primrose oil of step a1) respectively with the evening primrose oil of corresponding degree of oxidation
Peroxide value matched, obtain the dielectric constant of evening primrose oil and the relationship of degree of oxidation.
9. detection method according to claim 8, which is characterized in that step a1) described in different temperatures specifically:
0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C and 60 DEG C.
10. a kind of detection method of evening primrose oil oxidation stability according to any one of claims 1 to 9 is in evening primrose oil quality
Application in detection.
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| EP4191243A3 (en) * | 2021-12-06 | 2023-08-23 | Nippon Pillar Packing Co., Ltd. | Oil state detection apparatus |
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